pycom15g13970

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004372
Physical Location & Seq
Forward (+)
9510008 .. 9511813
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13970.1

Sequence Viewer

Length: 621 bp
ATGGAAAGTTCGGGGAGTACGAGGGTGGAGAAGAGAGGTCATGAATTGGATGCCGATGCAGATGATCATCCTCCCGAGCCGAAGAAGCAGAGATTGCCTGCTTTGGGAAGTGTAATTGTGGAATCTTTGAAGGTAGATAGCTTGCAAAGACTTTGCTCGTCGTTGGAGCCTCTTCTCCGCCGAATTGTTAGTGAAGAAGTCGAGCGCGCTTTGACAAAGTTAGACCATGCTGAACTGGCTGGAAGGTCTCAACCGCCGAGAATACAGGGTCCAGAAGGAAAAACCCTGCAACTACAATTCAAAACAAGAATGCCACCTCATCTATTCACAGGTGCAAAGGTTGAAGGAGAGCAAGGAGCAGCAATTCATGTTGTTTTGGTCGACCTCAGCACAGGCAGTGTTATGCAAACAGGACCCGAATCCGCTGCAAAACTGAATGTTGTAGTGCTGGAAGGTGACTTCAATGGGGAAGCTGAGGATAATTGGACAAAAGAACACTTTGAGAACCACGAAGTAAAGGAACGTGAGGGGAAAAGGCCGCTTCTGACAGGGGATCGTTCAGGTTGTTCTCAAGGAAGGGGTAGGTACTGTAGGAGATCTTACCTTCACCGACAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

207

Amino Acids

22.92

Weight (kDa)

6.46

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 96 - 186 3.4e-30 Calmodulin binding protein-like N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 207
AciI CCGC 4 cut(s) 178, 254, 423, 539
AclWI GGATC 1 cut(s) 561
AfaI GTAC 2 cut(s) 19, 587
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 130, 301, 344, 463
AluBI AGCT 2 cut(s) 141, 473
AluI AGCT 2 cut(s) 141, 473
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 1 cut(s) 561
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 2 cut(s) 359, 425
AspLEI GCGC 2 cut(s) 207, 209
AspS9I GGNCC 2 cut(s) 269, 413
AsuHPI GGTGA 2 cut(s) 467, 599
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 2 cut(s) 269, 413
BbvCI CCTCAGC 2 cut(s) 386, 474
BbvI GCAGC 2 cut(s) 371, 412
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 619
BfmI CTRYAG 1 cut(s) 589
BglII AGATCT 1 cut(s) 596
BisI GCNGC 3 cut(s) 360, 426, 539
BlsI GCNGC 3 cut(s) 361, 427, 540
Bme18I GGWCC 2 cut(s) 269, 413
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 269, 413
BmiI GGNNCC 3 cut(s) 168, 270, 415
BmsI GCATC 2 cut(s) 40, 46
Bpu10I CCTNAGC 2 cut(s) 386, 474
BpuEI CTTGAG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 66
BseGI GGATG 2 cut(s) 55, 67
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 2 cut(s) 400, 465
BseNI ACTGG 1 cut(s) 240
BsePI GCGCGC 1 cut(s) 205
BseXI GCAGC 2 cut(s) 371, 412
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 1 cut(s) 538
Bso31I GGTCTC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 74
Bsp143I GATC 3 cut(s) 64, 553, 596
BspACI CCGC 4 cut(s) 178, 254, 423, 539
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 2 cut(s) 399, 466
BspFNI CGCG 1 cut(s) 207
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 3 cut(s) 168, 270, 415
BspPI GGATC 1 cut(s) 561
BspTNI GGTCTC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 240
BssHII GCGCGC 1 cut(s) 205
BssMI GATC 3 cut(s) 64, 553, 596
Bst4CI ACNGT 1 cut(s) 590
Bst6I CTCTTC 2 cut(s) 26, 177
BstAPI GCANNNNNTGC 1 cut(s) 94
BstC8I GCNNGC 3 cut(s) 99, 143, 207
BstDEI CTNAG 2 cut(s) 386, 474
BstF5I GGATG 2 cut(s) 55, 67
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 2 cut(s) 207, 209
BstKTI GATC 3 cut(s) 67, 556, 599
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 3 cut(s) 64, 553, 596
BstMWI GCNNNNNNNGC 3 cut(s) 85, 94, 236
BstSFI CTRYAG 1 cut(s) 589
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 371, 412
BstX2I RGATCY 1 cut(s) 596
BstYI RGATCY 1 cut(s) 596
BsuRI GGCC 1 cut(s) 538
BtsCI GGATG 2 cut(s) 55, 67
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 3 cut(s) 99, 143, 207
CciI TCATGA 1 cut(s) 40
CfoI GCGC 2 cut(s) 207, 209
Cfr13I GGNCC 2 cut(s) 269, 413
Csp6I GTAC 2 cut(s) 18, 586
CviAII CATG 3 cut(s) 41, 227, 368
CviJI RGCY 6 cut(s) 79, 141, 169, 239, 473, 538
CviKI_1 RGCY 6 cut(s) 79, 141, 169, 239, 473, 538
CviQI GTAC 2 cut(s) 18, 586
DdeI CTNAG 2 cut(s) 386, 474
DpnI GATC 3 cut(s) 66, 555, 598
DpnII GATC 3 cut(s) 64, 553, 596
Eam1104I CTCTTC 2 cut(s) 26, 177
EarI CTCTTC 2 cut(s) 26, 177
EciI GGCGGA 1 cut(s) 167
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 269, 413
Eco88I CYCGRG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 413
FaeI CATG 3 cut(s) 44, 230, 371
FaiI YATR 4 cut(s) 42, 228, 369, 404
FatI CATG 3 cut(s) 40, 226, 367
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 360, 426, 539
FokI GGATG 2 cut(s) 54, 62
Fsp4HI GCNGC 3 cut(s) 360, 426, 539
FspBI CTAG 1 cut(s) 619
GlaI GCGC 2 cut(s) 206, 208
GluI GCNGC 3 cut(s) 360, 426, 539
HaeIII GGCC 1 cut(s) 538
HhaI GCGC 2 cut(s) 207, 209
Hin1II CATG 3 cut(s) 44, 230, 371
Hin6I GCGC 2 cut(s) 205, 207
HinP1I GCGC 2 cut(s) 205, 207
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 2 cut(s) 122, 419
HphI GGTGA 2 cut(s) 467, 599
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 1 cut(s) 546
Hpy188III TCNNGA 3 cut(s) 41, 74, 272
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 7 cut(s) 124, 237, 269, 338, 446, 570, 614
HpyCH4III ACNGT 1 cut(s) 590
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 6 cut(s) 59, 145, 289, 335, 406, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 94, 236
HpyF3I CTNAG 2 cut(s) 386, 474
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 3 cut(s) 44, 230, 371
HspAI GCGC 2 cut(s) 205, 207
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 3 cut(s) 64, 553, 596
LmnI GCTCC 2 cut(s) 166, 356
Lsp1109I GCAGC 2 cut(s) 371, 412
LweI GCATC 2 cut(s) 40, 46
MaeI CTAG 1 cut(s) 619
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 1 cut(s) 455
MalI GATC 3 cut(s) 66, 555, 598
MboI GATC 3 cut(s) 64, 553, 596
MboII GAAGA 4 cut(s) 43, 94, 164, 206
MflI RGATCY 1 cut(s) 596
MluCI AATT 7 cut(s) 44, 114, 183, 296, 363, 481, 614
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 8 cut(s) 15, 29, 81, 180, 327, 395, 469, 520
MspA1I CMGCKG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 315
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 3 cut(s) 85, 94, 236
NdeII GATC 3 cut(s) 64, 553, 596
NlaIII CATG 3 cut(s) 44, 230, 371
NlaIV GGNNCC 3 cut(s) 168, 270, 415
NmeAIII GCCGAG 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 455
PagI TCATGA 1 cut(s) 40
PauI GCGCGC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 122, 419
PkrI GCNGC 3 cut(s) 361, 427, 540
PpuMI RGGWCCY 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 168, 270, 415
PspPI GGNCC 2 cut(s) 269, 413
PspPPI RGGWCCY 1 cut(s) 413
PsuI RGATCY 1 cut(s) 596
PteI GCGCGC 1 cut(s) 205
RsaI GTAC 2 cut(s) 19, 587
RsaNI GTAC 2 cut(s) 18, 586
SalI GTCGAC 1 cut(s) 380
SatI GCNGC 3 cut(s) 360, 426, 539
Sau3AI GATC 3 cut(s) 64, 553, 596
Sau96I GGNCC 2 cut(s) 269, 413
SfaNI GCATC 2 cut(s) 40, 46
SfcI CTRYAG 1 cut(s) 589
SinI GGWCC 2 cut(s) 269, 413
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
Sse9I AATT 7 cut(s) 44, 114, 183, 296, 363, 481, 614
SsiI CCGC 4 cut(s) 178, 254, 423, 539
SspMI CTAG 1 cut(s) 619
TaaI ACNGT 1 cut(s) 590
TaiI ACGT 1 cut(s) 526
TaqI TCGA 2 cut(s) 201, 381
TasI AATT 7 cut(s) 44, 114, 183, 296, 363, 481, 614
TauI GCSGC 1 cut(s) 541
TfiI GAWTC 2 cut(s) 122, 419
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 2 cut(s) 359, 425
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 57, 356
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 269, 413
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.