FvH4_7g07550

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016167
Physical Location & Seq
Forward (+)
7439553 .. 7441357
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07550.t1

Sequence Viewer

Length: 870 bp
ATGGCGATTCTGCAAGCTTTCTTGATAGGCTTGTGCACCATTGCTTTTTATCAAAGTTCAGTTAATGCAAAGTTTTCGAAGAGCATGTACATCACCTGGGGTCAGCAGCATGCTGCAATTCAAGGCAATGGCGAAGATGTCCAACTCGTGCTCGATCAAACTTCAGGATCTGCTGTTCAATCAAAGAGAGGTTTCTTATTCGGAAGCATTGAGATGCTCATTAAGTTGGTACCTAATAATTCTGCAGGCACAGTAACAGCTTTCTATCTCTCATCTACAGGAAGCAAACATGACGAGATAGATTTCGAATTCTTAGGAAACGTTTCTGGACAACCTTACATTATCCACACAAACATCTATACACAAGGAAATGGAAGCAGAGAGCAGCAGTTTTACCTCTGGTTTGATCCAACAGCTGATTTTCACAACTACACCATACATTGGAACCCAACCACAGTTGTGTGGTACATCGATGGACTGCCAATTCGTGTATTCAGAAACTACGAAAACGAGGGCATTGCTTACCCCAGCAAGCAAGGGATGAGGGTTTACTCCAGCTTATGGAACGCAGATAACTGGGCAACCAGAGGCGGACTAGTCAAAATCGACTGGACCAGCGCCCCATTCATAGCTAGATTGCGCAATTTCAGAGCGAGAGCTTGCAAGTGGGATGGACCGAGCAGTATCACGCAATGCGCCGCAGCTACTCCTGCAAACTGGTGGACATCTCCTATATACAAGCAGCTGAGCAATGCTAAGTTAGGCCAGCTGAAATGGGTTAGAGACAACCACATGATCTACGACTACTGCAAAGACACTAAACGATTCAACGGAAACATGCCTGTGGAATGCTCCAAACAACAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

290

Amino Acids

32.84

Weight (kDa)

8.86

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 26 - 205 1.1e-63 Glycosyl hydrolases family 16
XET_C PF06955 236 - 284 6.5e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28850
fragaria_vesca FvH4_7g07550
malus_domestica MD02G1057800.v1.1 MD15G1192200.v1.1
prunus_persica Prupe.7G225000_v2.0.a1
pyrus_communis pycom02g04690 pycom15g17210
rosa_chinensis RchiOBHm_Chr2g0091321
rosa_laevigata RLG00000016211
rosa_multiflora Rmu_sc0002270.1_g000048
rosa_roxburghii Rroxscaffold_2G00150210
rosa_rugosa Rorug02G0016300
rosa_samantha Rh2CG062600 Rh2DG061200
rosa_wichuraiana Rw2G004840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 641
Acc65I GGTACC 1 cut(s) 229
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 2 cut(s) 441, 561
AciI CCGC 2 cut(s) 591, 699
AclI AACGTT 1 cut(s) 321
AclWI GGATC 2 cut(s) 175, 401
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 3 cut(s) 89, 231, 467
AfiI CCNNNNNNNGG 2 cut(s) 441, 561
AgsI TTSAA 3 cut(s) 122, 179, 829
AhlI ACTAGT 1 cut(s) 595
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 1 cut(s) 848
AleI CACNNNNGTG 1 cut(s) 458
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 9 cut(s) 17, 260, 416, 558, 632, 659, 704, 745, 769
AluI AGCT 9 cut(s) 17, 260, 416, 558, 632, 659, 704, 745, 769
Alw21I GWGCWC 2 cut(s) 38, 153
Alw26I GTCTC 1 cut(s) 777
Alw44I GTGCAC 1 cut(s) 34
AlwI GGATC 2 cut(s) 175, 401
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 1 cut(s) 763
ApaLI GTGCAC 1 cut(s) 34
ApeKI GCWGC 5 cut(s) 106, 113, 385, 701, 742
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 468, 500
Asp700I GAANNNNTTC 1 cut(s) 322
Asp718I GGTACC 1 cut(s) 229
AspLEI GCGC 3 cut(s) 620, 642, 698
AspS9I GGNCC 2 cut(s) 612, 674
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 2 cut(s) 77, 306
AvaII GGWCC 2 cut(s) 612, 674
BaeGI GKGCMC 1 cut(s) 38
BanI GGYRCC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 38, 153
BbvI GCAGC 5 cut(s) 100, 118, 397, 713, 754
BccI CCATC 2 cut(s) 467, 665
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 500, 534
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 777
BcuI ACTAGT 1 cut(s) 595
BfaI CTAG 2 cut(s) 596, 633
BfmI CTRYAG 2 cut(s) 243, 276
BfoI RGCGCY 1 cut(s) 621
BisI GCNGC 6 cut(s) 107, 114, 386, 699, 702, 743
BlpI GCTNAGC 1 cut(s) 746
BlsI GCNGC 6 cut(s) 108, 115, 387, 700, 703, 744
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 2 cut(s) 612, 674
BmgT120I GGNCC 2 cut(s) 612, 674
BmiI GGNNCC 2 cut(s) 231, 446
BmrFI CCNGG 1 cut(s) 97
BmrI ACTGGG 1 cut(s) 586
BmsI GCATC 1 cut(s) 204
BmuI ACTGGG 1 cut(s) 586
BpmI CTGGAG 1 cut(s) 538
Bpu1102I GCTNAGC 1 cut(s) 746
Bpu14I TTCGAA 2 cut(s) 77, 306
Bsa29I ATCGAT 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 561
Bse1I ACTGG 3 cut(s) 581, 614, 722
Bse3DI GCAATG 5 cut(s) 39, 133, 516, 698, 757
BseBI CCWGG 1 cut(s) 97
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 2 cut(s) 546, 676
BseLI CCNNNNNNNGG 2 cut(s) 441, 561
BseMI GCAATG 5 cut(s) 39, 133, 516, 698, 757
BseMII CTCAG 1 cut(s) 737
BseNI ACTGG 3 cut(s) 581, 614, 722
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 5 cut(s) 100, 118, 397, 713, 754
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 765
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 38, 153
BslI CCNNNNNNNGG 2 cut(s) 441, 561
BsmAI GTCTC 1 cut(s) 777
BsmI GAATGC 1 cut(s) 854
BsnI GGCC 1 cut(s) 765
Bsp119I TTCGAA 2 cut(s) 77, 306
Bsp1286I GDGCHC 2 cut(s) 38, 153
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 4 cut(s) 154, 167, 406, 795
Bsp1720I GCTNAGC 1 cut(s) 746
BspACI CCGC 2 cut(s) 591, 699
BspANI GGCC 1 cut(s) 765
BspCNI CTCAG 1 cut(s) 738
BspDI ATCGAT 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 231, 446
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 2 cut(s) 175, 401
BspQI GCTCTTC 1 cut(s) 74
BspT104I TTCGAA 2 cut(s) 77, 306
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 5 cut(s) 39, 133, 516, 698, 757
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 3 cut(s) 581, 614, 722
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 4 cut(s) 154, 167, 406, 795
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 253, 457
Bst6I CTCTTC 1 cut(s) 74
BstAUI TGTACA 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 77, 306
BstC8I GCNNGC 6 cut(s) 15, 111, 247, 533, 661, 767
BstDEI CTNAG 3 cut(s) 313, 746, 756
BstF5I GGATG 2 cut(s) 546, 676
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 620, 642, 698
BstKTI GATC 4 cut(s) 157, 170, 409, 798
BstMAI GTCTC 1 cut(s) 777
BstMBI GATC 4 cut(s) 154, 167, 406, 795
BstMWI GCNNNNNNNGC 1 cut(s) 710
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 3 cut(s) 88, 113, 841
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 243, 276
BstSLI GKGCMC 1 cut(s) 38
BstV1I GCAGC 5 cut(s) 100, 118, 397, 713, 754
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 1 cut(s) 765
BsuTUI ATCGAT 1 cut(s) 471
BtsCI GGATG 2 cut(s) 546, 676
Cac8I GCNNGC 6 cut(s) 15, 111, 247, 533, 661, 767
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 3 cut(s) 620, 642, 698
Cfr13I GGNCC 2 cut(s) 612, 674
ClaI ATCGAT 1 cut(s) 471
Csp6I GTAC 3 cut(s) 88, 230, 466
CviAII CATG 5 cut(s) 85, 110, 290, 793, 838
CviQI GTAC 3 cut(s) 88, 230, 466
DdeI CTNAG 3 cut(s) 313, 746, 756
DpnI GATC 4 cut(s) 156, 169, 408, 797
DpnII GATC 4 cut(s) 154, 167, 406, 795
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EciI GGCGGA 1 cut(s) 606
Eco47I GGWCC 2 cut(s) 612, 674
Eco57I CTGAAG 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 95
FaeI CATG 5 cut(s) 88, 113, 293, 796, 841
FatI CATG 5 cut(s) 84, 109, 289, 792, 837
Fnu4HI GCNGC 6 cut(s) 107, 114, 386, 699, 702, 743
FokI GGATG 2 cut(s) 553, 683
Fsp4HI GCNGC 6 cut(s) 107, 114, 386, 699, 702, 743
FspBI CTAG 2 cut(s) 596, 633
FspI TGCGCA 1 cut(s) 641
GlaI GCGC 3 cut(s) 619, 641, 697
GluI GCNGC 6 cut(s) 107, 114, 386, 699, 702, 743
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 538
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 1 cut(s) 765
HhaI GCGC 3 cut(s) 620, 642, 698
Hin1II CATG 5 cut(s) 88, 113, 293, 796, 841
Hin6I GCGC 3 cut(s) 618, 640, 696
HinP1I GCGC 3 cut(s) 618, 640, 696
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 7, 825
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 3 cut(s) 36, 550, 723
Hpy188I TCNGA 3 cut(s) 203, 497, 650
Hpy188III TCNNGA 3 cut(s) 22, 165, 327
Hpy8I GTNNAC 3 cut(s) 36, 550, 723
HpyCH4III ACNGT 2 cut(s) 253, 457
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 8 cut(s) 13, 36, 68, 116, 245, 663, 713, 810
HpyF10VI GCNNNNNNNGC 1 cut(s) 710
HpyF3I CTNAG 3 cut(s) 313, 746, 756
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 5 cut(s) 88, 113, 293, 796, 841
HspAI GCGC 3 cut(s) 618, 640, 696
KpnI GGTACC 1 cut(s) 233
Kzo9I GATC 4 cut(s) 154, 167, 406, 795
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 1 cut(s) 857
Lsp1109I GCAGC 5 cut(s) 100, 118, 397, 713, 754
LweI GCATC 1 cut(s) 204
MaeI CTAG 2 cut(s) 596, 633
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 253
MalI GATC 4 cut(s) 156, 169, 408, 797
MboI GATC 4 cut(s) 154, 167, 406, 795
MboII GAAGA 2 cut(s) 91, 146
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 2 cut(s) 38, 153
MluCI AATT 6 cut(s) 117, 238, 308, 483, 643, 863
MmeI TCCRAC 2 cut(s) 166, 434
MnlI CCTC 5 cut(s) 182, 407, 505, 537, 581
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 2 cut(s) 63, 222
MslI CAYNNNNRTG 3 cut(s) 212, 458, 842
MspA1I CMGCKG 3 cut(s) 416, 745, 769
MspR9I CCNGG 1 cut(s) 97
Mva1269I GAATGC 1 cut(s) 854
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 1 cut(s) 710
NdeII GATC 4 cut(s) 154, 167, 406, 795
NlaIII CATG 5 cut(s) 88, 113, 293, 796, 841
NlaIV GGNNCC 2 cut(s) 231, 446
NsbI TGCGCA 1 cut(s) 641
NspI RCATGY 3 cut(s) 88, 113, 841
NspV TTCGAA 2 cut(s) 77, 306
OliI CACNNNNGTG 1 cut(s) 458
PaeI GCATGC 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 74
PctI GAATGC 1 cut(s) 854
PdmI GAANNNNTTC 1 cut(s) 322
PfeI GAWTC 2 cut(s) 7, 825
PflMI CCANNNNNTGG 2 cut(s) 441, 561
PkrI GCNGC 6 cut(s) 108, 115, 387, 700, 703, 744
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 231, 446
PspPI GGNCC 2 cut(s) 612, 674
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 167
PvuII CAGCTG 3 cut(s) 416, 745, 769
RsaI GTAC 3 cut(s) 89, 231, 467
RsaNI GTAC 3 cut(s) 88, 230, 466
RseI CAYNNNNRTG 3 cut(s) 212, 458, 842
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 63, 222
SatI GCNGC 6 cut(s) 107, 114, 386, 699, 702, 743
Sau3AI GATC 4 cut(s) 154, 167, 406, 795
Sau96I GGNCC 2 cut(s) 612, 674
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 2 cut(s) 38, 153
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 2 cut(s) 243, 276
SfuI TTCGAA 2 cut(s) 77, 306
SinI GGWCC 2 cut(s) 612, 674
SmiMI CAYNNNNRTG 3 cut(s) 212, 458, 842
SpeI ACTAGT 1 cut(s) 595
SphI GCATGC 1 cut(s) 113
Sse9I AATT 6 cut(s) 117, 238, 308, 483, 643, 863
SsiI CCGC 2 cut(s) 591, 699
SspMI CTAG 2 cut(s) 596, 633
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 2 cut(s) 253, 457
TaiI ACGT 1 cut(s) 324
TaqI TCGA 5 cut(s) 77, 153, 306, 471, 606
TaqII GACCGA 1 cut(s) 691
TasI AATT 6 cut(s) 117, 238, 308, 483, 643, 863
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 2 cut(s) 7, 825
Tru1I TTAA 2 cut(s) 63, 222
Tru9I TTAA 2 cut(s) 63, 222
TseI GCWGC 5 cut(s) 106, 113, 385, 701, 742
TspDTI ATGAA 1 cut(s) 616
TspGWI ACGGA 1 cut(s) 846
Van91I CCANNNNNTGG 2 cut(s) 441, 561
VneI GTGCAC 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 612, 674
XapI RAATTY 1 cut(s) 308
XceI RCATGY 3 cut(s) 88, 113, 841
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 2 cut(s) 596, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.