AT4G27600

carbohydrate kinase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015300
Physical Location & Seq
Reverse (-)
13782213 .. 13785134
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27600.1

Sequence Viewer

Length: 1416 bp
ATGGCGTTCTCCCTCCTCTCTCCAATTCCTACCTCTGCCTCTCTTTTTTTCACTCTCTCCGCCATTACCGCTTCTAATAATGCTTCCTCCTCCTCTCTCTCCCGTCTTTACTCTTTATATCACCCCAAAGGAACCTTACGAACCAGAACCGCGACCTCGTTTTTCGGAGCTTCTGGGAACTTGAGGATTGTTTCCATGGCGGAGAATAGGCAATTGGTTTGTAGGATTGGTGGCGGAGCCGGTGCTGAATTGGATGATATGGATGAAGGTGAAATCCAGAGGATTGGAAATGAAGACGAAGACGACGATGAATTCATACAAGTCCATGCTAATAATTCCTCTGCTGCTTCGCCTGAGAGATGGGATGTTTTGGGTCTCGGCCAAGCCATGGTAGATTTCTCTGGAGTTGTGGATGATGAGTTTCTAAAGAAACTTGGTTTAGAAAAGGGAACAAGGAAACTGATTAATCACGAGGAGAGGGGTAAATTATTACAAGCAATGGATGGTTGTAGCTATAAGGCGGCAGCTGGAGGCTCATTGTCCAACACTTTAGTTGCTCTCGCAAGATTAGGTTCTCAATCCATCTGTGACCGGCCTTTGAATGTGGCAATGGCTGGCAGTATTGGAGGTGACCCTCTCGGTAGCTTTTACGGGACTAAACTACGACGAGCAAATGTAAATTTTCTTTCTGCTCCAATCAAGGACGGAACAACAGGAACAGTGATAGTTCTCACAACTCCTGATGCACAACGTACTATGCTTGCATATCAGGGAACATCCTCTGTCGTTAATTATGATTCTTGTTTGGCTAGTTTGATAGCCAAGACAAATGTCTTTGTTGTGGAAGGCTATTTGTTTGAGCTTCCTGATACTATAAGAACCATAACAAAAGCCTGCGAAGAAGCCCACAGAAACGGGGCACTTGTTGCTGTGACAGCATCAGATGTGTCCTGCATAGAGAGGCATTATGATGATTTCTGGGACATTGTGGGGAACTATGCGGATATTGTATTTGCAAATAGCGATGAAGCAAGAGCGTTTTGTCACTTTTCCGCAGAGGAAAGTCCAATTTCAGCGACAAGATACATGAGCCACTTTGTTCCGTTTGTTTCTGTTACCGATGGAATCAACGGGTCATATATTGGAGTAAAAGGAGAGGCCATCTACATTCCTCCGTCCCCATGCGTGCCAGTTGATACATGTGGTGCTGGAGATGCATATGCTTCAGGGATCTTATACGGTATCTTGAGGGGTGTCTCTGACTTGAAAGGAATGGGAGATATGGCTGCAACGATTGCAGCAACTGTGGTGGGTCAACAAGGAACCAGGCTTAGGGTTCAGGACGCGGTTGAGCTGGCTCGATCACATGCCTTCCGTCTCAATGGTTCTGGTGTTCGAACAGATGTTGGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMMECR1 PfkB

Protein Analysis

471

Amino Acids

50.03

Weight (kDa)

5.08

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 171 - 444 1.7e-36 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 388
AccII CGCG 2 cut(s) 152, 1346
AciI CCGC 9 cut(s) 60, 69, 150, 200, 234, 521, 1001, 1053, 1346
AclWI GGATC 1 cut(s) 1238
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 2 cut(s) 311, 679
AcuI CTGAAG 1 cut(s) 1209
AfaI GTAC 1 cut(s) 754
AfiI CCNNNNNNNGG 2 cut(s) 388, 1332
AflIII ACRYGT 1 cut(s) 1199
AgsI TTSAA 2 cut(s) 601, 1267
AjnI CCWGG 1 cut(s) 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 6 cut(s) 170, 513, 527, 645, 862, 1354
AluI AGCT 6 cut(s) 170, 513, 527, 645, 862, 1354
Alw26I GTCTC 3 cut(s) 380, 1261, 1382
AlwI GGATC 1 cut(s) 1238
AlwNI CAGNNNCTG 1 cut(s) 1304
AoxI GGCC 3 cut(s) 379, 593, 1158
ApeKI GCWGC 4 cut(s) 344, 524, 1286, 1298
ApoI RAATTY 2 cut(s) 311, 679
AseI ATTAAT 1 cut(s) 465
AsuHPI GGTGA 3 cut(s) 113, 281, 641
AsuII TTCGAA 1 cut(s) 1396
BaeGI GKGCMC 1 cut(s) 922
BauI CACGAG 1 cut(s) 470
BbsI GAAGAC 2 cut(s) 300, 306
BbvI GCAGC 4 cut(s) 331, 536, 1273, 1310
BccI CCATC 5 cut(s) 354, 497, 590, 1115, 1169
BciT130I CCWGG 1 cut(s) 1327
BcoDI GTCTC 3 cut(s) 380, 1261, 1382
BfaI CTAG 1 cut(s) 810
BisI GCNGC 5 cut(s) 345, 522, 525, 1287, 1299
BlsI GCNGC 5 cut(s) 346, 523, 526, 1288, 1300
Bme1390I CCNGG 1 cut(s) 1327
BmiI GGNNCC 3 cut(s) 133, 238, 1324
BmrFI CCNGG 1 cut(s) 1327
BmsI GCATC 3 cut(s) 733, 947, 1204
BoxI GACNNNNGTC 1 cut(s) 830
BpiI GAAGAC 2 cut(s) 300, 306
BpmI CTGGAG 3 cut(s) 423, 549, 1230
Bpu10I CCTNAGC 1 cut(s) 1331
Bpu14I TTCGAA 1 cut(s) 1396
BpuEI CTTGAG 2 cut(s) 202, 1267
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 195, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 1332
Bse118I RCCGGY 2 cut(s) 239, 591
Bse1I ACTGG 1 cut(s) 1190
Bse3DI GCAATG 2 cut(s) 504, 615
BseBI CCWGG 1 cut(s) 1327
BseDI CCNNGG 2 cut(s) 195, 387
BseGI GGATG 6 cut(s) 259, 268, 370, 418, 508, 776
BseLI CCNNNNNNNGG 2 cut(s) 388, 1332
BseMI GCAATG 2 cut(s) 504, 615
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 1190
BseRI GAGGAG 4 cut(s) 5, 79, 82, 488
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 4 cut(s) 331, 536, 1273, 1310
Bsh1236I CGCG 2 cut(s) 152, 1346
BshFI GGCC 3 cut(s) 381, 595, 1160
BsiSI CCGG 2 cut(s) 240, 592
BslFI GGGAC 3 cut(s) 667, 995, 1162
BslI CCNNNNNNNGG 2 cut(s) 388, 1332
BsmAI GTCTC 3 cut(s) 380, 1261, 1382
BsmBI CGTCTC 1 cut(s) 1382
BsmFI GGGAC 3 cut(s) 667, 995, 1162
BsnI GGCC 3 cut(s) 381, 595, 1160
Bso31I GGTCTC 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 1396
Bsp1286I GDGCHC 1 cut(s) 922
Bsp143I GATC 2 cut(s) 1230, 1361
Bsp19I CCATGG 2 cut(s) 195, 387
BspACI CCGC 9 cut(s) 60, 69, 150, 200, 234, 521, 1001, 1053, 1346
BspANI GGCC 3 cut(s) 381, 595, 1160
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 2 cut(s) 152, 1346
BspLI GGNNCC 3 cut(s) 133, 238, 1324
BspPI GGATC 1 cut(s) 1238
BspT104I TTCGAA 1 cut(s) 1396
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 504, 615
BsrFI RCCGGY 2 cut(s) 239, 591
BsrI ACTGG 1 cut(s) 1190
BssAI RCCGGY 2 cut(s) 239, 591
BssECI CCNNGG 2 cut(s) 195, 387
BssMI GATC 2 cut(s) 1230, 1361
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 2 cut(s) 195, 387
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 1327
Bst4CI ACNGT 3 cut(s) 721, 1241, 1306
BstAPI GCANNNNNTGC 2 cut(s) 926, 1295
BstBI TTCGAA 1 cut(s) 1396
BstC8I GCNNGC 5 cut(s) 616, 762, 895, 1187, 1356
BstDEI CTNAG 2 cut(s) 354, 1331
BstDSI CCRYGG 2 cut(s) 195, 387
BstEII GGTNACC 1 cut(s) 629
BstF5I GGATG 6 cut(s) 259, 268, 370, 418, 508, 776
BstFNI CGCG 2 cut(s) 152, 1346
BstKTI GATC 2 cut(s) 1233, 1364
BstMAI GTCTC 3 cut(s) 380, 1261, 1382
BstMBI GATC 2 cut(s) 1230, 1361
BstMWI GCNNNNNNNGC 5 cut(s) 68, 926, 935, 1214, 1295
BstNI CCWGG 1 cut(s) 1327
BstNSI RCATGY 2 cut(s) 1203, 1370
BstPAI GACNNNNGTC 1 cut(s) 830
BstPI GGTNACC 1 cut(s) 629
BstSCI CCNGG 1 cut(s) 1325
BstSLI GKGCMC 1 cut(s) 922
BstUI CGCG 2 cut(s) 152, 1346
BstV1I GCAGC 4 cut(s) 331, 536, 1273, 1310
BstV2I GAAGAC 2 cut(s) 300, 306
BstX2I RGATCY 1 cut(s) 1230
BstXI CCANNNNNNTGG 1 cut(s) 284
BstYI RGATCY 1 cut(s) 1230
BsuRI GGCC 3 cut(s) 381, 595, 1160
BtgI CCRYGG 2 cut(s) 195, 387
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 259, 268, 370, 418, 508, 776
BtsIMutI CAGTG 1 cut(s) 726
Cac8I GCNNGC 5 cut(s) 616, 762, 895, 1187, 1356
CaiI CAGNNNCTG 1 cut(s) 1304
Cfr10I RCCGGY 2 cut(s) 239, 591
CseI GACGC 1 cut(s) 1352
Csp6I GTAC 1 cut(s) 753
CspCI CAANNNNNGTGG 2 cut(s) 1290, 1325
CviAII CATG 7 cut(s) 196, 326, 388, 1087, 1182, 1200, 1367
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 2 cut(s) 354, 1331
DpnI GATC 2 cut(s) 1232, 1363
DpnII GATC 2 cut(s) 1230, 1361
EaeI YGGCCR 1 cut(s) 379
EciI GGCGGA 3 cut(s) 49, 215, 249
Eco130I CCWWGG 2 cut(s) 195, 387
Eco31I GGTCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 1209
Eco91I GGTNACC 1 cut(s) 629
EcoO65I GGTNACC 1 cut(s) 629
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 1325
EcoT14I CCWWGG 2 cut(s) 195, 387
EcoT22I ATGCAT 1 cut(s) 1219
ErhI CCWWGG 2 cut(s) 195, 387
Esp3I CGTCTC 1 cut(s) 1382
FaeI CATG 7 cut(s) 199, 329, 391, 1090, 1185, 1203, 1370
FaqI GGGAC 3 cut(s) 667, 995, 1162
FatI CATG 7 cut(s) 195, 325, 387, 1086, 1181, 1199, 1366
FauNDI CATATG 1 cut(s) 1219
Fnu4HI GCNGC 5 cut(s) 345, 522, 525, 1287, 1299
FokI GGATG 6 cut(s) 266, 275, 377, 425, 515, 763
Fsp4HI GCNGC 5 cut(s) 345, 522, 525, 1287, 1299
FspBI CTAG 1 cut(s) 810
GluI GCNGC 5 cut(s) 345, 522, 525, 1287, 1299
GsuI CTGGAG 3 cut(s) 423, 549, 1230
HaeIII GGCC 3 cut(s) 381, 595, 1160
HapII CCGG 2 cut(s) 240, 592
HgaI GACGC 1 cut(s) 1352
Hin1II CATG 7 cut(s) 199, 329, 391, 1090, 1185, 1203, 1370
HincII GTYRAC 1 cut(s) 1316
HindII GTYRAC 1 cut(s) 1316
HinfI GANTC 2 cut(s) 797, 1125
HpaII CCGG 2 cut(s) 240, 592
HphI GGTGA 3 cut(s) 113, 281, 641
Hpy166II GTNNAC 1 cut(s) 1316
Hpy188I TCNGA 3 cut(s) 167, 943, 1261
Hpy188III TCNNGA 8 cut(s) 277, 402, 470, 740, 866, 1246, 1340, 1413
Hpy8I GTNNAC 1 cut(s) 1316
Hpy99I CGWCG 2 cut(s) 308, 669
HpyAV CCTTC 3 cut(s) 260, 839, 1381
HpyCH4III ACNGT 3 cut(s) 721, 1241, 1306
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 7 cut(s) 746, 764, 954, 1016, 1217, 1289, 1298
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 926, 935, 1214, 1295
HpyF3I CTNAG 2 cut(s) 354, 1331
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 7 cut(s) 199, 329, 391, 1090, 1185, 1203, 1370
Kzo9I GATC 2 cut(s) 1230, 1361
LmnI GCTCC 3 cut(s) 167, 236, 697
Lsp1109I GCAGC 4 cut(s) 331, 536, 1273, 1310
LweI GCATC 3 cut(s) 733, 947, 1204
MaeI CTAG 1 cut(s) 810
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 5 cut(s) 587, 629, 931, 1043, 1114
MalI GATC 2 cut(s) 1232, 1363
MboI GATC 2 cut(s) 1230, 1361
MboII GAAGA 3 cut(s) 305, 311, 911
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 1 cut(s) 1230
MhlI GDGCHC 1 cut(s) 922
MluCI AATT 9 cut(s) 24, 212, 248, 311, 334, 485, 679, 790, 1068
MmeI TCCRAC 1 cut(s) 567
Mph1103I ATGCAT 1 cut(s) 1219
MseI TTAA 2 cut(s) 465, 789
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 2 cut(s) 240, 592
MspR9I CCNGG 1 cut(s) 1327
MunI CAATTG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 1327
MvnI CGCG 2 cut(s) 152, 1346
MwoI GCNNNNNNNGC 5 cut(s) 68, 926, 935, 1214, 1295
NcoI CCATGG 2 cut(s) 195, 387
NdeI CATATG 1 cut(s) 1219
NdeII GATC 2 cut(s) 1230, 1361
NlaIII CATG 7 cut(s) 199, 329, 391, 1090, 1185, 1203, 1370
NlaIV GGNNCC 3 cut(s) 133, 238, 1324
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 4 cut(s) 587, 629, 931, 1043
NsiI ATGCAT 1 cut(s) 1219
NspI RCATGY 2 cut(s) 1203, 1370
NspV TTCGAA 1 cut(s) 1396
PciI ACATGT 1 cut(s) 1199
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 2 cut(s) 797, 1125
PflMI CCANNNNNTGG 1 cut(s) 388
PkrI GCNGC 5 cut(s) 346, 523, 526, 1288, 1300
PscI ACATGT 1 cut(s) 1199
PshAI GACNNNNGTC 1 cut(s) 830
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 1325
PspEI GGTNACC 1 cut(s) 629
PspGI CCWGG 1 cut(s) 1325
PspN4I GGNNCC 3 cut(s) 133, 238, 1324
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 1 cut(s) 1230
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
RseI CAYNNNNRTG 1 cut(s) 969
SaqAI TTAA 2 cut(s) 465, 789
SatI GCNGC 5 cut(s) 345, 522, 525, 1287, 1299
Sau3AI GATC 2 cut(s) 1230, 1361
ScrFI CCNGG 1 cut(s) 1327
SduI GDGCHC 1 cut(s) 922
SfaNI GCATC 3 cut(s) 733, 947, 1204
SfuI TTCGAA 1 cut(s) 1396
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 2 cut(s) 181, 1246
SmoI CTYRAG 2 cut(s) 181, 1246
Sse9I AATT 9 cut(s) 24, 212, 248, 311, 334, 485, 679, 790, 1068
SsiI CCGC 9 cut(s) 60, 69, 150, 200, 234, 521, 1001, 1053, 1346
SspMI CTAG 1 cut(s) 810
StyD4I CCNGG 1 cut(s) 1325
StyI CCWWGG 2 cut(s) 195, 387
TaaI ACNGT 3 cut(s) 721, 1241, 1306
TaiI ACGT 1 cut(s) 754
TaqI TCGA 2 cut(s) 1360, 1396
TasI AATT 9 cut(s) 24, 212, 248, 311, 334, 485, 679, 790, 1068
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 797, 1125
Tru1I TTAA 2 cut(s) 465, 789
Tru9I TTAA 2 cut(s) 465, 789
TscAI CASTG 1 cut(s) 726
TseFI GTSAC 4 cut(s) 587, 629, 931, 1043
TseI GCWGC 4 cut(s) 344, 524, 1286, 1298
Tsp45I GTSAC 4 cut(s) 587, 629, 931, 1043
TspDTI ATGAA 5 cut(s) 279, 304, 306, 324, 1041
TspGWI ACGGA 4 cut(s) 720, 1092, 1164, 1364
TspRI CASTG 1 cut(s) 726
Van91I CCANNNNNTGG 1 cut(s) 388
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 2 cut(s) 311, 679
XceI RCATGY 2 cut(s) 1203, 1370
XspI CTAG 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.