pycom02g06720

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015894
Physical Location & Seq
Forward (+)
4671220 .. 4672893
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06720.1

Sequence Viewer

Length: 1674 bp
ATGTCCCCAGTCCAAGTCCTTCAGCCAACATCTCCATCTCCACCAGTTCACCTCCGCACTCGCGATTCAAGTTCACACAGGCATTTCATCAAACACCTTTCAGACGCATCAAAGTCCACACCTTTCATGGAAATTACTCCGTTCCCATTTCTTAATCAAGAAGATTACACCCATTTCTACACCCTGTTCACTGACATGGACGAATTCGACATAAATGGAGGGAATCACAACCTGAAGAAGCGAAGAAAGGACGACGGAAACAACAATGGTATTGGTGGCGGCGCTGGAGCCGCTCCGGGAGCAATGAGCGACATACTGGCTTCGCTGATTCTGCTGGACGAGGAGGAGAAGCAGGAGCAGGAGAGCTACTTGGTCGATGCCCAGCAGGAAAGATTGTTGCTTGAAGCTAATCACAGGCAGAAGACTCGAGCTATGGAGGAGTATTACACTGATCTTCAGCAGCATCATTCTCAATCTGATGAATCGGAGCAGTCGAGGGCGAAACGGAGTCGGCAGTCCGCTTCCGCTGCAGCCGCTGCGGCCACTGCCGCAGCTGAGACATCCGCGGCTGGTCCGGAAGCGAGTTCGAGTGCTACGATTCCGGGTCCGCACAGGAGGCTTTGGGTGAAGGACAGGTCCAAGGTGTGGTGGGAGAATTTGGGCCACCCAGACGTTTCGGAGGAGGAGTTTCGAAAGGCGTTTCGGATGAGCAAGGGAACGTTTGACATGATATGCGCGGAGCTGGAGTCGGCGGTTTTCAAGAAGAACACAATGCTGCGCGAGGCGATTCCGGTGCGGCAGCGCGTGGCTGTGTGCATATATCGGCTTGCCACCGGGGAGCCGCTCCGGGAGGTTTCCAAACGTTTCGGGCTTGGAATCTCCACCTGCCACAAGCTGGTTCTGGAAGTTTGCCTTGCTATCAAGACTGTCTTGATGCCCAAGTTTCTCCAGTGGCCGGACGAAGGCAGGATGCAGGAAATCAAGGACGAGTTCGAGACCATTTCCGGAATTCCAAACGTTGCTGGGTCTATGTACACCACCCACATTCCAATTATTGCTCCAAAGGTGAACGTGGCTGCGTATTTCAACAAGCGGCACACGGAACGCAACCAGAAGACTTCGTATTCGATCACGGTTCAAGGGGTTGTCGATCCGAGAGGCGTTTTCACCGACGTTTGCATCGGCTGGCCGGGTTCGATGCCGGACGAACAGGTGCTGGAGAAGTCTGCGTTGTACCAGAGAGCAACCAGAGGACTATTGAAGGATGTGTGGATTGCAGGAAATGCTGGGTTGCCTCTGATGGATTGGGTGCTGGTTCCTTACACCCACCAGAACTTGACGTGGACCCAGCACGCATTCAACGAGAAAATCGGAGAGGTCGAAAGGGTGGCTAAAGATGCATTTGCAAAGTTGAAAGGGAGGTGGTCATGCTTGCAGAAGAGGACTGAGGTCAAACTGCAGGACTTGCCTGTGGTGCTTGGAGCCTGCTGTGTTTTGCACAACATCTGTGCAATGAGGAATGAGGAGCTCGATCCCGAGTTCGGGTTCGAGCTCTTCGACGATGTGGTGACACCGGTGAATAGTCTGAGGTCGGCGGGTTCGGTGCAGGCGAGGGATCACATTGCTCATAATTTGTTGCACCATGGCCTTGCTGCAGGCACTGGTTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

558

Amino Acids

62.32

Weight (kDa)

6.41

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 227 - 307 3.4e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 345 - 501 3.6e-33 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 893
AccB7I CCANNNNNTGG 2 cut(s) 645, 895
AccBSI CCGCTC 2 cut(s) 293, 844
AccII CGCG 5 cut(s) 63, 566, 737, 780, 804
AccIII TCCGGA 2 cut(s) 574, 1004
AclI AACGTT 3 cut(s) 719, 862, 1017
AclWI GGATC 3 cut(s) 1145, 1526, 1623
AcoI YGGCCR 3 cut(s) 540, 953, 1187
AcsI RAATTY 3 cut(s) 203, 655, 1008
AcuI CTGAAG 3 cut(s) 5, 254, 440
AfaI GTAC 2 cut(s) 1034, 1235
AfiI CCNNNNNNNGG 6 cut(s) 645, 895, 955, 962, 1541, 1542
AgeI ACCGGT 1 cut(s) 1573
AgsI TTSAA 8 cut(s) 69, 404, 760, 1087, 1139, 1261, 1360, 1414
AjiI CACGTC 1 cut(s) 1341
AluBI AGCT 8 cut(s) 366, 407, 431, 554, 742, 895, 1528, 1552
AluI AGCT 8 cut(s) 366, 407, 431, 554, 742, 895, 1528, 1552
Alw21I GWGCWC 2 cut(s) 1530, 1554
Alw26I GTCTC 2 cut(s) 551, 989
AlwI GGATC 3 cut(s) 1145, 1526, 1623
AlwNI CAGNNNCTG 3 cut(s) 536, 1216, 1661
Ama87I CYCGRG 2 cut(s) 426, 1535
Aor13HI TCCGGA 2 cut(s) 574, 1004
AoxI GGCC 5 cut(s) 540, 661, 953, 1187, 1645
ApeKI GCWGC 9 cut(s) 460, 527, 530, 536, 551, 775, 799, 1076, 1652
ApoI RAATTY 3 cut(s) 203, 655, 1008
ArsI GACNNNNNNTTYG 2 cut(s) 1108, 1140
AsiGI ACCGGT 1 cut(s) 1573
AspLEI GCGC 4 cut(s) 284, 737, 780, 804
AspS9I GGNCC 5 cut(s) 572, 605, 636, 661, 1344
AsuC2I CCSGG 5 cut(s) 297, 603, 835, 848, 1191
AsuHPI GGTGA 6 cut(s) 41, 637, 1078, 1159, 1579, 1588
AsuII TTCGAA 1 cut(s) 691
AvaI CYCGRG 2 cut(s) 426, 1535
AvaII GGWCC 4 cut(s) 572, 605, 636, 1344
BanII GRGCYC 2 cut(s) 1530, 1554
BbsI GAAGAC 2 cut(s) 428, 1121
Bbv12I GWGCWC 2 cut(s) 1530, 1554
BbvI GCAGC 9 cut(s) 472, 514, 523, 542, 563, 762, 811, 1063, 1639
BccI CCATC 2 cut(s) 43, 1294
BcgI CGANNNNNNTGC 4 cut(s) 407, 441, 775, 809
BcnI CCSGG 5 cut(s) 297, 603, 835, 848, 1191
BcoDI GTCTC 2 cut(s) 551, 989
BfmI CTRYAG 4 cut(s) 528, 1457, 1653, 1670
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 893
BglI GCCNNNNNGGC 1 cut(s) 539
Bme1390I CCNGG 5 cut(s) 297, 603, 835, 848, 1191
Bme18I GGWCC 4 cut(s) 572, 605, 636, 1344
BmeT110I CYCGRG 2 cut(s) 426, 1535
BmgBI CACGTC 1 cut(s) 1341
BmgT120I GGNCC 5 cut(s) 572, 605, 636, 661, 1344
BmiI GGNNCC 6 cut(s) 289, 606, 840, 1317, 1346, 1483
BmrFI CCNGG 5 cut(s) 297, 603, 835, 848, 1191
BmsI GCATC 8 cut(s) 116, 367, 472, 924, 960, 1188, 1188, 1387
BoxI GACNNNNGTC 1 cut(s) 1448
BpiI GAAGAC 2 cut(s) 428, 1121
BpmI CTGGAG 4 cut(s) 306, 764, 932, 1238
Bpu14I TTCGAA 1 cut(s) 691
BpuMI CCSGG 5 cut(s) 297, 603, 835, 848, 1191
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 4 cut(s) 564, 639, 834, 1642
BsaWI WCCGGW 4 cut(s) 574, 790, 1004, 1573
BsaXI ACNNNNNCTCC 4 cut(s) 671, 701, 731, 761
Bsc4I CCNNNNNNNGG 6 cut(s) 645, 895, 955, 962, 1541, 1542
Bse118I RCCGGY 1 cut(s) 1573
Bse1I ACTGG 5 cut(s) 8, 44, 321, 949, 1666
Bse3DI GCAATG 3 cut(s) 309, 1518, 1620
BseAI TCCGGA 2 cut(s) 574, 1004
BseDI CCNNGG 4 cut(s) 564, 639, 834, 1642
BseGI GGATG 4 cut(s) 560, 711, 975, 1270
BseLI CCNNNNNNNGG 6 cut(s) 645, 895, 955, 962, 1541, 1542
BseMI GCAATG 3 cut(s) 309, 1518, 1620
BseMII CTCAG 3 cut(s) 546, 1437, 1577
BseNI ACTGG 5 cut(s) 8, 44, 321, 949, 1666
BseRI GAGGAG 6 cut(s) 356, 359, 452, 695, 698, 1538
BseXI GCAGC 9 cut(s) 472, 514, 523, 542, 563, 762, 811, 1063, 1639
BseYI CCCAGC 4 cut(s) 381, 1022, 1286, 1347
BsgI GTGCAG 1 cut(s) 1625
Bsh1236I CGCG 5 cut(s) 63, 566, 737, 780, 804
BshFI GGCC 5 cut(s) 542, 663, 955, 1189, 1647
BshTI ACCGGT 1 cut(s) 1573
BsiHKAI GWGCWC 2 cut(s) 1530, 1554
BsiHKCI CYCGRG 2 cut(s) 426, 1535
BslI CCNNNNNNNGG 6 cut(s) 645, 895, 955, 962, 1541, 1542
BsmAI GTCTC 2 cut(s) 551, 989
BsmI GAATGC 1 cut(s) 1355
BsnI GGCC 5 cut(s) 542, 663, 955, 1189, 1647
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 2 cut(s) 426, 1535
Bsp119I TTCGAA 1 cut(s) 691
Bsp1286I GDGCHC 2 cut(s) 1530, 1554
Bsp13I TCCGGA 2 cut(s) 574, 1004
Bsp1407I TGTACA 1 cut(s) 1032
Bsp143I GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
Bsp19I CCATGG 1 cut(s) 1642
Bsp68I TCGCGA 1 cut(s) 63
BspANI GGCC 5 cut(s) 542, 663, 955, 1189, 1647
BspCNI CTCAG 3 cut(s) 547, 1438, 1578
BspEI TCCGGA 2 cut(s) 574, 1004
BspFNI CGCG 5 cut(s) 63, 566, 737, 780, 804
BspLI GGNNCC 6 cut(s) 289, 606, 840, 1317, 1346, 1483
BspMAI CTGCAG 3 cut(s) 532, 1461, 1657
BspMI ACCTGC 1 cut(s) 893
BspPI GGATC 3 cut(s) 1145, 1526, 1623
BspQI GCTCTTC 1 cut(s) 1559
BspT104I TTCGAA 1 cut(s) 691
BspTNI GGTCTC 1 cut(s) 989
BsrBI CCGCTC 2 cut(s) 293, 844
BsrDI GCAATG 3 cut(s) 309, 1518, 1620
BsrFI RCCGGY 1 cut(s) 1573
BsrGI TGTACA 1 cut(s) 1032
BsrI ACTGG 5 cut(s) 8, 44, 321, 949, 1666
BssAI RCCGGY 1 cut(s) 1573
BssECI CCNNGG 4 cut(s) 564, 639, 834, 1642
BssMI GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
BssT1I CCWWGG 2 cut(s) 639, 1642
Bst4CI ACNGT 2 cut(s) 928, 1135
Bst6I CTCTTC 2 cut(s) 1433, 1559
BstAPI GCANNNNNTGC 3 cut(s) 536, 1283, 1465
BstAUI TGTACA 1 cut(s) 1032
BstBI TTCGAA 1 cut(s) 691
BstC8I GCNNGC 7 cut(s) 828, 1187, 1353, 1433, 1486, 1608, 1657
BstDEI CTNAG 3 cut(s) 555, 1446, 1586
BstDSI CCRYGG 2 cut(s) 564, 1642
BstF5I GGATG 4 cut(s) 560, 711, 975, 1270
BstFNI CGCG 5 cut(s) 63, 566, 737, 780, 804
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 4 cut(s) 284, 737, 780, 804
BstKTI GATC 5 cut(s) 454, 1131, 1153, 1534, 1618
BstMAI GTCTC 2 cut(s) 551, 989
BstMBI GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
BstPAI GACNNNNGTC 1 cut(s) 1448
BstSCI CCNGG 5 cut(s) 295, 601, 833, 846, 1189
BstSFI CTRYAG 4 cut(s) 528, 1457, 1653, 1670
BstUI CGCG 5 cut(s) 63, 566, 737, 780, 804
BstV1I GCAGC 9 cut(s) 472, 514, 523, 542, 563, 762, 811, 1063, 1639
BstV2I GAAGAC 2 cut(s) 428, 1121
BsuRI GGCC 5 cut(s) 542, 663, 955, 1189, 1647
BtgI CCRYGG 2 cut(s) 564, 1642
BtrI CACGTC 1 cut(s) 1341
BtsCI GGATG 4 cut(s) 560, 711, 975, 1270
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 5 cut(s) 189, 447, 543, 956, 1659
BtuMI TCGCGA 1 cut(s) 63
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 7 cut(s) 828, 1187, 1353, 1433, 1486, 1608, 1657
CaiI CAGNNNCTG 3 cut(s) 536, 1216, 1661
CfoI GCGC 4 cut(s) 284, 737, 780, 804
Cfr10I RCCGGY 1 cut(s) 1573
Cfr13I GGNCC 5 cut(s) 572, 605, 636, 661, 1344
Cfr42I CCGCGG 1 cut(s) 567
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 1033, 1234
CspAI ACCGGT 1 cut(s) 1573
CviAII CATG 5 cut(s) 127, 196, 727, 1428, 1643
CviQI GTAC 2 cut(s) 1033, 1234
DdeI CTNAG 3 cut(s) 555, 1446, 1586
DpnI GATC 5 cut(s) 453, 1130, 1152, 1533, 1617
DpnII GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
EaeI YGGCCR 3 cut(s) 540, 953, 1187
Eam1104I CTCTTC 2 cut(s) 1433, 1559
EarI CTCTTC 2 cut(s) 1433, 1559
Ecl136II GAGCTC 2 cut(s) 1528, 1552
Eco130I CCWWGG 2 cut(s) 639, 1642
Eco24I GRGCYC 2 cut(s) 1530, 1554
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 4 cut(s) 572, 605, 636, 1344
Eco53kI GAGCTC 2 cut(s) 1528, 1552
Eco57I CTGAAG 3 cut(s) 5, 254, 440
Eco88I CYCGRG 2 cut(s) 426, 1535
EcoICRI GAGCTC 2 cut(s) 1528, 1552
EcoRI GAATTC 2 cut(s) 203, 1008
EcoT14I CCWWGG 2 cut(s) 639, 1642
EcoT22I ATGCAT 1 cut(s) 1402
EcoT38I GRGCYC 2 cut(s) 1530, 1554
ErhI CCWWGG 2 cut(s) 639, 1642
FaeI CATG 5 cut(s) 130, 199, 730, 1431, 1646
FalI AAGNNNNNCTT 4 cut(s) 897, 929, 914, 946
FatI CATG 5 cut(s) 126, 195, 726, 1427, 1642
FauI CCCGC 1 cut(s) 1588
FokI GGATG 4 cut(s) 547, 718, 982, 1277
FriOI GRGCYC 2 cut(s) 1530, 1554
GlaI GCGC 4 cut(s) 283, 736, 779, 803
GsaI CCCAGC 4 cut(s) 385, 1026, 1290, 1351
GsuI CTGGAG 4 cut(s) 306, 764, 932, 1238
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 5 cut(s) 542, 663, 955, 1189, 1647
HgaI GACGC 1 cut(s) 113
HhaI GCGC 4 cut(s) 284, 737, 780, 804
Hin1II CATG 5 cut(s) 130, 199, 730, 1431, 1646
Hin6I GCGC 4 cut(s) 282, 735, 778, 802
HinP1I GCGC 4 cut(s) 282, 735, 778, 802
HphI GGTGA 6 cut(s) 41, 637, 1078, 1159, 1579, 1588
Hpy166II GTNNAC 7 cut(s) 49, 74, 117, 189, 1035, 1069, 1344
Hpy188I TCNGA 9 cut(s) 103, 478, 487, 679, 705, 1155, 1299, 1373, 1587
Hpy8I GTNNAC 7 cut(s) 49, 74, 117, 189, 1035, 1069, 1344
Hpy99I CGWCG 3 cut(s) 257, 1175, 1562
HpyAV CCTTC 4 cut(s) 29, 622, 956, 1255
HpyCH4III ACNGT 2 cut(s) 928, 1135
HpyCH4IV ACGT 7 cut(s) 672, 719, 862, 1017, 1071, 1173, 1340
HpyF3I CTNAG 3 cut(s) 555, 1446, 1586
HpySE526I ACGT 7 cut(s) 672, 719, 862, 1017, 1071, 1173, 1340
Hsp92II CATG 5 cut(s) 130, 199, 730, 1431, 1646
HspAI GCGC 4 cut(s) 282, 735, 778, 802
Kpn2I TCCGGA 2 cut(s) 574, 1004
KspI CCGCGG 1 cut(s) 567
Kzo9I GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
LguI GCTCTTC 1 cut(s) 1559
Lsp1109I GCAGC 9 cut(s) 472, 514, 523, 542, 563, 762, 811, 1063, 1639
LweI GCATC 8 cut(s) 116, 367, 472, 924, 960, 1188, 1188, 1387
MaeII ACGT 7 cut(s) 672, 719, 862, 1017, 1071, 1173, 1340
MaeIII GTNAC 1 cut(s) 1567
MalI GATC 5 cut(s) 453, 1130, 1152, 1533, 1617
MbiI CCGCTC 2 cut(s) 293, 844
MboI GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
MboII GAAGA 9 cut(s) 173, 247, 255, 433, 446, 775, 1126, 1450, 1546
MhlI GDGCHC 2 cut(s) 1530, 1554
MluCI AATT 6 cut(s) 132, 203, 655, 1008, 1050, 1630
MlyI GAGTC 3 cut(s) 418, 517, 755
Mph1103I ATGCAT 1 cut(s) 1402
MroI TCCGGA 2 cut(s) 574, 1004
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 4 cut(s) 527, 536, 554, 566
MspR9I CCNGG 5 cut(s) 297, 603, 835, 848, 1191
Mva1269I GAATGC 1 cut(s) 1355
MvnI CGCG 5 cut(s) 63, 566, 737, 780, 804
NciI CCSGG 5 cut(s) 297, 603, 835, 848, 1191
NcoI CCATGG 1 cut(s) 1642
NdeII GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
NlaIII CATG 5 cut(s) 130, 199, 730, 1431, 1646
NlaIV GGNNCC 6 cut(s) 289, 606, 840, 1317, 1346, 1483
NmuCI GTSAC 1 cut(s) 1567
NruI TCGCGA 1 cut(s) 63
NsiI ATGCAT 1 cut(s) 1402
NspV TTCGAA 1 cut(s) 691
PaeR7I CTCGAG 1 cut(s) 426
PaqCI CACCTGC 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 1559
PcsI WCGNNNNNNNCGW 6 cut(s) 491, 593, 1359, 1377, 1554, 1607
PctI GAATGC 1 cut(s) 1355
PfeI GAWTC 7 cut(s) 65, 223, 328, 482, 598, 787, 876
PflMI CCANNNNNTGG 2 cut(s) 645, 895
PfoI TCCNGGA 2 cut(s) 295, 846
PinAI ACCGGT 1 cut(s) 1573
PleI GAGTC 3 cut(s) 418, 516, 754
PpsI GAGTC 3 cut(s) 418, 516, 754
PshAI GACNNNNGTC 1 cut(s) 1448
Psp124BI GAGCTC 2 cut(s) 1530, 1554
Psp1406I AACGTT 3 cut(s) 719, 862, 1017
PspFI CCCAGC 4 cut(s) 381, 1022, 1286, 1347
PspN4I GGNNCC 6 cut(s) 289, 606, 840, 1317, 1346, 1483
PspPI GGNCC 5 cut(s) 572, 605, 636, 661, 1344
PspXI VCTCGAGB 1 cut(s) 426
PstI CTGCAG 3 cut(s) 532, 1461, 1657
PstNI CAGNNNCTG 3 cut(s) 536, 1216, 1661
PvuII CAGCTG 1 cut(s) 554
RruI TCGCGA 1 cut(s) 63
RsaI GTAC 2 cut(s) 1034, 1235
RsaNI GTAC 2 cut(s) 1033, 1234
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 2 cut(s) 1530, 1554
SacII CCGCGG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 1559
SaqAI TTAA 1 cut(s) 153
Sau3AI GATC 5 cut(s) 451, 1128, 1150, 1531, 1615
Sau96I GGNCC 5 cut(s) 572, 605, 636, 661, 1344
SchI GAGTC 3 cut(s) 418, 517, 755
ScrFI CCNGG 5 cut(s) 297, 603, 835, 848, 1191
SduI GDGCHC 2 cut(s) 1530, 1554
SfaNI GCATC 8 cut(s) 116, 367, 472, 924, 960, 1188, 1188, 1387
SfcI CTRYAG 4 cut(s) 528, 1457, 1653, 1670
Sfr274I CTCGAG 1 cut(s) 426
Sfr303I CCGCGG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 691
SgrAI CRCCGGYG 1 cut(s) 1573
SgrBI CCGCGG 1 cut(s) 567
SinI GGWCC 4 cut(s) 572, 605, 636, 1344
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 6 cut(s) 132, 203, 655, 1008, 1050, 1630
SstI GAGCTC 2 cut(s) 1530, 1554
StyD4I CCNGG 5 cut(s) 295, 601, 833, 846, 1189
StyI CCWWGG 2 cut(s) 639, 1642
TaaI ACNGT 2 cut(s) 928, 1135
TaiI ACGT 7 cut(s) 675, 722, 865, 1020, 1074, 1176, 1343
TasI AATT 6 cut(s) 132, 203, 655, 1008, 1050, 1630
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 9 cut(s) 282, 293, 536, 542, 551, 569, 799, 844, 1096
TfiI GAWTC 7 cut(s) 65, 223, 328, 482, 598, 787, 876
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 5 cut(s) 196, 454, 550, 956, 1666
TseFI GTSAC 1 cut(s) 1567
TseI GCWGC 9 cut(s) 460, 527, 530, 536, 551, 775, 799, 1076, 1652
Tsp45I GTSAC 1 cut(s) 1567
TspDTI ATGAA 3 cut(s) 76, 115, 495
TspGWI ACGGA 4 cut(s) 129, 270, 520, 1115
TspRI CASTG 5 cut(s) 196, 454, 550, 956, 1666
Van91I CCANNNNNTGG 2 cut(s) 645, 895
VpaK11BI GGWCC 4 cut(s) 572, 605, 636, 1344
XapI RAATTY 3 cut(s) 203, 655, 1008
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.