pycom02g04690

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016167
Physical Location & Seq
Forward (+)
3055081 .. 3056361
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g04690.1

Sequence Viewer

Length: 810 bp
ATGGCAGTTTTGCAAGCATTCCTGGTAGCTCTATGCATCACTGTGTTCCATCAAAATGTAGCTGATGCAACTGCTGCAAAGAGCATGTACCTCACCTGGGGTACCCAACACGCTGTGATTCAAGGCGAGAATCTTAACCTCGTGCTTGATCAAACTTCAGAGAATAAACTAACGTGTGCTAATTTGTTCATACTCGAATTTGTGCAGCTATCATCAACAGGAACCAAACACGACGAGATAGATTTTGAATTCCTAGGGAACGTTTCTGGACAACCTTACATTGTCCACACAAACATATATACACAAGGAAATGGAAGCAAAGAGCAGCAATTTTACCTCTGGTTTGACCCGTCTGCTGATTTCCACAACTACACCATACATTGGAACCCTACTGAAGTTGTGTGGTATGTCGATAGTTTGCCCATTCGTGTTTTCCGAAACTACGAAAACCAGGGGATTGCTTACCCCAACAAGCAAGGGATGCGGGTTTACTCCAGCTTATGGAATGCTGATAACTGGGCAACTAGAGGTGGGCTAGTGAAGATAAATTGGACCAGTGCGCCCTTCACAGCCGGATTCAAACACTTCAGGGCAAGAGCCTGCAAGTGGAATGGAGCTGCCAGCATTAGTGTATGCGCCGCCACTTCCCAGGCTAACTGGTGGACTTCCCCTATATACAGCCAGCTGAGCAACAGTAAGCTCGGCCAGCTGCAATGGGTCAGGAATAACTACATGATCTATGACTACTGCAAAGACACCACACGATTCAATGGAAATACGCCACCTGAATGTTCTAAACAACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

270

Amino Acids

30.68

Weight (kDa)

7.03

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 70 - 185 1.3e-43 Glycosyl hydrolases family 16
XET_C PF06955 216 - 264 5.4e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28850
fragaria_vesca FvH4_7g07550
malus_domestica MD02G1057800.v1.1 MD15G1192200.v1.1
prunus_persica Prupe.7G225000_v2.0.a1
pyrus_communis pycom02g04690 pycom15g17210
rosa_chinensis RchiOBHm_Chr2g0091321
rosa_laevigata RLG00000016211
rosa_multiflora Rmu_sc0002270.1_g000048
rosa_roxburghii Rroxscaffold_2G00150210
rosa_rugosa Rorug02G0016300
rosa_samantha Rh2CG062600 Rh2DG061200
rosa_wichuraiana Rw2G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 2 cut(s) 381, 501
AciI CCGC 2 cut(s) 484, 639
AclI AACGTT 1 cut(s) 261
AcoI YGGCCR 1 cut(s) 703
AcsI RAATTY 2 cut(s) 197, 248
AcuI CTGAAG 3 cut(s) 141, 414, 571
AdeI CACNNNGTG 1 cut(s) 115
AfaI GTAC 2 cut(s) 89, 103
AfiI CCNNNNNNNGG 4 cut(s) 97, 381, 501, 606
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 4 cut(s) 122, 248, 580, 769
AjnI CCWGG 4 cut(s) 21, 95, 450, 648
AluBI AGCT 8 cut(s) 29, 62, 208, 498, 617, 685, 700, 709
AluI AGCT 8 cut(s) 29, 62, 208, 498, 617, 685, 700, 709
AoxI GGCC 1 cut(s) 703
ApeKI GCWGC 5 cut(s) 74, 205, 325, 617, 709
ApoI RAATTY 2 cut(s) 197, 248
Asp718I GGTACC 1 cut(s) 101
AspA2I CCTAGG 1 cut(s) 253
AspLEI GCGC 2 cut(s) 562, 638
AspS9I GGNCC 1 cut(s) 552
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 552
AvrII CCTAGG 1 cut(s) 253
BanI GGYRCC 1 cut(s) 101
BauI CACGAG 1 cut(s) 140
BbvI GCAGC 5 cut(s) 61, 217, 337, 604, 696
BccI CCATC 1 cut(s) 57
BciT130I CCWGG 4 cut(s) 23, 97, 452, 650
BclI TGATCA 1 cut(s) 148
BfaI CTAG 3 cut(s) 254, 525, 536
BisI GCNGC 6 cut(s) 75, 206, 326, 618, 639, 710
BlnI CCTAGG 1 cut(s) 253
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 6 cut(s) 76, 207, 327, 619, 640, 711
Bme1390I CCNGG 4 cut(s) 23, 97, 452, 650
Bme18I GGWCC 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 3 cut(s) 103, 223, 386
BmrFI CCNGG 4 cut(s) 23, 97, 452, 650
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 3 cut(s) 45, 55, 471
BmuI ACTGGG 1 cut(s) 526
BpmI CTGGAG 1 cut(s) 478
Bpu1102I GCTNAGC 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 96, 253, 451, 648
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 381, 501, 606
Bse1I ACTGG 3 cut(s) 521, 555, 662
Bse3DI GCAATG 1 cut(s) 719
BseBI CCWGG 4 cut(s) 23, 97, 452, 650
BseDI CCNNGG 4 cut(s) 96, 253, 451, 648
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 4 cut(s) 97, 381, 501, 606
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 1 cut(s) 677
BseNI ACTGG 3 cut(s) 521, 555, 662
BseXI GCAGC 5 cut(s) 61, 217, 337, 604, 696
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 1 cut(s) 705
BshNI GGYRCC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 573
BslI CCNNNNNNNGG 4 cut(s) 97, 381, 501, 606
BsmI GAATGC 2 cut(s) 17, 511
BsnI GGCC 1 cut(s) 705
Bsp143I GATC 2 cut(s) 148, 735
Bsp1720I GCTNAGC 1 cut(s) 686
BspACI CCGC 2 cut(s) 484, 639
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 103, 223, 386
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 719
BsrI ACTGG 3 cut(s) 521, 555, 662
BssECI CCNNGG 4 cut(s) 96, 253, 451, 648
BssMI GATC 2 cut(s) 148, 735
BssSI CACGAG 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 4 cut(s) 23, 97, 452, 650
Bst4CI ACNGT 2 cut(s) 43, 695
BstAPI GCANNNNNTGC 2 cut(s) 74, 481
BstC8I GCNNGC 5 cut(s) 15, 601, 622, 683, 707
BstDEI CTNAG 1 cut(s) 686
BstF5I GGATG 1 cut(s) 486
BstHHI GCGC 2 cut(s) 562, 638
BstKTI GATC 2 cut(s) 151, 738
BstMBI GATC 2 cut(s) 148, 735
BstMWI GCNNNNNNNGC 4 cut(s) 74, 481, 687, 706
BstNI CCWGG 4 cut(s) 23, 97, 452, 650
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 4 cut(s) 21, 95, 450, 648
BstV1I GCAGC 5 cut(s) 61, 217, 337, 604, 696
BsuRI GGCC 1 cut(s) 705
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 39, 562
Cac8I GCNNGC 5 cut(s) 15, 601, 622, 683, 707
CfoI GCGC 2 cut(s) 562, 638
Cfr13I GGNCC 1 cut(s) 552
Csp6I GTAC 2 cut(s) 88, 102
CviAII CATG 2 cut(s) 85, 733
CviQI GTAC 2 cut(s) 88, 102
DdeI CTNAG 1 cut(s) 686
DpnI GATC 2 cut(s) 150, 737
DpnII GATC 2 cut(s) 148, 735
DraIII CACNNNGTG 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 552
Eco57I CTGAAG 3 cut(s) 141, 414, 571
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 4 cut(s) 21, 95, 450, 648
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 2 cut(s) 88, 736
FatI CATG 2 cut(s) 84, 732
FauI CCCGC 1 cut(s) 477
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 6 cut(s) 75, 206, 326, 618, 639, 710
FokI GGATG 1 cut(s) 493
Fsp4HI GCNGC 6 cut(s) 75, 206, 326, 618, 639, 710
FspBI CTAG 3 cut(s) 254, 525, 536
GlaI GCGC 2 cut(s) 561, 637
GluI GCNGC 6 cut(s) 75, 206, 326, 618, 639, 710
GsuI CTGGAG 1 cut(s) 478
HaeIII GGCC 1 cut(s) 705
HapII CCGG 1 cut(s) 573
HhaI GCGC 2 cut(s) 562, 638
Hin1II CATG 2 cut(s) 88, 736
Hin6I GCGC 2 cut(s) 560, 636
HinP1I GCGC 2 cut(s) 560, 636
HinfI GANTC 4 cut(s) 118, 130, 576, 765
HpaII CCGG 1 cut(s) 573
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 3 cut(s) 286, 490, 663
Hpy188I TCNGA 3 cut(s) 160, 437, 809
Hpy188III TCNNGA 2 cut(s) 267, 721
Hpy8I GTNNAC 3 cut(s) 286, 490, 663
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 574
HpyCH4III ACNGT 2 cut(s) 43, 695
HpyCH4IV ACGT 2 cut(s) 173, 261
HpyCH4V TGCA 8 cut(s) 13, 36, 68, 77, 205, 603, 712, 750
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 481, 687, 706
HpyF3I CTNAG 1 cut(s) 686
HpySE526I ACGT 2 cut(s) 173, 261
Hsp92II CATG 2 cut(s) 88, 736
HspAI GCGC 2 cut(s) 560, 636
KpnI GGTACC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 2 cut(s) 148, 735
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 5 cut(s) 61, 217, 337, 604, 696
LweI GCATC 3 cut(s) 45, 55, 471
MaeI CTAG 3 cut(s) 254, 525, 536
MaeII ACGT 2 cut(s) 173, 261
MalI GATC 2 cut(s) 150, 737
MboI GATC 2 cut(s) 148, 735
MboII GAAGA 1 cut(s) 553
MluCI AATT 6 cut(s) 181, 197, 248, 329, 547, 803
MnlI CCTC 4 cut(s) 101, 149, 347, 521
Mph1103I ATGCAT 1 cut(s) 38
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 3 cut(s) 41, 54, 787
MspA1I CMGCKG 2 cut(s) 685, 709
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 4 cut(s) 23, 97, 452, 650
Mva1269I GAATGC 2 cut(s) 17, 511
MvaI CCWGG 4 cut(s) 23, 97, 452, 650
MwoI GCNNNNNNNGC 4 cut(s) 74, 481, 687, 706
NdeII GATC 2 cut(s) 148, 735
NlaIII CATG 2 cut(s) 88, 736
NlaIV GGNNCC 3 cut(s) 103, 223, 386
NmeAIII GCCGAG 1 cut(s) 681
NsiI ATGCAT 1 cut(s) 38
NspI RCATGY 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PctI GAATGC 2 cut(s) 17, 511
PfeI GAWTC 4 cut(s) 118, 130, 576, 765
PflMI CCANNNNNTGG 2 cut(s) 381, 501
PkrI GCNGC 6 cut(s) 76, 207, 327, 619, 640, 711
Psp1406I AACGTT 1 cut(s) 261
Psp6I CCWGG 4 cut(s) 21, 95, 450, 648
PspGI CCWGG 4 cut(s) 21, 95, 450, 648
PspN4I GGNNCC 3 cut(s) 103, 223, 386
PspPI GGNCC 1 cut(s) 552
PvuII CAGCTG 2 cut(s) 685, 709
RsaI GTAC 2 cut(s) 89, 103
RsaNI GTAC 2 cut(s) 88, 102
RseI CAYNNNNRTG 3 cut(s) 41, 54, 787
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 6 cut(s) 75, 206, 326, 618, 639, 710
Sau3AI GATC 2 cut(s) 148, 735
Sau96I GGNCC 1 cut(s) 552
ScrFI CCNGG 4 cut(s) 23, 97, 452, 650
SfaNI GCATC 3 cut(s) 45, 55, 471
SinI GGWCC 1 cut(s) 552
SmiMI CAYNNNNRTG 3 cut(s) 41, 54, 787
Sse9I AATT 6 cut(s) 181, 197, 248, 329, 547, 803
SsiI CCGC 2 cut(s) 484, 639
SspMI CTAG 3 cut(s) 254, 525, 536
StyD4I CCNGG 4 cut(s) 21, 95, 450, 648
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 43, 695
TaiI ACGT 2 cut(s) 176, 264
TaqI TCGA 2 cut(s) 195, 411
TasI AATT 6 cut(s) 181, 197, 248, 329, 547, 803
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 4 cut(s) 118, 130, 576, 765
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 46, 562
TseI GCWGC 5 cut(s) 74, 205, 325, 617, 709
TspDTI ATGAA 1 cut(s) 178
TspRI CASTG 2 cut(s) 46, 562
Van91I CCANNNNNTGG 2 cut(s) 381, 501
VpaK11BI GGWCC 1 cut(s) 552
XapI RAATTY 2 cut(s) 197, 248
XceI RCATGY 1 cut(s) 88
XmaJI CCTAGG 1 cut(s) 253
XspI CTAG 3 cut(s) 254, 525, 536
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.