FvH4_7g01430

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012410
Physical Location & Seq
Forward (+)
1842955 .. 1845146
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01430.t1

Sequence Viewer

Length: 1656 bp
ATGGTGGCCAAGAAACCAAGAATTGTTGTCATTGGGGCAGGAATGGCTGGTGTTACAGCAGCTAACAAGCTTTACACTTCTGGGTGTGAGGACTTGTTTGAGCTCATTGTTGTGGAAGGTGGGACTAGAATTGGAGGCAGAATAAACACTTCAGAGTTTGGTGGTGACCGGATTGAGATGGGTGCTACTTGGATCCATGGCATTGGAGGCAGCCCTCTTCACAAAATAGCTCAAGAAATCAAGGCCCTTGAGTCCGAGCAGCCATGGGAGTGCATGGACGGGTCATCAGACGAAGCCAAGACCATTGCTGAAGGTGGGTTCGAGGTGAGTCCGAATGTGGTGGATCCAATTACTAGTTTGTATAAGAATCTGATGGATTATGCTCAGGGGAAGAAGGTTGAGTATATGAAGCTTGGAGATGAAGCTGCTTCTGACGATGGTGGTGCAGGGAAGCTTAGTGTTGGTTCGTTTCTTCGAAAAGGGCTGGATGCTTATTGGGTTTCGAACAAGAGCAAGGAGGAGGTGAATGGTTGTGGTGAATGGAGCAGAAAGACGCTAGAAGATGCGGTTTTTGCAATGTATGAGAGCACCCAGAGGACTTATACTTCAGCTGGTGACTTGTTGACTCTGGATTATAATGCAGAAAGCGAGTACAGGATGTTCCCAGGTGAAGAAATCACCATTGCTAAAGGTTACTTGAGCATTGTTCAGTCCCTAGCCTCTGTTTTACCACCTGATGTGATCCAACTAGGCAAAAGGGTCACCAAGGTTGAATGGAAGGCAGAGGCTACTCATTTAGGTGTGGATCAAAATGGCTATGATTCAAGGCCTGTGAAGCTACACTTTAGTGATGGGTCAGTTTTGCTGGCTGATCATGTTATTGTTACTGTTTCTTTGGGGGTACTTAAAGCTGCAACACAGCAAGATTCAGGTATGTTCAGTCCTCCCTTGCCTTGTTTCAAGGCTGATGCAATATCAAGGCTTGGTTTTGGTGTTGTTAACAAGCTGTTTCTGCAACTGAGCTCCACCCATGTTACAAAAGGCCAGGATTTCAGCAAGTTTCCATTTTTGCAAATGGTGTTTCACAGAGAAGACTCAGAGTTCAGGCTTAAGAAGATTCCATGGTGGATGAGGAAGACAGCTTCAGTTTGTCCACTGTATCACAATTCCAGTGTACTATTGTCTTGGTTTGCAGGGAAAGAAGCACTTGAGCTTGAATCACTCAAAGATGAAGAGATCGTCAATGGAGTCACAGCAACAATCTCTAGCTTTCTATCAAAACCCCACAAGAAAGAGGAGAATTCTCATTCCCATTTGGGCAACGGGCATGTGAACCATGATGAGGAAAACTCTAGAGTGAAATTTTCCAAGGTGTTGAAAAGTCAATGGGGTAGTGATCCTTTGTTTCTTGGATCCTACTCTTATGTTGCTGTTGGATCAAGTGGTGAGGATTTGGACATCCTGGCAGAGCCATTGCCAAAGTTAAGCAATTGTGATGAGGCTGCTAGTGCTACTTCAACTTCTTCACTTCAAATTCTGTTTGCAGGGGAAGCAACACACAGAACTCACTATTCCACAACCCATGGAGCTTACTTTAGTGGTCTTAGAGAAGCCAATAGGCTTCTCCAATATTACAACCACCATGTTGGGGTTTAG

Protein Analysis

552

Amino Acids

60.32

Weight (kDa)

5.74

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 10 - 72 3.3e-09 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 15 - 542 6.7e-65 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AciI CCGC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 3 cut(s) 1300, 1361, 1533
AcuI CTGAAG 4 cut(s) 135, 330, 591, 1128
AfaI GTAC 3 cut(s) 653, 903, 1176
AfiI CCNNNNNNNGG 2 cut(s) 1342, 1648
AflII CTTAAG 1 cut(s) 1109
AgsI TTSAA 7 cut(s) 773, 825, 961, 1217, 1378, 1518, 1532
AhlI ACTAGT 1 cut(s) 353
AjnI CCWGG 3 cut(s) 664, 1044, 1461
AleI CACNNNNGTG 1 cut(s) 846
Alw21I GWGCWC 3 cut(s) 105, 590, 1025
AoxI GGCC 4 cut(s) 6, 243, 827, 1042
ApeKI GCWGC 6 cut(s) 59, 210, 259, 425, 911, 1502
ApoI RAATTY 3 cut(s) 1300, 1361, 1533
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 9 cut(s) 176, 337, 535, 548, 626, 670, 680, 754, 1457
AsuII TTCGAA 2 cut(s) 475, 503
BalI TGGCCA 1 cut(s) 8
BamHI GGATCC 3 cut(s) 192, 343, 1412
BanII GRGCYC 2 cut(s) 105, 1025
BbsI GAAGAC 2 cut(s) 1098, 1142
Bbv12I GWGCWC 3 cut(s) 105, 590, 1025
BbvI GCAGC 6 cut(s) 71, 222, 271, 412, 898, 1489
BccI CCATC 4 cut(s) 172, 367, 431, 845
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 3 cut(s) 666, 1046, 1463
BclI TGATCA 1 cut(s) 871
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 8 cut(s) 126, 354, 557, 716, 749, 1266, 1353, 1506
BfrI CTTAAG 1 cut(s) 1109
BisI GCNGC 6 cut(s) 60, 211, 260, 426, 912, 1503
BlsI GCNGC 6 cut(s) 61, 212, 261, 427, 913, 1504
Bme1390I CCNGG 3 cut(s) 666, 1046, 1463
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 3 cut(s) 194, 345, 1414
BmrFI CCNGG 3 cut(s) 666, 1046, 1463
BmsI GCATC 3 cut(s) 478, 553, 958
BpiI GAAGAC 2 cut(s) 1098, 1142
BplI GAGNNNNNCTC 2 cut(s) 1334, 1366
Bpu10I CCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 2 cut(s) 475, 503
BpuEI CTTGAG 4 cut(s) 216, 269, 718, 1229
BsaJI CCNNGG 7 cut(s) 196, 263, 664, 765, 1121, 1368, 1582
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 2 cut(s) 1342, 1648
Bse1I ACTGG 1 cut(s) 1170
Bse3DI GCAATG 4 cut(s) 303, 582, 681, 1472
BseBI CCWGG 3 cut(s) 666, 1046, 1463
BseDI CCNNGG 7 cut(s) 196, 263, 664, 765, 1121, 1368, 1582
BseGI GGATG 4 cut(s) 493, 663, 1134, 1458
BseLI CCNNNNNNNGG 2 cut(s) 1342, 1648
BseMI GCAATG 4 cut(s) 303, 582, 681, 1472
BseMII CTCAG 3 cut(s) 398, 1010, 1110
BseNI ACTGG 1 cut(s) 1170
BseRI GAGGAG 2 cut(s) 533, 1310
BseXI GCAGC 6 cut(s) 71, 222, 271, 412, 898, 1489
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 4 cut(s) 8, 245, 829, 1044
BsiHKAI GWGCWC 3 cut(s) 105, 590, 1025
BsiSI CCGG 1 cut(s) 169
BslFI GGGAC 2 cut(s) 136, 697
BslI CCNNNNNNNGG 2 cut(s) 1342, 1648
BsmFI GGGAC 2 cut(s) 136, 697
BsnI GGCC 4 cut(s) 8, 245, 829, 1044
Bsp119I TTCGAA 2 cut(s) 475, 503
Bsp1286I GDGCHC 3 cut(s) 105, 590, 1025
Bsp143I GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
Bsp19I CCATGG 4 cut(s) 196, 263, 1121, 1582
BspACI CCGC 1 cut(s) 566
BspANI GGCC 4 cut(s) 8, 245, 829, 1044
BspCNI CTCAG 3 cut(s) 397, 1011, 1109
BspLI GGNNCC 3 cut(s) 194, 345, 1414
BspT104I TTCGAA 2 cut(s) 475, 503
BspTI CTTAAG 1 cut(s) 1109
BsrDI GCAATG 4 cut(s) 303, 582, 681, 1472
BsrI ACTGG 1 cut(s) 1170
BssECI CCNNGG 7 cut(s) 196, 263, 664, 765, 1121, 1368, 1582
BssMI GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
BssT1I CCWWGG 6 cut(s) 196, 263, 765, 1121, 1368, 1582
Bst2UI CCWGG 3 cut(s) 666, 1046, 1463
Bst4CI ACNGT 2 cut(s) 889, 1158
Bst6I CTCTTC 2 cut(s) 222, 1227
BstAFI CTTAAG 1 cut(s) 1109
BstBI TTCGAA 2 cut(s) 475, 503
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 5 cut(s) 384, 455, 1019, 1096, 1604
BstDSI CCRYGG 4 cut(s) 196, 263, 1121, 1582
BstEII GGTNACC 2 cut(s) 164, 760
BstF5I GGATG 4 cut(s) 493, 663, 1134, 1458
BstKTI GATC 9 cut(s) 195, 346, 744, 808, 874, 1239, 1399, 1415, 1439
BstMBI GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
BstMWI GCNNNNNNNGC 7 cut(s) 44, 207, 572, 835, 1012, 1508, 1550
BstNI CCWGG 3 cut(s) 666, 1046, 1463
BstNSI RCATGY 1 cut(s) 1331
BstPI GGTNACC 2 cut(s) 164, 760
BstSCI CCNGG 3 cut(s) 664, 1044, 1461
BstV1I GCAGC 6 cut(s) 71, 222, 271, 412, 898, 1489
BstV2I GAAGAC 2 cut(s) 1098, 1142
BstX2I RGATCY 3 cut(s) 192, 343, 1412
BstXI CCANNNNNNTGG 2 cut(s) 203, 1646
BstYI RGATCY 3 cut(s) 192, 343, 1412
BsuRI GGCC 4 cut(s) 8, 245, 829, 1044
BtgI CCRYGG 4 cut(s) 196, 263, 1121, 1582
BtsCI GGATG 4 cut(s) 493, 663, 1134, 1458
BtsIMutI CAGTG 2 cut(s) 1154, 1177
Cac8I GCNNGC 1 cut(s) 867
Cfr13I GGNCC 1 cut(s) 244
CseI GACGC 1 cut(s) 562
Csp6I GTAC 3 cut(s) 652, 902, 1175
CviQI GTAC 3 cut(s) 652, 902, 1175
DdeI CTNAG 5 cut(s) 384, 455, 1019, 1096, 1604
DpnI GATC 9 cut(s) 194, 345, 743, 807, 873, 1238, 1398, 1414, 1438
DpnII GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 222, 1227
EarI CTCTTC 2 cut(s) 222, 1227
Ecl136II GAGCTC 2 cut(s) 103, 1023
Eco130I CCWWGG 6 cut(s) 196, 263, 765, 1121, 1368, 1582
Eco147I AGGCCT 1 cut(s) 829
Eco24I GRGCYC 2 cut(s) 105, 1025
Eco53kI GAGCTC 2 cut(s) 103, 1023
Eco57I CTGAAG 4 cut(s) 135, 330, 591, 1128
Eco91I GGTNACC 2 cut(s) 164, 760
EcoICRI GAGCTC 2 cut(s) 103, 1023
EcoO109I RGGNCCY 1 cut(s) 244
EcoO65I GGTNACC 2 cut(s) 164, 760
EcoRI GAATTC 1 cut(s) 1300
EcoRII CCWGG 3 cut(s) 664, 1044, 1461
EcoT14I CCWWGG 6 cut(s) 196, 263, 765, 1121, 1368, 1582
EcoT38I GRGCYC 2 cut(s) 105, 1025
ErhI CCWWGG 6 cut(s) 196, 263, 765, 1121, 1368, 1582
FalI AAGNNNNNCTT 4 cut(s) 827, 859, 1191, 1223
FaqI GGGAC 2 cut(s) 136, 697
FbaI TGATCA 1 cut(s) 871
Fnu4HI GCNGC 6 cut(s) 60, 211, 260, 426, 912, 1503
FokI GGATG 4 cut(s) 500, 670, 1141, 1445
FriOI GRGCYC 2 cut(s) 105, 1025
Fsp4HI GCNGC 6 cut(s) 60, 211, 260, 426, 912, 1503
FspBI CTAG 8 cut(s) 126, 354, 557, 716, 749, 1266, 1353, 1506
GluI GCNGC 6 cut(s) 60, 211, 260, 426, 912, 1503
HaeIII GGCC 4 cut(s) 8, 245, 829, 1044
HapII CCGG 1 cut(s) 169
HgaI GACGC 1 cut(s) 562
HincII GTYRAC 2 cut(s) 624, 1000
HindII GTYRAC 2 cut(s) 624, 1000
HindIII AAGCTT 3 cut(s) 68, 410, 452
HpaI GTTAAC 1 cut(s) 1000
HpaII CCGG 1 cut(s) 169
HphI GGTGA 9 cut(s) 176, 337, 535, 548, 626, 670, 680, 754, 1457
Hpy166II GTNNAC 5 cut(s) 624, 1000, 1154, 1175, 1333
Hpy188I TCNGA 7 cut(s) 154, 256, 289, 333, 372, 433, 1099
Hpy188III TCNNGA 3 cut(s) 233, 629, 1353
Hpy8I GTNNAC 5 cut(s) 624, 1000, 1154, 1175, 1333
HpyAV CCTTC 4 cut(s) 110, 305, 388, 772
HpyCH4III ACNGT 2 cut(s) 889, 1158
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 207, 572, 835, 1012, 1508, 1550
HpyF3I CTNAG 5 cut(s) 384, 455, 1019, 1096, 1604
Ksp22I TGATCA 1 cut(s) 871
KspAI GTTAAC 1 cut(s) 1000
Kzo9I GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
LmnI GCTCC 3 cut(s) 543, 1028, 1586
Lsp1109I GCAGC 6 cut(s) 71, 222, 271, 412, 898, 1489
LweI GCATC 3 cut(s) 478, 553, 958
MaeI CTAG 8 cut(s) 126, 354, 557, 716, 749, 1266, 1353, 1506
MaeIII GTNAC 8 cut(s) 52, 164, 614, 692, 760, 883, 1033, 1249
MalI GATC 9 cut(s) 194, 345, 743, 807, 873, 1238, 1398, 1414, 1438
MboI GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
MfeI CAATTG 1 cut(s) 1489
MflI RGATCY 3 cut(s) 192, 343, 1412
MhlI GDGCHC 3 cut(s) 105, 590, 1025
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 8 cut(s) 21, 129, 348, 1165, 1300, 1361, 1489, 1533
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 5 cut(s) 260, 337, 619, 1088, 1257
MmeI TCCRAC 2 cut(s) 769, 1414
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 4 cut(s) 906, 999, 1110, 1484
MslI CAYNNNNRTG 4 cut(s) 110, 268, 798, 846
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 1109
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 3 cut(s) 666, 1046, 1463
MunI CAATTG 1 cut(s) 1489
MvaI CCWGG 3 cut(s) 666, 1046, 1463
MwoI GCNNNNNNNGC 7 cut(s) 44, 207, 572, 835, 1012, 1508, 1550
NcoI CCATGG 4 cut(s) 196, 263, 1121, 1582
NdeII GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
NlaIV GGNNCC 3 cut(s) 194, 345, 1414
NmuCI GTSAC 4 cut(s) 164, 614, 760, 1249
NspI RCATGY 1 cut(s) 1331
NspV TTCGAA 2 cut(s) 475, 503
OliI CACNNNNGTG 1 cut(s) 846
PceI AGGCCT 1 cut(s) 829
PfeI GAWTC 5 cut(s) 367, 821, 926, 1117, 1217
PkrI GCNGC 6 cut(s) 61, 212, 261, 427, 913, 1504
PleI GAGTC 5 cut(s) 259, 336, 619, 1088, 1256
PpsI GAGTC 5 cut(s) 259, 336, 619, 1088, 1256
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 2 cut(s) 105, 1025
Psp6I CCWGG 3 cut(s) 664, 1044, 1461
PspEI GGTNACC 2 cut(s) 164, 760
PspGI CCWGG 3 cut(s) 664, 1044, 1461
PspN4I GGNNCC 3 cut(s) 194, 345, 1414
PspPI GGNCC 1 cut(s) 244
PsrI GAACNNNNNNTAC 2 cut(s) 644, 676
PsuI RGATCY 3 cut(s) 192, 343, 1412
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 653, 903, 1176
RsaNI GTAC 3 cut(s) 652, 902, 1175
RseI CAYNNNNRTG 4 cut(s) 110, 268, 798, 846
SacI GAGCTC 2 cut(s) 105, 1025
SaqAI TTAA 4 cut(s) 906, 999, 1110, 1484
SatI GCNGC 6 cut(s) 60, 211, 260, 426, 912, 1503
Sau3AI GATC 9 cut(s) 192, 343, 741, 805, 871, 1236, 1396, 1412, 1436
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 5 cut(s) 260, 337, 619, 1088, 1257
ScrFI CCNGG 3 cut(s) 666, 1046, 1463
SduI GDGCHC 3 cut(s) 105, 590, 1025
SfaNI GCATC 3 cut(s) 478, 553, 958
SfuI TTCGAA 2 cut(s) 475, 503
SmiMI CAYNNNNRTG 4 cut(s) 110, 268, 798, 846
SmlI CTYRAG 5 cut(s) 231, 248, 697, 1109, 1208
SmoI CTYRAG 5 cut(s) 231, 248, 697, 1109, 1208
SpeI ACTAGT 1 cut(s) 353
Sse9I AATT 8 cut(s) 21, 129, 348, 1165, 1300, 1361, 1489, 1533
SseBI AGGCCT 1 cut(s) 829
SsiI CCGC 1 cut(s) 566
SspI AATATT 1 cut(s) 1631
SspMI CTAG 8 cut(s) 126, 354, 557, 716, 749, 1266, 1353, 1506
SstI GAGCTC 2 cut(s) 105, 1025
StuI AGGCCT 1 cut(s) 829
StyD4I CCNGG 3 cut(s) 664, 1044, 1461
StyI CCWWGG 6 cut(s) 196, 263, 765, 1121, 1368, 1582
TaaI ACNGT 2 cut(s) 889, 1158
TaqI TCGA 3 cut(s) 321, 475, 503
TasI AATT 8 cut(s) 21, 129, 348, 1165, 1300, 1361, 1489, 1533
TatI WGTACW 2 cut(s) 651, 1174
TfiI GAWTC 5 cut(s) 367, 821, 926, 1117, 1217
Tru1I TTAA 4 cut(s) 906, 999, 1110, 1484
Tru9I TTAA 4 cut(s) 906, 999, 1110, 1484
TscAI CASTG 2 cut(s) 1161, 1177
TseFI GTSAC 4 cut(s) 164, 614, 760, 1249
TseI GCWGC 6 cut(s) 59, 210, 259, 425, 911, 1502
Tsp45I GTSAC 4 cut(s) 164, 614, 760, 1249
TspDTI ATGAA 3 cut(s) 422, 435, 1245
TspRI CASTG 2 cut(s) 1161, 1177
Vha464I CTTAAG 1 cut(s) 1109
XapI RAATTY 3 cut(s) 1300, 1361, 1533
XbaI TCTAGA 1 cut(s) 1352
XceI RCATGY 1 cut(s) 1331
XspI CTAG 8 cut(s) 126, 354, 557, 716, 749, 1266, 1353, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.