Rh2CG016500

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004372
Physical Location & Seq
Forward (+)
1299542 .. 1303247
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG016500.1

Sequence Viewer

Length: 1491 bp
ATGCCACCACATCTATTCACAGGTGCAAAGGTTGAGGGAGAGCAAGGAGCAGCGATTCATGTTGTGTTGCTAGACTTAAGTAAAGGCTCTGTTGTACAAACAGGACCAGAAGCATCTGCAAAGCTCAATATTGTTGTACTGGAAGGTGACTTCAATGAAGAAGTTGAGGATGGTTGGACCAAAGAACATTTTGAAAACCATGAGGTAAAGGAGCGTGAGGGGAAAAGACCACTTCTGACAGGGGATGTACAATTTATTCTTAAGGAAGGGGTGGGTACACTTGGAGAGCTTACTTTTACAGACAATTCTAGCTGGATAAGGAGTAGGAAATTTAGGCTTGGAGTTAAGGTTGCCCCTGGAAACTGTGAAGGAATTCGTATTTGTGAAGCTAAAACAGATGCTTTTTTGGTTAAAGATCACAGGGGAGAATTATACAAGAAACATTACCCACCGGCTTTGCATGATGAGGTGTGGAGATTAGATAGAATAGCAAAGGATGGAGCTCTACACAAGAAGCTGATTAAGTCTGAGATTGTAACTGTTGAAGACTTTCTCCGTCTTCTTGTTAAGGACCCGCAGAAATTAAGAAGTATCCTTGGGAGCGGTATGTCCAACAGGATGTGGGAAAACACCGTGGAGCATGCAAAAACTTGTGTCTTGAGTGGAAAACTCTATGTTTACTACACTGATGAAACTCAAACCAAGGGTGTTGTATTCAACCATATTTATGAACTCAGGGGCCTCATTGCTGATGGGCAATTTTTCTCCCTAGAATCCCTTACTCACAGTCAAAAGGTTTCAGTGGATACTCTGATGAAGAGAGCATATGATAATTGGCATCAAGTCATAGAATACAATGGCATGATGGCTATGACTGCACCTGTGGCAGTCGCTTCCCACAACAATGCCTTAGATCACCATTCTGCAGCTATTCAAGGCAGGCCGCAATATAACTCTGATTTCGGTCAACAGTTTCGAATAGAAAATAACCACCCACCAGTCGTCCCTCAGTTTGTTCCATTTGTGAGGTCTGATCAAACATCTTTAGTTAAATTAAACAACCCACAAACAGCTTTACCTAGCACCACGGGTTACATGTCACTTGGAGGTACATCAAATTTTTCAACAGATTGGTCACGGCCAAATTATGGGAACGGGTTTGAGGACTTTAATGTTGCTGATGAAATCCGGATCAGGAGTTCAGAAATGTTGGAGAGTGATGACATGCAGAGACTGTTAATGAAGACATTTAGTATGGGAGTTGGAATCGGAACAGGACCTAGTTTTGGTAATTCTGATGAAGCTTGTTATAATTATAGTGTACAGTATGAGCCTCATGTAGGTCAATCAGTGGGGAAAGAGCATGGAAAAAGTTCAGGAAAAGCTGTTGTTGGATGGCTTAAGCTCAAAGCTGCTTTAAGGTGGGGAATATTTGTTAGGAAGAGAGCTGCAGAACGGAGAGCTCAGCTTACACTTACAGAACTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

496

Amino Acids

55.54

Weight (kDa)

6.54

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 2 - 140 5.8e-55 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 152 - 218 5.6e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 223 - 285 6.7e-22 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AccB7I CCANNNNNTGG 1 cut(s) 1148
AccBSI CCGCTC 1 cut(s) 603
AccIII TCCGGA 1 cut(s) 1188
AciI CCGC 3 cut(s) 575, 603, 944
AclWI GGATC 1 cut(s) 1199
AcoI YGGCCR 1 cut(s) 1139
AcsI RAATTY 3 cut(s) 329, 372, 1117
AfaI GTAC 6 cut(s) 96, 138, 249, 277, 1111, 1323
AfiI CCNNNNNNNGG 3 cut(s) 1148, 1286, 1340
AflII CTTAAG 3 cut(s) 76, 260, 1400
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 6 cut(s) 154, 194, 545, 718, 935, 1125
AjnI CCWGG 1 cut(s) 355
AloI GAACNNNNNNTCC 2 cut(s) 1183, 1215
Alw21I GWGCWC 2 cut(s) 505, 1465
Alw26I GTCTC 1 cut(s) 1225
AlwI GGATC 1 cut(s) 1199
AlwNI CAGNNNCTG 1 cut(s) 1234
Aor13HI TCCGGA 1 cut(s) 1188
AoxI GGCC 3 cut(s) 739, 941, 1139
ApeKI GCWGC 4 cut(s) 50, 926, 1412, 1448
ApoI RAATTY 3 cut(s) 329, 372, 1117
Asp700I GAANNNNTTC 3 cut(s) 372, 549, 1372
AspS9I GGNCC 5 cut(s) 104, 177, 571, 739, 1277
AsuHPI GGTGA 2 cut(s) 158, 908
AsuII TTCGAA 1 cut(s) 976
AvaII GGWCC 4 cut(s) 104, 177, 571, 1277
BanII GRGCYC 2 cut(s) 505, 1465
BbsI GAAGAC 3 cut(s) 551, 552, 1250
Bbv12I GWGCWC 2 cut(s) 505, 1465
BbvI GCAGC 4 cut(s) 62, 938, 1399, 1435
BccI CCATC 5 cut(s) 164, 491, 746, 859, 1389
BceAI ACGGC 1 cut(s) 1154
BciT130I CCWGG 1 cut(s) 357
BciVI GTATCC 2 cut(s) 602, 799
BclI TGATCA 1 cut(s) 1033
BcoDI GTCTC 1 cut(s) 1225
BfaI CTAG 6 cut(s) 71, 309, 770, 1080, 1281, 1484
BfmI CTRYAG 2 cut(s) 924, 1449
BfrI CTTAAG 3 cut(s) 76, 260, 1400
BfuI GTATCC 2 cut(s) 602, 799
BisI GCNGC 5 cut(s) 51, 927, 944, 1413, 1449
BlpI GCTNAGC 1 cut(s) 1464
BlsI GCNGC 5 cut(s) 52, 928, 945, 1414, 1450
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 4 cut(s) 104, 177, 571, 1277
BmgT120I GGNCC 5 cut(s) 104, 177, 571, 739, 1277
BmiI GGNNCC 2 cut(s) 573, 740
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 3 cut(s) 122, 388, 847
BpiI GAAGAC 3 cut(s) 551, 552, 1250
Bpu1102I GCTNAGC 1 cut(s) 1464
Bpu14I TTCGAA 1 cut(s) 976
BpuEI CTTGAG 1 cut(s) 679
BsaJI CCNNGG 5 cut(s) 355, 595, 633, 702, 1086
BsaWI WCCGGW 1 cut(s) 1188
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 1148, 1286, 1340
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 144, 998
Bse3DI GCAATG 1 cut(s) 744
BseAI TCCGGA 1 cut(s) 1188
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 5 cut(s) 355, 595, 633, 702, 1086
BseGI GGATG 5 cut(s) 175, 250, 502, 624, 1400
BseLI CCNNNNNNNGG 3 cut(s) 1148, 1286, 1340
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 4 cut(s) 519, 748, 1022, 1478
BseNI ACTGG 2 cut(s) 144, 998
BseXI GCAGC 4 cut(s) 62, 938, 1399, 1435
BsgI GTGCAG 1 cut(s) 861
BshFI GGCC 3 cut(s) 741, 943, 1141
BsiHKAI GWGCWC 2 cut(s) 505, 1465
BsiSI CCGG 2 cut(s) 452, 1189
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 3 cut(s) 1148, 1286, 1340
BsmAI GTCTC 1 cut(s) 1225
BsmFI GGGAC 1 cut(s) 989
BsnI GGCC 3 cut(s) 741, 943, 1141
Bsp119I TTCGAA 1 cut(s) 976
Bsp1286I GDGCHC 2 cut(s) 505, 1465
Bsp13I TCCGGA 1 cut(s) 1188
Bsp1407I TGTACA 3 cut(s) 94, 247, 1321
Bsp143I GATC 4 cut(s) 415, 913, 1033, 1191
Bsp1720I GCTNAGC 1 cut(s) 1464
BspACI CCGC 3 cut(s) 575, 603, 944
BspANI GGCC 3 cut(s) 741, 943, 1141
BspCNI CTCAG 4 cut(s) 520, 747, 1021, 1477
BspEI TCCGGA 1 cut(s) 1188
BspLI GGNNCC 2 cut(s) 573, 740
BspMAI CTGCAG 2 cut(s) 928, 1453
BspPI GGATC 1 cut(s) 1199
BspT104I TTCGAA 1 cut(s) 976
BspTI CTTAAG 3 cut(s) 76, 260, 1400
BsrBI CCGCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 451
BsrGI TGTACA 3 cut(s) 94, 247, 1321
BsrI ACTGG 2 cut(s) 144, 998
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 5 cut(s) 355, 595, 633, 702, 1086
BssMI GATC 4 cut(s) 415, 913, 1033, 1191
BssT1I CCWWGG 2 cut(s) 595, 702
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 7 cut(s) 365, 541, 634, 788, 972, 1236, 1326
Bst6I CTCTTC 2 cut(s) 812, 1436
BstAFI CTTAAG 3 cut(s) 76, 260, 1400
BstAUI TGTACA 3 cut(s) 94, 247, 1321
BstBI TTCGAA 1 cut(s) 976
BstC8I GCNNGC 2 cut(s) 642, 941
BstDEI CTNAG 5 cut(s) 528, 734, 910, 1008, 1464
BstDSI CCRYGG 2 cut(s) 633, 1086
BstENI CCTNNNNNAGG 1 cut(s) 1338
BstF5I GGATG 5 cut(s) 175, 250, 502, 624, 1400
BstKTI GATC 4 cut(s) 418, 916, 1036, 1194
BstMAI GTCTC 1 cut(s) 1225
BstMBI GATC 4 cut(s) 415, 913, 1033, 1191
BstMWI GCNNNNNNNGC 2 cut(s) 875, 884
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 3 cut(s) 644, 1099, 1228
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 2 cut(s) 924, 1449
BstV1I GCAGC 4 cut(s) 62, 938, 1399, 1435
BstV2I GAAGAC 3 cut(s) 551, 552, 1250
BsuI GTATCC 2 cut(s) 602, 799
BsuRI GGCC 3 cut(s) 741, 943, 1141
BtgI CCRYGG 2 cut(s) 633, 1086
BtsCI GGATG 5 cut(s) 175, 250, 502, 624, 1400
BtsIMutI CAGTG 3 cut(s) 684, 807, 1356
Cac8I GCNNGC 2 cut(s) 642, 941
CaiI CAGNNNCTG 1 cut(s) 1234
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 5 cut(s) 104, 177, 571, 739, 1277
Csp6I GTAC 6 cut(s) 95, 137, 248, 276, 1110, 1322
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 9 cut(s) 59, 200, 461, 641, 862, 1096, 1225, 1337, 1364
CviQI GTAC 6 cut(s) 95, 137, 248, 276, 1110, 1322
DdeI CTNAG 5 cut(s) 528, 734, 910, 1008, 1464
DpnI GATC 4 cut(s) 417, 915, 1035, 1193
DpnII GATC 4 cut(s) 415, 913, 1033, 1191
EaeI YGGCCR 1 cut(s) 1139
Eam1104I CTCTTC 2 cut(s) 812, 1436
EarI CTCTTC 2 cut(s) 812, 1436
Ecl136II GAGCTC 2 cut(s) 503, 1463
Eco130I CCWWGG 2 cut(s) 595, 702
Eco24I GRGCYC 2 cut(s) 505, 1465
Eco47I GGWCC 4 cut(s) 104, 177, 571, 1277
Eco53kI GAGCTC 2 cut(s) 503, 1463
EcoICRI GAGCTC 2 cut(s) 503, 1463
EcoNI CCTNNNNNAGG 1 cut(s) 1338
EcoO109I RGGNCCY 3 cut(s) 571, 739, 1277
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 595, 702
EcoT38I GRGCYC 2 cut(s) 505, 1465
ErhI CCWWGG 2 cut(s) 595, 702
FaeI CATG 9 cut(s) 62, 203, 464, 644, 865, 1099, 1228, 1340, 1367
FaqI GGGAC 1 cut(s) 989
FatI CATG 9 cut(s) 58, 199, 460, 640, 861, 1095, 1224, 1336, 1363
FauI CCCGC 1 cut(s) 582
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 5 cut(s) 51, 927, 944, 1413, 1449
FokI GGATG 5 cut(s) 182, 257, 509, 631, 1407
FriOI GRGCYC 2 cut(s) 505, 1465
Fsp4HI GCNGC 5 cut(s) 51, 927, 944, 1413, 1449
FspBI CTAG 6 cut(s) 71, 309, 770, 1080, 1281, 1484
GluI GCNGC 5 cut(s) 51, 927, 944, 1413, 1449
HaeIII GGCC 3 cut(s) 741, 943, 1141
HapII CCGG 2 cut(s) 452, 1189
Hin1II CATG 9 cut(s) 62, 203, 464, 644, 865, 1099, 1228, 1340, 1367
HincII GTYRAC 1 cut(s) 968
HindII GTYRAC 1 cut(s) 968
HindIII AAGCTT 1 cut(s) 1302
HinfI GANTC 3 cut(s) 55, 773, 1266
HpaII CCGG 2 cut(s) 452, 1189
HphI GGTGA 2 cut(s) 158, 908
Hpy166II GTNNAC 4 cut(s) 278, 679, 968, 1322
Hpy188I TCNGA 8 cut(s) 237, 529, 813, 958, 1033, 1204, 1271, 1297
Hpy188III TCNNGA 4 cut(s) 658, 1189, 1195, 1377
Hpy8I GTNNAC 4 cut(s) 278, 679, 968, 1322
HpyAV CCTTC 3 cut(s) 137, 260, 362
HpyCH4III ACNGT 7 cut(s) 365, 541, 634, 788, 972, 1236, 1326
HpyCH4V TGCA 8 cut(s) 26, 119, 460, 644, 878, 926, 1228, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 875, 884
HpyF3I CTNAG 5 cut(s) 528, 734, 910, 1008, 1464
Hsp92II CATG 9 cut(s) 62, 203, 464, 644, 865, 1099, 1228, 1340, 1367
Kpn2I TCCGGA 1 cut(s) 1188
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 4 cut(s) 415, 913, 1033, 1191
LmnI GCTCC 5 cut(s) 47, 211, 500, 600, 637
Lsp1109I GCAGC 4 cut(s) 62, 938, 1399, 1435
LweI GCATC 3 cut(s) 122, 388, 847
MaeI CTAG 6 cut(s) 71, 309, 770, 1080, 1281, 1484
MaeIII GTNAC 5 cut(s) 146, 535, 1091, 1098, 1134
MalI GATC 4 cut(s) 417, 915, 1035, 1193
MbiI CCGCTC 1 cut(s) 603
MboI GATC 4 cut(s) 415, 913, 1033, 1191
MboII GAAGA 6 cut(s) 170, 551, 557, 829, 1255, 1453
MhlI GDGCHC 2 cut(s) 505, 1465
MmeI TCCRAC 5 cut(s) 155, 636, 1191, 1243, 1372
MroI TCCGGA 1 cut(s) 1188
MroXI GAANNNNTTC 3 cut(s) 372, 549, 1372
MslI CAYNNNNRTG 2 cut(s) 726, 903
MspCI CTTAAG 3 cut(s) 76, 260, 1400
MspI CCGG 2 cut(s) 452, 1189
MspR9I CCNGG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 875, 884
NdeI CATATG 1 cut(s) 826
NdeII GATC 4 cut(s) 415, 913, 1033, 1191
NlaIII CATG 9 cut(s) 62, 203, 464, 644, 865, 1099, 1228, 1340, 1367
NlaIV GGNNCC 2 cut(s) 573, 740
NmuCI GTSAC 3 cut(s) 146, 1098, 1134
NspI RCATGY 3 cut(s) 644, 1099, 1228
NspV TTCGAA 1 cut(s) 976
PaeI GCATGC 1 cut(s) 644
PciI ACATGT 1 cut(s) 1095
PdmI GAANNNNTTC 3 cut(s) 372, 549, 1372
PfeI GAWTC 3 cut(s) 55, 773, 1266
PflMI CCANNNNNTGG 1 cut(s) 1148
PkrI GCNGC 5 cut(s) 52, 928, 945, 1414, 1450
PpuMI RGGWCCY 2 cut(s) 571, 1277
PscI ACATGT 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 1311
Psp124BI GAGCTC 2 cut(s) 505, 1465
Psp5II RGGWCCY 2 cut(s) 571, 1277
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 573, 740
PspPI GGNCC 5 cut(s) 104, 177, 571, 739, 1277
PspPPI RGGWCCY 2 cut(s) 571, 1277
PstI CTGCAG 2 cut(s) 928, 1453
PstNI CAGNNNCTG 1 cut(s) 1234
RsaI GTAC 6 cut(s) 96, 138, 249, 277, 1111, 1323
RsaNI GTAC 6 cut(s) 95, 137, 248, 276, 1110, 1322
RseI CAYNNNNRTG 2 cut(s) 726, 903
SacI GAGCTC 2 cut(s) 505, 1465
SatI GCNGC 5 cut(s) 51, 927, 944, 1413, 1449
Sau3AI GATC 4 cut(s) 415, 913, 1033, 1191
Sau96I GGNCC 5 cut(s) 104, 177, 571, 739, 1277
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 505, 1465
SfaNI GCATC 3 cut(s) 122, 388, 847
SfcI CTRYAG 2 cut(s) 924, 1449
SfuI TTCGAA 1 cut(s) 976
SinI GGWCC 4 cut(s) 104, 177, 571, 1277
SmiMI CAYNNNNRTG 2 cut(s) 726, 903
SmlI CTYRAG 4 cut(s) 76, 260, 658, 1400
SmoI CTYRAG 4 cut(s) 76, 260, 658, 1400
SphI GCATGC 1 cut(s) 644
SsiI CCGC 3 cut(s) 575, 603, 944
SspI AATATT 2 cut(s) 130, 1431
SspMI CTAG 6 cut(s) 71, 309, 770, 1080, 1281, 1484
SstI GAGCTC 2 cut(s) 505, 1465
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 2 cut(s) 595, 702
TaaI ACNGT 7 cut(s) 365, 541, 634, 788, 972, 1236, 1326
TaqI TCGA 1 cut(s) 976
TaqII GACCGA 1 cut(s) 953
TatI WGTACW 4 cut(s) 94, 136, 247, 1321
TauI GCSGC 1 cut(s) 946
TfiI GAWTC 3 cut(s) 55, 773, 1266
TscAI CASTG 3 cut(s) 691, 807, 1356
TseFI GTSAC 3 cut(s) 146, 1098, 1134
TseI GCWGC 4 cut(s) 50, 926, 1412, 1448
Tsp45I GTSAC 3 cut(s) 146, 1098, 1134
TspDTI ATGAA 8 cut(s) 47, 171, 705, 744, 830, 1197, 1256, 1314
TspGWI ACGGA 2 cut(s) 545, 1471
TspRI CASTG 3 cut(s) 691, 807, 1356
Van91I CCANNNNNTGG 1 cut(s) 1148
Vha464I CTTAAG 3 cut(s) 76, 260, 1400
VpaK11BI GGWCC 4 cut(s) 104, 177, 571, 1277
XagI CCTNNNNNAGG 1 cut(s) 1338
XapI RAATTY 3 cut(s) 329, 372, 1117
XceI RCATGY 3 cut(s) 644, 1099, 1228
XmnI GAANNNNTTC 3 cut(s) 372, 549, 1372
XspI CTAG 6 cut(s) 71, 309, 770, 1080, 1281, 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.