AT4G29720

polyamine oxidase 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012410
Physical Location & Seq
Reverse (-)
14553211 .. 14555276
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29720.1

Sequence Viewer

Length: 1602 bp
ATGGCGAAGAAAGCAAGAATTGTTATAATCGGAGCTGGAATGGCTGGTCTCACGGCGGCGAACAAGCTCTACACAAGCTCCAACAACACCTTCGAGCTCTCAGTCGTCGAAGGCGGTTCTAGAATCGGCGGTAGGATCAATACCTCTGAGTTCTCATCAGAGAAGATTGAGATGGGTGCCACGTGGATCCACGGAATCGGTGGAAGCCCTGTTTATAGAATCGCTAAAGAGACTGGTTCTTTAGTCTCTGATGAGCCATGGGAGTGTATGGATTCCACCATTGATAAAGCTAAGACCTTTGCTGAAGGTGGGTTCGAGATTGAGCCTTCCATTGTTGAATCCATCTCTGGTTTGTTCACTGCTCTCATGGAATTAGCTCAGGGGAAAGAGATCTCTCAATCCGACGCCGATTTGAGTCGTTTGGCTCATATTTACGAAACTGCCACTAGGGTTTGCTCTAAGGGAAGTAGTACTAGTGTTGGGTCGTTTTTGAAATCTGGGTTTGATGCTTATTGGGATTCAATCAGCAATGGAGGAGAAGAAGGAGTTAAAGGGTATGGGAAATGGAGTAGGAAGTCACTTGAAGAAGCCATTTTTACGATGTTTAGTAACACACAGAGGACTTACACATCTGCTGATGAACTCTCGACGCTTGATTTCGCGGCGGAGAGTGAGTATCAGATGTTTCCAGGAGAAGAAATCACTATAGCTAAAGGCTATCTTAGTGTTATTCATCATTTGGCATCTGTGCTTCCTCAAGGTGTTATCCAATTGAATCGAAAGGTCACGAAGATCGAGTGGCAGAGTAATGAAGTGAAGCTGCATTTCTCAGATGGGTCTGTTGTTTTTGCAGATCATGTTATTGTTACTGTCTCTTTAGGTGTGCTTAAAGCAGGGATTGAGACTGATGCTGAATTGTTTAGTCCTCCTTTGCCTGATTTCAAATCAGACGCTATTAGAAGACTAGGCTATGGAGTTGTCAACAAGCTGTTCGTCGAGATGTCTCAAAGAAAGTTCCCCTCTTTGCAGCTTGTGTTTGACCGGGAGGATTCCGAGTTTAGGTTCGTGAAAATTCCATGGTGGATGAGAAGAACCGCGACCATTACCCCAATCCATAGCAATTCAAAGGTCTTGCTTTCTTGGTTTGCAGGCAAAGAAGCTCTCGAGCTTGAGAAACTTACCGATGAGGAGATCAAAGACGCTGTCATGACCACTATCTCTTGCTTGACAGGCAAGGAAGTTAAGAATGATACCGCAAAGCCCTTGACCAATGGCTCATTGAATGATGATGATGAAGCCATGAAGATTACAAAGGTCTTGAAGAGCAAATGGGGAAGTGATCCTCTGTTCAGAGGCTCCTATTCGTATGTAGCGGTTGGATCAAGCGGGGATGACCTAGACGCAATGGCTGAGCCATTGCCAAAGATTAATAAGAAGGTTGGTCAGGTCAATGGTCATGATCAAGCCAAGGTTCATGAGCTTCAAGTCATGTTTGCAGGGGAAGCAACACATAGAACCCATTACTCCACAACTCATGGTGCCTACTATAGTGGTTTAAGGGAAGCCAATAGGCTTCTCAAGCATTACAAATGTAATTTTTGA

Protein Analysis

533

Amino Acids

58.69

Weight (kDa)

5.8

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 9 - 77 1.8e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 14 - 526 6.1e-80 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AccB1I GGYRCC 2 cut(s) 176, 1538
AccII CGCG 2 cut(s) 662, 1097
AciI CCGC 9 cut(s) 56, 114, 129, 662, 665, 1095, 1254, 1373, 1386
AclWI GGATC 5 cut(s) 143, 181, 194, 1334, 1387
AcsI RAATTY 1 cut(s) 1071
AcuI CTGAAG 1 cut(s) 324
AcvI CACGTG 1 cut(s) 183
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 1059
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 6 cut(s) 53, 224, 250, 877, 896, 1008
AlwI GGATC 5 cut(s) 143, 181, 194, 1334, 1387
Ama87I CYCGRG 1 cut(s) 1163
ApeKI GCWGC 2 cut(s) 820, 1027
ApoI RAATTY 1 cut(s) 1071
ArsI GACNNNNNNTTYG 2 cut(s) 1188, 1220
AseI ATTAAT 1 cut(s) 1428
AsuC2I CCSGG 1 cut(s) 1043
AvaI CYCGRG 1 cut(s) 1163
BamHI GGATCC 1 cut(s) 186
BanI GGYRCC 2 cut(s) 176, 1538
BanII GRGCYC 1 cut(s) 99
BbrPI CACGTG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 967
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 807, 1039
BccI CCATC 3 cut(s) 166, 350, 827
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 1 cut(s) 690
BclI TGATCA 1 cut(s) 1459
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 6 cut(s) 53, 224, 250, 877, 896, 1008
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 5 cut(s) 120, 447, 474, 965, 1397
BfmI CTRYAG 2 cut(s) 705, 1546
BglII AGATCT 1 cut(s) 390
BisI GCNGC 4 cut(s) 57, 663, 821, 1028
BlpI GCTNAGC 1 cut(s) 1410
BlsI GCNGC 4 cut(s) 58, 664, 822, 1029
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 2 cut(s) 690, 1043
BmeT110I CYCGRG 1 cut(s) 1163
BmiI GGNNCC 4 cut(s) 178, 188, 1357, 1540
BmrFI CCNGG 2 cut(s) 690, 1043
BmsI GCATC 3 cut(s) 496, 752, 898
BpiI GAAGAC 1 cut(s) 967
Bpu10I CCTNAGC 1 cut(s) 378
Bpu1102I GCTNAGC 1 cut(s) 1410
BpuEI CTTGAG 3 cut(s) 741, 1190, 1562
BpuMI CCSGG 1 cut(s) 1043
BsaAI YACGTR 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 4 cut(s) 190, 257, 1076, 1467
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 659, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 1059
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 3 cut(s) 535, 1410, 1415
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 4 cut(s) 190, 257, 1076, 1467
BseGI GGATG 2 cut(s) 1089, 1396
BseLI CCNNNNNNNGG 1 cut(s) 1059
BseMI GCAATG 3 cut(s) 535, 1410, 1415
BseMII CTCAG 5 cut(s) 114, 138, 392, 843, 1401
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 549, 1202
BseXI GCAGC 2 cut(s) 807, 1039
Bsh1236I CGCG 2 cut(s) 662, 1097
BshNI GGYRCC 2 cut(s) 176, 1538
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 1 cut(s) 1042
BslI CCNNNNNNNGG 1 cut(s) 1059
BsmAI GTCTC 6 cut(s) 53, 224, 250, 877, 896, 1008
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 1163
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
Bsp1720I GCTNAGC 1 cut(s) 1410
Bsp19I CCATGG 2 cut(s) 257, 1076
BspACI CCGC 9 cut(s) 56, 114, 129, 662, 665, 1095, 1254, 1373, 1386
BspCNI CTCAG 5 cut(s) 113, 139, 391, 842, 1402
BspFNI CGCG 2 cut(s) 662, 1097
BspHI TCATGA 3 cut(s) 1206, 1456, 1474
BspLI GGNNCC 4 cut(s) 178, 188, 1357, 1540
BspPI GGATC 5 cut(s) 143, 181, 194, 1334, 1387
BspQI GCTCTTC 1 cut(s) 1316
BspT107I GGYRCC 2 cut(s) 176, 1538
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 3 cut(s) 535, 1410, 1415
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 4 cut(s) 190, 257, 1076, 1467
BssMI GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 3 cut(s) 257, 1076, 1467
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 1 cut(s) 871
Bst6I CTCTTC 1 cut(s) 1316
BstACI GRCGYC 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 1150
BstDEI CTNAG 8 cut(s) 100, 147, 291, 378, 459, 722, 829, 1410
BstDSI CCRYGG 3 cut(s) 190, 257, 1076
BstF5I GGATG 2 cut(s) 1089, 1396
BstFNI CGCG 2 cut(s) 662, 1097
BstKTI GATC 9 cut(s) 138, 189, 393, 795, 856, 1194, 1342, 1382, 1462
BstMAI GTCTC 6 cut(s) 53, 224, 250, 877, 896, 1008
BstMBI GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
BstMWI GCNNNNNNNGC 5 cut(s) 11, 41, 1230, 1502, 1579
BstNI CCWGG 1 cut(s) 690
BstSCI CCNGG 2 cut(s) 688, 1041
BstSFI CTRYAG 2 cut(s) 705, 1546
BstUI CGCG 2 cut(s) 662, 1097
BstV1I GCAGC 2 cut(s) 807, 1039
BstV2I GAAGAC 1 cut(s) 967
BstX2I RGATCY 2 cut(s) 186, 390
BstYI RGATCY 2 cut(s) 186, 390
BtgI CCRYGG 3 cut(s) 190, 257, 1076
BtsCI GGATG 2 cut(s) 1089, 1396
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 1150
CciI TCATGA 3 cut(s) 1206, 1456, 1474
CseI GACGC 5 cut(s) 413, 658, 959, 1208, 1409
Csp6I GTAC 1 cut(s) 471
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 8 cut(s) 100, 147, 291, 378, 459, 722, 829, 1410
DpnI GATC 9 cut(s) 137, 188, 392, 794, 855, 1193, 1341, 1381, 1461
DpnII GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
Eam1104I CTCTTC 1 cut(s) 1316
EarI CTCTTC 1 cut(s) 1316
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 3 cut(s) 257, 1076, 1467
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 324
Eco72I CACGTG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 1163
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 688
EcoT14I CCWWGG 3 cut(s) 257, 1076, 1467
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 3 cut(s) 257, 1076, 1467
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FauI CCCGC 1 cut(s) 1379
FbaI TGATCA 1 cut(s) 1459
Fnu4HI GCNGC 4 cut(s) 57, 663, 821, 1028
FokI GGATG 2 cut(s) 1096, 1403
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 57, 663, 821, 1028
FspBI CTAG 5 cut(s) 120, 447, 474, 965, 1397
GluI GCNGC 4 cut(s) 57, 663, 821, 1028
HapII CCGG 1 cut(s) 1042
HgaI GACGC 5 cut(s) 413, 658, 959, 1208, 1409
Hin1I GRCGYC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 982
HindII GTYRAC 1 cut(s) 982
HinfI GANTC 9 cut(s) 123, 195, 219, 272, 338, 415, 518, 775, 1049
HpaII CCGG 1 cut(s) 1042
Hpy166II GTNNAC 2 cut(s) 357, 982
Hpy8I GTNNAC 2 cut(s) 357, 982
Hpy99I CGWCG 4 cut(s) 110, 407, 652, 998
HpyAV CCTTC 6 cut(s) 100, 104, 299, 336, 536, 1429
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 5 cut(s) 823, 851, 1027, 1148, 1496
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 41, 1230, 1502, 1579
HpyF3I CTNAG 8 cut(s) 100, 147, 291, 378, 459, 722, 829, 1410
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 405
Ksp22I TGATCA 1 cut(s) 1459
Kzo9I GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
LguI GCTCTTC 1 cut(s) 1316
LmnI GCTCC 3 cut(s) 32, 83, 1361
Lsp1109I GCAGC 2 cut(s) 807, 1039
LweI GCATC 3 cut(s) 496, 752, 898
MaeI CTAG 5 cut(s) 120, 447, 474, 965, 1397
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 4 cut(s) 576, 608, 784, 865
MalI GATC 9 cut(s) 137, 188, 392, 794, 855, 1193, 1341, 1381, 1461
MboI GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
MfeI CAATTG 1 cut(s) 770
MflI RGATCY 2 cut(s) 186, 390
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 7 cut(s) 18, 371, 770, 914, 1071, 1120, 1594
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 3 cut(s) 105, 426, 1357
MseI TTAA 5 cut(s) 549, 888, 1242, 1428, 1556
MslI CAYNNNNRTG 1 cut(s) 262
MspI CCGG 1 cut(s) 1042
MspR9I CCNGG 2 cut(s) 690, 1043
MunI CAATTG 1 cut(s) 770
MvaI CCWGG 1 cut(s) 690
MvnI CGCG 2 cut(s) 662, 1097
MwoI GCNNNNNNNGC 5 cut(s) 11, 41, 1230, 1502, 1579
NciI CCSGG 1 cut(s) 1043
NcoI CCATGG 2 cut(s) 257, 1076
NdeII GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
NlaIV GGNNCC 4 cut(s) 178, 188, 1357, 1540
NmuCI GTSAC 2 cut(s) 576, 784
PaeR7I CTCGAG 1 cut(s) 1163
PagI TCATGA 3 cut(s) 1206, 1456, 1474
PciSI GCTCTTC 1 cut(s) 1316
PfeI GAWTC 8 cut(s) 123, 195, 219, 272, 338, 518, 775, 1049
PflFI GACNNNGTC 1 cut(s) 1202
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 4 cut(s) 58, 664, 822, 1029
PleI GAGTC 1 cut(s) 423
PmaCI CACGTG 1 cut(s) 183
PmlI CACGTG 1 cut(s) 183
PpsI GAGTC 1 cut(s) 423
Ppu21I YACGTR 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 1428
PsiI TTATAA 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 688
PspCI CACGTG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 4 cut(s) 178, 188, 1357, 1540
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PsuI RGATCY 2 cut(s) 186, 390
PsyI GACNNNGTC 1 cut(s) 1202
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 262
SacI GAGCTC 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 1316
SaqAI TTAA 5 cut(s) 549, 888, 1242, 1428, 1556
SatI GCNGC 4 cut(s) 57, 663, 821, 1028
Sau3AI GATC 9 cut(s) 135, 186, 390, 792, 853, 1191, 1339, 1379, 1459
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 690, 1043
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 496, 752, 898
SfcI CTRYAG 2 cut(s) 705, 1546
Sfr274I CTCGAG 1 cut(s) 1163
SlaI CTCGAG 1 cut(s) 1163
SmiMI CAYNNNNRTG 1 cut(s) 262
SmlI CTYRAG 4 cut(s) 756, 1163, 1169, 1577
SmoI CTYRAG 4 cut(s) 756, 1163, 1169, 1577
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 7 cut(s) 18, 371, 770, 914, 1071, 1120, 1594
SsiI CCGC 9 cut(s) 56, 114, 129, 662, 665, 1095, 1254, 1373, 1386
SspMI CTAG 5 cut(s) 120, 447, 474, 965, 1397
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 688, 1041
StyI CCWWGG 3 cut(s) 257, 1076, 1467
TaaI ACNGT 1 cut(s) 871
TaiI ACGT 1 cut(s) 185
TaqI TCGA 8 cut(s) 93, 108, 315, 647, 778, 795, 996, 1164
TasI AATT 7 cut(s) 18, 371, 770, 914, 1071, 1120, 1594
TatI WGTACW 1 cut(s) 470
TauI GCSGC 2 cut(s) 59, 665
TfiI GAWTC 8 cut(s) 123, 195, 219, 272, 338, 518, 775, 1049
Tru1I TTAA 5 cut(s) 549, 888, 1242, 1428, 1556
Tru9I TTAA 5 cut(s) 549, 888, 1242, 1428, 1556
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 576, 784
TseI GCWGC 2 cut(s) 820, 1027
Tsp45I GTSAC 2 cut(s) 576, 784
TspDTI ATGAA 6 cut(s) 654, 722, 825, 1308, 1316, 1463
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 1 cut(s) 364
Tth111I GACNNNGTC 1 cut(s) 1202
VspI ATTAAT 1 cut(s) 1428
XapI RAATTY 1 cut(s) 1071
XbaI TCTAGA 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 1163
XspI CTAG 5 cut(s) 120, 447, 474, 965, 1397
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.