MD07G1310500.v1.1

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015300
Physical Location & Seq
Forward (+)
36512567 .. 36515755
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1310500.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGTCTGTATCGGTTGTTGTTTCTCCTCCGCACATAATCGGCGCCACGAGTGCGGCCCTGGCCCTGGGCTGCTCTCCACCACTCTATTCTCCGCATTCCCCTCGCATTTCTGTCTTTTTATACCCCCCTTCCACTCTCCCCGCGCCTTATCCACCGCGTCCTTCTTCAAAAAATAGAGTTACTTCCTCCTCCTCCAGCTCCTCTGCCGCCTTTGCTTTTCTCCATCACACCCCCGCAACCGCAACCATGACCAGAGGGCCACGTGGGTTTCTCAGCGCCTCCTCCTCCTCCTGCTGCTGCTGTAGCTTTAGAGCTCGCTCACCTGATCACTTTGAGAGCGCTGATGACGATGATGAAGATGAAAGTCCTTCTGCTGATACTGATGAGGAGGAAGAAGAATATGCCGACGGGGATTTTGGGTCGCCTGATAGATGGGACGTTTTGGGCCTGGGACAAGCCATGGTCGACTTTTCTGGGACTGTTGATGACGAGTTCCTGGAGAAATTGGGATTGGAAAAGGGAACAAGGAAGCTTGTGAATCATGAAGAGAGAGGTAGAGTTTTGAGAGCTATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGGTCTCTTTCCAACACTTTAGTGGCCTTGGCAAGGCTTGGTAGTGGCTCCATTGCAGGCTCTACCTTGAATGTAGCTATGGCAGGGAGTGTCGGGAGTGATCCATTGGGTGATTTTTACAGGACCAAGTTACGTCGTGCGAATTTGCATTTTCTTTCTGAACCTATCAAGGATGGGACAACAGGGACAGTGATAGTTCTCACAACTTCAGATGCCCAGCGTACAATGCTTGCATATCAGGGTACATCTTCAACTGTTAATTATGATCCATGCTTGGCTAGCACAGTTTGCAATACAAAAATATTTGTAGTCGAGGGTTATTTATTTGAACTTCCTGATACAATCAAAACAATTACAAAAGCATGTGAAGTAGCACGCAAGAGCGGTGCCCTGGTTGCAGTTACAGCATCAGATGTCTCCTGCATTGAGAGACACTATGATGATTTCTGGGAAATTGTTGGAAACTATGCGGACATTGTTTTTGCAAACAGTGACGAAGCAAGAGCATTCTGCCATTTTTCCTCAAAGGAGAGCCCCGTTTCAGCTACAAGGTATCTGAGCCATTTTGTTCCCCTGTCATCAGTTACTGATGGACCAAGAGGGTCTTACATTGGTGTAAAAGGAGAAGCTGTATATATTCCTCCTTCTCCATGTGTACCAGCGGACACTTGTGGTGCTGGAGATGCATATGCGTCGGGAATTTTGTATGGTATTCTACGAGGAGTGTCAGATTTGAAAGGAATAGGTACTCTAGCGGCAAGGGTTGCAGCCACAGTGGTGGGGCAGCAGGGCACACGACTTAGGGTTCAAGATGCAGTAGAGTTGGCAGAATCATTTGCATGCCATGTTGATAGTTCCCGCATTAGATCAGACATTGGATCAGATCATTTATCCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

502

Amino Acids

53.0

Weight (kDa)

5.15

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 196 - 468 2.9e-37 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 41, 989
AccBSI CCGCTC 1 cut(s) 987
AccI GTMKAC 1 cut(s) 465
AccII CGCG 2 cut(s) 143, 157
AclWI GGATC 3 cut(s) 698, 863, 1489
AcsI RAATTY 2 cut(s) 745, 1302
AcuI CTGAAG 1 cut(s) 795
AcvI CACGTG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 826, 847, 1260, 1351
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 2 cut(s) 64, 636
AgsI TTSAA 6 cut(s) 168, 673, 855, 932, 1339, 1412
AjnI CCWGG 5 cut(s) 57, 63, 447, 495, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 2 cut(s) 623, 1379
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 3 cut(s) 612, 1024, 1027
AlwI GGATC 3 cut(s) 698, 863, 1489
AlwNI CAGNNNCTG 1 cut(s) 1190
Aor51HI AGCGCT 1 cut(s) 340
AoxI GGCC 5 cut(s) 54, 60, 257, 445, 627
ApeKI GCWGC 7 cut(s) 69, 294, 297, 593, 596, 1370, 1387
ApoI RAATTY 2 cut(s) 745, 1302
ArsI GACNNNNNNTTYG 4 cut(s) 398, 430, 1067, 1099
AspLEI GCGC 4 cut(s) 44, 145, 278, 341
AspS9I GGNCC 6 cut(s) 55, 61, 257, 445, 726, 1196
AsuHPI GGTGA 2 cut(s) 312, 725
AsuNHI GCTAGC 1 cut(s) 881
AvaII GGWCC 2 cut(s) 726, 1196
BaeGI GKGCMC 2 cut(s) 994, 1397
BanI GGYRCC 2 cut(s) 41, 989
BanII GRGCYC 2 cut(s) 316, 1139
BauI CACGAG 1 cut(s) 46
BbrPI CACGTG 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 7 cut(s) 56, 281, 284, 580, 583, 1382, 1399
BccI CCATC 5 cut(s) 231, 426, 569, 770, 1187
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 1278, 1312
BciT130I CCWGG 5 cut(s) 59, 65, 449, 497, 995
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 612, 1024, 1027
BfaI CTAG 2 cut(s) 882, 1355
BfmI CTRYAG 1 cut(s) 301
BfoI RGCGCY 3 cut(s) 45, 279, 342
Bme1390I CCNGG 5 cut(s) 59, 65, 449, 497, 995
Bme18I GGWCC 2 cut(s) 726, 1196
BmgT120I GGNCC 6 cut(s) 55, 61, 257, 445, 726, 1196
BmiI GGNNCC 3 cut(s) 43, 652, 991
BmrFI CCNGG 5 cut(s) 59, 65, 449, 497, 995
BmsI GCATC 4 cut(s) 805, 1019, 1276, 1405
BmtI GCTAGC 1 cut(s) 885
BpmI CTGGAG 3 cut(s) 178, 518, 1302
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 7 cut(s) 57, 63, 64, 448, 459, 630, 993
BsaXI ACNNNNNCTCC 2 cut(s) 1218, 1248
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 636
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 5 cut(s) 59, 65, 449, 497, 995
BseDI CCNNGG 7 cut(s) 57, 63, 64, 448, 459, 630, 993
BseGI GGATG 2 cut(s) 580, 781
BseLI CCNNNNNNNGG 2 cut(s) 64, 636
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 286, 1151
BseRI GAGGAG 9 cut(s) 15, 178, 181, 190, 271, 274, 277, 401, 1338
BseSI GKGCMC 2 cut(s) 994, 1397
BseXI GCAGC 7 cut(s) 56, 281, 284, 580, 583, 1382, 1399
BseYI CCCAGC 1 cut(s) 819
Bsh1236I CGCG 2 cut(s) 143, 157
BshFI GGCC 5 cut(s) 56, 62, 259, 447, 629
BshNI GGYRCC 2 cut(s) 41, 989
BsiHKAI GWGCWC 1 cut(s) 316
BslFI GGGAC 5 cut(s) 449, 465, 490, 793, 802
BslI CCNNNNNNNGG 2 cut(s) 64, 636
BsmAI GTCTC 3 cut(s) 612, 1024, 1027
BsmFI GGGAC 5 cut(s) 449, 465, 490, 793, 802
BsmI GAATGC 2 cut(s) 94, 1109
BsnI GGCC 5 cut(s) 56, 62, 259, 447, 629
Bso31I GGTCTC 1 cut(s) 612
Bsp1286I GDGCHC 4 cut(s) 316, 994, 1139, 1397
Bsp143I GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
Bsp19I CCATGG 1 cut(s) 459
BspANI GGCC 5 cut(s) 56, 62, 259, 447, 629
BspCNI CTCAG 2 cut(s) 285, 1152
BspFNI CGCG 2 cut(s) 143, 157
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 3 cut(s) 43, 652, 991
BspOI GCTAGC 1 cut(s) 885
BspPI GGATC 3 cut(s) 698, 863, 1489
BspT107I GGYRCC 2 cut(s) 41, 989
BspTNI GGTCTC 1 cut(s) 612
BsrBI CCGCTC 1 cut(s) 987
BsrDI GCAATG 1 cut(s) 654
BssECI CCNNGG 7 cut(s) 57, 63, 64, 448, 459, 630, 993
BssMI GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 46
BssT1I CCWWGG 2 cut(s) 459, 630
Bst2BI CACGAG 1 cut(s) 46
Bst2UI CCWGG 5 cut(s) 59, 65, 449, 497, 995
Bst4CI ACNGT 6 cut(s) 481, 793, 859, 889, 1094, 1378
Bst6I CTCTTC 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 891, 1367
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 6 cut(s) 316, 661, 834, 883, 979, 1444
BstDEI CTNAG 3 cut(s) 272, 1160, 1403
BstDSI CCRYGG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 634
BstF5I GGATG 2 cut(s) 580, 781
BstFNI CGCG 2 cut(s) 143, 157
BstH2I RGCGCY 3 cut(s) 45, 279, 342
BstHHI GCGC 4 cut(s) 44, 145, 278, 341
BstKTI GATC 6 cut(s) 328, 706, 871, 1472, 1484, 1489
BstMAI GTCTC 3 cut(s) 612, 1024, 1027
BstMBI GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
BstNI CCWGG 5 cut(s) 59, 65, 449, 497, 995
BstNSI RCATGY 2 cut(s) 969, 1446
BstSCI CCNGG 5 cut(s) 57, 63, 447, 495, 993
BstSFI CTRYAG 1 cut(s) 301
BstSLI GKGCMC 2 cut(s) 994, 1397
BstUI CGCG 2 cut(s) 143, 157
BstV1I GCAGC 7 cut(s) 56, 281, 284, 580, 583, 1382, 1399
BstXI CCANNNNNNTGG 1 cut(s) 1381
BsuRI GGCC 5 cut(s) 56, 62, 259, 447, 629
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 580, 781
BtsIMutI CAGTG 3 cut(s) 798, 1099, 1383
Cac8I GCNNGC 6 cut(s) 316, 661, 834, 883, 979, 1444
CaiI CAGNNNCTG 1 cut(s) 1190
CciI TCATGA 1 cut(s) 541
CfoI GCGC 4 cut(s) 44, 145, 278, 341
Cfr13I GGNCC 6 cut(s) 55, 61, 257, 445, 726, 1196
CseI GACGC 2 cut(s) 146, 1284
Csp6I GTAC 4 cut(s) 825, 846, 1259, 1350
CviAII CATG 8 cut(s) 247, 460, 542, 873, 966, 1254, 1443, 1448
CviQI GTAC 4 cut(s) 825, 846, 1259, 1350
DdeI CTNAG 3 cut(s) 272, 1160, 1403
DinI GGCGCC 1 cut(s) 43
DpnI GATC 6 cut(s) 327, 705, 870, 1471, 1483, 1488
DpnII GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 2 cut(s) 459, 630
Eco24I GRGCYC 2 cut(s) 316, 1139
Eco31I GGTCTC 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 726, 1196
Eco47III AGCGCT 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 795
Eco72I CACGTG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 314
EcoNI CCTNNNNNAGG 1 cut(s) 634
EcoRII CCWGG 5 cut(s) 57, 63, 447, 495, 993
EcoT14I CCWWGG 2 cut(s) 459, 630
EcoT22I ATGCAT 1 cut(s) 1291
EcoT38I GRGCYC 2 cut(s) 316, 1139
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 459, 630
FaeI CATG 8 cut(s) 250, 463, 545, 876, 969, 1257, 1446, 1451
FalI AAGNNNNNCTT 2 cut(s) 1192, 1224
FaqI GGGAC 5 cut(s) 449, 465, 490, 793, 802
FatI CATG 8 cut(s) 246, 459, 541, 872, 965, 1253, 1442, 1447
FauI CCCGC 3 cut(s) 148, 241, 1469
FauNDI CATATG 1 cut(s) 1291
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 465
FokI GGATG 2 cut(s) 587, 788
FriOI GRGCYC 2 cut(s) 316, 1139
FspBI CTAG 2 cut(s) 882, 1355
GlaI GCGC 4 cut(s) 43, 144, 277, 340
GsaI CCCAGC 1 cut(s) 823
GsuI CTGGAG 3 cut(s) 178, 518, 1302
HaeII RGCGCY 3 cut(s) 45, 279, 342
HaeIII GGCC 5 cut(s) 56, 62, 259, 447, 629
HgaI GACGC 2 cut(s) 146, 1284
HhaI GCGC 4 cut(s) 44, 145, 278, 341
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 8 cut(s) 250, 463, 545, 876, 969, 1257, 1446, 1451
Hin6I GCGC 4 cut(s) 42, 143, 276, 339
HinP1I GCGC 4 cut(s) 42, 143, 276, 339
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 2 cut(s) 538, 1433
HphI GGTGA 2 cut(s) 312, 725
Hpy166II GTNNAC 2 cut(s) 466, 1259
Hpy188I TCNGA 7 cut(s) 763, 814, 1015, 1161, 1333, 1474, 1486
Hpy188III TCNNGA 5 cut(s) 542, 697, 938, 1299, 1412
Hpy8I GTNNAC 2 cut(s) 466, 1259
Hpy99I CGWCG 3 cut(s) 410, 741, 1300
HpyAV CCTTC 4 cut(s) 138, 171, 378, 1257
HpyCH4III ACNGT 6 cut(s) 481, 793, 859, 889, 1094, 1378
HpyCH4IV ACGT 3 cut(s) 262, 438, 736
HpyF3I CTNAG 3 cut(s) 272, 1160, 1403
HpySE526I ACGT 3 cut(s) 262, 438, 736
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 8 cut(s) 250, 463, 545, 876, 969, 1257, 1446, 1451
HspAI GCGC 4 cut(s) 42, 143, 276, 339
KasI GGCGCC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
LmnI GCTCC 2 cut(s) 203, 656
Lsp1109I GCAGC 7 cut(s) 56, 281, 284, 580, 583, 1382, 1399
LweI GCATC 4 cut(s) 805, 1019, 1276, 1405
MaeI CTAG 2 cut(s) 882, 1355
MaeII ACGT 3 cut(s) 262, 438, 736
MaeIII GTNAC 5 cut(s) 178, 732, 1003, 1094, 1186
MalI GATC 6 cut(s) 327, 705, 870, 1471, 1483, 1488
MbiI CCGCTC 1 cut(s) 987
MboI GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
MboII GAAGA 6 cut(s) 156, 368, 404, 407, 557, 843
MhlI GDGCHC 4 cut(s) 316, 994, 1139, 1397
MluCI AATT 6 cut(s) 503, 745, 862, 954, 1056, 1302
Mly113I GGCGCC 1 cut(s) 42
MmeI TCCRAC 2 cut(s) 639, 1042
Mph1103I ATGCAT 1 cut(s) 1291
MseI TTAA 1 cut(s) 861
MslI CAYNNNNRTG 4 cut(s) 623, 1041, 1379, 1441
MspA1I CMGCKG 1 cut(s) 1265
MspR9I CCNGG 5 cut(s) 59, 65, 449, 497, 995
Mva1269I GAATGC 2 cut(s) 94, 1109
MvaI CCWGG 5 cut(s) 59, 65, 449, 497, 995
MvnI CGCG 2 cut(s) 143, 157
NarI GGCGCC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 1291
NdeII GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
NheI GCTAGC 1 cut(s) 881
NlaIII CATG 8 cut(s) 250, 463, 545, 876, 969, 1257, 1446, 1451
NlaIV GGNNCC 3 cut(s) 43, 652, 991
NmuCI GTSAC 1 cut(s) 1094
NsiI ATGCAT 1 cut(s) 1291
NspI RCATGY 2 cut(s) 969, 1446
OliI CACNNNNGTG 2 cut(s) 623, 1379
PaeI GCATGC 1 cut(s) 1446
PagI TCATGA 1 cut(s) 541
PasI CCCWGGG 1 cut(s) 64
PctI GAATGC 2 cut(s) 94, 1109
PfeI GAWTC 2 cut(s) 538, 1433
PfoI TCCNGGA 1 cut(s) 495
PluTI GGCGCC 1 cut(s) 45
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 5 cut(s) 57, 63, 447, 495, 993
PspCI CACGTG 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 819
PspGI CCWGG 5 cut(s) 57, 63, 447, 495, 993
PspN4I GGNNCC 3 cut(s) 43, 652, 991
PspPI GGNCC 6 cut(s) 55, 61, 257, 445, 726, 1196
PstNI CAGNNNCTG 1 cut(s) 1190
RsaI GTAC 4 cut(s) 826, 847, 1260, 1351
RsaNI GTAC 4 cut(s) 825, 846, 1259, 1350
RseI CAYNNNNRTG 4 cut(s) 623, 1041, 1379, 1441
SacI GAGCTC 1 cut(s) 316
SalI GTCGAC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 861
Sau3AI GATC 6 cut(s) 325, 703, 868, 1469, 1481, 1486
Sau96I GGNCC 6 cut(s) 55, 61, 257, 445, 726, 1196
ScrFI CCNGG 5 cut(s) 59, 65, 449, 497, 995
SduI GDGCHC 4 cut(s) 316, 994, 1139, 1397
SfaNI GCATC 4 cut(s) 805, 1019, 1276, 1405
SfcI CTRYAG 1 cut(s) 301
SfoI GGCGCC 1 cut(s) 43
SinI GGWCC 2 cut(s) 726, 1196
SmiMI CAYNNNNRTG 4 cut(s) 623, 1041, 1379, 1441
SphI GCATGC 1 cut(s) 1446
Sse9I AATT 6 cut(s) 503, 745, 862, 954, 1056, 1302
SspDI GGCGCC 1 cut(s) 41
SspI AATATT 1 cut(s) 906
SspMI CTAG 2 cut(s) 882, 1355
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 5 cut(s) 57, 63, 447, 495, 993
StyI CCWWGG 2 cut(s) 459, 630
TaaI ACNGT 6 cut(s) 481, 793, 859, 889, 1094, 1378
TaiI ACGT 3 cut(s) 265, 441, 739
TaqI TCGA 2 cut(s) 465, 915
TasI AATT 6 cut(s) 503, 745, 862, 954, 1056, 1302
TauI GCSGC 3 cut(s) 56, 209, 1361
TfiI GAWTC 2 cut(s) 538, 1433
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 3 cut(s) 798, 1099, 1383
TseFI GTSAC 1 cut(s) 1094
TseI GCWGC 7 cut(s) 69, 294, 297, 593, 596, 1370, 1387
Tsp45I GTSAC 1 cut(s) 1094
TspDTI ATGAA 3 cut(s) 369, 375, 558
TspRI CASTG 3 cut(s) 798, 1099, 1383
VpaK11BI GGWCC 2 cut(s) 726, 1196
XagI CCTNNNNNAGG 1 cut(s) 634
XapI RAATTY 2 cut(s) 745, 1302
XceI RCATGY 2 cut(s) 969, 1446
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 2 cut(s) 882, 1355
Zsp2I ATGCAT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.