pycom06g10320

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013940
Physical Location & Seq
Forward (+)
15657770 .. 15660868
3099 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10320.3

Sequence Viewer

Length: 1869 bp
ATGAAGAAGCCTCGGAACGGCTCCTTTTATACCAAAACCCAAACGAGAGACCACCACACCAGTATTGACGGCTCCAATTTCCAGGAAAGCTGCAGTTTTCAGAAAAGCACTGGGGGAGAAAGCTGCAAAAGCAAAAGTAAGAAATGCTACAACAACAAGAGCAAGAGCTTCTACGAGCTTTCTCTCTCTCTGATCTTCTCTCTCTGGTGCCTTGTATTCTTGTTCTACTCTAAGCTCGGTCTCGGCCATGGCAATGGAGGGAATTCAGCTCCTGATAACAGATCTACACATTATCCTAGTGTTCACAATGGTAAGCACGGTGACCATGCATGCTCATTTGTAGAAAATGGAAGCGGAAAACAGACAAATGGACTGGTATTGGATTTTACTTCGTCTCAAAGCTGTAACGATTCTGGAGTTTCTGACAACTATGCAATTTCCAAGTACTCACTTCCAGAGACGGATAGATTAGAAAAAATAGTTTGGAGTATTTTAGGCTATGGTGATTTGGTTTGTGAACTACATCAAGCACATGATTATAACACAAATCATTCAGGACAACTTCTGAATGGAGGAACTTCTCATCCCTCCTATCTCAATTTTGACGAATTTCAAAACATAACAAAGCAGGAAAAAGGTCAGGATATGCCTAGTCAGCTTGTTAACATTACCCAGGGGCTTGAATCTGATGGAACGGAATACAACTATGCCTCTGCATCCAAAGGTGCAAAAGTGGTGGCTCACAATAAGGAAGCAAAAGGAGCATGTAACATATTGGGAAAGGATCACGATAAGTACCTGAGAAACCCTTGTTCCGTCGGGGGAAAGTTTGTTATAGTTGAGCTTGCAGAAGAGACTCTGGTAAATTCTGTCAAGATTGCAAACTTCGAGCACTACTCTTCTAATTTCAAGGAATTTGAATTGTCTGGAAGTTTAAGCTATCCGGCAGAAGCGTGGACGCCATTGGGAAACTTTGTTGCTGCTAATGTCAAGCATGCTCAGACCTTTAAGCTGCCTGAACCCAAATGGGTAAGATACTTGAAGTTAGATTTACTAAGTCACTACGGGTCAGAGTTTTACTGCACATTAAGTGTTCTAGAGGTGTATGGCATTAACGCAATTGAGCGGATGCTTGAAGATCTGATTGTGGCGCCTGCAGAACCTGCAGCCAATAAATTGGCAGAGCCTAATTCAACTGTACAATCATCTCCAAGAGAAGAGGTTGGTTCAACTGATGGGAAAACAAGTAATGAAGGTCAAACTGGGGTTCGAACAGCTGGTGTAGGAATAGAAAGCGTTGAAGATACACAAAAAGTCAATGTAGATGTTACCAAAAATCCAATAACTACGAGCAAGATTCCAGAGCCGGTTCTGAAGGTTAGACAGCAGCCGAACGGAAGAATTCCTGGTGACTCAGTTCTGAAGATTTTGATGCAAAAAGTGAGGTCACTCGAGCTGAACTTAGCTGTGCTGGAGGAGTATATCAAAGAATTAAATCGAAGACAAGGGGGTATCTTGCCGGAGGAAAAAAGTATTACCGACCTTGAATCATGGAAAGCTGTTGTTTCATCCCAAGTGAATGCATTGGCCAGGGAAAATAACATAATAGACATTGAGAAGGTTGTGAAGGATCAAGCGAGCCTCGAAAGCAAAGAGCTTGCAGTTCTAGCGATTAGTCTTTTCTTTTTGTGCTTTGCAATTCTGAAACTAGTTTCAATGCAAATTTTGACATTTTTAAGAGGAGCCTCCTCCTCCTCGTCCTGGCCGTCTCAGTCTCAGCCTGAGAATGCATGCAGGAACAATAGAGGTTGGGTTTTGATGATTGTTAGCAGCAGCATGACAATATTCATTACTTTGATCTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

623

Amino Acids

68.6

Weight (kDa)

6.37

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 1171
AccB1I GGYRCC 2 cut(s) 207, 1150
AccBSI CCGCTC 1 cut(s) 1126
AciI CCGC 2 cut(s) 354, 1126
AclWI GGATC 2 cut(s) 792, 1638
AcoI YGGCCR 3 cut(s) 244, 1587, 1763
AcsI RAATTY 6 cut(s) 262, 608, 865, 914, 1401, 1722
AcuI CTGAAG 2 cut(s) 1394, 1442
AcyI GRCGYC 2 cut(s) 959, 1151
AfaI GTAC 3 cut(s) 446, 797, 1200
AfiI CCNNNNNNNGG 2 cut(s) 17, 1761
AhlI ACTAGT 1 cut(s) 1708
AjnI CCWGG 5 cut(s) 81, 672, 1405, 1589, 1760
AloI GAACNNNNNNTCC 2 cut(s) 568, 600
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 7 cut(s) 42, 245, 399, 452, 848, 1773, 1779
AlwI GGATC 2 cut(s) 792, 1638
AlwNI CAGNNNCTG 2 cut(s) 272, 1163
Ama87I CYCGRG 1 cut(s) 1451
AoxI GGCC 3 cut(s) 244, 1587, 1763
ApeKI GCWGC 8 cut(s) 90, 123, 980, 1012, 1166, 1387, 1830, 1833
ApoI RAATTY 6 cut(s) 262, 608, 865, 914, 1401, 1722
AspLEI GCGC 1 cut(s) 1153
AsuHPI GGTGA 3 cut(s) 332, 515, 1421
AsuII TTCGAA 1 cut(s) 1270
AvaI CYCGRG 1 cut(s) 1451
BalI TGGCCA 1 cut(s) 1589
BanI GGYRCC 2 cut(s) 207, 1150
BbsI GAAGAC 1 cut(s) 1507
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 8 cut(s) 77, 110, 967, 999, 1178, 1399, 1842, 1845
BccI CCATC 2 cut(s) 683, 1229
BceAI ACGGC 3 cut(s) 34, 85, 1750
BciT130I CCWGG 5 cut(s) 83, 674, 1407, 1591, 1762
BcoDI GTCTC 7 cut(s) 42, 245, 399, 452, 848, 1773, 1779
BcuI ACTAGT 1 cut(s) 1708
BfaI CTAG 5 cut(s) 297, 651, 1097, 1667, 1709
BfmI CTRYAG 4 cut(s) 91, 1155, 1164, 1861
BfoI RGCGCY 1 cut(s) 1154
BfuAI ACCTGC 1 cut(s) 1171
BglII AGATCT 2 cut(s) 281, 1138
BisI GCNGC 8 cut(s) 91, 124, 981, 1013, 1167, 1388, 1831, 1834
BlsI GCNGC 8 cut(s) 92, 125, 982, 1014, 1168, 1389, 1832, 1835
BmcAI AGTACT 1 cut(s) 446
Bme1390I CCNGG 5 cut(s) 83, 674, 1407, 1591, 1762
BmeT110I CYCGRG 1 cut(s) 1451
BmiI GGNNCC 5 cut(s) 22, 73, 209, 1152, 1744
BmrFI CCNGG 5 cut(s) 83, 674, 1407, 1591, 1762
BmrI ACTGGG 2 cut(s) 120, 1272
BmsI GCATC 3 cut(s) 725, 1119, 1422
BmuI ACTGGG 2 cut(s) 120, 1272
BpiI GAAGAC 1 cut(s) 1507
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 2 cut(s) 435, 1493
Bpu14I TTCGAA 1 cut(s) 1270
BsaHI GRCGYC 2 cut(s) 959, 1151
BsaI GGTCTC 2 cut(s) 42, 245
BsaJI CCNNGG 5 cut(s) 11, 247, 672, 673, 1590
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 1761
Bse118I RCCGGY 1 cut(s) 1366
Bse1I ACTGG 4 cut(s) 60, 115, 378, 1267
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 5 cut(s) 83, 674, 1407, 1591, 1762
BseDI CCNNGG 5 cut(s) 11, 247, 672, 673, 1590
BseGI GGATG 4 cut(s) 583, 716, 1134, 1568
BseLI CCNNNNNNNGG 2 cut(s) 17, 1761
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 6 cut(s) 791, 1013, 1428, 1773, 1784, 1790
BseNI ACTGG 4 cut(s) 60, 115, 378, 1267
BseRI GAGGAG 5 cut(s) 1490, 1738, 1741, 1744, 1755
BseXI GCAGC 8 cut(s) 77, 110, 967, 999, 1178, 1399, 1842, 1845
BsgI GTGCAG 1 cut(s) 1066
BshFI GGCC 3 cut(s) 246, 1589, 1765
BshNI GGYRCC 2 cut(s) 207, 1150
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 1451
BsiSI CCGG 3 cut(s) 944, 1367, 1520
BslI CCNNNNNNNGG 2 cut(s) 17, 1761
BsmAI GTCTC 7 cut(s) 42, 245, 399, 452, 848, 1773, 1779
BsmBI CGTCTC 3 cut(s) 399, 452, 1773
BsmI GAATGC 2 cut(s) 1585, 1792
BsnI GGCC 3 cut(s) 246, 1589, 1765
Bso31I GGTCTC 2 cut(s) 42, 245
BsoBI CYCGRG 1 cut(s) 1451
Bsp119I TTCGAA 1 cut(s) 1270
Bsp1286I GDGCHC 1 cut(s) 894
Bsp1407I TGTACA 1 cut(s) 1198
Bsp143I GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 2 cut(s) 354, 1126
BspANI GGCC 3 cut(s) 246, 1589, 1765
BspCNI CTCAG 6 cut(s) 792, 1012, 1427, 1774, 1783, 1789
BspLI GGNNCC 5 cut(s) 22, 73, 209, 1152, 1744
BspMAI CTGCAG 3 cut(s) 95, 1159, 1168
BspMI ACCTGC 1 cut(s) 1171
BspPI GGATC 2 cut(s) 792, 1638
BspT104I TTCGAA 1 cut(s) 1270
BspT107I GGYRCC 2 cut(s) 207, 1150
BspTNI GGTCTC 2 cut(s) 42, 245
BsrBI CCGCTC 1 cut(s) 1126
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 1366
BsrGI TGTACA 1 cut(s) 1198
BsrI ACTGG 4 cut(s) 60, 115, 378, 1267
BssAI RCCGGY 1 cut(s) 1366
BssECI CCNNGG 5 cut(s) 11, 247, 672, 673, 1590
BssMI GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
BssNI GRCGYC 2 cut(s) 959, 1151
BssT1I CCWWGG 1 cut(s) 247
Bst2UI CCWGG 5 cut(s) 83, 674, 1407, 1591, 1762
Bst4CI ACNGT 2 cut(s) 320, 1198
Bst6I CTCTTC 3 cut(s) 846, 904, 1212
BstACI GRCGYC 2 cut(s) 959, 1151
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstAUI TGTACA 1 cut(s) 1198
BstBI TTCGAA 1 cut(s) 1270
BstC8I GCNNGC 7 cut(s) 331, 846, 996, 1155, 1639, 1659, 1792
BstDEI CTNAG 9 cut(s) 231, 800, 999, 1055, 1414, 1462, 1770, 1776, 1782
BstDSI CCRYGG 1 cut(s) 247
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 4 cut(s) 583, 716, 1134, 1568
BstH2I RGCGCY 1 cut(s) 1154
BstHHI GCGC 1 cut(s) 1153
BstKTI GATC 6 cut(s) 195, 284, 787, 1141, 1633, 1860
BstMAI GTCTC 7 cut(s) 42, 245, 399, 452, 848, 1773, 1779
BstMBI GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
BstMWI GCNNNNNNNGC 6 cut(s) 129, 655, 761, 1163, 1647, 1667
BstNI CCWGG 5 cut(s) 83, 674, 1407, 1591, 1762
BstNSI RCATGY 4 cut(s) 333, 768, 998, 1794
BstPI GGTNACC 1 cut(s) 320
BstSCI CCNGG 5 cut(s) 81, 672, 1405, 1589, 1760
BstSFI CTRYAG 4 cut(s) 91, 1155, 1164, 1861
BstV1I GCAGC 8 cut(s) 77, 110, 967, 999, 1178, 1399, 1842, 1845
BstV2I GAAGAC 1 cut(s) 1507
BstX2I RGATCY 2 cut(s) 281, 1138
BstXI CCANNNNNNTGG 2 cut(s) 254, 1177
BstYI RGATCY 2 cut(s) 281, 1138
BsuRI GGCC 3 cut(s) 246, 1589, 1765
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 4 cut(s) 583, 716, 1134, 1568
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 7 cut(s) 331, 846, 996, 1155, 1639, 1659, 1792
CaiI CAGNNNCTG 2 cut(s) 272, 1163
CfoI GCGC 1 cut(s) 1153
Cfr10I RCCGGY 1 cut(s) 1366
CseI GACGC 1 cut(s) 967
Csp6I GTAC 3 cut(s) 445, 796, 1199
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 9 cut(s) 248, 326, 330, 533, 765, 995, 1551, 1791, 1837
CviQI GTAC 3 cut(s) 445, 796, 1199
DdeI CTNAG 9 cut(s) 231, 800, 999, 1055, 1414, 1462, 1770, 1776, 1782
DinI GGCGCC 1 cut(s) 1152
DpnI GATC 6 cut(s) 194, 283, 786, 1140, 1632, 1859
DpnII GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
EaeI YGGCCR 3 cut(s) 244, 1587, 1763
Eam1104I CTCTTC 3 cut(s) 846, 904, 1212
EarI CTCTTC 3 cut(s) 846, 904, 1212
Eco130I CCWWGG 1 cut(s) 247
Eco31I GGTCTC 2 cut(s) 42, 245
Eco57I CTGAAG 2 cut(s) 1394, 1442
Eco88I CYCGRG 1 cut(s) 1451
Eco91I GGTNACC 1 cut(s) 320
EcoO65I GGTNACC 1 cut(s) 320
EcoRI GAATTC 2 cut(s) 262, 1401
EcoRII CCWGG 5 cut(s) 81, 672, 1405, 1589, 1760
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 3 cut(s) 331, 1585, 1792
EgeI GGCGCC 1 cut(s) 1152
EheI GGCGCC 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 247
Esp3I CGTCTC 3 cut(s) 399, 452, 1773
FaeI CATG 9 cut(s) 251, 329, 333, 536, 768, 998, 1554, 1794, 1840
FatI CATG 9 cut(s) 247, 325, 329, 532, 764, 994, 1550, 1790, 1836
Fnu4HI GCNGC 8 cut(s) 91, 124, 981, 1013, 1167, 1388, 1831, 1834
FokI GGATG 4 cut(s) 570, 703, 1141, 1555
Fsp4HI GCNGC 8 cut(s) 91, 124, 981, 1013, 1167, 1388, 1831, 1834
FspBI CTAG 5 cut(s) 297, 651, 1097, 1667, 1709
GlaI GCGC 1 cut(s) 1152
GluI GCNGC 8 cut(s) 91, 124, 981, 1013, 1167, 1388, 1831, 1834
GsuI CTGGAG 2 cut(s) 435, 1493
HaeII RGCGCY 1 cut(s) 1154
HaeIII GGCC 3 cut(s) 246, 1589, 1765
HapII CCGG 3 cut(s) 944, 1367, 1520
HgaI GACGC 1 cut(s) 967
HhaI GCGC 1 cut(s) 1153
Hin1I GRCGYC 2 cut(s) 959, 1151
Hin1II CATG 9 cut(s) 251, 329, 333, 536, 768, 998, 1554, 1794, 1840
Hin6I GCGC 1 cut(s) 1151
HinP1I GCGC 1 cut(s) 1151
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 6 cut(s) 410, 683, 856, 1357, 1412, 1547
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 3 cut(s) 944, 1367, 1520
HphI GGTGA 3 cut(s) 332, 515, 1421
Hpy166II GTNNAC 4 cut(s) 304, 518, 664, 957
Hpy8I GTNNAC 4 cut(s) 304, 518, 664, 957
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 4 cut(s) 1247, 1369, 1612, 1621
HpyCH4III ACNGT 2 cut(s) 320, 1198
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 655, 761, 1163, 1647, 1667
HpyF3I CTNAG 9 cut(s) 231, 800, 999, 1055, 1414, 1462, 1770, 1776, 1782
Hsp92I GRCGYC 2 cut(s) 959, 1151
Hsp92II CATG 9 cut(s) 251, 329, 333, 536, 768, 998, 1554, 1794, 1840
HspAI GCGC 1 cut(s) 1151
KasI GGCGCC 1 cut(s) 1150
KspAI GTTAAC 1 cut(s) 664
Kzo9I GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
LmnI GCTCC 5 cut(s) 26, 77, 274, 761, 1742
Lsp1109I GCAGC 8 cut(s) 77, 110, 967, 999, 1178, 1399, 1842, 1845
LweI GCATC 3 cut(s) 725, 1119, 1422
MaeI CTAG 5 cut(s) 297, 651, 1097, 1667, 1709
MaeIII GTNAC 7 cut(s) 320, 404, 767, 1058, 1327, 1409, 1446
MalI GATC 6 cut(s) 194, 283, 786, 1140, 1632, 1859
MbiI CCGCTC 1 cut(s) 1126
MboI GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
MfeI CAATTG 1 cut(s) 1119
MflI RGATCY 2 cut(s) 281, 1138
MhlI GDGCHC 1 cut(s) 894
MlsI TGGCCA 1 cut(s) 1589
MluNI TGGCCA 1 cut(s) 1589
Mly113I GGCGCC 1 cut(s) 1151
MlyI GAGTC 2 cut(s) 850, 1406
Mox20I TGGCCA 1 cut(s) 1589
Mph1103I ATGCAT 3 cut(s) 331, 1585, 1792
MscI TGGCCA 1 cut(s) 1589
MseI TTAA 7 cut(s) 663, 935, 1008, 1088, 1113, 1493, 1736
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 1589
MspA1I CMGCKG 1 cut(s) 1277
MspI CCGG 3 cut(s) 944, 1367, 1520
MspR9I CCNGG 5 cut(s) 83, 674, 1407, 1591, 1762
MunI CAATTG 1 cut(s) 1119
Mva1269I GAATGC 2 cut(s) 1585, 1792
MvaI CCWGG 5 cut(s) 83, 674, 1407, 1591, 1762
MwoI GCNNNNNNNGC 6 cut(s) 129, 655, 761, 1163, 1647, 1667
NarI GGCGCC 1 cut(s) 1151
NcoI CCATGG 1 cut(s) 247
NdeII GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
NlaIII CATG 9 cut(s) 251, 329, 333, 536, 768, 998, 1554, 1794, 1840
NlaIV GGNNCC 5 cut(s) 22, 73, 209, 1152, 1744
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 4 cut(s) 320, 1058, 1409, 1446
NsiI ATGCAT 3 cut(s) 331, 1585, 1792
NspI RCATGY 4 cut(s) 333, 768, 998, 1794
NspV TTCGAA 1 cut(s) 1270
PaeI GCATGC 3 cut(s) 333, 998, 1794
PaeR7I CTCGAG 1 cut(s) 1451
PasI CCCWGGG 1 cut(s) 673
PcsI WCGNNNNNNNCGW 1 cut(s) 1763
PctI GAATGC 2 cut(s) 1585, 1792
PfeI GAWTC 4 cut(s) 410, 683, 1357, 1547
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 8 cut(s) 92, 125, 982, 1014, 1168, 1389, 1832, 1835
PleI GAGTC 2 cut(s) 850, 1406
PluTI GGCGCC 1 cut(s) 1154
PpsI GAGTC 2 cut(s) 850, 1406
PsiI TTATAA 1 cut(s) 540
Psp6I CCWGG 5 cut(s) 81, 672, 1405, 1589, 1760
PspEI GGTNACC 1 cut(s) 320
PspGI CCWGG 5 cut(s) 81, 672, 1405, 1589, 1760
PspN4I GGNNCC 5 cut(s) 22, 73, 209, 1152, 1744
PspXI VCTCGAGB 1 cut(s) 1451
PstI CTGCAG 3 cut(s) 95, 1159, 1168
PstNI CAGNNNCTG 2 cut(s) 272, 1163
PsuI RGATCY 2 cut(s) 281, 1138
PvuII CAGCTG 1 cut(s) 1277
RsaI GTAC 3 cut(s) 446, 797, 1200
RsaNI GTAC 3 cut(s) 445, 796, 1199
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 7 cut(s) 663, 935, 1008, 1088, 1113, 1493, 1736
SatI GCNGC 8 cut(s) 91, 124, 981, 1013, 1167, 1388, 1831, 1834
Sau3AI GATC 6 cut(s) 192, 281, 784, 1138, 1630, 1857
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 2 cut(s) 850, 1406
ScrFI CCNGG 5 cut(s) 83, 674, 1407, 1591, 1762
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 3 cut(s) 725, 1119, 1422
SfcI CTRYAG 4 cut(s) 91, 1155, 1164, 1861
SfoI GGCGCC 1 cut(s) 1152
Sfr274I CTCGAG 1 cut(s) 1451
SfuI TTCGAA 1 cut(s) 1270
SlaI CTCGAG 1 cut(s) 1451
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 1451
SmoI CTYRAG 1 cut(s) 1451
SpeI ACTAGT 1 cut(s) 1708
SphI GCATGC 3 cut(s) 333, 998, 1794
SsiI CCGC 2 cut(s) 354, 1126
SspDI GGCGCC 1 cut(s) 1150
SspI AATATT 1 cut(s) 1845
SspMI CTAG 5 cut(s) 297, 651, 1097, 1667, 1709
StyD4I CCNGG 5 cut(s) 81, 672, 1405, 1589, 1760
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 320, 1198
TaqI TCGA 5 cut(s) 888, 1270, 1452, 1498, 1644
TaqII GACCGA 1 cut(s) 227
TatI WGTACW 2 cut(s) 444, 1198
TfiI GAWTC 4 cut(s) 410, 683, 1357, 1547
Tru1I TTAA 7 cut(s) 663, 935, 1008, 1088, 1113, 1493, 1736
Tru9I TTAA 7 cut(s) 663, 935, 1008, 1088, 1113, 1493, 1736
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 4 cut(s) 320, 1058, 1409, 1446
TseI GCWGC 8 cut(s) 90, 123, 980, 1012, 1166, 1387, 1830, 1833
Tsp45I GTSAC 4 cut(s) 320, 1058, 1409, 1446
TspDTI ATGAA 4 cut(s) 17, 1266, 1557, 1837
TspGWI ACGGA 4 cut(s) 476, 710, 805, 1410
TspRI CASTG 1 cut(s) 115
XapI RAATTY 6 cut(s) 262, 608, 865, 914, 1401, 1722
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 4 cut(s) 333, 768, 998, 1794
XhoI CTCGAG 1 cut(s) 1451
XspI CTAG 5 cut(s) 297, 651, 1097, 1667, 1709
ZrmI AGTACT 1 cut(s) 446
Zsp2I ATGCAT 3 cut(s) 331, 1585, 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.