MD01G1012400.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012410
Physical Location & Seq
Forward (+)
6370131 .. 6370953
823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1012400.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGACGGGTCTTCCGACGAGCCCACCACCGTCGCGAAATGTGGGTTGGAGCTGGAACCGGATGTGGTGGACCCCATTTCGTCTCTGTTCAAGAACTTGATGGATTATGCTCAGGGGAAGCAGGTGTTTGATGAAAGTCCAGAGAGAGAGTGCAATGGTGATTTTGAATACAGTAAGCTCGGAGATGACGCTTCCAGGATTTGTGCAAGCAATGACGGTGTTGGGAAGCTCAGTGTCAGTGGCTATACAAATTGGAGCAGAAAGTTGCTGCAAGATACCATCTTTGCAATGCATGAGAACATCCAAAGGACTTATACATCAGCCGGTGATTTGTTAACTCTCGATTACAATGCAGAAAACGAGTACCGGATGTTTCCCGGCGAAGAAATCATCATTGCTAAAGGGTACTTGAGCATTGTTCAATCTCTAGCCTCTGTACTCCCACCTGGGTTGATCCAATTGGGCAAGAAGGTCAGAAAAATTCAATGGCAGCCGGATAATCGCAAGAACAAGGGCTATGAATCCGACACAAGGCTTGTGAAGCTCCATTTTTCTGATGGGTCAGTTTTGTTAGCTGATCATGTTATTGTTACAGTTTCTTTGGGGGTGCTCAAAGCTTCGATTTTCCAAGATTCTGCTGTTTCTGCAACTGGGTTCTATTCATGCTCCTCCGGCATTTGA

Protein Analysis

227

Amino Acids

24.82

Weight (kDa)

4.85

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amino_oxidase PF01593 92 - 211 1.1e-14 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 112
Acc36I ACCTGC 1 cut(s) 112
AccII CGCG 1 cut(s) 35
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 480
AfaI GTAC 3 cut(s) 365, 407, 438
AfiI CCNNNNNNNGG 1 cut(s) 531
AgsI TTSAA 4 cut(s) 91, 167, 422, 485
AjnI CCWGG 2 cut(s) 194, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 6 cut(s) 52, 178, 229, 544, 575, 617
AluI AGCT 6 cut(s) 52, 178, 229, 544, 575, 617
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 268, 490
ApoI RAATTY 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 170, 338
AvaII GGWCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 255, 502
BccI CCATC 3 cut(s) 94, 287, 551
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 196, 447
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 87
BfaI CTAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 112
BisI GCNGC 2 cut(s) 269, 491
BlsI GCNGC 2 cut(s) 270, 492
Bme1390I CCNGG 3 cut(s) 196, 378, 447
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 57, 72
BmrFI CCNGG 3 cut(s) 196, 378, 447
BmrI ACTGGG 1 cut(s) 660
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 3
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 430
BpuMI CCSGG 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 446
BsaWI WCCGGW 2 cut(s) 58, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 4 cut(s) 160, 217, 294, 393
BseBI CCWGG 2 cut(s) 196, 447
BseDI CCNNGG 1 cut(s) 446
BseGI GGATG 3 cut(s) 67, 300, 375
BseLI CCNNNNNNNGG 1 cut(s) 531
BseMI GCAATG 4 cut(s) 160, 217, 294, 393
BseMII CTCAG 2 cut(s) 125, 244
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 658
BseXI GCAGC 2 cut(s) 255, 502
Bsh1236I CGCG 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 6 cut(s) 59, 324, 367, 378, 494, 672
BslI CCNNNNNNNGG 1 cut(s) 531
BsmAI GTCTC 1 cut(s) 87
BsmBI CGTCTC 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 24, 612
Bsp143I GATC 2 cut(s) 453, 577
Bsp68I TCGCGA 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 124, 243
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 2 cut(s) 57, 72
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 4 cut(s) 160, 217, 294, 393
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 446
BssMI GATC 2 cut(s) 453, 577
Bst2UI CCWGG 2 cut(s) 196, 447
Bst4CI ACNGT 4 cut(s) 31, 173, 218, 595
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 111, 230
BstF5I GGATG 3 cut(s) 67, 300, 375
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 2 cut(s) 456, 580
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 2 cut(s) 453, 577
BstMWI GCNNNNNNNGC 3 cut(s) 541, 644, 672
BstNI CCWGG 2 cut(s) 196, 447
BstSCI CCNGG 3 cut(s) 194, 376, 445
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 255, 502
BstV2I GAAGAC 1 cut(s) 3
BtsCI GGATG 3 cut(s) 67, 300, 375
BtsIMutI CAGTG 2 cut(s) 238, 244
BtuMI TCGCGA 1 cut(s) 35
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 70
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 364, 406, 437
CspCI CAANNNNNGTGG 2 cut(s) 432, 467
CviAII CATG 3 cut(s) 293, 581, 663
CviQI GTAC 3 cut(s) 364, 406, 437
DdeI CTNAG 2 cut(s) 111, 230
DpnI GATC 2 cut(s) 455, 579
DpnII GATC 2 cut(s) 453, 577
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 194, 445
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 3 cut(s) 296, 584, 666
FaiI YATR 7 cut(s) 108, 246, 294, 315, 519, 582, 664
FatI CATG 3 cut(s) 292, 580, 662
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 269, 491
FokI GGATG 3 cut(s) 74, 287, 382
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 269, 491
FspBI CTAG 1 cut(s) 428
GluI GCNGC 2 cut(s) 269, 491
HapII CCGG 6 cut(s) 59, 324, 367, 378, 494, 672
HgaI GACGC 1 cut(s) 197
Hin1II CATG 3 cut(s) 296, 584, 666
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 2 cut(s) 521, 632
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 6 cut(s) 59, 324, 367, 378, 494, 672
HphI GGTGA 2 cut(s) 170, 338
Hpy166II GTNNAC 2 cut(s) 70, 336
Hpy188I TCNGA 5 cut(s) 16, 182, 476, 526, 556
Hpy188III TCNNGA 4 cut(s) 34, 91, 140, 341
Hpy8I GTNNAC 2 cut(s) 70, 336
Hpy99I CGWCG 2 cut(s) 20, 35
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 4 cut(s) 31, 173, 218, 595
HpyCH4V TGCA 7 cut(s) 153, 206, 271, 287, 292, 353, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 541, 644, 672
HpyF3I CTNAG 2 cut(s) 111, 230
Hsp92II CATG 3 cut(s) 296, 584, 666
Ksp22I TGATCA 1 cut(s) 577
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 2 cut(s) 453, 577
LmnI GCTCC 4 cut(s) 49, 255, 549, 671
Lsp1109I GCAGC 2 cut(s) 255, 502
MaeI CTAG 1 cut(s) 428
MaeIII GTNAC 1 cut(s) 589
MalI GATC 2 cut(s) 455, 579
MboI GATC 2 cut(s) 453, 577
MboII GAAGA 2 cut(s) 3, 395
MfeI CAATTG 1 cut(s) 458
MhlI GDGCHC 2 cut(s) 24, 612
MluCI AATT 3 cut(s) 250, 458, 480
MmeI TCCRAC 3 cut(s) 27, 39, 549
MnlI CCTC 2 cut(s) 442, 679
Mph1103I ATGCAT 1 cut(s) 294
MseI TTAA 1 cut(s) 335
MspI CCGG 6 cut(s) 59, 324, 367, 378, 494, 672
MspR9I CCNGG 3 cut(s) 196, 378, 447
MunI CAATTG 1 cut(s) 458
MvaI CCWGG 2 cut(s) 196, 447
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 541, 644, 672
NciI CCSGG 1 cut(s) 378
NdeII GATC 2 cut(s) 453, 577
NlaIII CATG 3 cut(s) 296, 584, 666
NlaIV GGNNCC 2 cut(s) 57, 72
NruI TCGCGA 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 294
PaqCI CACCTGC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 2 cut(s) 521, 632
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 2 cut(s) 270, 492
Psp6I CCWGG 2 cut(s) 194, 445
PspGI CCWGG 2 cut(s) 194, 445
PspN4I GGNNCC 2 cut(s) 57, 72
PspPI GGNCC 1 cut(s) 70
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 3 cut(s) 365, 407, 438
RsaNI GTAC 3 cut(s) 364, 406, 437
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 269, 491
Sau3AI GATC 2 cut(s) 453, 577
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 3 cut(s) 196, 378, 447
SduI GDGCHC 2 cut(s) 24, 612
SetI ASST 9 cut(s) 54, 126, 180, 231, 448, 474, 546, 577, 619
SinI GGWCC 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 3 cut(s) 250, 458, 480
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 194, 376, 445
TaaI ACNGT 4 cut(s) 31, 173, 218, 595
TaqI TCGA 2 cut(s) 342, 620
TasI AATT 3 cut(s) 250, 458, 480
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 2 cut(s) 521, 632
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 238, 244
TseI GCWGC 2 cut(s) 268, 490
TspDTI ATGAA 3 cut(s) 147, 534, 651
TspRI CASTG 2 cut(s) 238, 244
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 1 cut(s) 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.