Rh7DG143100

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013940
Physical Location & Seq
Reverse (-)
12204593 .. 12208048
3456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG143100.1

Sequence Viewer

Length: 1557 bp
ATGCTTTTAGATTATAATTCGTCTGAAAGTTGTAATGGTACTGCTGTTGGTCACAACTTGCCAGTTTCGAAGTACTCACTGCCGGAGACAAATGGATTAGAAAAGATAGTTTGGAGAGTTTTGGGTTATAGCAGTTTGGTCTGTGAACTCAACCAAGAACAAGAACAGCATAGCAGAAACTGTCCAGGACAATGTGTGAATAGAACTTCTCACTCTACCTATCTCAATCTTGATGAATTTCGAAACGTAACAAAGCAAGAGAAAGACCGTGTAATGCCTAGTCAGCTTGTTAACATTACCCATGGGTTCGAACCTGATGGAACGGAGTACAACTATGCCTCGGAATCCAAGGGGGCAAAGGTGGTGTCTCACAACAAGGAAGCAAAAGGAGCAAGCAACATATTAGGCAAGGATCACGATAAATACCTCAGAAACCCTTGCTCTGTAGAGGGAAAGTTTGTTATAATTGAGCTCGCAGAGGAGACTCTGGTAGATGCTGTCAAAATTGCAAATTTTGAGCACTACTCTTCTAATTTCAAGGAATTTAAATTGTCTGGCAGTTTAAGCTATCCAACAGAAGCATGGTCACCGCTGGGTAGCTTTGTTGCTGCTAATAGTAAGCATGCTCAAACCTTTAAGCTTCCTGAACCCAAATGGGTGAGATACCTAAAGTTGGATTTAGTAAGCCACTACGGAGCAGAGTTTTACTGCACACTTAGTGTTCTAGAGGTGTATGGTATTAATGCAATCGAAAGAATGCTTGAAGATCTCATTGTGGAGTCTGCAGAATCTGCAACCAACAAGTTGCCAGAGCCTAATGCAACTGTAATATCTTCTACAAAAGAAGAGGTTGGTCCAACTGACCGGAAAACAAGCAGCGATAGTCAAAATGGGATTGGTGCAGCTGTAGGGATACAAAGCACTGAAGAAGCGCAACGAGCCAATTCAGATGCTTCCAAGAATTCAGTAACTACTAACAAGATTCCTGATCCGGTTATGACAGTTCGGCAGCAGCCAAATGGAAGAATTCCTGGTGATTCTGTGCTGAAGATTTTGATGCAGAAGGTGCGGTCACTTGAGCTGAACTTGTCCGTGCTGGAGGAGTTTATCAAAGAATTGAACAGAAGACAAGGCGATGTTTTGCCAGAGCTTGGAAAAGAGATATTAAGAATATCATTGCTGTTGGAGCAAAGCAAAATGGAGATTAAAGATCTCATGCAATGGAAGGAAACTATGGAAAAACAAATGAGTGACCTTGAACTATGGAAAGCTGCTGTCTCGTCTCAATTGAATGCATTGGTCAGGGAAAATAACTTGTTGAGATTAGATTTCGAAAAGGTTGTAAATGATCAAGCCAGTCTCGAAAGCAAAGAGCTTGCTATTTTAGCAGTTAGTCTTTTCTTTTTGTGCTTCGCGATCCTCCAGCTAGTTTCATTTCAAATTTTGACATTTTTCAGAACCTCTCAGCCTGACAACGGATGCAGGAAAAGTAGAGGTTGGGTTTTAGTCATTGTTAGCAGCAGTATGACAATATTCATAACTTTGATATATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

518

Amino Acids

58.04

Weight (kDa)

5.59

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 124 - 246 4.8e-34 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 464
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccII CGCG 1 cut(s) 1415
AciI CCGC 2 cut(s) 590, 1069
AclWI GGATC 3 cut(s) 420, 983, 1411
AcsI RAATTY 6 cut(s) 236, 511, 542, 961, 1026, 1440
AcuI CTGAAG 2 cut(s) 945, 1067
AdeI CACNNNGTG 1 cut(s) 719
AfaI GTAC 3 cut(s) 40, 74, 329
AfiI CCNNNNNNNGG 3 cut(s) 673, 1151, 1475
AgsI TTSAA 6 cut(s) 538, 764, 1120, 1259, 1291, 1439
AjnI CCWGG 2 cut(s) 184, 1030
Alw21I GWGCWC 2 cut(s) 474, 522
Alw26I GTCTC 6 cut(s) 80, 372, 476, 1282, 1287, 1364
AlwI GGATC 3 cut(s) 420, 983, 1411
AlwNI CAGNNNCTG 2 cut(s) 180, 791
ApeKI GCWGC 7 cut(s) 608, 876, 902, 1009, 1012, 1271, 1518
ApoI RAATTY 6 cut(s) 236, 511, 542, 961, 1026, 1440
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AseI ATTAAT 1 cut(s) 741
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 1 cut(s) 854
AsuHPI GGTGA 3 cut(s) 579, 670, 1046
AsuII TTCGAA 4 cut(s) 68, 241, 309, 1332
AvaII GGWCC 1 cut(s) 854
BaeI ACNNNNGTAYC 2 cut(s) 30, 63
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 1132
Bbv12I GWGCWC 2 cut(s) 474, 522
BbvI GCAGC 7 cut(s) 595, 888, 914, 1021, 1024, 1258, 1530
BccI CCATC 1 cut(s) 311
BciT130I CCWGG 2 cut(s) 186, 1032
BciVI GTATCC 1 cut(s) 906
BclI TGATCA 1 cut(s) 1348
BcoDI GTCTC 6 cut(s) 80, 372, 476, 1282, 1287, 1364
BfaI CTAG 4 cut(s) 279, 725, 1427, 1555
BfmI CTRYAG 3 cut(s) 444, 783, 906
BfuI GTATCC 1 cut(s) 906
BglII AGATCT 2 cut(s) 766, 1210
BisI GCNGC 7 cut(s) 609, 877, 903, 1010, 1013, 1272, 1519
BlsI GCNGC 7 cut(s) 610, 878, 904, 1011, 1014, 1273, 1520
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 186, 1032
Bme18I GGWCC 1 cut(s) 854
BmgT120I GGNCC 1 cut(s) 854
BmrFI CCNGG 2 cut(s) 186, 1032
BmsI GCATC 4 cut(s) 484, 940, 1047, 1469
BpiI GAAGAC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 509, 541
BpmI CTGGAG 2 cut(s) 1118, 1406
Bpu14I TTCGAA 4 cut(s) 68, 241, 309, 1332
BpuEI CTTGAG 1 cut(s) 1097
BsaJI CCNNGG 3 cut(s) 301, 339, 348
BsaWI WCCGGW 2 cut(s) 864, 991
Bsc4I CCNNNNNNNGG 3 cut(s) 673, 1151, 1475
Bse1I ACTGG 2 cut(s) 62, 1356
Bse3DI GCAATG 2 cut(s) 1175, 1226
BseBI CCWGG 2 cut(s) 186, 1032
BseDI CCNNGG 3 cut(s) 301, 339, 348
BseGI GGATG 1 cut(s) 1484
BseLI CCNNNNNNNGG 3 cut(s) 673, 1151, 1475
BseMI GCAATG 2 cut(s) 1175, 1226
BseMII CTCAG 2 cut(s) 442, 1478
BseNI ACTGG 2 cut(s) 62, 1356
BseRI GAGGAG 2 cut(s) 494, 1115
BseXI GCAGC 7 cut(s) 595, 888, 914, 1021, 1024, 1258, 1530
BseYI CCCAGC 1 cut(s) 592
BsgI GTGCAG 2 cut(s) 694, 921
Bsh1236I CGCG 1 cut(s) 1415
BsiHKAI GWGCWC 2 cut(s) 474, 522
BsiSI CCGG 3 cut(s) 83, 865, 992
BslI CCNNNNNNNGG 3 cut(s) 673, 1151, 1475
BsmAI GTCTC 6 cut(s) 80, 372, 476, 1282, 1287, 1364
BsmBI CGTCTC 1 cut(s) 1287
BsmI GAATGC 2 cut(s) 762, 1297
Bsp119I TTCGAA 4 cut(s) 68, 241, 309, 1332
Bsp1286I GDGCHC 2 cut(s) 474, 522
Bsp143I GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
Bsp19I CCATGG 1 cut(s) 301
Bsp68I TCGCGA 1 cut(s) 1415
BspACI CCGC 2 cut(s) 590, 1069
BspCNI CTCAG 2 cut(s) 441, 1477
BspFNI CGCG 1 cut(s) 1415
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 3 cut(s) 420, 983, 1411
BspT104I TTCGAA 4 cut(s) 68, 241, 309, 1332
BsrDI GCAATG 2 cut(s) 1175, 1226
BsrI ACTGG 2 cut(s) 62, 1356
BssECI CCNNGG 3 cut(s) 301, 339, 348
BssMI GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
BssT1I CCWWGG 2 cut(s) 301, 348
Bst2UI CCWGG 2 cut(s) 186, 1032
Bst4CI ACNGT 4 cut(s) 182, 269, 826, 1003
Bst6I CTCTTC 2 cut(s) 532, 840
BstAPI GCANNNNNTGC 2 cut(s) 791, 1066
BstBI TTCGAA 4 cut(s) 68, 241, 309, 1332
BstC8I GCNNGC 4 cut(s) 394, 474, 624, 1377
BstDEI CTNAG 3 cut(s) 428, 716, 1464
BstDSI CCRYGG 1 cut(s) 301
BstEII GGTNACC 1 cut(s) 585
BstF5I GGATG 1 cut(s) 1484
BstFNI CGCG 1 cut(s) 1415
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 6 cut(s) 415, 769, 991, 1213, 1351, 1419
BstMAI GTCTC 6 cut(s) 80, 372, 476, 1282, 1287, 1364
BstMBI GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
BstMWI GCNNNNNNNGC 8 cut(s) 283, 389, 564, 791, 938, 1066, 1186, 1385
BstNI CCWGG 2 cut(s) 186, 1032
BstNSI RCATGY 1 cut(s) 626
BstPI GGTNACC 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 184, 1030
BstSFI CTRYAG 3 cut(s) 444, 783, 906
BstUI CGCG 1 cut(s) 1415
BstV1I GCAGC 7 cut(s) 595, 888, 914, 1021, 1024, 1258, 1530
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 2 cut(s) 766, 1210
BstYI RGATCY 2 cut(s) 766, 1210
BsuI GTATCC 1 cut(s) 906
BtgI CCRYGG 1 cut(s) 301
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 1 cut(s) 1484
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 2 cut(s) 77, 921
BtuMI TCGCGA 1 cut(s) 1415
Cac8I GCNNGC 4 cut(s) 394, 474, 624, 1377
CaiI CAGNNNCTG 2 cut(s) 180, 791
CfoI GCGC 1 cut(s) 934
Cfr13I GGNCC 1 cut(s) 854
Csp6I GTAC 3 cut(s) 39, 73, 328
CviAII CATG 4 cut(s) 302, 582, 623, 1216
CviQI GTAC 3 cut(s) 39, 73, 328
DdeI CTNAG 3 cut(s) 428, 716, 1464
DpnI GATC 6 cut(s) 414, 768, 990, 1212, 1350, 1418
DpnII GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
DraI TTTAAA 1 cut(s) 547
DraIII CACNNNGTG 1 cut(s) 719
Eam1104I CTCTTC 2 cut(s) 532, 840
EarI CTCTTC 2 cut(s) 532, 840
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 2 cut(s) 301, 348
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 854
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 2 cut(s) 945, 1067
Eco91I GGTNACC 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 472
EcoO65I GGTNACC 1 cut(s) 585
EcoRI GAATTC 2 cut(s) 961, 1026
EcoRII CCWGG 2 cut(s) 184, 1030
EcoT14I CCWWGG 2 cut(s) 301, 348
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 2 cut(s) 301, 348
Esp3I CGTCTC 1 cut(s) 1287
FaeI CATG 4 cut(s) 305, 585, 626, 1219
FatI CATG 4 cut(s) 301, 581, 622, 1215
FbaI TGATCA 1 cut(s) 1348
Fnu4HI GCNGC 7 cut(s) 609, 877, 903, 1010, 1013, 1272, 1519
FokI GGATG 1 cut(s) 1491
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 7 cut(s) 609, 877, 903, 1010, 1013, 1272, 1519
FspBI CTAG 4 cut(s) 279, 725, 1427, 1555
GlaI GCGC 1 cut(s) 933
GluI GCNGC 7 cut(s) 609, 877, 903, 1010, 1013, 1272, 1519
GsaI CCCAGC 1 cut(s) 596
GsuI CTGGAG 2 cut(s) 1118, 1406
HapII CCGG 3 cut(s) 83, 865, 992
HhaI GCGC 1 cut(s) 934
Hin1II CATG 4 cut(s) 305, 585, 626, 1219
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 6 cut(s) 344, 484, 779, 788, 982, 1037
HpaI GTTAAC 1 cut(s) 292
HpaII CCGG 3 cut(s) 83, 865, 992
HphI GGTGA 3 cut(s) 579, 670, 1046
Hpy166II GTNNAC 2 cut(s) 146, 292
Hpy188I TCNGA 5 cut(s) 25, 343, 431, 949, 1457
Hpy188III TCNNGA 7 cut(s) 230, 416, 644, 725, 986, 1361, 1414
Hpy8I GTNNAC 2 cut(s) 146, 292
HpyAV CCTTC 2 cut(s) 1057, 1219
HpyCH4III ACNGT 4 cut(s) 182, 269, 826, 1003
HpyCH4IV ACGT 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 8 cut(s) 283, 389, 564, 791, 938, 1066, 1186, 1385
HpyF3I CTNAG 3 cut(s) 428, 716, 1464
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 4 cut(s) 305, 585, 626, 1219
HspAI GCGC 1 cut(s) 932
Ksp22I TGATCA 1 cut(s) 1348
KspAI GTTAAC 1 cut(s) 292
Kzo9I GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
LmnI GCTCC 3 cut(s) 389, 695, 1186
Lsp1109I GCAGC 7 cut(s) 595, 888, 914, 1021, 1024, 1258, 1530
LweI GCATC 4 cut(s) 484, 940, 1047, 1469
MaeI CTAG 4 cut(s) 279, 725, 1427, 1555
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 6 cut(s) 50, 247, 585, 967, 1071, 1250
MalI GATC 6 cut(s) 414, 768, 990, 1212, 1350, 1418
MboI GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
MboII GAAGA 8 cut(s) 519, 776, 825, 857, 938, 1035, 1060, 1137
MfeI CAATTG 1 cut(s) 1286
MflI RGATCY 2 cut(s) 766, 1210
MhlI GDGCHC 2 cut(s) 474, 522
MlyI GAGTC 2 cut(s) 478, 788
MmeI TCCRAC 4 cut(s) 596, 654, 881, 1164
Mph1103I ATGCAT 1 cut(s) 1297
MseI TTAA 7 cut(s) 291, 546, 563, 636, 741, 1166, 1206
MspA1I CMGCKG 2 cut(s) 592, 905
MspI CCGG 3 cut(s) 83, 865, 992
MspR9I CCNGG 2 cut(s) 186, 1032
MunI CAATTG 1 cut(s) 1286
Mva1269I GAATGC 2 cut(s) 762, 1297
MvaI CCWGG 2 cut(s) 186, 1032
MvnI CGCG 1 cut(s) 1415
MwoI GCNNNNNNNGC 8 cut(s) 283, 389, 564, 791, 938, 1066, 1186, 1385
NcoI CCATGG 1 cut(s) 301
NdeII GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
NlaIII CATG 4 cut(s) 305, 585, 626, 1219
NmuCI GTSAC 4 cut(s) 50, 585, 1071, 1250
NruI TCGCGA 1 cut(s) 1415
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 626
NspV TTCGAA 4 cut(s) 68, 241, 309, 1332
PaeI GCATGC 1 cut(s) 626
PctI GAATGC 2 cut(s) 762, 1297
PfeI GAWTC 4 cut(s) 344, 788, 982, 1037
PflMI CCANNNNNTGG 1 cut(s) 1151
PfoI TCCNGGA 1 cut(s) 184
PkrI GCNGC 7 cut(s) 610, 878, 904, 1011, 1014, 1273, 1520
PleI GAGTC 2 cut(s) 478, 787
PpsI GAGTC 2 cut(s) 478, 787
PshBI ATTAAT 1 cut(s) 741
PsiI TTATAA 2 cut(s) 15, 464
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 184, 1030
PspEI GGTNACC 1 cut(s) 585
PspFI CCCAGC 1 cut(s) 592
PspGI CCWGG 2 cut(s) 184, 1030
PspPI GGNCC 1 cut(s) 854
PstI CTGCAG 1 cut(s) 787
PstNI CAGNNNCTG 2 cut(s) 180, 791
PsuI RGATCY 2 cut(s) 766, 1210
PvuII CAGCTG 1 cut(s) 905
RruI TCGCGA 1 cut(s) 1415
RsaI GTAC 3 cut(s) 40, 74, 329
RsaNI GTAC 3 cut(s) 39, 73, 328
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 7 cut(s) 291, 546, 563, 636, 741, 1166, 1206
SatI GCNGC 7 cut(s) 609, 877, 903, 1010, 1013, 1272, 1519
Sau3AI GATC 6 cut(s) 412, 766, 988, 1210, 1348, 1416
Sau96I GGNCC 1 cut(s) 854
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 478, 788
ScrFI CCNGG 2 cut(s) 186, 1032
SduI GDGCHC 2 cut(s) 474, 522
SfaNI GCATC 4 cut(s) 484, 940, 1047, 1469
SfcI CTRYAG 3 cut(s) 444, 783, 906
SfuI TTCGAA 4 cut(s) 68, 241, 309, 1332
SinI GGWCC 1 cut(s) 854
SmiI ATTTAAAT 1 cut(s) 547
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SphI GCATGC 1 cut(s) 626
SsiI CCGC 2 cut(s) 590, 1069
SspI AATATT 1 cut(s) 1533
SspMI CTAG 4 cut(s) 279, 725, 1427, 1555
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 2 cut(s) 184, 1030
StyI CCWWGG 2 cut(s) 301, 348
SwaI ATTTAAAT 1 cut(s) 547
TaaI ACNGT 4 cut(s) 182, 269, 826, 1003
TaiI ACGT 1 cut(s) 249
TaqI TCGA 6 cut(s) 68, 241, 309, 750, 1332, 1362
TatI WGTACW 2 cut(s) 72, 327
TfiI GAWTC 4 cut(s) 344, 788, 982, 1037
Tru1I TTAA 7 cut(s) 291, 546, 563, 636, 741, 1166, 1206
Tru9I TTAA 7 cut(s) 291, 546, 563, 636, 741, 1166, 1206
TscAI CASTG 2 cut(s) 84, 928
TseFI GTSAC 4 cut(s) 50, 585, 1071, 1250
TseI GCWGC 7 cut(s) 608, 876, 902, 1009, 1012, 1271, 1518
Tsp45I GTSAC 4 cut(s) 50, 585, 1071, 1250
TspDTI ATGAA 3 cut(s) 249, 1422, 1525
TspGWI ACGGA 4 cut(s) 338, 708, 1081, 1491
TspRI CASTG 2 cut(s) 84, 928
Van91I CCANNNNNTGG 1 cut(s) 1151
VpaK11BI GGWCC 1 cut(s) 854
VspI ATTAAT 1 cut(s) 741
XapI RAATTY 6 cut(s) 236, 511, 542, 961, 1026, 1440
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XspI CTAG 4 cut(s) 279, 725, 1427, 1555
ZrmI AGTACT 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.