MD15G1206900.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012410
Physical Location & Seq
Reverse (-)
16460347 .. 16462697
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1206900.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGGTGGCCAAGAAATCACGGATTGTGATAATTGGAGCGGGAATGGCCGGCGTCACGGCGGCGAACAAGCTGTACACTGCTGCAGGGTCCAAGGACTTGTTCGAGCTCGTGGTTGTGGAAGGCGGGGAGAGAATTGGCGGCAGAATCAACACGTCGGAGTTCGGCGGCGATCGGATTGAGATGGGAGCTACCTGGATCCACGGCATTGGAGGCAGCCCGGTTCATAAAATTGCTCAGGAAATCAATGCCCTCGAGTCAGCGCATCCGTGGGAGTGCATGGACGGGTCCTCCGACGAGCCCACCACCGTCGCGGAAGGTGGGTTGGAGCTGGAACCGGATGTGGTGGACCCCATTTCGTCTCTGTTCAAGAACTTGATGGATTATGCTCAGGGGAAGCAGGTGTTTGATGAAAGTCCAGAGAGAGAGTGCAATGGTGATTTTGAATACAGTAAGCTTGGAGATGAAGCTTCCAGGATTTGTGCAAGCAATGACGATGTTGGGAAGCTCAGTGTCGGCTCTTTCTTAAGGCAAGGCCTTGATACGTATTGGGTTTCGAAGAAAAACCGAGATGAGCAGGTCAGTGTCTATACAAATTGGAGCCGAATGTTGCTGCAAGAGGCCATCTTTGCAATGCATGAGAACATCCAAAGGACTTATACATCAGCCGGTGATTTGTTAACTCTCGATTACAATGCAGAAAGCGAGTACCGGATGTTTCCCGGCGAAGAAATCACCATTGCTAAAGGGTACTTGAGCATTGTTCAATCTCTAGCCTCTGTACTCCCACCTGGGTTGATCCAATTGGGCAAGAAGGTCAGAAAAATTCAATGGCAGCCGGATAATCGCAAGAACAAGGGCTATGAATCCGACACAAGGCCTGTGAAGCTCCATTTTTCTGATGGGTCAGTTTTGTTAGCTGATCATGTTATTGTTACAGTTTCTTTGGGGGTGCTCAAGGCTTCGATTCGCCAAGATTCTGGTATGTTCAATCCACCTCTGCCTCGTTTCAAAACCGAGGCGATTTCGAGGCTTGGATTTGGGGTTGTTAACAAGCTGTTTCTGCAACTGGGTTCTATTCATGCTCCTCCGAAGTCCCAAGATTTCAGCAAGTTTCCATTTTTACAAATGGTTTTCCACAGAGCAGACTCAGAGCTCAGGAATAAAAAGATCCCATGGTGGATGAGGAAGACAGCTTCTCTCTGCCCTCTCTACCACGATTCCAGCGTTCTGCTTTCTTGGTTTGCAGGGGAAGAAGCACGTGCGCTAGAAGCACTTTCAGATGAAGAGATTATCAACGGGGTTTCACAAACGGTGTCCAGCTTCCTATCACAAAACTCACATTCCCATGAATTGTGCAATGGGAATGGGAATCCGGAGGAGAACTCTGAAGTGAAATTTTCCAAGGTTTTGAAGAGTCAATGGGGTAGTGATCCACTGTTTCTGGGATCATACTCGTATGTTGCGGTTGGATCAAGCGGTGAGGATTTGGACAGCATGGCAGAGCCATTGCCATCTGATGATTCTGGTAGTGCTGCTAGTTCTTCACCTCCACTGCAGATTCTGTTTGCAGGGGAAGCTACACACAGAACCCATTATTCCACAACCCATGGAGCTTACTGTAGTGGCCTTAGGGAAGCCAATAGGCTTCTCCAACATTACCAACTTGTTGGTGTTCAGAAGAATTATTACCTGTATTCGGATTATACAAATCAAGGATGA

Protein Analysis

573

Amino Acids

63.0

Weight (kDa)

5.41

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 10 - 75 9.7e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 15 - 550 1.3e-66 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 388, 565
AccBSI CCGCTC 1 cut(s) 38
AccII CGCG 1 cut(s) 311
AccIII TCCGGA 1 cut(s) 1372
AciI CCGC 8 cut(s) 38, 59, 123, 138, 165, 311, 1463, 1476
AclWI GGATC 7 cut(s) 190, 203, 790, 1162, 1424, 1453, 1477
AcoI YGGCCR 2 cut(s) 6, 45
AcsI RAATTY 2 cut(s) 822, 1394
AcuI CTGAAG 1 cut(s) 1407
AcvI CACGTG 1 cut(s) 1259
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 4 cut(s) 74, 707, 749, 780
AfiI CCNNNNNNNGG 2 cut(s) 873, 1696
AflII CTTAAG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 7 cut(s) 367, 443, 764, 827, 988, 1009, 1411
AjiI CACGTC 1 cut(s) 153
AjnI CCWGG 3 cut(s) 191, 470, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
Alw21I GWGCWC 3 cut(s) 108, 954, 1155
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 7 cut(s) 190, 203, 790, 1162, 1424, 1453, 1477
AlwNI CAGNNNCTG 1 cut(s) 1561
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 1 cut(s) 1372
AoxI GGCC 6 cut(s) 6, 45, 532, 618, 875, 1624
ApeKI GCWGC 5 cut(s) 80, 213, 610, 832, 1532
ApoI RAATTY 2 cut(s) 822, 1394
AspLEI GCGC 2 cut(s) 262, 1264
AspS9I GGNCC 3 cut(s) 87, 285, 346
AsuC2I CCSGG 2 cut(s) 218, 720
AsuHPI GGTGA 5 cut(s) 446, 680, 724, 1490, 1536
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 87, 285, 346
AxyI CCTNAGG 1 cut(s) 1628
BalI TGGCCA 1 cut(s) 8
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 3 cut(s) 108, 300, 1155
BarI GAAGNNNNNNTAC 2 cut(s) 1670, 1702
BauI CACGAG 1 cut(s) 107
BbrPI CACGTG 1 cut(s) 1259
BbsI GAAGAC 1 cut(s) 1193
Bbv12I GWGCWC 3 cut(s) 108, 954, 1155
BbvI GCAGC 5 cut(s) 67, 225, 597, 844, 1519
BccI CCATC 5 cut(s) 175, 370, 629, 893, 1519
BceAI ACGGC 2 cut(s) 72, 217
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 3 cut(s) 193, 472, 789
BclI TGATCA 1 cut(s) 919
BcnI CCSGG 2 cut(s) 218, 720
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 3 cut(s) 770, 1265, 1536
BfmI CTRYAG 3 cut(s) 81, 1553, 1618
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 2 cut(s) 388, 565
BisI GCNGC 8 cut(s) 60, 81, 139, 166, 214, 611, 833, 1533
BlsI GCNGC 8 cut(s) 61, 82, 140, 167, 215, 612, 834, 1534
Bme1390I CCNGG 5 cut(s) 193, 218, 472, 720, 789
Bme18I GGWCC 3 cut(s) 87, 285, 346
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 87, 285, 346
BmiI GGNNCC 6 cut(s) 88, 197, 286, 333, 348, 599
BmrFI CCNGG 5 cut(s) 193, 218, 472, 720, 789
BmrI ACTGGG 1 cut(s) 1076
BmsI GCATC 1 cut(s) 271
BmuI ACTGGG 1 cut(s) 1076
BpiI GAAGAC 1 cut(s) 1193
BplI GAGNNNNNCTC 2 cut(s) 1367, 1399
Bpu10I CCTNAGC 3 cut(s) 234, 387, 1154
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 2 cut(s) 772, 938
BpuMI CCSGG 2 cut(s) 218, 720
BsaAI YACGTR 2 cut(s) 543, 1259
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1401, 1606
BsaWI WCCGGW 3 cut(s) 334, 708, 1372
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 873, 1696
Bse118I RCCGGY 2 cut(s) 47, 665
Bse1I ACTGG 1 cut(s) 1071
Bse21I CCTNAGG 1 cut(s) 1628
Bse3DI GCAATG 6 cut(s) 436, 493, 636, 735, 1363, 1505
BseAI TCCGGA 1 cut(s) 1372
BseBI CCWGG 3 cut(s) 193, 472, 789
BseDI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1401, 1606
BseGI GGATG 5 cut(s) 262, 343, 642, 717, 1185
BseLI CCNNNNNNNGG 2 cut(s) 873, 1696
BseMI GCAATG 6 cut(s) 436, 493, 636, 735, 1363, 1505
BseMII CTCAG 5 cut(s) 248, 401, 520, 1161, 1168
BseNI ACTGG 1 cut(s) 1071
BseRI GAGGAG 2 cut(s) 1074, 1391
BseXI GCAGC 5 cut(s) 67, 225, 597, 844, 1519
Bsh1236I CGCG 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 6 cut(s) 8, 47, 534, 620, 877, 1626
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 3 cut(s) 108, 954, 1155
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 8 cut(s) 48, 218, 335, 666, 709, 720, 836, 1373
BslFI GGGAC 1 cut(s) 1078
BslI CCNNNNNNNGG 2 cut(s) 873, 1696
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 1078
BsnI GGCC 6 cut(s) 8, 47, 534, 620, 877, 1626
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 4 cut(s) 108, 300, 954, 1155
Bsp13I TCCGGA 1 cut(s) 1372
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
Bsp19I CCATGG 2 cut(s) 1172, 1606
BspACI CCGC 8 cut(s) 38, 59, 123, 138, 165, 311, 1463, 1476
BspANI GGCC 6 cut(s) 8, 47, 534, 620, 877, 1626
BspCNI CTCAG 5 cut(s) 247, 400, 519, 1160, 1167
BspEI TCCGGA 1 cut(s) 1372
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 6 cut(s) 88, 197, 286, 333, 348, 599
BspMAI CTGCAG 2 cut(s) 85, 1557
BspMI ACCTGC 2 cut(s) 388, 565
BspPI GGATC 7 cut(s) 190, 203, 790, 1162, 1424, 1453, 1477
BspT104I TTCGAA 1 cut(s) 554
BspTI CTTAAG 1 cut(s) 523
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 6 cut(s) 436, 493, 636, 735, 1363, 1505
BsrFI RCCGGY 2 cut(s) 47, 665
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 1071
BssAI RCCGGY 2 cut(s) 47, 665
BssECI CCNNGG 8 cut(s) 90, 199, 266, 788, 1014, 1172, 1401, 1606
BssMI GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 4 cut(s) 90, 1172, 1401, 1606
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 3 cut(s) 193, 472, 789
Bst4CI ACNGT 6 cut(s) 307, 449, 937, 1312, 1437, 1619
Bst6I CTCTTC 2 cut(s) 1278, 1406
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 523
BstAUI TGTACA 1 cut(s) 72
BstBAI YACGTR 2 cut(s) 543, 1259
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 49, 484
BstDEI CTNAG 6 cut(s) 234, 387, 506, 1147, 1154, 1628
BstDSI CCRYGG 4 cut(s) 199, 266, 1172, 1606
BstF5I GGATG 5 cut(s) 262, 343, 642, 717, 1185
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 2 cut(s) 262, 1264
BstKTI GATC 8 cut(s) 172, 198, 798, 922, 1170, 1432, 1448, 1472
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 7 cut(s) 44, 210, 626, 883, 1060, 1268, 1574
BstNI CCWGG 3 cut(s) 193, 472, 789
BstSCI CCNGG 5 cut(s) 191, 216, 470, 718, 787
BstSFI CTRYAG 3 cut(s) 81, 1553, 1618
BstSNI TACGTA 1 cut(s) 543
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 5 cut(s) 67, 225, 597, 844, 1519
BstV2I GAAGAC 1 cut(s) 1193
BstX2I RGATCY 2 cut(s) 195, 1167
BstXI CCANNNNNNTGG 3 cut(s) 206, 977, 1667
BstYI RGATCY 2 cut(s) 195, 1167
Bsu36I CCTNAGG 1 cut(s) 1628
BsuRI GGCC 6 cut(s) 8, 47, 534, 620, 877, 1626
BtgI CCRYGG 4 cut(s) 199, 266, 1172, 1606
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 5 cut(s) 262, 343, 642, 717, 1185
BtsI GCAGTG 2 cut(s) 75, 1550
BtsIMutI CAGTG 5 cut(s) 75, 514, 586, 1433, 1550
BveI ACCTGC 2 cut(s) 388, 565
Cac8I GCNNGC 2 cut(s) 49, 484
CaiI CAGNNNCTG 1 cut(s) 1561
CfoI GCGC 2 cut(s) 262, 1264
Cfr10I RCCGGY 2 cut(s) 47, 665
Cfr13I GGNCC 3 cut(s) 87, 285, 346
CseI GACGC 1 cut(s) 40
Csp6I GTAC 4 cut(s) 73, 706, 748, 779
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviAII CATG 8 cut(s) 277, 635, 923, 1079, 1173, 1346, 1495, 1607
CviQI GTAC 4 cut(s) 73, 706, 748, 779
DdeI CTNAG 6 cut(s) 234, 387, 506, 1147, 1154, 1628
DpnI GATC 8 cut(s) 171, 197, 797, 921, 1169, 1431, 1447, 1471
DpnII GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
EaeI YGGCCR 2 cut(s) 6, 45
Eam1104I CTCTTC 2 cut(s) 1278, 1406
EarI CTCTTC 2 cut(s) 1278, 1406
Ecl136II GAGCTC 2 cut(s) 106, 1153
Eco105I TACGTA 1 cut(s) 543
Eco130I CCWWGG 4 cut(s) 90, 1172, 1401, 1606
Eco147I AGGCCT 2 cut(s) 534, 877
Eco24I GRGCYC 3 cut(s) 108, 300, 1155
Eco47I GGWCC 3 cut(s) 87, 285, 346
Eco53kI GAGCTC 2 cut(s) 106, 1153
Eco57I CTGAAG 1 cut(s) 1407
Eco72I CACGTG 1 cut(s) 1259
Eco81I CCTNAGG 1 cut(s) 1628
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 2 cut(s) 106, 1153
EcoO109I RGGNCCY 1 cut(s) 285
EcoRII CCWGG 3 cut(s) 191, 470, 787
EcoT14I CCWWGG 4 cut(s) 90, 1172, 1401, 1606
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 3 cut(s) 108, 300, 1155
ErhI CCWWGG 4 cut(s) 90, 1172, 1401, 1606
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 8 cut(s) 280, 638, 926, 1082, 1176, 1349, 1498, 1610
FaqI GGGAC 1 cut(s) 1078
FatI CATG 8 cut(s) 276, 634, 922, 1078, 1172, 1345, 1494, 1606
FauI CCCGC 2 cut(s) 31, 116
FbaI TGATCA 1 cut(s) 919
Fnu4HI GCNGC 8 cut(s) 60, 81, 139, 166, 214, 611, 833, 1533
FokI GGATG 5 cut(s) 249, 350, 629, 724, 1192
FriOI GRGCYC 3 cut(s) 108, 300, 1155
Fsp4HI GCNGC 8 cut(s) 60, 81, 139, 166, 214, 611, 833, 1533
FspBI CTAG 3 cut(s) 770, 1265, 1536
GlaI GCGC 2 cut(s) 261, 1263
GluI GCNGC 8 cut(s) 60, 81, 139, 166, 214, 611, 833, 1533
HaeIII GGCC 6 cut(s) 8, 47, 534, 620, 877, 1626
HapII CCGG 8 cut(s) 48, 218, 335, 666, 709, 720, 836, 1373
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 262, 1264
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 8 cut(s) 280, 638, 926, 1082, 1176, 1349, 1498, 1610
Hin6I GCGC 2 cut(s) 260, 1262
HinP1I GCGC 2 cut(s) 260, 1262
HincII GTYRAC 2 cut(s) 678, 1048
HindII GTYRAC 2 cut(s) 678, 1048
HindIII AAGCTT 2 cut(s) 452, 465
HpaI GTTAAC 2 cut(s) 678, 1048
HpaII CCGG 8 cut(s) 48, 218, 335, 666, 709, 720, 836, 1373
HphI GGTGA 5 cut(s) 446, 680, 724, 1490, 1536
Hpy166II GTNNAC 4 cut(s) 75, 346, 678, 1048
Hpy188III TCNNGA 6 cut(s) 236, 367, 416, 683, 1156, 1373
Hpy8I GTNNAC 4 cut(s) 75, 346, 678, 1048
Hpy99I CGWCG 3 cut(s) 157, 296, 311
HpyAV CCTTC 3 cut(s) 113, 308, 805
HpyCH4III ACNGT 6 cut(s) 307, 449, 937, 1312, 1437, 1619
HpyCH4IV ACGT 3 cut(s) 152, 542, 1258
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 210, 626, 883, 1060, 1268, 1574
HpyF3I CTNAG 6 cut(s) 234, 387, 506, 1147, 1154, 1628
HpySE526I ACGT 3 cut(s) 152, 542, 1258
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 8 cut(s) 280, 638, 926, 1082, 1176, 1349, 1498, 1610
HspAI GCGC 2 cut(s) 260, 1262
Kpn2I TCCGGA 1 cut(s) 1372
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 919
KspAI GTTAAC 2 cut(s) 678, 1048
Kzo9I GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
LmnI GCTCC 7 cut(s) 35, 185, 325, 597, 891, 1087, 1610
Lsp1109I GCAGC 5 cut(s) 67, 225, 597, 844, 1519
LweI GCATC 1 cut(s) 271
MaeI CTAG 3 cut(s) 770, 1265, 1536
MaeII ACGT 3 cut(s) 152, 542, 1258
MaeIII GTNAC 2 cut(s) 52, 931
MalI GATC 8 cut(s) 171, 197, 797, 921, 1169, 1431, 1447, 1471
MbiI CCGCTC 1 cut(s) 38
MboI GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
MboII GAAGA 8 cut(s) 568, 737, 1198, 1262, 1295, 1423, 1533, 1690
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 2 cut(s) 195, 1167
MhlI GDGCHC 4 cut(s) 108, 300, 954, 1155
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 9 cut(s) 30, 132, 228, 592, 800, 822, 1349, 1394, 1681
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 3 cut(s) 263, 1139, 1423
MmeI TCCRAC 6 cut(s) 135, 303, 315, 891, 1447, 1675
Mox20I TGGCCA 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 636
MroI TCCGGA 1 cut(s) 1372
MroNI GCCGGC 1 cut(s) 47
MscI TGGCCA 1 cut(s) 8
MseI TTAA 3 cut(s) 524, 677, 1047
MslI CAYNNNNRTG 1 cut(s) 1344
Msp20I TGGCCA 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 523
MspI CCGG 8 cut(s) 48, 218, 335, 666, 709, 720, 836, 1373
MspR9I CCNGG 5 cut(s) 193, 218, 472, 720, 789
MunI CAATTG 1 cut(s) 800
MvaI CCWGG 3 cut(s) 193, 472, 789
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 7 cut(s) 44, 210, 626, 883, 1060, 1268, 1574
NaeI GCCGGC 1 cut(s) 49
NciI CCSGG 2 cut(s) 218, 720
NcoI CCATGG 2 cut(s) 1172, 1606
NdeII GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 8 cut(s) 280, 638, 926, 1082, 1176, 1349, 1498, 1610
NlaIV GGNNCC 6 cut(s) 88, 197, 286, 333, 348, 599
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 636
NspV TTCGAA 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 388
PceI AGGCCT 2 cut(s) 534, 877
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 1221
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 8 cut(s) 144, 863, 964, 974, 1217, 1369, 1520, 1558
PfoI TCCNGGA 1 cut(s) 470
PkrI GCNGC 8 cut(s) 61, 82, 140, 167, 215, 612, 834, 1534
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 3 cut(s) 262, 1139, 1422
PmaCI CACGTG 1 cut(s) 1259
PmlI CACGTG 1 cut(s) 1259
PpsI GAGTC 3 cut(s) 262, 1139, 1422
Ppu21I YACGTR 2 cut(s) 543, 1259
PpuMI RGGWCCY 1 cut(s) 285
Psp124BI GAGCTC 2 cut(s) 108, 1155
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 191, 470, 787
PspCI CACGTG 1 cut(s) 1259
PspGI CCWGG 3 cut(s) 191, 470, 787
PspN4I GGNNCC 6 cut(s) 88, 197, 286, 333, 348, 599
PspPI GGNCC 3 cut(s) 87, 285, 346
PspPPI RGGWCCY 1 cut(s) 285
PspXI VCTCGAGB 1 cut(s) 251
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 2 cut(s) 85, 1557
PstNI CAGNNNCTG 1 cut(s) 1561
PsuI RGATCY 2 cut(s) 195, 1167
PvuI CGATCG 1 cut(s) 172
RsaI GTAC 4 cut(s) 74, 707, 749, 780
RsaNI GTAC 4 cut(s) 73, 706, 748, 779
RseI CAYNNNNRTG 1 cut(s) 1344
SacI GAGCTC 2 cut(s) 108, 1155
SaqAI TTAA 3 cut(s) 524, 677, 1047
SatI GCNGC 8 cut(s) 60, 81, 139, 166, 214, 611, 833, 1533
Sau3AI GATC 8 cut(s) 169, 195, 795, 919, 1167, 1429, 1445, 1469
Sau96I GGNCC 3 cut(s) 87, 285, 346
SchI GAGTC 3 cut(s) 263, 1139, 1423
ScrFI CCNGG 5 cut(s) 193, 218, 472, 720, 789
SduI GDGCHC 4 cut(s) 108, 300, 954, 1155
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 3 cut(s) 81, 1553, 1618
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 3 cut(s) 87, 285, 346
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 1344
SmlI CTYRAG 4 cut(s) 251, 523, 751, 953
SmoI CTYRAG 4 cut(s) 251, 523, 751, 953
SnaBI TACGTA 1 cut(s) 543
Sse9I AATT 9 cut(s) 30, 132, 228, 592, 800, 822, 1349, 1394, 1681
SseBI AGGCCT 2 cut(s) 534, 877
SsiI CCGC 8 cut(s) 38, 59, 123, 138, 165, 311, 1463, 1476
SspMI CTAG 3 cut(s) 770, 1265, 1536
SstI GAGCTC 2 cut(s) 108, 1155
StuI AGGCCT 2 cut(s) 534, 877
StyD4I CCNGG 5 cut(s) 191, 216, 470, 718, 787
StyI CCWWGG 4 cut(s) 90, 1172, 1401, 1606
TaaI ACNGT 6 cut(s) 307, 449, 937, 1312, 1437, 1619
TaiI ACGT 3 cut(s) 155, 545, 1261
TaqI TCGA 6 cut(s) 102, 252, 554, 684, 962, 1025
TasI AATT 9 cut(s) 30, 132, 228, 592, 800, 822, 1349, 1394, 1681
TatI WGTACW 2 cut(s) 72, 778
TauI GCSGC 3 cut(s) 62, 141, 168
TfiI GAWTC 8 cut(s) 144, 863, 964, 974, 1217, 1369, 1520, 1558
Tru1I TTAA 3 cut(s) 524, 677, 1047
Tru9I TTAA 3 cut(s) 524, 677, 1047
TscAI CASTG 5 cut(s) 82, 514, 586, 1440, 1557
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 5 cut(s) 80, 213, 610, 832, 1532
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 7 cut(s) 212, 423, 477, 876, 1067, 1296, 1362
TspGWI ACGGA 2 cut(s) 34, 255
TspRI CASTG 5 cut(s) 82, 514, 586, 1440, 1557
Vha464I CTTAAG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 87, 285, 346
XapI RAATTY 2 cut(s) 822, 1394
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XhoI CTCGAG 1 cut(s) 251
XspI CTAG 3 cut(s) 770, 1265, 1536
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.