Rh1AG473800

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015300
Physical Location & Seq
Forward (+)
68500610 .. 68503160
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG473800.1

Sequence Viewer

Length: 1197 bp
ATGACGACCAGAGGTGCAGATTTTGAGACTAATGCTGATGACGATGACGATGACGATGAAGAAGAGTACGCATACATTGAAGATGAAGAAGAATTAGATGCCGACCCTTTTTTGCCTGAGAGATGGGATGTTGTGGGTCTTGGACAAGCCATGGTGGACTTTTCCGGCATGGTTGATGATGAGTTTCTAGGAAAGCTGGATTTAGAAAAGGGAACAAGGAAGCTTGTGAATCACGAAGAGAGAGGTAGAGTCTTGAGAGCAATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGGTCCCTTTCCAACAGTCTAGTGGCCTTGGCCAGGCTTGGCAGTACATCCATTGGAGGCTCTCCCTTAAGTGTAGCTATGACAGGGAGTGTAGGGAGTGATCCGTTGGGTGGCTTCTACAGGGCAAAGTTACGACGAGCAAATTTGCATTTTCTTTCTCCACCTATCAAAGATGGGACAACAGGGACAGTAATAGTTCTCACTTCTTCAGATGCCCACCGTACAATGCTGGCATATCAGGGAACATCTTCGACTGTTAATTATGATTCATGCTTGGCTAGTGCAGTTTGCAATACAAAAATACTTGTAGTTGAGGGGTATTTGTTTGAACTTCCTGATACAATCAAAACAATTACAAAAGCCTGTGAAGAAGCACGCAGGTGTGGAGCGCTGGTCGCAGTTACAGCATCAGATGTTTCCTGCATTGAGAGACACTATGATGATTTCTGGGAAATTGTTGGGAACTATGCAGACATTGTTTTTGCAAACAGTGATGAAGCAAGAGCCTTCTGTCATTTTTCTTCCAAGGAGAGCCCCATTTCGGCTACAAGGTATCTCAGCCATTTTGTTCCATTCGCCTCGGTTACTGATGGACCAAGAGGCTCTTACATCGGTGTAAAAGGAGAAGCTGTATATATTCCTCCTTCTCCTTGTGTACCAGTGGACACTTGTGGTGCCGGGGATGCATATGCATCAGGAATTTTGTTTGGTATTTTACGAGGAGTGTCAGATTTAAAAGGAATAGGTACATTAGCAGCAAGGGTTGCATCCACGGTTGTTGGACAACAAGGCACTCGGCTTAGGGTTCAAGATGCAGTAGAGTTGGCAGAATCATTCACATTCCATCTCGATAGTTCCACTATTAGATCAGATATCGGGTCAGATCATATTTCCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

398

Amino Acids

42.62

Weight (kDa)

4.61

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 93 - 365 7.6e-34 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 663
AccB1I GGYRCC 1 cut(s) 968
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 2 cut(s) 436, 993
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 5 cut(s) 68, 340, 517, 951, 1042
AfeI AGCGCT 1 cut(s) 684
AfiI CCNNNNNNNGG 3 cut(s) 327, 404, 835
AflII CTTAAG 1 cut(s) 361
AgsI TTSAA 3 cut(s) 80, 623, 1103
AjnI CCWGG 1 cut(s) 326
AleI CACNNNNGTG 1 cut(s) 673
AluBI AGCT 6 cut(s) 196, 223, 276, 371, 923, 1191
AluI AGCT 6 cut(s) 196, 223, 276, 371, 923, 1191
Alw26I GTCTC 2 cut(s) 20, 718
AlwI GGATC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 684
AoxI GGCC 2 cut(s) 318, 324
ApeKI GCWGC 3 cut(s) 284, 287, 1049
ApoI RAATTY 2 cut(s) 436, 993
ArsI GACNNNNNNTTYG 2 cut(s) 758, 790
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 297, 887
AsuC2I CCSGG 1 cut(s) 973
AvaII GGWCC 2 cut(s) 297, 887
BalI TGGCCA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 1 cut(s) 830
BbvI GCAGC 3 cut(s) 271, 274, 1061
BccI CCATC 5 cut(s) 117, 260, 461, 878, 1146
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 1 cut(s) 973
BcoDI GTCTC 2 cut(s) 20, 718
BfaI CTAG 3 cut(s) 188, 314, 573
BfmI CTRYAG 1 cut(s) 412
BfoI RGCGCY 1 cut(s) 686
BfrI CTTAAG 1 cut(s) 361
BfuAI ACCTGC 1 cut(s) 663
BisI GCNGC 3 cut(s) 285, 288, 1050
BlsI GCNGC 3 cut(s) 286, 289, 1051
Bme1390I CCNGG 2 cut(s) 328, 973
Bme18I GGWCC 2 cut(s) 297, 887
BmgT120I GGNCC 2 cut(s) 297, 887
BmiI GGNNCC 3 cut(s) 298, 299, 970
BmrFI CCNGG 2 cut(s) 328, 973
BmsI GCATC 7 cut(s) 88, 496, 710, 967, 995, 1070, 1096
Bpu10I CCTNAGC 1 cut(s) 1094
BpuEI CTTGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 973
BsaJI CCNNGG 6 cut(s) 150, 321, 819, 873, 972, 1065
BsaXI ACNNNNNCTCC 4 cut(s) 672, 702, 909, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 327, 404, 835
Bse1I ACTGG 1 cut(s) 953
Bse3DI GCAATG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 6 cut(s) 150, 321, 819, 873, 972, 1065
BseGI GGATG 5 cut(s) 133, 271, 341, 982, 1061
BseLI CCNNNNNNNGG 3 cut(s) 327, 404, 835
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 2 cut(s) 108, 865
BseNI ACTGG 1 cut(s) 953
BseRI GAGGAG 1 cut(s) 1029
BseXI GCAGC 3 cut(s) 271, 274, 1061
BsgI GTGCAG 2 cut(s) 36, 597
BshFI GGCC 2 cut(s) 320, 326
BshNI GGYRCC 1 cut(s) 968
BsiSI CCGG 2 cut(s) 165, 972
BslFI GGGAC 3 cut(s) 283, 484, 493
BslI CCNNNNNNNGG 3 cut(s) 327, 404, 835
BsmAI GTCTC 2 cut(s) 20, 718
BsmFI GGGAC 3 cut(s) 283, 484, 493
BsnI GGCC 2 cut(s) 320, 326
Bsp1286I GDGCHC 1 cut(s) 830
Bsp143I GATC 3 cut(s) 394, 1160, 1177
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 2 cut(s) 320, 326
BspCNI CTCAG 2 cut(s) 109, 864
BspLI GGNNCC 3 cut(s) 298, 299, 970
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 968
BspTI CTTAAG 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 267
BsrI ACTGG 1 cut(s) 953
BssECI CCNNGG 6 cut(s) 150, 321, 819, 873, 972, 1065
BssMI GATC 3 cut(s) 394, 1160, 1177
BssT1I CCWWGG 3 cut(s) 150, 321, 819
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 6 cut(s) 311, 484, 515, 550, 785, 1069
Bst6I CTCTTC 2 cut(s) 57, 231
BstAFI CTTAAG 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 525, 670
BstDEI CTNAG 3 cut(s) 117, 851, 1094
BstDSI CCRYGG 2 cut(s) 150, 1065
BstENI CCTNNNNNAGG 1 cut(s) 325
BstF5I GGATG 5 cut(s) 133, 271, 341, 982, 1061
BstH2I RGCGCY 1 cut(s) 686
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 3 cut(s) 397, 1163, 1180
BstMAI GTCTC 2 cut(s) 20, 718
BstMBI GATC 3 cut(s) 394, 1160, 1177
BstMWI GCNNNNNNNGC 4 cut(s) 689, 698, 977, 1058
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 326, 971
BstSFI CTRYAG 1 cut(s) 412
BstV1I GCAGC 3 cut(s) 271, 274, 1061
BsuRI GGCC 2 cut(s) 320, 326
BtgI CCRYGG 2 cut(s) 150, 1065
BtsCI GGATG 5 cut(s) 133, 271, 341, 982, 1061
BtsIMutI CAGTG 2 cut(s) 790, 960
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 525, 670
CfoI GCGC 1 cut(s) 685
Cfr13I GGNCC 2 cut(s) 297, 887
Csp6I GTAC 5 cut(s) 67, 339, 516, 950, 1041
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 3 cut(s) 151, 169, 564
CviQI GTAC 5 cut(s) 67, 339, 516, 950, 1041
DdeI CTNAG 3 cut(s) 117, 851, 1094
DpnI GATC 3 cut(s) 396, 1162, 1179
DpnII GATC 3 cut(s) 394, 1160, 1177
DraI TTTAAA 1 cut(s) 1029
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 2 cut(s) 57, 231
EarI CTCTTC 2 cut(s) 57, 231
Eco130I CCWWGG 3 cut(s) 150, 321, 819
Eco24I GRGCYC 1 cut(s) 830
Eco32I GATATC 1 cut(s) 1168
Eco47I GGWCC 2 cut(s) 297, 887
Eco47III AGCGCT 1 cut(s) 684
Eco57I CTGAAG 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 326
EcoRV GATATC 1 cut(s) 1168
EcoT14I CCWWGG 3 cut(s) 150, 321, 819
EcoT22I ATGCAT 2 cut(s) 982, 988
EcoT38I GRGCYC 1 cut(s) 830
ErhI CCWWGG 3 cut(s) 150, 321, 819
FaeI CATG 3 cut(s) 154, 172, 567
FalI AAGNNNNNCTT 2 cut(s) 883, 915
FaqI GGGAC 3 cut(s) 283, 484, 493
FatI CATG 3 cut(s) 150, 168, 563
FauNDI CATATG 1 cut(s) 982
Fnu4HI GCNGC 3 cut(s) 285, 288, 1050
FokI GGATG 5 cut(s) 140, 278, 328, 989, 1048
FriOI GRGCYC 1 cut(s) 830
Fsp4HI GCNGC 3 cut(s) 285, 288, 1050
FspBI CTAG 3 cut(s) 188, 314, 573
GlaI GCGC 1 cut(s) 684
GluI GCNGC 3 cut(s) 285, 288, 1050
HaeII RGCGCY 1 cut(s) 686
HaeIII GGCC 2 cut(s) 320, 326
HapII CCGG 2 cut(s) 165, 972
HhaI GCGC 1 cut(s) 685
Hin1II CATG 3 cut(s) 154, 172, 567
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 4 cut(s) 229, 249, 560, 1124
HpaII CCGG 2 cut(s) 165, 972
Hpy166II GTNNAC 3 cut(s) 157, 950, 958
Hpy188I TCNGA 5 cut(s) 505, 706, 1024, 1165, 1177
Hpy188III TCNNGA 6 cut(s) 233, 253, 629, 990, 1103, 1142
Hpy8I GTNNAC 3 cut(s) 157, 950, 958
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 2 cut(s) 811, 948
HpyCH4III ACNGT 6 cut(s) 311, 484, 515, 550, 785, 1069
HpyF10VI GCNNNNNNNGC 4 cut(s) 689, 698, 977, 1058
HpyF3I CTNAG 3 cut(s) 117, 851, 1094
Hsp92II CATG 3 cut(s) 154, 172, 567
HspAI GCGC 1 cut(s) 683
KflI GGGWCCC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 394, 1160, 1177
LmnI GCTCC 1 cut(s) 680
Lsp1109I GCAGC 3 cut(s) 271, 274, 1061
LweI GCATC 7 cut(s) 88, 496, 710, 967, 995, 1070, 1096
MaeI CTAG 3 cut(s) 188, 314, 573
MaeIII GTNAC 3 cut(s) 423, 694, 877
MalI GATC 3 cut(s) 396, 1162, 1179
MboI GATC 3 cut(s) 394, 1160, 1177
MhlI GDGCHC 1 cut(s) 830
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 6 cut(s) 92, 436, 553, 645, 747, 993
MluNI TGGCCA 1 cut(s) 326
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 2 cut(s) 330, 1054
MnlI CCTC 8 cut(s) 5, 236, 344, 601, 883, 887, 945, 1007
Mox20I TGGCCA 1 cut(s) 326
Mph1103I ATGCAT 2 cut(s) 982, 988
MroXI GAANNNNTTC 1 cut(s) 541
MscI TGGCCA 1 cut(s) 326
MseI TTAA 3 cut(s) 362, 552, 1028
MslI CAYNNNNRTG 2 cut(s) 673, 732
Msp20I TGGCCA 1 cut(s) 326
MspCI CTTAAG 1 cut(s) 361
MspI CCGG 2 cut(s) 165, 972
MspR9I CCNGG 2 cut(s) 328, 973
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 4 cut(s) 689, 698, 977, 1058
NciI CCSGG 1 cut(s) 973
NcoI CCATGG 1 cut(s) 150
NdeI CATATG 1 cut(s) 982
NdeII GATC 3 cut(s) 394, 1160, 1177
NlaIII CATG 3 cut(s) 154, 172, 567
NlaIV GGNNCC 3 cut(s) 298, 299, 970
NmeAIII GCCGAG 1 cut(s) 1069
NsiI ATGCAT 2 cut(s) 982, 988
OliI CACNNNNGTG 1 cut(s) 673
PaqCI CACCTGC 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 3 cut(s) 229, 560, 1124
PkrI GCNGC 3 cut(s) 286, 289, 1051
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 3 cut(s) 298, 299, 970
PspPI GGNCC 2 cut(s) 297, 887
PspPPI RGGWCCY 1 cut(s) 297
RsaI GTAC 5 cut(s) 68, 340, 517, 951, 1042
RsaNI GTAC 5 cut(s) 67, 339, 516, 950, 1041
RseI CAYNNNNRTG 2 cut(s) 673, 732
SaqAI TTAA 3 cut(s) 362, 552, 1028
SatI GCNGC 3 cut(s) 285, 288, 1050
Sau3AI GATC 3 cut(s) 394, 1160, 1177
Sau96I GGNCC 2 cut(s) 297, 887
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 328, 973
SduI GDGCHC 1 cut(s) 830
SfaNI GCATC 7 cut(s) 88, 496, 710, 967, 995, 1070, 1096
SfcI CTRYAG 1 cut(s) 412
SinI GGWCC 2 cut(s) 297, 887
SmiMI CAYNNNNRTG 2 cut(s) 673, 732
SmlI CTYRAG 2 cut(s) 253, 361
SmoI CTYRAG 2 cut(s) 253, 361
Sse9I AATT 6 cut(s) 92, 436, 553, 645, 747, 993
SspMI CTAG 3 cut(s) 188, 314, 573
StyD4I CCNGG 2 cut(s) 326, 971
StyI CCWWGG 3 cut(s) 150, 321, 819
TaaI ACNGT 6 cut(s) 311, 484, 515, 550, 785, 1069
TaqI TCGA 2 cut(s) 545, 1143
TasI AATT 6 cut(s) 92, 436, 553, 645, 747, 993
TatI WGTACW 1 cut(s) 338
TfiI GAWTC 3 cut(s) 229, 560, 1124
Tru1I TTAA 3 cut(s) 362, 552, 1028
Tru9I TTAA 3 cut(s) 362, 552, 1028
TscAI CASTG 2 cut(s) 790, 960
TseI GCWGC 3 cut(s) 284, 287, 1049
TspDTI ATGAA 4 cut(s) 72, 99, 552, 804
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 2 cut(s) 790, 960
Vha464I CTTAAG 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 297, 887
XagI CCTNNNNNAGG 1 cut(s) 325
XapI RAATTY 2 cut(s) 436, 993
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 3 cut(s) 188, 314, 573
Zsp2I ATGCAT 2 cut(s) 982, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.