MD15G1192200.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016167
Physical Location & Seq
Forward (+)
15176219 .. 15177888
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1192200.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCACTAGCTTTGCAAGCATTTCTGGTAGCTCTGTGCATCACTGCGTTCCATCAAAATTTAGCCGATGCAAAAGTTTCGAAGAGCATGTACCTCACCTGGGGTACCCAACACGCTACGATTCAAGGCGAGAATCTTAACCTCGTGCTCGATCAAACTTCAGGGTCTGCTGCAATGTCAAAGAGAGGTTTCTTATTCGGAAGCATCGAGATGCTGATTAAGTTGGTACCTGGGAATTCCGCCGGGATAGTGACAGCGTACTATCTATCGTCAACAGGACCCAAACACGATGAGATAGATTTTGAATTCCTAGGGAACGTTTCTGGACAACCTTACATTGTCCACACAAACATATACACACAAGGAAATGGAACCAAAGAGCAGCAATTTTACCTCTGGTTTGACCCGACCGCGGATTTCCACAACTACACCATACATTGGAACCCTGCTGAAATTGTTTGGTACGTCGATAGTTTGCCCATTCGTGTTTTCCGAAACTACGAGAACCAGGGGATTGCCTACCCCAACAAGCAAGGGATGAAGGTTTACTCCAGCTTATGGAATGCTGATAACTGGGCAACTAGAGGTGGGCTAGAGAAGATAAACTGGACCGGTGCACCCTTCACAGCCGGGTTCAAAAACTTCAGGGCAAGAGCCTGCAAGTGGGATGGAGCCGCCAGCATTAGTGTATGCGCCGCCACTTCCCGGGCTAACTGGTGGACCTCCCCTATATACAGCCGGCTGAGCAGCGGTAAGCTCGGCCAGCTGCAGTGGGTCAGGAATAACCACATGATCTATGACTACTGCAAAGACACTAAACGATTCAATGGAAAGATGCCATCTGAATGTTCTAAACAACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

289

Amino Acids

32.48

Weight (kDa)

8.97

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 28 - 204 1.1e-61 Glycosyl hydrolases family 16
XET_C PF06955 235 - 283 9.7e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28850
fragaria_vesca FvH4_7g07550
malus_domestica MD02G1057800.v1.1 MD15G1192200.v1.1
prunus_persica Prupe.7G225000_v2.0.a1
pyrus_communis pycom02g04690 pycom15g17210
rosa_chinensis RchiOBHm_Chr2g0091321
rosa_laevigata RLG00000016211
rosa_multiflora Rmu_sc0002270.1_g000048
rosa_roxburghii Rroxscaffold_2G00150210
rosa_rugosa Rorug02G0016300
rosa_samantha Rh2CG062600 Rh2DG061200
rosa_wichuraiana Rw2G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 104, 226
AccB1I GGYRCC 2 cut(s) 104, 226
AccB7I CCANNNNNTGG 2 cut(s) 438, 558
AccII CGCG 1 cut(s) 413
AciI CCGC 6 cut(s) 240, 411, 413, 675, 696, 750
AclI AACGTT 1 cut(s) 318
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 3 cut(s) 58, 235, 305
AcuI CTGAAG 2 cut(s) 144, 628
AfaI GTAC 5 cut(s) 92, 106, 228, 260, 464
AfiI CCNNNNNNNGG 6 cut(s) 100, 412, 438, 558, 663, 705
AgeI ACCGGT 1 cut(s) 611
AgsI TTSAA 4 cut(s) 125, 305, 637, 826
AjnI CCWGG 3 cut(s) 98, 229, 507
AluBI AGCT 5 cut(s) 11, 32, 555, 757, 766
AluI AGCT 5 cut(s) 11, 32, 555, 757, 766
Alw21I GWGCWC 2 cut(s) 150, 619
Alw44I GTGCAC 1 cut(s) 615
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 1 cut(s) 760
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 4 cut(s) 170, 382, 747, 766
ApoI RAATTY 3 cut(s) 58, 235, 305
AsiGI ACCGGT 1 cut(s) 611
Asp718I GGTACC 2 cut(s) 104, 226
AspA2I CCTAGG 1 cut(s) 310
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 3 cut(s) 278, 609, 720
AsuC2I CCSGG 4 cut(s) 244, 631, 706, 707
AsuHPI GGTGA 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 3 cut(s) 278, 609, 720
AvrII CCTAGG 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 2 cut(s) 104, 226
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 2 cut(s) 150, 619
BbvI GCAGC 4 cut(s) 157, 394, 753, 759
BccI CCATC 3 cut(s) 60, 662, 847
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 3 cut(s) 100, 231, 509
BcnI CCSGG 4 cut(s) 244, 631, 706, 707
BfaI CTAG 4 cut(s) 8, 311, 582, 593
BfmI CTRYAG 1 cut(s) 767
BisI GCNGC 6 cut(s) 171, 383, 675, 696, 748, 767
BlnI CCTAGG 1 cut(s) 310
BlpI GCTNAGC 1 cut(s) 743
BlsI GCNGC 6 cut(s) 172, 384, 676, 697, 749, 768
Bme1390I CCNGG 7 cut(s) 100, 231, 244, 509, 631, 706, 707
Bme18I GGWCC 3 cut(s) 278, 609, 720
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 3 cut(s) 278, 609, 720
BmiI GGNNCC 6 cut(s) 106, 228, 280, 373, 443, 673
BmrFI CCNGG 7 cut(s) 100, 231, 244, 509, 631, 706, 707
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 5 cut(s) 48, 58, 201, 213, 825
BmuI ACTGGG 1 cut(s) 583
BpmI CTGGAG 1 cut(s) 535
Bpu1102I GCTNAGC 1 cut(s) 743
Bpu14I TTCGAA 1 cut(s) 80
BpuMI CCSGG 4 cut(s) 244, 631, 706, 707
BsaJI CCNNGG 6 cut(s) 99, 230, 310, 411, 508, 705
BsaWI WCCGGW 1 cut(s) 611
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 412, 438, 558, 663, 705
Bse118I RCCGGY 2 cut(s) 611, 738
Bse1I ACTGG 3 cut(s) 578, 611, 719
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 3 cut(s) 100, 231, 509
BseDI CCNNGG 6 cut(s) 99, 230, 310, 411, 508, 705
BseGI GGATG 2 cut(s) 543, 673
BseLI CCNNNNNNNGG 6 cut(s) 100, 412, 438, 558, 663, 705
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 734
BseNI ACTGG 3 cut(s) 578, 611, 719
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 4 cut(s) 157, 394, 753, 759
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 1 cut(s) 762
BshNI GGYRCC 2 cut(s) 104, 226
BshTI ACCGGT 1 cut(s) 611
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 150, 619
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 5 cut(s) 243, 612, 630, 706, 739
BslI CCNNNNNNNGG 6 cut(s) 100, 412, 438, 558, 663, 705
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 705
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 150, 619
Bsp143I GATC 2 cut(s) 151, 792
Bsp1720I GCTNAGC 1 cut(s) 743
BspACI CCGC 6 cut(s) 240, 411, 413, 675, 696, 750
BspANI GGCC 1 cut(s) 762
BspCNI CTCAG 1 cut(s) 735
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 6 cut(s) 106, 228, 280, 373, 443, 673
BspMAI CTGCAG 1 cut(s) 771
BspQI GCTCTTC 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 2 cut(s) 104, 226
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 2 cut(s) 611, 738
BsrI ACTGG 3 cut(s) 578, 611, 719
BssAI RCCGGY 2 cut(s) 611, 738
BssECI CCNNGG 6 cut(s) 99, 230, 310, 411, 508, 705
BssMI GATC 2 cut(s) 151, 792
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 3 cut(s) 100, 231, 509
Bst6I CTCTTC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 5 cut(s) 18, 658, 679, 740, 764
BstDEI CTNAG 1 cut(s) 743
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 2 cut(s) 543, 673
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 2 cut(s) 154, 795
BstMBI GATC 2 cut(s) 151, 792
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 3 cut(s) 17, 744, 763
BstNI CCWGG 3 cut(s) 100, 231, 509
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 7 cut(s) 98, 229, 242, 507, 629, 704, 705
BstSFI CTRYAG 1 cut(s) 767
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 4 cut(s) 157, 394, 753, 759
BsuRI GGCC 1 cut(s) 762
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 2 cut(s) 543, 673
BtsI GCAGTG 2 cut(s) 42, 776
BtsIMutI CAGTG 2 cut(s) 42, 776
Cac8I GCNNGC 5 cut(s) 18, 658, 679, 740, 764
CaiI CAGNNNCTG 1 cut(s) 167
CfoI GCGC 1 cut(s) 695
Cfr10I RCCGGY 2 cut(s) 611, 738
Cfr13I GGNCC 3 cut(s) 278, 609, 720
Cfr42I CCGCGG 1 cut(s) 414
Cfr9I CCCGGG 1 cut(s) 705
Csp6I GTAC 5 cut(s) 91, 105, 227, 259, 463
CspAI ACCGGT 1 cut(s) 611
CviAII CATG 2 cut(s) 88, 790
CviQI GTAC 5 cut(s) 91, 105, 227, 259, 463
DdeI CTNAG 1 cut(s) 743
DpnI GATC 2 cut(s) 153, 794
DpnII GATC 2 cut(s) 151, 792
EaeI YGGCCR 1 cut(s) 760
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
EciI GGCGGA 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 278, 609, 720
Eco57I CTGAAG 2 cut(s) 144, 628
Eco88I CYCGRG 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 278
EcoRI GAATTC 2 cut(s) 235, 305
EcoRII CCWGG 3 cut(s) 98, 229, 507
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 2 cut(s) 91, 793
FatI CATG 2 cut(s) 87, 789
Fnu4HI GCNGC 6 cut(s) 171, 383, 675, 696, 748, 767
FokI GGATG 2 cut(s) 550, 680
Fsp4HI GCNGC 6 cut(s) 171, 383, 675, 696, 748, 767
FspBI CTAG 4 cut(s) 8, 311, 582, 593
GlaI GCGC 1 cut(s) 694
GluI GCNGC 6 cut(s) 171, 383, 675, 696, 748, 767
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 1 cut(s) 762
HapII CCGG 5 cut(s) 243, 612, 630, 706, 739
HhaI GCGC 1 cut(s) 695
Hin1II CATG 2 cut(s) 91, 793
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 3 cut(s) 121, 133, 822
HpaII CCGG 5 cut(s) 243, 612, 630, 706, 739
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 5 cut(s) 273, 343, 547, 617, 720
Hpy188I TCNGA 4 cut(s) 200, 494, 844, 866
Hpy188III TCNNGA 3 cut(s) 208, 324, 778
Hpy8I GTNNAC 5 cut(s) 273, 343, 547, 617, 720
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 535, 631
HpyCH4IV ACGT 2 cut(s) 318, 465
HpyCH4V TGCA 8 cut(s) 16, 39, 71, 173, 617, 660, 769, 807
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 744, 763
HpyF3I CTNAG 1 cut(s) 743
HpySE526I ACGT 2 cut(s) 318, 465
Hsp92II CATG 2 cut(s) 91, 793
HspAI GCGC 1 cut(s) 693
KpnI GGTACC 2 cut(s) 108, 230
KroI GCCGGC 1 cut(s) 738
KroNI GCCGGC 1 cut(s) 740
KspI CCGCGG 1 cut(s) 414
Kzo9I GATC 2 cut(s) 151, 792
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 671
Lsp1109I GCAGC 4 cut(s) 157, 394, 753, 759
LweI GCATC 5 cut(s) 48, 58, 201, 213, 825
MaeI CTAG 4 cut(s) 8, 311, 582, 593
MaeII ACGT 2 cut(s) 318, 465
MaeIII GTNAC 1 cut(s) 250
MalI GATC 2 cut(s) 153, 794
MboI GATC 2 cut(s) 151, 792
MboII GAAGA 2 cut(s) 94, 610
MhlI GDGCHC 2 cut(s) 150, 619
MluCI AATT 6 cut(s) 58, 235, 305, 386, 453, 860
MnlI CCTC 6 cut(s) 104, 152, 179, 404, 578, 733
MroNI GCCGGC 1 cut(s) 738
MseI TTAA 2 cut(s) 138, 219
MslI CAYNNNNRTG 2 cut(s) 209, 844
MspA1I CMGCKG 3 cut(s) 413, 750, 766
MspI CCGG 5 cut(s) 243, 612, 630, 706, 739
MspR9I CCNGG 7 cut(s) 100, 231, 244, 509, 631, 706, 707
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 3 cut(s) 100, 231, 509
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 3 cut(s) 17, 744, 763
NaeI GCCGGC 1 cut(s) 740
NciI CCSGG 4 cut(s) 244, 631, 706, 707
NdeII GATC 2 cut(s) 151, 792
NgoMIV GCCGGC 1 cut(s) 738
NlaIII CATG 2 cut(s) 91, 793
NlaIV GGNNCC 6 cut(s) 106, 228, 280, 373, 443, 673
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 91
NspV TTCGAA 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 2 cut(s) 204, 490
PctI GAATGC 1 cut(s) 568
PdiI GCCGGC 1 cut(s) 740
PfeI GAWTC 3 cut(s) 121, 133, 822
PflMI CCANNNNNTGG 2 cut(s) 438, 558
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 6 cut(s) 172, 384, 676, 697, 749, 768
PpuMI RGGWCCY 1 cut(s) 278
Psp1406I AACGTT 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 3 cut(s) 98, 229, 507
PspGI CCWGG 3 cut(s) 98, 229, 507
PspN4I GGNNCC 6 cut(s) 106, 228, 280, 373, 443, 673
PspPI GGNCC 3 cut(s) 278, 609, 720
PspPPI RGGWCCY 1 cut(s) 278
PstI CTGCAG 1 cut(s) 771
PstNI CAGNNNCTG 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 766
RsaI GTAC 5 cut(s) 92, 106, 228, 260, 464
RsaNI GTAC 5 cut(s) 91, 105, 227, 259, 463
RseI CAYNNNNRTG 2 cut(s) 209, 844
SacII CCGCGG 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 138, 219
SatI GCNGC 6 cut(s) 171, 383, 675, 696, 748, 767
Sau3AI GATC 2 cut(s) 151, 792
Sau96I GGNCC 3 cut(s) 278, 609, 720
ScrFI CCNGG 7 cut(s) 100, 231, 244, 509, 631, 706, 707
SduI GDGCHC 2 cut(s) 150, 619
SfaNI GCATC 5 cut(s) 48, 58, 201, 213, 825
SfcI CTRYAG 1 cut(s) 767
Sfr303I CCGCGG 1 cut(s) 414
SfuI TTCGAA 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 3 cut(s) 278, 609, 720
SmaI CCCGGG 1 cut(s) 707
SmiMI CAYNNNNRTG 2 cut(s) 209, 844
Sse9I AATT 6 cut(s) 58, 235, 305, 386, 453, 860
SsiI CCGC 6 cut(s) 240, 411, 413, 675, 696, 750
SspMI CTAG 4 cut(s) 8, 311, 582, 593
StyD4I CCNGG 7 cut(s) 98, 229, 242, 507, 629, 704, 705
StyI CCWWGG 1 cut(s) 310
TaiI ACGT 2 cut(s) 321, 468
TaqI TCGA 4 cut(s) 80, 150, 207, 468
TasI AATT 6 cut(s) 58, 235, 305, 386, 453, 860
TauI GCSGC 2 cut(s) 677, 698
TfiI GAWTC 3 cut(s) 121, 133, 822
Tru1I TTAA 2 cut(s) 138, 219
Tru9I TTAA 2 cut(s) 138, 219
TscAI CASTG 2 cut(s) 49, 776
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 4 cut(s) 170, 382, 747, 766
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 1 cut(s) 554
TspMI CCCGGG 1 cut(s) 705
TspRI CASTG 2 cut(s) 49, 776
Van91I CCANNNNNTGG 2 cut(s) 438, 558
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 3 cut(s) 278, 609, 720
XapI RAATTY 3 cut(s) 58, 235, 305
XceI RCATGY 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 705
XmaJI CCTAGG 1 cut(s) 310
XspI CTAG 4 cut(s) 8, 311, 582, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.