AT4G31000

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004372
Physical Location & Seq
Forward (+)
15103120 .. 15105979
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31000.2

Sequence Viewer

Length: 1689 bp
ATGGAAAATTCTATGAACAACAGAGGACATGGACACAATCAAGAACATGCAGACAACTTGCCAGAATCAAAGAGACAGAAGCTTCCTGCTTTGGCAAGTGTCATAGTGGAAGCTGTCAAGGTAGATAGTCTGCAGAGGCTATGCTCGTCTTTGGAACCATTATTTCGAAGAATTGTCAGTGAAGAGGTGGAACGTGCCATATCAAGGTTGGAGAACTCGAAATCTACTTCAAGATCTACAGAACCGAATAAGATTCAAGGCCTCGATGGAAGAAATCTGCAACTTCGGTTCAGAACACGAATGCCTCCTCATTTATTCACAGGTGGGAAAGTCGAGGGAGAGCAAGGCTCAGCCATTCACGTGGTTCTTATAGATGCAAACACCGGAAATGTAATCCAAACAGGAGAAGAATCAATGACAAAGCTGAATATTGTCGTGTTAGATGGAGACTTCAATGATGAAGACGACAAAGATTGGACGAGAGAACATTTTGAGAGCTTTGAAGTGAAGGAACGCGAAGGAAAACGTCCCATCTTGACTGGTGACAGACATGTGATAATTAAAGAAGGTGTAGGAACTCTTGGAAAACTTACTTTCACTGACAATTCGAGCTGGATTAGGAGCAGAAAGTTTAGACTTGGTGTTAAGCCTGCAACGGGATTTCACATCCGTGAAGCTAAAACAGAACCATTTGCTGTTAAAGATCACAGAGGAGAACTATACAAGAAACATTATCCACCGGTTTTACATGATGAAGTCTGGAGATTGGATAAAATAGCGAAAGATGGAGCGCTACACAAGAAGCTACTAAAATCTAACATTGTGACTGTTGAAGACTTCCTACAGATACTCATGAAGGATCCGCAGAAACTAAGAAGCTTACTTGGGAGTGGAATGTCAAATAGAATGTGGGATAACACGGTAGAGCACGCAAAGACTTGTGTCTTAGGTGGGAAACTTTACGCCTATTACACAGATCAAACACACCAGACAGCTGTTGTCTTTAATCATATATATGAATTTCAAGGCCTAATTGCCAATGGCCACTTCTTGTCTTCGGAATCTCTTAACCATGACCAAAAGATTTCTGCAGACACTTTGGTGAAGACTGCTTACGAGAACTGGCATAAAGTGGTTGAGTATGGCGGTAAGCTCTTGAATTGCTTACCAGTTGCTAAGAAGGGAATAAAAAGCTTGCCAGAGCCAAAAATGGCTCAGAACCATCAAACGCAGCAGAAGGCTCTGCAATGCCAACAGACTGTGAATGGCTACTCATCAGATCCTCAGCATCTGATAGAGTATCCTTTTGTCCAACAACCTTGCTATCAGTTACGAGACTACACATCGATGGAGAGTTCTTCAGTTTCAGGCTCATATAATGGAGGCCTTGAAGATATTTTCACGGAGGAAATTCGAGCGAGGAGTTCTGAGATGCTCGAGACGGATAACATGCAGAGATTGCTCAAGACATTTGGGATATCAGGCGGGTTTGGTAACAGGGATGAGTCCATTTACGGTTTCAGTGATCAGTACGAAGCTCAGATAGACAAGGGTTATATGAGAGAAGGAGGTAGAGGCGCAGGGAAAGCTGTAGTGGGATGGCTTAAGCTCAAAGCTGCATTGAGATGGGGTATCTTTATACGCAAGAAAGCTGCAGAGAGGAGGCCTCAAATTGTGGAGATCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

562

Amino Acids

63.68

Weight (kDa)

8.51

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 88 - 212 3.9e-47 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 228 - 293 1.9e-31 Calmodulin binding protein central domain
Calmod_bind_C PF20452 298 - 360 2e-20 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 516
AciI CCGC 3 cut(s) 863, 1146, 1485
AclWI GGATC 3 cut(s) 854, 867, 1274
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 3 cut(s) 7, 1019, 1410
AcuI CTGAAG 1 cut(s) 1344
AcvI CACGTG 1 cut(s) 361
AfaI GTAC 1 cut(s) 1532
AfeI AGCGCT 1 cut(s) 792
AfiI CCNNNNNNNGG 2 cut(s) 204, 656
AflII CTTAAG 1 cut(s) 1604
AflIII ACRYGT 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 739
AgsI TTSAA 8 cut(s) 231, 257, 454, 503, 833, 1025, 1159, 1391
AjuI GAANNNNNNNTTGG 2 cut(s) 1031, 1063
AleI CACNNNNGTG 2 cut(s) 669, 1100
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 4 cut(s) 67, 441, 1329, 1433
AlwI GGATC 3 cut(s) 854, 867, 1274
AlwNI CAGNNNCTG 1 cut(s) 1291
Ama87I CYCGRG 1 cut(s) 1436
Aor51HI AGCGCT 1 cut(s) 792
AoxI GGCC 5 cut(s) 259, 1027, 1042, 1384, 1664
ApeKI GCWGC 3 cut(s) 1231, 1616, 1652
ApoI RAATTY 3 cut(s) 7, 1019, 1410
AsiGI ACCGGT 1 cut(s) 739
AspLEI GCGC 2 cut(s) 793, 1580
AsuHPI GGTGA 2 cut(s) 554, 1114
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 1436
BalI TGGCCA 1 cut(s) 1044
BamHI GGATCC 1 cut(s) 859
BarI GAAGNNNNNNTAC 4 cut(s) 825, 857, 1097, 1129
BbrPI CACGTG 1 cut(s) 361
BbsI GAAGAC 4 cut(s) 468, 840, 1047, 1112
Bbv12I GWGCWC 1 cut(s) 930
BbvCI CCTCAGC 1 cut(s) 1284
BbvI GCAGC 3 cut(s) 1243, 1603, 1639
BccI CCATC 8 cut(s) 260, 437, 539, 779, 1230, 1342, 1593, 1620
BciVI GTATCC 1 cut(s) 1311
BclI TGATCA 1 cut(s) 1525
BcoDI GTCTC 4 cut(s) 67, 441, 1329, 1433
BfmI CTRYAG 6 cut(s) 131, 237, 842, 1089, 1590, 1653
BfoI RGCGCY 1 cut(s) 794
BfrI CTTAAG 1 cut(s) 1604
BfuI GTATCC 1 cut(s) 1311
BglII AGATCT 1 cut(s) 233
BisI GCNGC 3 cut(s) 1232, 1617, 1653
BlpI GCTNAGC 1 cut(s) 349
BlsI GCNGC 3 cut(s) 1233, 1618, 1654
BmeT110I CYCGRG 1 cut(s) 1436
BmiI GGNNCC 2 cut(s) 156, 861
BmsI GCATC 3 cut(s) 364, 1297, 1422
BoxI GACNNNNGTC 1 cut(s) 941
BpiI GAAGAC 4 cut(s) 468, 840, 1047, 1112
BplI GAGNNNNNCTC 4 cut(s) 332, 364, 1651, 1683
BpmI CTGGAG 1 cut(s) 781
Bpu10I CCTNAGC 1 cut(s) 1284
Bpu1102I GCTNAGC 1 cut(s) 349
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 1448
Bsa29I ATCGAT 1 cut(s) 1346
BsaAI YACGTR 1 cut(s) 361
BsaWI WCCGGW 2 cut(s) 383, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 656
Bse118I RCCGGY 1 cut(s) 739
Bse1I ACTGG 3 cut(s) 544, 1127, 1169
Bse3DI GCAATG 1 cut(s) 1253
BseCI ATCGAT 1 cut(s) 1346
BseGI GGATG 3 cut(s) 666, 1507, 1604
BseLI CCNNNNNNNGG 2 cut(s) 204, 656
BseMI GCAATG 1 cut(s) 1253
BseMII CTCAG 5 cut(s) 363, 1229, 1298, 1419, 1553
BseNI ACTGG 3 cut(s) 544, 1127, 1169
BseRI GAGGAG 4 cut(s) 297, 726, 1435, 1675
BseXI GCAGC 3 cut(s) 1243, 1603, 1639
Bsh1236I CGCG 1 cut(s) 516
BshFI GGCC 5 cut(s) 261, 1029, 1044, 1386, 1666
BshTI ACCGGT 1 cut(s) 739
BshVI ATCGAT 1 cut(s) 1346
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 2 cut(s) 384, 740
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 204, 656
BsmAI GTCTC 4 cut(s) 67, 441, 1329, 1433
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 1 cut(s) 513
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 5 cut(s) 261, 1029, 1044, 1386, 1666
BsoBI CYCGRG 1 cut(s) 1436
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
Bsp1720I GCTNAGC 1 cut(s) 349
BspACI CCGC 3 cut(s) 863, 1146, 1485
BspANI GGCC 5 cut(s) 261, 1029, 1044, 1386, 1666
BspCNI CTCAG 5 cut(s) 362, 1228, 1297, 1420, 1552
BspDI ATCGAT 1 cut(s) 1346
BspFNI CGCG 1 cut(s) 516
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 156, 861
BspMAI CTGCAG 3 cut(s) 135, 1093, 1657
BspPI GGATC 3 cut(s) 854, 867, 1274
BspT104I TTCGAA 1 cut(s) 166
BspTI CTTAAG 1 cut(s) 1604
BsrDI GCAATG 1 cut(s) 1253
BsrFI RCCGGY 1 cut(s) 739
BsrI ACTGG 3 cut(s) 544, 1127, 1169
BssAI RCCGGY 1 cut(s) 739
BssMI GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
Bst4CI ACNGT 4 cut(s) 829, 922, 1261, 1517
Bst6I CTCTTC 1 cut(s) 177
BstAFI CTTAAG 1 cut(s) 1604
BstAPI GCANNNNNTGC 1 cut(s) 1459
BstBAI YACGTR 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 651, 930, 1196
BstDEI CTNAG 8 cut(s) 349, 872, 946, 1176, 1215, 1284, 1428, 1539
BstF5I GGATG 3 cut(s) 666, 1507, 1604
BstFNI CGCG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 2 cut(s) 793, 1580
BstKTI GATC 7 cut(s) 236, 706, 862, 979, 1282, 1528, 1683
BstMAI GTCTC 4 cut(s) 67, 441, 1329, 1433
BstMBI GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
BstMWI GCNNNNNNNGC 2 cut(s) 1459, 1586
BstNSI RCATGY 3 cut(s) 50, 554, 1453
BstPAI GACNNNNGTC 1 cut(s) 941
BstSFI CTRYAG 6 cut(s) 131, 237, 842, 1089, 1590, 1653
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 3 cut(s) 1243, 1603, 1639
BstV2I GAAGAC 4 cut(s) 468, 840, 1047, 1112
BstX2I RGATCY 3 cut(s) 233, 859, 1279
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 3 cut(s) 233, 859, 1279
Bsu15I ATCGAT 1 cut(s) 1346
BsuI GTATCC 1 cut(s) 1311
BsuRI GGCC 5 cut(s) 261, 1029, 1044, 1386, 1666
BsuTUI ATCGAT 1 cut(s) 1346
BtsCI GGATG 3 cut(s) 666, 1507, 1604
BtsIMutI CAGTG 3 cut(s) 184, 597, 1528
Cac8I GCNNGC 3 cut(s) 651, 930, 1196
CaiI CAGNNNCTG 1 cut(s) 1291
CciI TCATGA 1 cut(s) 852
CfoI GCGC 2 cut(s) 793, 1580
Cfr10I RCCGGY 1 cut(s) 739
ClaI ATCGAT 1 cut(s) 1346
Csp6I GTAC 1 cut(s) 1531
CspAI ACCGGT 1 cut(s) 739
CviAII CATG 7 cut(s) 29, 47, 551, 749, 853, 1073, 1450
CviQI GTAC 1 cut(s) 1531
DdeI CTNAG 8 cut(s) 349, 872, 946, 1176, 1215, 1284, 1428, 1539
DpnI GATC 7 cut(s) 235, 705, 861, 978, 1281, 1527, 1682
DpnII GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco147I AGGCCT 4 cut(s) 261, 1029, 1386, 1666
Eco32I GATATC 1 cut(s) 1479
Eco47III AGCGCT 1 cut(s) 792
Eco57I CTGAAG 1 cut(s) 1344
Eco72I CACGTG 1 cut(s) 361
Eco88I CYCGRG 1 cut(s) 1436
EcoRV GATATC 1 cut(s) 1479
Esp3I CGTCTC 1 cut(s) 1433
FaeI CATG 7 cut(s) 32, 50, 554, 752, 856, 1076, 1453
FaqI GGGAC 1 cut(s) 513
FatI CATG 7 cut(s) 28, 46, 550, 748, 852, 1072, 1449
FauI CCCGC 1 cut(s) 1478
FbaI TGATCA 1 cut(s) 1525
Fnu4HI GCNGC 3 cut(s) 1232, 1617, 1653
FokI GGATG 3 cut(s) 653, 1514, 1611
Fsp4HI GCNGC 3 cut(s) 1232, 1617, 1653
GlaI GCGC 2 cut(s) 792, 1579
GluI GCNGC 3 cut(s) 1232, 1617, 1653
GsuI CTGGAG 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 5 cut(s) 261, 1029, 1044, 1386, 1666
HapII CCGG 2 cut(s) 384, 740
HhaI GCGC 2 cut(s) 793, 1580
Hin1II CATG 7 cut(s) 32, 50, 554, 752, 856, 1076, 1453
Hin6I GCGC 2 cut(s) 791, 1578
HinP1I GCGC 2 cut(s) 791, 1578
HindIII AAGCTT 3 cut(s) 80, 877, 1192
HinfI GANTC 5 cut(s) 65, 253, 410, 1061, 1505
HpaII CCGG 2 cut(s) 384, 740
HphI GGTGA 2 cut(s) 554, 1114
Hpy188I TCNGA 7 cut(s) 293, 1060, 1218, 1279, 1293, 1429, 1542
Hpy188III TCNNGA 8 cut(s) 41, 231, 535, 760, 853, 1156, 1438, 1465
HpyAV CCTTC 7 cut(s) 502, 512, 560, 850, 1174, 1231, 1559
HpyCH4III ACNGT 4 cut(s) 829, 922, 1261, 1517
HpyCH4IV ACGT 3 cut(s) 193, 360, 526
HpyF10VI GCNNNNNNNGC 2 cut(s) 1459, 1586
HpyF3I CTNAG 8 cut(s) 349, 872, 946, 1176, 1215, 1284, 1428, 1539
HpySE526I ACGT 3 cut(s) 193, 360, 526
Hsp92II CATG 7 cut(s) 32, 50, 554, 752, 856, 1076, 1453
HspAI GCGC 2 cut(s) 791, 1578
Ksp22I TGATCA 1 cut(s) 1525
Kzo9I GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
LmnI GCTCC 2 cut(s) 621, 788
Lsp1109I GCAGC 3 cut(s) 1243, 1603, 1639
LweI GCATC 3 cut(s) 364, 1297, 1422
MaeII ACGT 3 cut(s) 193, 360, 526
MaeIII GTNAC 4 cut(s) 542, 823, 1329, 1493
MalI GATC 7 cut(s) 235, 705, 861, 978, 1281, 1527, 1682
MboI GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
MflI RGATCY 3 cut(s) 233, 859, 1279
MhlI GDGCHC 1 cut(s) 930
MlsI TGGCCA 1 cut(s) 1044
MluCI AATT 9 cut(s) 7, 171, 558, 604, 1019, 1032, 1159, 1410, 1671
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 1 cut(s) 1514
MmeI TCCRAC 2 cut(s) 189, 1336
Mox20I TGGCCA 1 cut(s) 1044
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 6 cut(s) 561, 645, 699, 1005, 1068, 1605
MslI CAYNNNNRTG 6 cut(s) 359, 669, 1014, 1100, 1346, 1624
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 1 cut(s) 995
MspCI CTTAAG 1 cut(s) 1604
MspI CCGG 2 cut(s) 384, 740
Mva1269I GAATGC 1 cut(s) 306
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 1459, 1586
NdeII GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
NlaIII CATG 7 cut(s) 32, 50, 554, 752, 856, 1076, 1453
NlaIV GGNNCC 2 cut(s) 156, 861
NmuCI GTSAC 2 cut(s) 542, 823
NspI RCATGY 3 cut(s) 50, 554, 1453
NspV TTCGAA 1 cut(s) 166
OliI CACNNNNGTG 2 cut(s) 669, 1100
PaeR7I CTCGAG 1 cut(s) 1436
PagI TCATGA 1 cut(s) 852
PceI AGGCCT 4 cut(s) 261, 1029, 1386, 1666
PciI ACATGT 1 cut(s) 550
PctI GAATGC 1 cut(s) 306
PfeI GAWTC 4 cut(s) 65, 253, 410, 1061
PinAI ACCGGT 1 cut(s) 739
PkrI GCNGC 3 cut(s) 1233, 1618, 1654
PleI GAGTC 1 cut(s) 1513
PmaCI CACGTG 1 cut(s) 361
PmlI CACGTG 1 cut(s) 361
PpsI GAGTC 1 cut(s) 1513
Ppu21I YACGTR 1 cut(s) 361
PscI ACATGT 1 cut(s) 550
PshAI GACNNNNGTC 1 cut(s) 941
PspCI CACGTG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 156, 861
PstI CTGCAG 3 cut(s) 135, 1093, 1657
PstNI CAGNNNCTG 1 cut(s) 1291
PsuI RGATCY 3 cut(s) 233, 859, 1279
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 1 cut(s) 1532
RsaNI GTAC 1 cut(s) 1531
RseI CAYNNNNRTG 6 cut(s) 359, 669, 1014, 1100, 1346, 1624
SaqAI TTAA 6 cut(s) 561, 645, 699, 1005, 1068, 1605
SatI GCNGC 3 cut(s) 1232, 1617, 1653
Sau3AI GATC 7 cut(s) 233, 703, 859, 976, 1279, 1525, 1680
SchI GAGTC 1 cut(s) 1514
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 3 cut(s) 364, 1297, 1422
SfcI CTRYAG 6 cut(s) 131, 237, 842, 1089, 1590, 1653
Sfr274I CTCGAG 1 cut(s) 1436
SfuI TTCGAA 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 1436
SmiMI CAYNNNNRTG 6 cut(s) 359, 669, 1014, 1100, 1346, 1624
SmlI CTYRAG 3 cut(s) 1436, 1463, 1604
SmoI CTYRAG 3 cut(s) 1436, 1463, 1604
Sse9I AATT 9 cut(s) 7, 171, 558, 604, 1019, 1032, 1159, 1410, 1671
SseBI AGGCCT 4 cut(s) 261, 1029, 1386, 1666
SsiI CCGC 3 cut(s) 863, 1146, 1485
SspI AATATT 1 cut(s) 430
StuI AGGCCT 4 cut(s) 261, 1029, 1386, 1666
TaaI ACNGT 4 cut(s) 829, 922, 1261, 1517
TaiI ACGT 3 cut(s) 196, 363, 529
TaqI TCGA 9 cut(s) 166, 218, 264, 333, 608, 1346, 1414, 1437, 1683
TasI AATT 9 cut(s) 7, 171, 558, 604, 1019, 1032, 1159, 1410, 1671
TfiI GAWTC 4 cut(s) 65, 253, 410, 1061
Tru1I TTAA 6 cut(s) 561, 645, 699, 1005, 1068, 1605
Tru9I TTAA 6 cut(s) 561, 645, 699, 1005, 1068, 1605
TscAI CASTG 3 cut(s) 184, 604, 1528
TseFI GTSAC 2 cut(s) 542, 823
TseI GCWGC 3 cut(s) 1231, 1616, 1652
Tsp45I GTSAC 2 cut(s) 542, 823
TspDTI ATGAA 5 cut(s) 29, 474, 768, 869, 1032
TspGWI ACGGA 3 cut(s) 659, 1418, 1457
TspRI CASTG 3 cut(s) 184, 604, 1528
Vha464I CTTAAG 1 cut(s) 1604
XapI RAATTY 3 cut(s) 7, 1019, 1410
XceI RCATGY 3 cut(s) 50, 554, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.