MD14G1130600.v1.1

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013940
Physical Location & Seq
Forward (+)
20832471 .. 20834570
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1130600.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGCAATGCCATGTGGCAATGCGTGATAAGTTTGGACACAGCCTTGGGCGTCCATGTTTCATCAAGAGCTTCTACGAGCTCTCTCTCTCTTTGATCTTCTCTCTCTGGTGCCTTGTCTTCCCATTCTACTCTAAGCTTGGTCTCGGCCATGGCAATGGAGGGACTTCAGCTCCTGATAACAGATCTACACATTGTCCTAGTGTTCACAGTGGTAAGCAAGGTGACGATGCATGCTCATTTATAGAAAATGGAAGCAGAAAGCAGACAAATGGAATGGTATTAGATTTTACTTCATCTCAAAACTGTTACGATTCTGGAGTTTGTGACAACTATGCAATTTCCAAGTACTCACTTCCAGAGACGAATAGATTAGTTTGGAGTATTTTAGGCTATGGTGATTTGGTTTGTGAACTACATCAAGCACCAGATCACAACACAAATCATTCAGGACAACTGAATGGAAGAACTTCTCATCCCTCCTATCTCAATTTTGACGAATTTCGAAACATAACAAAGCAGGAAAAAGGTCAGGATATCCCTAGTCTGCTAGTTAACGTAACCCACGGGCTTGAATCTGATGGAACAGAGTGCAACTATGCCTCTGCATCCAAGGGTGCAAAAGCGGTGGCTCACAATAAAGAAGCAAAAGGAGCAAGCAACATATTGGGAAAGGATCACGATAAGTGCCTTAGAAACCTTTGCTCTGTTGGGGGAAAGTTCGTCATAGTTGAGCTTGCAGAAGAGACTTTGGTAGATGCTGTCAAGATTGCAAACTTTGAGCACTACTCTTCTAACTTCAAGGAATTTGAATTGTCTGGAAGCTTAAGCTATCCGGCAGAAGCGTGGTCGCCATTGGGAAACTTTGAGGACTACTCTGCAGAACATGCAGCCAATAAATTGCCAGACCCTAATTCAACTGTAAAATCCTCTCCAAAAGAAGAGGTTGGTTCAACTGACTGGAAAACAAGTAGTGTTGGTCAAACTGGGGTTCAAACAGCTGGTGTAGGGACAGAAAGCGTTGAAGATACACAAAAAGTCAATGTAGACATTACCAACAATCCAGTAACTGCAAGCAAGATTCCAGAGCCGGTTATGAAGGTAAGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

370

Amino Acids

40.16

Weight (kDa)

5.77

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 211 - 292 2.2e-14 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 1044
AciI CCGC 1 cut(s) 623
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 2 cut(s) 497, 803
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 1 cut(s) 347
AflII CTTAAG 1 cut(s) 823
AgsI TTSAA 7 cut(s) 572, 799, 809, 915, 951, 992, 1022
AloI GAACNNNNNNTCC 2 cut(s) 457, 489
AluBI AGCT 8 cut(s) 69, 79, 136, 170, 733, 822, 828, 998
AluI AGCT 8 cut(s) 69, 79, 136, 170, 733, 822, 828, 998
Alw21I GWGCWC 2 cut(s) 81, 783
Alw26I GTCTC 3 cut(s) 146, 353, 737
AlwI GGATC 1 cut(s) 681
AlwNI CAGNNNCTG 2 cut(s) 173, 1067
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 887
ApoI RAATTY 2 cut(s) 497, 803
Asp700I GAANNNNTTC 1 cut(s) 466
AsuHPI GGTGA 2 cut(s) 233, 407
AsuII TTCGAA 1 cut(s) 502
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 2 cut(s) 81, 783
BbvI GCAGC 1 cut(s) 899
BccI CCATC 1 cut(s) 572
BcoDI GTCTC 3 cut(s) 146, 353, 737
BfaI CTAG 3 cut(s) 198, 540, 548
BfmI CTRYAG 1 cut(s) 876
BfrI CTTAAG 1 cut(s) 823
BglII AGATCT 1 cut(s) 182
BisI GCNGC 1 cut(s) 888
BlsI GCNGC 1 cut(s) 889
BmcAI AGTACT 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 3 cut(s) 217, 614, 745
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 1 cut(s) 109
BplI GAGNNNNNCTC 4 cut(s) 770, 802, 857, 889
BpmI CTGGAG 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 502
BsaHI GRCGYC 1 cut(s) 49
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 4 cut(s) 43, 148, 562, 609
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bse118I RCCGGY 1 cut(s) 1087
Bse1I ACTGG 3 cut(s) 962, 988, 1061
Bse3DI GCAATG 3 cut(s) 11, 24, 160
BseDI CCNNGG 4 cut(s) 43, 148, 562, 609
BseGI GGATG 2 cut(s) 472, 605
BseMI GCAATG 3 cut(s) 11, 24, 160
BseNI ACTGG 3 cut(s) 962, 988, 1061
BseXI GCAGC 1 cut(s) 899
BshFI GGCC 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 81, 783
BsiSI CCGG 2 cut(s) 833, 1088
BslFI GGGAC 2 cut(s) 175, 1021
BsmAI GTCTC 3 cut(s) 146, 353, 737
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 175, 1021
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 502
Bsp1286I GDGCHC 2 cut(s) 81, 783
Bsp143I GATC 4 cut(s) 93, 182, 427, 673
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 1 cut(s) 623
BspANI GGCC 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 1 cut(s) 681
BspT104I TTCGAA 1 cut(s) 502
BspT107I GGYRCC 1 cut(s) 108
BspTI CTTAAG 1 cut(s) 823
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 3 cut(s) 11, 24, 160
BsrFI RCCGGY 1 cut(s) 1087
BsrI ACTGG 3 cut(s) 962, 988, 1061
BssAI RCCGGY 1 cut(s) 1087
BssECI CCNNGG 4 cut(s) 43, 148, 562, 609
BssMI GATC 4 cut(s) 93, 182, 427, 673
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 3 cut(s) 43, 148, 609
Bst4CI ACNGT 4 cut(s) 209, 305, 919, 1107
Bst6I CTCTTC 3 cut(s) 735, 793, 933
BstACI GRCGYC 1 cut(s) 49
BstAFI CTTAAG 1 cut(s) 823
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 502
BstC8I GCNNGC 4 cut(s) 232, 655, 735, 1072
BstDEI CTNAG 2 cut(s) 132, 689
BstDSI CCRYGG 2 cut(s) 148, 562
BstF5I GGATG 2 cut(s) 472, 605
BstKTI GATC 4 cut(s) 96, 185, 430, 676
BstMAI GTCTC 3 cut(s) 146, 353, 737
BstMBI GATC 4 cut(s) 93, 182, 427, 673
BstMWI GCNNNNNNNGC 2 cut(s) 650, 884
BstNSI RCATGY 2 cut(s) 234, 887
BstSFI CTRYAG 1 cut(s) 876
BstV1I GCAGC 1 cut(s) 899
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 182
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 1 cut(s) 147
BtgI CCRYGG 2 cut(s) 148, 562
BtsCI GGATG 2 cut(s) 472, 605
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 232, 655, 735, 1072
CaiI CAGNNNCTG 2 cut(s) 173, 1067
Cfr10I RCCGGY 1 cut(s) 1087
CseI GACGC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 346
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 5 cut(s) 11, 54, 149, 231, 884
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 2 cut(s) 132, 689
DpnI GATC 4 cut(s) 95, 184, 429, 675
DpnII GATC 4 cut(s) 93, 182, 427, 673
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 3 cut(s) 735, 793, 933
EarI CTCTTC 3 cut(s) 735, 793, 933
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 43, 148, 609
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 79
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 3 cut(s) 43, 148, 609
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 3 cut(s) 43, 148, 609
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 5 cut(s) 14, 57, 152, 234, 887
FaqI GGGAC 2 cut(s) 175, 1021
FatI CATG 5 cut(s) 10, 53, 148, 230, 883
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 1 cut(s) 888
FokI GGATG 2 cut(s) 459, 592
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 888
FspBI CTAG 3 cut(s) 198, 540, 548
GluI GCNGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 1 cut(s) 147
HapII CCGG 2 cut(s) 833, 1088
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 5 cut(s) 14, 57, 152, 234, 887
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 2 cut(s) 134, 820
HinfI GANTC 3 cut(s) 311, 572, 1078
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 2 cut(s) 833, 1088
HphI GGTGA 2 cut(s) 233, 407
Hpy166II GTNNAC 4 cut(s) 205, 410, 553, 1045
Hpy188I TCNGA 1 cut(s) 577
Hpy8I GTNNAC 4 cut(s) 205, 410, 553, 1045
HpyAV CCTTC 1 cut(s) 1090
HpyCH4III ACNGT 4 cut(s) 209, 305, 919, 1107
HpyCH4IV ACGT 1 cut(s) 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 650, 884
HpyF3I CTNAG 2 cut(s) 132, 689
HpySE526I ACGT 1 cut(s) 555
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 5 cut(s) 14, 57, 152, 234, 887
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 4 cut(s) 93, 182, 427, 673
LmnI GCTCC 2 cut(s) 175, 650
Lsp1109I GCAGC 1 cut(s) 899
LweI GCATC 3 cut(s) 217, 614, 745
MaeI CTAG 3 cut(s) 198, 540, 548
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 5 cut(s) 221, 305, 323, 556, 1063
MalI GATC 4 cut(s) 95, 184, 429, 675
MboI GATC 4 cut(s) 93, 182, 427, 673
MboII GAAGA 7 cut(s) 88, 109, 474, 752, 780, 950, 1034
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 2 cut(s) 81, 783
MluCI AATT 7 cut(s) 336, 487, 497, 803, 809, 896, 910
MnlI CCTC 6 cut(s) 152, 487, 610, 859, 934, 937
Mph1103I ATGCAT 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 2 cut(s) 552, 824
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 2 cut(s) 833, 1088
MwoI GCNNNNNNNGC 2 cut(s) 650, 884
NcoI CCATGG 1 cut(s) 148
NdeII GATC 4 cut(s) 93, 182, 427, 673
NlaIII CATG 5 cut(s) 14, 57, 152, 234, 887
NlaIV GGNNCC 1 cut(s) 110
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 221, 323
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 2 cut(s) 234, 887
NspV TTCGAA 1 cut(s) 502
PaeI GCATGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 3 cut(s) 311, 572, 1078
PkrI GCNGC 1 cut(s) 889
Psp124BI GAGCTC 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 110
PstI CTGCAG 1 cut(s) 880
PstNI CAGNNNCTG 2 cut(s) 173, 1067
PsuI RGATCY 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 1 cut(s) 153
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 552, 824
SatI GCNGC 1 cut(s) 888
Sau3AI GATC 4 cut(s) 93, 182, 427, 673
ScaI AGTACT 1 cut(s) 347
SduI GDGCHC 2 cut(s) 81, 783
SfaNI GCATC 3 cut(s) 217, 614, 745
SfcI CTRYAG 1 cut(s) 876
SfuI TTCGAA 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
SphI GCATGC 1 cut(s) 234
Sse9I AATT 7 cut(s) 336, 487, 497, 803, 809, 896, 910
SsiI CCGC 1 cut(s) 623
SspMI CTAG 3 cut(s) 198, 540, 548
SstI GAGCTC 1 cut(s) 81
StyI CCWWGG 3 cut(s) 43, 148, 609
TaaI ACNGT 4 cut(s) 209, 305, 919, 1107
TaiI ACGT 1 cut(s) 558
TaqI TCGA 1 cut(s) 502
TasI AATT 7 cut(s) 336, 487, 497, 803, 809, 896, 910
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 3 cut(s) 311, 572, 1078
Tru1I TTAA 2 cut(s) 552, 824
Tru9I TTAA 2 cut(s) 552, 824
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 2 cut(s) 221, 323
TseI GCWGC 1 cut(s) 887
Tsp45I GTSAC 2 cut(s) 221, 323
TspDTI ATGAA 3 cut(s) 49, 282, 1109
TspRI CASTG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 823
XapI RAATTY 2 cut(s) 497, 803
XceI RCATGY 2 cut(s) 234, 887
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 3 cut(s) 198, 540, 548
ZrmI AGTACT 1 cut(s) 347
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.