RLG00000026034

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015300
Physical Location & Seq
Reverse (-)
153320 .. 157398
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026034

Sequence Viewer

Length: 1368 bp
ATGTCTCTCTCCTCTTCCTTTGTTGCTCCTTCTCCCCAGATAATCGGCAGCAGGTCCTCTCCACCAGTCTATTCTCCTGGTTTTCCTCTCCACCTTCCTCGAGTTTCTCTCTTTTTATACCCCTTATCATCTTTGGGTCCACCACATCTCCATCACAAAGCCACCACAAAGAATATTGTTGCTTCTATGACGACCAGAGGTGCACAATTTGAGACTGCTGCTGATGACGATGAAGAAGAGTACGTATGCATTGAGGATGAAGAATCAGATGCCGACCCTTTTTTGCCTGAGAGATGGGATGTTGTGGGTCTTGGACAAGCCATGGTGGACTTTTCCGGCATGGTTGATGATGAGTTTCTGGAAAAACTGGATTTAGAAAAGGGAACAAGGAAGCTTGTGAATCACGAAGAGAGAGGTAGAGTCTTGAGAGCAATGGATGGTTGTAGCTATAAGGCTGCTGCTGGTGGGTCCCTTTCCAACAGTCTAGTGGCCTTGGCCAGGCTTGGCAGTACATCCATTGGAGGCTCTCCCTTAAGTGTCGCTATGACAGGGAGTGTAGGGAGTGATCCGTTGGGTGGCTTCTACAGAGCAAAGTTACGTCGGGCAAATTTGCATTTTCTTTCTCCACCTATCAAAGATGGGACAACAGGGACAGTAATAGTTCTCACTTCTTCAGATGCCCACCGTACAATGCTGGCATATCAGGGAACATCTTCGACTGTTAATTATGATTCATGCTTGGCTAGTGCAGTTTGCAATACAAAAATACTTGTAGTTGAGGGGTATTTGTTTGAACTTCCTGATACAATCAAAACAATTACAAAAGCATGTGAAGAAGCACGCAGGTGTGGAGCACTGGTCGCAGTTACAGCATCAGATGTTTCCTGCATTGAGAGACACTATGATGATTTCTGGGAAATTGTTGGGAACTATGCAGACATTGTTTTTGCAAACAGTGACGAAGCGAGAGCCTTCTGTCATTTTTCTTCCAAGGAGAGCCCCATTTCGGCTACAAGGTATCTCAGCCATTTTGTTCCATTCGCCTCGGTTACTGATGGACCAAGAGGCTCTTACATCGGTGTAAAAGGAGAAGCTGTATATATTCCTCCTTCTCCTTGTGTACCAGTGGACACTTGTGGTGCCGGGGATGCATATGCATCCGGAATTTTGTTTGGTATTTTACGAGGAGTGTCAGATTTAAAAGGAATAGGTACATTAGCAGCTAGGGTTGCATCCACGGTTGTTGGACAACAAGGCACTCGGCTTAGGGTTCAAGATGCAGTAGAGTTGGCAGAATCATTCACATTCCATCTCGATAGTTCCACTATTAGATCAGATATCGGGTCAGATCATATATCCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

456

Amino Acids

48.6

Weight (kDa)

5.19

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 150 - 422 1.1e-33 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27600
fragaria_vesca FvH4_7g34180 FvH4_7g34180
malus_domestica MD07G1310500.v1.1
prunus_persica Prupe.2G328500_v2.0.a1
pyrus_communis pycom07g28270
rosa_chinensis RchiOBHm_Chr1g0384531
rosa_laevigata RLG00000026034
rosa_multiflora Rmu_sc0006005.1_g000007
rosa_roxburghii Rroxscaffold_4G00276620
rosa_rugosa Rorug01G0448700
rosa_samantha Rh1AG473800 Rh1BG431900 Rh1CG445000 Rh1DG464400
rosa_wichuraiana Rw1G039880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 834
Acc36I ACCTGC 2 cut(s) 42, 834
AccB1I GGYRCC 1 cut(s) 1139
AccIII TCCGGA 1 cut(s) 1160
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 2 cut(s) 607, 1164
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 5 cut(s) 242, 511, 688, 1122, 1213
AfiI CCNNNNNNNGG 3 cut(s) 498, 575, 1006
AflII CTTAAG 1 cut(s) 532
AgsI TTSAA 2 cut(s) 794, 1274
AjnI CCWGG 2 cut(s) 76, 497
AleI CACNNNNGTG 1 cut(s) 844
AluBI AGCT 5 cut(s) 394, 447, 1094, 1223, 1362
AluI AGCT 5 cut(s) 394, 447, 1094, 1223, 1362
Alw21I GWGCWC 2 cut(s) 205, 856
Alw26I GTCTC 3 cut(s) 9, 206, 889
Alw44I GTGCAC 1 cut(s) 201
AlwI GGATC 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 99
Aor13HI TCCGGA 1 cut(s) 1160
AoxI GGCC 2 cut(s) 489, 495
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 5 cut(s) 48, 218, 455, 458, 1220
ApoI RAATTY 2 cut(s) 607, 1164
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
Asp700I GAANNNNTTC 1 cut(s) 712
AspS9I GGNCC 4 cut(s) 54, 137, 468, 1058
AsuC2I CCSGG 1 cut(s) 1144
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 4 cut(s) 54, 137, 468, 1058
BaeGI GKGCMC 1 cut(s) 205
BalI TGGCCA 1 cut(s) 497
BanI GGYRCC 1 cut(s) 1139
BanII GRGCYC 1 cut(s) 1001
Bbv12I GWGCWC 2 cut(s) 205, 856
BbvI GCAGC 5 cut(s) 60, 205, 442, 445, 1232
BccI CCATC 6 cut(s) 159, 288, 431, 632, 1049, 1317
BciT130I CCWGG 2 cut(s) 78, 499
BcnI CCSGG 1 cut(s) 1144
BcoDI GTCTC 3 cut(s) 9, 206, 889
BfaI CTAG 3 cut(s) 485, 744, 1224
BfmI CTRYAG 1 cut(s) 583
BfrI CTTAAG 1 cut(s) 532
BfuAI ACCTGC 2 cut(s) 42, 834
BisI GCNGC 5 cut(s) 49, 219, 456, 459, 1221
BlsI GCNGC 5 cut(s) 50, 220, 457, 460, 1222
Bme1390I CCNGG 3 cut(s) 78, 499, 1144
Bme18I GGWCC 4 cut(s) 54, 137, 468, 1058
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 4 cut(s) 54, 137, 468, 1058
BmiI GGNNCC 4 cut(s) 138, 469, 470, 1141
BmrFI CCNGG 3 cut(s) 78, 499, 1144
BmsI GCATC 7 cut(s) 259, 667, 881, 1138, 1166, 1241, 1267
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 1265
BpuEI CTTGAG 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 1144
BsaAI YACGTR 1 cut(s) 244
BsaJI CCNNGG 6 cut(s) 321, 492, 990, 1044, 1143, 1236
BsaWI WCCGGW 1 cut(s) 1160
BsaXI ACNNNNNCTCC 6 cut(s) 132, 162, 843, 873, 1080, 1110
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 575, 1006
Bse1I ACTGG 4 cut(s) 65, 372, 861, 1124
Bse3DI GCAATG 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 1160
BseBI CCWGG 2 cut(s) 78, 499
BseDI CCNNGG 6 cut(s) 321, 492, 990, 1044, 1143, 1236
BseGI GGATG 7 cut(s) 262, 304, 442, 512, 1153, 1157, 1232
BseLI CCNNNNNNNGG 3 cut(s) 498, 575, 1006
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 279, 1036
BseNI ACTGG 4 cut(s) 65, 372, 861, 1124
BseRI GAGGAG 1 cut(s) 1200
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 5 cut(s) 60, 205, 442, 445, 1232
BsgI GTGCAG 1 cut(s) 768
BshFI GGCC 2 cut(s) 491, 497
BshNI GGYRCC 1 cut(s) 1139
BsiHKAI GWGCWC 2 cut(s) 205, 856
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 3 cut(s) 336, 1143, 1161
BslFI GGGAC 3 cut(s) 454, 655, 664
BslI CCNNNNNNNGG 3 cut(s) 498, 575, 1006
BsmAI GTCTC 3 cut(s) 9, 206, 889
BsmFI GGGAC 3 cut(s) 454, 655, 664
BsnI GGCC 2 cut(s) 491, 497
BsoBI CYCGRG 1 cut(s) 99
Bsp1286I GDGCHC 3 cut(s) 205, 856, 1001
Bsp13I TCCGGA 1 cut(s) 1160
Bsp143I GATC 3 cut(s) 565, 1331, 1348
Bsp19I CCATGG 1 cut(s) 321
BspANI GGCC 2 cut(s) 491, 497
BspCNI CTCAG 2 cut(s) 280, 1035
BspEI TCCGGA 1 cut(s) 1160
BspLI GGNNCC 4 cut(s) 138, 469, 470, 1141
BspMI ACCTGC 2 cut(s) 42, 834
BspPI GGATC 1 cut(s) 560
BspT107I GGYRCC 1 cut(s) 1139
BspTI CTTAAG 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 4 cut(s) 65, 372, 861, 1124
BssECI CCNNGG 6 cut(s) 321, 492, 990, 1044, 1143, 1236
BssMI GATC 3 cut(s) 565, 1331, 1348
BssT1I CCWWGG 3 cut(s) 321, 492, 990
Bst2UI CCWGG 2 cut(s) 78, 499
Bst4CI ACNGT 6 cut(s) 482, 655, 686, 721, 956, 1240
Bst6I CTCTTC 3 cut(s) 19, 231, 402
BstAFI CTTAAG 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 2 cut(s) 696, 841
BstDEI CTNAG 3 cut(s) 288, 1022, 1265
BstDSI CCRYGG 2 cut(s) 321, 1236
BstENI CCTNNNNNAGG 1 cut(s) 496
BstF5I GGATG 7 cut(s) 262, 304, 442, 512, 1153, 1157, 1232
BstKTI GATC 3 cut(s) 568, 1334, 1351
BstMAI GTCTC 3 cut(s) 9, 206, 889
BstMBI GATC 3 cut(s) 565, 1331, 1348
BstMWI GCNNNNNNNGC 4 cut(s) 860, 869, 1148, 1229
BstNI CCWGG 2 cut(s) 78, 499
BstNSI RCATGY 1 cut(s) 831
BstSCI CCNGG 3 cut(s) 76, 497, 1142
BstSFI CTRYAG 1 cut(s) 583
BstSLI GKGCMC 1 cut(s) 205
BstSNI TACGTA 1 cut(s) 244
BstV1I GCAGC 5 cut(s) 60, 205, 442, 445, 1232
BsuRI GGCC 2 cut(s) 491, 497
BtgI CCRYGG 2 cut(s) 321, 1236
BtsCI GGATG 7 cut(s) 262, 304, 442, 512, 1153, 1157, 1232
BtsIMutI CAGTG 3 cut(s) 854, 961, 1131
BveI ACCTGC 2 cut(s) 42, 834
Cac8I GCNNGC 2 cut(s) 696, 841
Cfr13I GGNCC 4 cut(s) 54, 137, 468, 1058
Csp6I GTAC 5 cut(s) 241, 510, 687, 1121, 1212
CspCI CAANNNNNGTGG 2 cut(s) 1225, 1260
CviAII CATG 4 cut(s) 322, 340, 735, 828
CviQI GTAC 5 cut(s) 241, 510, 687, 1121, 1212
DdeI CTNAG 3 cut(s) 288, 1022, 1265
DpnI GATC 3 cut(s) 567, 1333, 1350
DpnII GATC 3 cut(s) 565, 1331, 1348
DraI TTTAAA 1 cut(s) 1200
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 3 cut(s) 19, 231, 402
EarI CTCTTC 3 cut(s) 19, 231, 402
Eco105I TACGTA 1 cut(s) 244
Eco130I CCWWGG 3 cut(s) 321, 492, 990
Eco24I GRGCYC 1 cut(s) 1001
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 4 cut(s) 54, 137, 468, 1058
Eco57I CTGAAG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 496
EcoO109I RGGNCCY 2 cut(s) 54, 468
EcoRII CCWGG 2 cut(s) 76, 497
EcoRV GATATC 1 cut(s) 1339
EcoT14I CCWWGG 3 cut(s) 321, 492, 990
EcoT22I ATGCAT 3 cut(s) 251, 1153, 1159
EcoT38I GRGCYC 1 cut(s) 1001
ErhI CCWWGG 3 cut(s) 321, 492, 990
FaeI CATG 4 cut(s) 325, 343, 738, 831
FalI AAGNNNNNCTT 2 cut(s) 1054, 1086
FaqI GGGAC 3 cut(s) 454, 655, 664
FatI CATG 4 cut(s) 321, 339, 734, 827
FauNDI CATATG 1 cut(s) 1153
Fnu4HI GCNGC 5 cut(s) 49, 219, 456, 459, 1221
FokI GGATG 7 cut(s) 269, 311, 449, 499, 1144, 1160, 1219
FriOI GRGCYC 1 cut(s) 1001
Fsp4HI GCNGC 5 cut(s) 49, 219, 456, 459, 1221
FspBI CTAG 3 cut(s) 485, 744, 1224
GluI GCNGC 5 cut(s) 49, 219, 456, 459, 1221
HaeIII GGCC 2 cut(s) 491, 497
HapII CCGG 3 cut(s) 336, 1143, 1161
Hin1II CATG 4 cut(s) 325, 343, 738, 831
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 5 cut(s) 263, 400, 420, 731, 1295
HpaII CCGG 3 cut(s) 336, 1143, 1161
Hpy166II GTNNAC 5 cut(s) 140, 203, 328, 1121, 1129
Hpy188I TCNGA 6 cut(s) 268, 676, 877, 1195, 1336, 1348
Hpy188III TCNNGA 7 cut(s) 359, 404, 424, 800, 1161, 1274, 1313
Hpy8I GTNNAC 5 cut(s) 140, 203, 328, 1121, 1129
Hpy99I CGWCG 1 cut(s) 603
HpyAV CCTTC 4 cut(s) 39, 104, 982, 1119
HpyCH4III ACNGT 6 cut(s) 482, 655, 686, 721, 956, 1240
HpyCH4IV ACGT 2 cut(s) 243, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 860, 869, 1148, 1229
HpyF3I CTNAG 3 cut(s) 288, 1022, 1265
HpySE526I ACGT 2 cut(s) 243, 598
Hsp92II CATG 4 cut(s) 325, 343, 738, 831
KflI GGGWCCC 1 cut(s) 468
Kpn2I TCCGGA 1 cut(s) 1160
Kzo9I GATC 3 cut(s) 565, 1331, 1348
LmnI GCTCC 2 cut(s) 31, 851
Lsp1109I GCAGC 5 cut(s) 60, 205, 442, 445, 1232
LweI GCATC 7 cut(s) 259, 667, 881, 1138, 1166, 1241, 1267
MaeI CTAG 3 cut(s) 485, 744, 1224
MaeII ACGT 2 cut(s) 243, 598
MaeIII GTNAC 4 cut(s) 594, 865, 956, 1048
MalI GATC 3 cut(s) 567, 1333, 1350
MboI GATC 3 cut(s) 565, 1331, 1348
MboII GAAGA 9 cut(s) 6, 245, 248, 272, 419, 663, 705, 845, 978
MhlI GDGCHC 3 cut(s) 205, 856, 1001
MlsI TGGCCA 1 cut(s) 497
MluCI AATT 6 cut(s) 206, 607, 724, 816, 918, 1164
MluNI TGGCCA 1 cut(s) 497
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 2 cut(s) 501, 1225
Mox20I TGGCCA 1 cut(s) 497
Mph1103I ATGCAT 3 cut(s) 251, 1153, 1159
MroI TCCGGA 1 cut(s) 1160
MroXI GAANNNNTTC 1 cut(s) 712
MscI TGGCCA 1 cut(s) 497
MseI TTAA 3 cut(s) 533, 723, 1199
MslI CAYNNNNRTG 2 cut(s) 844, 903
Msp20I TGGCCA 1 cut(s) 497
MspCI CTTAAG 1 cut(s) 532
MspI CCGG 3 cut(s) 336, 1143, 1161
MspR9I CCNGG 3 cut(s) 78, 499, 1144
MvaI CCWGG 2 cut(s) 78, 499
MwoI GCNNNNNNNGC 4 cut(s) 860, 869, 1148, 1229
NciI CCSGG 1 cut(s) 1144
NcoI CCATGG 1 cut(s) 321
NdeI CATATG 1 cut(s) 1153
NdeII GATC 3 cut(s) 565, 1331, 1348
NlaIII CATG 4 cut(s) 325, 343, 738, 831
NlaIV GGNNCC 4 cut(s) 138, 469, 470, 1141
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 1 cut(s) 956
NsiI ATGCAT 3 cut(s) 251, 1153, 1159
NspI RCATGY 1 cut(s) 831
OliI CACNNNNGTG 1 cut(s) 844
PaeR7I CTCGAG 1 cut(s) 99
PaqCI CACCTGC 1 cut(s) 834
PdmI GAANNNNTTC 1 cut(s) 712
PfeI GAWTC 4 cut(s) 263, 400, 731, 1295
PkrI GCNGC 5 cut(s) 50, 220, 457, 460, 1222
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 244
PpuMI RGGWCCY 2 cut(s) 54, 468
Psp5II RGGWCCY 2 cut(s) 54, 468
Psp6I CCWGG 2 cut(s) 76, 497
PspGI CCWGG 2 cut(s) 76, 497
PspN4I GGNNCC 4 cut(s) 138, 469, 470, 1141
PspPI GGNCC 4 cut(s) 54, 137, 468, 1058
PspPPI RGGWCCY 2 cut(s) 54, 468
PspXI VCTCGAGB 1 cut(s) 99
RsaI GTAC 5 cut(s) 242, 511, 688, 1122, 1213
RsaNI GTAC 5 cut(s) 241, 510, 687, 1121, 1212
RseI CAYNNNNRTG 2 cut(s) 844, 903
SaqAI TTAA 3 cut(s) 533, 723, 1199
SatI GCNGC 5 cut(s) 49, 219, 456, 459, 1221
Sau3AI GATC 3 cut(s) 565, 1331, 1348
Sau96I GGNCC 4 cut(s) 54, 137, 468, 1058
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 3 cut(s) 78, 499, 1144
SduI GDGCHC 3 cut(s) 205, 856, 1001
SfaNI GCATC 7 cut(s) 259, 667, 881, 1138, 1166, 1241, 1267
SfcI CTRYAG 1 cut(s) 583
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 4 cut(s) 54, 137, 468, 1058
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 2 cut(s) 844, 903
SmlI CTYRAG 3 cut(s) 99, 424, 532
SmoI CTYRAG 3 cut(s) 99, 424, 532
SnaBI TACGTA 1 cut(s) 244
Sse9I AATT 6 cut(s) 206, 607, 724, 816, 918, 1164
SspI AATATT 1 cut(s) 175
SspMI CTAG 3 cut(s) 485, 744, 1224
StyD4I CCNGG 3 cut(s) 76, 497, 1142
StyI CCWWGG 3 cut(s) 321, 492, 990
TaaI ACNGT 6 cut(s) 482, 655, 686, 721, 956, 1240
TaiI ACGT 2 cut(s) 246, 601
TaqI TCGA 3 cut(s) 100, 716, 1314
TasI AATT 6 cut(s) 206, 607, 724, 816, 918, 1164
TatI WGTACW 1 cut(s) 509
TfiI GAWTC 4 cut(s) 263, 400, 731, 1295
Tru1I TTAA 3 cut(s) 533, 723, 1199
Tru9I TTAA 3 cut(s) 533, 723, 1199
TscAI CASTG 3 cut(s) 861, 961, 1131
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 5 cut(s) 48, 218, 455, 458, 1220
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 3 cut(s) 246, 273, 723
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 3 cut(s) 861, 961, 1131
Vha464I CTTAAG 1 cut(s) 532
VneI GTGCAC 1 cut(s) 201
VpaK11BI GGWCC 4 cut(s) 54, 137, 468, 1058
XagI CCTNNNNNAGG 1 cut(s) 496
XapI RAATTY 2 cut(s) 607, 1164
XceI RCATGY 1 cut(s) 831
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 712
XspI CTAG 3 cut(s) 485, 744, 1224
Zsp2I ATGCAT 3 cut(s) 251, 1153, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.