Rmu_sc0006005.1_g000007

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006005.1
OG0015300
Physical Location & Seq
Forward (+)
23413 .. 27169
3757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006005.1_g000007.1.cds

Sequence Viewer

Length: 1509 bp
atgtctctctcctcttcctttgttccccagataatcggcagcaggtcctctccaccagtctattctcctggttttcctctccagcttcctcgcgtttctctctttttatacccctcatctttgggtccaccacatctccatcacaaagccaccccaaagaatattgttgcttctatggcgaccagaggtgcagattttgagactactgctgatgacgatgaagaagagtacgcatacattgaagatgaagaattagatgccgacccttttttgcctgagagatgggatgttgtgggtcttggacaagccatggtggacttttccggcatggttgatgatgagtttctggaaaaactggatttagaaaagggaacaaggaagcttgtgaatcacgaagagagaggtagagtcttgagagcaatggatggttgtagctataaggctgctgctggtgggtccctttccaacagtctagtggccttggccaggcttggcagtacatccattggaggctctcccttaagtgtagctatgacagggagtgtagggagtgatccgttgggtggcttctacagagcaaagttacgacgggcaaatttgcattttctttctccacctattaaagatgggacaacagggacggtaatagttctcacttcttcagatgcccaccgtacaatgctggcatatcagggaacatcttcaactgttaattatgattcatgcttggctagtgcagtttgcaatacaaaaatacttgtagttgaggggtatttgtttgaacttcctgatacaatcaaaacaattacaaaagcctgtgaagaagcacgcaggtgtggagcgctggtcgcagttacagcatcagatgtttcctgcattgagagacactatgatgatttctgggaaattgttgggaactatgcagacattgtttttgcaaacagtgatgaagcaagagccttctgtcatttttcttccaaggagagccccatttcggctacaaggtatctcagccattttgttccattcgcctcggttactgatggaccaagaggctcttacatcggtgtaaaaggagaagctgtatatattcctccttctccttgtgtaccagtggacacttgtggtgccggggatgcatatgcatcaggaattttgtttggtattttacgaggagtgtcagatttaaaaggaataggttcattagcagcaagggttgcatccacggttgttggacaacaaggcactcggcttagggttcaagatgcagtagagttggcagaatcattcacattccatctcgatagttccactattagatcagatatcggggtgcacaagccaaagaaggaacctggtgttcacaaaggggactctggggcagggctttcagaaccacaccttaatactggagaagctgatgagaaccggagacgctgtcttttgcggcaagacaagtgcccctcatctctggaccctactgcatcccctttgtcgtcaacctcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

502

Amino Acids

53.65

Weight (kDa)

5.37

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 822
Acc36I ACCTGC 2 cut(s) 33, 822
AccB1I GGYRCC 1 cut(s) 1127
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 1447
AclWI GGATC 1 cut(s) 548
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 2 cut(s) 595, 1152
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 4 cut(s) 230, 499, 676, 1110
AfeI AGCGCT 1 cut(s) 843
AfiI CCNNNNNNNGG 3 cut(s) 486, 563, 994
AflII CTTAAG 1 cut(s) 520
AgsI TTSAA 4 cut(s) 242, 705, 782, 1262
AjnI CCWGG 3 cut(s) 67, 485, 1354
AleI CACNNNNGTG 1 cut(s) 832
AluBI AGCT 6 cut(s) 85, 382, 435, 530, 1082, 1418
AluI AGCT 6 cut(s) 85, 382, 435, 530, 1082, 1418
Alw21I GWGCWC 1 cut(s) 1338
Alw26I GTCTC 4 cut(s) 9, 194, 877, 1426
Alw44I GTGCAC 1 cut(s) 1334
AlwI GGATC 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 843
AoxI GGCC 2 cut(s) 477, 483
ApaLI GTGCAC 1 cut(s) 1334
ApeKI GCWGC 4 cut(s) 39, 443, 446, 1208
ApoI RAATTY 2 cut(s) 595, 1152
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
Asp700I GAANNNNTTC 2 cut(s) 700, 1198
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 5 cut(s) 45, 125, 456, 1046, 1474
AsuC2I CCSGG 1 cut(s) 1132
AvaII GGWCC 5 cut(s) 45, 125, 456, 1046, 1474
BaeGI GKGCMC 2 cut(s) 1338, 1463
BalI TGGCCA 1 cut(s) 485
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 1 cut(s) 989
Bbv12I GWGCWC 1 cut(s) 1338
BbvI GCAGC 4 cut(s) 51, 430, 433, 1220
BccI CCATC 6 cut(s) 147, 276, 419, 620, 1037, 1305
BciT130I CCWGG 3 cut(s) 69, 487, 1356
BcnI CCSGG 1 cut(s) 1132
BcoDI GTCTC 4 cut(s) 9, 194, 877, 1426
BfaI CTAG 2 cut(s) 473, 732
BfmI CTRYAG 1 cut(s) 571
BfoI RGCGCY 1 cut(s) 845
BfrI CTTAAG 1 cut(s) 520
BfuAI ACCTGC 2 cut(s) 33, 822
BisI GCNGC 5 cut(s) 40, 444, 447, 1209, 1448
BlsI GCNGC 5 cut(s) 41, 445, 448, 1210, 1449
Bme1390I CCNGG 4 cut(s) 69, 487, 1132, 1356
Bme18I GGWCC 5 cut(s) 45, 125, 456, 1046, 1474
BmgT120I GGNCC 5 cut(s) 45, 125, 456, 1046, 1474
BmiI GGNNCC 6 cut(s) 126, 457, 458, 1129, 1353, 1476
BmrFI CCNGG 4 cut(s) 69, 487, 1132, 1356
BmsI GCATC 8 cut(s) 247, 655, 869, 1126, 1154, 1229, 1255, 1493
BpmI CTGGAG 2 cut(s) 65, 1431
Bpu10I CCTNAGC 1 cut(s) 1253
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 1132
BsaJI CCNNGG 6 cut(s) 309, 480, 978, 1032, 1131, 1224
BsaWI WCCGGW 1 cut(s) 1428
BsaXI ACNNNNNCTCC 8 cut(s) 120, 150, 831, 861, 1068, 1098, 1423, 1453
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 563, 994
Bse1I ACTGG 4 cut(s) 56, 360, 1112, 1414
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 3 cut(s) 69, 487, 1356
BseDI CCNNGG 6 cut(s) 309, 480, 978, 1032, 1131, 1224
BseGI GGATG 6 cut(s) 292, 430, 500, 1141, 1220, 1484
BseLI CCNNNNNNNGG 3 cut(s) 486, 563, 994
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 267, 1024
BseNI ACTGG 4 cut(s) 56, 360, 1112, 1414
BseRI GAGGAG 1 cut(s) 1188
BseSI GKGCMC 2 cut(s) 1338, 1463
BseXI GCAGC 4 cut(s) 51, 430, 433, 1220
BsgI GTGCAG 2 cut(s) 210, 756
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 479, 485
BshNI GGYRCC 1 cut(s) 1127
BsiHKAI GWGCWC 1 cut(s) 1338
BsiSI CCGG 3 cut(s) 324, 1131, 1429
BslFI GGGAC 4 cut(s) 442, 643, 652, 1385
BslI CCNNNNNNNGG 3 cut(s) 486, 563, 994
BsmAI GTCTC 4 cut(s) 9, 194, 877, 1426
BsmBI CGTCTC 1 cut(s) 1426
BsmFI GGGAC 4 cut(s) 442, 643, 652, 1385
BsnI GGCC 2 cut(s) 479, 485
Bsp1286I GDGCHC 3 cut(s) 989, 1338, 1463
Bsp143I GATC 2 cut(s) 553, 1319
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 1 cut(s) 1447
BspANI GGCC 2 cut(s) 479, 485
BspCNI CTCAG 2 cut(s) 268, 1023
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 6 cut(s) 126, 457, 458, 1129, 1353, 1476
BspMI ACCTGC 2 cut(s) 33, 822
BspPI GGATC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 1127
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 426
BsrI ACTGG 4 cut(s) 56, 360, 1112, 1414
BssECI CCNNGG 6 cut(s) 309, 480, 978, 1032, 1131, 1224
BssMI GATC 2 cut(s) 553, 1319
BssT1I CCWWGG 3 cut(s) 309, 480, 978
Bst2UI CCWGG 3 cut(s) 69, 487, 1356
Bst4CI ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
Bst6I CTCTTC 3 cut(s) 19, 219, 390
BstAFI CTTAAG 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstC8I GCNNGC 2 cut(s) 684, 829
BstDEI CTNAG 4 cut(s) 276, 1010, 1253, 1506
BstDSI CCRYGG 2 cut(s) 309, 1224
BstENI CCTNNNNNAGG 1 cut(s) 484
BstF5I GGATG 6 cut(s) 292, 430, 500, 1141, 1220, 1484
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 2 cut(s) 556, 1322
BstMAI GTCTC 4 cut(s) 9, 194, 877, 1426
BstMBI GATC 2 cut(s) 553, 1319
BstMWI GCNNNNNNNGC 5 cut(s) 176, 848, 857, 1136, 1217
BstNI CCWGG 3 cut(s) 69, 487, 1356
BstSCI CCNGG 4 cut(s) 67, 485, 1130, 1354
BstSFI CTRYAG 1 cut(s) 571
BstSLI GKGCMC 2 cut(s) 1338, 1463
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 51, 430, 433, 1220
BsuRI GGCC 2 cut(s) 479, 485
BtgI CCRYGG 2 cut(s) 309, 1224
BtsCI GGATG 6 cut(s) 292, 430, 500, 1141, 1220, 1484
BtsIMutI CAGTG 2 cut(s) 949, 1119
BveI ACCTGC 2 cut(s) 33, 822
Cac8I GCNNGC 2 cut(s) 684, 829
CfoI GCGC 1 cut(s) 844
Cfr13I GGNCC 5 cut(s) 45, 125, 456, 1046, 1474
CseI GACGC 1 cut(s) 1443
CsiI ACCWGGT 1 cut(s) 1354
Csp6I GTAC 4 cut(s) 229, 498, 675, 1109
CspCI CAANNNNNGTGG 2 cut(s) 1213, 1248
CviAII CATG 3 cut(s) 310, 328, 723
CviQI GTAC 4 cut(s) 229, 498, 675, 1109
DdeI CTNAG 4 cut(s) 276, 1010, 1253, 1506
DpnI GATC 2 cut(s) 555, 1321
DpnII GATC 2 cut(s) 553, 1319
DraI TTTAAA 1 cut(s) 1188
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 3 cut(s) 19, 219, 390
EarI CTCTTC 3 cut(s) 19, 219, 390
Eco130I CCWWGG 3 cut(s) 309, 480, 978
Eco24I GRGCYC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 1327
Eco47I GGWCC 5 cut(s) 45, 125, 456, 1046, 1474
Eco47III AGCGCT 1 cut(s) 843
Eco57I CTGAAG 1 cut(s) 645
EcoNI CCTNNNNNAGG 1 cut(s) 484
EcoO109I RGGNCCY 2 cut(s) 45, 456
EcoRII CCWGG 3 cut(s) 67, 485, 1354
EcoRV GATATC 1 cut(s) 1327
EcoT14I CCWWGG 3 cut(s) 309, 480, 978
EcoT22I ATGCAT 2 cut(s) 1141, 1147
EcoT38I GRGCYC 1 cut(s) 989
ErhI CCWWGG 3 cut(s) 309, 480, 978
Esp3I CGTCTC 1 cut(s) 1426
FaeI CATG 3 cut(s) 313, 331, 726
FalI AAGNNNNNCTT 2 cut(s) 1042, 1074
FaqI GGGAC 4 cut(s) 442, 643, 652, 1385
FatI CATG 3 cut(s) 309, 327, 722
FauNDI CATATG 1 cut(s) 1141
Fnu4HI GCNGC 5 cut(s) 40, 444, 447, 1209, 1448
FokI GGATG 6 cut(s) 299, 437, 487, 1148, 1207, 1471
FriOI GRGCYC 1 cut(s) 989
Fsp4HI GCNGC 5 cut(s) 40, 444, 447, 1209, 1448
FspBI CTAG 2 cut(s) 473, 732
GlaI GCGC 1 cut(s) 843
GluI GCNGC 5 cut(s) 40, 444, 447, 1209, 1448
GsuI CTGGAG 2 cut(s) 65, 1431
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 2 cut(s) 479, 485
HapII CCGG 3 cut(s) 324, 1131, 1429
HgaI GACGC 1 cut(s) 1443
HhaI GCGC 1 cut(s) 844
Hin1II CATG 3 cut(s) 313, 331, 726
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HincII GTYRAC 1 cut(s) 1500
HindII GTYRAC 1 cut(s) 1500
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 5 cut(s) 388, 408, 719, 1283, 1373
HpaII CCGG 3 cut(s) 324, 1131, 1429
Hpy166II GTNNAC 7 cut(s) 128, 316, 1109, 1117, 1336, 1363, 1500
Hpy188I TCNGA 5 cut(s) 664, 865, 1183, 1324, 1393
Hpy188III TCNNGA 8 cut(s) 347, 392, 412, 788, 1149, 1262, 1301, 1472
Hpy8I GTNNAC 7 cut(s) 128, 316, 1109, 1117, 1336, 1363, 1500
Hpy99I CGWCG 1 cut(s) 591
HpyAV CCTTC 3 cut(s) 970, 1107, 1342
HpyCH4III ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 848, 857, 1136, 1217
HpyF3I CTNAG 4 cut(s) 276, 1010, 1253, 1506
Hsp92II CATG 3 cut(s) 313, 331, 726
HspAI GCGC 1 cut(s) 842
KflI GGGWCCC 1 cut(s) 456
Kzo9I GATC 2 cut(s) 553, 1319
LmnI GCTCC 1 cut(s) 839
Lsp1109I GCAGC 4 cut(s) 51, 430, 433, 1220
LweI GCATC 8 cut(s) 247, 655, 869, 1126, 1154, 1229, 1255, 1493
MabI ACCWGGT 1 cut(s) 1354
MaeI CTAG 2 cut(s) 473, 732
MaeIII GTNAC 3 cut(s) 582, 853, 1036
MalI GATC 2 cut(s) 555, 1321
MboI GATC 2 cut(s) 553, 1319
MhlI GDGCHC 3 cut(s) 989, 1338, 1463
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 417, 1367
MmeI TCCRAC 2 cut(s) 489, 1213
Mox20I TGGCCA 1 cut(s) 485
Mph1103I ATGCAT 2 cut(s) 1141, 1147
MroXI GAANNNNTTC 2 cut(s) 700, 1198
MscI TGGCCA 1 cut(s) 485
MseI TTAA 5 cut(s) 521, 621, 711, 1187, 1404
MslI CAYNNNNRTG 2 cut(s) 832, 891
Msp20I TGGCCA 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 3 cut(s) 324, 1131, 1429
MspR9I CCNGG 4 cut(s) 69, 487, 1132, 1356
MvaI CCWGG 3 cut(s) 69, 487, 1356
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 5 cut(s) 176, 848, 857, 1136, 1217
NciI CCSGG 1 cut(s) 1132
NcoI CCATGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 1141
NdeII GATC 2 cut(s) 553, 1319
NlaIII CATG 3 cut(s) 313, 331, 726
NlaIV GGNNCC 6 cut(s) 126, 457, 458, 1129, 1353, 1476
NmeAIII GCCGAG 1 cut(s) 1228
NsiI ATGCAT 2 cut(s) 1141, 1147
OliI CACNNNNGTG 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 822
PdmI GAANNNNTTC 2 cut(s) 700, 1198
PfeI GAWTC 3 cut(s) 388, 719, 1283
PflFI GACNNNGTC 1 cut(s) 1437
PkrI GCNGC 5 cut(s) 41, 445, 448, 1210, 1449
PleI GAGTC 2 cut(s) 416, 1367
PpsI GAGTC 2 cut(s) 416, 1367
PpuMI RGGWCCY 2 cut(s) 45, 456
Psp5II RGGWCCY 2 cut(s) 45, 456
Psp6I CCWGG 3 cut(s) 67, 485, 1354
PspGI CCWGG 3 cut(s) 67, 485, 1354
PspN4I GGNNCC 6 cut(s) 126, 457, 458, 1129, 1353, 1476
PspPI GGNCC 5 cut(s) 45, 125, 456, 1046, 1474
PspPPI RGGWCCY 2 cut(s) 45, 456
PsyI GACNNNGTC 1 cut(s) 1437
RsaI GTAC 4 cut(s) 230, 499, 676, 1110
RsaNI GTAC 4 cut(s) 229, 498, 675, 1109
RseI CAYNNNNRTG 2 cut(s) 832, 891
SaqAI TTAA 5 cut(s) 521, 621, 711, 1187, 1404
SatI GCNGC 5 cut(s) 40, 444, 447, 1209, 1448
Sau3AI GATC 2 cut(s) 553, 1319
Sau96I GGNCC 5 cut(s) 45, 125, 456, 1046, 1474
SchI GAGTC 2 cut(s) 417, 1367
ScrFI CCNGG 4 cut(s) 69, 487, 1132, 1356
SduI GDGCHC 3 cut(s) 989, 1338, 1463
SexAI ACCWGGT 1 cut(s) 1354
SfaNI GCATC 8 cut(s) 247, 655, 869, 1126, 1154, 1229, 1255, 1493
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 5 cut(s) 45, 125, 456, 1046, 1474
SmiMI CAYNNNNRTG 2 cut(s) 832, 891
SmlI CTYRAG 2 cut(s) 412, 520
SmoI CTYRAG 2 cut(s) 412, 520
Sse9I AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
SsiI CCGC 1 cut(s) 1447
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 473, 732
StyD4I CCNGG 4 cut(s) 67, 485, 1130, 1354
StyI CCWWGG 3 cut(s) 309, 480, 978
TaaI ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
TaqI TCGA 1 cut(s) 1302
TasI AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
TatI WGTACW 1 cut(s) 497
TauI GCSGC 1 cut(s) 1450
TfiI GAWTC 3 cut(s) 388, 719, 1283
Tru1I TTAA 5 cut(s) 521, 621, 711, 1187, 1404
Tru9I TTAA 5 cut(s) 521, 621, 711, 1187, 1404
TscAI CASTG 2 cut(s) 949, 1119
TseI GCWGC 4 cut(s) 39, 443, 446, 1208
TspDTI ATGAA 5 cut(s) 234, 261, 711, 963, 1191
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 2 cut(s) 949, 1119
Tth111I GACNNNGTC 1 cut(s) 1437
Vha464I CTTAAG 1 cut(s) 520
VneI GTGCAC 1 cut(s) 1334
VpaK11BI GGWCC 5 cut(s) 45, 125, 456, 1046, 1474
XagI CCTNNNNNAGG 1 cut(s) 484
XapI RAATTY 2 cut(s) 595, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmnI GAANNNNTTC 2 cut(s) 700, 1198
XspI CTAG 2 cut(s) 473, 732
Zsp2I ATGCAT 2 cut(s) 1141, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.