Rw2G001350

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004372
Physical Location & Seq
Forward (+)
1129734 .. 1133647
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001350.1

Sequence Viewer

Length: 1815 bp
ATGGAAAGTTCGAGAAACGTGAGGGCGGAGAAGAGAGGATATGAACAAGGTGTGGATGTGGATGGGGGTTATGATGAGGCAGAACATCGCAGTCATGAATCGAAGAGACCGAAACTGCCCGGTTTGGCAAGTGTGATTGTGGAATCCTTGAAGGTGGATAGCTTGCAAAGACTTGCCTCAACTTTGGAGCCTATGCTCCGCAGAATTGTTAGTGAGGAATTGGAGCGTGCTTTGACGAAAATAGAACGTGTTGAACTGCCTGGAAGGTCTCCACCGCCCAGAATATTAGGCTCAGAAGAGAAAAATCTTCAACTACAATTTAAAACAAGAATGCCACCACATCTATTCACAGGTGCAAAGGTTGAGGGAGAGCAAGGAGCAGCGATTCATGTTGTGTTGCTAGACTTAAGTAAAGGCTCTGTTGTACAAACAGGACCAGAAGCATCTGCAAAGCTCAATATTGTTGTACTGGAAGGTGACTTCAATGAAGAAGTTGAGGATGGTTGGACCAAAGAACATTTTGAAAACCATGAGGTAAAGGAGCGTGAGGGGAAAAGACCACTTCTGACAGGGGATGTACAATTTATTCTTAAGGAAGGGGTGGGTACACTTGGAGAGCTTACTTTTACAGACAATTCTAGCTGGATAAGGAGTAGGAAATTTAGGCTTGGAGTTAAGGTTGCCCCTGGAAACTGTGAAGGAATTCGTATTTGTGAAGCTAAAACAGATGCTTTTTTGGTTAAAGATCACAGGGGAGAATTATACAAGAAACATTACCCACCGGCTTTGCATGATGAGGTGTGGAGATTAGATAGAATAGCAAAGGATGGAGCTCTACACAAGAAGCTGATTAAGTCTGAGATTGTAACTGTTGAAGACTTTCTCCGTCTTCTTGTTAAGGACCCGCAGAAATTAAGAAGTATCCTTGGGAGCGGTATGTCCAACAGGATGTGGGAAAACACCGTGGAGCATGCAAAAACTTGTGTCTTGAGTGGAAAGCTCTATGTTTACTACACTGATGAAACTCAAACCAAGGGTGTTGTATTCAACCATATTTATGAACTCAGGGGCCTCATTGCTGATGGGCAATTTTTCTCCCTAGAATCCCTTACTCACAGTCAAAAGGTTTCAGTGGATACTCTGATGAAGAGAGCATATGATAATTGGCATCAAGTCATAGAATACAATGGCATGATGACTGCACCTGTGGCAGTCGCTTCCCACAACAATGCCTTAGATCACCATTCTGCAGCTATTCAAGGCAGGCCACAATATAACTCTGATTTCGGTCAACAGTTTCGAATAGAAAATAACCACCCACCAGTCGTCCCTCAGTTTGTTCCATTTGTGAGGTCTGATCAAACATCTTTGGTTAAATTAAACAACCCACAAACAGCTTTACCTAGCACAACGGGTTACATGTCACTTGGAGGTACATCAAATTTTTCAACAGATTGGTCACGGCCAAATTATGGGAACGGGTTTGAAGACTTTAATGTTGCTGATGAAATCCGGATCAGGAGTTCAGAAATGTTGGAGAGTGATGACATGCAGAGACTGTTAATGAAGACATTTAGTATGGGAGTTGGAATCGGAACAGGACCTAGTTTTGGTAATTCTGATGAAGCTTGTTATAATTATAGTGTACAGTATGAGCCTCATGTAGGTCAATCAGTGGGGAAAGAGCATGGAAAAAGTTCAGGAAAAGCTGTTGTTGGATGGCTTAAGCTCAAAGCTGCTTTAAGGTGGGGAATATTTGTTAGGAAGAGAGCTGCAGAACGGAGAGCTCAGCTTACACTTACAGAACTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

604

Amino Acids

67.83

Weight (kDa)

6.72

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 103 - 250 2.7e-58 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 262 - 328 7.4e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 333 - 395 8.8e-22 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1635
AccB7I CCANNNNNTGG 1 cut(s) 1472
AccBSI CCGCTC 1 cut(s) 933
AccIII TCCGGA 1 cut(s) 1512
AciI CCGC 5 cut(s) 26, 199, 275, 905, 933
AclWI GGATC 1 cut(s) 1523
AcoI YGGCCR 1 cut(s) 1463
AcsI RAATTY 3 cut(s) 659, 702, 1441
AfaI GTAC 6 cut(s) 426, 468, 579, 607, 1435, 1647
AfiI CCNNNNNNNGG 3 cut(s) 1472, 1610, 1664
AflII CTTAAG 3 cut(s) 406, 590, 1724
AflIII ACRYGT 2 cut(s) 247, 1419
AjnI CCWGG 2 cut(s) 259, 685
AloI GAACNNNNNNTCC 2 cut(s) 1507, 1539
Alw21I GWGCWC 2 cut(s) 835, 1789
Alw26I GTCTC 3 cut(s) 100, 273, 1549
AlwI GGATC 1 cut(s) 1523
AlwNI CAGNNNCTG 1 cut(s) 1558
Aor13HI TCCGGA 1 cut(s) 1512
AoxI GGCC 3 cut(s) 1069, 1265, 1463
ApeKI GCWGC 4 cut(s) 380, 1250, 1736, 1772
ApoI RAATTY 3 cut(s) 659, 702, 1441
Asp700I GAANNNNTTC 3 cut(s) 702, 879, 1696
AspS9I GGNCC 5 cut(s) 434, 507, 901, 1069, 1601
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 488, 1232
AsuII TTCGAA 1 cut(s) 1300
AvaII GGWCC 4 cut(s) 434, 507, 901, 1601
BanII GRGCYC 2 cut(s) 835, 1789
BbsI GAAGAC 4 cut(s) 881, 882, 1494, 1574
Bbv12I GWGCWC 2 cut(s) 835, 1789
BbvI GCAGC 4 cut(s) 392, 1262, 1723, 1759
BccI CCATC 5 cut(s) 56, 494, 821, 1076, 1713
BceAI ACGGC 1 cut(s) 1478
BciT130I CCWGG 2 cut(s) 261, 687
BciVI GTATCC 2 cut(s) 932, 1129
BclI TGATCA 1 cut(s) 1357
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 3 cut(s) 100, 273, 1549
BfaI CTAG 6 cut(s) 401, 639, 1100, 1404, 1605, 1808
BfmI CTRYAG 2 cut(s) 1248, 1773
BfrI CTTAAG 3 cut(s) 406, 590, 1724
BfuI GTATCC 2 cut(s) 932, 1129
BisI GCNGC 4 cut(s) 381, 1251, 1737, 1773
BlpI GCTNAGC 1 cut(s) 1788
BlsI GCNGC 4 cut(s) 382, 1252, 1738, 1774
Bme1390I CCNGG 3 cut(s) 120, 261, 687
Bme18I GGWCC 4 cut(s) 434, 507, 901, 1601
BmgT120I GGNCC 5 cut(s) 434, 507, 901, 1069, 1601
BmiI GGNNCC 3 cut(s) 189, 903, 1070
BmrFI CCNGG 3 cut(s) 120, 261, 687
BmsI GCATC 3 cut(s) 452, 718, 1177
BpiI GAAGAC 4 cut(s) 881, 882, 1494, 1574
Bpu1102I GCTNAGC 1 cut(s) 1788
Bpu14I TTCGAA 1 cut(s) 1300
BpuEI CTTGAG 1 cut(s) 1009
BpuMI CCSGG 1 cut(s) 120
BsaI GGTCTC 2 cut(s) 100, 273
BsaJI CCNNGG 4 cut(s) 685, 925, 963, 1032
BsaWI WCCGGW 1 cut(s) 1512
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 3 cut(s) 1472, 1610, 1664
Bse118I RCCGGY 1 cut(s) 781
Bse1I ACTGG 2 cut(s) 474, 1322
Bse3DI GCAATG 1 cut(s) 1074
BseAI TCCGGA 1 cut(s) 1512
BseBI CCWGG 2 cut(s) 261, 687
BseDI CCNNGG 4 cut(s) 685, 925, 963, 1032
BseGI GGATG 7 cut(s) 61, 67, 505, 580, 832, 954, 1724
BseLI CCNNNNNNNGG 3 cut(s) 1472, 1610, 1664
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 5 cut(s) 306, 849, 1078, 1346, 1802
BseNI ACTGG 2 cut(s) 474, 1322
BseXI GCAGC 4 cut(s) 392, 1262, 1723, 1759
BsgI GTGCAG 1 cut(s) 1185
BshFI GGCC 3 cut(s) 1071, 1267, 1465
BsiHKAI GWGCWC 2 cut(s) 835, 1789
BsiSI CCGG 3 cut(s) 120, 782, 1513
BslFI GGGAC 1 cut(s) 1313
BslI CCNNNNNNNGG 3 cut(s) 1472, 1610, 1664
BsmAI GTCTC 3 cut(s) 100, 273, 1549
BsmFI GGGAC 1 cut(s) 1313
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 3 cut(s) 1071, 1267, 1465
Bso31I GGTCTC 2 cut(s) 100, 273
Bsp119I TTCGAA 1 cut(s) 1300
Bsp1286I GDGCHC 2 cut(s) 835, 1789
Bsp13I TCCGGA 1 cut(s) 1512
Bsp1407I TGTACA 3 cut(s) 424, 577, 1645
Bsp143I GATC 4 cut(s) 745, 1237, 1357, 1515
Bsp1720I GCTNAGC 1 cut(s) 1788
BspACI CCGC 5 cut(s) 26, 199, 275, 905, 933
BspANI GGCC 3 cut(s) 1071, 1267, 1465
BspCNI CTCAG 5 cut(s) 305, 850, 1077, 1345, 1801
BspEI TCCGGA 1 cut(s) 1512
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 3 cut(s) 189, 903, 1070
BspMAI CTGCAG 2 cut(s) 1252, 1777
BspPI GGATC 1 cut(s) 1523
BspT104I TTCGAA 1 cut(s) 1300
BspTI CTTAAG 3 cut(s) 406, 590, 1724
BspTNI GGTCTC 2 cut(s) 100, 273
BsrBI CCGCTC 1 cut(s) 933
BsrDI GCAATG 1 cut(s) 1074
BsrFI RCCGGY 1 cut(s) 781
BsrGI TGTACA 3 cut(s) 424, 577, 1645
BsrI ACTGG 2 cut(s) 474, 1322
BssAI RCCGGY 1 cut(s) 781
BssECI CCNNGG 4 cut(s) 685, 925, 963, 1032
BssMI GATC 4 cut(s) 745, 1237, 1357, 1515
BssT1I CCWWGG 2 cut(s) 925, 1032
Bst2UI CCWGG 2 cut(s) 261, 687
Bst4CI ACNGT 7 cut(s) 695, 871, 964, 1118, 1296, 1560, 1650
Bst6I CTCTTC 5 cut(s) 26, 98, 291, 1142, 1760
BstAFI CTTAAG 3 cut(s) 406, 590, 1724
BstAUI TGTACA 3 cut(s) 424, 577, 1645
BstBI TTCGAA 1 cut(s) 1300
BstC8I GCNNGC 4 cut(s) 164, 228, 972, 1265
BstDEI CTNAG 6 cut(s) 292, 858, 1064, 1234, 1332, 1788
BstDSI CCRYGG 1 cut(s) 963
BstENI CCTNNNNNAGG 1 cut(s) 1662
BstF5I GGATG 7 cut(s) 61, 67, 505, 580, 832, 954, 1724
BstKTI GATC 4 cut(s) 748, 1240, 1360, 1518
BstMAI GTCTC 3 cut(s) 100, 273, 1549
BstMBI GATC 4 cut(s) 745, 1237, 1357, 1515
BstMWI GCNNNNNNNGC 1 cut(s) 1208
BstNI CCWGG 2 cut(s) 261, 687
BstNSI RCATGY 3 cut(s) 974, 1423, 1552
BstSCI CCNGG 3 cut(s) 118, 259, 685
BstSFI CTRYAG 2 cut(s) 1248, 1773
BstV1I GCAGC 4 cut(s) 392, 1262, 1723, 1759
BstV2I GAAGAC 4 cut(s) 881, 882, 1494, 1574
BsuI GTATCC 2 cut(s) 932, 1129
BsuRI GGCC 3 cut(s) 1071, 1267, 1465
BtgI CCRYGG 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 7 cut(s) 61, 67, 505, 580, 832, 954, 1724
BtsIMutI CAGTG 3 cut(s) 1014, 1137, 1680
Cac8I GCNNGC 4 cut(s) 164, 228, 972, 1265
CaiI CAGNNNCTG 1 cut(s) 1558
CciI TCATGA 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 781
Cfr13I GGNCC 5 cut(s) 434, 507, 901, 1069, 1601
Csp6I GTAC 6 cut(s) 425, 467, 578, 606, 1434, 1646
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviQI GTAC 6 cut(s) 425, 467, 578, 606, 1434, 1646
DdeI CTNAG 6 cut(s) 292, 858, 1064, 1234, 1332, 1788
DpnI GATC 4 cut(s) 747, 1239, 1359, 1517
DpnII GATC 4 cut(s) 745, 1237, 1357, 1515
DraI TTTAAA 1 cut(s) 322
EaeI YGGCCR 1 cut(s) 1463
Eam1104I CTCTTC 5 cut(s) 26, 98, 291, 1142, 1760
EarI CTCTTC 5 cut(s) 26, 98, 291, 1142, 1760
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 2 cut(s) 833, 1787
Eco130I CCWWGG 2 cut(s) 925, 1032
Eco24I GRGCYC 2 cut(s) 835, 1789
Eco31I GGTCTC 2 cut(s) 100, 273
Eco47I GGWCC 4 cut(s) 434, 507, 901, 1601
Eco53kI GAGCTC 2 cut(s) 833, 1787
EcoICRI GAGCTC 2 cut(s) 833, 1787
EcoNI CCTNNNNNAGG 1 cut(s) 1662
EcoO109I RGGNCCY 3 cut(s) 901, 1069, 1601
EcoRI GAATTC 1 cut(s) 702
EcoRII CCWGG 2 cut(s) 259, 685
EcoT14I CCWWGG 2 cut(s) 925, 1032
EcoT38I GRGCYC 2 cut(s) 835, 1789
ErhI CCWWGG 2 cut(s) 925, 1032
FaqI GGGAC 1 cut(s) 1313
FauI CCCGC 1 cut(s) 912
FauNDI CATATG 1 cut(s) 1156
FbaI TGATCA 1 cut(s) 1357
Fnu4HI GCNGC 4 cut(s) 381, 1251, 1737, 1773
FokI GGATG 7 cut(s) 68, 74, 512, 587, 839, 961, 1731
FriOI GRGCYC 2 cut(s) 835, 1789
Fsp4HI GCNGC 4 cut(s) 381, 1251, 1737, 1773
FspBI CTAG 6 cut(s) 401, 639, 1100, 1404, 1605, 1808
GluI GCNGC 4 cut(s) 381, 1251, 1737, 1773
HaeIII GGCC 3 cut(s) 1071, 1267, 1465
HapII CCGG 3 cut(s) 120, 782, 1513
HincII GTYRAC 1 cut(s) 1292
HindII GTYRAC 1 cut(s) 1292
HindIII AAGCTT 1 cut(s) 1626
HinfI GANTC 5 cut(s) 98, 143, 385, 1103, 1590
HpaII CCGG 3 cut(s) 120, 782, 1513
HphI GGTGA 2 cut(s) 488, 1232
Hpy166II GTNNAC 4 cut(s) 608, 1009, 1292, 1646
Hpy188I TCNGA 9 cut(s) 295, 567, 859, 1143, 1282, 1357, 1528, 1595, 1621
Hpy188III TCNNGA 6 cut(s) 12, 95, 988, 1513, 1519, 1701
Hpy8I GTNNAC 4 cut(s) 608, 1009, 1292, 1646
HpyAV CCTTC 5 cut(s) 145, 258, 467, 590, 692
HpyCH4III ACNGT 7 cut(s) 695, 871, 964, 1118, 1296, 1560, 1650
HpyCH4IV ACGT 2 cut(s) 18, 247
HpyCH4V TGCA 9 cut(s) 166, 356, 449, 790, 974, 1202, 1250, 1552, 1775
HpyF10VI GCNNNNNNNGC 1 cut(s) 1208
HpyF3I CTNAG 6 cut(s) 292, 858, 1064, 1234, 1332, 1788
HpySE526I ACGT 2 cut(s) 18, 247
Kpn2I TCCGGA 1 cut(s) 1512
Ksp22I TGATCA 1 cut(s) 1357
Kzo9I GATC 4 cut(s) 745, 1237, 1357, 1515
LmnI GCTCC 8 cut(s) 187, 201, 223, 377, 541, 830, 930, 967
Lsp1109I GCAGC 4 cut(s) 392, 1262, 1723, 1759
LweI GCATC 3 cut(s) 452, 718, 1177
MaeI CTAG 6 cut(s) 401, 639, 1100, 1404, 1605, 1808
MaeII ACGT 2 cut(s) 18, 247
MaeIII GTNAC 5 cut(s) 476, 865, 1415, 1422, 1458
MalI GATC 4 cut(s) 747, 1239, 1359, 1517
MbiI CCGCTC 1 cut(s) 933
MboI GATC 4 cut(s) 745, 1237, 1357, 1515
MhlI GDGCHC 2 cut(s) 835, 1789
MmeI TCCRAC 5 cut(s) 485, 966, 1515, 1567, 1696
MroI TCCGGA 1 cut(s) 1512
MroXI GAANNNNTTC 3 cut(s) 702, 879, 1696
MslI CAYNNNNRTG 2 cut(s) 1056, 1227
MspCI CTTAAG 3 cut(s) 406, 590, 1724
MspI CCGG 3 cut(s) 120, 782, 1513
MspR9I CCNGG 3 cut(s) 120, 261, 687
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 2 cut(s) 261, 687
MwoI GCNNNNNNNGC 1 cut(s) 1208
NciI CCSGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 1156
NdeII GATC 4 cut(s) 745, 1237, 1357, 1515
NlaIV GGNNCC 3 cut(s) 189, 903, 1070
NmuCI GTSAC 3 cut(s) 476, 1422, 1458
NspI RCATGY 3 cut(s) 974, 1423, 1552
NspV TTCGAA 1 cut(s) 1300
PaeI GCATGC 1 cut(s) 974
PagI TCATGA 1 cut(s) 94
PciI ACATGT 1 cut(s) 1419
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 336
PdmI GAANNNNTTC 3 cut(s) 702, 879, 1696
PfeI GAWTC 5 cut(s) 98, 143, 385, 1103, 1590
PflMI CCANNNNNTGG 1 cut(s) 1472
PkrI GCNGC 4 cut(s) 382, 1252, 1738, 1774
PpuMI RGGWCCY 2 cut(s) 901, 1601
PscI ACATGT 1 cut(s) 1419
PsiI TTATAA 1 cut(s) 1635
Psp124BI GAGCTC 2 cut(s) 835, 1789
Psp5II RGGWCCY 2 cut(s) 901, 1601
Psp6I CCWGG 2 cut(s) 259, 685
PspGI CCWGG 2 cut(s) 259, 685
PspN4I GGNNCC 3 cut(s) 189, 903, 1070
PspPI GGNCC 5 cut(s) 434, 507, 901, 1069, 1601
PspPPI RGGWCCY 2 cut(s) 901, 1601
PstI CTGCAG 2 cut(s) 1252, 1777
PstNI CAGNNNCTG 1 cut(s) 1558
RsaI GTAC 6 cut(s) 426, 468, 579, 607, 1435, 1647
RsaNI GTAC 6 cut(s) 425, 467, 578, 606, 1434, 1646
RseI CAYNNNNRTG 2 cut(s) 1056, 1227
SacI GAGCTC 2 cut(s) 835, 1789
SatI GCNGC 4 cut(s) 381, 1251, 1737, 1773
Sau3AI GATC 4 cut(s) 745, 1237, 1357, 1515
Sau96I GGNCC 5 cut(s) 434, 507, 901, 1069, 1601
ScrFI CCNGG 3 cut(s) 120, 261, 687
SduI GDGCHC 2 cut(s) 835, 1789
SfaNI GCATC 3 cut(s) 452, 718, 1177
SfcI CTRYAG 2 cut(s) 1248, 1773
SfuI TTCGAA 1 cut(s) 1300
SinI GGWCC 4 cut(s) 434, 507, 901, 1601
SmiMI CAYNNNNRTG 2 cut(s) 1056, 1227
SmlI CTYRAG 4 cut(s) 406, 590, 988, 1724
SmoI CTYRAG 4 cut(s) 406, 590, 988, 1724
SphI GCATGC 1 cut(s) 974
SsiI CCGC 5 cut(s) 26, 199, 275, 905, 933
SspI AATATT 3 cut(s) 285, 460, 1755
SspMI CTAG 6 cut(s) 401, 639, 1100, 1404, 1605, 1808
SstI GAGCTC 2 cut(s) 835, 1789
StyD4I CCNGG 3 cut(s) 118, 259, 685
StyI CCWWGG 2 cut(s) 925, 1032
TaaI ACNGT 7 cut(s) 695, 871, 964, 1118, 1296, 1560, 1650
TaiI ACGT 2 cut(s) 21, 250
TaqI TCGA 3 cut(s) 11, 101, 1300
TaqII GACCGA 2 cut(s) 124, 1277
TatI WGTACW 4 cut(s) 424, 466, 577, 1645
TfiI GAWTC 5 cut(s) 98, 143, 385, 1103, 1590
TscAI CASTG 3 cut(s) 1021, 1137, 1680
TseFI GTSAC 3 cut(s) 476, 1422, 1458
TseI GCWGC 4 cut(s) 380, 1250, 1736, 1772
Tsp45I GTSAC 3 cut(s) 476, 1422, 1458
TspGWI ACGGA 2 cut(s) 875, 1795
TspRI CASTG 3 cut(s) 1021, 1137, 1680
Van91I CCANNNNNTGG 1 cut(s) 1472
Vha464I CTTAAG 3 cut(s) 406, 590, 1724
VpaK11BI GGWCC 4 cut(s) 434, 507, 901, 1601
XagI CCTNNNNNAGG 1 cut(s) 1662
XapI RAATTY 3 cut(s) 659, 702, 1441
XceI RCATGY 3 cut(s) 974, 1423, 1552
XmnI GAANNNNTTC 3 cut(s) 702, 879, 1696
XspI CTAG 6 cut(s) 401, 639, 1100, 1404, 1605, 1808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.