Rh2DG017600

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004372
Physical Location & Seq
Forward (+)
1012197 .. 1016120
3924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG017600.1

Sequence Viewer

Length: 1485 bp
ATGCCACCACATCTATTCACAGGTGCAAAGGTTGAGGGAGAGCAAGGAGCAGCAATTCATGTTGTGTTGCTAGACTTAAGTAAAGGCTCTGTTGTACAAACAGGACCAGAAGCATCTGCAAAGCTCAATATTGTTGTACTGGAAGGTGACTTCAATGAAGAAGTTGAGGATGGTTGGACCAAAGAACATTTTGAAAACCATGAGGTAAAGGAGCGTGAGGGGAAAAGACCACTTCTGACAGGGGATGTACAATTTATTCTTAAGGAAGGGGTGGGTACACTTGGAGAGCTTACTTTTACAGACAATTCTAGCTGGATAAGGAGTAGGAAATTTAGGCTTGGAGTTAAGGTTGCCCCTGGAAACTGTGAAGGAATTCGTATTTGTGAAGCTAAAACAGATGCTTTTTTGGTTAAAGATCACAGGGGAGAATTATACAAGAAACATTACCCACCGGCTTTGCATGATGAGGTGTGGAGATTAGATAGAATAGCAAAGGATGGAGCTCTACACAAGAAGCTGATTAAGTCTGAGATTGTAACTGTTGAAGACTTTCTCCGTCTTCTTGTTAAGGACCCGCAGAAATTAAGAAGTATCCTTGGGAGCGGTATGTCCAACAGGATGTGGGAAAACACCGTGGAGCATGCAAAAACTTGTGTCTTGAGTGGAAAGCTCTATGTTTACTACACTGATGAAACTCAAACCAAGGGTGTTGTATTCAACCATATTTATGAACTCAGGGGCCTCATTGCTGATGGGCAATTTTTCTCCCTAGAATCCCTTACTCACAGTCAAAAGGTTTCAGTGGATACTCTGATGAAGAGAGCATATGATAATTGGCATCAAGTCATAGAATACAATGGCATGATGACTGCACCTGTGGCAGTCGCTTCCCACAACAATGCCGTAGATCACCATTCTGCAGCTATTCAAGGCAGGCCGCAATATAACTCTGATTTCGGTCAACAGTTTCGAATAGAAAATAACCACCCACCAGTCGTCCCTCAGTTTGTTCCATTTGTGAGGTCTGATCAAACATCTTTGGTTAAATTAAACAACCCACAAACAGCTTTACCTAGCACCACGGGTTACATGTCACTTGGAGGTACATCAAATTTTTCAACAGATTGGTCACGGCCAAATTATGGGAACGGGTTTGAGGACTTTAATGTTGCTGATGAAATCCGGATCAGGAGTTCAGAAATGTTGGAGAGTGATGACATGCAGAGACTGTTAATGAAGACATTTAGTATGGGAGTTGGAATCGGAACAGGACCTAGTTTTGGTAATTCTGATGAAGCTTGTTATAATTATAGTGTACAGTATGAGCCTCATGTAGGTCAATCAGTGGGGAAAGAGCATGGAAAAAGTTCAGGAAAAGCTGTTGTTGGATGGCTTAAGCTCAAAGCTGCTTTAAGGTGGGGAATATTTGTTAGGAAGAGAGCTGCAGAACGGAGAGCTCAGCTTACACTTACAGAACTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

494

Amino Acids

55.33

Weight (kDa)

6.54

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 2 - 140 5.7e-55 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 152 - 218 5.6e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 223 - 285 6.6e-22 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
AccB7I CCANNNNNTGG 1 cut(s) 1142
AccBSI CCGCTC 1 cut(s) 603
AccIII TCCGGA 1 cut(s) 1182
AciI CCGC 3 cut(s) 575, 603, 938
AclWI GGATC 1 cut(s) 1193
AcoI YGGCCR 1 cut(s) 1133
AcsI RAATTY 3 cut(s) 329, 372, 1111
AfaI GTAC 6 cut(s) 96, 138, 249, 277, 1105, 1317
AfiI CCNNNNNNNGG 3 cut(s) 1142, 1280, 1334
AflII CTTAAG 3 cut(s) 76, 260, 1394
AflIII ACRYGT 1 cut(s) 1089
AgsI TTSAA 6 cut(s) 154, 194, 545, 718, 929, 1119
AjnI CCWGG 1 cut(s) 355
AloI GAACNNNNNNTCC 2 cut(s) 1177, 1209
Alw21I GWGCWC 2 cut(s) 505, 1459
Alw26I GTCTC 1 cut(s) 1219
AlwI GGATC 1 cut(s) 1193
AlwNI CAGNNNCTG 1 cut(s) 1228
Aor13HI TCCGGA 1 cut(s) 1182
AoxI GGCC 3 cut(s) 739, 935, 1133
ApeKI GCWGC 4 cut(s) 50, 920, 1406, 1442
ApoI RAATTY 3 cut(s) 329, 372, 1111
Asp700I GAANNNNTTC 3 cut(s) 372, 549, 1366
AspS9I GGNCC 5 cut(s) 104, 177, 571, 739, 1271
AsuHPI GGTGA 2 cut(s) 158, 902
AsuII TTCGAA 1 cut(s) 970
AvaII GGWCC 4 cut(s) 104, 177, 571, 1271
BanII GRGCYC 2 cut(s) 505, 1459
BbsI GAAGAC 3 cut(s) 551, 552, 1244
Bbv12I GWGCWC 2 cut(s) 505, 1459
BbvI GCAGC 4 cut(s) 62, 932, 1393, 1429
BccI CCATC 4 cut(s) 164, 491, 746, 1383
BceAI ACGGC 2 cut(s) 887, 1148
BciT130I CCWGG 1 cut(s) 357
BciVI GTATCC 2 cut(s) 602, 799
BclI TGATCA 1 cut(s) 1027
BcoDI GTCTC 1 cut(s) 1219
BfaI CTAG 6 cut(s) 71, 309, 770, 1074, 1275, 1478
BfmI CTRYAG 2 cut(s) 918, 1443
BfrI CTTAAG 3 cut(s) 76, 260, 1394
BfuI GTATCC 2 cut(s) 602, 799
BisI GCNGC 5 cut(s) 51, 921, 938, 1407, 1443
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 5 cut(s) 52, 922, 939, 1408, 1444
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 4 cut(s) 104, 177, 571, 1271
BmgT120I GGNCC 5 cut(s) 104, 177, 571, 739, 1271
BmiI GGNNCC 2 cut(s) 573, 740
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 3 cut(s) 122, 388, 847
BpiI GAAGAC 3 cut(s) 551, 552, 1244
Bpu1102I GCTNAGC 1 cut(s) 1458
Bpu14I TTCGAA 1 cut(s) 970
BpuEI CTTGAG 1 cut(s) 679
BsaJI CCNNGG 5 cut(s) 355, 595, 633, 702, 1080
BsaWI WCCGGW 1 cut(s) 1182
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 1142, 1280, 1334
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 144, 992
Bse3DI GCAATG 1 cut(s) 744
BseAI TCCGGA 1 cut(s) 1182
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 5 cut(s) 355, 595, 633, 702, 1080
BseGI GGATG 5 cut(s) 175, 250, 502, 624, 1394
BseLI CCNNNNNNNGG 3 cut(s) 1142, 1280, 1334
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 4 cut(s) 519, 748, 1016, 1472
BseNI ACTGG 2 cut(s) 144, 992
BseXI GCAGC 4 cut(s) 62, 932, 1393, 1429
BsgI GTGCAG 1 cut(s) 855
BshFI GGCC 3 cut(s) 741, 937, 1135
BsiHKAI GWGCWC 2 cut(s) 505, 1459
BsiSI CCGG 2 cut(s) 452, 1183
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 3 cut(s) 1142, 1280, 1334
BsmAI GTCTC 1 cut(s) 1219
BsmFI GGGAC 1 cut(s) 983
BsnI GGCC 3 cut(s) 741, 937, 1135
Bsp119I TTCGAA 1 cut(s) 970
Bsp1286I GDGCHC 2 cut(s) 505, 1459
Bsp13I TCCGGA 1 cut(s) 1182
Bsp1407I TGTACA 3 cut(s) 94, 247, 1315
Bsp143I GATC 4 cut(s) 415, 907, 1027, 1185
Bsp1720I GCTNAGC 1 cut(s) 1458
BspACI CCGC 3 cut(s) 575, 603, 938
BspANI GGCC 3 cut(s) 741, 937, 1135
BspCNI CTCAG 4 cut(s) 520, 747, 1015, 1471
BspEI TCCGGA 1 cut(s) 1182
BspLI GGNNCC 2 cut(s) 573, 740
BspMAI CTGCAG 2 cut(s) 922, 1447
BspPI GGATC 1 cut(s) 1193
BspT104I TTCGAA 1 cut(s) 970
BspTI CTTAAG 3 cut(s) 76, 260, 1394
BsrBI CCGCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 451
BsrGI TGTACA 3 cut(s) 94, 247, 1315
BsrI ACTGG 2 cut(s) 144, 992
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 5 cut(s) 355, 595, 633, 702, 1080
BssMI GATC 4 cut(s) 415, 907, 1027, 1185
BssT1I CCWWGG 2 cut(s) 595, 702
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 7 cut(s) 365, 541, 634, 788, 966, 1230, 1320
Bst6I CTCTTC 2 cut(s) 812, 1430
BstAFI CTTAAG 3 cut(s) 76, 260, 1394
BstAUI TGTACA 3 cut(s) 94, 247, 1315
BstBI TTCGAA 1 cut(s) 970
BstC8I GCNNGC 2 cut(s) 642, 935
BstDEI CTNAG 4 cut(s) 528, 734, 1002, 1458
BstDSI CCRYGG 2 cut(s) 633, 1080
BstENI CCTNNNNNAGG 1 cut(s) 1332
BstF5I GGATG 5 cut(s) 175, 250, 502, 624, 1394
BstKTI GATC 4 cut(s) 418, 910, 1030, 1188
BstMAI GTCTC 1 cut(s) 1219
BstMBI GATC 4 cut(s) 415, 907, 1027, 1185
BstMWI GCNNNNNNNGC 1 cut(s) 878
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 3 cut(s) 644, 1093, 1222
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 2 cut(s) 918, 1443
BstV1I GCAGC 4 cut(s) 62, 932, 1393, 1429
BstV2I GAAGAC 3 cut(s) 551, 552, 1244
BsuI GTATCC 2 cut(s) 602, 799
BsuRI GGCC 3 cut(s) 741, 937, 1135
BtgI CCRYGG 2 cut(s) 633, 1080
BtsCI GGATG 5 cut(s) 175, 250, 502, 624, 1394
BtsIMutI CAGTG 3 cut(s) 684, 807, 1350
Cac8I GCNNGC 2 cut(s) 642, 935
CaiI CAGNNNCTG 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 5 cut(s) 104, 177, 571, 739, 1271
Csp6I GTAC 6 cut(s) 95, 137, 248, 276, 1104, 1316
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 9 cut(s) 59, 200, 461, 641, 862, 1090, 1219, 1331, 1358
CviQI GTAC 6 cut(s) 95, 137, 248, 276, 1104, 1316
DdeI CTNAG 4 cut(s) 528, 734, 1002, 1458
DpnI GATC 4 cut(s) 417, 909, 1029, 1187
DpnII GATC 4 cut(s) 415, 907, 1027, 1185
EaeI YGGCCR 1 cut(s) 1133
Eam1104I CTCTTC 2 cut(s) 812, 1430
EarI CTCTTC 2 cut(s) 812, 1430
Ecl136II GAGCTC 2 cut(s) 503, 1457
Eco130I CCWWGG 2 cut(s) 595, 702
Eco24I GRGCYC 2 cut(s) 505, 1459
Eco47I GGWCC 4 cut(s) 104, 177, 571, 1271
Eco53kI GAGCTC 2 cut(s) 503, 1457
EcoICRI GAGCTC 2 cut(s) 503, 1457
EcoNI CCTNNNNNAGG 1 cut(s) 1332
EcoO109I RGGNCCY 3 cut(s) 571, 739, 1271
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 595, 702
EcoT38I GRGCYC 2 cut(s) 505, 1459
ErhI CCWWGG 2 cut(s) 595, 702
FaeI CATG 9 cut(s) 62, 203, 464, 644, 865, 1093, 1222, 1334, 1361
FaqI GGGAC 1 cut(s) 983
FatI CATG 9 cut(s) 58, 199, 460, 640, 861, 1089, 1218, 1330, 1357
FauI CCCGC 1 cut(s) 582
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 1027
Fnu4HI GCNGC 5 cut(s) 51, 921, 938, 1407, 1443
FokI GGATG 5 cut(s) 182, 257, 509, 631, 1401
FriOI GRGCYC 2 cut(s) 505, 1459
Fsp4HI GCNGC 5 cut(s) 51, 921, 938, 1407, 1443
FspBI CTAG 6 cut(s) 71, 309, 770, 1074, 1275, 1478
GluI GCNGC 5 cut(s) 51, 921, 938, 1407, 1443
HaeIII GGCC 3 cut(s) 741, 937, 1135
HapII CCGG 2 cut(s) 452, 1183
Hin1II CATG 9 cut(s) 62, 203, 464, 644, 865, 1093, 1222, 1334, 1361
HincII GTYRAC 1 cut(s) 962
HindII GTYRAC 1 cut(s) 962
HindIII AAGCTT 1 cut(s) 1296
HinfI GANTC 2 cut(s) 773, 1260
HpaII CCGG 2 cut(s) 452, 1183
HphI GGTGA 2 cut(s) 158, 902
Hpy166II GTNNAC 4 cut(s) 278, 679, 962, 1316
Hpy188I TCNGA 8 cut(s) 237, 529, 813, 952, 1027, 1198, 1265, 1291
Hpy188III TCNNGA 4 cut(s) 658, 1183, 1189, 1371
Hpy8I GTNNAC 4 cut(s) 278, 679, 962, 1316
HpyAV CCTTC 3 cut(s) 137, 260, 362
HpyCH4III ACNGT 7 cut(s) 365, 541, 634, 788, 966, 1230, 1320
HpyCH4V TGCA 8 cut(s) 26, 119, 460, 644, 872, 920, 1222, 1445
HpyF10VI GCNNNNNNNGC 1 cut(s) 878
HpyF3I CTNAG 4 cut(s) 528, 734, 1002, 1458
Hsp92II CATG 9 cut(s) 62, 203, 464, 644, 865, 1093, 1222, 1334, 1361
Kpn2I TCCGGA 1 cut(s) 1182
Ksp22I TGATCA 1 cut(s) 1027
Kzo9I GATC 4 cut(s) 415, 907, 1027, 1185
LmnI GCTCC 5 cut(s) 47, 211, 500, 600, 637
Lsp1109I GCAGC 4 cut(s) 62, 932, 1393, 1429
LweI GCATC 3 cut(s) 122, 388, 847
MaeI CTAG 6 cut(s) 71, 309, 770, 1074, 1275, 1478
MaeIII GTNAC 5 cut(s) 146, 535, 1085, 1092, 1128
MalI GATC 4 cut(s) 417, 909, 1029, 1187
MbiI CCGCTC 1 cut(s) 603
MboI GATC 4 cut(s) 415, 907, 1027, 1185
MboII GAAGA 6 cut(s) 170, 551, 557, 829, 1249, 1447
MhlI GDGCHC 2 cut(s) 505, 1459
MmeI TCCRAC 5 cut(s) 155, 636, 1185, 1237, 1366
MroI TCCGGA 1 cut(s) 1182
MroXI GAANNNNTTC 3 cut(s) 372, 549, 1366
MslI CAYNNNNRTG 2 cut(s) 726, 897
MspCI CTTAAG 3 cut(s) 76, 260, 1394
MspI CCGG 2 cut(s) 452, 1183
MspR9I CCNGG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 878
NdeI CATATG 1 cut(s) 826
NdeII GATC 4 cut(s) 415, 907, 1027, 1185
NlaIII CATG 9 cut(s) 62, 203, 464, 644, 865, 1093, 1222, 1334, 1361
NlaIV GGNNCC 2 cut(s) 573, 740
NmuCI GTSAC 3 cut(s) 146, 1092, 1128
NspI RCATGY 3 cut(s) 644, 1093, 1222
NspV TTCGAA 1 cut(s) 970
PaeI GCATGC 1 cut(s) 644
PciI ACATGT 1 cut(s) 1089
PdmI GAANNNNTTC 3 cut(s) 372, 549, 1366
PfeI GAWTC 2 cut(s) 773, 1260
PflMI CCANNNNNTGG 1 cut(s) 1142
PkrI GCNGC 5 cut(s) 52, 922, 939, 1408, 1444
PpuMI RGGWCCY 2 cut(s) 571, 1271
PscI ACATGT 1 cut(s) 1089
PsiI TTATAA 1 cut(s) 1305
Psp124BI GAGCTC 2 cut(s) 505, 1459
Psp5II RGGWCCY 2 cut(s) 571, 1271
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 573, 740
PspPI GGNCC 5 cut(s) 104, 177, 571, 739, 1271
PspPPI RGGWCCY 2 cut(s) 571, 1271
PstI CTGCAG 2 cut(s) 922, 1447
PstNI CAGNNNCTG 1 cut(s) 1228
RsaI GTAC 6 cut(s) 96, 138, 249, 277, 1105, 1317
RsaNI GTAC 6 cut(s) 95, 137, 248, 276, 1104, 1316
RseI CAYNNNNRTG 2 cut(s) 726, 897
SacI GAGCTC 2 cut(s) 505, 1459
SatI GCNGC 5 cut(s) 51, 921, 938, 1407, 1443
Sau3AI GATC 4 cut(s) 415, 907, 1027, 1185
Sau96I GGNCC 5 cut(s) 104, 177, 571, 739, 1271
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 505, 1459
SfaNI GCATC 3 cut(s) 122, 388, 847
SfcI CTRYAG 2 cut(s) 918, 1443
SfuI TTCGAA 1 cut(s) 970
SinI GGWCC 4 cut(s) 104, 177, 571, 1271
SmiMI CAYNNNNRTG 2 cut(s) 726, 897
SmlI CTYRAG 4 cut(s) 76, 260, 658, 1394
SmoI CTYRAG 4 cut(s) 76, 260, 658, 1394
SphI GCATGC 1 cut(s) 644
SsiI CCGC 3 cut(s) 575, 603, 938
SspI AATATT 2 cut(s) 130, 1425
SspMI CTAG 6 cut(s) 71, 309, 770, 1074, 1275, 1478
SstI GAGCTC 2 cut(s) 505, 1459
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 2 cut(s) 595, 702
TaaI ACNGT 7 cut(s) 365, 541, 634, 788, 966, 1230, 1320
TaqI TCGA 1 cut(s) 970
TaqII GACCGA 1 cut(s) 947
TatI WGTACW 4 cut(s) 94, 136, 247, 1315
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 2 cut(s) 773, 1260
TscAI CASTG 3 cut(s) 691, 807, 1350
TseFI GTSAC 3 cut(s) 146, 1092, 1128
TseI GCWGC 4 cut(s) 50, 920, 1406, 1442
Tsp45I GTSAC 3 cut(s) 146, 1092, 1128
TspDTI ATGAA 8 cut(s) 47, 171, 705, 744, 830, 1191, 1250, 1308
TspGWI ACGGA 2 cut(s) 545, 1465
TspRI CASTG 3 cut(s) 691, 807, 1350
Van91I CCANNNNNTGG 1 cut(s) 1142
Vha464I CTTAAG 3 cut(s) 76, 260, 1394
VpaK11BI GGWCC 4 cut(s) 104, 177, 571, 1271
XagI CCTNNNNNAGG 1 cut(s) 1332
XapI RAATTY 3 cut(s) 329, 372, 1111
XceI RCATGY 3 cut(s) 644, 1093, 1222
XmnI GAANNNNTTC 3 cut(s) 372, 549, 1366
XspI CTAG 6 cut(s) 71, 309, 770, 1074, 1275, 1478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.