FvH4_4g09300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016020
Physical Location & Seq
Forward (+)
10846824 .. 10848248
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09300.t1

Sequence Viewer

Length: 558 bp
ATGAAGTCTCACATTGAGAAAACAGCAGCCTTTCTGAGGCTCTCTTCGGTTTTCCTTTTGGTCCTCACTGCAATACTTGTAGGAGTTGATCATCAGACCAAAATTGTCTTCTTTGTTAAGAAAAAAGCTTCTTTCAACGATGTCAATGCCCTCTTGGTTCTGGTATATGTTGATGCTGTGGTTGCTGGATACAATCTGCTGCGACTGATTGCCAAGTTTCCAATGTCAGCTGGCTTAGTCAAAGAAAATCCAAAAGGGTCCAATGTATACGTAGCTTGGCTTTCTCTCTTGCTCGATCAGATAGCCGTATATGTGGCGTTTGGGGCAAACTCAGCTGCGTTTCAGGCTGCACTGGTAGCACTGAAAGGTATACCAGAATTCCAGTGGATGAAGTTGTGCAACAAATACTCCAGATTCTGCTTTCAAATCGGTGGAGCTTTGTCATGTGGATACTTAGCATGTCTCCTTATGATTCTCATATCTTTCATCACTGCCTTTAAGCTGTTTAGGCTTTACTCACCCAAGAAATTGTTTGTGTTAAAGAGCATATCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

186

Amino Acids

20.4

Weight (kDa)

9.77

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 4 - 156 3.7e-35 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25830
fragaria_vesca FvH4_4g09300
malus_domestica MD13G1269300.v1.1
prunus_persica Prupe.1G088100_v2.0.a1
pyrus_communis pycom13g22330
rosa_chinensis RchiOBHm_Chr4g0402761
rosa_laevigata RLG00000009048
rosa_multiflora Rmu_sc0002451.1_g000031
rosa_roxburghii Rroxscaffold_5G00347520
rosa_rugosa Rorug04G0038600
rosa_samantha Rh4AG116000 Rh4BG108600 Rh4CG123300 Rh4DG108800
rosa_wichuraiana Rw4G009280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 267, 370
AcsI RAATTY 1 cut(s) 377
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 2 cut(s) 136, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 6 cut(s) 128, 230, 275, 335, 437, 502
AluI AGCT 6 cut(s) 128, 230, 275, 335, 437, 502
Alw26I GTCTC 2 cut(s) 12, 467
AlwNI CAGNNNCTG 1 cut(s) 417
ApeKI GCWGC 4 cut(s) 26, 199, 335, 347
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 61, 258
AsuHPI GGTGA 1 cut(s) 510
AvaII GGWCC 2 cut(s) 61, 258
BbsI GAAGAC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 38, 186, 322, 334
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciVI GTATCC 2 cut(s) 182, 443
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 12, 467
BfuI GTATCC 2 cut(s) 182, 443
BisI GCNGC 4 cut(s) 27, 200, 336, 348
BlsI GCNGC 4 cut(s) 28, 201, 337, 349
Bme18I GGWCC 2 cut(s) 61, 258
BmgT120I GGNCC 2 cut(s) 61, 258
BmiI GGNNCC 1 cut(s) 259
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 2 cut(s) 357, 382
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 26, 345
BseNI ACTGG 2 cut(s) 357, 382
BseXI GCAGC 4 cut(s) 38, 186, 322, 334
BsgI GTGCAG 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 2 cut(s) 12, 467
Bsp143I GATC 2 cut(s) 88, 295
BspCNI CTCAG 2 cut(s) 27, 344
BspLI GGNNCC 1 cut(s) 259
BsrI ACTGG 2 cut(s) 357, 382
BssMI GATC 2 cut(s) 88, 295
BssNAI GTATAC 2 cut(s) 268, 371
Bst1107I GTATAC 2 cut(s) 268, 371
Bst6I CTCTTC 1 cut(s) 49
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 4 cut(s) 35, 235, 331, 454
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 393
BstKTI GATC 2 cut(s) 91, 298
BstMAI GTCTC 2 cut(s) 12, 467
BstMBI GATC 2 cut(s) 88, 295
BstMWI GCNNNNNNNGC 6 cut(s) 182, 323, 332, 344, 356, 508
BstNSI RCATGY 1 cut(s) 462
BstSNI TACGTA 1 cut(s) 271
BstV1I GCAGC 4 cut(s) 38, 186, 322, 334
BstV2I GAAGAC 1 cut(s) 100
BstZ17I GTATAC 2 cut(s) 268, 371
BsuI GTATCC 2 cut(s) 182, 443
BtsCI GGATG 1 cut(s) 393
BtsI GCAGTG 2 cut(s) 66, 489
BtsIMutI CAGTG 5 cut(s) 66, 350, 359, 389, 489
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 417
Cfr13I GGNCC 2 cut(s) 61, 258
CviAII CATG 2 cut(s) 444, 459
DdeI CTNAG 4 cut(s) 35, 235, 331, 454
DpnI GATC 2 cut(s) 90, 297
DpnII GATC 2 cut(s) 88, 295
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco105I TACGTA 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 61, 258
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 377
FaeI CATG 2 cut(s) 447, 462
FatI CATG 2 cut(s) 443, 458
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 2 cut(s) 267, 370
Fnu4HI GCNGC 4 cut(s) 27, 200, 336, 348
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 4 cut(s) 27, 200, 336, 348
GluI GCNGC 4 cut(s) 27, 200, 336, 348
GsuI CTGGAG 1 cut(s) 394
Hin1II CATG 2 cut(s) 447, 462
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 2 cut(s) 414, 472
HphI GGTGA 1 cut(s) 510
Hpy166II GTNNAC 2 cut(s) 268, 371
Hpy188I TCNGA 3 cut(s) 36, 96, 300
Hpy188III TCNNGA 1 cut(s) 411
Hpy8I GTNNAC 2 cut(s) 268, 371
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 71, 350, 399
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 323, 332, 344, 356, 508
HpyF3I CTNAG 4 cut(s) 35, 235, 331, 454
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 2 cut(s) 447, 462
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 2 cut(s) 88, 295
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 9 cut(s) 146, 171, 216, 329, 338, 387, 395, 424, 537
Lsp1109I GCAGC 4 cut(s) 38, 186, 322, 334
LweI GCATC 1 cut(s) 163
MaeII ACGT 1 cut(s) 270
MalI GATC 2 cut(s) 90, 297
MboI GATC 2 cut(s) 88, 295
MboII GAAGA 2 cut(s) 36, 100
MluCI AATT 3 cut(s) 102, 377, 527
MnlI CCTC 3 cut(s) 30, 74, 161
MseI TTAA 3 cut(s) 117, 498, 539
MspA1I CMGCKG 2 cut(s) 230, 335
MwoI GCNNNNNNNGC 6 cut(s) 182, 323, 332, 344, 356, 508
NdeII GATC 2 cut(s) 88, 295
NlaIII CATG 2 cut(s) 447, 462
NlaIV GGNNCC 1 cut(s) 259
NspI RCATGY 1 cut(s) 462
PfeI GAWTC 2 cut(s) 414, 472
PkrI GCNGC 4 cut(s) 28, 201, 337, 349
Ppu21I YACGTR 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 2 cut(s) 61, 258
PstNI CAGNNNCTG 1 cut(s) 417
PvuII CAGCTG 2 cut(s) 230, 335
SaqAI TTAA 3 cut(s) 117, 498, 539
SatI GCNGC 4 cut(s) 27, 200, 336, 348
Sau3AI GATC 2 cut(s) 88, 295
Sau96I GGNCC 2 cut(s) 61, 258
SetI ASST 8 cut(s) 130, 232, 273, 277, 337, 370, 439, 504
SfaNI GCATC 1 cut(s) 163
SinI GGWCC 2 cut(s) 61, 258
SnaBI TACGTA 1 cut(s) 271
Sse9I AATT 3 cut(s) 102, 377, 527
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 294
TasI AATT 3 cut(s) 102, 377, 527
TfiI GAWTC 2 cut(s) 414, 472
Tru1I TTAA 3 cut(s) 117, 498, 539
Tru9I TTAA 3 cut(s) 117, 498, 539
TscAI CASTG 5 cut(s) 73, 357, 366, 389, 496
TseI GCWGC 4 cut(s) 26, 199, 335, 347
TspDTI ATGAA 3 cut(s) 17, 404, 475
TspRI CASTG 5 cut(s) 73, 357, 366, 389, 496
VpaK11BI GGWCC 2 cut(s) 61, 258
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 462
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
XmiI GTMKAC 2 cut(s) 267, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.