MD15G1211600.v1.1

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015894
Physical Location & Seq
Forward (+)
16999988 .. 17001403
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1211600.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGAGCGACATACTTGCCTCGCTGATTCTGCTGGACGAGGAGGAGAAGCTGGAGCAGGAGAGCTACTTGGTCGAAGCCCAGCAGGAAAGATCGATGCTTGAAGATAATCACAGGCAGAAGACTCGAGCTATGGAGGAGTACTACTCTGATATTCAGCAGCACCATTCTCAATCCGACGAATCGGACCTGTCTAGGGCGAAACGGAGTCGACAGTCCGCTTCCGCTGCGGCCGCTGCTGCAGCTGAGGCCTCCGCGGCCGGTCCGGACCCGAGTTCGAGTGCTATGATCCCGGGTCCGCACCGGAGGCTTTGGGTGAAGGACAGGCCCAAGGTGTGGTGGGAGAATTTGGGCCGCCCAGACGTTTCGGAGGAGGAGTTTCGAAAAGCTTTTCGGATGAGCAAGGGGACGTTCGACATGATCTGCGCGGAGCTGGAGTCGGCGGTTTTTAAGAAGAACACAATGCTGCGCGAGGCAATTCCGGTGCGCCAACGCGTGGCGGTGTGCATATATCGGCTTGCCACCGGGGAGCCGCTCCGGGAGGTTTCGAGACGTTTCGGGCTGGGAATCTCCACCTGCCACAAGCTGGTTCTGGAAGTTTGCGTTGCCATCAAGACTGTCTTGATGCACAAGTTTCTCCAGTGGCCGGACGAGGGCAGGATGCAGGAGATCAAGGAGGAGTTCGAGGCCACTTCCGGAATTCCAAATGTTGCTGGGTCTATGTACACCACCCACATATCAATTATTGCTCCAAAGGTGAATGTGGCTGCGTATTTCAACAAGCGGCACACGGAACGCAACCAGAAAACTTCGTATTCGGTCACGGTTCAAGGGATTGTCGATCCGAGAGGCGTTTTCACCGACGTTTGCATCGGCTGGCCGGGTTCGATGCCGGACGATCAGGTGCTGGAGAAGTCTGCATTGTTCCAGAGAGCAAACAGAGGACTCTTGAAGGATGTGTGGGTTGTGGGAAATGCTGGGTTTCCACTGATGGATTGGGTGCTGGTTCCTTACACCCACCAGAACTTGACGTGGACCCAGCACGCATTCAATGAGAAAATCGGAGAAGTGGAAGGGGTGGCTAAAGATGCATTTGCAAAGCTCAAAGGGAGGTGGTCTTGCTTGCAGAAGAGGACTGAGATCAAGCTGCAGGACTTGCCTGTTGTGCTTGGGGCTTGCTGTGTTTTGCACAATATTTGTGCAATGAGGAATGATGAGCTCGATCCCGAGTTCGAGTTCGAGCTCTTCGACGATTTGGCAACGCCGGAAAATAGTCTGAGGTCGGCGGGATCGGTGCAGGCGAGGGATCATATTGCTCATAATTTGTTGCACCATGGCCTTGCTGCAGGCACCGGTTCTGGTTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

456

Amino Acids

51.14

Weight (kDa)

6.05

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 123 - 203 2.8e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 241 - 397 5.3e-35 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 1346
AccB7I CCANNNNNTGG 3 cut(s) 333, 493, 583
AccBSI CCGCTC 1 cut(s) 532
AccI GTMKAC 1 cut(s) 208
AccII CGCG 4 cut(s) 254, 425, 468, 492
AccIII TCCGGA 2 cut(s) 262, 692
AclWI GGATC 5 cut(s) 280, 833, 1214, 1294, 1311
AcoI YGGCCR 4 cut(s) 228, 255, 641, 875
AcsI RAATTY 2 cut(s) 343, 696
AfaI GTAC 2 cut(s) 140, 722
AfiI CCNNNNNNNGG 6 cut(s) 193, 333, 493, 583, 643, 650
AflIII ACRYGT 1 cut(s) 490
AgeI ACCGGT 1 cut(s) 1349
AgsI TTSAA 5 cut(s) 101, 775, 827, 949, 1048
AjiI CACGTC 1 cut(s) 1029
Alw21I GWGCWC 2 cut(s) 1218, 1242
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 5 cut(s) 280, 833, 1214, 1294, 1311
AlwNI CAGNNNCTG 1 cut(s) 904
Ama87I CYCGRG 4 cut(s) 123, 268, 289, 1223
Aor13HI TCCGGA 2 cut(s) 262, 692
AoxI GGCC 9 cut(s) 228, 246, 255, 323, 349, 641, 684, 875, 1333
ApeKI GCWGC 9 cut(s) 157, 224, 233, 236, 239, 463, 764, 1144, 1340
ApoI RAATTY 2 cut(s) 343, 696
AsiGI ACCGGT 1 cut(s) 1349
AspLEI GCGC 3 cut(s) 425, 468, 486
AspS9I GGNCC 7 cut(s) 184, 260, 265, 293, 324, 349, 1032
AsuC2I CCSGG 5 cut(s) 290, 291, 523, 536, 879
AsuHPI GGTGA 3 cut(s) 325, 766, 847
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 4 cut(s) 123, 268, 289, 1223
AvaII GGWCC 5 cut(s) 184, 260, 265, 293, 1032
BanI GGYRCC 1 cut(s) 1346
BanII GRGCYC 2 cut(s) 1218, 1242
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 1218, 1242
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 9 cut(s) 169, 211, 220, 223, 251, 450, 751, 1131, 1327
BccI CCATC 2 cut(s) 614, 982
BcgI CGANNNNNNTGC 3 cut(s) 30, 104, 138
BcnI CCSGG 5 cut(s) 290, 291, 523, 536, 879
BcoDI GTCTC 1 cut(s) 541
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 4 cut(s) 237, 1145, 1341, 1364
BfuAI ACCTGC 1 cut(s) 581
BglI GCCNNNNNGGC 1 cut(s) 254
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 5 cut(s) 290, 291, 523, 536, 879
Bme18I GGWCC 5 cut(s) 184, 260, 265, 293, 1032
BmeT110I CYCGRG 4 cut(s) 123, 268, 289, 1223
BmgBI CACGTC 1 cut(s) 1029
BmgT120I GGNCC 7 cut(s) 184, 260, 265, 293, 324, 349, 1032
BmiI GGNNCC 6 cut(s) 267, 294, 528, 1005, 1034, 1348
BmrFI CCNGG 5 cut(s) 290, 291, 523, 536, 879
BmsI GCATC 6 cut(s) 84, 612, 648, 876, 876, 1075
BpiI GAAGAC 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 4 cut(s) 71, 452, 620, 926
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 379
BpuMI CCSGG 5 cut(s) 290, 291, 523, 536, 879
Bsa29I ATCGAT 1 cut(s) 92
BsaJI CCNNGG 5 cut(s) 252, 289, 327, 522, 1330
BsaWI WCCGGW 5 cut(s) 262, 300, 478, 692, 1349
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 419, 449
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 333, 493, 583, 643, 650
Bse118I RCCGGY 2 cut(s) 257, 1349
Bse1I ACTGG 1 cut(s) 637
Bse3DI GCAATG 1 cut(s) 1206
BseAI TCCGGA 2 cut(s) 262, 692
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 5 cut(s) 252, 289, 327, 522, 1330
BseGI GGATG 3 cut(s) 399, 663, 958
BseLI CCNNNNNNNGG 6 cut(s) 193, 333, 493, 583, 643, 650
BseMI GCAATG 1 cut(s) 1206
BseMII CTCAG 3 cut(s) 234, 1125, 1265
BseNI ACTGG 1 cut(s) 637
BseRI GAGGAG 6 cut(s) 53, 56, 149, 383, 386, 689
BseX3I CGGCCG 2 cut(s) 228, 255
BseXI GCAGC 9 cut(s) 169, 211, 220, 223, 251, 450, 751, 1131, 1327
BseYI CCCAGC 5 cut(s) 78, 559, 710, 974, 1035
BsgI GTGCAG 1 cut(s) 1313
Bsh1236I CGCG 4 cut(s) 254, 425, 468, 492
Bsh1285I CGRYCG 2 cut(s) 231, 258
BshFI GGCC 9 cut(s) 230, 248, 257, 325, 351, 643, 686, 877, 1335
BshNI GGYRCC 1 cut(s) 1346
BshTI ACCGGT 1 cut(s) 1349
BshVI ATCGAT 1 cut(s) 92
BsiEI CGRYCG 2 cut(s) 231, 258
BsiHKAI GWGCWC 2 cut(s) 1218, 1242
BsiHKCI CYCGRG 4 cut(s) 123, 268, 289, 1223
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 6 cut(s) 193, 333, 493, 583, 643, 650
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 418
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 9 cut(s) 230, 248, 257, 325, 351, 643, 686, 877, 1335
BsoBI CYCGRG 4 cut(s) 123, 268, 289, 1223
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 1218, 1242
Bsp13I TCCGGA 2 cut(s) 262, 692
Bsp1407I TGTACA 1 cut(s) 720
Bsp19I CCATGG 1 cut(s) 1330
BspANI GGCC 9 cut(s) 230, 248, 257, 325, 351, 643, 686, 877, 1335
BspCNI CTCAG 3 cut(s) 235, 1126, 1266
BspDI ATCGAT 1 cut(s) 92
BspEI TCCGGA 2 cut(s) 262, 692
BspFNI CGCG 4 cut(s) 254, 425, 468, 492
BspLI GGNNCC 6 cut(s) 267, 294, 528, 1005, 1034, 1348
BspMAI CTGCAG 3 cut(s) 241, 1149, 1345
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 5 cut(s) 280, 833, 1214, 1294, 1311
BspQI GCTCTTC 1 cut(s) 1247
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 1346
BsrBI CCGCTC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 1206
BsrFI RCCGGY 2 cut(s) 257, 1349
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 1 cut(s) 637
BssAI RCCGGY 2 cut(s) 257, 1349
BssECI CCNNGG 5 cut(s) 252, 289, 327, 522, 1330
BssT1I CCWWGG 2 cut(s) 327, 1330
Bst4CI ACNGT 3 cut(s) 213, 616, 823
Bst6I CTCTTC 2 cut(s) 1121, 1247
BstAPI GCANNNNNTGC 1 cut(s) 1153
BstAUI TGTACA 1 cut(s) 720
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 7 cut(s) 516, 875, 1041, 1121, 1174, 1296, 1345
BstDEI CTNAG 3 cut(s) 243, 1134, 1274
BstDSI CCRYGG 2 cut(s) 252, 1330
BstF5I GGATG 3 cut(s) 399, 663, 958
BstFNI CGCG 4 cut(s) 254, 425, 468, 492
BstHHI GCGC 3 cut(s) 425, 468, 486
BstMAI GTCTC 1 cut(s) 541
BstMCI CGRYCG 2 cut(s) 231, 258
BstSCI CCNGG 5 cut(s) 288, 289, 521, 534, 877
BstSFI CTRYAG 4 cut(s) 237, 1145, 1341, 1364
BstUI CGCG 4 cut(s) 254, 425, 468, 492
BstV1I GCAGC 9 cut(s) 169, 211, 220, 223, 251, 450, 751, 1131, 1327
BstV2I GAAGAC 1 cut(s) 125
BstZI CGGCCG 2 cut(s) 228, 255
Bsu15I ATCGAT 1 cut(s) 92
BsuRI GGCC 9 cut(s) 230, 248, 257, 325, 351, 643, 686, 877, 1335
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 2 cut(s) 252, 1330
BtrI CACGTC 1 cut(s) 1029
BtsCI GGATG 3 cut(s) 399, 663, 958
BtsIMutI CAGTG 2 cut(s) 644, 983
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 7 cut(s) 516, 875, 1041, 1121, 1174, 1296, 1345
CaiI CAGNNNCTG 1 cut(s) 904
CciNI GCGGCCGC 1 cut(s) 228
CfoI GCGC 3 cut(s) 425, 468, 486
Cfr10I RCCGGY 2 cut(s) 257, 1349
Cfr13I GGNCC 7 cut(s) 184, 260, 265, 293, 324, 349, 1032
Cfr42I CCGCGG 1 cut(s) 255
Cfr9I CCCGGG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 92
CpoI CGGWCCG 1 cut(s) 260
Csp6I GTAC 2 cut(s) 139, 721
CspAI ACCGGT 1 cut(s) 1349
CspI CGGWCCG 1 cut(s) 260
CviAII CATG 2 cut(s) 415, 1331
CviQI GTAC 2 cut(s) 139, 721
DdeI CTNAG 3 cut(s) 243, 1134, 1274
EaeI YGGCCR 4 cut(s) 228, 255, 641, 875
EagI CGGCCG 2 cut(s) 228, 255
Eam1104I CTCTTC 2 cut(s) 1121, 1247
EarI CTCTTC 2 cut(s) 1121, 1247
Ecl136II GAGCTC 2 cut(s) 1216, 1240
EclXI CGGCCG 2 cut(s) 228, 255
Eco130I CCWWGG 2 cut(s) 327, 1330
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 2 cut(s) 1218, 1242
Eco47I GGWCC 5 cut(s) 184, 260, 265, 293, 1032
Eco52I CGGCCG 2 cut(s) 228, 255
Eco53kI GAGCTC 2 cut(s) 1216, 1240
Eco88I CYCGRG 4 cut(s) 123, 268, 289, 1223
EcoICRI GAGCTC 2 cut(s) 1216, 1240
EcoRI GAATTC 1 cut(s) 696
EcoT14I CCWWGG 2 cut(s) 327, 1330
EcoT22I ATGCAT 1 cut(s) 1090
EcoT38I GRGCYC 2 cut(s) 1218, 1242
ErhI CCWWGG 2 cut(s) 327, 1330
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 2 cut(s) 418, 1334
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 1 cut(s) 418
FatI CATG 2 cut(s) 414, 1330
FauI CCCGC 1 cut(s) 1276
FblI GTMKAC 1 cut(s) 208
FokI GGATG 3 cut(s) 406, 670, 965
FriOI GRGCYC 2 cut(s) 1218, 1242
FspBI CTAG 1 cut(s) 192
GlaI GCGC 3 cut(s) 424, 467, 485
GsaI CCCAGC 5 cut(s) 82, 563, 714, 978, 1039
GsuI CTGGAG 4 cut(s) 71, 452, 620, 926
HaeIII GGCC 9 cut(s) 230, 248, 257, 325, 351, 643, 686, 877, 1335
HhaI GCGC 3 cut(s) 425, 468, 486
Hin1II CATG 2 cut(s) 418, 1334
Hin6I GCGC 3 cut(s) 423, 466, 484
HinP1I GCGC 3 cut(s) 423, 466, 484
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 7 cut(s) 25, 121, 179, 205, 434, 564, 942
HphI GGTGA 3 cut(s) 325, 766, 847
Hpy166II GTNNAC 3 cut(s) 209, 723, 1032
Hpy188I TCNGA 8 cut(s) 148, 175, 184, 367, 393, 843, 1061, 1275
Hpy188III TCNNGA 9 cut(s) 263, 546, 590, 610, 619, 693, 925, 946, 1223
Hpy8I GTNNAC 3 cut(s) 209, 723, 1032
Hpy99I CGWCG 3 cut(s) 179, 863, 1250
HpyAV CCTTC 3 cut(s) 310, 943, 1064
HpyCH4III ACNGT 3 cut(s) 213, 616, 823
HpyCH4IV ACGT 5 cut(s) 360, 407, 550, 861, 1028
HpyF3I CTNAG 3 cut(s) 243, 1134, 1274
HpySE526I ACGT 5 cut(s) 360, 407, 550, 861, 1028
Hsp92II CATG 2 cut(s) 418, 1334
HspAI GCGC 3 cut(s) 423, 466, 484
Kpn2I TCCGGA 2 cut(s) 262, 692
KspI CCGCGG 1 cut(s) 255
LguI GCTCTTC 1 cut(s) 1247
LmnI GCTCC 5 cut(s) 52, 427, 526, 537, 751
Lsp1109I GCAGC 9 cut(s) 169, 211, 220, 223, 251, 450, 751, 1131, 1327
LweI GCATC 6 cut(s) 84, 612, 648, 876, 876, 1075
MaeI CTAG 1 cut(s) 192
MaeII ACGT 5 cut(s) 360, 407, 550, 861, 1028
MaeIII GTNAC 1 cut(s) 817
MbiI CCGCTC 1 cut(s) 532
MboII GAAGA 5 cut(s) 113, 130, 463, 1138, 1234
MhlI GDGCHC 2 cut(s) 1218, 1242
MluCI AATT 5 cut(s) 343, 474, 696, 738, 1318
MluI ACGCGT 1 cut(s) 490
MlyI GAGTC 4 cut(s) 115, 214, 443, 936
MmeI TCCRAC 1 cut(s) 198
Mph1103I ATGCAT 1 cut(s) 1090
MroI TCCGGA 2 cut(s) 262, 692
MseI TTAA 1 cut(s) 447
MspA1I CMGCKG 4 cut(s) 224, 233, 242, 254
MspR9I CCNGG 5 cut(s) 290, 291, 523, 536, 879
Mva1269I GAATGC 1 cut(s) 1043
MvnI CGCG 4 cut(s) 254, 425, 468, 492
NciI CCSGG 5 cut(s) 290, 291, 523, 536, 879
NcoI CCATGG 1 cut(s) 1330
NlaIII CATG 2 cut(s) 418, 1334
NlaIV GGNNCC 6 cut(s) 267, 294, 528, 1005, 1034, 1348
NmuCI GTSAC 1 cut(s) 817
NotI GCGGCCGC 1 cut(s) 228
NsiI ATGCAT 1 cut(s) 1090
NspV TTCGAA 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 123
PaqCI CACCTGC 1 cut(s) 581
PceI AGGCCT 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 1247
PcsI WCGNNNNNNNCGW 2 cut(s) 1242, 1295
PctI GAATGC 1 cut(s) 1043
PfeI GAWTC 3 cut(s) 25, 179, 564
PflMI CCANNNNNTGG 3 cut(s) 333, 493, 583
PfoI TCCNGGA 1 cut(s) 534
PinAI ACCGGT 1 cut(s) 1349
PleI GAGTC 4 cut(s) 115, 213, 442, 936
PpsI GAGTC 4 cut(s) 115, 213, 442, 936
Psp124BI GAGCTC 2 cut(s) 1218, 1242
PspFI CCCAGC 5 cut(s) 78, 559, 710, 974, 1035
PspN4I GGNNCC 6 cut(s) 267, 294, 528, 1005, 1034, 1348
PspPI GGNCC 7 cut(s) 184, 260, 265, 293, 324, 349, 1032
PspXI VCTCGAGB 1 cut(s) 123
PstI CTGCAG 3 cut(s) 241, 1149, 1345
PstNI CAGNNNCTG 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 2 cut(s) 140, 722
RsaNI GTAC 2 cut(s) 139, 721
Rsr2I CGGWCCG 1 cut(s) 260
RsrII CGGWCCG 1 cut(s) 260
SacI GAGCTC 2 cut(s) 1218, 1242
SacII CCGCGG 1 cut(s) 255
SalI GTCGAC 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 1247
SaqAI TTAA 1 cut(s) 447
Sau96I GGNCC 7 cut(s) 184, 260, 265, 293, 324, 349, 1032
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 4 cut(s) 115, 214, 443, 936
ScrFI CCNGG 5 cut(s) 290, 291, 523, 536, 879
SduI GDGCHC 2 cut(s) 1218, 1242
SfaNI GCATC 6 cut(s) 84, 612, 648, 876, 876, 1075
SfcI CTRYAG 4 cut(s) 237, 1145, 1341, 1364
SfiI GGCCNNNNNGGCC 1 cut(s) 254
Sfr274I CTCGAG 1 cut(s) 123
Sfr303I CCGCGG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 5 cut(s) 184, 260, 265, 293, 1032
SlaI CTCGAG 1 cut(s) 123
SmaI CCCGGG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 123
SmoI CTYRAG 1 cut(s) 123
Sse9I AATT 5 cut(s) 343, 474, 696, 738, 1318
SseBI AGGCCT 1 cut(s) 248
SspI AATATT 1 cut(s) 1192
SspMI CTAG 1 cut(s) 192
SstI GAGCTC 2 cut(s) 1218, 1242
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 5 cut(s) 288, 289, 521, 534, 877
StyI CCWWGG 2 cut(s) 327, 1330
TaaI ACNGT 3 cut(s) 213, 616, 823
TaiI ACGT 5 cut(s) 363, 410, 553, 864, 1031
TaqII GACCGA 1 cut(s) 805
TasI AATT 5 cut(s) 343, 474, 696, 738, 1318
TatI WGTACW 2 cut(s) 138, 720
TauI GCSGC 6 cut(s) 230, 233, 257, 354, 532, 784
TfiI GAWTC 3 cut(s) 25, 179, 564
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 2 cut(s) 644, 990
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 9 cut(s) 157, 224, 233, 236, 239, 463, 764, 1144, 1340
Tsp45I GTSAC 1 cut(s) 817
TspGWI ACGGA 2 cut(s) 217, 803
TspMI CCCGGG 1 cut(s) 289
TspRI CASTG 2 cut(s) 644, 990
Van91I CCANNNNNTGG 3 cut(s) 333, 493, 583
VpaK11BI GGWCC 5 cut(s) 184, 260, 265, 293, 1032
XapI RAATTY 2 cut(s) 343, 696
XcmI CCANNNNNNNNNTGG 1 cut(s) 990
XhoI CTCGAG 1 cut(s) 123
XmaI CCCGGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 1 cut(s) 192
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.