Rroxscaffold_2G00155050

Omega-6 fatty acid desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016050
Physical Location & Seq
Forward (+)
91470621 .. 91475183
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155050.1

Sequence Viewer

Length: 1110 bp
ATGCATCGGGGCTCCAACATCGGATTGGGACCGAGAGAAGGAGATCATGATTCTATGTTACTTTCCAAGGGCCCCTATCATAAGCCAATTCGAAGCCAGAGAGTTGGAGCCCATTTTTCTCCAGGAATCTGCCATTTGAAATGGGACAACCATCTCCAAAAGGGTATCAAACATCAACAGTGTTCTCCTAGAAATCGGAGGACCTTTGTACAAGCAATTGCCATTCCAGTTGCACCATCTTCACCAGACAGTGCTGAGTACAGAAAACAATTATCTGAAAGTTATGGTTTCAAACAAATTGGAGAACCACTCCCGGACAATGTTACGCTAAAGGATGTCATTGATTCCCTTCCAAAAAAGGTGTTTGAGATTGATGATGTGAAAGCATGGAAGTCAGTTCTAATATCTGCCACTTCCTACGCAATTGGGCTATTTTTGATTTCAAAAGCCCCATGGTTGGTTGAAGATACAGCTTGGCAGCCTATTCCGAAAGAAGAGTTTGATTCATCCCCTTTGTTGCGAAAGGCAATTATTTATGGATATGGCCCTTTGCGTCCTTGGATGTCAATAGCTCACTGGTGGCTCGTCCACTTCAATTTGAAGAAGTTCAGAACAAGTGAAGTTAAAAGGGCTGCTATAAGTTTGGCTTGTGTTTTTGCGTTTATGGGAATTGGATGGCCTCTAATTGTGTACAAGACGGGGATCATGGGATGGATGAGTACATTCACAATGGTTCATCATACGGCCCCTCATATACCTTTCAAATCTGCGGATGAGTGGAATGCTGCTCAGGCTCAGATTAACGGAACAGTTCATTGTGACTACCCCTCATGGATTGAGATCCTCTGCCATGATATTAATGTCCATATACCCCATCATATATCTCCAAGGATTCCAAGCTATAACTTACGAGCAGCTCAGAAGTCTATCCAAGAAAACTGGGGAAAGTATCTGAACGAGGCTACATGGAATTGGCGCTTGATGAAGACGATAATGACAGTGTGCCATGTTTACAACAAGGAGGAGAACTACATTCCATTTGACGAACTTGCCCCTGAAGAATCTCTTCCGATTGCATTCCTCAAAAAAGTGATGCCTGATTATGCTTGA

Protein Analysis

369

Amino Acids

42.22

Weight (kDa)

8.95

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 187 - 318 5.9e-13 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30950
fragaria_vesca FvH4_1g01030
malus_domestica MD15G1152700.v1.1
prunus_persica Prupe.7G261900_v2.0.a1
pyrus_communis pycom15g13730
rosa_chinensis RchiOBHm_Chr2g0085771
rosa_laevigata RLG00000015718
rosa_multiflora Rmu_sc0009759.1_g000002
rosa_roxburghii Rroxscaffold_2G00155050
rosa_rugosa Rorug01G0463000
rosa_samantha Rh2AG012300 Rh2BG012900 Rh2CG013600 Rh2DG014600
rosa_wichuraiana Rw2G001090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 770
AclWI GGATC 2 cut(s) 710, 835
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 4 cut(s) 210, 260, 692, 721
AfiI CCNNNNNNNGG 2 cut(s) 38, 457
AgsI TTSAA 7 cut(s) 139, 292, 444, 464, 595, 601, 763
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 4 cut(s) 473, 572, 900, 917
AluI AGCT 4 cut(s) 473, 572, 900, 917
AlwI GGATC 2 cut(s) 710, 835
AoxI GGCC 4 cut(s) 70, 544, 677, 744
ApaI GGGCCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 478, 632, 785, 914
AseI ATTAAT 1 cut(s) 858
Asp700I GAANNNNTTC 2 cut(s) 605, 1065
AspLEI GCGC 1 cut(s) 978
AspS9I GGNCC 6 cut(s) 29, 70, 71, 201, 545, 745
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 234
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 2 cut(s) 29, 201
BaeGI GKGCMC 1 cut(s) 74
BanII GRGCYC 3 cut(s) 14, 74, 112
BbsI GAAGAC 1 cut(s) 992
BbvI GCAGC 4 cut(s) 490, 619, 772, 926
BccI CCATC 5 cut(s) 159, 244, 669, 705, 882
BceAI ACGGC 1 cut(s) 759
BciT130I CCWGG 1 cut(s) 123
BcnI CCSGG 1 cut(s) 314
BfaI CTAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 979
BisI GCNGC 4 cut(s) 479, 633, 786, 915
BlsI GCNGC 4 cut(s) 480, 634, 787, 916
Bme1390I CCNGG 2 cut(s) 123, 314
Bme18I GGWCC 2 cut(s) 29, 201
BmgT120I GGNCC 6 cut(s) 29, 70, 71, 201, 545, 745
BmiI GGNNCC 6 cut(s) 13, 30, 72, 73, 109, 747
BmrFI CCNGG 2 cut(s) 123, 314
BmrI ACTGGG 1 cut(s) 949
BmsI GCATC 2 cut(s) 13, 1083
BmuI ACTGGG 1 cut(s) 949
BpiI GAAGAC 1 cut(s) 992
BplI GAGNNNNNCTC 2 cut(s) 294, 326
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 66, 452, 557, 887
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 457
Bse1I ACTGG 3 cut(s) 227, 581, 944
Bse8I GATNNNNATC 1 cut(s) 839
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 66, 452, 557, 887
BseGI GGATG 7 cut(s) 340, 506, 567, 680, 716, 720, 778
BseJI GATNNNNATC 1 cut(s) 839
BseLI CCNNNNNNNGG 2 cut(s) 38, 457
BseMII CTCAG 4 cut(s) 246, 803, 809, 932
BseNI ACTGG 3 cut(s) 227, 581, 944
BseRI GAGGAG 1 cut(s) 1037
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 4 cut(s) 490, 619, 772, 926
BshFI GGCC 4 cut(s) 72, 546, 679, 746
BsiSI CCGG 1 cut(s) 314
BslFI GGGAC 2 cut(s) 42, 158
BslI CCNNNNNNNGG 2 cut(s) 38, 457
BsmFI GGGAC 2 cut(s) 42, 158
BsmI GAATGC 2 cut(s) 787, 1076
BsnI GGCC 4 cut(s) 72, 546, 679, 746
Bsp119I TTCGAA 1 cut(s) 91
Bsp120I GGGCCC 1 cut(s) 70
Bsp1286I GDGCHC 3 cut(s) 14, 74, 112
Bsp1407I TGTACA 2 cut(s) 208, 690
Bsp143I GATC 3 cut(s) 43, 702, 840
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 1 cut(s) 770
BspANI GGCC 4 cut(s) 72, 546, 679, 746
BspCNI CTCAG 4 cut(s) 247, 802, 808, 931
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 6 cut(s) 13, 30, 72, 73, 109, 747
BspPI GGATC 2 cut(s) 710, 835
BspT104I TTCGAA 1 cut(s) 91
BsrGI TGTACA 2 cut(s) 208, 690
BsrI ACTGG 3 cut(s) 227, 581, 944
BssECI CCNNGG 4 cut(s) 66, 452, 557, 887
BssMI GATC 3 cut(s) 43, 702, 840
BssT1I CCWWGG 4 cut(s) 66, 452, 557, 887
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 4 cut(s) 180, 251, 811, 1000
Bst6I CTCTTC 2 cut(s) 489, 1071
BstAUI TGTACA 2 cut(s) 208, 690
BstBI TTCGAA 1 cut(s) 91
BstDEI CTNAG 4 cut(s) 255, 789, 795, 918
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 7 cut(s) 340, 506, 567, 680, 716, 720, 778
BstH2I RGCGCY 1 cut(s) 979
BstHHI GCGC 1 cut(s) 978
BstKTI GATC 3 cut(s) 46, 705, 843
BstMBI GATC 3 cut(s) 43, 702, 840
BstMWI GCNNNNNNNGC 1 cut(s) 791
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 121, 312
BstSLI GKGCMC 1 cut(s) 74
BstV1I GCAGC 4 cut(s) 490, 619, 772, 926
BstV2I GAAGAC 1 cut(s) 992
BstX2I RGATCY 1 cut(s) 840
BstXI CCANNNNNNTGG 1 cut(s) 104
BstYI RGATCY 1 cut(s) 840
BsuRI GGCC 4 cut(s) 72, 546, 679, 746
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 7 cut(s) 340, 506, 567, 680, 716, 720, 778
BtsIMutI CAGTG 4 cut(s) 185, 256, 574, 1005
CciI TCATGA 1 cut(s) 46
CfoI GCGC 1 cut(s) 978
Cfr13I GGNCC 6 cut(s) 29, 70, 71, 201, 545, 745
CseI GACGC 1 cut(s) 542
Csp6I GTAC 4 cut(s) 209, 259, 691, 720
CviAII CATG 8 cut(s) 47, 387, 453, 706, 831, 851, 966, 1007
CviQI GTAC 4 cut(s) 209, 259, 691, 720
DdeI CTNAG 4 cut(s) 255, 789, 795, 918
DpnI GATC 3 cut(s) 45, 704, 842
DpnII GATC 3 cut(s) 43, 702, 840
Eam1104I CTCTTC 2 cut(s) 489, 1071
EarI CTCTTC 2 cut(s) 489, 1071
Eco130I CCWWGG 4 cut(s) 66, 452, 557, 887
Eco24I GRGCYC 3 cut(s) 14, 74, 112
Eco47I GGWCC 2 cut(s) 29, 201
Eco57I CTGAAG 1 cut(s) 1077
EcoO109I RGGNCCY 3 cut(s) 70, 71, 201
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 4 cut(s) 66, 452, 557, 887
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 14, 74, 112
ErhI CCWWGG 4 cut(s) 66, 452, 557, 887
FaeI CATG 8 cut(s) 50, 390, 456, 709, 834, 854, 969, 1010
FalI AAGNNNNNCTT 4 cut(s) 631, 663, 1050, 1082
FaqI GGGAC 2 cut(s) 42, 158
FatI CATG 8 cut(s) 46, 386, 452, 705, 830, 850, 965, 1006
Fnu4HI GCNGC 4 cut(s) 479, 633, 786, 915
FokI GGATG 7 cut(s) 347, 493, 574, 687, 723, 727, 785
FriOI GRGCYC 3 cut(s) 14, 74, 112
Fsp4HI GCNGC 4 cut(s) 479, 633, 786, 915
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 977
GluI GCNGC 4 cut(s) 479, 633, 786, 915
GsuI CTGGAG 1 cut(s) 105
HaeII RGCGCY 1 cut(s) 979
HaeIII GGCC 4 cut(s) 72, 546, 679, 746
HapII CCGG 1 cut(s) 314
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 978
Hin1II CATG 8 cut(s) 50, 390, 456, 709, 834, 854, 969, 1010
Hin6I GCGC 1 cut(s) 976
HinP1I GCGC 1 cut(s) 976
HinfI GANTC 6 cut(s) 50, 126, 344, 503, 892, 1061
HpaII CCGG 1 cut(s) 314
HphI GGTGA 1 cut(s) 234
Hpy166II GTNNAC 3 cut(s) 589, 691, 1012
Hpy188I TCNGA 9 cut(s) 23, 198, 277, 489, 611, 798, 921, 954, 1071
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 3 cut(s) 589, 691, 1012
HpyAV CCTTC 2 cut(s) 32, 359
HpyCH4III ACNGT 4 cut(s) 180, 251, 811, 1000
HpyCH4V TGCA 3 cut(s) 4, 233, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 791
HpyF3I CTNAG 4 cut(s) 255, 789, 795, 918
Hsp92II CATG 8 cut(s) 50, 390, 456, 709, 834, 854, 969, 1010
HspAI GCGC 1 cut(s) 976
Kzo9I GATC 3 cut(s) 43, 702, 840
LmnI GCTCC 2 cut(s) 17, 107
Lsp1109I GCAGC 4 cut(s) 490, 619, 772, 926
LweI GCATC 2 cut(s) 13, 1083
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 57, 322, 818
MalI GATC 3 cut(s) 45, 704, 842
MboI GATC 3 cut(s) 43, 702, 840
MboII GAAGA 7 cut(s) 231, 476, 506, 613, 997, 1058, 1070
MfeI CAATTG 2 cut(s) 216, 423
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 3 cut(s) 14, 74, 112
MmeI TCCRAC 2 cut(s) 39, 85
MnlI CCTC 8 cut(s) 192, 690, 759, 838, 854, 952, 1015, 1091
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 605, 1065
MseI TTAA 3 cut(s) 624, 801, 858
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 123, 314
MunI CAATTG 2 cut(s) 216, 423
Mva1269I GAATGC 2 cut(s) 787, 1076
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 791
NciI CCSGG 1 cut(s) 314
NcoI CCATGG 1 cut(s) 452
NdeII GATC 3 cut(s) 43, 702, 840
NlaIII CATG 8 cut(s) 50, 390, 456, 709, 834, 854, 969, 1010
NlaIV GGNNCC 6 cut(s) 13, 30, 72, 73, 109, 747
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 91
PagI TCATGA 1 cut(s) 46
PctI GAATGC 2 cut(s) 787, 1076
PdmI GAANNNNTTC 2 cut(s) 605, 1065
PfeI GAWTC 6 cut(s) 50, 126, 344, 503, 892, 1061
PfoI TCCNGGA 2 cut(s) 121, 312
PkrI GCNGC 4 cut(s) 480, 634, 787, 916
PpuMI RGGWCCY 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 858
Psp5II RGGWCCY 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 6 cut(s) 13, 30, 72, 73, 109, 747
PspOMI GGGCCC 1 cut(s) 70
PspPI GGNCC 6 cut(s) 29, 70, 71, 201, 545, 745
PspPPI RGGWCCY 1 cut(s) 201
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 4 cut(s) 210, 260, 692, 721
RsaNI GTAC 4 cut(s) 209, 259, 691, 720
SaqAI TTAA 3 cut(s) 624, 801, 858
SatI GCNGC 4 cut(s) 479, 633, 786, 915
Sau3AI GATC 3 cut(s) 43, 702, 840
Sau96I GGNCC 6 cut(s) 29, 70, 71, 201, 545, 745
ScrFI CCNGG 2 cut(s) 123, 314
SduI GDGCHC 3 cut(s) 14, 74, 112
SetI ASST 7 cut(s) 206, 363, 475, 574, 760, 902, 919
SfaNI GCATC 2 cut(s) 13, 1083
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 2 cut(s) 29, 201
SsiI CCGC 1 cut(s) 770
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 121, 312
StyI CCWWGG 4 cut(s) 66, 452, 557, 887
TaaI ACNGT 4 cut(s) 180, 251, 811, 1000
TaqI TCGA 1 cut(s) 91
TaqII GACCGA 1 cut(s) 46
TatI WGTACW 4 cut(s) 208, 258, 690, 719
TfiI GAWTC 6 cut(s) 50, 126, 344, 503, 892, 1061
Tru1I TTAA 3 cut(s) 624, 801, 858
Tru9I TTAA 3 cut(s) 624, 801, 858
TscAI CASTG 4 cut(s) 185, 256, 581, 1005
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 4 cut(s) 478, 632, 785, 914
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 4 cut(s) 495, 725, 803, 998
TspGWI ACGGA 1 cut(s) 819
TspRI CASTG 4 cut(s) 185, 256, 581, 1005
VpaK11BI GGWCC 2 cut(s) 29, 201
VspI ATTAAT 1 cut(s) 858
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmnI GAANNNNTTC 2 cut(s) 605, 1065
XspI CTAG 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.