RLG00000016446

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015894
Physical Location & Seq
Forward (+)
6696423 .. 6697988
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016446

Sequence Viewer

Length: 1566 bp
ATGGAAGTCACCCCTTTCACATTTCTTAATCAAGAGGAGTACTTGAACTTCTTCAGCTTATATCCCGAGATGCAAGACTTCGGAATGAGTGACACTACTGCTATTGGGTTGAAGAAGCGGAGGAAGGTCGAGGAGGACGATACCAATGGCGCCGATGAAGAAATGAAGAAGAAGATTCCTTTCACTGACATTCTCACATCGTTGATGATATTGGACGAGGAAGAGAAGCAGGAGCAACAACACTACTTTGCGCAATCCCAGCTACAAAAGGACAGTCTTGACGCCAATTACAGGAAGAAATCCGAAGTGATGGATCAGTATTATTCTGATTTCCAGCAGAGCGGTTCTGAATTGAACAGCTTGGAGCAGTCCCGCGGCAAAAGGAGCCGCGGCGCTGCTTTTGCCGCGGCTGCTACCGCGGTTGCCGCTGCCGCCGAAACTACTACTACTACTGCTGTTCTACCGGCGGCGGCCACCGCGGAGACCTCCGCTGCGGCAGGGCCGCACCGGAGGCTGTGGGTTAAGGACAGGTCCAAGGACTGGTGGGACCACTACAACAGCCCCAACGTTTCTGAGGACGAGTTTCGGAAGGCGTTTCGGATGAGCAAGGGCACATTCGACATGATTTGCGAGGAGCTGGACTCCGCCGTGACCAAGAAGAACACAATGCTGAGGGATGCGATTCCGGTGCGGCAGCGGGTGGCCGTCTGCATATGGAGGCTGGGTACCGGCGAGCCGCTCCGTCTCGTTTCGAAACGGTTCGGTCTCGGCATCTCCACCTGCCACAAGCTTGTGCTTGAAGTCTGCCTGGCTATCAAGACTGTTCTAATGGAGAAGTATCTCCACTGGCCCGATGAGCACACATTGCAAGCGATCAAAACCCAGTTCGAATCCATTTCCGGCATTCCTAATGTCTCTGGTTCTATGTACACTACTCATGTTCCGATCATAGCACCCAAAGCGAGTGTAGCAGCCTATTTCAACAAGCGCCACACCGAACGTAACCAGAAGACTTCTTACTCCATCACAGTTCAAGGTGTGGTTGATCCTAGAGGCTTGTTTACCGACATTTGCGTCGGCTGGCCTGGTTCTATGCCGGACGACAAGGTGTTGGAAAATTCTGCTCTGTACAAAAGAGGCAGCTCCGGCCAATTGAAGGACGTTTGGGTGGTCGGAAACAAAGGTCACCCTTTGATGGATTGGGTTTTGGTTCCCTACACCCACCAGAATTTGACATGGACACAACACGCCTTCAATGAAAAGATTGGGGACATTCAAGGAGTTGCTAAAGATGCATTTGCTAAGCTCAAAGGGAGGTGGTCTTGTTTACAGAAGAGGACTGAGGTGAAGCTCCAGGACCTGCCTGTGGTGCTTGGGGCTTGCTGTGTTCTCCACAACATTTGCACCATGAGGAACGATGAGCTTGATCCCGAGCTCAAATACGATCTCTTTGATGATGAGATGGTTTCTGAGAATGGTCTGAGGTCTGCTGGTGCAGTTCAAGCTAGGGATCACATTGCTCATAACTTGCTTCACCATGGCCTTGCAGGCACTGGATTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

522

Amino Acids

58.62

Weight (kDa)

6.3

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 192 - 272 9.9e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 310 - 466 3.2e-34 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 788
AasI GACNNNNNNGTC 1 cut(s) 1073
Acc16I TGCGCA 1 cut(s) 252
Acc36I ACCTGC 2 cut(s) 788, 1368
Acc65I GGTACC 1 cut(s) 725
AccB1I GGYRCC 2 cut(s) 149, 725
AccB7I CCANNNNNTGG 1 cut(s) 540
AccBSI CCGCTC 2 cut(s) 342, 739
AccII CGCG 5 cut(s) 375, 390, 407, 419, 479
AclI AACGTT 1 cut(s) 567
AclWI GGATC 4 cut(s) 321, 1040, 1421, 1518
AcoI YGGCCR 3 cut(s) 471, 702, 1147
AcsI RAATTY 2 cut(s) 1117, 1228
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 2 cut(s) 150, 282
AfaI GTAC 4 cut(s) 41, 727, 929, 1130
AfiI CCNNNNNNNGG 5 cut(s) 291, 540, 1156, 1195, 1366
AjnI CCWGG 3 cut(s) 807, 1084, 1353
Alw21I GWGCWC 2 cut(s) 861, 1437
Alw26I GTCTC 4 cut(s) 476, 749, 770, 919
AlwI GGATC 4 cut(s) 321, 1040, 1421, 1518
AlwNI CAGNNNCTG 2 cut(s) 1360, 1553
Ama87I CYCGRG 2 cut(s) 65, 1430
AoxI GGCC 7 cut(s) 471, 500, 702, 848, 1082, 1147, 1540
ApeKI GCWGC 7 cut(s) 395, 410, 428, 491, 694, 971, 1140
ApoI RAATTY 2 cut(s) 1117, 1228
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
Asp700I GAANNNNTTC 2 cut(s) 50, 758
Asp718I GGTACC 1 cut(s) 725
AspLEI GCGC 4 cut(s) 152, 253, 395, 990
AspS9I GGNCC 5 cut(s) 500, 531, 547, 849, 1357
AsuHPI GGTGA 3 cut(s) 1178, 1357, 1526
AsuII TTCGAA 2 cut(s) 752, 888
AvaI CYCGRG 2 cut(s) 65, 1430
AvaII GGWCC 3 cut(s) 531, 547, 1357
BaeGI GKGCMC 1 cut(s) 614
BanI GGYRCC 2 cut(s) 149, 725
BanII GRGCYC 1 cut(s) 1437
BarI GAAGNNNNNNTAC 4 cut(s) 32, 64, 1001, 1033
BbsI GAAGAC 1 cut(s) 1016
Bbv12I GWGCWC 2 cut(s) 861, 1437
BbvCI CCTCAGC 1 cut(s) 671
BbvI GCAGC 7 cut(s) 382, 397, 415, 478, 706, 983, 1152
BccI CCATC 4 cut(s) 304, 1031, 1189, 1456
BceAI ACGGC 2 cut(s) 632, 689
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 3 cut(s) 809, 1086, 1355
BcoDI GTCTC 4 cut(s) 476, 749, 770, 919
BfaI CTAG 2 cut(s) 1050, 1506
BfoI RGCGCY 3 cut(s) 153, 396, 991
BfuAI ACCTGC 2 cut(s) 788, 1368
BglI GCCNNNNNGGC 1 cut(s) 1548
BlpI GCTNAGC 1 cut(s) 1302
BmcAI AGTACT 1 cut(s) 41
Bme1390I CCNGG 3 cut(s) 809, 1086, 1355
Bme18I GGWCC 3 cut(s) 531, 547, 1357
BmeT110I CYCGRG 2 cut(s) 65, 1430
BmgT120I GGNCC 5 cut(s) 500, 531, 547, 849, 1357
BmiI GGNNCC 5 cut(s) 151, 386, 548, 727, 1212
BmrFI CCNGG 3 cut(s) 809, 1086, 1355
BmrI ACTGGG 1 cut(s) 877
BmsI GCATC 4 cut(s) 60, 667, 780, 1282
BmuI ACTGGG 1 cut(s) 877
BpiI GAAGAC 1 cut(s) 1016
BplI GAGNNNNNCTC 2 cut(s) 626, 658
BpmI CTGGAG 1 cut(s) 1337
Bpu10I CCTNAGC 1 cut(s) 671
Bpu1102I GCTNAGC 1 cut(s) 1302
Bpu14I TTCGAA 2 cut(s) 752, 888
BsaHI GRCGYC 2 cut(s) 150, 282
BsaI GGTCTC 2 cut(s) 476, 770
BsaJI CCNNGG 7 cut(s) 373, 388, 405, 417, 477, 534, 1537
BsaWI WCCGGW 2 cut(s) 507, 685
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 540, 1156, 1195, 1366
Bse118I RCCGGY 2 cut(s) 463, 728
Bse1I ACTGG 4 cut(s) 545, 851, 883, 1558
Bse3DI GCAATG 2 cut(s) 863, 1515
BseBI CCWGG 3 cut(s) 809, 1086, 1355
BseDI CCNNGG 7 cut(s) 373, 388, 405, 417, 477, 534, 1537
BseGI GGATG 2 cut(s) 606, 682
BseLI CCNNNNNNNGG 5 cut(s) 291, 540, 1156, 1195, 1366
BseMI GCAATG 2 cut(s) 863, 1515
BseMII CTCAG 5 cut(s) 564, 662, 1332, 1461, 1472
BseNI ACTGG 4 cut(s) 545, 851, 883, 1558
BseRI GAGGAG 3 cut(s) 50, 146, 647
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 7 cut(s) 382, 397, 415, 478, 706, 983, 1152
BseYI CCCAGC 2 cut(s) 258, 721
BsgI GTGCAG 1 cut(s) 1515
Bsh1236I CGCG 5 cut(s) 375, 390, 407, 419, 479
BshFI GGCC 7 cut(s) 473, 502, 704, 850, 1084, 1149, 1542
BshNI GGYRCC 2 cut(s) 149, 725
BsiHKAI GWGCWC 2 cut(s) 861, 1437
BsiHKCI CYCGRG 2 cut(s) 65, 1430
BsiSI CCGG 7 cut(s) 464, 508, 686, 729, 900, 1097, 1146
BslFI GGGAC 3 cut(s) 355, 560, 1283
BslI CCNNNNNNNGG 5 cut(s) 291, 540, 1156, 1195, 1366
BsmAI GTCTC 4 cut(s) 476, 749, 770, 919
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 3 cut(s) 355, 560, 1283
BsmI GAATGC 1 cut(s) 903
BsnI GGCC 7 cut(s) 473, 502, 704, 850, 1084, 1149, 1542
Bso31I GGTCTC 2 cut(s) 476, 770
BsoBI CYCGRG 2 cut(s) 65, 1430
Bsp119I TTCGAA 2 cut(s) 752, 888
Bsp1286I GDGCHC 3 cut(s) 614, 861, 1437
Bsp1407I TGTACA 2 cut(s) 927, 1128
Bsp143I GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
Bsp1720I GCTNAGC 1 cut(s) 1302
Bsp19I CCATGG 1 cut(s) 1537
BspANI GGCC 7 cut(s) 473, 502, 704, 850, 1084, 1149, 1542
BspCNI CTCAG 5 cut(s) 565, 663, 1333, 1462, 1473
BspFNI CGCG 5 cut(s) 375, 390, 407, 419, 479
BspLI GGNNCC 5 cut(s) 151, 386, 548, 727, 1212
BspMI ACCTGC 2 cut(s) 788, 1368
BspPI GGATC 4 cut(s) 321, 1040, 1421, 1518
BspT104I TTCGAA 2 cut(s) 752, 888
BspT107I GGYRCC 2 cut(s) 149, 725
BspTNI GGTCTC 2 cut(s) 476, 770
BsrBI CCGCTC 2 cut(s) 342, 739
BsrDI GCAATG 2 cut(s) 863, 1515
BsrFI RCCGGY 2 cut(s) 463, 728
BsrGI TGTACA 2 cut(s) 927, 1128
BsrI ACTGG 4 cut(s) 545, 851, 883, 1558
BssAI RCCGGY 2 cut(s) 463, 728
BssECI CCNNGG 7 cut(s) 373, 388, 405, 417, 477, 534, 1537
BssMI GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
BssNI GRCGYC 2 cut(s) 150, 282
BssT1I CCWWGG 2 cut(s) 534, 1537
Bst2UI CCWGG 3 cut(s) 809, 1086, 1355
Bst4CI ACNGT 4 cut(s) 275, 759, 823, 1030
Bst6I CTCTTC 2 cut(s) 216, 1328
BstACI GRCGYC 2 cut(s) 150, 282
BstAPI GCANNNNNTGC 1 cut(s) 865
BstAUI TGTACA 2 cut(s) 927, 1128
BstBI TTCGAA 2 cut(s) 752, 888
BstC8I GCNNGC 5 cut(s) 734, 870, 1082, 1381, 1549
BstDEI CTNAG 6 cut(s) 573, 671, 1302, 1341, 1470, 1481
BstDSI CCRYGG 6 cut(s) 373, 388, 405, 417, 477, 1537
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 2 cut(s) 606, 682
BstFNI CGCG 5 cut(s) 375, 390, 407, 419, 479
BstH2I RGCGCY 3 cut(s) 153, 396, 991
BstHHI GCGC 4 cut(s) 152, 253, 395, 990
BstKTI GATC 7 cut(s) 316, 876, 948, 1048, 1429, 1447, 1513
BstMAI GTCTC 4 cut(s) 476, 749, 770, 919
BstMBI GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
BstNI CCWGG 3 cut(s) 809, 1086, 1355
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 3 cut(s) 807, 1084, 1353
BstSLI GKGCMC 1 cut(s) 614
BstUI CGCG 5 cut(s) 375, 390, 407, 419, 479
BstV1I GCAGC 7 cut(s) 382, 397, 415, 478, 706, 983, 1152
BstV2I GAAGAC 1 cut(s) 1016
BsuRI GGCC 7 cut(s) 473, 502, 704, 850, 1084, 1149, 1542
BtgI CCRYGG 6 cut(s) 373, 388, 405, 417, 477, 1537
BtsCI GGATG 2 cut(s) 606, 682
BtsIMutI CAGTG 3 cut(s) 183, 844, 1551
BveI ACCTGC 2 cut(s) 788, 1368
Cac8I GCNNGC 5 cut(s) 734, 870, 1082, 1381, 1549
CaiI CAGNNNCTG 2 cut(s) 1360, 1553
CfoI GCGC 4 cut(s) 152, 253, 395, 990
Cfr10I RCCGGY 2 cut(s) 463, 728
Cfr13I GGNCC 5 cut(s) 500, 531, 547, 849, 1357
Cfr42I CCGCGG 5 cut(s) 376, 391, 408, 420, 480
CseI GACGC 2 cut(s) 290, 1063
Csp6I GTAC 4 cut(s) 40, 726, 928, 1129
CspCI CAANNNNNGTGG 2 cut(s) 1382, 1417
CviAII CATG 5 cut(s) 622, 938, 1236, 1408, 1538
CviQI GTAC 4 cut(s) 40, 726, 928, 1129
DdeI CTNAG 6 cut(s) 573, 671, 1302, 1341, 1470, 1481
DinI GGCGCC 1 cut(s) 151
DpnI GATC 7 cut(s) 315, 875, 947, 1047, 1428, 1446, 1512
DpnII GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
DrdI GACNNNNNNGTC 1 cut(s) 1073
DseDI GACNNNNNNGTC 1 cut(s) 1073
EaeI YGGCCR 3 cut(s) 471, 702, 1147
Eam1104I CTCTTC 2 cut(s) 216, 1328
EarI CTCTTC 2 cut(s) 216, 1328
EciI GGCGGA 1 cut(s) 634
Ecl136II GAGCTC 1 cut(s) 1435
Eco130I CCWWGG 2 cut(s) 534, 1537
Eco24I GRGCYC 1 cut(s) 1437
Eco31I GGTCTC 2 cut(s) 476, 770
Eco47I GGWCC 3 cut(s) 531, 547, 1357
Eco53kI GAGCTC 1 cut(s) 1435
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 2 cut(s) 65, 1430
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 1435
EcoO109I RGGNCCY 1 cut(s) 1357
EcoO65I GGTNACC 1 cut(s) 1184
EcoRII CCWGG 3 cut(s) 807, 1084, 1353
EcoT14I CCWWGG 2 cut(s) 534, 1537
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 1437
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 2 cut(s) 534, 1537
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 5 cut(s) 625, 941, 1239, 1411, 1541
FaqI GGGAC 3 cut(s) 355, 560, 1283
FatI CATG 5 cut(s) 621, 937, 1235, 1407, 1537
FauI CCCGC 2 cut(s) 380, 690
FauNDI CATATG 1 cut(s) 713
FokI GGATG 2 cut(s) 613, 689
FriOI GRGCYC 1 cut(s) 1437
FspBI CTAG 2 cut(s) 1050, 1506
FspI TGCGCA 1 cut(s) 252
GlaI GCGC 4 cut(s) 151, 252, 394, 989
GsaI CCCAGC 2 cut(s) 262, 725
GsuI CTGGAG 1 cut(s) 1337
HaeII RGCGCY 3 cut(s) 153, 396, 991
HaeIII GGCC 7 cut(s) 473, 502, 704, 850, 1084, 1149, 1542
HapII CCGG 7 cut(s) 464, 508, 686, 729, 900, 1097, 1146
HgaI GACGC 2 cut(s) 290, 1063
HhaI GCGC 4 cut(s) 152, 253, 395, 990
Hin1I GRCGYC 2 cut(s) 150, 282
Hin1II CATG 5 cut(s) 625, 941, 1239, 1411, 1541
Hin6I GCGC 4 cut(s) 150, 251, 393, 988
HinP1I GCGC 4 cut(s) 150, 251, 393, 988
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 4 cut(s) 175, 641, 682, 890
HpaII CCGG 7 cut(s) 464, 508, 686, 729, 900, 1097, 1146
HphI GGTGA 3 cut(s) 1178, 1357, 1526
Hpy166II GTNNAC 3 cut(s) 930, 1062, 1328
Hpy188III TCNNGA 5 cut(s) 32, 65, 278, 817, 1430
Hpy8I GTNNAC 3 cut(s) 930, 1062, 1328
Hpy99I CGWCG 1 cut(s) 1079
HpyAV CCTTC 4 cut(s) 118, 583, 1150, 1261
HpyCH4III ACNGT 4 cut(s) 275, 759, 823, 1030
HpyCH4IV ACGT 3 cut(s) 567, 1000, 1161
HpyCH4V TGCA 7 cut(s) 73, 711, 868, 1295, 1404, 1496, 1547
HpyF3I CTNAG 6 cut(s) 573, 671, 1302, 1341, 1470, 1481
HpySE526I ACGT 3 cut(s) 567, 1000, 1161
Hsp92I GRCGYC 2 cut(s) 150, 282
Hsp92II CATG 5 cut(s) 625, 941, 1239, 1411, 1541
HspAI GCGC 4 cut(s) 150, 251, 393, 988
KasI GGCGCC 1 cut(s) 149
KpnI GGTACC 1 cut(s) 729
KspI CCGCGG 5 cut(s) 376, 391, 408, 420, 480
Kzo9I GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
LmnI GCTCC 7 cut(s) 232, 364, 384, 634, 744, 1148, 1356
Lsp1109I GCAGC 7 cut(s) 382, 397, 415, 478, 706, 983, 1152
LweI GCATC 4 cut(s) 60, 667, 780, 1282
MaeI CTAG 2 cut(s) 1050, 1506
MaeII ACGT 3 cut(s) 567, 1000, 1161
MaeIII GTNAC 5 cut(s) 7, 89, 649, 1001, 1184
MalI GATC 7 cut(s) 315, 875, 947, 1047, 1428, 1446, 1512
MbiI CCGCTC 2 cut(s) 342, 739
MboI GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
MfeI CAATTG 1 cut(s) 1151
MhlI GDGCHC 3 cut(s) 614, 861, 1437
MluCI AATT 5 cut(s) 286, 350, 1117, 1151, 1228
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 1 cut(s) 635
MmeI TCCRAC 2 cut(s) 1092, 1153
Mph1103I ATGCAT 1 cut(s) 1297
MroXI GAANNNNTTC 2 cut(s) 50, 758
MseI TTAA 2 cut(s) 27, 522
MspA1I CMGCKG 8 cut(s) 375, 390, 407, 419, 428, 479, 491, 697
MspI CCGG 7 cut(s) 464, 508, 686, 729, 900, 1097, 1146
MspR9I CCNGG 3 cut(s) 809, 1086, 1355
MunI CAATTG 1 cut(s) 1151
Mva1269I GAATGC 1 cut(s) 903
MvaI CCWGG 3 cut(s) 809, 1086, 1355
MvnI CGCG 5 cut(s) 375, 390, 407, 419, 479
NarI GGCGCC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 1537
NdeI CATATG 1 cut(s) 713
NdeII GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
NlaIII CATG 5 cut(s) 625, 941, 1239, 1411, 1541
NlaIV GGNNCC 5 cut(s) 151, 386, 548, 727, 1212
NmeAIII GCCGAG 1 cut(s) 747
NmuCI GTSAC 4 cut(s) 7, 89, 649, 1184
NsbI TGCGCA 1 cut(s) 252
NsiI ATGCAT 1 cut(s) 1297
NspV TTCGAA 2 cut(s) 752, 888
PaqCI CACCTGC 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 903
PdmI GAANNNNTTC 2 cut(s) 50, 758
PfeI GAWTC 3 cut(s) 175, 682, 890
PflMI CCANNNNNTGG 1 cut(s) 540
PfoI TCCNGGA 1 cut(s) 1353
PleI GAGTC 1 cut(s) 635
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 1357
Psp124BI GAGCTC 1 cut(s) 1437
Psp1406I AACGTT 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 1357
Psp6I CCWGG 3 cut(s) 807, 1084, 1353
PspEI GGTNACC 1 cut(s) 1184
PspFI CCCAGC 2 cut(s) 258, 721
PspGI CCWGG 3 cut(s) 807, 1084, 1353
PspN4I GGNNCC 5 cut(s) 151, 386, 548, 727, 1212
PspPI GGNCC 5 cut(s) 500, 531, 547, 849, 1357
PspPPI RGGWCCY 1 cut(s) 1357
PstNI CAGNNNCTG 2 cut(s) 1360, 1553
RsaI GTAC 4 cut(s) 41, 727, 929, 1130
RsaNI GTAC 4 cut(s) 40, 726, 928, 1129
SacI GAGCTC 1 cut(s) 1437
SacII CCGCGG 5 cut(s) 376, 391, 408, 420, 480
SaqAI TTAA 2 cut(s) 27, 522
Sau3AI GATC 7 cut(s) 313, 873, 945, 1045, 1426, 1444, 1510
Sau96I GGNCC 5 cut(s) 500, 531, 547, 849, 1357
ScaI AGTACT 1 cut(s) 41
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 3 cut(s) 809, 1086, 1355
SduI GDGCHC 3 cut(s) 614, 861, 1437
SfaNI GCATC 4 cut(s) 60, 667, 780, 1282
SfoI GGCGCC 1 cut(s) 151
Sfr303I CCGCGG 5 cut(s) 376, 391, 408, 420, 480
SfuI TTCGAA 2 cut(s) 752, 888
SgrBI CCGCGG 5 cut(s) 376, 391, 408, 420, 480
SinI GGWCC 3 cut(s) 531, 547, 1357
Sse9I AATT 5 cut(s) 286, 350, 1117, 1151, 1228
SspDI GGCGCC 1 cut(s) 149
SspMI CTAG 2 cut(s) 1050, 1506
SstI GAGCTC 1 cut(s) 1437
StyD4I CCNGG 3 cut(s) 807, 1084, 1353
StyI CCWWGG 2 cut(s) 534, 1537
TaaI ACNGT 4 cut(s) 275, 759, 823, 1030
TaiI ACGT 3 cut(s) 570, 1003, 1164
TaqI TCGA 4 cut(s) 129, 618, 752, 888
TaqII GACCGA 1 cut(s) 752
TasI AATT 5 cut(s) 286, 350, 1117, 1151, 1228
TatI WGTACW 3 cut(s) 39, 927, 1128
TfiI GAWTC 3 cut(s) 175, 682, 890
Tru1I TTAA 2 cut(s) 27, 522
Tru9I TTAA 2 cut(s) 27, 522
TscAI CASTG 3 cut(s) 190, 851, 1558
TseFI GTSAC 4 cut(s) 7, 89, 649, 1184
TseI GCWGC 7 cut(s) 395, 410, 428, 491, 694, 971, 1140
Tsp45I GTSAC 4 cut(s) 7, 89, 649, 1184
TspDTI ATGAA 4 cut(s) 171, 179, 1272, 1550
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 3 cut(s) 190, 851, 1558
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 3 cut(s) 531, 547, 1357
XapI RAATTY 2 cut(s) 1117, 1228
XmnI GAANNNNTTC 2 cut(s) 50, 758
XspI CTAG 2 cut(s) 1050, 1506
ZrmI AGTACT 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.