AT4G29780

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015894
Physical Location & Seq
Forward (+)
14579563 .. 14582057
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29780.1

Sequence Viewer

Length: 1623 bp
ATGGAAATCTCTTCTTTCCCATTTCCATACCTACAAGACGACGAGTGTTCCCATTTCCTTGGTCTATTTCAAGACATGGACTCTTCTCCTTCTACTTTCGGATTAGAAGGTTTTAATAGCAACGACAATAATACTAATCAAAAGAAACGCCCAAGAAAAGACGACGAAGGCGGTGGTGGTGGCGGCGGAGGAACAGAAGTTCTAGGAGCTGTTAATGGTAATAATAAGGCTGCTTTTGGAGATATACTCGCGACGCTTCTGTTGTTAGACGAGGAAGCTAAACAGCAACAAGAACAGTGGGATTTTGAATTTATTAAAGAGAAGTCTTTACTTGAAGCTAATCATAAGAAGAAAGTGAAAACAATGGATGGTTATTACAATCAAATGCAAGATCATTACTCTGCAGCTGGTGAAACCGATGGTTCGCGTTCAAAACGCGCACGGAAAACCGCGGTTGCGGCTGTGGTTTCCGCGGTAGCTTCCGGGGCGGACACAACCGGTTTAGCTGCTCCGGTTCCGACCGCGGATATCGCTAGCGGTTCCGGGTCAGGACCGAGTCATAGGAGGTTATGGGTTAAAGAACGAACCACGGACTGGTGGGACAGAGTAAGCCGGCCTGATTTTCCAGAAGACGAGTTTCGGCGAGAGTTCCGTATGAGCAAATCGACGTTTAACCTAATATGCGAGGAGCTAGATACGACGGTGACGAAGAAAAACACGATGTTAAGAGACGCGATTCCAGCTCCAAAACGCGTAGGCGTTTGCGTTTGGCGTTTGGCGACAGGAGCTCCGCTTCGCCACGTGTCGGAGCGTTTCGGTCTGGGAATCTCAACTTGCCACAAACTAGTCATCGAAGTCTGCCGCGCGATCTACGACGTTCTCATGCCCAAGTATCTCCTCTGGCCGTCGGATTCAGAGATAAACTCAACGAAAGCCAAATTCGAATCGGTCCACAAAATACCAAACGTCGTCGGATCAATCTACACCACACATATTCCGATCATCGCTCCGAAAGTCCACGTGGCGGCGTATTTTAACAAGAGACACACGGAGAGGAATCAGAAGACGTCGTACTCGATAACAGTACAAGGAGTGGTCAACGCCGACGGGATCTTCACCGACGTTTGTATCGGAAACCCAGGATCTCTCACCGACGATCAGATCCTGGAGAAATCTTCGCTTTCACGGCAAAGAGCGGCGCGTGGGATGTTACGTGACAGCTGGATAGTTGGAAACTCTGGGTTTCCGTTGACTGATTATCTTCTTGTACCGTACACGAGACAGAATCTGACGTGGACGCAGCACGCGTTTAACGAGAGTATCGGAGAGATTCAGGGGATTGCGACGGCTGCGTTTGAGAGGCTCAAAGGACGGTGGGCTTGTTTGCAGAAACGGACGGAGGTGAAGCTTCAGGATCTGCCGTACGTGCTTGGAGCTTGTTGTGTGTTGCATAACATTTGTGAGATGAGGAAGGAGGAGATGTTGCCGGAGTTGAAGTTTGAGGTTTTTGATGATGTGGCGGTGCCGGAGAATAATATCCGATCTGCTAGTGCGGTTAATACGAGGGATCATATCTCTCACAATCTCTTGCATCGTGGACTTGCCGGGACAAGAACTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

540

Amino Acids

60.12

Weight (kDa)

6.95

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 327 - 485 2.3e-36 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1070
AccB1I GGYRCC 1 cut(s) 1522
AccB7I CCANNNNNTGG 1 cut(s) 594
AccBSI CCGCTC 1 cut(s) 1196
AclWI GGATC 6 cut(s) 982, 1118, 1150, 1156, 1422, 1575
AcoI YGGCCR 1 cut(s) 902
AcsI RAATTY 2 cut(s) 308, 938
AcuI CTGAAG 1 cut(s) 1394
AcvI CACGTG 2 cut(s) 802, 1021
AcyI GRCGYC 1 cut(s) 1067
AfaI GTAC 5 cut(s) 1073, 1086, 1269, 1274, 1424
AfiI CCNNNNNNNGG 3 cut(s) 594, 805, 1024
AflIII ACRYGT 3 cut(s) 751, 801, 1305
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 5 cut(s) 71, 308, 335, 432, 1495
AhlI ACTAGT 1 cut(s) 844
AjiI CACGTC 1 cut(s) 1293
AjnI CCWGG 2 cut(s) 1138, 1164
AloI GAACNNNNNNTCC 2 cut(s) 183, 215
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 3 cut(s) 723, 1036, 1273
AlwI GGATC 6 cut(s) 982, 1118, 1150, 1156, 1422, 1575
AlwNI CAGNNNCTG 3 cut(s) 1165, 1288, 1417
AoxI GGCC 2 cut(s) 614, 902
ApeKI GCWGC 5 cut(s) 230, 404, 506, 1300, 1349
ApoI RAATTY 2 cut(s) 308, 938
AsiGI ACCGGT 1 cut(s) 497
AspLEI GCGC 3 cut(s) 440, 866, 1201
AspS9I GGNCC 2 cut(s) 551, 949
AsuC2I CCSGG 3 cut(s) 484, 544, 1606
AsuHPI GGTGA 5 cut(s) 422, 715, 1108, 1141, 1414
AsuII TTCGAA 1 cut(s) 942
AsuNHI GCTAGC 1 cut(s) 533
AvaII GGWCC 2 cut(s) 551, 949
BaeI ACNNNNGTAYC 6 cut(s) 687, 720, 1111, 1144, 1303, 1336
BanI GGYRCC 1 cut(s) 1522
BanII GRGCYC 1 cut(s) 790
BarI GAAGNNNNNNTAC 2 cut(s) 1055, 1087
BauI CACGAG 1 cut(s) 1276
BbrPI CACGTG 2 cut(s) 802, 1021
BbsI GAAGAC 2 cut(s) 636, 1070
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 5 cut(s) 217, 416, 493, 1312, 1336
BccI CCATC 2 cut(s) 362, 413
BceAI ACGGC 4 cut(s) 889, 1202, 1362, 1405
BciT130I CCWGG 2 cut(s) 1140, 1166
BcnI CCSGG 3 cut(s) 484, 544, 1606
BcoDI GTCTC 3 cut(s) 723, 1036, 1273
BcuI ACTAGT 1 cut(s) 844
BfaI CTAG 5 cut(s) 203, 534, 692, 845, 1548
BfmI CTRYAG 2 cut(s) 402, 1619
Bme1390I CCNGG 5 cut(s) 484, 544, 1140, 1166, 1606
Bme18I GGWCC 2 cut(s) 551, 949
BmgBI CACGTC 1 cut(s) 1293
BmgT120I GGNCC 2 cut(s) 551, 949
BmiI GGNNCC 3 cut(s) 516, 541, 1524
BmrFI CCNGG 5 cut(s) 484, 544, 1140, 1166, 1606
BmsI GCATC 1 cut(s) 1600
BmtI GCTAGC 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 636, 1070
BplI GAGNNNNNCTC 4 cut(s) 231, 263, 908, 940
BpmI CTGGAG 1 cut(s) 1187
Bpu14I TTCGAA 1 cut(s) 942
BpuMI CCSGG 3 cut(s) 484, 544, 1606
BsaAI YACGTR 4 cut(s) 802, 1021, 1214, 1426
BsaHI GRCGYC 1 cut(s) 1067
BsaJI CCNNGG 7 cut(s) 58, 450, 471, 483, 522, 588, 1138
BsaWI WCCGGW 2 cut(s) 497, 511
BsaXI ACNNNNNCTCC 6 cut(s) 772, 802, 1466, 1481, 1496, 1511
Bsc4I CCNNNNNNNGG 3 cut(s) 594, 805, 1024
Bse118I RCCGGY 2 cut(s) 497, 612
Bse1I ACTGG 1 cut(s) 599
BseBI CCWGG 2 cut(s) 1140, 1166
BseDI CCNNGG 7 cut(s) 58, 450, 471, 483, 522, 588, 1138
BseGI GGATG 2 cut(s) 373, 1212
BseLI CCNNNNNNNGG 3 cut(s) 594, 805, 1024
BseNI ACTGG 1 cut(s) 599
BseRI GAGGAG 3 cut(s) 701, 887, 1490
BseXI GCAGC 5 cut(s) 217, 416, 493, 1312, 1336
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 2 cut(s) 616, 904
BshNI GGYRCC 1 cut(s) 1522
BshTI ACCGGT 1 cut(s) 497
BsiEI CGRYCG 1 cut(s) 522
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 8 cut(s) 483, 498, 512, 543, 613, 1487, 1526, 1605
BsiWI CGTACG 1 cut(s) 1422
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 3 cut(s) 594, 805, 1024
BsmAI GTCTC 3 cut(s) 723, 1036, 1273
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 1 cut(s) 614
BsnI GGCC 2 cut(s) 616, 904
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 1 cut(s) 790
Bsp68I TCGCGA 1 cut(s) 251
BspANI GGCC 2 cut(s) 616, 904
BspLI GGNNCC 3 cut(s) 516, 541, 1524
BspMAI CTGCAG 1 cut(s) 406
BspOI GCTAGC 1 cut(s) 537
BspPI GGATC 6 cut(s) 982, 1118, 1150, 1156, 1422, 1575
BspT104I TTCGAA 1 cut(s) 942
BspT107I GGYRCC 1 cut(s) 1522
BsrBI CCGCTC 1 cut(s) 1196
BsrFI RCCGGY 2 cut(s) 497, 612
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 2 cut(s) 497, 612
BssECI CCNNGG 7 cut(s) 58, 450, 471, 483, 522, 588, 1138
BssNI GRCGYC 1 cut(s) 1067
BssSI CACGAG 1 cut(s) 1276
BssT1I CCWWGG 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 1276
Bst2UI CCWGG 2 cut(s) 1140, 1166
Bst4CI ACNGT 5 cut(s) 297, 703, 1084, 1272, 1374
Bst6I CTCTTC 2 cut(s) 16, 88
BstACI GRCGYC 1 cut(s) 1067
BstBAI YACGTR 4 cut(s) 802, 1021, 1214, 1426
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 3 cut(s) 535, 614, 1305
BstDSI CCRYGG 4 cut(s) 450, 471, 522, 588
BstF5I GGATG 2 cut(s) 373, 1212
BstHHI GCGC 3 cut(s) 440, 866, 1201
BstMAI GTCTC 3 cut(s) 723, 1036, 1273
BstMCI CGRYCG 1 cut(s) 522
BstMWI GCNNNNNNNGC 8 cut(s) 458, 485, 530, 740, 785, 1186, 1349, 1426
BstNI CCWGG 2 cut(s) 1140, 1166
BstSCI CCNGG 5 cut(s) 482, 542, 1138, 1164, 1604
BstSFI CTRYAG 2 cut(s) 402, 1619
BstV1I GCAGC 5 cut(s) 217, 416, 493, 1312, 1336
BstV2I GAAGAC 2 cut(s) 636, 1070
BstX2I RGATCY 4 cut(s) 1110, 1142, 1161, 1414
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 4 cut(s) 1110, 1142, 1161, 1414
BsuRI GGCC 2 cut(s) 616, 904
BtgI CCRYGG 4 cut(s) 450, 471, 522, 588
BtgZI GCGATG 1 cut(s) 988
BtrI CACGTC 1 cut(s) 1293
BtsCI GGATG 2 cut(s) 373, 1212
BtsIMutI CAGTG 1 cut(s) 302
BtuMI TCGCGA 1 cut(s) 251
Cac8I GCNNGC 3 cut(s) 535, 614, 1305
CaiI CAGNNNCTG 3 cut(s) 1165, 1288, 1417
CfoI GCGC 3 cut(s) 440, 866, 1201
Cfr10I RCCGGY 2 cut(s) 497, 612
Cfr13I GGNCC 2 cut(s) 551, 949
Cfr42I CCGCGG 3 cut(s) 453, 474, 525
CseI GACGC 3 cut(s) 262, 740, 1306
Csp6I GTAC 5 cut(s) 1072, 1085, 1268, 1273, 1423
CspAI ACCGGT 1 cut(s) 497
CspCI CAANNNNNGTGG 2 cut(s) 278, 313
CviAII CATG 2 cut(s) 76, 883
CviQI GTAC 5 cut(s) 1072, 1085, 1268, 1273, 1423
EaeI YGGCCR 1 cut(s) 902
Eam1104I CTCTTC 2 cut(s) 16, 88
EarI CTCTTC 2 cut(s) 16, 88
EciI GGCGGA 2 cut(s) 201, 503
Ecl136II GAGCTC 1 cut(s) 788
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 790
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 551, 949
Eco53kI GAGCTC 1 cut(s) 788
Eco57I CTGAAG 1 cut(s) 1394
Eco72I CACGTG 2 cut(s) 802, 1021
EcoICRI GAGCTC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 1138, 1164
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 1 cut(s) 790
ErhI CCWWGG 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 2 cut(s) 79, 886
FaqI GGGAC 1 cut(s) 614
FatI CATG 2 cut(s) 75, 882
FokI GGATG 2 cut(s) 380, 1219
FriOI GRGCYC 1 cut(s) 790
FspBI CTAG 5 cut(s) 203, 534, 692, 845, 1548
GlaI GCGC 3 cut(s) 439, 865, 1200
GsuI CTGGAG 1 cut(s) 1187
HaeIII GGCC 2 cut(s) 616, 904
HapII CCGG 8 cut(s) 483, 498, 512, 543, 613, 1487, 1526, 1605
HgaI GACGC 3 cut(s) 262, 740, 1306
HhaI GCGC 3 cut(s) 440, 866, 1201
Hin1I GRCGYC 1 cut(s) 1067
Hin1II CATG 2 cut(s) 79, 886
Hin6I GCGC 3 cut(s) 438, 864, 1199
HinP1I GCGC 3 cut(s) 438, 864, 1199
HincII GTYRAC 2 cut(s) 1099, 1251
HindII GTYRAC 2 cut(s) 1099, 1251
HindIII AAGCTT 1 cut(s) 1406
HinfI GANTC 9 cut(s) 80, 556, 736, 825, 911, 944, 1057, 1285, 1330
HpaII CCGG 8 cut(s) 483, 498, 512, 543, 613, 1487, 1526, 1605
HphI GGTGA 5 cut(s) 422, 715, 1108, 1141, 1414
Hpy166II GTNNAC 7 cut(s) 952, 1018, 1099, 1251, 1275, 1296, 1598
Hpy188III TCNNGA 5 cut(s) 71, 250, 549, 626, 1412
Hpy8I GTNNAC 7 cut(s) 952, 1018, 1099, 1251, 1275, 1296, 1598
HpyAV CCTTC 4 cut(s) 99, 101, 161, 1465
HpyCH4III ACNGT 5 cut(s) 297, 703, 1084, 1272, 1374
HpyCH4V TGCA 5 cut(s) 388, 404, 1387, 1450, 1591
HpyF10VI GCNNNNNNNGC 8 cut(s) 458, 485, 530, 740, 785, 1186, 1349, 1426
Hsp92I GRCGYC 1 cut(s) 1067
Hsp92II CATG 2 cut(s) 79, 886
HspAI GCGC 3 cut(s) 438, 864, 1199
KroI GCCGGC 1 cut(s) 612
KroNI GCCGGC 1 cut(s) 614
KspI CCGCGG 3 cut(s) 453, 474, 525
LmnI GCTCC 9 cut(s) 206, 514, 688, 748, 785, 793, 808, 1012, 1433
Lsp1109I GCAGC 5 cut(s) 217, 416, 493, 1312, 1336
LweI GCATC 1 cut(s) 1600
MaeI CTAG 5 cut(s) 203, 534, 692, 845, 1548
MaeIII GTNAC 3 cut(s) 703, 1209, 1214
MbiI CCGCTC 1 cut(s) 1196
MboII GAAGA 9 cut(s) 3, 75, 361, 641, 721, 1075, 1105, 1167, 1253
MflI RGATCY 4 cut(s) 1110, 1142, 1161, 1414
MhlI GDGCHC 1 cut(s) 790
MluCI AATT 2 cut(s) 308, 938
MluI ACGCGT 2 cut(s) 751, 1305
MlyI GAGTC 2 cut(s) 74, 565
MmeI TCCRAC 5 cut(s) 542, 786, 888, 952, 1210
MroNI GCCGGC 1 cut(s) 612
MseI TTAA 9 cut(s) 114, 213, 315, 576, 672, 725, 1035, 1311, 1557
MspA1I CMGCKG 5 cut(s) 407, 452, 473, 524, 1221
MspI CCGG 8 cut(s) 483, 498, 512, 543, 613, 1487, 1526, 1605
MspR9I CCNGG 5 cut(s) 484, 544, 1140, 1166, 1606
MvaI CCWGG 2 cut(s) 1140, 1166
MwoI GCNNNNNNNGC 8 cut(s) 458, 485, 530, 740, 785, 1186, 1349, 1426
NaeI GCCGGC 1 cut(s) 614
NciI CCSGG 3 cut(s) 484, 544, 1606
NgoMIV GCCGGC 1 cut(s) 612
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 2 cut(s) 79, 886
NlaIV GGNNCC 3 cut(s) 516, 541, 1524
NmuCI GTSAC 2 cut(s) 703, 1214
NruI TCGCGA 1 cut(s) 251
NspV TTCGAA 1 cut(s) 942
PcsI WCGNNNNNNNCGW 4 cut(s) 704, 1073, 1304, 1311
PdiI GCCGGC 1 cut(s) 614
PfeI GAWTC 7 cut(s) 736, 825, 911, 944, 1057, 1285, 1330
Pfl23II CGTACG 1 cut(s) 1422
PflFI GACNNNGTC 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 594
PfoI TCCNGGA 1 cut(s) 1164
PinAI ACCGGT 1 cut(s) 497
PleI GAGTC 2 cut(s) 74, 564
PmaCI CACGTG 2 cut(s) 802, 1021
PmlI CACGTG 2 cut(s) 802, 1021
PpsI GAGTC 2 cut(s) 74, 564
Ppu21I YACGTR 4 cut(s) 802, 1021, 1214, 1426
Psp124BI GAGCTC 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 1138, 1164
PspCI CACGTG 2 cut(s) 802, 1021
PspGI CCWGG 2 cut(s) 1138, 1164
PspLI CGTACG 1 cut(s) 1422
PspN4I GGNNCC 3 cut(s) 516, 541, 1524
PspPI GGNCC 2 cut(s) 551, 949
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 3 cut(s) 1165, 1288, 1417
PsuI RGATCY 4 cut(s) 1110, 1142, 1161, 1414
PsyI GACNNNGTC 1 cut(s) 555
PvuII CAGCTG 2 cut(s) 407, 1221
RruI TCGCGA 1 cut(s) 251
RsaI GTAC 5 cut(s) 1073, 1086, 1269, 1274, 1424
RsaNI GTAC 5 cut(s) 1072, 1085, 1268, 1273, 1423
SacI GAGCTC 1 cut(s) 790
SacII CCGCGG 3 cut(s) 453, 474, 525
SaqAI TTAA 9 cut(s) 114, 213, 315, 576, 672, 725, 1035, 1311, 1557
Sau96I GGNCC 2 cut(s) 551, 949
SchI GAGTC 2 cut(s) 74, 565
ScrFI CCNGG 5 cut(s) 484, 544, 1140, 1166, 1606
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 1 cut(s) 1600
SfcI CTRYAG 2 cut(s) 402, 1619
Sfr303I CCGCGG 3 cut(s) 453, 474, 525
SfuI TTCGAA 1 cut(s) 942
SgrBI CCGCGG 3 cut(s) 453, 474, 525
SinI GGWCC 2 cut(s) 551, 949
SpeI ACTAGT 1 cut(s) 844
Sse9I AATT 2 cut(s) 308, 938
SspMI CTAG 5 cut(s) 203, 534, 692, 845, 1548
SstI GAGCTC 1 cut(s) 790
StyD4I CCNGG 5 cut(s) 482, 542, 1138, 1164, 1604
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 5 cut(s) 297, 703, 1084, 1272, 1374
TaqI TCGA 4 cut(s) 665, 852, 942, 1076
TaqII GACCGA 3 cut(s) 568, 806, 937
TasI AATT 2 cut(s) 308, 938
TatI WGTACW 1 cut(s) 1084
TauI GCSGC 5 cut(s) 186, 461, 864, 1028, 1199
TfiI GAWTC 7 cut(s) 736, 825, 911, 944, 1057, 1285, 1330
Tru1I TTAA 9 cut(s) 114, 213, 315, 576, 672, 725, 1035, 1311, 1557
Tru9I TTAA 9 cut(s) 114, 213, 315, 576, 672, 725, 1035, 1311, 1557
TscAI CASTG 1 cut(s) 302
TseFI GTSAC 2 cut(s) 703, 1214
TseI GCWGC 5 cut(s) 230, 404, 506, 1300, 1349
Tsp45I GTSAC 2 cut(s) 703, 1214
TspGWI ACGGA 7 cut(s) 457, 605, 641, 1064, 1236, 1408, 1412
TspRI CASTG 1 cut(s) 302
Tth111I GACNNNGTC 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 594
VpaK11BI GGWCC 2 cut(s) 551, 949
XapI RAATTY 2 cut(s) 308, 938
XspI CTAG 5 cut(s) 203, 534, 692, 845, 1548
ZraI GACGTC 1 cut(s) 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.