RchiOBHm_Chr7g0194211

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013940
Physical Location & Seq
Reverse (-)
12352359 .. 12355627
3269 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17368

Sequence Viewer

Length: 1881 bp
ATGAAGAAGCCTCGCCACGGTTCCTTACATATCAAAATCCAAAGCAACAACAACTCTGACACCAAGGACAGAGGAGACAAAAACAACAAGAGCAGCAACCACTACAACAACAATAAAGGGAAGAGCTTCTATGAGCTCTCTCTGTCTTTGATCTTCTCTCTCTGGTGTCTTGTTGTCCTGTTCTACTCTAAGCTTGGTCTTGGCCATGGCAATGGAGGGAATATTTCAGGTCCGAGTAACAGAACTACACATTGTCCTGGTGTTCACAATGGTAAGCATGCATATTCATTTGTGGCAAATGGAGGCGAAAATAAGACAAATGGGATGCTTTTAGATTATAATTCGTCTGAAAGTTGTAATGGTACTGCTGTTGGTCACAACTTGCCAGTTTCGAAGTACTCACTGCCGGAGACAAATGGATTAGAAAAGATAGTTTGGAGAGTTTTGGGTTATAGCAGTTTGGTCTGTGAACTCAACCAAGAACAAGAACAGCATAACAGAAACTGTCCAGGACAATGTGTGAATAGAACTTCTCACTCTACCTATCTCAATCTTGATGAATTTCGAAACGTAACAAAGCAAGAGAAAGACCGTGTAATGCCTAGTCAGCTTGCTAACATTACCCATGGGTTCGAACCTGATGGAACGGAGTACAACTATGCCTCGGAATCCAAGGGGGCAAAGGTGGTGTCTCACAACAAGGAAGCAAAAGGAGCAAGTAACATATTAGGCAAGGATCACGATAAATACCTCAGAAACCCTTGTTCCGTAGAGGGAAAGTTTGTTATAATTGAGCTCGCAGAGGAGACTCTGGTAGATGCTGTCAAAATTGCAAATTTTGAGCACTACTCTTCTAATTTCAAGGAATTTAAATTGTCTGGCAGTTTAAGCTATCCAACAGAAGCATGGTCACCGCTGGGTAGCTTTGTTGCTGCTAATAGTAAGCATGCTCAAACCTTTAAGCTTCCTGAACCCAAATGGATGAGATACCTAAAGTTGGATTTAGTAAGCCACTATGGAGCAGAGTTTTACTGCACACTTAGTGTTCTAGAGGTGTATGGTATTAATGCAATCGAAAGAATGCTTGAAGATCTCATTGTGGAGTCTGCAGAATCTGCAACCAACAAGTTGCCAGAGCCTAATGCAACTGTAATATCTTCTACAAAAGAAGAGGTTGGTCCAACTGACCGGAAAACAAGCAGCGATAGTCAAAATGGGGTTGGTGCAGCTGTAGGGATACAAAGCACTGAAGAAGCGCAACGAGCCAATTCAGATGCTTCCAAGAATTCAGTAACTACTAACAAGATTCCTGATCCGGTTATGACAGTTCGGCAGCAGCCAAATGGAAGAATTCCTGGTGATTCTGTTCTGAAGATTTTGATGCAGAAGGTGCGGTCACTTGAGCTGAACTTGTCCGTGCTGGAGGAGTTTATCAAAGAATTGAACAGAAGACAAGGCGATGTTTTGCCAGAGCTTGGAAAAGAGATATTAAGAATATCATTGCTGTTGGAGCAAAGCAAAATGGAGATTAAAGATCTCATGCAATGGAAGGAAACTATGGAAAAACAAATGAGTGACCTTGAACTATGGAAAGCTGCTGTCTCGTCTCAATTGAATGCATTGGTCAGGGAAAATAACTTGTTGAGATTAGATTTCGAAAAGGTTGTAAATGATCAAGCCAGTCTCGAAAGCAAAGAGCTTGCTATTTTAGCAGTTAGTCTTTTCTTTTTGTGCTTCGCGATCCTCCAGCTAGTTTCATTTCAAATTTTGACATTTTTCAGAACCTCTCAGCCTGACAACGGATGCAGGAAAAGTAGAGGTTGGGTTTTAGTCATTGTTAGCAGCAGTATGACAATATTCATAACTTTGATATATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

626

Amino Acids

69.85

Weight (kDa)

6.98

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 232 - 354 1.1e-33 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 339, 788
AccB7I CCANNNNNTGG 1 cut(s) 1475
AccII CGCG 1 cut(s) 1739
AciI CCGC 2 cut(s) 914, 1393
AclWI GGATC 3 cut(s) 744, 1307, 1735
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 6 cut(s) 560, 835, 866, 1285, 1350, 1764
AcuI CTGAAG 2 cut(s) 1269, 1391
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 3 cut(s) 364, 398, 653
AfiI CCNNNNNNNGG 4 cut(s) 17, 997, 1475, 1799
AgsI TTSAA 6 cut(s) 862, 1088, 1444, 1583, 1615, 1763
AjnI CCWGG 3 cut(s) 256, 508, 1354
Alw21I GWGCWC 3 cut(s) 138, 798, 846
Alw26I GTCTC 7 cut(s) 69, 404, 696, 800, 1606, 1611, 1688
AlwI GGATC 3 cut(s) 744, 1307, 1735
AlwNI CAGNNNCTG 2 cut(s) 504, 1115
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 8 cut(s) 93, 932, 1200, 1226, 1333, 1336, 1595, 1842
ApoI RAATTY 6 cut(s) 560, 835, 866, 1285, 1350, 1764
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 1258
AspS9I GGNCC 2 cut(s) 230, 1178
AsuHPI GGTGA 2 cut(s) 903, 1370
AsuII TTCGAA 4 cut(s) 392, 565, 633, 1656
AvaII GGWCC 2 cut(s) 230, 1178
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BalI TGGCCA 1 cut(s) 204
BanII GRGCYC 2 cut(s) 138, 798
BbsI GAAGAC 1 cut(s) 1456
Bbv12I GWGCWC 3 cut(s) 138, 798, 846
BbvI GCAGC 8 cut(s) 105, 919, 1212, 1238, 1345, 1348, 1582, 1854
BccI CCATC 1 cut(s) 635
BciT130I CCWGG 3 cut(s) 258, 510, 1356
BciVI GTATCC 1 cut(s) 1230
BclI TGATCA 1 cut(s) 1672
BcoDI GTCTC 7 cut(s) 69, 404, 696, 800, 1606, 1611, 1688
BfaI CTAG 4 cut(s) 603, 1049, 1751, 1879
BfmI CTRYAG 2 cut(s) 1107, 1230
BfuI GTATCC 1 cut(s) 1230
BglII AGATCT 2 cut(s) 1090, 1534
BisI GCNGC 8 cut(s) 94, 933, 1201, 1227, 1334, 1337, 1596, 1843
BlsI GCNGC 8 cut(s) 95, 934, 1202, 1228, 1335, 1338, 1597, 1844
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 258, 510, 1356
Bme18I GGWCC 2 cut(s) 230, 1178
BmgT120I GGNCC 2 cut(s) 230, 1178
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 258, 510, 1356
BmsI GCATC 5 cut(s) 315, 808, 1264, 1371, 1793
BpiI GAAGAC 1 cut(s) 1456
BplI GAGNNNNNCTC 2 cut(s) 833, 865
BpmI CTGGAG 2 cut(s) 1442, 1730
Bpu14I TTCGAA 4 cut(s) 392, 565, 633, 1656
BpuEI CTTGAG 1 cut(s) 1421
BsaJI CCNNGG 6 cut(s) 16, 63, 205, 625, 663, 672
BsaWI WCCGGW 2 cut(s) 1188, 1315
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 997, 1475, 1799
Bse1I ACTGG 2 cut(s) 386, 1680
Bse3DI GCAATG 3 cut(s) 217, 1499, 1550
BseBI CCWGG 3 cut(s) 258, 510, 1356
BseDI CCNNGG 6 cut(s) 16, 63, 205, 625, 663, 672
BseGI GGATG 3 cut(s) 330, 987, 1808
BseLI CCNNNNNNNGG 4 cut(s) 17, 997, 1475, 1799
BseMI GCAATG 3 cut(s) 217, 1499, 1550
BseMII CTCAG 2 cut(s) 766, 1802
BseNI ACTGG 2 cut(s) 386, 1680
BseRI GAGGAG 3 cut(s) 87, 818, 1439
BseXI GCAGC 8 cut(s) 105, 919, 1212, 1238, 1345, 1348, 1582, 1854
BseYI CCCAGC 1 cut(s) 916
BsgI GTGCAG 2 cut(s) 1018, 1245
Bsh1236I CGCG 1 cut(s) 1739
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 138, 798, 846
BsiSI CCGG 3 cut(s) 407, 1189, 1316
BslI CCNNNNNNNGG 4 cut(s) 17, 997, 1475, 1799
BsmAI GTCTC 7 cut(s) 69, 404, 696, 800, 1606, 1611, 1688
BsmBI CGTCTC 1 cut(s) 1611
BsmI GAATGC 2 cut(s) 1086, 1621
BsnI GGCC 1 cut(s) 204
Bsp119I TTCGAA 4 cut(s) 392, 565, 633, 1656
Bsp1286I GDGCHC 3 cut(s) 138, 798, 846
Bsp143I GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
Bsp19I CCATGG 2 cut(s) 205, 625
Bsp68I TCGCGA 1 cut(s) 1739
BspACI CCGC 2 cut(s) 914, 1393
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 765, 1801
BspFNI CGCG 1 cut(s) 1739
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 1111
BspPI GGATC 3 cut(s) 744, 1307, 1735
BspQI GCTCTTC 1 cut(s) 116
BspT104I TTCGAA 4 cut(s) 392, 565, 633, 1656
BsrDI GCAATG 3 cut(s) 217, 1499, 1550
BsrI ACTGG 2 cut(s) 386, 1680
BssECI CCNNGG 6 cut(s) 16, 63, 205, 625, 663, 672
BssMI GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
BssT1I CCWWGG 4 cut(s) 63, 205, 625, 672
Bst2UI CCWGG 3 cut(s) 258, 510, 1356
Bst4CI ACNGT 5 cut(s) 20, 506, 593, 1150, 1327
Bst6I CTCTTC 3 cut(s) 116, 856, 1164
BstAPI GCANNNNNTGC 2 cut(s) 1115, 1390
BstBI TTCGAA 4 cut(s) 392, 565, 633, 1656
BstC8I GCNNGC 5 cut(s) 279, 612, 798, 948, 1701
BstDEI CTNAG 4 cut(s) 189, 752, 1040, 1788
BstDSI CCRYGG 3 cut(s) 16, 205, 625
BstEII GGTNACC 1 cut(s) 909
BstF5I GGATG 3 cut(s) 330, 987, 1808
BstFNI CGCG 1 cut(s) 1739
BstHHI GCGC 1 cut(s) 1258
BstKTI GATC 7 cut(s) 153, 739, 1093, 1315, 1537, 1675, 1743
BstMAI GTCTC 7 cut(s) 69, 404, 696, 800, 1606, 1611, 1688
BstMBI GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
BstMWI GCNNNNNNNGC 8 cut(s) 607, 713, 888, 1115, 1262, 1390, 1510, 1709
BstNI CCWGG 3 cut(s) 258, 510, 1356
BstNSI RCATGY 2 cut(s) 281, 950
BstPI GGTNACC 1 cut(s) 909
BstSCI CCNGG 3 cut(s) 256, 508, 1354
BstSFI CTRYAG 2 cut(s) 1107, 1230
BstUI CGCG 1 cut(s) 1739
BstV1I GCAGC 8 cut(s) 105, 919, 1212, 1238, 1345, 1348, 1582, 1854
BstV2I GAAGAC 1 cut(s) 1456
BstX2I RGATCY 2 cut(s) 1090, 1534
BstXI CCANNNNNNTGG 1 cut(s) 212
BstYI RGATCY 2 cut(s) 1090, 1534
BsuI GTATCC 1 cut(s) 1230
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 3 cut(s) 16, 205, 625
BtgZI GCGATG 1 cut(s) 1473
BtsCI GGATG 3 cut(s) 330, 987, 1808
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 2 cut(s) 401, 1245
BtuMI TCGCGA 1 cut(s) 1739
Cac8I GCNNGC 5 cut(s) 279, 612, 798, 948, 1701
CaiI CAGNNNCTG 2 cut(s) 504, 1115
CfoI GCGC 1 cut(s) 1258
Cfr13I GGNCC 2 cut(s) 230, 1178
Csp6I GTAC 3 cut(s) 363, 397, 652
CviAII CATG 6 cut(s) 206, 278, 626, 906, 947, 1540
CviQI GTAC 3 cut(s) 363, 397, 652
DdeI CTNAG 4 cut(s) 189, 752, 1040, 1788
DpnI GATC 7 cut(s) 152, 738, 1092, 1314, 1536, 1674, 1742
DpnII GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
DraI TTTAAA 1 cut(s) 871
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 3 cut(s) 116, 856, 1164
EarI CTCTTC 3 cut(s) 116, 856, 1164
Ecl136II GAGCTC 2 cut(s) 136, 796
Eco130I CCWWGG 4 cut(s) 63, 205, 625, 672
Eco24I GRGCYC 2 cut(s) 138, 798
Eco47I GGWCC 2 cut(s) 230, 1178
Eco53kI GAGCTC 2 cut(s) 136, 796
Eco57I CTGAAG 2 cut(s) 1269, 1391
Eco91I GGTNACC 1 cut(s) 909
EcoICRI GAGCTC 2 cut(s) 136, 796
EcoO65I GGTNACC 1 cut(s) 909
EcoRI GAATTC 2 cut(s) 1285, 1350
EcoRII CCWGG 3 cut(s) 256, 508, 1354
EcoT14I CCWWGG 4 cut(s) 63, 205, 625, 672
EcoT22I ATGCAT 2 cut(s) 283, 1621
EcoT38I GRGCYC 2 cut(s) 138, 798
ErhI CCWWGG 4 cut(s) 63, 205, 625, 672
Esp3I CGTCTC 1 cut(s) 1611
FaeI CATG 6 cut(s) 209, 281, 629, 909, 950, 1543
FatI CATG 6 cut(s) 205, 277, 625, 905, 946, 1539
FbaI TGATCA 1 cut(s) 1672
Fnu4HI GCNGC 8 cut(s) 94, 933, 1201, 1227, 1334, 1337, 1596, 1843
FokI GGATG 3 cut(s) 337, 994, 1815
FriOI GRGCYC 2 cut(s) 138, 798
Fsp4HI GCNGC 8 cut(s) 94, 933, 1201, 1227, 1334, 1337, 1596, 1843
FspBI CTAG 4 cut(s) 603, 1049, 1751, 1879
GlaI GCGC 1 cut(s) 1257
GluI GCNGC 8 cut(s) 94, 933, 1201, 1227, 1334, 1337, 1596, 1843
GsaI CCCAGC 1 cut(s) 920
GsuI CTGGAG 2 cut(s) 1442, 1730
HaeIII GGCC 1 cut(s) 204
HapII CCGG 3 cut(s) 407, 1189, 1316
HhaI GCGC 1 cut(s) 1258
Hin1II CATG 6 cut(s) 209, 281, 629, 909, 950, 1543
Hin6I GCGC 1 cut(s) 1256
HinP1I GCGC 1 cut(s) 1256
HindIII AAGCTT 2 cut(s) 191, 962
HinfI GANTC 6 cut(s) 668, 808, 1103, 1112, 1306, 1361
HpaII CCGG 3 cut(s) 407, 1189, 1316
HphI GGTGA 2 cut(s) 903, 1370
Hpy166II GTNNAC 2 cut(s) 265, 470
Hpy188I TCNGA 8 cut(s) 58, 234, 349, 667, 755, 1273, 1371, 1781
Hpy188III TCNNGA 7 cut(s) 554, 740, 968, 1049, 1310, 1685, 1738
Hpy8I GTNNAC 2 cut(s) 265, 470
HpyAV CCTTC 2 cut(s) 1381, 1543
HpyCH4III ACNGT 5 cut(s) 20, 506, 593, 1150, 1327
HpyCH4IV ACGT 1 cut(s) 570
HpyF10VI GCNNNNNNNGC 8 cut(s) 607, 713, 888, 1115, 1262, 1390, 1510, 1709
HpyF3I CTNAG 4 cut(s) 189, 752, 1040, 1788
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 6 cut(s) 209, 281, 629, 909, 950, 1543
HspAI GCGC 1 cut(s) 1256
Ksp22I TGATCA 1 cut(s) 1672
Kzo9I GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 3 cut(s) 713, 1019, 1510
Lsp1109I GCAGC 8 cut(s) 105, 919, 1212, 1238, 1345, 1348, 1582, 1854
LweI GCATC 5 cut(s) 315, 808, 1264, 1371, 1793
MaeI CTAG 4 cut(s) 603, 1049, 1751, 1879
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 8 cut(s) 236, 374, 571, 719, 909, 1291, 1395, 1574
MalI GATC 7 cut(s) 152, 738, 1092, 1314, 1536, 1674, 1742
MboI GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
MfeI CAATTG 1 cut(s) 1610
MflI RGATCY 2 cut(s) 1090, 1534
MhlI GDGCHC 3 cut(s) 138, 798, 846
MlsI TGGCCA 1 cut(s) 204
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 2 cut(s) 802, 1112
MmeI TCCRAC 4 cut(s) 920, 978, 1205, 1488
Mox20I TGGCCA 1 cut(s) 204
Mph1103I ATGCAT 2 cut(s) 283, 1621
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 204
MseI TTAA 6 cut(s) 870, 887, 960, 1065, 1490, 1530
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 2 cut(s) 916, 1229
MspI CCGG 3 cut(s) 407, 1189, 1316
MspR9I CCNGG 3 cut(s) 258, 510, 1356
MunI CAATTG 1 cut(s) 1610
Mva1269I GAATGC 2 cut(s) 1086, 1621
MvaI CCWGG 3 cut(s) 258, 510, 1356
MvnI CGCG 1 cut(s) 1739
MwoI GCNNNNNNNGC 8 cut(s) 607, 713, 888, 1115, 1262, 1390, 1510, 1709
NcoI CCATGG 2 cut(s) 205, 625
NdeII GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
NlaIII CATG 6 cut(s) 209, 281, 629, 909, 950, 1543
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 4 cut(s) 374, 909, 1395, 1574
NruI TCGCGA 1 cut(s) 1739
NsiI ATGCAT 2 cut(s) 283, 1621
NspI RCATGY 2 cut(s) 281, 950
NspV TTCGAA 4 cut(s) 392, 565, 633, 1656
PaeI GCATGC 2 cut(s) 281, 950
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 2 cut(s) 1086, 1621
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 4 cut(s) 668, 1112, 1306, 1361
PflMI CCANNNNNTGG 1 cut(s) 1475
PfoI TCCNGGA 1 cut(s) 508
PkrI GCNGC 8 cut(s) 95, 934, 1202, 1228, 1335, 1338, 1597, 1844
PleI GAGTC 2 cut(s) 802, 1111
PpsI GAGTC 2 cut(s) 802, 1111
PshBI ATTAAT 1 cut(s) 1065
PsiI TTATAA 2 cut(s) 339, 788
Psp124BI GAGCTC 2 cut(s) 138, 798
Psp6I CCWGG 3 cut(s) 256, 508, 1354
PspEI GGTNACC 1 cut(s) 909
PspFI CCCAGC 1 cut(s) 916
PspGI CCWGG 3 cut(s) 256, 508, 1354
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 230, 1178
PstI CTGCAG 1 cut(s) 1111
PstNI CAGNNNCTG 2 cut(s) 504, 1115
PsuI RGATCY 2 cut(s) 1090, 1534
PvuII CAGCTG 1 cut(s) 1229
RruI TCGCGA 1 cut(s) 1739
RsaI GTAC 3 cut(s) 364, 398, 653
RsaNI GTAC 3 cut(s) 363, 397, 652
RseI CAYNNNNRTG 1 cut(s) 210
SacI GAGCTC 2 cut(s) 138, 798
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 6 cut(s) 870, 887, 960, 1065, 1490, 1530
SatI GCNGC 8 cut(s) 94, 933, 1201, 1227, 1334, 1337, 1596, 1843
Sau3AI GATC 7 cut(s) 150, 736, 1090, 1312, 1534, 1672, 1740
Sau96I GGNCC 2 cut(s) 230, 1178
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 802, 1112
ScrFI CCNGG 3 cut(s) 258, 510, 1356
SduI GDGCHC 3 cut(s) 138, 798, 846
SfaNI GCATC 5 cut(s) 315, 808, 1264, 1371, 1793
SfcI CTRYAG 2 cut(s) 1107, 1230
SfuI TTCGAA 4 cut(s) 392, 565, 633, 1656
SinI GGWCC 2 cut(s) 230, 1178
SmiI ATTTAAAT 1 cut(s) 871
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 1400
SmoI CTYRAG 1 cut(s) 1400
SphI GCATGC 2 cut(s) 281, 950
SsiI CCGC 2 cut(s) 914, 1393
SspI AATATT 2 cut(s) 223, 1857
SspMI CTAG 4 cut(s) 603, 1049, 1751, 1879
SstI GAGCTC 2 cut(s) 138, 798
StyD4I CCNGG 3 cut(s) 256, 508, 1354
StyI CCWWGG 4 cut(s) 63, 205, 625, 672
SwaI ATTTAAAT 1 cut(s) 871
TaaI ACNGT 5 cut(s) 20, 506, 593, 1150, 1327
TaiI ACGT 1 cut(s) 573
TaqI TCGA 6 cut(s) 392, 565, 633, 1074, 1656, 1686
TatI WGTACW 2 cut(s) 396, 651
TfiI GAWTC 4 cut(s) 668, 1112, 1306, 1361
Tru1I TTAA 6 cut(s) 870, 887, 960, 1065, 1490, 1530
Tru9I TTAA 6 cut(s) 870, 887, 960, 1065, 1490, 1530
TscAI CASTG 2 cut(s) 408, 1252
TseFI GTSAC 4 cut(s) 374, 909, 1395, 1574
TseI GCWGC 8 cut(s) 93, 932, 1200, 1226, 1333, 1336, 1595, 1842
Tsp45I GTSAC 4 cut(s) 374, 909, 1395, 1574
TspDTI ATGAA 5 cut(s) 17, 276, 573, 1746, 1849
TspGWI ACGGA 4 cut(s) 662, 757, 1405, 1815
TspRI CASTG 2 cut(s) 408, 1252
Van91I CCANNNNNTGG 1 cut(s) 1475
VpaK11BI GGWCC 2 cut(s) 230, 1178
VspI ATTAAT 1 cut(s) 1065
XapI RAATTY 6 cut(s) 560, 835, 866, 1285, 1350, 1764
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 2 cut(s) 281, 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 4 cut(s) 603, 1049, 1751, 1879
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 2 cut(s) 283, 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.