AT4G30950

Omega-6 fatty acid desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016050
Physical Location & Seq
Reverse (-)
15056975 .. 15059846
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30950.1

Sequence Viewer

Length: 1347 bp
ATGGCTTCCAGAATTGCTGATTCTCTCTTCGCCTTCACGGGCCCACAGCAATGTCTTCCTAGGGTTCCTAAGCTTGCTGCTTCTTCTGCTCGTGTTTCTCCTGGTGTATATGCTGTGAAGCCGATTGATCTTCTGTTAAAAGGACGAACTCATCGAAGTAGAAGATGTGTAGCTCCTGTGAAAAGGAGAATTGGATGTATCAAAGCGGTGGCTGCTCCAGTTGCACCGCCTTCAGCTGACAGTGCAGAAGACAGGGAACAGTTAGCAGAAAGCTATGGATTCAGACAAATTGGAGAAGATCTTCCTGAGAATGTCACCTTAAAAGATATCATGGATACACTTCCCAAAGAGGTGTTTGAGATTGATGATCTGAAAGCTTTGAAGTCTGTGTTGATATCTGTGACTTCATACACTTTGGGGCTCTTCATGATTGCAAAATCGCCGTGGTATCTGCTACCGTTGGCTTGGGCATGGACAGGAACTGCAATTACCGGGTTCTTTGTGATAGGTCATGATTGTGCACATAAGTCATTTTCAAAGAACAAATTGGTGGAAGACATTGTGGGTACTCTCGCCTTCCTACCACTTGTCTACCCATATGAGCCATGGCGGTTTAAGCACGACCGCCATCACGCCAAAACCAACATGTTAGTTCATGACACAGCTTGGCAGCCAGTTCCGCCAGAGGAGTTTGAGTCATCACCCGTGATGAGAAAGGCAATCATTTTTGGATATGGCCCAATTAGACCTTGGTTGTCCATAGCTCACTGGGTGAACTGGCACTTCAATCTGAAAAAGTTCAGAGCGAGCGAGGTGAATAGGGTGAAGATAAGTTTGGCTTGTGTTTTCGCCTTCATGGCCGTTGGGTGGCCACTGATCGTATACAAAGTTGGTATATTGGGATGGGTAAAATTCTGGTTAATGCCATGGTTGGGCTATCACTTCTGGATGAGCACATTCACAATGGTTCATCATACGGCTCCGCATATACCTTTCAAGCCTGCGGATGAGTGGAACGCGGCTCAGGCCCAGCTGAATGGAACTGTTCATTGTGACTACCCTAGTTGGATTGAAATTCTCTGCCATGATATCAACGTTCACATCCCGCATCATATTAGCCCAAGAATACCGAGCTACAATCTCCGTGCAGCTCATGAGTCTATACAAGAGAACTGGGGAAAGTATACAAACTTGGCTACATGGAACTGGCGATTGATGAAGACGATAATGACTGTGTGTCATGTCTATGACAAAGAGGAGAACTACATTCCTTTTGACCGGTTAGCCCCTGAAGAATCTCAGCCAATAACCTTCCTCAAGAAAGCAATGCCTAACTACACAGCCTGA

Protein Analysis

448

Amino Acids

51.22

Weight (kDa)

9.01

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 148 - 395 2.4e-31 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30950
fragaria_vesca FvH4_1g01030
malus_domestica MD15G1152700.v1.1
prunus_persica Prupe.7G261900_v2.0.a1
pyrus_communis pycom15g13730
rosa_chinensis RchiOBHm_Chr2g0085771
rosa_laevigata RLG00000015718
rosa_multiflora Rmu_sc0009759.1_g000002
rosa_roxburghii Rroxscaffold_2G00155050
rosa_rugosa Rorug01G0463000
rosa_samantha Rh2AG012300 Rh2BG012900 Rh2CG013600 Rh2DG014600
rosa_wichuraiana Rw2G001090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 591, 882, 1184
AccII CGCG 1 cut(s) 1019
AciI CCGC 9 cut(s) 206, 227, 610, 625, 680, 983, 1004, 1019, 1106
AclI AACGTT 1 cut(s) 1095
AcoI YGGCCR 2 cut(s) 858, 869
AcsI RAATTY 2 cut(s) 911, 1074
AcuI CTGAAG 2 cut(s) 216, 1311
AdeI CACNNNGTG 1 cut(s) 772
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 2 cut(s) 867, 932
AflIII ACRYGT 1 cut(s) 645
AgeI ACCGGT 1 cut(s) 1278
AgsI TTSAA 5 cut(s) 382, 537, 787, 997, 1073
AhdI GACNNNNNGTC 1 cut(s) 1236
AjnI CCWGG 1 cut(s) 100
AjuI GAANNNNNNNTTGG 2 cut(s) 818, 850
Alw21I GWGCWC 2 cut(s) 523, 956
Alw44I GTGCAC 1 cut(s) 519
AlwNI CAGNNNCTG 1 cut(s) 482
AoxI GGCC 5 cut(s) 40, 736, 858, 869, 1026
ApaI GGGCCC 1 cut(s) 44
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 4 cut(s) 77, 212, 670, 1148
ApoI RAATTY 2 cut(s) 911, 1074
AsiGI ACCGGT 1 cut(s) 1278
Asp700I GAANNNNTTC 2 cut(s) 300, 797
AspA2I CCTAGG 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 40, 41, 737, 1027
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 5 cut(s) 307, 693, 784, 826, 835
AvrII CCTAGG 1 cut(s) 59
BaeGI GKGCMC 2 cut(s) 44, 523
BalI TGGCCA 1 cut(s) 871
BanII GRGCYC 2 cut(s) 44, 423
BauI CACGAG 1 cut(s) 90
BbsI GAAGAC 4 cut(s) 47, 255, 561, 1226
Bbv12I GWGCWC 2 cut(s) 523, 956
BbvI GCAGC 4 cut(s) 64, 199, 682, 1160
BccI CCATC 2 cut(s) 636, 897
BceAI ACGGC 3 cut(s) 427, 845, 993
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 328
BcnI CCSGG 1 cut(s) 493
BfaI CTAG 2 cut(s) 60, 1062
BfuI GTATCC 1 cut(s) 328
BglI GCCNNNNNGGC 1 cut(s) 857
BglII AGATCT 1 cut(s) 298
BisI GCNGC 5 cut(s) 78, 213, 671, 1020, 1149
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 5 cut(s) 79, 214, 672, 1021, 1150
Bme1390I CCNGG 2 cut(s) 102, 493
BmeRI GACNNNNNGTC 1 cut(s) 1236
BmgT120I GGNCC 4 cut(s) 40, 41, 737, 1027
BmiI GGNNCC 3 cut(s) 42, 66, 981
BmrFI CCNGG 2 cut(s) 102, 493
BmrI ACTGGG 2 cut(s) 778, 1183
BmsI GCATC 1 cut(s) 1117
BmuI ACTGGG 2 cut(s) 778, 1183
BpiI GAAGAC 4 cut(s) 47, 255, 561, 1226
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 2 cut(s) 69, 1023
BpuEI CTTGAG 1 cut(s) 1301
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 5 cut(s) 59, 443, 605, 749, 926
BsaWI WCCGGW 1 cut(s) 1278
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bsc4I CCNNNNNNNGG 2 cut(s) 867, 932
Bse118I RCCGGY 1 cut(s) 1278
Bse1I ACTGG 6 cut(s) 218, 674, 773, 782, 1178, 1211
Bse3DI GCAATG 2 cut(s) 56, 1332
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 5 cut(s) 59, 443, 605, 749, 926
BseGI GGATG 5 cut(s) 200, 908, 954, 1012, 1101
BseLI CCNNNNNNNGG 2 cut(s) 867, 932
BseMI GCAATG 2 cut(s) 56, 1332
BseMII CTCAG 3 cut(s) 297, 1037, 1313
BseNI ACTGG 6 cut(s) 218, 674, 773, 782, 1178, 1211
BseRI GAGGAG 2 cut(s) 701, 1271
BseSI GKGCMC 2 cut(s) 44, 523
BseXI GCAGC 4 cut(s) 64, 199, 682, 1160
BseYI CCCAGC 1 cut(s) 1029
BsgI GTGCAG 2 cut(s) 264, 1167
Bsh1236I CGCG 1 cut(s) 1019
Bsh1285I CGRYCG 1 cut(s) 625
BshFI GGCC 5 cut(s) 42, 738, 860, 871, 1028
BshTI ACCGGT 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 625
BsiHKAI GWGCWC 2 cut(s) 523, 956
BsiSI CCGG 2 cut(s) 492, 1279
BslI CCNNNNNNNGG 2 cut(s) 867, 932
BsnI GGCC 5 cut(s) 42, 738, 860, 871, 1028
Bsp120I GGGCCC 1 cut(s) 40
Bsp1286I GDGCHC 4 cut(s) 44, 423, 523, 956
Bsp143I GATC 4 cut(s) 127, 298, 367, 876
Bsp19I CCATGG 2 cut(s) 605, 926
BspACI CCGC 9 cut(s) 206, 227, 610, 625, 680, 983, 1004, 1019, 1106
BspANI GGCC 5 cut(s) 42, 738, 860, 871, 1028
BspCNI CTCAG 3 cut(s) 298, 1036, 1312
BspFNI CGCG 1 cut(s) 1019
BspHI TCATGA 4 cut(s) 426, 511, 655, 1153
BspLI GGNNCC 3 cut(s) 42, 66, 981
BspQI GCTCTTC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 56, 1332
BsrFI RCCGGY 1 cut(s) 1278
BsrI ACTGG 6 cut(s) 218, 674, 773, 782, 1178, 1211
BssAI RCCGGY 1 cut(s) 1278
BssECI CCNNGG 5 cut(s) 59, 443, 605, 749, 926
BssMI GATC 4 cut(s) 127, 298, 367, 876
BssNAI GTATAC 2 cut(s) 883, 1185
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 4 cut(s) 59, 605, 749, 926
Bst1107I GTATAC 2 cut(s) 883, 1185
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 5 cut(s) 242, 261, 459, 1045, 1234
Bst6I CTCTTC 2 cut(s) 32, 428
BstC8I GCNNGC 3 cut(s) 75, 808, 1002
BstDEI CTNAG 4 cut(s) 69, 306, 1023, 1299
BstDSI CCRYGG 3 cut(s) 443, 605, 926
BstF5I GGATG 5 cut(s) 200, 908, 954, 1012, 1101
BstFNI CGCG 1 cut(s) 1019
BstKTI GATC 4 cut(s) 130, 301, 370, 879
BstMBI GATC 4 cut(s) 127, 298, 367, 876
BstMCI CGRYCG 1 cut(s) 625
BstMWI GCNNNNNNNGC 8 cut(s) 86, 212, 221, 242, 616, 679, 857, 1025
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 2 cut(s) 100, 491
BstSLI GKGCMC 2 cut(s) 44, 523
BstUI CGCG 1 cut(s) 1019
BstV1I GCAGC 4 cut(s) 64, 199, 682, 1160
BstV2I GAAGAC 4 cut(s) 47, 255, 561, 1226
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 1 cut(s) 298
BstZ17I GTATAC 2 cut(s) 883, 1185
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 5 cut(s) 42, 738, 860, 871, 1028
BtgI CCRYGG 3 cut(s) 443, 605, 926
BtsCI GGATG 5 cut(s) 200, 908, 954, 1012, 1101
BtsIMutI CAGTG 3 cut(s) 247, 766, 872
Cac8I GCNNGC 3 cut(s) 75, 808, 1002
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 4 cut(s) 426, 511, 655, 1153
Cfr10I RCCGGY 1 cut(s) 1278
Cfr13I GGNCC 4 cut(s) 40, 41, 737, 1027
Csp6I GTAC 1 cut(s) 567
CspAI ACCGGT 1 cut(s) 1278
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 4 cut(s) 69, 306, 1023, 1299
DpnI GATC 4 cut(s) 129, 300, 369, 878
DpnII GATC 4 cut(s) 127, 298, 367, 876
DraIII CACNNNGTG 1 cut(s) 772
DriI GACNNNNNGTC 1 cut(s) 1236
EaeI YGGCCR 2 cut(s) 858, 869
Eam1104I CTCTTC 2 cut(s) 32, 428
Eam1105I GACNNNNNGTC 1 cut(s) 1236
EarI CTCTTC 2 cut(s) 32, 428
EciI GGCGGA 1 cut(s) 669
Eco130I CCWWGG 4 cut(s) 59, 605, 749, 926
Eco24I GRGCYC 2 cut(s) 44, 423
Eco32I GATATC 3 cut(s) 328, 396, 1090
Eco57I CTGAAG 2 cut(s) 216, 1311
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 3 cut(s) 328, 396, 1090
EcoT14I CCWWGG 4 cut(s) 59, 605, 749, 926
EcoT38I GRGCYC 2 cut(s) 44, 423
ErhI CCWWGG 4 cut(s) 59, 605, 749, 926
FalI AAGNNNNNCTT 2 cut(s) 823, 855
FauI CCCGC 1 cut(s) 1113
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 3 cut(s) 591, 882, 1184
Fnu4HI GCNGC 5 cut(s) 78, 213, 671, 1020, 1149
FokI GGATG 5 cut(s) 207, 915, 961, 1019, 1088
FriOI GRGCYC 2 cut(s) 44, 423
Fsp4HI GCNGC 5 cut(s) 78, 213, 671, 1020, 1149
FspBI CTAG 2 cut(s) 60, 1062
GluI GCNGC 5 cut(s) 78, 213, 671, 1020, 1149
GsaI CCCAGC 1 cut(s) 1033
GsuI CTGGAG 1 cut(s) 201
HaeIII GGCC 5 cut(s) 42, 738, 860, 871, 1028
HapII CCGG 2 cut(s) 492, 1279
HindIII AAGCTT 2 cut(s) 71, 375
HinfI GANTC 5 cut(s) 20, 279, 695, 1157, 1295
HpaII CCGG 2 cut(s) 492, 1279
HphI GGTGA 5 cut(s) 307, 693, 784, 826, 835
Hpy166II GTNNAC 6 cut(s) 521, 592, 775, 883, 1099, 1185
Hpy188I TCNGA 4 cut(s) 284, 372, 792, 803
Hpy188III TCNNGA 8 cut(s) 9, 305, 427, 512, 656, 946, 1154, 1318
Hpy8I GTNNAC 6 cut(s) 521, 592, 775, 883, 1099, 1185
HpyAV CCTTC 5 cut(s) 43, 240, 586, 862, 1321
HpyCH4III ACNGT 5 cut(s) 242, 261, 459, 1045, 1234
HpyCH4IV ACGT 1 cut(s) 1095
HpyCH4V TGCA 6 cut(s) 224, 245, 434, 485, 521, 1148
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 212, 221, 242, 616, 679, 857, 1025
HpyF3I CTNAG 4 cut(s) 69, 306, 1023, 1299
HpySE526I ACGT 1 cut(s) 1095
Kzo9I GATC 4 cut(s) 127, 298, 367, 876
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 3 cut(s) 178, 220, 985
Lsp1109I GCAGC 4 cut(s) 64, 199, 682, 1160
LweI GCATC 1 cut(s) 1117
MaeI CTAG 2 cut(s) 60, 1062
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 3 cut(s) 313, 400, 1052
MalI GATC 4 cut(s) 129, 300, 369, 878
MboI GATC 4 cut(s) 127, 298, 367, 876
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 4 cut(s) 44, 423, 523, 956
MlsI TGGCCA 1 cut(s) 871
MluCI AATT 8 cut(s) 12, 189, 288, 486, 545, 741, 911, 1074
MluNI TGGCCA 1 cut(s) 871
MlyI GAGTC 2 cut(s) 704, 1166
MmeI TCCRAC 1 cut(s) 1046
MnlI CCTC 5 cut(s) 343, 679, 805, 1249, 1325
Mox20I TGGCCA 1 cut(s) 871
MroXI GAANNNNTTC 2 cut(s) 300, 797
MscI TGGCCA 1 cut(s) 871
MseI TTAA 4 cut(s) 137, 320, 615, 920
MslI CAYNNNNRTG 3 cut(s) 49, 516, 1245
Msp20I TGGCCA 1 cut(s) 871
MspA1I CMGCKG 2 cut(s) 236, 1033
MspI CCGG 2 cut(s) 492, 1279
MspR9I CCNGG 2 cut(s) 102, 493
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 1019
MwoI GCNNNNNNNGC 8 cut(s) 86, 212, 221, 242, 616, 679, 857, 1025
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 2 cut(s) 605, 926
NdeI CATATG 1 cut(s) 598
NdeII GATC 4 cut(s) 127, 298, 367, 876
NlaIV GGNNCC 3 cut(s) 42, 66, 981
NmuCI GTSAC 3 cut(s) 313, 400, 1052
NspI RCATGY 1 cut(s) 649
PagI TCATGA 4 cut(s) 426, 511, 655, 1153
PciI ACATGT 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 300, 797
PfeI GAWTC 3 cut(s) 20, 279, 1295
PinAI ACCGGT 1 cut(s) 1278
PkrI GCNGC 5 cut(s) 79, 214, 672, 1021, 1150
PleI GAGTC 2 cut(s) 703, 1165
PpsI GAGTC 2 cut(s) 703, 1165
PscI ACATGT 1 cut(s) 645
Psp1406I AACGTT 1 cut(s) 1095
Psp6I CCWGG 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 1029
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 42, 66, 981
PspOMI GGGCCC 1 cut(s) 40
PspPI GGNCC 4 cut(s) 40, 41, 737, 1027
PsrI GAACNNNNNNTAC 2 cut(s) 472, 504
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 1 cut(s) 298
PvuII CAGCTG 2 cut(s) 236, 1033
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 3 cut(s) 49, 516, 1245
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 4 cut(s) 137, 320, 615, 920
SatI GCNGC 5 cut(s) 78, 213, 671, 1020, 1149
Sau3AI GATC 4 cut(s) 127, 298, 367, 876
Sau96I GGNCC 4 cut(s) 40, 41, 737, 1027
SchI GAGTC 2 cut(s) 704, 1166
ScrFI CCNGG 2 cut(s) 102, 493
SduI GDGCHC 4 cut(s) 44, 423, 523, 956
SfaNI GCATC 1 cut(s) 1117
SmiMI CAYNNNNRTG 3 cut(s) 49, 516, 1245
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
Sse9I AATT 8 cut(s) 12, 189, 288, 486, 545, 741, 911, 1074
SsiI CCGC 9 cut(s) 206, 227, 610, 625, 680, 983, 1004, 1019, 1106
SspMI CTAG 2 cut(s) 60, 1062
StyD4I CCNGG 2 cut(s) 100, 491
StyI CCWWGG 4 cut(s) 59, 605, 749, 926
TaaI ACNGT 5 cut(s) 242, 261, 459, 1045, 1234
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 1 cut(s) 154
TasI AATT 8 cut(s) 12, 189, 288, 486, 545, 741, 911, 1074
TauI GCSGC 1 cut(s) 1022
TfiI GAWTC 3 cut(s) 20, 279, 1295
Tru1I TTAA 4 cut(s) 137, 320, 615, 920
Tru9I TTAA 4 cut(s) 137, 320, 615, 920
TscAI CASTG 3 cut(s) 247, 773, 879
TseFI GTSAC 3 cut(s) 313, 400, 1052
TseI GCWGC 4 cut(s) 77, 212, 670, 1148
Tsp45I GTSAC 3 cut(s) 313, 400, 1052
TspDTI ATGAA 7 cut(s) 396, 415, 644, 844, 959, 1037, 1232
TspGWI ACGGA 1 cut(s) 1133
TspRI CASTG 3 cut(s) 247, 773, 879
VneI GTGCAC 1 cut(s) 519
XapI RAATTY 2 cut(s) 911, 1074
XceI RCATGY 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 2 cut(s) 603, 747
XmaJI CCTAGG 1 cut(s) 59
XmiI GTMKAC 3 cut(s) 591, 882, 1184
XmnI GAANNNNTTC 2 cut(s) 300, 797
XspI CTAG 2 cut(s) 60, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.