Rroxscaffold_4G00276620

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015300
Physical Location & Seq
Reverse (-)
175930 .. 179559
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276620.1

Sequence Viewer

Length: 1455 bp
ATGACGACCAGAGGTGCAGATTTTGAGACTAATGCTGATGACGATGAAGAAGAGTACGCATACATTGAAGATGAAGAATTAGATGCCGACCCTTTTTTGCCTGAGAGATGGGATGTTGTGGGTCTTGGACAAGCCATGGTGGACTTTTCCGGCATGGTTGATGATGAGTTTCTGGGAAAGCTGGATTTAGAAAAGGGAACAAGGAAGCTTGTGAATCACGAAGAGAGAGGTAGAGTCTTGAGAGCAATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGGTCCCTTTCCAACAGTCTAGTGGCCTTGGCCAGGCTTGGCAGTACATCCATTGGAGGCTCTCCCTTAAGTGTAGCTATGACAGGGAGTGTAGGGAGTGATCCGTTGGGTGGCTTCTACAGAGCAAAGTTACGTCGGGCAAATTTGCATTTTCTTTCTCCACCTATCAAAGATGGGACAACAGGGACAGTAATAGTTCTCACTTCTTCAGATACCCACCGTACAATGCTGGCATATCAGGGAACATCTTCAACTGTTAATTATGATTCATGCTTGGCTAGTGCAGTTTGCAATACAAAAATACTTGTAGTTGAGGGGTATTTGTTTGAACTTCCTGACACAATCAAAACAATTACAAAAGCCTGTGAAGAAGCACGCAGGTGTGGAGCGCTGGTCGCAGTTACAGCATCAGATGTTTCCTGCATTGAGAGACACTATGATGATTTCTGGGAAATTGTTGGGAACTATGCAGACATTGTTTTTGCAAACAGTGATGAAGCAAGAGCCTTCTGTCATTTTTCTTCCAAGGAGAGCCCCATTTCGGCTACAAGGTATCTCAGCCATTTTGTTCCATTCGCCTCGGTTACTGATGGACCAAGAGGCTCTTACATCGGTGTAAAAGGAGAAGCTGTATATATTCCTCCTTCTCCTTGTGTACCAGTGGACACTTGTGGTGCCGGGGATGCATATGCATCAGGAATTTTGTTTGGTATTTTACGAGGAGTGTCAGATTTAAAAGGAATAGGTACATTAGCAGCAAGGGTTGCATCCACGGTTGTTGGACAACAAGGCACTCGGCTTAGGGTTCAAGATGCAGTAGAGTTGGCAGAATCATTCACATTCCATCTCGATAGTTCCACTATTAGATCAGATATCGGTGAAGGTGCAGGGTGCACAAGCAGAAGGCGGCAAAGAAGGAACCTGGTGTTCACAAAGGGGACTCTGGGGCAGGGCTTTCAGAACCACACCTTAATACTGGAGAAGCTGATGAGAACCGGAGATGCTGTCTTTTGCGGCAAGACAAGTGCCCTCATCTCTGGACCCTACTGCATCCCCTTCGTCGTCAACCTCCTAGATGTCATCTTTGCTCGCGCATTGCCTCACATCAATGAGTTGGATGGAACCACTCGCAGACACTTCGACAGGATACCCCTAAAACTCGTCATCGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

484

Amino Acids

52.26

Weight (kDa)

5.33

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 88 - 360 1.9e-33 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 953
AccII CGCG 1 cut(s) 1371
AciI CCGC 2 cut(s) 1186, 1293
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 2 cut(s) 421, 978
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 5 cut(s) 56, 325, 502, 936, 1027
AfeI AGCGCT 1 cut(s) 669
AfiI CCNNNNNNNGG 3 cut(s) 312, 389, 820
AflII CTTAAG 1 cut(s) 346
AgsI TTSAA 4 cut(s) 68, 531, 608, 1088
AjnI CCWGG 2 cut(s) 311, 1200
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 6 cut(s) 181, 208, 261, 356, 908, 1264
AluI AGCT 6 cut(s) 181, 208, 261, 356, 908, 1264
Alw21I GWGCWC 1 cut(s) 1175
Alw26I GTCTC 2 cut(s) 20, 703
Alw44I GTGCAC 1 cut(s) 1171
AlwI GGATC 1 cut(s) 374
Aor51HI AGCGCT 1 cut(s) 669
AoxI GGCC 2 cut(s) 303, 309
ApaLI GTGCAC 1 cut(s) 1171
ApeKI GCWGC 3 cut(s) 269, 272, 1034
ApoI RAATTY 2 cut(s) 421, 978
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
Asp700I GAANNNNTTC 1 cut(s) 526
AspLEI GCGC 2 cut(s) 670, 1373
AspS9I GGNCC 3 cut(s) 282, 872, 1319
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 1 cut(s) 1169
AvaII GGWCC 3 cut(s) 282, 872, 1319
BaeGI GKGCMC 2 cut(s) 1175, 1309
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 1 cut(s) 815
Bbv12I GWGCWC 1 cut(s) 1175
BbvI GCAGC 3 cut(s) 256, 259, 1046
BccI CCATC 6 cut(s) 102, 245, 446, 863, 1131, 1391
BcgI CGANNNNNNTGC 4 cut(s) 1318, 1352, 1399, 1433
BciT130I CCWGG 2 cut(s) 313, 1202
BciVI GTATCC 1 cut(s) 1419
BcnI CCSGG 1 cut(s) 958
BcoDI GTCTC 2 cut(s) 20, 703
BfaI CTAG 3 cut(s) 299, 558, 1352
BfmI CTRYAG 1 cut(s) 397
BfoI RGCGCY 1 cut(s) 671
BfrI CTTAAG 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 648
BfuI GTATCC 1 cut(s) 1419
BisI GCNGC 5 cut(s) 270, 273, 1035, 1187, 1294
BlsI GCNGC 5 cut(s) 271, 274, 1036, 1188, 1295
Bme1390I CCNGG 3 cut(s) 313, 958, 1202
Bme18I GGWCC 3 cut(s) 282, 872, 1319
BmgT120I GGNCC 3 cut(s) 282, 872, 1319
BmiI GGNNCC 6 cut(s) 283, 284, 955, 1199, 1321, 1402
BmrFI CCNGG 3 cut(s) 313, 958, 1202
BmsI GCATC 8 cut(s) 73, 695, 952, 980, 1055, 1081, 1270, 1338
BpmI CTGGAG 1 cut(s) 1277
Bpu10I CCTNAGC 1 cut(s) 1079
BpuEI CTTGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 958
Bsa29I ATCGAT 1 cut(s) 1446
BsaJI CCNNGG 6 cut(s) 135, 306, 804, 858, 957, 1050
BsaWI WCCGGW 1 cut(s) 1274
BsaXI ACNNNNNCTCC 6 cut(s) 657, 687, 894, 924, 1269, 1299
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 389, 820
Bse1I ACTGG 2 cut(s) 938, 1260
Bse3DI GCAATG 2 cut(s) 252, 1373
BseBI CCWGG 2 cut(s) 313, 1202
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 6 cut(s) 135, 306, 804, 858, 957, 1050
BseGI GGATG 7 cut(s) 118, 256, 326, 967, 1046, 1329, 1402
BseLI CCNNNNNNNGG 3 cut(s) 312, 389, 820
BseMI GCAATG 2 cut(s) 252, 1373
BseMII CTCAG 2 cut(s) 93, 850
BseNI ACTGG 2 cut(s) 938, 1260
BseRI GAGGAG 1 cut(s) 1014
BseSI GKGCMC 2 cut(s) 1175, 1309
BseXI GCAGC 3 cut(s) 256, 259, 1046
BsgI GTGCAG 3 cut(s) 36, 582, 1185
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 2 cut(s) 305, 311
BshNI GGYRCC 1 cut(s) 953
BshVI ATCGAT 1 cut(s) 1446
BsiHKAI GWGCWC 1 cut(s) 1175
BsiSI CCGG 3 cut(s) 150, 957, 1275
BslFI GGGAC 4 cut(s) 268, 469, 478, 1231
BslI CCNNNNNNNGG 3 cut(s) 312, 389, 820
BsmAI GTCTC 2 cut(s) 20, 703
BsmFI GGGAC 4 cut(s) 268, 469, 478, 1231
BsnI GGCC 2 cut(s) 305, 311
Bsp1286I GDGCHC 3 cut(s) 815, 1175, 1309
Bsp143I GATC 3 cut(s) 379, 1145, 1447
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 2 cut(s) 1186, 1293
BspANI GGCC 2 cut(s) 305, 311
BspCNI CTCAG 2 cut(s) 94, 849
BspDI ATCGAT 1 cut(s) 1446
BspFNI CGCG 1 cut(s) 1371
BspLI GGNNCC 6 cut(s) 283, 284, 955, 1199, 1321, 1402
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 953
BspTI CTTAAG 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 252, 1373
BsrI ACTGG 2 cut(s) 938, 1260
BssECI CCNNGG 6 cut(s) 135, 306, 804, 858, 957, 1050
BssMI GATC 3 cut(s) 379, 1145, 1447
BssT1I CCWWGG 3 cut(s) 135, 306, 804
Bst2UI CCWGG 2 cut(s) 313, 1202
Bst4CI ACNGT 6 cut(s) 296, 469, 500, 535, 770, 1054
Bst6I CTCTTC 2 cut(s) 45, 216
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstC8I GCNNGC 3 cut(s) 510, 655, 1369
BstDEI CTNAG 3 cut(s) 102, 836, 1079
BstDSI CCRYGG 2 cut(s) 135, 1050
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 7 cut(s) 118, 256, 326, 967, 1046, 1329, 1402
BstFNI CGCG 1 cut(s) 1371
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 2 cut(s) 670, 1373
BstKTI GATC 3 cut(s) 382, 1148, 1450
BstMAI GTCTC 2 cut(s) 20, 703
BstMBI GATC 3 cut(s) 379, 1145, 1447
BstMWI GCNNNNNNNGC 4 cut(s) 674, 683, 962, 1043
BstNI CCWGG 2 cut(s) 313, 1202
BstSCI CCNGG 3 cut(s) 311, 956, 1200
BstSFI CTRYAG 1 cut(s) 397
BstSLI GKGCMC 2 cut(s) 1175, 1309
BstUI CGCG 1 cut(s) 1371
BstV1I GCAGC 3 cut(s) 256, 259, 1046
Bsu15I ATCGAT 1 cut(s) 1446
BsuI GTATCC 1 cut(s) 1419
BsuRI GGCC 2 cut(s) 305, 311
BsuTUI ATCGAT 1 cut(s) 1446
BtgI CCRYGG 2 cut(s) 135, 1050
BtsCI GGATG 7 cut(s) 118, 256, 326, 967, 1046, 1329, 1402
BtsIMutI CAGTG 2 cut(s) 775, 945
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 3 cut(s) 510, 655, 1369
CfoI GCGC 2 cut(s) 670, 1373
Cfr13I GGNCC 3 cut(s) 282, 872, 1319
ClaI ATCGAT 1 cut(s) 1446
CsiI ACCWGGT 1 cut(s) 1200
Csp6I GTAC 5 cut(s) 55, 324, 501, 935, 1026
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 3 cut(s) 136, 154, 549
CviQI GTAC 5 cut(s) 55, 324, 501, 935, 1026
DdeI CTNAG 3 cut(s) 102, 836, 1079
DpnI GATC 3 cut(s) 381, 1147, 1449
DpnII GATC 3 cut(s) 379, 1145, 1447
DraI TTTAAA 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 45, 216
EarI CTCTTC 2 cut(s) 45, 216
Eco130I CCWWGG 3 cut(s) 135, 306, 804
Eco24I GRGCYC 1 cut(s) 815
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 3 cut(s) 282, 872, 1319
Eco47III AGCGCT 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 471
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 311, 1200
EcoRV GATATC 1 cut(s) 1153
EcoT14I CCWWGG 3 cut(s) 135, 306, 804
EcoT22I ATGCAT 2 cut(s) 967, 973
EcoT38I GRGCYC 1 cut(s) 815
ErhI CCWWGG 3 cut(s) 135, 306, 804
FaeI CATG 3 cut(s) 139, 157, 552
FalI AAGNNNNNCTT 2 cut(s) 868, 900
FaqI GGGAC 4 cut(s) 268, 469, 478, 1231
FatI CATG 3 cut(s) 135, 153, 548
FauNDI CATATG 1 cut(s) 967
Fnu4HI GCNGC 5 cut(s) 270, 273, 1035, 1187, 1294
FokI GGATG 7 cut(s) 125, 263, 313, 974, 1033, 1316, 1409
FriOI GRGCYC 1 cut(s) 815
Fsp4HI GCNGC 5 cut(s) 270, 273, 1035, 1187, 1294
FspBI CTAG 3 cut(s) 299, 558, 1352
GlaI GCGC 2 cut(s) 669, 1372
GluI GCNGC 5 cut(s) 270, 273, 1035, 1187, 1294
GsuI CTGGAG 1 cut(s) 1277
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 2 cut(s) 305, 311
HapII CCGG 3 cut(s) 150, 957, 1275
HhaI GCGC 2 cut(s) 670, 1373
Hin1II CATG 3 cut(s) 139, 157, 552
Hin6I GCGC 2 cut(s) 668, 1371
HinP1I GCGC 2 cut(s) 668, 1371
HincII GTYRAC 1 cut(s) 1345
HindII GTYRAC 1 cut(s) 1345
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 214, 234, 545, 1109, 1219
HpaII CCGG 3 cut(s) 150, 957, 1275
HphI GGTGA 1 cut(s) 1169
Hpy166II GTNNAC 6 cut(s) 142, 935, 943, 1173, 1209, 1345
Hpy188I TCNGA 5 cut(s) 490, 691, 1009, 1150, 1239
Hpy188III TCNNGA 7 cut(s) 218, 238, 614, 975, 1088, 1127, 1317
Hpy8I GTNNAC 6 cut(s) 142, 935, 943, 1173, 1209, 1345
Hpy99I CGWCG 2 cut(s) 417, 1343
HpyAV CCTTC 6 cut(s) 796, 933, 1154, 1176, 1188, 1345
HpyCH4III ACNGT 6 cut(s) 296, 469, 500, 535, 770, 1054
HpyCH4IV ACGT 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 4 cut(s) 674, 683, 962, 1043
HpyF3I CTNAG 3 cut(s) 102, 836, 1079
HpySE526I ACGT 1 cut(s) 412
Hsp92II CATG 3 cut(s) 139, 157, 552
HspAI GCGC 2 cut(s) 668, 1371
KflI GGGWCCC 1 cut(s) 282
Kzo9I GATC 3 cut(s) 379, 1145, 1447
LmnI GCTCC 1 cut(s) 665
Lsp1109I GCAGC 3 cut(s) 256, 259, 1046
LweI GCATC 8 cut(s) 73, 695, 952, 980, 1055, 1081, 1270, 1338
MabI ACCWGGT 1 cut(s) 1200
MaeI CTAG 3 cut(s) 299, 558, 1352
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 408, 679, 862
MalI GATC 3 cut(s) 381, 1147, 1449
MboI GATC 3 cut(s) 379, 1145, 1447
MboII GAAGA 9 cut(s) 59, 62, 80, 86, 233, 477, 519, 659, 792
MhlI GDGCHC 3 cut(s) 815, 1175, 1309
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 6 cut(s) 77, 421, 538, 630, 732, 978
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 243, 1213
MmeI TCCRAC 3 cut(s) 315, 1039, 1374
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 967, 973
MroXI GAANNNNTTC 1 cut(s) 526
MscI TGGCCA 1 cut(s) 311
MseI TTAA 4 cut(s) 347, 537, 1013, 1250
MslI CAYNNNNRTG 3 cut(s) 658, 717, 1386
Msp20I TGGCCA 1 cut(s) 311
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 3 cut(s) 150, 957, 1275
MspR9I CCNGG 3 cut(s) 313, 958, 1202
MvaI CCWGG 2 cut(s) 313, 1202
MvnI CGCG 1 cut(s) 1371
MwoI GCNNNNNNNGC 4 cut(s) 674, 683, 962, 1043
NciI CCSGG 1 cut(s) 958
NcoI CCATGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 967
NdeII GATC 3 cut(s) 379, 1145, 1447
NlaIII CATG 3 cut(s) 139, 157, 552
NlaIV GGNNCC 6 cut(s) 283, 284, 955, 1199, 1321, 1402
NmeAIII GCCGAG 1 cut(s) 1054
NsiI ATGCAT 2 cut(s) 967, 973
OliI CACNNNNGTG 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 214, 545, 1109
PkrI GCNGC 5 cut(s) 271, 274, 1036, 1188, 1295
PleI GAGTC 2 cut(s) 242, 1213
PpsI GAGTC 2 cut(s) 242, 1213
PpuMI RGGWCCY 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 311, 1200
PspGI CCWGG 2 cut(s) 311, 1200
PspN4I GGNNCC 6 cut(s) 283, 284, 955, 1199, 1321, 1402
PspPI GGNCC 3 cut(s) 282, 872, 1319
PspPPI RGGWCCY 1 cut(s) 282
RsaI GTAC 5 cut(s) 56, 325, 502, 936, 1027
RsaNI GTAC 5 cut(s) 55, 324, 501, 935, 1026
RseI CAYNNNNRTG 3 cut(s) 658, 717, 1386
SaqAI TTAA 4 cut(s) 347, 537, 1013, 1250
SatI GCNGC 5 cut(s) 270, 273, 1035, 1187, 1294
Sau3AI GATC 3 cut(s) 379, 1145, 1447
Sau96I GGNCC 3 cut(s) 282, 872, 1319
SchI GAGTC 2 cut(s) 243, 1213
ScrFI CCNGG 3 cut(s) 313, 958, 1202
SduI GDGCHC 3 cut(s) 815, 1175, 1309
SexAI ACCWGGT 1 cut(s) 1200
SfaNI GCATC 8 cut(s) 73, 695, 952, 980, 1055, 1081, 1270, 1338
SfcI CTRYAG 1 cut(s) 397
SinI GGWCC 3 cut(s) 282, 872, 1319
SmiMI CAYNNNNRTG 3 cut(s) 658, 717, 1386
SmlI CTYRAG 2 cut(s) 238, 346
SmoI CTYRAG 2 cut(s) 238, 346
Sse9I AATT 6 cut(s) 77, 421, 538, 630, 732, 978
SsiI CCGC 2 cut(s) 1186, 1293
SspMI CTAG 3 cut(s) 299, 558, 1352
StyD4I CCNGG 3 cut(s) 311, 956, 1200
StyI CCWWGG 3 cut(s) 135, 306, 804
TaaI ACNGT 6 cut(s) 296, 469, 500, 535, 770, 1054
TaiI ACGT 1 cut(s) 415
TaqI TCGA 3 cut(s) 1128, 1419, 1446
TasI AATT 6 cut(s) 77, 421, 538, 630, 732, 978
TatI WGTACW 1 cut(s) 323
TauI GCSGC 2 cut(s) 1189, 1296
TfiI GAWTC 3 cut(s) 214, 545, 1109
Tru1I TTAA 4 cut(s) 347, 537, 1013, 1250
Tru9I TTAA 4 cut(s) 347, 537, 1013, 1250
TscAI CASTG 2 cut(s) 775, 945
TseI GCWGC 3 cut(s) 269, 272, 1034
TspDTI ATGAA 4 cut(s) 60, 87, 537, 789
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 2 cut(s) 775, 945
Vha464I CTTAAG 1 cut(s) 346
VneI GTGCAC 1 cut(s) 1171
VpaK11BI GGWCC 3 cut(s) 282, 872, 1319
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 2 cut(s) 421, 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 3 cut(s) 299, 558, 1352
Zsp2I ATGCAT 2 cut(s) 967, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.