AT4G28850

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016167
Physical Location & Seq
Forward (+)
14244290 .. 14246037
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28850.1

Sequence Viewer

Length: 879 bp
ATGGCGGGTCTTCAAGCGAAGACGTTAATGTTCGTTCTGGCAGCTGCCTTAGCGACTCTTGGTCGCACATTTGTGGAGGCAGACTTCAGTAAGAATTTTATTGTTACATGGGGTAAAGATCACATGTTCATGAATGGTACCAACCTTCGACTTGTCCTAGACAAATCTGCAGGATCTGCTATTAAATCAAAGGTGGCTCATTTGTTTGGAAGCGTAGAAATGCTCATCAAACTTGTGCCAGGGAATTCTGCTGGAACCGTTGCAGCCTATTACTTATCGTCTACGGGAAGTACACACGATGAGATAGACTTCGAGTTCCTAGGAAATGCGACTGGTCAGCCGTACACGATCCACACCAACTTATATGCTCAAGGGAAAGGAAACCGCGAACAACAATTCCGGCCATGGTTCAACCCGACCAATGGTTTCCACAACTATACCATCCATTGGAACCCATCCGAAGTTGTGTGGTTCGTCGATGGAACTCCGATCAGAGTTTTTAGAAACTACGAAAGTGAAGGAATCGCATACCCGAACAAGCAAGGTATGAAAGTGTTTGCGAGTCTATGGAACGCAGAAGACTGGGCAACACAAGGAGGTCGAGTGAAGACTAACTGGACGCTAGCACCATTTGTGGCCGAGGGTCGTAGGTACAAGGCAAGAGCTTGTTTGTGGAAGGGATCAGTCAGCATCAAGCAATGTGTAGATCCAACTATACGGTCTAATTGGTGGACATCACCATCATTTAGTCAGCTCACTGCTTCGCAGCTGACGAAGATGCAAAAGATAAGAGATGGTTTCATGATCTATGATTATTGCAAGGACACTAACAGATTTAAAGGTGTTATGCCTCCGGAATGCTCGAAGAAGCAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

292

Amino Acids

32.92

Weight (kDa)

9.48

Isoelectric Point (pI)

26.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 30 - 207 2e-62 Glycosyl hydrolases family 16
XET_C PF06955 241 - 287 5.1e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28850
fragaria_vesca FvH4_7g07550
malus_domestica MD02G1057800.v1.1 MD15G1192200.v1.1
prunus_persica Prupe.7G225000_v2.0.a1
pyrus_communis pycom02g04690 pycom15g17210
rosa_chinensis RchiOBHm_Chr2g0091321
rosa_laevigata RLG00000016211
rosa_multiflora Rmu_sc0002270.1_g000048
rosa_roxburghii Rroxscaffold_2G00150210
rosa_rugosa Rorug02G0016300
rosa_samantha Rh2CG062600 Rh2DG061200
rosa_wichuraiana Rw2G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 447
AccI GTMKAC 1 cut(s) 281
AccII CGCG 1 cut(s) 387
AccIII TCCGGA 1 cut(s) 853
AciI CCGC 2 cut(s) 5, 385
AclWI GGATC 4 cut(s) 181, 343, 688, 701
AcoI YGGCCR 2 cut(s) 401, 636
AcsI RAATTY 2 cut(s) 94, 244
AcuI CTGAAG 1 cut(s) 70
AfaI GTAC 4 cut(s) 139, 292, 344, 653
AfiI CCNNNNNNNGG 2 cut(s) 422, 447
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 2 cut(s) 14, 412
AhdI GACNNNNNGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 238
AleI CACNNNNGTG 1 cut(s) 71
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
AluBI AGCT 4 cut(s) 44, 665, 754, 769
AluI AGCT 4 cut(s) 44, 665, 754, 769
AlwI GGATC 4 cut(s) 181, 343, 688, 701
AlwNI CAGNNNCTG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 853
AoxI GGCC 2 cut(s) 401, 636
ApeKI GCWGC 4 cut(s) 41, 44, 263, 766
ApoI RAATTY 2 cut(s) 94, 244
Asp700I GAANNNNTTC 1 cut(s) 872
Asp718I GGTACC 1 cut(s) 137
AspA2I CCTAGG 1 cut(s) 319
AsuHPI GGTGA 1 cut(s) 729
AsuNHI GCTAGC 1 cut(s) 622
AvrII CCTAGG 1 cut(s) 319
BanI GGYRCC 1 cut(s) 137
BbsI GAAGAC 4 cut(s) 2, 26, 585, 614
BbvI GCAGC 4 cut(s) 31, 53, 275, 778
BccI CCATC 5 cut(s) 449, 463, 473, 748, 788
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 240
BfaI CTAG 3 cut(s) 158, 320, 623
BfmI CTRYAG 1 cut(s) 168
BisI GCNGC 4 cut(s) 42, 45, 264, 767
BlnI CCTAGG 1 cut(s) 319
BlsI GCNGC 4 cut(s) 43, 46, 265, 768
Bme1390I CCNGG 1 cut(s) 240
BmeRI GACNNNNNGTC 1 cut(s) 60
BmiI GGNNCC 3 cut(s) 139, 256, 452
BmrFI CCNGG 1 cut(s) 240
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 2 cut(s) 699, 768
BmtI GCTAGC 1 cut(s) 626
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 4 cut(s) 2, 26, 585, 614
Bpu10I CCTNAGC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 354
BsaJI CCNNGG 4 cut(s) 239, 319, 404, 639
BsaWI WCCGGW 1 cut(s) 853
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 447
Bse1I ACTGG 3 cut(s) 337, 587, 620
Bse3DI GCAATG 1 cut(s) 704
BseAI TCCGGA 1 cut(s) 853
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 4 cut(s) 239, 319, 404, 639
BseGI GGATG 2 cut(s) 441, 455
BseLI CCNNNNNNNGG 2 cut(s) 422, 447
BseMI GCAATG 1 cut(s) 704
BseNI ACTGG 3 cut(s) 337, 587, 620
BseXI GCAGC 4 cut(s) 31, 53, 275, 778
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 2 cut(s) 403, 638
BshNI GGYRCC 1 cut(s) 137
BsiSI CCGG 2 cut(s) 400, 854
BslI CCNNNNNNNGG 2 cut(s) 422, 447
BsmI GAATGC 1 cut(s) 863
BsnI GGCC 2 cut(s) 403, 638
Bsp13I TCCGGA 1 cut(s) 853
Bsp143I GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 2 cut(s) 5, 385
BspANI GGCC 2 cut(s) 403, 638
BspEI TCCGGA 1 cut(s) 853
BspFNI CGCG 1 cut(s) 387
BspHI TCATGA 2 cut(s) 129, 801
BspLI GGNNCC 3 cut(s) 139, 256, 452
BspMAI CTGCAG 1 cut(s) 172
BspOI GCTAGC 1 cut(s) 626
BspPI GGATC 4 cut(s) 181, 343, 688, 701
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 704
BsrI ACTGG 3 cut(s) 337, 587, 620
BssECI CCNNGG 4 cut(s) 239, 319, 404, 639
BssMI GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
BssT1I CCWWGG 2 cut(s) 319, 404
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 259, 720
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 441, 455
BstFNI CGCG 1 cut(s) 387
BstKTI GATC 7 cut(s) 121, 176, 351, 492, 683, 709, 807
BstMBI GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
BstMWI GCNNNNNNNGC 2 cut(s) 50, 176
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 31, 53, 275, 778
BstV2I GAAGAC 4 cut(s) 2, 26, 585, 614
BstX2I RGATCY 2 cut(s) 173, 706
BstYI RGATCY 2 cut(s) 173, 706
BsuRI GGCC 2 cut(s) 403, 638
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 441, 455
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 1 cut(s) 624
CaiI CAGNNNCTG 1 cut(s) 176
CciI TCATGA 2 cut(s) 129, 801
CseI GACGC 1 cut(s) 628
Csp6I GTAC 4 cut(s) 138, 291, 343, 652
CviAII CATG 5 cut(s) 108, 124, 130, 405, 802
CviJI RGCY 9 cut(s) 44, 197, 266, 340, 403, 638, 665, 754, 769
CviKI_1 RGCY 9 cut(s) 44, 197, 266, 340, 403, 638, 665, 754, 769
CviQI GTAC 4 cut(s) 138, 291, 343, 652
DdeI CTNAG 1 cut(s) 49
DpnI GATC 7 cut(s) 120, 175, 350, 491, 682, 708, 806
DpnII GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
DraI TTTAAA 1 cut(s) 838
DriI GACNNNNNGTC 1 cut(s) 60
EaeI YGGCCR 2 cut(s) 401, 636
Eam1105I GACNNNNNGTC 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 319, 404
Eco57I CTGAAG 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 319, 404
ErhI CCWWGG 2 cut(s) 319, 404
FaeI CATG 5 cut(s) 111, 127, 133, 408, 805
FatI CATG 5 cut(s) 107, 123, 129, 404, 801
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 4 cut(s) 42, 45, 264, 767
FokI GGATG 2 cut(s) 428, 442
Fsp4HI GCNGC 4 cut(s) 42, 45, 264, 767
FspBI CTAG 3 cut(s) 158, 320, 623
GluI GCNGC 4 cut(s) 42, 45, 264, 767
HaeIII GGCC 2 cut(s) 403, 638
HapII CCGG 2 cut(s) 400, 854
HgaI GACGC 1 cut(s) 628
Hin1II CATG 5 cut(s) 111, 127, 133, 408, 805
HinfI GANTC 3 cut(s) 55, 522, 562
HpaII CCGG 2 cut(s) 400, 854
HphI GGTGA 1 cut(s) 729
Hpy166II GTNNAC 4 cut(s) 282, 293, 345, 732
Hpy188I TCNGA 3 cut(s) 460, 489, 494
Hpy188III TCNNGA 3 cut(s) 130, 802, 854
Hpy8I GTNNAC 4 cut(s) 282, 293, 345, 732
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 155, 512, 670
HpyCH4III ACNGT 2 cut(s) 259, 720
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 4 cut(s) 170, 263, 781, 819
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 176
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 5 cut(s) 111, 127, 133, 408, 805
Kpn2I TCCGGA 1 cut(s) 853
KpnI GGTACC 1 cut(s) 141
Kzo9I GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
Lsp1109I GCAGC 4 cut(s) 31, 53, 275, 778
LweI GCATC 2 cut(s) 699, 768
MaeI CTAG 3 cut(s) 158, 320, 623
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 103
MalI GATC 7 cut(s) 120, 175, 350, 491, 682, 708, 806
MboI GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
MboII GAAGA 5 cut(s) 31, 590, 619, 787, 877
MflI RGATCY 2 cut(s) 173, 706
MluCI AATT 5 cut(s) 94, 244, 395, 724, 872
MlyI GAGTC 2 cut(s) 49, 571
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 4 cut(s) 70, 590, 634, 861
MroI TCCGGA 1 cut(s) 853
MroXI GAANNNNTTC 1 cut(s) 872
MseI TTAA 3 cut(s) 26, 183, 837
MslI CAYNNNNRTG 2 cut(s) 71, 128
MspA1I CMGCKG 2 cut(s) 44, 769
MspI CCGG 2 cut(s) 400, 854
MspR9I CCNGG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 863
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 50, 176
NcoI CCATGG 1 cut(s) 404
NdeII GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
NheI GCTAGC 1 cut(s) 622
NlaIII CATG 5 cut(s) 111, 127, 133, 408, 805
NlaIV GGNNCC 3 cut(s) 139, 256, 452
NmeAIII GCCGAG 1 cut(s) 664
NspI RCATGY 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 71
PagI TCATGA 2 cut(s) 129, 801
PciI ACATGT 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 770
PctI GAATGC 1 cut(s) 863
PdmI GAANNNNTTC 1 cut(s) 872
PfeI GAWTC 1 cut(s) 522
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 4 cut(s) 43, 46, 265, 768
PleI GAGTC 2 cut(s) 49, 570
PpsI GAGTC 2 cut(s) 49, 570
PscI ACATGT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 139, 256, 452
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 2 cut(s) 173, 706
PvuII CAGCTG 2 cut(s) 44, 769
RsaI GTAC 4 cut(s) 139, 292, 344, 653
RsaNI GTAC 4 cut(s) 138, 291, 343, 652
RseI CAYNNNNRTG 2 cut(s) 71, 128
SaqAI TTAA 3 cut(s) 26, 183, 837
SatI GCNGC 4 cut(s) 42, 45, 264, 767
Sau3AI GATC 7 cut(s) 118, 173, 348, 489, 680, 706, 804
SchI GAGTC 2 cut(s) 49, 571
ScrFI CCNGG 1 cut(s) 240
SfaNI GCATC 2 cut(s) 699, 768
SfcI CTRYAG 1 cut(s) 168
SmiMI CAYNNNNRTG 2 cut(s) 71, 128
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
Sse9I AATT 5 cut(s) 94, 244, 395, 724, 872
SsiI CCGC 2 cut(s) 5, 385
SspMI CTAG 3 cut(s) 158, 320, 623
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 2 cut(s) 319, 404
TaaI ACNGT 2 cut(s) 259, 720
TaiI ACGT 1 cut(s) 26
TaqI TCGA 5 cut(s) 148, 312, 477, 601, 863
TasI AATT 5 cut(s) 94, 244, 395, 724, 872
TatI WGTACW 1 cut(s) 290
TfiI GAWTC 1 cut(s) 522
Tru1I TTAA 3 cut(s) 26, 183, 837
Tru9I TTAA 3 cut(s) 26, 183, 837
TscAI CASTG 1 cut(s) 763
TseI GCWGC 4 cut(s) 41, 44, 263, 766
TspDTI ATGAA 4 cut(s) 118, 146, 563, 790
TspRI CASTG 1 cut(s) 763
Van91I CCANNNNNTGG 1 cut(s) 447
XapI RAATTY 2 cut(s) 94, 244
XceI RCATGY 1 cut(s) 127
XmaJI CCTAGG 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 872
XspI CTAG 3 cut(s) 158, 320, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.