MD02G1079400.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012410
Physical Location & Seq
Reverse (-)
6259023 .. 6260699
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1079400.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGTGGCGAAGAAATCACGGGTTGTGATCATTGGAGCGGGAATGGCCGGCGTCACGGCGGCGAACAAGCTGTACACTGCTGCTGGGTCCAAGGACTTGTTCGAGCTCGTGGTTGTGGAAGGCGGGGAGAGAATTGGCGGCAGAATCAACACGTCGGAGTTCGGCGGTGACCGGATTGAGATGGGAGCTACCTGGATCCACGGCATTGAAGGTAGCCCGGTTCATAAAATTGCTCAGGAAATCAATGCGCTCGAGTCGGAGCAGCCGTGGGAGTGCATGGACGGGTCCTCCGACGAGCCCACCACCGTCGCCGAAGGTGGGTTGGTGCTGGAACCGGATGTGGTGGACCGCATTTCGTCTCTCTTCAAGAACTTGATGGATTATGCTCAGGGGAAGAAGGTGTTTGATGAAAGTCCTGAAATAGTGTGCAATGGCGAATTTGAATATGGTAAGCTTGGAGAAGAAGCTTCCAGGATTTGTGCAAGCAATGGCGGTGTTGGGAAGCTCAGTGTCGGCTCTTTCTTAAGGCGAGGTCTTGATGCGTATTGGGTTTTGACGAAAAACCGAGATGAGCAGGTCAATGGCAATGGAAATTGGAGCAGAAAGTTGCTGCAAGAAGCCATCTTTGCAATGCATGAGAACACCCAAAGGACGTATACATCAGCTGGTGACTTGTTAACTCTTGATTACAATGCTGAAAGTGAGTACCGGATGTTTCCCGGCGAAGAAATTACCATTGCTAAAGGGTACTTGAGCATTGTTCAATCTCTAGCCTCTGTTCTCCCACCTGGGTTGATTCAATTAGGCAAAAAGGTCACAGAAATTCAATGGCAGCCGGAAAATCACACGCACAGCGGCTATGAATCTGACACAAGGCCCGTGAAGCTCCATTTTTCTGATGGGTCAGTTTTATTAGCCAATCATGTCATTATCACAGTTTCTTTGGGGGTGCTCAAAGCTTCGATTCGCCAAGATTCTGGTATGTTCAATCCACCTCTGCCTCGTTTCAAAACCGAGGCGATTTCGAGGCTTGGATTTGGCGTTGTTAACAAGCTGTTTCTGCAACTGGGTTCCAATCATCCTCCTCTGAAGTCCCATGATTTCAGCAAGTTTCCATTTTTACAAATGGTTTTCCACAGAGCAGACTCGGAGCTCAGGAATAAAAAGATCCCATGGTGGATGAGGAAGACGGCTTCTCTCTGCCCTCTCTACCACAATTCCAACGTTCTGCTTTCTTGGTTTGCAGGGAAAGAAGCACTTGCGCTAGAAGCACTTTCAGGTGAAGAGATTATCAATGGGGTTTCACAAACGGTCTCCAGCTTCCTATCACACACATCACACTCCCATGAATCGTGCAATGGGAACGGGAATCCAGAGGGGAATTCAGTACAATTTTCCAAGGTTTTGAAGAGTCAATGGGCTAGTGATCCTCTGTTTCTGGGATCATACTCATATGTAGCAGTTGGATCAAGCGGTGAGGATTTGGACAGCATGGCAGAGCCATTGCCATCTGATGATTCTGGCAGTGCCACTAGTTCTTCACCTCCTCCACTTCAAATTTTATTTGCAGGGGAGGCTACACACAGAACCCATTACTCTACAACCCATGGAGCTTACTTGAGTGGCCTCAGGGAAGCCAATAGGCTTCTCCAACATTACCAACATGTTGGGGTTTAG

Protein Analysis

559

Amino Acids

60.9

Weight (kDa)

5.77

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 10 - 76 6.9e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 15 - 550 3.4e-63 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 656
AciI CCGC 9 cut(s) 38, 59, 123, 138, 165, 349, 492, 855, 1473
AclI AACGTT 1 cut(s) 1224
AclWI GGATC 6 cut(s) 190, 203, 1162, 1421, 1450, 1474
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 4 cut(s) 437, 822, 1381, 1557
AcuI CTGAAG 1 cut(s) 1109
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 4 cut(s) 74, 707, 749, 1389
AfiI CCNNNNNNNGG 1 cut(s) 318
AflII CTTAAG 1 cut(s) 523
AflIII ACRYGT 2 cut(s) 150, 1663
AhlI ACTAGT 1 cut(s) 1532
AjiI CACGTC 1 cut(s) 153
AjnI CCWGG 3 cut(s) 191, 470, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
Alw21I GWGCWC 3 cut(s) 108, 954, 1155
Alw26I GTCTC 2 cut(s) 363, 1318
AlwI GGATC 6 cut(s) 190, 203, 1162, 1421, 1450, 1474
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 3 cut(s) 45, 875, 1624
ApeKI GCWGC 4 cut(s) 80, 262, 610, 832
ApoI RAATTY 4 cut(s) 437, 822, 1381, 1557
AspLEI GCGC 2 cut(s) 250, 1264
AspS9I GGNCC 4 cut(s) 87, 285, 346, 876
AsuC2I CCSGG 2 cut(s) 218, 720
AsuHPI GGTGA 5 cut(s) 179, 680, 1292, 1487, 1533
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 87, 285, 346
AxyI CCTNAGG 1 cut(s) 1628
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 3 cut(s) 108, 300, 1155
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 1193
Bbv12I GWGCWC 3 cut(s) 108, 954, 1155
BbvI GCAGC 4 cut(s) 67, 274, 597, 844
BccI CCATC 5 cut(s) 175, 370, 629, 893, 1516
BceAI ACGGC 4 cut(s) 72, 217, 250, 1206
BciT130I CCWGG 3 cut(s) 193, 472, 789
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 2 cut(s) 218, 720
BcoDI GTCTC 2 cut(s) 363, 1318
BcuI ACTAGT 1 cut(s) 1532
BfaI CTAG 4 cut(s) 770, 1265, 1422, 1533
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 7 cut(s) 60, 81, 139, 263, 611, 833, 856
BlsI GCNGC 7 cut(s) 61, 82, 140, 264, 612, 834, 857
Bme1390I CCNGG 5 cut(s) 193, 218, 472, 720, 789
Bme18I GGWCC 3 cut(s) 87, 285, 346
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 4 cut(s) 87, 285, 346, 876
BmiI GGNNCC 5 cut(s) 88, 197, 286, 333, 1072
BmrFI CCNGG 5 cut(s) 193, 218, 472, 720, 789
BmrI ACTGGG 1 cut(s) 1076
BmsI GCATC 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 1076
BpiI GAAGAC 1 cut(s) 1193
BpmI CTGGAG 1 cut(s) 1300
Bpu10I CCTNAGC 3 cut(s) 234, 387, 1154
BpuEI CTTGAG 2 cut(s) 772, 1639
BpuMI CCSGG 2 cut(s) 218, 720
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 1318
BsaJI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1398, 1606
BsaWI WCCGGW 3 cut(s) 171, 334, 708
BsaXI ACNNNNNCTCC 4 cut(s) 251, 272, 281, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 1071
Bse21I CCTNAGG 1 cut(s) 1628
Bse3DI GCAATG 7 cut(s) 436, 493, 592, 636, 735, 1363, 1502
BseBI CCWGG 3 cut(s) 193, 472, 789
BseDI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1398, 1606
BseGI GGATG 4 cut(s) 343, 717, 1078, 1185
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 7 cut(s) 436, 493, 592, 636, 735, 1363, 1502
BseMII CTCAG 5 cut(s) 248, 401, 520, 1168, 1642
BseNI ACTGG 1 cut(s) 1071
BseRI GAGGAG 2 cut(s) 1074, 1536
BseXI GCAGC 4 cut(s) 67, 274, 597, 844
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 3 cut(s) 47, 877, 1626
BsiHKAI GWGCWC 3 cut(s) 108, 954, 1155
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 7 cut(s) 48, 172, 218, 335, 709, 720, 836
BslFI GGGAC 1 cut(s) 1078
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 2 cut(s) 363, 1318
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 1078
BsnI GGCC 3 cut(s) 47, 877, 1626
Bso31I GGTCTC 1 cut(s) 1318
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 4 cut(s) 108, 300, 954, 1155
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
Bsp19I CCATGG 2 cut(s) 1172, 1606
BspACI CCGC 9 cut(s) 38, 59, 123, 138, 165, 349, 492, 855, 1473
BspANI GGCC 3 cut(s) 47, 877, 1626
BspCNI CTCAG 5 cut(s) 247, 400, 519, 1167, 1641
BspLI GGNNCC 5 cut(s) 88, 197, 286, 333, 1072
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 6 cut(s) 190, 203, 1162, 1421, 1450, 1474
BspTI CTTAAG 1 cut(s) 523
BspTNI GGTCTC 1 cut(s) 1318
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 7 cut(s) 436, 493, 592, 636, 735, 1363, 1502
BsrFI RCCGGY 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 1071
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1398, 1606
BssMI GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
BssNAI GTATAC 1 cut(s) 657
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 4 cut(s) 90, 1172, 1398, 1606
Bst1107I GTATAC 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 3 cut(s) 193, 472, 789
Bst4CI ACNGT 3 cut(s) 307, 937, 1312
Bst6I CTCTTC 3 cut(s) 368, 1278, 1403
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 523
BstAUI TGTACA 1 cut(s) 72
BstC8I GCNNGC 2 cut(s) 49, 484
BstDEI CTNAG 5 cut(s) 234, 387, 506, 1154, 1628
BstDSI CCRYGG 4 cut(s) 199, 266, 1172, 1606
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 4 cut(s) 343, 717, 1078, 1185
BstHHI GCGC 2 cut(s) 250, 1264
BstKTI GATC 6 cut(s) 30, 198, 1170, 1429, 1445, 1469
BstMAI GTCTC 2 cut(s) 363, 1318
BstMBI GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
BstMWI GCNNNNNNNGC 6 cut(s) 44, 626, 883, 1060, 1268, 1574
BstNI CCWGG 3 cut(s) 193, 472, 789
BstNSI RCATGY 1 cut(s) 1667
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 5 cut(s) 191, 216, 470, 718, 787
BstV1I GCAGC 4 cut(s) 67, 274, 597, 844
BstV2I GAAGAC 1 cut(s) 1193
BstX2I RGATCY 2 cut(s) 195, 1167
BstXI CCANNNNNNTGG 2 cut(s) 977, 1667
BstYI RGATCY 2 cut(s) 195, 1167
BstZ17I GTATAC 1 cut(s) 657
Bsu36I CCTNAGG 1 cut(s) 1628
BsuRI GGCC 3 cut(s) 47, 877, 1626
BtgI CCRYGG 4 cut(s) 199, 266, 1172, 1606
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 4 cut(s) 343, 717, 1078, 1185
BtsI GCAGTG 2 cut(s) 75, 1531
BtsIMutI CAGTG 3 cut(s) 75, 514, 1531
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 49, 484
CfoI GCGC 2 cut(s) 250, 1264
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 4 cut(s) 87, 285, 346, 876
CseI GACGC 1 cut(s) 40
Csp6I GTAC 4 cut(s) 73, 706, 748, 1388
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviAII CATG 9 cut(s) 277, 635, 923, 1097, 1173, 1346, 1492, 1607, 1664
CviQI GTAC 4 cut(s) 73, 706, 748, 1388
DdeI CTNAG 5 cut(s) 234, 387, 506, 1154, 1628
DpnI GATC 6 cut(s) 29, 197, 1169, 1428, 1444, 1468
DpnII GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 368, 1278, 1403
EarI CTCTTC 3 cut(s) 368, 1278, 1403
Ecl136II GAGCTC 2 cut(s) 106, 1153
Eco130I CCWWGG 4 cut(s) 90, 1172, 1398, 1606
Eco24I GRGCYC 3 cut(s) 108, 300, 1155
Eco31I GGTCTC 1 cut(s) 1318
Eco47I GGWCC 3 cut(s) 87, 285, 346
Eco53kI GAGCTC 2 cut(s) 106, 1153
Eco57I CTGAAG 1 cut(s) 1109
Eco81I CCTNAGG 1 cut(s) 1628
Eco88I CYCGRG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 2 cut(s) 106, 1153
EcoO109I RGGNCCY 1 cut(s) 285
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 1381
EcoRII CCWGG 3 cut(s) 191, 470, 787
EcoT14I CCWWGG 4 cut(s) 90, 1172, 1398, 1606
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 3 cut(s) 108, 300, 1155
ErhI CCWWGG 4 cut(s) 90, 1172, 1398, 1606
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 9 cut(s) 280, 638, 926, 1100, 1176, 1349, 1495, 1610, 1667
FalI AAGNNNNNCTT 2 cut(s) 1242, 1274
FaqI GGGAC 1 cut(s) 1078
FatI CATG 9 cut(s) 276, 634, 922, 1096, 1172, 1345, 1491, 1606, 1663
FauI CCCGC 2 cut(s) 31, 116
FauNDI CATATG 1 cut(s) 1453
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 7 cut(s) 60, 81, 139, 263, 611, 833, 856
FokI GGATG 4 cut(s) 350, 724, 1065, 1192
FriOI GRGCYC 3 cut(s) 108, 300, 1155
Fsp4HI GCNGC 7 cut(s) 60, 81, 139, 263, 611, 833, 856
FspBI CTAG 4 cut(s) 770, 1265, 1422, 1533
GlaI GCGC 2 cut(s) 249, 1263
GluI GCNGC 7 cut(s) 60, 81, 139, 263, 611, 833, 856
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 1300
HaeIII GGCC 3 cut(s) 47, 877, 1626
HapII CCGG 7 cut(s) 48, 172, 218, 335, 709, 720, 836
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 250, 1264
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 9 cut(s) 280, 638, 926, 1100, 1176, 1349, 1495, 1610, 1667
Hin6I GCGC 2 cut(s) 248, 1262
HinP1I GCGC 2 cut(s) 248, 1262
HincII GTYRAC 2 cut(s) 678, 1048
HindII GTYRAC 2 cut(s) 678, 1048
HindIII AAGCTT 3 cut(s) 452, 465, 957
HpaI GTTAAC 2 cut(s) 678, 1048
HpaII CCGG 7 cut(s) 48, 172, 218, 335, 709, 720, 836
HphI GGTGA 5 cut(s) 179, 680, 1292, 1487, 1533
Hpy166II GTNNAC 5 cut(s) 75, 346, 657, 678, 1048
Hpy188I TCNGA 8 cut(s) 157, 259, 292, 868, 898, 1089, 1150, 1513
Hpy188III TCNNGA 7 cut(s) 236, 367, 416, 536, 683, 1156, 1373
Hpy8I GTNNAC 5 cut(s) 75, 346, 657, 678, 1048
Hpy99I CGWCG 3 cut(s) 157, 296, 311
HpyAV CCTTC 4 cut(s) 113, 203, 308, 391
HpyCH4III ACNGT 3 cut(s) 307, 937, 1312
HpyCH4IV ACGT 3 cut(s) 152, 653, 1224
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 626, 883, 1060, 1268, 1574
HpyF3I CTNAG 5 cut(s) 234, 387, 506, 1154, 1628
HpySE526I ACGT 3 cut(s) 152, 653, 1224
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 9 cut(s) 280, 638, 926, 1100, 1176, 1349, 1495, 1610, 1667
HspAI GCGC 2 cut(s) 248, 1262
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 27
KspAI GTTAAC 2 cut(s) 678, 1048
Kzo9I GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
LmnI GCTCC 7 cut(s) 35, 185, 259, 597, 891, 1150, 1610
Lsp1109I GCAGC 4 cut(s) 67, 274, 597, 844
LweI GCATC 1 cut(s) 529
MaeI CTAG 4 cut(s) 770, 1265, 1422, 1533
MaeII ACGT 3 cut(s) 152, 653, 1224
MaeIII GTNAC 4 cut(s) 52, 167, 668, 814
MalI GATC 6 cut(s) 29, 197, 1169, 1428, 1444, 1468
MbiI CCGCTC 1 cut(s) 38
MboI GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
MboII GAAGA 9 cut(s) 22, 355, 406, 473, 737, 1198, 1295, 1420, 1530
MflI RGATCY 2 cut(s) 195, 1167
MhlI GDGCHC 4 cut(s) 108, 300, 954, 1155
MlyI GAGTC 3 cut(s) 263, 1139, 1420
MmeI TCCRAC 6 cut(s) 135, 237, 315, 1245, 1444, 1675
Mph1103I ATGCAT 1 cut(s) 636
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 3 cut(s) 524, 677, 1047
MslI CAYNNNNRTG 1 cut(s) 1344
MspA1I CMGCKG 2 cut(s) 665, 855
MspCI CTTAAG 1 cut(s) 523
MspI CCGG 7 cut(s) 48, 172, 218, 335, 709, 720, 836
MspR9I CCNGG 5 cut(s) 193, 218, 472, 720, 789
MvaI CCWGG 3 cut(s) 193, 472, 789
MwoI GCNNNNNNNGC 6 cut(s) 44, 626, 883, 1060, 1268, 1574
NaeI GCCGGC 1 cut(s) 49
NciI CCSGG 2 cut(s) 218, 720
NcoI CCATGG 2 cut(s) 1172, 1606
NdeI CATATG 1 cut(s) 1453
NdeII GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 9 cut(s) 280, 638, 926, 1100, 1176, 1349, 1495, 1610, 1667
NlaIV GGNNCC 5 cut(s) 88, 197, 286, 333, 1072
NmuCI GTSAC 4 cut(s) 52, 167, 668, 814
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 1667
PaeR7I CTCGAG 1 cut(s) 251
PciI ACATGT 1 cut(s) 1663
PcsI WCGNNNNNNNCGW 2 cut(s) 263, 288
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 8 cut(s) 144, 796, 863, 964, 974, 1349, 1369, 1517
PfoI TCCNGGA 1 cut(s) 470
PkrI GCNGC 7 cut(s) 61, 82, 140, 264, 612, 834, 857
PleI GAGTC 3 cut(s) 262, 1139, 1419
PpsI GAGTC 3 cut(s) 262, 1139, 1419
PpuMI RGGWCCY 1 cut(s) 285
PscI ACATGT 1 cut(s) 1663
Psp124BI GAGCTC 2 cut(s) 108, 1155
Psp1406I AACGTT 1 cut(s) 1224
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 191, 470, 787
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 3 cut(s) 191, 470, 787
PspN4I GGNNCC 5 cut(s) 88, 197, 286, 333, 1072
PspPI GGNCC 4 cut(s) 87, 285, 346, 876
PspPPI RGGWCCY 1 cut(s) 285
PspXI VCTCGAGB 1 cut(s) 251
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PsuI RGATCY 2 cut(s) 195, 1167
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 4 cut(s) 74, 707, 749, 1389
RsaNI GTAC 4 cut(s) 73, 706, 748, 1388
RseI CAYNNNNRTG 1 cut(s) 1344
SacI GAGCTC 2 cut(s) 108, 1155
SaqAI TTAA 3 cut(s) 524, 677, 1047
SatI GCNGC 7 cut(s) 60, 81, 139, 263, 611, 833, 856
Sau3AI GATC 6 cut(s) 27, 195, 1167, 1426, 1442, 1466
Sau96I GGNCC 4 cut(s) 87, 285, 346, 876
SchI GAGTC 3 cut(s) 263, 1139, 1420
ScrFI CCNGG 5 cut(s) 193, 218, 472, 720, 789
SduI GDGCHC 4 cut(s) 108, 300, 954, 1155
SfaNI GCATC 1 cut(s) 529
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 3 cut(s) 87, 285, 346
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 1344
SmlI CTYRAG 4 cut(s) 251, 523, 751, 1618
SmoI CTYRAG 4 cut(s) 251, 523, 751, 1618
SpeI ACTAGT 1 cut(s) 1532
SsiI CCGC 9 cut(s) 38, 59, 123, 138, 165, 349, 492, 855, 1473
SspMI CTAG 4 cut(s) 770, 1265, 1422, 1533
SstI GAGCTC 2 cut(s) 108, 1155
StyD4I CCNGG 5 cut(s) 191, 216, 470, 718, 787
StyI CCWWGG 4 cut(s) 90, 1172, 1398, 1606
TaaI ACNGT 3 cut(s) 307, 937, 1312
TaiI ACGT 3 cut(s) 155, 656, 1227
TaqI TCGA 4 cut(s) 102, 252, 962, 1025
TatI WGTACW 2 cut(s) 72, 1387
TauI GCSGC 3 cut(s) 62, 141, 858
TfiI GAWTC 8 cut(s) 144, 796, 863, 964, 974, 1349, 1369, 1517
Tru1I TTAA 3 cut(s) 524, 677, 1047
Tru9I TTAA 3 cut(s) 524, 677, 1047
TscAI CASTG 3 cut(s) 82, 514, 1531
TseFI GTSAC 4 cut(s) 52, 167, 668, 814
TseI GCWGC 4 cut(s) 80, 262, 610, 832
Tsp45I GTSAC 4 cut(s) 52, 167, 668, 814
TspDTI ATGAA 4 cut(s) 212, 423, 876, 1362
TspRI CASTG 3 cut(s) 82, 514, 1531
Vha464I CTTAAG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 87, 285, 346
XapI RAATTY 4 cut(s) 437, 822, 1381, 1557
XceI RCATGY 1 cut(s) 1667
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XhoI CTCGAG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 4 cut(s) 770, 1265, 1422, 1533
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.