MD13G1269300.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016020
Physical Location & Seq
Forward (+)
35565241 .. 35568457
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1269300.v1.1.491

Sequence Viewer

Length: 579 bp
ATGAACCAGATTATTCAGTCAGAGAAAATGAAGTATGGCATGGCAGAAACGGAGGCTTTTCTAAGGCTTTCTGCAGTTTTAATACTAGTCCTTACTGCATGTTTAGTAGGATTTGATACCCAAACCAAATATGTAATTTTCTTCTATCGAAAAGCCACCTTCAGAGATTTCAATGCCCTCTTGCTTTTGGTTTATGTAGTTTCGGTGGCTGCGGGTTATAATTTGTTTCAAGTTGGAAAATCTTCTCTGTCAGCTTGCTTCAAACCAAATCTGAAAGTGCGGACTAATATATACCTAGCTTGGATTTGTTTCTTGTTGGATCAGATGGCAGTGTACGTCACATTTGGTGCAAACTCAGCTACGTTCCAGGCTTCGCTTTTAGCAGTAACAGGGCAAGAAGATTTTCAATGGATCAAGTTGTGCAGCAAATATACAAGATTTTGCTTTCAAATTGGTGGGGCTTTGACATGTGGATATGCAGCATGTATCGCAATGGCTTTCATATCCTCCATCAGCGCTTACAACCTCTTTAGGCTTTACTCACCAAAACAATTTCTTCAATTAAAGACTGCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

193

Amino Acids

21.52

Weight (kDa)

9.27

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 15 - 164 4.6e-31 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25830
fragaria_vesca FvH4_4g09300
malus_domestica MD13G1269300.v1.1
prunus_persica Prupe.1G088100_v2.0.a1
pyrus_communis pycom13g22330
rosa_chinensis RchiOBHm_Chr4g0402761
rosa_laevigata RLG00000009048
rosa_multiflora Rmu_sc0002451.1_g000031
rosa_roxburghii Rroxscaffold_5G00347520
rosa_rugosa Rorug04G0038600
rosa_samantha Rh4AG116000 Rh4BG108600 Rh4CG123300 Rh4DG108800
rosa_wichuraiana Rw4G009280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AciI CCGC 2 cut(s) 212, 280
AclWI GGATC 2 cut(s) 327, 419
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 1 cut(s) 335
AfeI AGCGCT 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 6 cut(s) 172, 230, 262, 407, 449, 560
AhlI ACTAGT 1 cut(s) 85
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 3 cut(s) 254, 299, 359
AluI AGCT 3 cut(s) 254, 299, 359
AlwI GGATC 2 cut(s) 327, 419
Aor51HI AGCGCT 1 cut(s) 517
ApeKI GCWGC 3 cut(s) 209, 423, 479
Asp700I GAANNNNTTC 2 cut(s) 241, 402
AspLEI GCGC 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 534
BbvI GCAGC 3 cut(s) 196, 435, 491
BccI CCATC 2 cut(s) 319, 518
BciT130I CCWGG 1 cut(s) 368
BcuI ACTAGT 1 cut(s) 85
BfaI CTAG 2 cut(s) 86, 296
BfmI CTRYAG 2 cut(s) 72, 570
BfoI RGCGCY 1 cut(s) 519
BisI GCNGC 3 cut(s) 210, 424, 480
BlsI GCNGC 3 cut(s) 211, 425, 481
Bme1390I CCNGG 1 cut(s) 368
BmrFI CCNGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 368
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 369
BseXI GCAGC 3 cut(s) 196, 435, 491
BsgI GTGCAG 1 cut(s) 442
Bsp143I GATC 2 cut(s) 319, 411
BspACI CCGC 2 cut(s) 212, 280
BspCNI CTCAG 1 cut(s) 368
BspMAI CTGCAG 2 cut(s) 76, 574
BspPI GGATC 2 cut(s) 327, 419
BsrDI GCAATG 1 cut(s) 498
BssMI GATC 2 cut(s) 319, 411
Bst2UI CCWGG 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 2 cut(s) 62, 355
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 2 cut(s) 322, 414
BstMBI GATC 2 cut(s) 319, 411
BstMWI GCNNNNNNNGC 2 cut(s) 356, 488
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 3 cut(s) 102, 471, 486
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 2 cut(s) 72, 570
BstV1I GCAGC 3 cut(s) 196, 435, 491
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 256
CfoI GCGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 40, 99, 468, 483
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 62, 355
DpnI GATC 2 cut(s) 321, 413
DpnII GATC 2 cut(s) 319, 411
Eco47III AGCGCT 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 43, 102, 471, 486
FatI CATG 4 cut(s) 39, 98, 467, 482
FauI CCCGC 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 210, 424, 480
Fsp4HI GCNGC 3 cut(s) 210, 424, 480
FspBI CTAG 2 cut(s) 86, 296
GlaI GCGC 1 cut(s) 517
GluI GCNGC 3 cut(s) 210, 424, 480
HaeII RGCGCY 1 cut(s) 519
HhaI GCGC 1 cut(s) 518
Hin1II CATG 4 cut(s) 43, 102, 471, 486
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HphI GGTGA 1 cut(s) 534
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 22, 164, 273, 324
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 169
HpyCH4IV ACGT 2 cut(s) 336, 362
HpyCH4V TGCA 6 cut(s) 74, 98, 350, 423, 479, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 488
HpyF3I CTNAG 2 cut(s) 62, 355
HpySE526I ACGT 2 cut(s) 336, 362
Hsp92II CATG 4 cut(s) 43, 102, 471, 486
HspAI GCGC 1 cut(s) 516
Kzo9I GATC 2 cut(s) 319, 411
LpnPI CCDG 5 cut(s) 20, 353, 375, 380, 558
Lsp1109I GCAGC 3 cut(s) 196, 435, 491
MaeI CTAG 2 cut(s) 86, 296
MaeII ACGT 2 cut(s) 336, 362
MaeIII GTNAC 2 cut(s) 337, 385
MalI GATC 2 cut(s) 321, 413
MboI GATC 2 cut(s) 319, 411
MboII GAAGA 4 cut(s) 133, 234, 410, 548
MluCI AATT 5 cut(s) 135, 220, 450, 551, 560
MmeI TCCRAC 2 cut(s) 214, 297
MnlI CCTC 4 cut(s) 46, 188, 517, 536
MroXI GAANNNNTTC 2 cut(s) 241, 402
MseI TTAA 2 cut(s) 80, 563
MspR9I CCNGG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 356, 488
NdeII GATC 2 cut(s) 319, 411
NlaIII CATG 4 cut(s) 43, 102, 471, 486
NmuCI GTSAC 1 cut(s) 337
NspI RCATGY 3 cut(s) 102, 471, 486
PciI ACATGT 1 cut(s) 467
PdmI GAANNNNTTC 2 cut(s) 241, 402
PkrI GCNGC 3 cut(s) 211, 425, 481
PscI ACATGT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PstI CTGCAG 2 cut(s) 76, 574
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 2 cut(s) 80, 563
SatI GCNGC 3 cut(s) 210, 424, 480
Sau3AI GATC 2 cut(s) 319, 411
ScrFI CCNGG 1 cut(s) 368
SetI ASST 8 cut(s) 161, 256, 297, 301, 339, 361, 365, 528
SfcI CTRYAG 2 cut(s) 72, 570
SpeI ACTAGT 1 cut(s) 85
Sse9I AATT 5 cut(s) 135, 220, 450, 551, 560
SsiI CCGC 2 cut(s) 212, 280
SspMI CTAG 2 cut(s) 86, 296
StyD4I CCNGG 1 cut(s) 366
TaiI ACGT 2 cut(s) 339, 365
TaqI TCGA 1 cut(s) 148
TasI AATT 5 cut(s) 135, 220, 450, 551, 560
Tru1I TTAA 2 cut(s) 80, 563
Tru9I TTAA 2 cut(s) 80, 563
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 209, 423, 479
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 17, 44, 490
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 1 cut(s) 336
XceI RCATGY 3 cut(s) 102, 471, 486
XmnI GAANNNNTTC 2 cut(s) 241, 402
XspI CTAG 2 cut(s) 86, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.