Prupe.7G205200_v2.0.a1

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015894
Physical Location & Seq
Reverse (-)
18976509 .. 18978026
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G205200.1

Sequence Viewer

Length: 1518 bp
ATGGAAATTACATCGTTCCCATTTCTTAGTCAAGAAGATTACTCCCATTTCTACACTCTGTTCCCCGACATGGACGAGTGCGACATAAATGGAGAGGGTGGCTTGAAGAAGCGGAGAAGAAAGGAGGACGACAGCAACAACAACAATGGCCCAATGAGCGACATACTCACCACGCTGATTTTGCTGGATGAGGAAGAGAAGCAGGAGCAAGAAGAGTTCTTTGTTCAAACCCAGCAGGAAAAATCGATCCTTGAAGCCAATCACAGGCAGAAGACTCGAGCTATGAATGAGTACTTGTCTGATCTTCAACAGAACCATGCTCAATCTGACGAATTGGACCAGTCTCGCGCGAAACGGACTCACCAATCGGCATTTGCGGCGGCTGCGGCGGTTGCCGAGACTGCCAATTCGAGTGCGGGTTCGGAGTCGGGGTCGGCTGTTCCGGCGGCGGGTCCTCACCGGAGGCTATGGGTCAAGGACAGGAACAAAGATTGGTGGGATAAGTACAACAATCCAAACGTTTCCGACGACGAGTTCCGGGAGGCGTTTCGGATGAGCAAGGCCACATTTGACATGATCTGCGAGGAGCTTGATTCGGCCGTGACGAAGAAGAACACAATGCTGCGCGACGCGATTCCTGTTCGGCAGCGGGTGGCGGTCTGCATATGGCGGTTGGCTACTGGCGAGCCGCTCCGGCTGGTTTCGAAACGTTTCGGGTTGGGCATCTCCACCTGTCACAAGCTGGTTCTTGAGGTTTGCTCTGCTATTAAGACTGTTCTAATGCCCAAGTTTCTTCAGTGGCCTGACGAGAACAGAATGAAAGCTATCAAGGAGGAGTTTGAAGCCAGCTCTGGAATACCAAATGTTGGTGGCTCGATGTACACAACCCATGTTCCAATTATAGCTCCAAAGGTGAATGTTGCAGCTTATTTCAACAAGAGGCACACAGAGCGCAACCAGAAGACCTCTTACTCCATTACAGTGCAAGGAGTTGTTGATCCAAGAGGTGTTTTTACCGATGTTTGTATTGGCTGGCCTGGTTCTATGCCTGATGACCAGGTTTTGGAGAAGTCAGCTTTGTTCCAGAGAGCAAACAGGGGCCTTCTGAAGGATGTTTGGATTGTGGGAAATTCTGGGTTCCCTCTAACAGATTGGGTTTTGGTTCCTTACACCCACCAGAACCTTACTTGGACCCAACATGCCTTCAATGAGAAAATTGGAGATGTTCAGAGGGTTGCTAAAGATGCTTTTGCAAAGTTGAAAGGGAGGTGGTCTTGCTTGCAGAAGAGGACTGAGGTCAAACTACAGGACTTGCCAGTGGTGCTTGGGGCTTGCTGTGTTTTGCACAACATTTGTGCCATGAGGAATGAAGAGCTTGATGCTAATCTAAGTTTTGATATCTTTGATGATGAGATTGTCCCTGAGAATAGTTTGAGGTCTGCTACTTCAGTGCAGGCTAGGGATCACATTGCTCATAATTTGTTGCACCATGGCCTTGCAGGCACTGCTTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

506

Amino Acids

57.07

Weight (kDa)

5.96

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 866, 1063
AccBSI CCGCTC 1 cut(s) 691
AccII CGCG 4 cut(s) 348, 350, 627, 632
AclI AACGTT 2 cut(s) 519, 709
AclWI GGATC 3 cut(s) 241, 992, 1470
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 1 cut(s) 1129
AcuI CTGAAG 3 cut(s) 779, 1127, 1431
AfaI GTAC 3 cut(s) 293, 506, 881
AfiI CCNNNNNNNGG 6 cut(s) 70, 264, 449, 866, 1063, 1108
AgsI TTSAA 8 cut(s) 106, 227, 254, 308, 842, 934, 1207, 1261
AjnI CCWGG 2 cut(s) 1036, 1056
AluBI AGCT 9 cut(s) 281, 589, 742, 824, 849, 905, 926, 1076, 1375
AluI AGCT 9 cut(s) 281, 589, 742, 824, 849, 905, 926, 1076, 1375
Alw26I GTCTC 2 cut(s) 348, 392
AlwI GGATC 3 cut(s) 241, 992, 1470
AlwNI CAGNNNCTG 1 cut(s) 1505
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 7 cut(s) 148, 561, 597, 800, 1034, 1099, 1492
ApeKI GCWGC 4 cut(s) 383, 622, 646, 923
ApoI RAATTY 1 cut(s) 1129
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
Asp700I GAANNNNTTC 1 cut(s) 710
AspLEI GCGC 3 cut(s) 350, 627, 954
AspS9I GGNCC 5 cut(s) 149, 337, 452, 1099, 1191
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 4 cut(s) 160, 353, 449, 925
AsuII TTCGAA 1 cut(s) 704
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 3 cut(s) 337, 452, 1191
BarI GAAGNNNNNNTAC 2 cut(s) 953, 985
BbsI GAAGAC 2 cut(s) 278, 968
BbvI GCAGC 4 cut(s) 370, 609, 658, 935
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 2 cut(s) 1038, 1058
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 2 cut(s) 348, 392
BfaI CTAG 1 cut(s) 1458
BfmI CTRYAG 2 cut(s) 1304, 1514
BglI GCCNNNNNGGC 2 cut(s) 694, 1500
BisI GCNGC 9 cut(s) 378, 381, 384, 387, 447, 623, 647, 689, 924
BlsI GCNGC 9 cut(s) 379, 382, 385, 388, 448, 624, 648, 690, 925
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 3 cut(s) 539, 1038, 1058
Bme18I GGWCC 3 cut(s) 337, 452, 1191
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 5 cut(s) 149, 337, 452, 1099, 1191
BmiI GGNNCC 5 cut(s) 453, 1100, 1139, 1164, 1193
BmrFI CCNGG 3 cut(s) 539, 1038, 1058
BmsI GCATC 3 cut(s) 732, 1234, 1369
BoxI GACNNNNGTC 1 cut(s) 1295
BpiI GAAGAC 2 cut(s) 278, 968
BplI GAGNNNNNCTC 2 cut(s) 743, 775
Bpu14I TTCGAA 1 cut(s) 704
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 1383
BsaJI CCNNGG 1 cut(s) 1489
BsaWI WCCGGW 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 264, 449, 866, 1063, 1108
Bse1I ACTGG 3 cut(s) 340, 685, 1316
Bse3DI GCAATG 1 cut(s) 1467
Bse8I GATNNNNATC 1 cut(s) 1383
BseBI CCWGG 2 cut(s) 1038, 1058
BseCI ATCGAT 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 1489
BseGI GGATG 3 cut(s) 193, 558, 1117
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 6 cut(s) 70, 264, 449, 866, 1063, 1108
BseMI GCAATG 1 cut(s) 1467
BseMII CTCAG 2 cut(s) 1284, 1413
BseNI ACTGG 3 cut(s) 340, 685, 1316
BseRI GAGGAG 2 cut(s) 599, 848
BseX3I CGGCCG 1 cut(s) 597
BseXI GCAGC 4 cut(s) 370, 609, 658, 935
BseYI CCCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 1472
Bsh1236I CGCG 4 cut(s) 348, 350, 627, 632
Bsh1285I CGRYCG 1 cut(s) 600
BshFI GGCC 7 cut(s) 150, 563, 599, 802, 1036, 1101, 1494
BshVI ATCGAT 1 cut(s) 245
BsiEI CGRYCG 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 4 cut(s) 443, 460, 538, 694
BslFI GGGAC 1 cut(s) 1403
BslI CCNNNNNNNGG 6 cut(s) 70, 264, 449, 866, 1063, 1108
BsmAI GTCTC 2 cut(s) 348, 392
BsmFI GGGAC 1 cut(s) 1403
BsnI GGCC 7 cut(s) 150, 563, 599, 802, 1036, 1101, 1494
BsoBI CYCGRG 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 704
Bsp1407I TGTACA 1 cut(s) 879
Bsp143I GATC 5 cut(s) 246, 301, 576, 997, 1462
Bsp19I CCATGG 1 cut(s) 1489
BspANI GGCC 7 cut(s) 150, 563, 599, 802, 1036, 1101, 1494
BspCNI CTCAG 2 cut(s) 1285, 1414
BspDI ATCGAT 1 cut(s) 245
BspFNI CGCG 4 cut(s) 348, 350, 627, 632
BspLI GGNNCC 5 cut(s) 453, 1100, 1139, 1164, 1193
BspPI GGATC 3 cut(s) 241, 992, 1470
BspQI GCTCTTC 1 cut(s) 1365
BspT104I TTCGAA 1 cut(s) 704
BsrBI CCGCTC 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 1467
BsrGI TGTACA 1 cut(s) 879
BsrI ACTGG 3 cut(s) 340, 685, 1316
BssECI CCNNGG 1 cut(s) 1489
BssMI GATC 5 cut(s) 246, 301, 576, 997, 1462
BssT1I CCWWGG 1 cut(s) 1489
Bst2UI CCWGG 2 cut(s) 1038, 1058
Bst4CI ACNGT 2 cut(s) 775, 982
Bst6I CTCTTC 4 cut(s) 189, 207, 1280, 1365
BstAPI GCANNNNNTGC 1 cut(s) 1505
BstAUI TGTACA 1 cut(s) 879
BstBI TTCGAA 1 cut(s) 704
BstC8I GCNNGC 7 cut(s) 686, 847, 1034, 1280, 1333, 1455, 1501
BstDEI CTNAG 4 cut(s) 26, 1293, 1388, 1422
BstDSI CCRYGG 1 cut(s) 1489
BstENI CCTNNNNNAGG 1 cut(s) 1106
BstF5I GGATG 3 cut(s) 193, 558, 1117
BstFNI CGCG 4 cut(s) 348, 350, 627, 632
BstHHI GCGC 3 cut(s) 350, 627, 954
BstKTI GATC 5 cut(s) 249, 304, 579, 1000, 1465
BstMAI GTCTC 2 cut(s) 348, 392
BstMBI GATC 5 cut(s) 246, 301, 576, 997, 1462
BstMCI CGRYCG 1 cut(s) 600
BstNI CCWGG 2 cut(s) 1038, 1058
BstNSI RCATGY 1 cut(s) 1202
BstPAI GACNNNNGTC 1 cut(s) 1295
BstSCI CCNGG 3 cut(s) 537, 1036, 1056
BstSFI CTRYAG 2 cut(s) 1304, 1514
BstUI CGCG 4 cut(s) 348, 350, 627, 632
BstV1I GCAGC 4 cut(s) 370, 609, 658, 935
BstV2I GAAGAC 2 cut(s) 278, 968
BstZI CGGCCG 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 245
BsuRI GGCC 7 cut(s) 150, 563, 599, 802, 1036, 1101, 1494
BsuTUI ATCGAT 1 cut(s) 245
BtgI CCRYGG 1 cut(s) 1489
BtsCI GGATG 3 cut(s) 193, 558, 1117
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 5 cut(s) 803, 987, 1323, 1455, 1503
Cac8I GCNNGC 7 cut(s) 686, 847, 1034, 1280, 1333, 1455, 1501
CaiI CAGNNNCTG 1 cut(s) 1505
CfoI GCGC 3 cut(s) 350, 627, 954
Cfr13I GGNCC 5 cut(s) 149, 337, 452, 1099, 1191
ClaI ATCGAT 1 cut(s) 245
CseI GACGC 1 cut(s) 638
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 3 cut(s) 292, 505, 880
CviAII CATG 7 cut(s) 70, 317, 574, 890, 1199, 1360, 1490
CviQI GTAC 3 cut(s) 292, 505, 880
DdeI CTNAG 4 cut(s) 26, 1293, 1388, 1422
DpnI GATC 5 cut(s) 248, 303, 578, 999, 1464
DpnII GATC 5 cut(s) 246, 301, 576, 997, 1462
EaeI YGGCCR 1 cut(s) 597
EagI CGGCCG 1 cut(s) 597
Eam1104I CTCTTC 4 cut(s) 189, 207, 1280, 1365
EarI CTCTTC 4 cut(s) 189, 207, 1280, 1365
EclXI CGGCCG 1 cut(s) 597
Eco130I CCWWGG 1 cut(s) 1489
Eco32I GATATC 1 cut(s) 1399
Eco47I GGWCC 3 cut(s) 337, 452, 1191
Eco52I CGGCCG 1 cut(s) 597
Eco57I CTGAAG 3 cut(s) 779, 1127, 1431
Eco88I CYCGRG 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 1106
EcoO109I RGGNCCY 2 cut(s) 452, 1099
EcoRII CCWGG 2 cut(s) 1036, 1056
EcoRV GATATC 1 cut(s) 1399
EcoT14I CCWWGG 1 cut(s) 1489
ErhI CCWWGG 1 cut(s) 1489
FaeI CATG 7 cut(s) 73, 320, 577, 893, 1202, 1363, 1493
FaqI GGGAC 1 cut(s) 1403
FatI CATG 7 cut(s) 69, 316, 573, 889, 1198, 1359, 1489
FauI CCCGC 3 cut(s) 409, 442, 642
FauNDI CATATG 1 cut(s) 665
Fnu4HI GCNGC 9 cut(s) 378, 381, 384, 387, 447, 623, 647, 689, 924
FokI GGATG 3 cut(s) 200, 565, 1124
Fsp4HI GCNGC 9 cut(s) 378, 381, 384, 387, 447, 623, 647, 689, 924
FspBI CTAG 1 cut(s) 1458
GlaI GCGC 3 cut(s) 349, 626, 953
GluI GCNGC 9 cut(s) 378, 381, 384, 387, 447, 623, 647, 689, 924
GsaI CCCAGC 1 cut(s) 235
HaeIII GGCC 7 cut(s) 150, 563, 599, 802, 1036, 1101, 1494
HapII CCGG 4 cut(s) 443, 460, 538, 694
HgaI GACGC 1 cut(s) 638
HhaI GCGC 3 cut(s) 350, 627, 954
Hin1II CATG 7 cut(s) 73, 320, 577, 893, 1202, 1363, 1493
Hin6I GCGC 3 cut(s) 348, 625, 952
HinP1I GCGC 3 cut(s) 348, 625, 952
HinfI GANTC 5 cut(s) 274, 358, 425, 593, 634
HpaII CCGG 4 cut(s) 443, 460, 538, 694
HphI GGTGA 4 cut(s) 160, 353, 449, 925
Hpy166II GTNNAC 1 cut(s) 882
Hpy188I TCNGA 7 cut(s) 301, 328, 424, 526, 552, 1107, 1230
Hpy188III TCNNGA 4 cut(s) 32, 749, 852, 1084
Hpy8I GTNNAC 1 cut(s) 882
Hpy99I CGWCG 3 cut(s) 530, 533, 632
HpyAV CCTTC 3 cut(s) 1102, 1112, 1213
HpyCH4III ACNGT 2 cut(s) 775, 982
HpyCH4IV ACGT 2 cut(s) 519, 709
HpyCH4V TGCA 9 cut(s) 663, 923, 985, 1253, 1282, 1345, 1453, 1486, 1499
HpyF3I CTNAG 4 cut(s) 26, 1293, 1388, 1422
HpySE526I ACGT 2 cut(s) 519, 709
Hsp92II CATG 7 cut(s) 73, 320, 577, 893, 1202, 1363, 1493
HspAI GCGC 3 cut(s) 348, 625, 952
Kzo9I GATC 5 cut(s) 246, 301, 576, 997, 1462
LguI GCTCTTC 1 cut(s) 1365
LmnI GCTCC 4 cut(s) 205, 586, 696, 910
Lsp1109I GCAGC 4 cut(s) 370, 609, 658, 935
LweI GCATC 3 cut(s) 732, 1234, 1369
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 1 cut(s) 1458
MaeII ACGT 2 cut(s) 519, 709
MaeIII GTNAC 2 cut(s) 601, 734
MalI GATC 5 cut(s) 248, 303, 578, 999, 1464
MbiI CCGCTC 1 cut(s) 691
MboI GATC 5 cut(s) 246, 301, 576, 997, 1462
MluCI AATT 7 cut(s) 6, 332, 406, 897, 1129, 1215, 1477
MlyI GAGTC 3 cut(s) 268, 352, 434
MmeI TCCRAC 1 cut(s) 549
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 1 cut(s) 980
MspA1I CMGCKG 1 cut(s) 649
MspI CCGG 4 cut(s) 443, 460, 538, 694
MspR9I CCNGG 3 cut(s) 539, 1038, 1058
MvaI CCWGG 2 cut(s) 1038, 1058
MvnI CGCG 4 cut(s) 348, 350, 627, 632
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 1 cut(s) 1489
NdeI CATATG 1 cut(s) 665
NdeII GATC 5 cut(s) 246, 301, 576, 997, 1462
NlaIII CATG 7 cut(s) 73, 320, 577, 893, 1202, 1363, 1493
NlaIV GGNNCC 5 cut(s) 453, 1100, 1139, 1164, 1193
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 2 cut(s) 601, 734
NspI RCATGY 1 cut(s) 1202
NspV TTCGAA 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 276
PciSI GCTCTTC 1 cut(s) 1365
PcsI WCGNNNNNNNCGW 2 cut(s) 525, 602
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 2 cut(s) 593, 634
PflMI CCANNNNNTGG 2 cut(s) 866, 1063
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 9 cut(s) 379, 382, 385, 388, 448, 624, 648, 690, 925
PleI GAGTC 3 cut(s) 268, 352, 433
PpsI GAGTC 3 cut(s) 268, 352, 433
PpuMI RGGWCCY 1 cut(s) 452
PshAI GACNNNNGTC 1 cut(s) 1295
Psp1406I AACGTT 2 cut(s) 519, 709
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 1036, 1056
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 2 cut(s) 1036, 1056
PspN4I GGNNCC 5 cut(s) 453, 1100, 1139, 1164, 1193
PspPI GGNCC 5 cut(s) 149, 337, 452, 1099, 1191
PspPPI RGGWCCY 1 cut(s) 452
PspXI VCTCGAGB 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 1505
RsaI GTAC 3 cut(s) 293, 506, 881
RsaNI GTAC 3 cut(s) 292, 505, 880
RseI CAYNNNNRTG 1 cut(s) 980
SapI GCTCTTC 1 cut(s) 1365
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 9 cut(s) 378, 381, 384, 387, 447, 623, 647, 689, 924
Sau3AI GATC 5 cut(s) 246, 301, 576, 997, 1462
Sau96I GGNCC 5 cut(s) 149, 337, 452, 1099, 1191
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 3 cut(s) 268, 352, 434
ScrFI CCNGG 3 cut(s) 539, 1038, 1058
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 3 cut(s) 732, 1234, 1369
SfcI CTRYAG 2 cut(s) 1304, 1514
Sfr274I CTCGAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 704
SinI GGWCC 3 cut(s) 337, 452, 1191
SlaI CTCGAG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 980
SmlI CTYRAG 2 cut(s) 276, 749
SmoI CTYRAG 2 cut(s) 276, 749
Sse9I AATT 7 cut(s) 6, 332, 406, 897, 1129, 1215, 1477
SspMI CTAG 1 cut(s) 1458
StyD4I CCNGG 3 cut(s) 537, 1036, 1056
StyI CCWWGG 1 cut(s) 1489
TaaI ACNGT 2 cut(s) 775, 982
TaiI ACGT 2 cut(s) 522, 712
TaqI TCGA 5 cut(s) 245, 277, 410, 704, 875
TasI AATT 7 cut(s) 6, 332, 406, 897, 1129, 1215, 1477
TatI WGTACW 3 cut(s) 291, 504, 879
TauI GCSGC 5 cut(s) 380, 383, 389, 449, 691
TfiI GAWTC 2 cut(s) 593, 634
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 5 cut(s) 803, 987, 1323, 1455, 1510
TseFI GTSAC 2 cut(s) 601, 734
TseI GCWGC 4 cut(s) 383, 622, 646, 923
Tsp45I GTSAC 2 cut(s) 601, 734
TspDTI ATGAA 3 cut(s) 299, 833, 1383
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 5 cut(s) 803, 987, 1323, 1455, 1510
Van91I CCANNNNNTGG 2 cut(s) 866, 1063
VpaK11BI GGWCC 3 cut(s) 337, 452, 1191
XagI CCTNNNNNAGG 1 cut(s) 1106
XapI RAATTY 1 cut(s) 1129
XceI RCATGY 1 cut(s) 1202
XhoI CTCGAG 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 1 cut(s) 1458
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.