Rh1CG445000

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0015300
Physical Location & Seq
Forward (+)
69654548 .. 69657492
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG445000.1

Sequence Viewer

Length: 1356 bp
ATGTCTCTCTCCTCTTCCTTTGTTCCCCAGATAATCGGCAGCAGGTCCTCTCCACCATTCTATTCTCCTGGTTTTCCTCTCCAGCTTCCTCGCGTTTCTCTCTTTTTATGCCCCTCATCTTTGGGTCCACCACATCTCCATCACAAAGCCACCACAAAGAATATTGTTGCTTCTATGACGACCAGAGGTGCAGATTTTGAGACTAATGCTGATGACGATGAAGAAGAGTACGCATACATTGAAGATGAAGAATTAGATGCCGACCCTTTTTTGCCTGAGAGATGGGATGTTGTGGGTCTTGGACAAGCCATGGTGGACTTTTCCGGCAAGGTTGATGATGAGTTTCTGGAAAAACTGGATTTAGAAAAGGGAACAAGGAAGCTTGTGAATCACGAAGAGAGAGGTAGAGTCTTGAGAGCAATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGGTCCCTTTCCAACAGTCTAGTGGCCTTGGCCAGGCTTGGCAGTACATCCATTGGAGGCTCTCCCTTAAGTGTAGCTATGACAGGGAGTGTAGGGAGTGATCCGTTGGGTGGCTTCTACAGAGCAAAGTTACGTCGGGCAAATTTGCATTTTCTTTCTCCACCTATCAAAGATGGGACAACAGGGACAGTAATAGTTCTCACTTCTTCAGATGCCCACCGTACAATGCTGGCATATCAGGGAACATCTTCGACTGTTAATTATGATTCATGCTTGGCTAGTGCAGTTTGCAATACAAAAATACTTGTAGTTGAGGGGTATTTGTTTGAACTTCCTGATACAATCAAAACAATTACAAAAGCCTGTGAAGAAGCACGCAGGTGTGGAGCGCTGGTCGCAGTTACAGCATCAGATGTTTCCTGCATTGAGAGACACTATGATGATTTCTGGGAAATTGTTGGGAACTATGCAGACATTGTTTTTGCAAACAGTGATGAAGCAAGAGCCTTCTGTCATTTTTCTTCGAAGGAGAGCCCCATTTCGGCTACAAGGTATCTCAGCCATTTTGTTCCATTCGCCTCGGTTACTGATGGACCAAGAGGCTCTTACATCGGTGTAAAAGGAGAAGCTGTATATATTCCTCCTTCTCCTTGTGTACCAGTGGACACTTGTGGTGCCGGGGATGCATATGCTTCAGGAATTTTGTTTGGTATTTTACGAGGAGTGTCAGATTTAAAAGGAATAGGTACATTAGCAGCAAGGGTTGCATCCACGGTTGTTGGACAACAAGGCACTCGGCTTAGGGTTCAAGATGCAGTAGAGTTGGCAGAATCATTCACATTCCATCTCGATAGTTCCACTATTAGATCAGATATCGGGTCAGATCATATTTCCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

451

Amino Acids

48.3

Weight (kDa)

5.14

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 146 - 418 1.1e-33 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 822
Acc36I ACCTGC 2 cut(s) 33, 822
AccB1I GGYRCC 1 cut(s) 1127
AccII CGCG 1 cut(s) 93
AclWI GGATC 1 cut(s) 548
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 2 cut(s) 595, 1152
AcuI CTGAAG 2 cut(s) 645, 1131
AfaI GTAC 5 cut(s) 230, 499, 676, 1110, 1201
AfeI AGCGCT 1 cut(s) 843
AfiI CCNNNNNNNGG 3 cut(s) 486, 563, 994
AflII CTTAAG 1 cut(s) 520
AgsI TTSAA 3 cut(s) 242, 782, 1262
AjnI CCWGG 2 cut(s) 67, 485
AleI CACNNNNGTG 1 cut(s) 832
AluBI AGCT 6 cut(s) 85, 382, 435, 530, 1082, 1350
AluI AGCT 6 cut(s) 85, 382, 435, 530, 1082, 1350
Alw26I GTCTC 3 cut(s) 9, 194, 877
AlwI GGATC 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 843
AoxI GGCC 2 cut(s) 477, 483
ApeKI GCWGC 4 cut(s) 39, 443, 446, 1208
ApoI RAATTY 2 cut(s) 595, 1152
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
Asp700I GAANNNNTTC 1 cut(s) 700
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 4 cut(s) 45, 125, 456, 1046
AsuC2I CCSGG 1 cut(s) 1132
AsuII TTCGAA 1 cut(s) 977
AvaII GGWCC 4 cut(s) 45, 125, 456, 1046
BalI TGGCCA 1 cut(s) 485
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 1 cut(s) 989
BbvI GCAGC 4 cut(s) 51, 430, 433, 1220
BccI CCATC 6 cut(s) 147, 276, 419, 620, 1037, 1305
BciT130I CCWGG 2 cut(s) 69, 487
BcnI CCSGG 1 cut(s) 1132
BcoDI GTCTC 3 cut(s) 9, 194, 877
BfaI CTAG 2 cut(s) 473, 732
BfmI CTRYAG 1 cut(s) 571
BfoI RGCGCY 1 cut(s) 845
BfrI CTTAAG 1 cut(s) 520
BfuAI ACCTGC 2 cut(s) 33, 822
BisI GCNGC 4 cut(s) 40, 444, 447, 1209
BlsI GCNGC 4 cut(s) 41, 445, 448, 1210
Bme1390I CCNGG 3 cut(s) 69, 487, 1132
Bme18I GGWCC 4 cut(s) 45, 125, 456, 1046
BmgT120I GGNCC 4 cut(s) 45, 125, 456, 1046
BmiI GGNNCC 4 cut(s) 126, 457, 458, 1129
BmrFI CCNGG 3 cut(s) 69, 487, 1132
BmsI GCATC 6 cut(s) 247, 655, 869, 1126, 1229, 1255
BpmI CTGGAG 1 cut(s) 65
Bpu10I CCTNAGC 1 cut(s) 1253
Bpu14I TTCGAA 1 cut(s) 977
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 1132
BsaJI CCNNGG 5 cut(s) 309, 480, 1032, 1131, 1224
BsaXI ACNNNNNCTCC 6 cut(s) 120, 150, 831, 861, 1068, 1098
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 563, 994
Bse1I ACTGG 2 cut(s) 360, 1112
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 69, 487
BseDI CCNNGG 5 cut(s) 309, 480, 1032, 1131, 1224
BseGI GGATG 5 cut(s) 292, 430, 500, 1141, 1220
BseLI CCNNNNNNNGG 3 cut(s) 486, 563, 994
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 267, 1024
BseNI ACTGG 2 cut(s) 360, 1112
BseRI GAGGAG 1 cut(s) 1188
BseXI GCAGC 4 cut(s) 51, 430, 433, 1220
BsgI GTGCAG 2 cut(s) 210, 756
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 479, 485
BshNI GGYRCC 1 cut(s) 1127
BsiSI CCGG 2 cut(s) 324, 1131
BslFI GGGAC 3 cut(s) 442, 643, 652
BslI CCNNNNNNNGG 3 cut(s) 486, 563, 994
BsmAI GTCTC 3 cut(s) 9, 194, 877
BsmFI GGGAC 3 cut(s) 442, 643, 652
BsnI GGCC 2 cut(s) 479, 485
Bsp119I TTCGAA 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 989
Bsp143I GATC 3 cut(s) 553, 1319, 1336
Bsp19I CCATGG 1 cut(s) 309
BspANI GGCC 2 cut(s) 479, 485
BspCNI CTCAG 2 cut(s) 268, 1023
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 4 cut(s) 126, 457, 458, 1129
BspMI ACCTGC 2 cut(s) 33, 822
BspPI GGATC 1 cut(s) 548
BspT104I TTCGAA 1 cut(s) 977
BspT107I GGYRCC 1 cut(s) 1127
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 426
BsrI ACTGG 2 cut(s) 360, 1112
BssECI CCNNGG 5 cut(s) 309, 480, 1032, 1131, 1224
BssMI GATC 3 cut(s) 553, 1319, 1336
BssT1I CCWWGG 2 cut(s) 309, 480
Bst2UI CCWGG 2 cut(s) 69, 487
Bst4CI ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
Bst6I CTCTTC 3 cut(s) 19, 219, 390
BstAFI CTTAAG 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstBI TTCGAA 1 cut(s) 977
BstC8I GCNNGC 2 cut(s) 684, 829
BstDEI CTNAG 3 cut(s) 276, 1010, 1253
BstDSI CCRYGG 2 cut(s) 309, 1224
BstENI CCTNNNNNAGG 1 cut(s) 484
BstF5I GGATG 5 cut(s) 292, 430, 500, 1141, 1220
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 3 cut(s) 556, 1322, 1339
BstMAI GTCTC 3 cut(s) 9, 194, 877
BstMBI GATC 3 cut(s) 553, 1319, 1336
BstMWI GCNNNNNNNGC 4 cut(s) 848, 857, 1136, 1217
BstNI CCWGG 2 cut(s) 69, 487
BstSCI CCNGG 3 cut(s) 67, 485, 1130
BstSFI CTRYAG 1 cut(s) 571
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 51, 430, 433, 1220
BsuRI GGCC 2 cut(s) 479, 485
BtgI CCRYGG 2 cut(s) 309, 1224
BtsCI GGATG 5 cut(s) 292, 430, 500, 1141, 1220
BtsIMutI CAGTG 2 cut(s) 949, 1119
BveI ACCTGC 2 cut(s) 33, 822
Cac8I GCNNGC 2 cut(s) 684, 829
CfoI GCGC 1 cut(s) 844
Cfr13I GGNCC 4 cut(s) 45, 125, 456, 1046
Csp6I GTAC 5 cut(s) 229, 498, 675, 1109, 1200
CspCI CAANNNNNGTGG 2 cut(s) 1213, 1248
CviAII CATG 2 cut(s) 310, 723
CviQI GTAC 5 cut(s) 229, 498, 675, 1109, 1200
DdeI CTNAG 3 cut(s) 276, 1010, 1253
DpnI GATC 3 cut(s) 555, 1321, 1338
DpnII GATC 3 cut(s) 553, 1319, 1336
DraI TTTAAA 1 cut(s) 1188
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 3 cut(s) 19, 219, 390
EarI CTCTTC 3 cut(s) 19, 219, 390
Eco130I CCWWGG 2 cut(s) 309, 480
Eco24I GRGCYC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 1327
Eco47I GGWCC 4 cut(s) 45, 125, 456, 1046
Eco47III AGCGCT 1 cut(s) 843
Eco57I CTGAAG 2 cut(s) 645, 1131
EcoNI CCTNNNNNAGG 1 cut(s) 484
EcoO109I RGGNCCY 2 cut(s) 45, 456
EcoRII CCWGG 2 cut(s) 67, 485
EcoRV GATATC 1 cut(s) 1327
EcoT14I CCWWGG 2 cut(s) 309, 480
EcoT22I ATGCAT 1 cut(s) 1141
EcoT38I GRGCYC 1 cut(s) 989
ErhI CCWWGG 2 cut(s) 309, 480
FaeI CATG 2 cut(s) 313, 726
FalI AAGNNNNNCTT 2 cut(s) 1042, 1074
FaqI GGGAC 3 cut(s) 442, 643, 652
FatI CATG 2 cut(s) 309, 722
FauNDI CATATG 1 cut(s) 1141
Fnu4HI GCNGC 4 cut(s) 40, 444, 447, 1209
FokI GGATG 5 cut(s) 299, 437, 487, 1148, 1207
FriOI GRGCYC 1 cut(s) 989
Fsp4HI GCNGC 4 cut(s) 40, 444, 447, 1209
FspBI CTAG 2 cut(s) 473, 732
GlaI GCGC 1 cut(s) 843
GluI GCNGC 4 cut(s) 40, 444, 447, 1209
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 2 cut(s) 479, 485
HapII CCGG 2 cut(s) 324, 1131
HhaI GCGC 1 cut(s) 844
Hin1II CATG 2 cut(s) 313, 726
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 4 cut(s) 388, 408, 719, 1283
HpaII CCGG 2 cut(s) 324, 1131
Hpy166II GTNNAC 4 cut(s) 128, 316, 1109, 1117
Hpy188I TCNGA 5 cut(s) 664, 865, 1183, 1324, 1336
Hpy188III TCNNGA 7 cut(s) 347, 392, 412, 788, 1149, 1262, 1301
Hpy8I GTNNAC 4 cut(s) 128, 316, 1109, 1117
Hpy99I CGWCG 1 cut(s) 591
HpyAV CCTTC 3 cut(s) 970, 973, 1107
HpyCH4III ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
HpyCH4IV ACGT 1 cut(s) 586
HpyF10VI GCNNNNNNNGC 4 cut(s) 848, 857, 1136, 1217
HpyF3I CTNAG 3 cut(s) 276, 1010, 1253
HpySE526I ACGT 1 cut(s) 586
Hsp92II CATG 2 cut(s) 313, 726
HspAI GCGC 1 cut(s) 842
KflI GGGWCCC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 553, 1319, 1336
LmnI GCTCC 1 cut(s) 839
Lsp1109I GCAGC 4 cut(s) 51, 430, 433, 1220
LweI GCATC 6 cut(s) 247, 655, 869, 1126, 1229, 1255
MaeI CTAG 2 cut(s) 473, 732
MaeII ACGT 1 cut(s) 586
MaeIII GTNAC 3 cut(s) 582, 853, 1036
MalI GATC 3 cut(s) 555, 1321, 1338
MboI GATC 3 cut(s) 553, 1319, 1336
MhlI GDGCHC 1 cut(s) 989
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 2 cut(s) 489, 1213
Mox20I TGGCCA 1 cut(s) 485
Mph1103I ATGCAT 1 cut(s) 1141
MroXI GAANNNNTTC 1 cut(s) 700
MscI TGGCCA 1 cut(s) 485
MseI TTAA 3 cut(s) 521, 711, 1187
MslI CAYNNNNRTG 2 cut(s) 832, 891
Msp20I TGGCCA 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 2 cut(s) 324, 1131
MspR9I CCNGG 3 cut(s) 69, 487, 1132
MvaI CCWGG 2 cut(s) 69, 487
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 848, 857, 1136, 1217
NciI CCSGG 1 cut(s) 1132
NcoI CCATGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 1141
NdeII GATC 3 cut(s) 553, 1319, 1336
NlaIII CATG 2 cut(s) 313, 726
NlaIV GGNNCC 4 cut(s) 126, 457, 458, 1129
NmeAIII GCCGAG 1 cut(s) 1228
NsiI ATGCAT 1 cut(s) 1141
NspV TTCGAA 1 cut(s) 977
OliI CACNNNNGTG 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 3 cut(s) 388, 719, 1283
PkrI GCNGC 4 cut(s) 41, 445, 448, 1210
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PpuMI RGGWCCY 2 cut(s) 45, 456
Psp5II RGGWCCY 2 cut(s) 45, 456
Psp6I CCWGG 2 cut(s) 67, 485
PspGI CCWGG 2 cut(s) 67, 485
PspN4I GGNNCC 4 cut(s) 126, 457, 458, 1129
PspPI GGNCC 4 cut(s) 45, 125, 456, 1046
PspPPI RGGWCCY 2 cut(s) 45, 456
RsaI GTAC 5 cut(s) 230, 499, 676, 1110, 1201
RsaNI GTAC 5 cut(s) 229, 498, 675, 1109, 1200
RseI CAYNNNNRTG 2 cut(s) 832, 891
SaqAI TTAA 3 cut(s) 521, 711, 1187
SatI GCNGC 4 cut(s) 40, 444, 447, 1209
Sau3AI GATC 3 cut(s) 553, 1319, 1336
Sau96I GGNCC 4 cut(s) 45, 125, 456, 1046
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 3 cut(s) 69, 487, 1132
SduI GDGCHC 1 cut(s) 989
SfaNI GCATC 6 cut(s) 247, 655, 869, 1126, 1229, 1255
SfcI CTRYAG 1 cut(s) 571
SfuI TTCGAA 1 cut(s) 977
SinI GGWCC 4 cut(s) 45, 125, 456, 1046
SmiMI CAYNNNNRTG 2 cut(s) 832, 891
SmlI CTYRAG 2 cut(s) 412, 520
SmoI CTYRAG 2 cut(s) 412, 520
Sse9I AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 473, 732
StyD4I CCNGG 3 cut(s) 67, 485, 1130
StyI CCWWGG 2 cut(s) 309, 480
TaaI ACNGT 6 cut(s) 470, 643, 674, 709, 944, 1228
TaiI ACGT 1 cut(s) 589
TaqI TCGA 3 cut(s) 704, 977, 1302
TasI AATT 6 cut(s) 251, 595, 712, 804, 906, 1152
TatI WGTACW 1 cut(s) 497
TfiI GAWTC 3 cut(s) 388, 719, 1283
Tru1I TTAA 3 cut(s) 521, 711, 1187
Tru9I TTAA 3 cut(s) 521, 711, 1187
TscAI CASTG 2 cut(s) 949, 1119
TseI GCWGC 4 cut(s) 39, 443, 446, 1208
TspDTI ATGAA 4 cut(s) 234, 261, 711, 963
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 2 cut(s) 949, 1119
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 4 cut(s) 45, 125, 456, 1046
XagI CCTNNNNNAGG 1 cut(s) 484
XapI RAATTY 2 cut(s) 595, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 2 cut(s) 473, 732
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.