AT4G25800

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004372
Physical Location & Seq
Forward (+)
13124898 .. 13128045
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25800.2

Sequence Viewer

Length: 1806 bp
ATGAAGAGGAATTTCGAGAGGAACGATGACGACAAGCCTGAGCGAAAGCGTCCTGCTCTTGCTAGTGTGATTGTTGAGGCTCTGAAAGTTGACAGTTTGCAGAAGCTTTGCTCGTCTTTGGAACCTATCCTCCGCCGAGTTGTTAGTGAGGAGGTGGAACGGGCTTTAGCAAAATTAGTCCCTACTAGGCTTACTACAAGTTCTGTATTTTCTCCGAAACGAATTGGAGGTCCAGATGGCCGGAACTTGCAGCTGCATTTCAAGTCTAGATTATCTCTCCCTTTATTCACTGGAGGAAGAGTGGAAGGAGAGCAAGGTGCTACTATCCATGTTGTATTGATTGATGCAAACACGGGTCGTCCTGTAACAGTTGGACCAGAAGCTTCCCTGAAGCTTGAGGTTGTTGTTCTTGGGGGTGACTTTAACAATGAAGATGATGAAGATTGGACCCAGGAAGAGTTTGAAAGTCATGTTGTAAAAGAGCGTGAAGGAAAGAGACCTTTGCTTACTGGAGACTTGTTTGTTGTTCTCAAGGAAGGGGTAGGCACTTTGGGTGAGATTGTTTTCACGGATAATTCGAGTTGGATTAGAAGTCGCAAGTTCAGGCTTGGTTTGAGGGTACCCTCTGGGTACTGTGATGGCATACGTATACGTGAAGCAAAAACAGAAGCTTTCTCTGTTAAAGATCACAGGGGCGAATTGTACAAGAAGCATTATCCACCTGCTTTGAATGATGAGGTGTGGAGGTTAGAGAAGATTGGTAAAGATGGGGCGTTTCATAAGAGGCTAACCGCTGCAGGAATAGTCACTGTGGAAGGCTTTCTGAGACAATTGGTCAGAGATTCTACAAAATTACGGGCTATTCTTGGAAGTGGCATGTCAAATAAGATGTGGGATTTGCTAGTGGAGCATGCAAAAACATGTGTCCTAAGCGGTAAGCTTTATATCTACTATACTGAAGATTCAAGGTCTGTTGGCGTTGTATTCAATAACATCTACGAGTTAAGTGGCCTCATAACTGAAGACCAATACTTATCTGCTGATTCACTCTCTGAAAGCCAAAAGGTATATGTGGATGGACTGGTGAAGAAAGCGTATGAAAATTGGAACCAGGTCGTGGAGTATGAAGGCGAATCTCTTTTAAACTTAAACCAGCCTGAGAGGTTGGATATTTCTCAGACTGATCCTGTAACAGCTCTGGCAAGTTACTCTACGGTGCCTCTTAGTCAGTTTCCTGAATTCGCTATAGAAGGGTATAATCAAACGCTGACAACAGCCCTACCTCATAACCCACAAGCACAATTTGATTTTGTTCCCCAGCAAGATCAGTTTATCGGAATACAGCAACCTCAAACTCAAACCAACATAGAGAATGAAAATGTAACTAGGTTGGTTCTTGGTCCTCCACAATCATCCACAGGTGGGTACCAAGACATCAAATCTTCTGCTGACCAGGAAAATCTTAATCCTTTCGAAGACTGGACAAACCTAAGTGAGAATGACTTTTTCTCGGAGGAAGAGATCCGGCAAACAAGCCATGACTTGTTAGCGAATGAGGACATGCAACAGTTGCTCTTCAGCATGGGAGGTGGCAAAGGAGAAGATGGTTTTACTTTCCCATCGTTTATGCAGAATACACCAATGATGCAGGGATATGATGAGGAGGGCCGTGGGCGATCAGGAAAAGCGGTTGTTGGATGGCTGAAGGTAAAAGCTGCTATGAGATGGGGATTCTTCATAAGGAGGAAAGCTGCTGAGAGGAGAGCACAGATTGTGGAGCTTCATGATAATAACGATGGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

601

Amino Acids

67.59

Weight (kDa)

5.33

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 83 - 230 5.9e-62 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 242 - 308 4e-31 Calmodulin binding protein central domain
Calmod_bind_C PF20452 313 - 375 4.9e-25 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 730
Acc36I ACCTGC 1 cut(s) 730
Acc65I GGTACC 2 cut(s) 619, 1425
AccB1I GGYRCC 3 cut(s) 619, 1216, 1425
AccB7I CCANNNNNTGG 1 cut(s) 1117
AccI GTMKAC 1 cut(s) 649
AciI CCGC 4 cut(s) 133, 792, 933, 1688
AclWI GGATC 2 cut(s) 1178, 1516
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 2 cut(s) 10, 1238
AcuI CTGAAG 5 cut(s) 410, 978, 1041, 1561, 1724
AfaI GTAC 4 cut(s) 621, 632, 704, 1427
AfiI CCNNNNNNNGG 2 cut(s) 1117, 1422
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 5 cut(s) 262, 464, 730, 966, 988
AhdI GACNNNNNGTC 1 cut(s) 833
AjnI CCWGG 3 cut(s) 450, 1110, 1452
AloI GAACNNNNNNTCC 2 cut(s) 114, 146
Alw21I GWGCWC 1 cut(s) 1768
Alw26I GTCTC 3 cut(s) 490, 507, 820
AlwI GGATC 2 cut(s) 1178, 1516
AoxI GGCC 3 cut(s) 238, 1009, 1666
ApeKI GCWGC 5 cut(s) 250, 253, 794, 1715, 1751
ApoI RAATTY 2 cut(s) 10, 1238
ArsI GACNNNNNNTTYG 2 cut(s) 214, 246
Asp700I GAANNNNTTC 1 cut(s) 819
Asp718I GGTACC 2 cut(s) 619, 1425
AspS9I GGNCC 5 cut(s) 230, 374, 447, 1400, 1666
AsuHPI GGTGA 3 cut(s) 428, 566, 1096
AsuII TTCGAA 1 cut(s) 1473
AvaII GGWCC 4 cut(s) 230, 374, 447, 1400
BanI GGYRCC 3 cut(s) 619, 1216, 1425
BbsI GAAGAC 2 cut(s) 1029, 1482
Bbv12I GWGCWC 1 cut(s) 1768
BbvI GCAGC 5 cut(s) 240, 262, 781, 1702, 1738
BccI CCATC 9 cut(s) 230, 632, 761, 1070, 1598, 1627, 1692, 1719, 1790
BceAI ACGGC 1 cut(s) 1653
BciT130I CCWGG 3 cut(s) 452, 1112, 1454
BcoDI GTCTC 3 cut(s) 490, 507, 820
BfaI CTAG 5 cut(s) 63, 186, 267, 902, 1386
BfmI CTRYAG 2 cut(s) 795, 1245
BfuAI ACCTGC 1 cut(s) 730
BisI GCNGC 5 cut(s) 251, 254, 795, 1716, 1752
BlsI GCNGC 5 cut(s) 252, 255, 796, 1717, 1753
Bme1390I CCNGG 3 cut(s) 452, 1112, 1454
Bme18I GGWCC 4 cut(s) 230, 374, 447, 1400
BmeRI GACNNNNNGTC 1 cut(s) 833
BmgT120I GGNCC 5 cut(s) 230, 374, 447, 1400, 1666
BmiI GGNNCC 6 cut(s) 123, 449, 621, 1109, 1218, 1427
BmrFI CCNGG 3 cut(s) 452, 1112, 1454
BmsI GCATC 2 cut(s) 334, 1635
BpiI GAAGAC 2 cut(s) 1029, 1482
BpmI CTGGAG 2 cut(s) 312, 531
Bpu10I CCTNAGC 2 cut(s) 39, 929
Bpu14I TTCGAA 1 cut(s) 1473
BpuEI CTTGAG 2 cut(s) 416, 515
BsaAI YACGTR 2 cut(s) 647, 653
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 450, 1669
BsaXI ACNNNNNCTCC 4 cut(s) 114, 144, 285, 315
Bsc4I CCNNNNNNNGG 2 cut(s) 1117, 1422
Bse1I ACTGG 4 cut(s) 295, 514, 1086, 1484
BseBI CCWGG 3 cut(s) 452, 1112, 1454
BseDI CCNNGG 2 cut(s) 450, 1669
BseGI GGATG 3 cut(s) 1081, 1412, 1703
BseLI CCNNNNNNNGG 2 cut(s) 1117, 1422
BseMII CTCAG 5 cut(s) 30, 815, 1149, 1190, 1746
BseNI ACTGG 4 cut(s) 295, 514, 1086, 1484
BseRI GAGGAG 3 cut(s) 164, 1676, 1774
BseXI GCAGC 5 cut(s) 240, 262, 781, 1702, 1738
BseYI CCCAGC 1 cut(s) 1317
BshFI GGCC 3 cut(s) 240, 1011, 1668
BshNI GGYRCC 3 cut(s) 619, 1216, 1425
BsiHKAI GWGCWC 1 cut(s) 1768
BsiSI CCGG 2 cut(s) 241, 1525
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 2 cut(s) 1117, 1422
BsmAI GTCTC 3 cut(s) 490, 507, 820
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 3 cut(s) 240, 1011, 1668
Bso31I GGTCTC 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 1473
Bsp1286I GDGCHC 1 cut(s) 1768
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
BspACI CCGC 4 cut(s) 133, 792, 933, 1688
BspANI GGCC 3 cut(s) 240, 1011, 1668
BspCNI CTCAG 5 cut(s) 31, 816, 1150, 1189, 1747
BspHI TCATGA 1 cut(s) 1783
BspLI GGNNCC 6 cut(s) 123, 449, 621, 1109, 1218, 1427
BspMAI CTGCAG 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 730
BspPI GGATC 2 cut(s) 1178, 1516
BspQI GCTCTTC 1 cut(s) 1580
BspT104I TTCGAA 1 cut(s) 1473
BspT107I GGYRCC 3 cut(s) 619, 1216, 1425
BspTNI GGTCTC 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 4 cut(s) 295, 514, 1086, 1484
BssECI CCNNGG 2 cut(s) 450, 1669
BssMI GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
BssNAI GTATAC 1 cut(s) 650
Bst1107I GTATAC 1 cut(s) 650
Bst2UI CCWGG 3 cut(s) 452, 1112, 1454
Bst4CI ACNGT 6 cut(s) 95, 370, 635, 811, 1216, 1569
Bst6I CTCTTC 4 cut(s) 292, 450, 1512, 1580
BstAPI GCANNNNNTGC 1 cut(s) 1570
BstAUI TGTACA 1 cut(s) 702
BstBAI YACGTR 2 cut(s) 647, 653
BstBI TTCGAA 1 cut(s) 1473
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 8 cut(s) 39, 824, 929, 1158, 1176, 1223, 1490, 1755
BstDSI CCRYGG 1 cut(s) 1669
BstF5I GGATG 3 cut(s) 1081, 1412, 1703
BstKTI GATC 5 cut(s) 688, 1186, 1327, 1524, 1679
BstMAI GTCTC 3 cut(s) 490, 507, 820
BstMBI GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
BstMWI GCNNNNNNNGC 2 cut(s) 907, 1570
BstNI CCWGG 3 cut(s) 452, 1112, 1454
BstNSI RCATGY 4 cut(s) 880, 914, 924, 1564
BstSCI CCNGG 3 cut(s) 450, 1110, 1452
BstSFI CTRYAG 2 cut(s) 795, 1245
BstSNI TACGTA 1 cut(s) 647
BstV1I GCAGC 5 cut(s) 240, 262, 781, 1702, 1738
BstV2I GAAGAC 2 cut(s) 1029, 1482
BstX2I RGATCY 1 cut(s) 1521
BstYI RGATCY 1 cut(s) 1521
BstZ17I GTATAC 1 cut(s) 650
BsuRI GGCC 3 cut(s) 240, 1011, 1668
BtgI CCRYGG 1 cut(s) 1669
BtsCI GGATG 3 cut(s) 1081, 1412, 1703
BtsIMutI CAGTG 2 cut(s) 288, 807
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 1 cut(s) 912
CciI TCATGA 1 cut(s) 1783
Cfr13I GGNCC 5 cut(s) 230, 374, 447, 1400, 1666
CseI GACGC 1 cut(s) 38
CsiI ACCWGGT 1 cut(s) 1110
Csp6I GTAC 4 cut(s) 620, 631, 703, 1426
CspCI CAANNNNNGTGG 2 cut(s) 708, 743
CviAII CATG 9 cut(s) 329, 470, 877, 911, 921, 1538, 1561, 1582, 1784
CviQI GTAC 4 cut(s) 620, 631, 703, 1426
DdeI CTNAG 8 cut(s) 39, 824, 929, 1158, 1176, 1223, 1490, 1755
DpnI GATC 5 cut(s) 687, 1185, 1326, 1523, 1678
DpnII GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
DraI TTTAAA 1 cut(s) 1143
DriI GACNNNNNGTC 1 cut(s) 833
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 4 cut(s) 292, 450, 1512, 1580
Eam1105I GACNNNNNGTC 1 cut(s) 833
EarI CTCTTC 4 cut(s) 292, 450, 1512, 1580
EciI GGCGGA 1 cut(s) 122
Eco105I TACGTA 1 cut(s) 647
Eco31I GGTCTC 1 cut(s) 490
Eco47I GGWCC 4 cut(s) 230, 374, 447, 1400
Eco57I CTGAAG 5 cut(s) 410, 978, 1041, 1561, 1724
EcoRI GAATTC 1 cut(s) 1238
EcoRII CCWGG 3 cut(s) 450, 1110, 1452
FaeI CATG 9 cut(s) 332, 473, 880, 914, 924, 1541, 1564, 1585, 1787
FaqI GGGAC 1 cut(s) 164
FatI CATG 9 cut(s) 328, 469, 876, 910, 920, 1537, 1560, 1581, 1783
FblI GTMKAC 1 cut(s) 649
Fnu4HI GCNGC 5 cut(s) 251, 254, 795, 1716, 1752
FokI GGATG 3 cut(s) 1088, 1399, 1710
Fsp4HI GCNGC 5 cut(s) 251, 254, 795, 1716, 1752
FspBI CTAG 5 cut(s) 63, 186, 267, 902, 1386
GluI GCNGC 5 cut(s) 251, 254, 795, 1716, 1752
GsaI CCCAGC 1 cut(s) 1321
GsuI CTGGAG 2 cut(s) 312, 531
HaeIII GGCC 3 cut(s) 240, 1011, 1668
HapII CCGG 2 cut(s) 241, 1525
HgaI GACGC 1 cut(s) 38
Hin1II CATG 9 cut(s) 332, 473, 880, 914, 924, 1541, 1564, 1585, 1787
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 5 cut(s) 104, 381, 392, 669, 938
HinfI GANTC 5 cut(s) 842, 962, 1043, 1133, 1731
HpaII CCGG 2 cut(s) 241, 1525
HphI GGTGA 3 cut(s) 428, 566, 1096
Hpy166II GTNNAC 2 cut(s) 91, 650
Hpy188I TCNGA 8 cut(s) 84, 216, 825, 839, 1054, 1179, 1337, 1513
Hpy188III TCNNGA 6 cut(s) 16, 233, 267, 1235, 1680, 1784
Hpy8I GTNNAC 2 cut(s) 91, 650
HpyAV CCTTC 7 cut(s) 299, 482, 530, 809, 1121, 1244, 1699
HpyCH4III ACNGT 6 cut(s) 95, 370, 635, 811, 1216, 1569
HpyCH4IV ACGT 2 cut(s) 646, 652
HpyCH4V TGCA 9 cut(s) 100, 250, 256, 347, 797, 914, 1564, 1630, 1648
HpyF10VI GCNNNNNNNGC 2 cut(s) 907, 1570
HpyF3I CTNAG 8 cut(s) 39, 824, 929, 1158, 1176, 1223, 1490, 1755
HpySE526I ACGT 2 cut(s) 646, 652
Hsp92II CATG 9 cut(s) 332, 473, 880, 914, 924, 1541, 1564, 1585, 1787
KpnI GGTACC 2 cut(s) 623, 1429
Kzo9I GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
LguI GCTCTTC 1 cut(s) 1580
LmnI GCTCC 2 cut(s) 907, 1777
Lsp1109I GCAGC 5 cut(s) 240, 262, 781, 1702, 1738
LweI GCATC 2 cut(s) 334, 1635
MabI ACCWGGT 1 cut(s) 1110
MaeI CTAG 5 cut(s) 63, 186, 267, 902, 1386
MaeII ACGT 2 cut(s) 646, 652
MaeIII GTNAC 6 cut(s) 364, 416, 805, 1189, 1205, 1381
MalI GATC 5 cut(s) 687, 1185, 1326, 1523, 1678
MboI GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 1 cut(s) 1521
MhlI GDGCHC 1 cut(s) 1768
MmeI TCCRAC 4 cut(s) 352, 563, 1146, 1675
MroXI GAANNNNTTC 1 cut(s) 819
MseI TTAA 6 cut(s) 423, 681, 1004, 1142, 1148, 1464
MspA1I CMGCKG 2 cut(s) 253, 794
MspI CCGG 2 cut(s) 241, 1525
MspR9I CCNGG 3 cut(s) 452, 1112, 1454
MunI CAATTG 1 cut(s) 830
MvaI CCWGG 3 cut(s) 452, 1112, 1454
MwoI GCNNNNNNNGC 2 cut(s) 907, 1570
NdeII GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
NlaIII CATG 9 cut(s) 332, 473, 880, 914, 924, 1541, 1564, 1585, 1787
NlaIV GGNNCC 6 cut(s) 123, 449, 621, 1109, 1218, 1427
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 2 cut(s) 416, 805
NspI RCATGY 4 cut(s) 880, 914, 924, 1564
NspV TTCGAA 1 cut(s) 1473
PaeI GCATGC 1 cut(s) 914
PagI TCATGA 1 cut(s) 1783
PaqCI CACCTGC 1 cut(s) 730
PciI ACATGT 1 cut(s) 920
PciSI GCTCTTC 1 cut(s) 1580
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 575
PdmI GAANNNNTTC 1 cut(s) 819
PfeI GAWTC 5 cut(s) 842, 962, 1043, 1133, 1731
PflMI CCANNNNNTGG 1 cut(s) 1117
PkrI GCNGC 5 cut(s) 252, 255, 796, 1717, 1753
Ppu21I YACGTR 2 cut(s) 647, 653
PscI ACATGT 1 cut(s) 920
Psp6I CCWGG 3 cut(s) 450, 1110, 1452
PspFI CCCAGC 1 cut(s) 1317
PspGI CCWGG 3 cut(s) 450, 1110, 1452
PspN4I GGNNCC 6 cut(s) 123, 449, 621, 1109, 1218, 1427
PspPI GGNCC 5 cut(s) 230, 374, 447, 1400, 1666
PstI CTGCAG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 1521
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 4 cut(s) 621, 632, 704, 1427
RsaNI GTAC 4 cut(s) 620, 631, 703, 1426
SapI GCTCTTC 1 cut(s) 1580
SaqAI TTAA 6 cut(s) 423, 681, 1004, 1142, 1148, 1464
SatI GCNGC 5 cut(s) 251, 254, 795, 1716, 1752
Sau3AI GATC 5 cut(s) 685, 1183, 1324, 1521, 1676
Sau96I GGNCC 5 cut(s) 230, 374, 447, 1400, 1666
ScrFI CCNGG 3 cut(s) 452, 1112, 1454
SduI GDGCHC 1 cut(s) 1768
SexAI ACCWGGT 1 cut(s) 1110
SfaNI GCATC 2 cut(s) 334, 1635
SfcI CTRYAG 2 cut(s) 795, 1245
SfuI TTCGAA 1 cut(s) 1473
SinI GGWCC 4 cut(s) 230, 374, 447, 1400
SmlI CTYRAG 2 cut(s) 395, 530
SmoI CTYRAG 2 cut(s) 395, 530
SnaBI TACGTA 1 cut(s) 647
SphI GCATGC 1 cut(s) 914
SsiI CCGC 4 cut(s) 133, 792, 933, 1688
SspMI CTAG 5 cut(s) 63, 186, 267, 902, 1386
StyD4I CCNGG 3 cut(s) 450, 1110, 1452
TaaI ACNGT 6 cut(s) 95, 370, 635, 811, 1216, 1569
TaiI ACGT 2 cut(s) 649, 655
TaqI TCGA 3 cut(s) 15, 578, 1473
TatI WGTACW 1 cut(s) 702
TfiI GAWTC 5 cut(s) 842, 962, 1043, 1133, 1731
Tru1I TTAA 6 cut(s) 423, 681, 1004, 1142, 1148, 1464
Tru9I TTAA 6 cut(s) 423, 681, 1004, 1142, 1148, 1464
TscAI CASTG 2 cut(s) 295, 814
TseFI GTSAC 2 cut(s) 416, 805
TseI GCWGC 5 cut(s) 250, 253, 794, 1715, 1751
Tsp45I GTSAC 2 cut(s) 416, 805
TspDTI ATGAA 9 cut(s) 17, 444, 453, 767, 1113, 1140, 1389, 1726, 1772
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 2 cut(s) 295, 814
Van91I CCANNNNNTGG 1 cut(s) 1117
VpaK11BI GGWCC 4 cut(s) 230, 374, 447, 1400
XapI RAATTY 2 cut(s) 10, 1238
XbaI TCTAGA 1 cut(s) 266
XceI RCATGY 4 cut(s) 880, 914, 924, 1564
XmiI GTMKAC 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 819
XspI CTAG 5 cut(s) 63, 186, 267, 902, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.