AT4G25830

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016020
Physical Location & Seq
Forward (+)
13133402 .. 13135286
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25830.1

Sequence Viewer

Length: 528 bp
ATGGTGAAACTGAGAGAAACAGAAGTGATCCTGAGACTCTGCATCGTCTTTTTTCTTCTCCTCACTTCTTGTTTGATCGGTTTGGATTCTCAGACCAAAGAGATTGCTTATATCCACAAGAACGTCAGTTTCAGATATTTGTTAGCTCTCGAGGCTGAGTTATACATAGATGTTGTGGTTGCTGCGTATAATTTGGTTCAACTAGGGCTTGGTTGGTACAATGTTGAACAAAAGACCTCCAATCCCAAATGGTTCTCTTACCTCTTGGATCAGACGGCGGCATACGTGGTTTTCGCCGGGACATCAGCCGCGGCGCAACACTCATTGCTAGTGGTGACGGGTTCAAGGGAGCTTCAGTGGATGAAATGGTGCTACAAGTTCACAAGGTTCTGTTTTCAGATGGGAAGTGCCATCATCTTAAACTACATTGCGGCAGCTCTCATGGTCCTCCTCTCTTCCATCTCCGCCTTCAACCTCTTCCGTCTCTATTCCCCTAAACGTTTTTTTCGCTTTAAGTCATCCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

175

Amino Acids

20.09

Weight (kDa)

9.32

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 3 - 146 2.7e-36 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25830
fragaria_vesca FvH4_4g09300
malus_domestica MD13G1269300.v1.1
prunus_persica Prupe.1G088100_v2.0.a1
pyrus_communis pycom13g22330
rosa_chinensis RchiOBHm_Chr4g0402761
rosa_laevigata RLG00000009048
rosa_multiflora Rmu_sc0002451.1_g000031
rosa_roxburghii Rroxscaffold_5G00347520
rosa_rugosa Rorug04G0038600
rosa_samantha Rh4AG116000 Rh4BG108600 Rh4CG123300 Rh4DG108800
rosa_wichuraiana Rw4G009280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AciI CCGC 5 cut(s) 278, 309, 311, 431, 465
AclI AACGTT 1 cut(s) 499
AclWI GGATC 2 cut(s) 22, 276
AcuI CTGAAG 1 cut(s) 338
AfaI GTAC 1 cut(s) 218
AgsI TTSAA 4 cut(s) 200, 227, 345, 472
AluBI AGCT 3 cut(s) 146, 352, 437
AluI AGCT 3 cut(s) 146, 352, 437
Alw26I GTCTC 2 cut(s) 28, 488
AlwI GGATC 2 cut(s) 22, 276
Ama87I CYCGRG 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 182, 434
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 1 cut(s) 445
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 2 cut(s) 16, 346
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 169, 446
BccI CCATC 3 cut(s) 394, 419, 467
BceAI ACGGC 1 cut(s) 291
BcnI CCSGG 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 28, 488
BfaI CTAG 2 cut(s) 203, 329
BisI GCNGC 6 cut(s) 183, 279, 309, 312, 432, 435
BlsI GCNGC 6 cut(s) 184, 280, 310, 313, 433, 436
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 298
BsaAI YACGTR 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 309
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bse3DI GCAATG 2 cut(s) 323, 426
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 2 cut(s) 366, 518
BseMI GCAATG 2 cut(s) 323, 426
BseMII CTCAG 3 cut(s) 23, 104, 147
BseRI GAGGAG 2 cut(s) 50, 440
BseXI GCAGC 2 cut(s) 169, 446
Bsh1236I CGCG 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 28, 488
BsmBI CGTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 313
BsoBI CYCGRG 1 cut(s) 149
Bsp143I GATC 3 cut(s) 27, 75, 268
BspACI CCGC 5 cut(s) 278, 309, 311, 431, 465
BspCNI CTCAG 3 cut(s) 24, 103, 148
BspFNI CGCG 1 cut(s) 311
BspPI GGATC 2 cut(s) 22, 276
BsrDI GCAATG 2 cut(s) 323, 426
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 3 cut(s) 27, 75, 268
Bst6I CTCTTC 2 cut(s) 460, 482
BstBAI YACGTR 1 cut(s) 286
BstDEI CTNAG 4 cut(s) 11, 32, 90, 156
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 366, 518
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 3 cut(s) 30, 78, 271
BstMAI GTCTC 2 cut(s) 28, 488
BstMBI GATC 3 cut(s) 27, 75, 268
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 296
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 2 cut(s) 169, 446
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 366, 518
BtsIMutI CAGTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 445
Cfr42I CCGCGG 1 cut(s) 312
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 442
CviJI RGCY 6 cut(s) 146, 155, 208, 308, 352, 437
CviKI_1 RGCY 6 cut(s) 146, 155, 208, 308, 352, 437
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 4 cut(s) 11, 32, 90, 156
DpnI GATC 3 cut(s) 29, 77, 270
DpnII GATC 3 cut(s) 27, 75, 268
Eam1104I CTCTTC 2 cut(s) 460, 482
EarI CTCTTC 2 cut(s) 460, 482
EciI GGCGGA 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 1 cut(s) 445
FaiI YATR 6 cut(s) 111, 163, 167, 189, 283, 443
FaqI GGGAC 1 cut(s) 313
FatI CATG 1 cut(s) 441
Fnu4HI GCNGC 6 cut(s) 183, 279, 309, 312, 432, 435
FokI GGATG 2 cut(s) 373, 505
Fsp4HI GCNGC 6 cut(s) 183, 279, 309, 312, 432, 435
FspBI CTAG 2 cut(s) 203, 329
GlaI GCGC 1 cut(s) 315
GluI GCNGC 6 cut(s) 183, 279, 309, 312, 432, 435
HapII CCGG 1 cut(s) 297
HhaI GCGC 1 cut(s) 316
Hin1II CATG 1 cut(s) 445
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HinfI GANTC 2 cut(s) 36, 86
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 16, 346
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 4 cut(s) 93, 134, 273, 399
Hpy188III TCNNGA 2 cut(s) 31, 149
Hpy8I GTNNAC 1 cut(s) 381
HpyAV CCTTC 1 cut(s) 478
HpyCH4IV ACGT 3 cut(s) 123, 285, 499
HpyCH4V TGCA 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 4 cut(s) 11, 32, 90, 156
HpySE526I ACGT 3 cut(s) 123, 285, 499
Hsp92II CATG 1 cut(s) 445
HspAI GCGC 1 cut(s) 314
KspI CCGCGG 1 cut(s) 312
Kzo9I GATC 3 cut(s) 27, 75, 268
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 2 cut(s) 44, 310
Lsp1109I GCAGC 2 cut(s) 169, 446
LweI GCATC 1 cut(s) 51
MaeI CTAG 2 cut(s) 203, 329
MaeII ACGT 3 cut(s) 123, 285, 499
MaeIII GTNAC 1 cut(s) 334
MalI GATC 3 cut(s) 29, 77, 270
MboI GATC 3 cut(s) 27, 75, 268
MboII GAAGA 3 cut(s) 47, 447, 469
MluCI AATT 1 cut(s) 190
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 7 cut(s) 71, 145, 247, 272, 458, 461, 485
MseI TTAA 2 cut(s) 419, 513
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 298
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 152
NciI CCSGG 1 cut(s) 298
NdeII GATC 3 cut(s) 27, 75, 268
NlaIII CATG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 149
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 6 cut(s) 184, 280, 310, 313, 433, 436
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Ppu21I YACGTR 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 499
PspPI GGNCC 1 cut(s) 445
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SacII CCGCGG 1 cut(s) 312
SaqAI TTAA 2 cut(s) 419, 513
SatI GCNGC 6 cut(s) 183, 279, 309, 312, 432, 435
Sau3AI GATC 3 cut(s) 27, 75, 268
Sau96I GGNCC 1 cut(s) 445
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 298
SfaNI GCATC 1 cut(s) 51
Sfr274I CTCGAG 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 312
SgrBI CCGCGG 1 cut(s) 312
SinI GGWCC 1 cut(s) 445
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 1 cut(s) 190
SsiI CCGC 5 cut(s) 278, 309, 311, 431, 465
SspMI CTAG 2 cut(s) 203, 329
StyD4I CCNGG 1 cut(s) 296
TaiI ACGT 3 cut(s) 126, 288, 502
TaqI TCGA 1 cut(s) 150
TasI AATT 1 cut(s) 190
TauI GCSGC 4 cut(s) 281, 311, 314, 434
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 419, 513
Tru9I TTAA 2 cut(s) 419, 513
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 182, 434
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 377
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 149
XspI CTAG 2 cut(s) 203, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.