MD06G1109600.v1.1

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013940
Physical Location & Seq
Forward (+)
24917904 .. 24921432
3529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1109600.v1.1.491

Sequence Viewer

Length: 1947 bp
ATGAAGAAGCCTCGGAACGGCTCCTTTTATACCAAAACCCAAACGAGAAACTACCACACCAGCATTGACGGCTCCAATTTCCAGGAAAGCAACAGTGGTGGAGAAAGCTGCAAAAGCAAAAGTAAGAAATGCTACAACAACAAGAGCAAGAGCTTCTACGAGCTTTCTCTCTCTCTGATCTTCTCTCTCTGGTGCCTTGTATTCTTGTTCTACTCTAAGCTCGGTCTCGGCCATGGCAATGGAGGGAATTCAGCTCCTGATAACAGGTCTACACATTATCCTAGTGTTCACAATGGTAAGCACGGTGACCATGCATGCTCATTTGTAGAAAATGGAAGCGGAAAACAGACAAATGGACTGGTATTGGATTTTACTTCGTCTCAAAGCTGTAACGATTCTGGAGTTTCTGACAACTATGCAATTTCCAAGTACTCACTTCCAGAGACGGACAGATTAGAAAAAATAGTTTGGAGTATTTTAGGCTATGGTGACTTGGTTTGTGAACTACATCAAGCACATGATTATAACACAAATCATTCAGGACAACTTCTGAATGGAGGAACTTCTCATCCCTCCTATCTCAATATTGACGAATTTCGAAACATAACAAAGCAGGAAAAAGGTCAGGATATGCCAAGTCAGCTTATTAACATTACCCAGGGGCTTGAATCTGATGGAATGGAATACAACTATGCCTCTGCATCCAAAGGTGCAAAAGTGGTGGCTCACAATAAGGAAGCAAAAGGAGCATGTAACATATTGGGAAAGGATCACGATAAGTACCTGAGAAACCCTTGTTCCGTCGGGGGAAAGTTTGTTATAGTTGAGCTTGCAGAAGAGACTCTGGTAAATTCTGTCAAGATTGCAAACTTTGAGCACTACTCTTCTAATTTCAAGGAATTTGAATTGTCTGGAAGTTTAAGCTATCCGGCAGAAGCGTGGTTGCCATTGGGCAACTTTGTTGCTGCTAATGTCAAGCATGCTCAGACCTTTAAGCTGCCTGAACCCAAATGGGTAAGATACTTGAAGTTAGATTTACTAAGTCACTATGGGTCTGAGTTTTACTGCACATTAAGTGTTCTAGAGGTGTATGGCATTAACGCAATTGAGCGGATGCTAGAAGATCTGATTGTGGCGCCTGCAGAACCTGCAGCCAATAAATTGGCAGAGCCTAATTCAACTGTACAATTGTCTCCAAGAGAAGAGGTTGGTTCAACTGAAGGGAAAACAAGTAATGAAGGTCAAACTGGGGTTCGAACAGCTGGTGTAGGGACAGAAAGCATTGAAGATACACAAAAGGTCAATGTAGATGTTACCAAAAATCCAGTAACTACGAGCAAGATTCCAGAGCCGGTTCTGAAGGTTAGACAGCAGCCGAATGGAAGAATTCCTGGTGACTCAGTTCTGAAGATTTTAATGCAAAAAGTGAGGTCACTCGAGCTGAACTTAGCTGTGCTGGAGGAATATATCAAAGAATTAAATCGAAGACAGGGGGGTATCTTGCCGGAGGTTGGTAAAGAGCTGTTGAGAATATCATTGCTTCTGGATCAAAGCAAAACGGAGATCAAAGATATCTTGCAATGGAAGGAAATTATGGAAAAAAGTATTACCGACCTTGAATCATGGAAAGCTGCTGTTTCATCCCAAGTGAATGCATTGGCCAGGGAAAATAACATGTTAAGAATAGACATTGAGAAGGTTGTGAAGGATCAAGCGAGCCTCGAAAGCAAAGAGCTTGCGGTTCTAGCAGTTAGTCTTTTCTTTTTGTGCTTTGCAATTCTGAAACTAGTTTCAATGCAAATTTTGACATTTTTAAGAGGAGCCTCCTCCTCCTCCTCCTCGCCGTCTCAGTCTCAGCCTGAGAATGCATGCAGGAACAATAGAGGTTGGGTTTTTATGATTGTTAGCAGCAGTATGACAATATTCATTACTTTGATCTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

649

Amino Acids

71.73

Weight (kDa)

6.59

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 243 - 365 1.1e-32 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 1156
AccB1I GGYRCC 2 cut(s) 192, 1135
AccBSI CCGCTC 1 cut(s) 1111
AccI GTMKAC 1 cut(s) 269
AciI CCGC 3 cut(s) 339, 1111, 1739
AclWI GGATC 3 cut(s) 777, 1554, 1716
AcoI YGGCCR 2 cut(s) 229, 1659
AcsI RAATTY 6 cut(s) 247, 593, 850, 899, 1386, 1800
AcuI CTGAAG 3 cut(s) 1239, 1379, 1427
AcyI GRCGYC 1 cut(s) 1136
AfaI GTAC 3 cut(s) 431, 782, 1185
AfiI CCNNNNNNNGG 2 cut(s) 17, 1511
AflIII ACRYGT 1 cut(s) 1674
AgsI TTSAA 9 cut(s) 668, 895, 905, 1027, 1179, 1215, 1286, 1619, 1794
AhlI ACTAGT 1 cut(s) 1786
AjnI CCWGG 4 cut(s) 81, 657, 1390, 1661
AloI GAACNNNNNNTCC 2 cut(s) 553, 585
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 7 cut(s) 230, 384, 437, 833, 1197, 1851, 1857
AlwI GGATC 3 cut(s) 777, 1554, 1716
AlwNI CAGNNNCTG 2 cut(s) 257, 1148
Ama87I CYCGRG 1 cut(s) 1436
AoxI GGCC 2 cut(s) 229, 1659
ApeKI GCWGC 7 cut(s) 108, 965, 997, 1151, 1372, 1631, 1908
ApoI RAATTY 6 cut(s) 247, 593, 850, 899, 1386, 1800
AspLEI GCGC 1 cut(s) 1138
AsuHPI GGTGA 3 cut(s) 317, 500, 1406
AsuII TTCGAA 2 cut(s) 598, 1255
AvaI CYCGRG 1 cut(s) 1436
BalI TGGCCA 1 cut(s) 1661
BanI GGYRCC 2 cut(s) 192, 1135
BbsI GAAGAC 1 cut(s) 1492
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 7 cut(s) 95, 952, 984, 1163, 1384, 1618, 1920
BccI CCATC 1 cut(s) 668
BceAI ACGGC 3 cut(s) 34, 85, 1828
BciT130I CCWGG 4 cut(s) 83, 659, 1392, 1663
BcoDI GTCTC 7 cut(s) 230, 384, 437, 833, 1197, 1851, 1857
BcuI ACTAGT 1 cut(s) 1786
BfaI CTAG 5 cut(s) 282, 1082, 1118, 1745, 1787
BfmI CTRYAG 3 cut(s) 1140, 1149, 1939
BfoI RGCGCY 1 cut(s) 1139
BfuAI ACCTGC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 1123
BisI GCNGC 7 cut(s) 109, 966, 998, 1152, 1373, 1632, 1909
BlsI GCNGC 7 cut(s) 110, 967, 999, 1153, 1374, 1633, 1910
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 4 cut(s) 83, 659, 1392, 1663
BmeT110I CYCGRG 1 cut(s) 1436
BmiI GGNNCC 5 cut(s) 22, 73, 194, 1137, 1822
BmrFI CCNGG 4 cut(s) 83, 659, 1392, 1663
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 2 cut(s) 710, 1104
BmuI ACTGGG 1 cut(s) 1257
BpiI GAAGAC 1 cut(s) 1492
BplI GAGNNNNNCTC 2 cut(s) 866, 898
BpmI CTGGAG 2 cut(s) 420, 1478
Bpu14I TTCGAA 2 cut(s) 598, 1255
BsaHI GRCGYC 1 cut(s) 1136
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 5 cut(s) 11, 232, 657, 658, 1662
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 1511
Bse118I RCCGGY 1 cut(s) 1351
Bse1I ACTGG 3 cut(s) 363, 1252, 1325
Bse3DI GCAATG 3 cut(s) 244, 1535, 1586
BseBI CCWGG 4 cut(s) 83, 659, 1392, 1663
BseDI CCNNGG 5 cut(s) 11, 232, 657, 658, 1662
BseGI GGATG 4 cut(s) 568, 701, 1119, 1640
BseLI CCNNNNNNNGG 2 cut(s) 17, 1511
BseMI GCAATG 3 cut(s) 244, 1535, 1586
BseMII CTCAG 7 cut(s) 776, 998, 1047, 1413, 1851, 1862, 1868
BseNI ACTGG 3 cut(s) 363, 1252, 1325
BseRI GAGGAG 6 cut(s) 1816, 1819, 1822, 1825, 1828, 1833
BseXI GCAGC 7 cut(s) 95, 952, 984, 1163, 1384, 1618, 1920
BsgI GTGCAG 1 cut(s) 1051
BshFI GGCC 2 cut(s) 231, 1661
BshNI GGYRCC 2 cut(s) 192, 1135
BsiHKAI GWGCWC 1 cut(s) 879
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 3 cut(s) 929, 1352, 1505
BslFI GGGAC 1 cut(s) 1285
BslI CCNNNNNNNGG 2 cut(s) 17, 1511
BsmAI GTCTC 7 cut(s) 230, 384, 437, 833, 1197, 1851, 1857
BsmBI CGTCTC 3 cut(s) 384, 437, 1851
BsmFI GGGAC 1 cut(s) 1285
BsmI GAATGC 2 cut(s) 1657, 1870
BsnI GGCC 2 cut(s) 231, 1661
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 1436
Bsp119I TTCGAA 2 cut(s) 598, 1255
Bsp1286I GDGCHC 1 cut(s) 879
Bsp1407I TGTACA 1 cut(s) 1183
Bsp143I GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 3 cut(s) 339, 1111, 1739
BspANI GGCC 2 cut(s) 231, 1661
BspCNI CTCAG 7 cut(s) 777, 997, 1048, 1412, 1852, 1861, 1867
BspLI GGNNCC 5 cut(s) 22, 73, 194, 1137, 1822
BspMAI CTGCAG 2 cut(s) 1144, 1153
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 3 cut(s) 777, 1554, 1716
BspT104I TTCGAA 2 cut(s) 598, 1255
BspT107I GGYRCC 2 cut(s) 192, 1135
BspTNI GGTCTC 1 cut(s) 230
BsrBI CCGCTC 1 cut(s) 1111
BsrDI GCAATG 3 cut(s) 244, 1535, 1586
BsrFI RCCGGY 1 cut(s) 1351
BsrGI TGTACA 1 cut(s) 1183
BsrI ACTGG 3 cut(s) 363, 1252, 1325
BssAI RCCGGY 1 cut(s) 1351
BssECI CCNNGG 5 cut(s) 11, 232, 657, 658, 1662
BssMI GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
BssNI GRCGYC 1 cut(s) 1136
BssT1I CCWWGG 1 cut(s) 232
Bst2UI CCWGG 4 cut(s) 83, 659, 1392, 1663
Bst4CI ACNGT 3 cut(s) 95, 305, 1183
Bst6I CTCTTC 3 cut(s) 831, 889, 1197
BstACI GRCGYC 1 cut(s) 1136
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstAUI TGTACA 1 cut(s) 1183
BstBI TTCGAA 2 cut(s) 598, 1255
BstC8I GCNNGC 7 cut(s) 316, 831, 981, 1140, 1717, 1737, 1870
BstDSI CCRYGG 1 cut(s) 232
BstEII GGTNACC 1 cut(s) 305
BstF5I GGATG 4 cut(s) 568, 701, 1119, 1640
BstH2I RGCGCY 1 cut(s) 1139
BstHHI GCGC 1 cut(s) 1138
BstKTI GATC 7 cut(s) 180, 772, 1126, 1549, 1566, 1711, 1938
BstMAI GTCTC 7 cut(s) 230, 384, 437, 833, 1197, 1851, 1857
BstMBI GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
BstMWI GCNNNNNNNGC 7 cut(s) 69, 114, 640, 746, 1148, 1725, 1745
BstNI CCWGG 4 cut(s) 83, 659, 1392, 1663
BstNSI RCATGY 5 cut(s) 318, 753, 983, 1678, 1872
BstPI GGTNACC 1 cut(s) 305
BstSCI CCNGG 4 cut(s) 81, 657, 1390, 1661
BstSFI CTRYAG 3 cut(s) 1140, 1149, 1939
BstV1I GCAGC 7 cut(s) 95, 952, 984, 1163, 1384, 1618, 1920
BstV2I GAAGAC 1 cut(s) 1492
BstX2I RGATCY 1 cut(s) 1123
BstXI CCANNNNNNTGG 2 cut(s) 239, 1162
BstYI RGATCY 1 cut(s) 1123
BsuRI GGCC 2 cut(s) 231, 1661
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 4 cut(s) 568, 701, 1119, 1640
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 7 cut(s) 316, 831, 981, 1140, 1717, 1737, 1870
CaiI CAGNNNCTG 2 cut(s) 257, 1148
CfoI GCGC 1 cut(s) 1138
Cfr10I RCCGGY 1 cut(s) 1351
Csp6I GTAC 3 cut(s) 430, 781, 1184
CspCI CAANNNNNGTGG 4 cut(s) 79, 114, 702, 737
CviAII CATG 9 cut(s) 233, 311, 315, 518, 750, 980, 1623, 1675, 1869
CviQI GTAC 3 cut(s) 430, 781, 1184
DinI GGCGCC 1 cut(s) 1137
DpnI GATC 7 cut(s) 179, 771, 1125, 1548, 1565, 1710, 1937
DpnII GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
EaeI YGGCCR 2 cut(s) 229, 1659
Eam1104I CTCTTC 3 cut(s) 831, 889, 1197
EarI CTCTTC 3 cut(s) 831, 889, 1197
Eco130I CCWWGG 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 1573
Eco57I CTGAAG 3 cut(s) 1239, 1379, 1427
Eco88I CYCGRG 1 cut(s) 1436
Eco91I GGTNACC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 305
EcoRI GAATTC 2 cut(s) 247, 1386
EcoRII CCWGG 4 cut(s) 81, 657, 1390, 1661
EcoRV GATATC 1 cut(s) 1573
EcoT14I CCWWGG 1 cut(s) 232
EcoT22I ATGCAT 3 cut(s) 316, 1657, 1870
EgeI GGCGCC 1 cut(s) 1137
EheI GGCGCC 1 cut(s) 1137
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 3 cut(s) 384, 437, 1851
FaeI CATG 9 cut(s) 236, 314, 318, 521, 753, 983, 1626, 1678, 1872
FaqI GGGAC 1 cut(s) 1285
FatI CATG 9 cut(s) 232, 310, 314, 517, 749, 979, 1622, 1674, 1868
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 7 cut(s) 109, 966, 998, 1152, 1373, 1632, 1909
FokI GGATG 4 cut(s) 555, 688, 1126, 1627
Fsp4HI GCNGC 7 cut(s) 109, 966, 998, 1152, 1373, 1632, 1909
FspBI CTAG 5 cut(s) 282, 1082, 1118, 1745, 1787
GlaI GCGC 1 cut(s) 1137
GluI GCNGC 7 cut(s) 109, 966, 998, 1152, 1373, 1632, 1909
GsuI CTGGAG 2 cut(s) 420, 1478
HaeII RGCGCY 1 cut(s) 1139
HaeIII GGCC 2 cut(s) 231, 1661
HapII CCGG 3 cut(s) 929, 1352, 1505
HhaI GCGC 1 cut(s) 1138
Hin1I GRCGYC 1 cut(s) 1136
Hin1II CATG 9 cut(s) 236, 314, 318, 521, 753, 983, 1626, 1678, 1872
Hin6I GCGC 1 cut(s) 1136
HinP1I GCGC 1 cut(s) 1136
HinfI GANTC 6 cut(s) 395, 668, 841, 1342, 1397, 1619
HpaII CCGG 3 cut(s) 929, 1352, 1505
HphI GGTGA 3 cut(s) 317, 500, 1406
Hpy166II GTNNAC 3 cut(s) 270, 289, 503
Hpy8I GTNNAC 3 cut(s) 270, 289, 503
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 6 cut(s) 1214, 1232, 1354, 1579, 1690, 1699
HpyCH4III ACNGT 3 cut(s) 95, 305, 1183
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 114, 640, 746, 1148, 1725, 1745
Hsp92I GRCGYC 1 cut(s) 1136
Hsp92II CATG 9 cut(s) 236, 314, 318, 521, 753, 983, 1626, 1678, 1872
HspAI GCGC 1 cut(s) 1136
KasI GGCGCC 1 cut(s) 1135
Kzo9I GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
LmnI GCTCC 5 cut(s) 26, 77, 259, 746, 1820
Lsp1109I GCAGC 7 cut(s) 95, 952, 984, 1163, 1384, 1618, 1920
LweI GCATC 2 cut(s) 710, 1104
MaeI CTAG 5 cut(s) 282, 1082, 1118, 1745, 1787
MaeIII GTNAC 9 cut(s) 305, 389, 488, 752, 1043, 1312, 1327, 1394, 1431
MalI GATC 7 cut(s) 179, 771, 1125, 1548, 1565, 1710, 1937
MbiI CCGCTC 1 cut(s) 1111
MboI GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
MfeI CAATTG 2 cut(s) 1104, 1187
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 1 cut(s) 879
MlsI TGGCCA 1 cut(s) 1661
MluNI TGGCCA 1 cut(s) 1661
Mly113I GGCGCC 1 cut(s) 1136
MlyI GAGTC 2 cut(s) 835, 1391
Mox20I TGGCCA 1 cut(s) 1661
Mph1103I ATGCAT 3 cut(s) 316, 1657, 1870
MscI TGGCCA 1 cut(s) 1661
MseI TTAA 9 cut(s) 648, 920, 993, 1073, 1098, 1415, 1478, 1679, 1814
MslI CAYNNNNRTG 1 cut(s) 237
Msp20I TGGCCA 1 cut(s) 1661
MspA1I CMGCKG 1 cut(s) 1262
MspI CCGG 3 cut(s) 929, 1352, 1505
MspR9I CCNGG 4 cut(s) 83, 659, 1392, 1663
MunI CAATTG 2 cut(s) 1104, 1187
Mva1269I GAATGC 2 cut(s) 1657, 1870
MvaI CCWGG 4 cut(s) 83, 659, 1392, 1663
MwoI GCNNNNNNNGC 7 cut(s) 69, 114, 640, 746, 1148, 1725, 1745
NarI GGCGCC 1 cut(s) 1136
NcoI CCATGG 1 cut(s) 232
NdeII GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
NlaIII CATG 9 cut(s) 236, 314, 318, 521, 753, 983, 1626, 1678, 1872
NlaIV GGNNCC 5 cut(s) 22, 73, 194, 1137, 1822
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 5 cut(s) 305, 488, 1043, 1394, 1431
NsiI ATGCAT 3 cut(s) 316, 1657, 1870
NspI RCATGY 5 cut(s) 318, 753, 983, 1678, 1872
NspV TTCGAA 2 cut(s) 598, 1255
PaeI GCATGC 3 cut(s) 318, 983, 1872
PaeR7I CTCGAG 1 cut(s) 1436
PasI CCCWGGG 1 cut(s) 658
PciI ACATGT 1 cut(s) 1674
PctI GAATGC 2 cut(s) 1657, 1870
PfeI GAWTC 4 cut(s) 395, 668, 1342, 1619
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 7 cut(s) 110, 967, 999, 1153, 1374, 1633, 1910
PleI GAGTC 2 cut(s) 835, 1391
PluTI GGCGCC 1 cut(s) 1139
PpsI GAGTC 2 cut(s) 835, 1391
PscI ACATGT 1 cut(s) 1674
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 4 cut(s) 81, 657, 1390, 1661
PspEI GGTNACC 1 cut(s) 305
PspGI CCWGG 4 cut(s) 81, 657, 1390, 1661
PspN4I GGNNCC 5 cut(s) 22, 73, 194, 1137, 1822
PspXI VCTCGAGB 1 cut(s) 1436
PstI CTGCAG 2 cut(s) 1144, 1153
PstNI CAGNNNCTG 2 cut(s) 257, 1148
PsuI RGATCY 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 3 cut(s) 431, 782, 1185
RsaNI GTAC 3 cut(s) 430, 781, 1184
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 9 cut(s) 648, 920, 993, 1073, 1098, 1415, 1478, 1679, 1814
SatI GCNGC 7 cut(s) 109, 966, 998, 1152, 1373, 1632, 1909
Sau3AI GATC 7 cut(s) 177, 769, 1123, 1546, 1563, 1708, 1935
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 2 cut(s) 835, 1391
ScrFI CCNGG 4 cut(s) 83, 659, 1392, 1663
SduI GDGCHC 1 cut(s) 879
SfaNI GCATC 2 cut(s) 710, 1104
SfcI CTRYAG 3 cut(s) 1140, 1149, 1939
SfoI GGCGCC 1 cut(s) 1137
Sfr274I CTCGAG 1 cut(s) 1436
SfuI TTCGAA 2 cut(s) 598, 1255
SlaI CTCGAG 1 cut(s) 1436
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 1436
SmoI CTYRAG 1 cut(s) 1436
SpeI ACTAGT 1 cut(s) 1786
SphI GCATGC 3 cut(s) 318, 983, 1872
SsiI CCGC 3 cut(s) 339, 1111, 1739
SspDI GGCGCC 1 cut(s) 1135
SspI AATATT 2 cut(s) 586, 1923
SspMI CTAG 5 cut(s) 282, 1082, 1118, 1745, 1787
StyD4I CCNGG 4 cut(s) 81, 657, 1390, 1661
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 3 cut(s) 95, 305, 1183
TaqI TCGA 5 cut(s) 598, 1255, 1437, 1483, 1722
TaqII GACCGA 1 cut(s) 212
TatI WGTACW 2 cut(s) 429, 1183
TfiI GAWTC 4 cut(s) 395, 668, 1342, 1619
Tru1I TTAA 9 cut(s) 648, 920, 993, 1073, 1098, 1415, 1478, 1679, 1814
Tru9I TTAA 9 cut(s) 648, 920, 993, 1073, 1098, 1415, 1478, 1679, 1814
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 5 cut(s) 305, 488, 1043, 1394, 1431
TseI GCWGC 7 cut(s) 108, 965, 997, 1151, 1372, 1631, 1908
Tsp45I GTSAC 5 cut(s) 305, 488, 1043, 1394, 1431
TspDTI ATGAA 4 cut(s) 17, 1251, 1629, 1915
TspGWI ACGGA 3 cut(s) 461, 790, 1574
TspRI CASTG 1 cut(s) 100
XapI RAATTY 6 cut(s) 247, 593, 850, 899, 1386, 1800
XbaI TCTAGA 1 cut(s) 1081
XceI RCATGY 5 cut(s) 318, 753, 983, 1678, 1872
XhoI CTCGAG 1 cut(s) 1436
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 5 cut(s) 282, 1082, 1118, 1745, 1787
ZrmI AGTACT 1 cut(s) 431
Zsp2I ATGCAT 3 cut(s) 316, 1657, 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.