MD16G1255500.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012410
Physical Location & Seq
Reverse (-)
29375310 .. 29376182
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1255500.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGACGGGTCCTCCGACGAGCCCACCACCGTCGCGAAATGTGGGTTGGAGCTGGAACCGGATGTGGTGGACCCCATTTCGTCTCTGTTCAAGAACTTGATGGATTATGCTCAGGGGAAGCAGGTGTTTGATGAAAGTCCAGAGAGAGAGTGCAATGGTGATTTTGAATACAGTAAGCTCGGAGATGACGCTTCCAGGATTTGTGCAAGCAATGACGGTGTTGGGAAGCTCAGTGTCAGTGGCTATACAAATTGGAGCAGAAAGTTGCTGCAAGAGGCCATCTTTGCAATGCATGAGAACATCCAAAGGACTTATACATCAGCCGGTGATTTGTTAACTCTCGATTACAATGCAGAAAGCGAGTACCGGATGTTTCCCGGCGAAGAAATCATCATTGCTAAAGGGTACTTGAGCATTGTTCAATCTCTAGCCTCTGTACTCCCACCTGGGTTGATCCAATTGGGCAAGAAGGTCAGAAAAATTCAATGGCAGCCGGATAATCGCAAGAACAAGGGCTATGAATCCGACACAAGGCTTGTGAAGCTCCATTTTTCTGATGGGTCAGTTTTGTTAGCTGATCATGTTATTGTTACAGTTTCTTTGGGGGTGCTCAAAGCTTCGATTTGCCAAGATTCTGGTATGTTCAATCCACCTCTGCCTCGTTTCAACACCGAGGCGATTCCGAGGCTTGGATTTGGGGTTGTTAACAAGCTGTTTCTGCAACTGGGTTCTATTCATGCTCCTCCGGCATTTGATGCAATTCCCAGGAACCCAAACGCCGTCCGCTGGGCTCTTCCGCTTTAG

Protein Analysis

268

Amino Acids

29.44

Weight (kDa)

5.32

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amino_oxidase PF01593 111 - 242 2.9e-22 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 112
Acc36I ACCTGC 1 cut(s) 112
AccII CGCG 1 cut(s) 35
AciI CCGC 2 cut(s) 784, 797
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 480
AfaI GTAC 3 cut(s) 365, 407, 438
AfiI CCNNNNNNNGG 3 cut(s) 531, 689, 786
AgsI TTSAA 6 cut(s) 91, 167, 422, 485, 646, 667
AjnI CCWGG 3 cut(s) 194, 445, 764
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 7 cut(s) 52, 178, 229, 544, 575, 617, 712
AluI AGCT 7 cut(s) 52, 178, 229, 544, 575, 617, 712
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 448
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 268, 490
ApoI RAATTY 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 9, 70
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 170, 338
AvaII GGWCC 2 cut(s) 9, 70
BanII GRGCYC 2 cut(s) 24, 793
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 255, 502
BccI CCATC 3 cut(s) 94, 287, 551
BceAI ACGGC 1 cut(s) 764
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 3 cut(s) 196, 447, 766
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 87
BfaI CTAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 112
BisI GCNGC 2 cut(s) 269, 491
BlsI GCNGC 2 cut(s) 270, 492
Bme1390I CCNGG 4 cut(s) 196, 378, 447, 766
Bme18I GGWCC 2 cut(s) 9, 70
BmgT120I GGNCC 2 cut(s) 9, 70
BmiI GGNNCC 4 cut(s) 10, 57, 72, 770
BmrFI CCNGG 4 cut(s) 196, 378, 447, 766
BmrI ACTGGG 1 cut(s) 734
BmsI GCATC 1 cut(s) 745
BmuI ACTGGG 1 cut(s) 734
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 430
BpuMI CCSGG 1 cut(s) 378
BsaJI CCNNGG 4 cut(s) 446, 672, 683, 764
BsaWI WCCGGW 2 cut(s) 58, 366
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 531, 689, 786
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 729
Bse3DI GCAATG 4 cut(s) 160, 217, 294, 393
BseBI CCWGG 3 cut(s) 196, 447, 766
BseDI CCNNGG 4 cut(s) 446, 672, 683, 764
BseGI GGATG 3 cut(s) 67, 300, 375
BseLI CCNNNNNNNGG 3 cut(s) 531, 689, 786
BseMI GCAATG 4 cut(s) 160, 217, 294, 393
BseMII CTCAG 2 cut(s) 125, 244
BseNI ACTGG 1 cut(s) 729
BseRI GAGGAG 1 cut(s) 732
BseXI GCAGC 2 cut(s) 255, 502
BseYI CCCAGC 1 cut(s) 786
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 6 cut(s) 59, 324, 367, 378, 494, 746
BslI CCNNNNNNNGG 3 cut(s) 531, 689, 786
BsmAI GTCTC 1 cut(s) 87
BsmBI CGTCTC 1 cut(s) 87
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 24, 612, 793
Bsp143I GATC 2 cut(s) 453, 577
Bsp68I TCGCGA 1 cut(s) 35
BspACI CCGC 2 cut(s) 784, 797
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 124, 243
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 4 cut(s) 10, 57, 72, 770
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 1 cut(s) 448
BspQI GCTCTTC 1 cut(s) 798
BsrDI GCAATG 4 cut(s) 160, 217, 294, 393
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 1 cut(s) 729
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 446, 672, 683, 764
BssMI GATC 2 cut(s) 453, 577
Bst2UI CCWGG 3 cut(s) 196, 447, 766
Bst4CI ACNGT 4 cut(s) 31, 173, 218, 595
Bst6I CTCTTC 1 cut(s) 798
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 111, 230
BstF5I GGATG 3 cut(s) 67, 300, 375
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 2 cut(s) 456, 580
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 2 cut(s) 453, 577
BstMWI GCNNNNNNNGC 5 cut(s) 284, 541, 718, 746, 755
BstNI CCWGG 3 cut(s) 196, 447, 766
BstSCI CCNGG 4 cut(s) 194, 376, 445, 764
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 255, 502
BstXI CCANNNNNNTGG 1 cut(s) 635
BsuRI GGCC 1 cut(s) 278
BtsCI GGATG 3 cut(s) 67, 300, 375
BtsIMutI CAGTG 2 cut(s) 238, 244
BtuMI TCGCGA 1 cut(s) 35
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 9, 70
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 364, 406, 437
CspCI CAANNNNNGTGG 2 cut(s) 432, 467
CviAII CATG 3 cut(s) 293, 581, 737
CviQI GTAC 3 cut(s) 364, 406, 437
DdeI CTNAG 2 cut(s) 111, 230
DpnI GATC 2 cut(s) 455, 579
DpnII GATC 2 cut(s) 453, 577
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Eco24I GRGCYC 2 cut(s) 24, 793
Eco47I GGWCC 2 cut(s) 9, 70
EcoO109I RGGNCCY 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 194, 445, 764
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 24, 793
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 3 cut(s) 296, 584, 740
FaiI YATR 8 cut(s) 108, 246, 294, 315, 519, 582, 641, 738
FatI CATG 3 cut(s) 292, 580, 736
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 269, 491
FokI GGATG 3 cut(s) 74, 287, 382
FriOI GRGCYC 2 cut(s) 24, 793
Fsp4HI GCNGC 2 cut(s) 269, 491
FspBI CTAG 1 cut(s) 428
GluI GCNGC 2 cut(s) 269, 491
GsaI CCCAGC 1 cut(s) 790
HaeIII GGCC 1 cut(s) 278
HapII CCGG 6 cut(s) 59, 324, 367, 378, 494, 746
HgaI GACGC 1 cut(s) 197
Hin1II CATG 3 cut(s) 296, 584, 740
HincII GTYRAC 2 cut(s) 336, 706
HindII GTYRAC 2 cut(s) 336, 706
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 3 cut(s) 521, 632, 679
HpaI GTTAAC 2 cut(s) 336, 706
HpaII CCGG 6 cut(s) 59, 324, 367, 378, 494, 746
HphI GGTGA 2 cut(s) 170, 338
Hpy166II GTNNAC 3 cut(s) 70, 336, 706
Hpy188I TCNGA 6 cut(s) 16, 182, 476, 526, 556, 684
Hpy188III TCNNGA 4 cut(s) 34, 91, 140, 341
Hpy8I GTNNAC 3 cut(s) 70, 336, 706
Hpy99I CGWCG 2 cut(s) 20, 35
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 4 cut(s) 31, 173, 218, 595
HpyCH4V TGCA 8 cut(s) 153, 206, 271, 287, 292, 353, 721, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 284, 541, 718, 746, 755
HpyF3I CTNAG 2 cut(s) 111, 230
Hsp92II CATG 3 cut(s) 296, 584, 740
Ksp22I TGATCA 1 cut(s) 577
KspAI GTTAAC 2 cut(s) 336, 706
Kzo9I GATC 2 cut(s) 453, 577
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 4 cut(s) 49, 255, 549, 745
Lsp1109I GCAGC 2 cut(s) 255, 502
LweI GCATC 1 cut(s) 745
MaeI CTAG 1 cut(s) 428
MaeIII GTNAC 1 cut(s) 589
MalI GATC 2 cut(s) 455, 579
MboI GATC 2 cut(s) 453, 577
MboII GAAGA 2 cut(s) 395, 785
MfeI CAATTG 1 cut(s) 458
MhlI GDGCHC 3 cut(s) 24, 612, 793
MluCI AATT 4 cut(s) 250, 458, 480, 759
MmeI TCCRAC 3 cut(s) 27, 39, 549
MnlI CCTC 8 cut(s) 22, 268, 442, 663, 667, 669, 678, 753
Mph1103I ATGCAT 1 cut(s) 294
MseI TTAA 2 cut(s) 335, 705
MspA1I CMGCKG 1 cut(s) 786
MspI CCGG 6 cut(s) 59, 324, 367, 378, 494, 746
MspR9I CCNGG 4 cut(s) 196, 378, 447, 766
MunI CAATTG 1 cut(s) 458
MvaI CCWGG 3 cut(s) 196, 447, 766
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 5 cut(s) 284, 541, 718, 746, 755
NciI CCSGG 1 cut(s) 378
NdeII GATC 2 cut(s) 453, 577
NlaIII CATG 3 cut(s) 296, 584, 740
NlaIV GGNNCC 4 cut(s) 10, 57, 72, 770
NruI TCGCGA 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 294
PaqCI CACCTGC 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 3 cut(s) 521, 632, 679
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 2 cut(s) 270, 492
PpuMI RGGWCCY 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 3 cut(s) 194, 445, 764
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 3 cut(s) 194, 445, 764
PspN4I GGNNCC 4 cut(s) 10, 57, 72, 770
PspPI GGNCC 2 cut(s) 9, 70
PspPPI RGGWCCY 1 cut(s) 9
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 3 cut(s) 365, 407, 438
RsaNI GTAC 3 cut(s) 364, 406, 437
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 2 cut(s) 335, 705
SatI GCNGC 2 cut(s) 269, 491
Sau3AI GATC 2 cut(s) 453, 577
Sau96I GGNCC 2 cut(s) 9, 70
ScrFI CCNGG 4 cut(s) 196, 378, 447, 766
SduI GDGCHC 3 cut(s) 24, 612, 793
SfaNI GCATC 1 cut(s) 745
SinI GGWCC 2 cut(s) 9, 70
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 4 cut(s) 250, 458, 480, 759
SsiI CCGC 2 cut(s) 784, 797
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 4 cut(s) 194, 376, 445, 764
TaaI ACNGT 4 cut(s) 31, 173, 218, 595
TaqI TCGA 2 cut(s) 342, 620
TasI AATT 4 cut(s) 250, 458, 480, 759
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 3 cut(s) 521, 632, 679
Tru1I TTAA 2 cut(s) 335, 705
Tru9I TTAA 2 cut(s) 335, 705
TscAI CASTG 2 cut(s) 238, 244
TseI GCWGC 2 cut(s) 268, 490
TspDTI ATGAA 3 cut(s) 147, 534, 725
TspRI CASTG 2 cut(s) 238, 244
VpaK11BI GGWCC 2 cut(s) 9, 70
XapI RAATTY 1 cut(s) 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.