Rmu_ssc0000369.1_g000007

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000369.1
OG0015894
Physical Location & Seq
Reverse (-)
31416 .. 31787
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000369.1_g000007.1.cds

Sequence Viewer

Length: 372 bp
atggattgggttttggttccctacacccaccagaatttgacatggacacaacacgccttcaatgaaaagattggggacattcaaggagttgctaaagatgcatttgctaagctcaaagggaggtggtcttgtttacagaagaggactgaggtgaagctccaggacctgcctgtggtgcttggggcttgctgtgttctccacaacatttgcacaatgaggaacgatgagcttgatcccgagctcaaatacgatctctttgatgatgagatggtttctgaaaatggtctgaggtctgctggtgcagttcaagctagggatcacattgctcataacttgcttcaccatggccttgcaggcactggatttcattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

123

Amino Acids

13.88

Weight (kDa)

6.02

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29780
fragaria_vesca FvH4_1g07981
malus_domestica MD15G1211600.v1.1
prunus_persica Prupe.7G205200_v2.0.a1
pyrus_communis pycom02g06720
rosa_chinensis RchiOBHm_Chr2g0094021
rosa_laevigata RLG00000016446
rosa_multiflora Rmu_co8489597.1_g000001 Rmu_ssc0000369.1_g000007
rosa_roxburghii Rroxscaffold_2G00147550
rosa_rugosa Rorug02G0040400
rosa_samantha Rh2AG087800 Rh2BG088000 Rh2CG090300 Rh2DG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AclWI GGATC 2 cut(s) 227, 324
AcsI RAATTY 1 cut(s) 34
AfiI CCNNNNNNNGG 1 cut(s) 172
AgsI TTSAA 3 cut(s) 61, 83, 308
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 5 cut(s) 112, 157, 229, 241, 311
AluI AGCT 5 cut(s) 112, 157, 229, 241, 311
Alw21I GWGCWC 1 cut(s) 243
AlwI GGATC 2 cut(s) 227, 324
AlwNI CAGNNNCTG 2 cut(s) 166, 359
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 163, 332
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BccI CCATC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 161
BfaI CTAG 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 354
BlpI GCTNAGC 1 cut(s) 108
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 88
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 343
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 364
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 138, 278
BseNI ACTGG 1 cut(s) 364
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 348
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 3 cut(s) 232, 250, 316
Bsp1720I GCTNAGC 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 343
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 2 cut(s) 139, 279
BspLI GGNNCC 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 227, 324
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 3 cut(s) 232, 250, 316
BssT1I CCWWGG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 161
Bst6I CTCTTC 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 187, 355
BstDEI CTNAG 3 cut(s) 108, 147, 287
BstDSI CCRYGG 1 cut(s) 343
BstKTI GATC 3 cut(s) 235, 253, 319
BstMBI GATC 3 cut(s) 232, 250, 316
BstMWI GCNNNNNNNGC 4 cut(s) 98, 175, 308, 354
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 187, 355
CaiI CAGNNNCTG 2 cut(s) 166, 359
Cfr13I GGNCC 1 cut(s) 163
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 2 cut(s) 42, 344
CviJI RGCY 7 cut(s) 112, 157, 185, 229, 241, 311, 348
CviKI_1 RGCY 7 cut(s) 112, 157, 185, 229, 241, 311, 348
DdeI CTNAG 3 cut(s) 108, 147, 287
DpnI GATC 3 cut(s) 234, 252, 318
DpnII GATC 3 cut(s) 232, 250, 316
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 343
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 343
FaeI CATG 2 cut(s) 45, 347
FaiI YATR 3 cut(s) 43, 330, 345
FaqI GGGAC 1 cut(s) 89
FatI CATG 2 cut(s) 41, 343
FriOI GRGCYC 1 cut(s) 243
FspBI CTAG 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 1 cut(s) 348
Hin1II CATG 2 cut(s) 45, 347
HphI GGTGA 2 cut(s) 163, 332
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 2 cut(s) 277, 288
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 67
HpyCH4V TGCA 4 cut(s) 101, 210, 302, 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 175, 308, 354
HpyF3I CTNAG 3 cut(s) 108, 147, 287
Hsp92II CATG 2 cut(s) 45, 347
Kzo9I GATC 3 cut(s) 232, 250, 316
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 8 cut(s) 44, 146, 173, 179, 183, 282, 339, 345
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 312
MalI GATC 3 cut(s) 234, 252, 318
MboI GATC 3 cut(s) 232, 250, 316
MboII GAAGA 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 1 cut(s) 34
MnlI CCTC 5 cut(s) 114, 135, 142, 210, 282
Mph1103I ATGCAT 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 98, 175, 308, 354
NcoI CCATGG 1 cut(s) 343
NdeII GATC 3 cut(s) 232, 250, 316
NlaIII CATG 2 cut(s) 45, 347
NlaIV GGNNCC 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
PstNI CAGNNNCTG 2 cut(s) 166, 359
SacI GAGCTC 1 cut(s) 243
Sau3AI GATC 3 cut(s) 232, 250, 316
Sau96I GGNCC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 243
SetI ASST 9 cut(s) 114, 125, 153, 159, 168, 231, 243, 293, 313
SfaNI GCATC 1 cut(s) 88
SinI GGWCC 1 cut(s) 163
Sse9I AATT 1 cut(s) 34
SspMI CTAG 1 cut(s) 312
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 343
TasI AATT 1 cut(s) 34
TscAI CASTG 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 78, 356
TspRI CASTG 1 cut(s) 364
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 34
XspI CTAG 1 cut(s) 312
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.