FvH4_7g34180

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015300
Physical Location & Seq
Forward (+)
24126730 .. 24129180
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g34180.t1

Sequence Viewer

Length: 1347 bp
ATGTCTCTCTCCTCTAGCTTTCCTCCGCCCCACATAATCGGGGGAAGGTGCTCTCCACCAGTATATTCTCCTGGTTTTACTCTGCCGTCCTTGTTTTTAAACCCCGCAGGCACCTCTTCTCATCTCCGTCTCAAACACGCCTCAAGCATTGTTCCTTCTATGACGAGACGTCCTGATTTTCACACCAATGGGAGTGCTGCCGCTGATGATGACGATGAAGATGACGACATTCAATATGAAGAATTAGATGCCGACCCTTTTCTGCCTGAGAGATGGGATGTTTTGGGTCTTGGCCAAGCCATGGTGGACTTTTCGGGCATGGTTGATGATGAGTTTCTGGAAAAACTGGGTTTAGAAAAAGGAACAAGGAAGCTTGTCAATCATGAAGAGAGAGGTAGAGTCTTGAGAGCGATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGCTCCCTTTCCAACAGTCTTGTGGCCTTGGCTAGGCTTGGCAGTACATCCATTGGAGGCTCTCCCTTAAGTGTAGCTATGACAGGGAGTGTAGGGAGTGATCCGTTGGGTGGCTTCTACAGAGCCAAGTTACGTCGGGCAAATCTGCATTTTCTTTCTCCGCCAATCAAAGATGGAACAACAGGGACAGTAATAGTCCTCACTTCCTCAGATGCCCACCGTACAATGCTGGCGTATCAGGGAACATCTTCGACTGTTAACTATGATTCATGCTTGGCTAGTGCAGTTTGCAATACAAAAATACTTGTAGTTGAGGGGTATTTGTTTGAACTTCCTGATACTATCAAAACAATTACAAAAGCCTGTGAAGAAGCACACAGGTGTGGAGCACTGGTTGCAGTTACAGCATCAGATGTTTCCTGCATTGAGAGACACTATGACGATTTCTGGGAAATTGTTGGGAACTATGCAGACATTGTTTTTGCAAACAGTGACGAAGCAAGAGCCTTCTGTCATTTTTCCTCTAAGGAGAGCCCCATTTCGGCTACAAGGTATCTCAGCCATTTTGTTCCATTCGCCTCGGTTACTGATGGACCGAGAGGCTCTTACATTGGTGTAAAAGGAGAAGCTGTATATATCCCTCCTTCTCCATGTGTACCAGTGGACACTTGTGGTGCCGGGGATGCATATGCATCGGGAATTTTGTTTGGCATTTTACGAGGAGTGTCAGATTTAAAAGGAATAGGTACATTAGCAGCTAGGGTTGCATCCACAGTGGTTGGACAGCAAGGCACTCGGCTTAGGGTTCAAGATGCGGTAGAGTTGGCAGAATCATTCACATTTCATCTTGATAGTTCCACTATTAGGTCAGATATTGGGTCAGATCATATATCCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

449

Amino Acids

47.72

Weight (kDa)

5.23

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 143 - 415 9.3e-34 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 172
AccB1I GGYRCC 2 cut(s) 110, 1118
AccB7I CCANNNNNTGG 1 cut(s) 301
AciI CCGC 5 cut(s) 26, 105, 201, 605, 1259
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 1 cut(s) 1143
AcyI GRCGYC 1 cut(s) 169
AfaI GTAC 4 cut(s) 490, 667, 1101, 1192
AfiI CCNNNNNNNGG 5 cut(s) 301, 477, 554, 985, 1308
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 3 cut(s) 233, 773, 1253
AhdI GACNNNNNGTC 1 cut(s) 168
AjnI CCWGG 1 cut(s) 70
AleI CACNNNNGTG 2 cut(s) 823, 825
AluBI AGCT 7 cut(s) 18, 373, 426, 521, 1073, 1202, 1341
AluI AGCT 7 cut(s) 18, 373, 426, 521, 1073, 1202, 1341
Alw21I GWGCWC 2 cut(s) 53, 835
Alw26I GTCTC 4 cut(s) 9, 134, 160, 868
AlwI GGATC 1 cut(s) 539
AoxI GGCC 2 cut(s) 292, 468
ApeKI GCWGC 4 cut(s) 197, 434, 437, 1199
ApoI RAATTY 1 cut(s) 1143
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
Asp700I GAANNNNTTC 1 cut(s) 691
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 1 cut(s) 1123
AvaII GGWCC 1 cut(s) 1037
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 2 cut(s) 110, 1118
BanII GRGCYC 1 cut(s) 980
Bbv12I GWGCWC 2 cut(s) 53, 835
BbvI GCAGC 4 cut(s) 184, 421, 424, 1211
BccI CCATC 5 cut(s) 267, 406, 410, 611, 1028
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 1119, 1153, 1220, 1254
BciT130I CCWGG 1 cut(s) 72
BcnI CCSGG 1 cut(s) 1123
BcoDI GTCTC 4 cut(s) 9, 134, 160, 868
BfaI CTAG 4 cut(s) 15, 477, 723, 1203
BfmI CTRYAG 1 cut(s) 562
BfrI CTTAAG 1 cut(s) 511
BisI GCNGC 5 cut(s) 198, 201, 435, 438, 1200
BlsI GCNGC 5 cut(s) 199, 202, 436, 439, 1201
Bme1390I CCNGG 2 cut(s) 72, 1123
Bme18I GGWCC 1 cut(s) 1037
BmeRI GACNNNNNGTC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 3 cut(s) 112, 448, 1120
BmrFI CCNGG 2 cut(s) 72, 1123
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 7 cut(s) 238, 646, 860, 1117, 1145, 1220, 1246
BmuI ACTGGG 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 1244
BpuEI CTTGAG 2 cut(s) 127, 424
BpuMI CCSGG 1 cut(s) 1123
BsaHI GRCGYC 1 cut(s) 169
BsaJI CCNNGG 4 cut(s) 300, 471, 1023, 1122
BsaXI ACNNNNNCTCC 4 cut(s) 822, 852, 1059, 1089
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 477, 554, 985, 1308
Bse1I ACTGG 4 cut(s) 59, 351, 840, 1103
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 4 cut(s) 300, 471, 1023, 1122
BseGI GGATG 5 cut(s) 283, 421, 491, 1132, 1211
BseLI CCNNNNNNNGG 5 cut(s) 301, 477, 554, 985, 1308
BseMII CTCAG 3 cut(s) 258, 666, 1015
BseNI ACTGG 4 cut(s) 59, 351, 840, 1103
BseRI GAGGAG 1 cut(s) 1179
BseXI GCAGC 4 cut(s) 184, 421, 424, 1211
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 2 cut(s) 294, 470
BshNI GGYRCC 2 cut(s) 110, 1118
BsiHKAI GWGCWC 2 cut(s) 53, 835
BsiSI CCGG 1 cut(s) 1122
BslFI GGGAC 1 cut(s) 643
BslI CCNNNNNNNGG 5 cut(s) 301, 477, 554, 985, 1308
BsmAI GTCTC 4 cut(s) 9, 134, 160, 868
BsmBI CGTCTC 2 cut(s) 134, 160
BsmFI GGGAC 1 cut(s) 643
BsnI GGCC 2 cut(s) 294, 470
Bsp1286I GDGCHC 3 cut(s) 53, 835, 980
Bsp143I GATC 2 cut(s) 544, 1327
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 5 cut(s) 26, 105, 201, 605, 1259
BspANI GGCC 2 cut(s) 294, 470
BspCNI CTCAG 3 cut(s) 259, 665, 1014
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 112, 448, 1120
BspPI GGATC 1 cut(s) 539
BspT107I GGYRCC 2 cut(s) 110, 1118
BspTI CTTAAG 1 cut(s) 511
BsrI ACTGG 4 cut(s) 59, 351, 840, 1103
BssECI CCNNGG 4 cut(s) 300, 471, 1023, 1122
BssMI GATC 2 cut(s) 544, 1327
BssNI GRCGYC 1 cut(s) 169
BssT1I CCWWGG 2 cut(s) 300, 471
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 6 cut(s) 461, 634, 665, 700, 935, 1219
Bst6I CTCTTC 2 cut(s) 121, 381
BstACI GRCGYC 1 cut(s) 169
BstAFI CTTAAG 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 839
BstC8I GCNNGC 2 cut(s) 109, 675
BstDEI CTNAG 5 cut(s) 267, 652, 969, 1001, 1244
BstDSI CCRYGG 1 cut(s) 300
BstENI CCTNNNNNAGG 1 cut(s) 475
BstF5I GGATG 5 cut(s) 283, 421, 491, 1132, 1211
BstKTI GATC 2 cut(s) 547, 1330
BstMAI GTCTC 4 cut(s) 9, 134, 160, 868
BstMBI GATC 2 cut(s) 544, 1327
BstMWI GCNNNNNNNGC 4 cut(s) 839, 848, 1127, 1208
BstNI CCWGG 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 70, 1121
BstSFI CTRYAG 1 cut(s) 562
BstV1I GCAGC 4 cut(s) 184, 421, 424, 1211
BsuRI GGCC 2 cut(s) 294, 470
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 5 cut(s) 283, 421, 491, 1132, 1211
BtsIMutI CAGTG 4 cut(s) 833, 940, 1110, 1224
Cac8I GCNNGC 2 cut(s) 109, 675
CciI TCATGA 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 1037
Csp6I GTAC 4 cut(s) 489, 666, 1100, 1191
CspCI CAANNNNNGTGG 2 cut(s) 1204, 1239
CviAII CATG 5 cut(s) 301, 319, 383, 714, 1095
CviQI GTAC 4 cut(s) 489, 666, 1100, 1191
DdeI CTNAG 5 cut(s) 267, 652, 969, 1001, 1244
DpnI GATC 2 cut(s) 546, 1329
DpnII GATC 2 cut(s) 544, 1327
DraI TTTAAA 2 cut(s) 99, 1179
DriI GACNNNNNGTC 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 121, 381
Eam1105I GACNNNNNGTC 1 cut(s) 168
EarI CTCTTC 2 cut(s) 121, 381
EciI GGCGGA 2 cut(s) 15, 594
Eco130I CCWWGG 2 cut(s) 300, 471
Eco24I GRGCYC 1 cut(s) 980
Eco47I GGWCC 1 cut(s) 1037
EcoNI CCTNNNNNAGG 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 300, 471
EcoT22I ATGCAT 2 cut(s) 1132, 1138
EcoT38I GRGCYC 1 cut(s) 980
ErhI CCWWGG 2 cut(s) 300, 471
Esp3I CGTCTC 2 cut(s) 134, 160
FaeI CATG 5 cut(s) 304, 322, 386, 717, 1098
FaqI GGGAC 1 cut(s) 643
FatI CATG 5 cut(s) 300, 318, 382, 713, 1094
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 1132
Fnu4HI GCNGC 5 cut(s) 198, 201, 435, 438, 1200
FokI GGATG 5 cut(s) 290, 428, 478, 1139, 1198
FriOI GRGCYC 1 cut(s) 980
Fsp4HI GCNGC 5 cut(s) 198, 201, 435, 438, 1200
FspBI CTAG 4 cut(s) 15, 477, 723, 1203
GluI GCNGC 5 cut(s) 198, 201, 435, 438, 1200
HaeIII GGCC 2 cut(s) 294, 470
HapII CCGG 1 cut(s) 1122
Hin1I GRCGYC 1 cut(s) 169
Hin1II CATG 5 cut(s) 304, 322, 386, 717, 1098
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 399, 710, 1274
HpaI GTTAAC 1 cut(s) 703
HpaII CCGG 1 cut(s) 1122
Hpy166II GTNNAC 4 cut(s) 307, 703, 1100, 1108
Hpy188I TCNGA 5 cut(s) 655, 856, 1174, 1315, 1327
Hpy188III TCNNGA 8 cut(s) 173, 338, 383, 403, 779, 1140, 1253, 1292
Hpy8I GTNNAC 4 cut(s) 307, 703, 1100, 1108
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 4 cut(s) 39, 165, 961, 1098
HpyCH4III ACNGT 6 cut(s) 461, 634, 665, 700, 935, 1219
HpyCH4IV ACGT 2 cut(s) 169, 577
HpyF10VI GCNNNNNNNGC 4 cut(s) 839, 848, 1127, 1208
HpyF3I CTNAG 5 cut(s) 267, 652, 969, 1001, 1244
HpySE526I ACGT 2 cut(s) 169, 577
Hsp92I GRCGYC 1 cut(s) 169
Hsp92II CATG 5 cut(s) 304, 322, 386, 717, 1098
KspAI GTTAAC 1 cut(s) 703
Kzo9I GATC 2 cut(s) 544, 1327
LmnI GCTCC 2 cut(s) 452, 830
Lsp1109I GCAGC 4 cut(s) 184, 421, 424, 1211
LweI GCATC 7 cut(s) 238, 646, 860, 1117, 1145, 1220, 1246
MaeI CTAG 4 cut(s) 15, 477, 723, 1203
MaeII ACGT 2 cut(s) 169, 577
MaeIII GTNAC 4 cut(s) 573, 844, 935, 1027
MalI GATC 2 cut(s) 546, 1329
MboI GATC 2 cut(s) 544, 1327
MboII GAAGA 6 cut(s) 108, 230, 251, 398, 684, 824
MhlI GDGCHC 3 cut(s) 53, 835, 980
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 4 cut(s) 242, 795, 897, 1143
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 1 cut(s) 408
MmeI TCCRAC 2 cut(s) 480, 1204
Mox20I TGGCCA 1 cut(s) 294
Mph1103I ATGCAT 2 cut(s) 1132, 1138
MroXI GAANNNNTTC 1 cut(s) 691
MscI TGGCCA 1 cut(s) 294
MseI TTAA 4 cut(s) 98, 512, 702, 1178
MslI CAYNNNNRTG 3 cut(s) 186, 823, 825
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 203
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 1 cut(s) 1122
MspR9I CCNGG 2 cut(s) 72, 1123
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 4 cut(s) 839, 848, 1127, 1208
NciI CCSGG 1 cut(s) 1123
NcoI CCATGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 1132
NdeII GATC 2 cut(s) 544, 1327
NlaIII CATG 5 cut(s) 304, 322, 386, 717, 1098
NlaIV GGNNCC 3 cut(s) 112, 448, 1120
NmeAIII GCCGAG 1 cut(s) 1219
NmuCI GTSAC 1 cut(s) 935
NsiI ATGCAT 2 cut(s) 1132, 1138
OliI CACNNNNGTG 2 cut(s) 823, 825
PagI TCATGA 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 2 cut(s) 710, 1274
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 5 cut(s) 199, 202, 436, 439, 1201
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 3 cut(s) 112, 448, 1120
PspPI GGNCC 1 cut(s) 1037
RsaI GTAC 4 cut(s) 490, 667, 1101, 1192
RsaNI GTAC 4 cut(s) 489, 666, 1100, 1191
RseI CAYNNNNRTG 3 cut(s) 186, 823, 825
SaqAI TTAA 4 cut(s) 98, 512, 702, 1178
SatI GCNGC 5 cut(s) 198, 201, 435, 438, 1200
Sau3AI GATC 2 cut(s) 544, 1327
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 2 cut(s) 72, 1123
SduI GDGCHC 3 cut(s) 53, 835, 980
SfaNI GCATC 7 cut(s) 238, 646, 860, 1117, 1145, 1220, 1246
SfcI CTRYAG 1 cut(s) 562
SinI GGWCC 1 cut(s) 1037
SmiMI CAYNNNNRTG 3 cut(s) 186, 823, 825
SmlI CTYRAG 3 cut(s) 142, 403, 511
SmoI CTYRAG 3 cut(s) 142, 403, 511
Sse9I AATT 4 cut(s) 242, 795, 897, 1143
SsiI CCGC 5 cut(s) 26, 105, 201, 605, 1259
SspMI CTAG 4 cut(s) 15, 477, 723, 1203
StyD4I CCNGG 2 cut(s) 70, 1121
StyI CCWWGG 2 cut(s) 300, 471
TaaI ACNGT 6 cut(s) 461, 634, 665, 700, 935, 1219
TaiI ACGT 2 cut(s) 172, 580
TaqI TCGA 1 cut(s) 695
TaqII GACCGA 1 cut(s) 1054
TasI AATT 4 cut(s) 242, 795, 897, 1143
TatI WGTACW 1 cut(s) 488
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 2 cut(s) 710, 1274
Tru1I TTAA 4 cut(s) 98, 512, 702, 1178
Tru9I TTAA 4 cut(s) 98, 512, 702, 1178
TscAI CASTG 4 cut(s) 840, 940, 1110, 1224
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 4 cut(s) 197, 434, 437, 1199
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 5 cut(s) 231, 252, 399, 702, 1277
TspGWI ACGGA 2 cut(s) 116, 537
TspRI CASTG 4 cut(s) 840, 940, 1110, 1224
Van91I CCANNNNNTGG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 1037
XagI CCTNNNNNAGG 1 cut(s) 475
XapI RAATTY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 4 cut(s) 15, 477, 723, 1203
ZraI GACGTC 1 cut(s) 170
Zsp2I ATGCAT 2 cut(s) 1132, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.