MD02G1057800.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016167
Physical Location & Seq
Forward (+)
4671892 .. 4673503
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1057800.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGCAGTTTTGCAAGCATTCCTGGTAGCTCTATGCATCACCGTGTTCCATCAAAATGTAGCTGATGCAACTGCTGCAAAGAGCATGTACCTCACCTGGGGTACCCAACACGCTGCGATTCAAGGCGAGAATCTTAACCTCGTGCTTGATCAAACTTCAGGGTCTGCTGCTATGTCAAAGAGAAATTTCTTATTCGGAAGCATCGAGATGCTGATAAAGTTGGTACCTGGGAATTCCGCCGGGACAGTAACAGCCTACTATCTATCGTCAACAGGAACCAAACACGATGAGATAGATTTTGAATTCCTAGGGAACGTTTCTGGACAACCTTACATTGTCCACACAAACATTTATACACAAGGAAATGGAAGCAAAGAGCAGCAATTTTACCTCTGGTTTGACCCGACCGCTGATTTCCACAACTACACCATACATTGGAACCCTACTGAAGTTGTGTGGTATGTCGATAGTTTGCCCATTCGTGTTTTCCGAAACTACGAGAACCAGGGGATTGCTTACCCCAATAAGCAAGGGATGAGGGTTTACTCCAGCTTATGGAATGCTGATAACTGGGCAACTAGAGGTGGGCTAGAGAAGATAAATTGGACCGGTGCGCCCTTCACAGCCGGATTCAAACACTTCAGGGCAAGAGCCTGCAAGTGGAATGGAGCTGCCAGCATTAGTGTATGTGCCGCCACTTCCCGGGCTAACTGGTGGACTTCCCCTATATACAGCCAGCTGAGCAACAGTAAGCTCGGCCAGCTGCAATGGGTCAGGAATAACTACATGATCTATGACTACTGCAAAGACAATAAACGATTCAATGGAAACATGCCACCTGAATGTTCTAAACAACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

288

Amino Acids

32.48

Weight (kDa)

8.7

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 203 2.9e-61 Glycosyl hydrolases family 16
XET_C PF06955 234 - 282 3.4e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28850
fragaria_vesca FvH4_7g07550
malus_domestica MD02G1057800.v1.1 MD15G1192200.v1.1
prunus_persica Prupe.7G225000_v2.0.a1
pyrus_communis pycom02g04690 pycom15g17210
rosa_chinensis RchiOBHm_Chr2g0091321
rosa_laevigata RLG00000016211
rosa_multiflora Rmu_sc0002270.1_g000048
rosa_roxburghii Rroxscaffold_2G00150210
rosa_rugosa Rorug02G0016300
rosa_samantha Rh2CG062600 Rh2DG061200
rosa_wichuraiana Rw2G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 101, 223
AccB1I GGYRCC 2 cut(s) 101, 223
AccB7I CCANNNNNTGG 2 cut(s) 435, 555
AciI CCGC 3 cut(s) 237, 408, 693
AclI AACGTT 1 cut(s) 315
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 3 cut(s) 184, 232, 302
AcuI CTGAAG 3 cut(s) 141, 468, 625
AfaI GTAC 3 cut(s) 89, 103, 225
AfiI CCNNNNNNNGG 5 cut(s) 97, 435, 555, 660, 702
AgeI ACCGGT 1 cut(s) 608
AgsI TTSAA 4 cut(s) 122, 302, 634, 823
AjnI CCWGG 4 cut(s) 21, 95, 226, 504
AluBI AGCT 7 cut(s) 29, 62, 552, 671, 739, 754, 763
AluI AGCT 7 cut(s) 29, 62, 552, 671, 739, 754, 763
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 757
ApeKI GCWGC 6 cut(s) 74, 113, 167, 379, 671, 763
ApoI RAATTY 3 cut(s) 184, 232, 302
AsiGI ACCGGT 1 cut(s) 608
Asp718I GGTACC 2 cut(s) 101, 223
AspA2I CCTAGG 1 cut(s) 307
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 1 cut(s) 606
AsuC2I CCSGG 3 cut(s) 241, 703, 704
AsuHPI GGTGA 2 cut(s) 31, 85
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 1 cut(s) 606
AvrII CCTAGG 1 cut(s) 307
BanI GGYRCC 2 cut(s) 101, 223
BauI CACGAG 1 cut(s) 140
BbvI GCAGC 6 cut(s) 61, 100, 154, 391, 658, 750
BccI CCATC 1 cut(s) 57
BciT130I CCWGG 4 cut(s) 23, 97, 228, 506
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 3 cut(s) 241, 703, 704
BfaI CTAG 3 cut(s) 308, 579, 590
BisI GCNGC 7 cut(s) 75, 114, 168, 380, 672, 693, 764
BlnI CCTAGG 1 cut(s) 307
BlpI GCTNAGC 1 cut(s) 740
BlsI GCNGC 7 cut(s) 76, 115, 169, 381, 673, 694, 765
Bme1390I CCNGG 7 cut(s) 23, 97, 228, 241, 506, 703, 704
Bme18I GGWCC 1 cut(s) 606
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 1 cut(s) 606
BmiI GGNNCC 4 cut(s) 103, 225, 277, 440
BmrFI CCNGG 7 cut(s) 23, 97, 228, 241, 506, 703, 704
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 4 cut(s) 45, 55, 198, 210
BmuI ACTGGG 1 cut(s) 580
BpmI CTGGAG 1 cut(s) 532
Bpu1102I GCTNAGC 1 cut(s) 740
BpuMI CCSGG 3 cut(s) 241, 703, 704
BsaJI CCNNGG 5 cut(s) 96, 227, 307, 505, 702
BsaWI WCCGGW 1 cut(s) 608
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 435, 555, 660, 702
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 2 cut(s) 575, 716
Bse3DI GCAATG 1 cut(s) 773
BseBI CCWGG 4 cut(s) 23, 97, 228, 506
BseDI CCNNGG 5 cut(s) 96, 227, 307, 505, 702
BseGI GGATG 1 cut(s) 540
BseLI CCNNNNNNNGG 5 cut(s) 97, 435, 555, 660, 702
BseMI GCAATG 1 cut(s) 773
BseMII CTCAG 1 cut(s) 731
BseNI ACTGG 2 cut(s) 575, 716
BseXI GCAGC 6 cut(s) 61, 100, 154, 391, 658, 750
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 1 cut(s) 759
BshNI GGYRCC 2 cut(s) 101, 223
BshTI ACCGGT 1 cut(s) 608
BsiEI CGRYCG 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 4 cut(s) 240, 609, 627, 703
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 5 cut(s) 97, 435, 555, 660, 702
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 17, 565
BsnI GGCC 1 cut(s) 759
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 2 cut(s) 148, 789
Bsp1720I GCTNAGC 1 cut(s) 740
BspACI CCGC 3 cut(s) 237, 408, 693
BspANI GGCC 1 cut(s) 759
BspCNI CTCAG 1 cut(s) 732
BspLI GGNNCC 4 cut(s) 103, 225, 277, 440
BspT107I GGYRCC 2 cut(s) 101, 223
BsrDI GCAATG 1 cut(s) 773
BsrFI RCCGGY 1 cut(s) 608
BsrI ACTGG 2 cut(s) 575, 716
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 5 cut(s) 96, 227, 307, 505, 702
BssMI GATC 2 cut(s) 148, 789
BssSI CACGAG 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 4 cut(s) 23, 97, 228, 506
Bst4CI ACNGT 3 cut(s) 43, 247, 749
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 15, 655, 676, 737, 761
BstDEI CTNAG 1 cut(s) 740
BstF5I GGATG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 2 cut(s) 151, 792
BstMBI GATC 2 cut(s) 148, 789
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 3 cut(s) 74, 741, 760
BstNI CCWGG 4 cut(s) 23, 97, 228, 506
BstNSI RCATGY 2 cut(s) 88, 835
BstSCI CCNGG 7 cut(s) 21, 95, 226, 239, 504, 701, 702
BstV1I GCAGC 6 cut(s) 61, 100, 154, 391, 658, 750
BsuRI GGCC 1 cut(s) 759
BtsCI GGATG 1 cut(s) 540
Cac8I GCNNGC 5 cut(s) 15, 655, 676, 737, 761
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 606
Cfr9I CCCGGG 1 cut(s) 702
Csp6I GTAC 3 cut(s) 88, 102, 224
CspAI ACCGGT 1 cut(s) 608
CviAII CATG 3 cut(s) 85, 787, 832
CviQI GTAC 3 cut(s) 88, 102, 224
DdeI CTNAG 1 cut(s) 740
DpnI GATC 2 cut(s) 150, 791
DpnII GATC 2 cut(s) 148, 789
EaeI YGGCCR 1 cut(s) 757
EciI GGCGGA 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 606
Eco57I CTGAAG 3 cut(s) 141, 468, 625
Eco88I CYCGRG 1 cut(s) 702
EcoRI GAATTC 2 cut(s) 232, 302
EcoRII CCWGG 4 cut(s) 21, 95, 226, 504
EcoT14I CCWWGG 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 3 cut(s) 88, 790, 835
FaqI GGGAC 1 cut(s) 256
FatI CATG 3 cut(s) 84, 786, 831
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 7 cut(s) 75, 114, 168, 380, 672, 693, 764
FokI GGATG 1 cut(s) 547
Fsp4HI GCNGC 7 cut(s) 75, 114, 168, 380, 672, 693, 764
FspBI CTAG 3 cut(s) 308, 579, 590
GlaI GCGC 1 cut(s) 615
GluI GCNGC 7 cut(s) 75, 114, 168, 380, 672, 693, 764
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 1 cut(s) 759
HapII CCGG 4 cut(s) 240, 609, 627, 703
HhaI GCGC 1 cut(s) 616
Hin1II CATG 3 cut(s) 88, 790, 835
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 4 cut(s) 118, 130, 630, 819
HpaII CCGG 4 cut(s) 240, 609, 627, 703
HphI GGTGA 2 cut(s) 31, 85
Hpy166II GTNNAC 4 cut(s) 270, 340, 544, 717
Hpy188I TCNGA 3 cut(s) 197, 491, 863
Hpy188III TCNNGA 3 cut(s) 205, 321, 775
Hpy8I GTNNAC 4 cut(s) 270, 340, 544, 717
HpyAV CCTTC 1 cut(s) 628
HpyCH4III ACNGT 3 cut(s) 43, 247, 749
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 7 cut(s) 13, 36, 68, 77, 657, 766, 804
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 741, 760
HpyF3I CTNAG 1 cut(s) 740
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 3 cut(s) 88, 790, 835
HspAI GCGC 1 cut(s) 614
KpnI GGTACC 2 cut(s) 105, 227
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 2 cut(s) 148, 789
LmnI GCTCC 1 cut(s) 668
Lsp1109I GCAGC 6 cut(s) 61, 100, 154, 391, 658, 750
LweI GCATC 4 cut(s) 45, 55, 198, 210
MaeI CTAG 3 cut(s) 308, 579, 590
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 150, 791
MboI GATC 2 cut(s) 148, 789
MboII GAAGA 1 cut(s) 607
MluCI AATT 6 cut(s) 184, 232, 302, 383, 601, 857
MnlI CCTC 5 cut(s) 101, 149, 401, 531, 575
Mph1103I ATGCAT 1 cut(s) 38
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 4 cut(s) 41, 54, 206, 841
MspA1I CMGCKG 3 cut(s) 410, 739, 763
MspI CCGG 4 cut(s) 240, 609, 627, 703
MspR9I CCNGG 7 cut(s) 23, 97, 228, 241, 506, 703, 704
Mva1269I GAATGC 2 cut(s) 17, 565
MvaI CCWGG 4 cut(s) 23, 97, 228, 506
MwoI GCNNNNNNNGC 3 cut(s) 74, 741, 760
NciI CCSGG 3 cut(s) 241, 703, 704
NdeII GATC 2 cut(s) 148, 789
NlaIII CATG 3 cut(s) 88, 790, 835
NlaIV GGNNCC 4 cut(s) 103, 225, 277, 440
NmeAIII GCCGAG 1 cut(s) 735
NsiI ATGCAT 1 cut(s) 38
NspI RCATGY 2 cut(s) 88, 835
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 487
PctI GAATGC 2 cut(s) 17, 565
PfeI GAWTC 4 cut(s) 118, 130, 630, 819
PflMI CCANNNNNTGG 2 cut(s) 435, 555
PinAI ACCGGT 1 cut(s) 608
PkrI GCNGC 7 cut(s) 76, 115, 169, 381, 673, 694, 765
Psp1406I AACGTT 1 cut(s) 315
Psp6I CCWGG 4 cut(s) 21, 95, 226, 504
PspGI CCWGG 4 cut(s) 21, 95, 226, 504
PspN4I GGNNCC 4 cut(s) 103, 225, 277, 440
PspPI GGNCC 1 cut(s) 606
PstNI CAGNNNCTG 1 cut(s) 164
PvuII CAGCTG 2 cut(s) 739, 763
RsaI GTAC 3 cut(s) 89, 103, 225
RsaNI GTAC 3 cut(s) 88, 102, 224
RseI CAYNNNNRTG 4 cut(s) 41, 54, 206, 841
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 7 cut(s) 75, 114, 168, 380, 672, 693, 764
Sau3AI GATC 2 cut(s) 148, 789
Sau96I GGNCC 1 cut(s) 606
ScrFI CCNGG 7 cut(s) 23, 97, 228, 241, 506, 703, 704
SfaNI GCATC 4 cut(s) 45, 55, 198, 210
SinI GGWCC 1 cut(s) 606
SmaI CCCGGG 1 cut(s) 704
SmiMI CAYNNNNRTG 4 cut(s) 41, 54, 206, 841
Sse9I AATT 6 cut(s) 184, 232, 302, 383, 601, 857
SsiI CCGC 3 cut(s) 237, 408, 693
SspMI CTAG 3 cut(s) 308, 579, 590
StyD4I CCNGG 7 cut(s) 21, 95, 226, 239, 504, 701, 702
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 3 cut(s) 43, 247, 749
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 204, 465
TasI AATT 6 cut(s) 184, 232, 302, 383, 601, 857
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 4 cut(s) 118, 130, 630, 819
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseI GCWGC 6 cut(s) 74, 113, 167, 379, 671, 763
TspMI CCCGGG 1 cut(s) 702
Van91I CCANNNNNTGG 2 cut(s) 435, 555
VpaK11BI GGWCC 1 cut(s) 606
XapI RAATTY 3 cut(s) 184, 232, 302
XceI RCATGY 2 cut(s) 88, 835
XmaI CCCGGG 1 cut(s) 702
XmaJI CCTAGG 1 cut(s) 307
XspI CTAG 3 cut(s) 308, 579, 590
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.