AT4G32290

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015487
Physical Location & Seq
Reverse (-)
15589434 .. 15591213
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32290.1

Sequence Viewer

Length: 1155 bp
ATGATATCTCCGACGTTGTTTCCTCTCCTCTGCGCTTTATTTCTATGTCTTCCAGTGGCTGTTATCTTCACAGTCCAGCGAGAACTCACCTGCGTTATCTCGCCGGAGTTTGGTTTCCGCTCTCTTTCCGTCTTCTCTCTTTACTCTCGAAATGTCCCCGACTCTTCTTCTTCTTCTCCGGCGGTTCGGATTCGACAGCCGATTCCCAAAGAAGACGACCCTCTTCTTCGACTATCTTCTCGGGTTAACCCGAATCTACCTCCCGGTTCGACCCGGAAAATAGCTTTTATGTACTTAACGACGTCGCCTCTTCCGTTCGCGCCTCTTTGGGAGATGTTCTTCGACGGAATCTCCAAGAATCTTTACAATGTCTACGTTCACGCGGATCCGACCCGAGAATACGACCCGCCGTTCTCCGGCGTGTTCTTGAACCGGGTCATTCACTCTAAACCCTCGCTGAGACACACTCCCACTCTAACTGCGGCGGCGCGTAGACTACTAGCACACGCGCTTCTCGACGATCCATTGAATTACATGTTTGCGGTTATATCACCTTCATGCGTACCAATTCGCTCCTTTGACTTCACTTACAAGACATTAGTCTCGTCGCGCAAGAGCTTCATCGAGATACTTAAAGACGAGCCGTGGCAGTTCGATAGGTGGACGGCGATTGGGAGACACGCGATGCTTCCGGAGGTGAAGCTCGAGGAGTTTCGGATCGGGTCTCAGTTTTGGGTTTTGAAACGGAGACACGCGCGTGTGGTGGCGCGTGATCGGCGTATATGGGTGAAGTTTAACCAGACGTGCGTGCGGGAAGACTCGTGTTATCCTGAAGAAAGTTACTTTCCTACCCTTCTTAATATGCGGGATCCACGTGGATGTGTCCCCGCCACGCTTACTCACGTGGACTGGACTGTTAACGACGGTGGTCACCCGAGGATGTACGAGCCGGAGGAAGTCGTGCCTGAGCTGGTCTTGCGGCTTAGAAAGACTAGACCAAGATATGGTGAGGATGGAATCAACGGTTCGGAGTGGTCTAAGGTTGAGCGGATGGATCCGTTTCTGTTTGCTAGGAAGTTTTCACCGCAGGCTCTTGAGCCGTTGCTGGGTATGGCTCGAACCGTCTTGTTCAATGACTCAGCCGGTGGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

384

Amino Acids

44.04

Weight (kDa)

9.14

Isoelectric Point (pI)

58.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 94 - 328 5.2e-70 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 98
AatII GACGTC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 98
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccBSI CCGCTC 2 cut(s) 120, 1048
AccI GTMKAC 2 cut(s) 372, 493
AccII CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
AccIII TCCGGA 1 cut(s) 691
AclWI GGATC 8 cut(s) 380, 393, 515, 725, 863, 876, 1049, 1062
AcuI CTGAAG 1 cut(s) 852
AcvI CACGTG 2 cut(s) 875, 904
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 3 cut(s) 293, 564, 944
AfiI CCNNNNNNNGG 4 cut(s) 110, 416, 1004, 1106
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 4 cut(s) 430, 529, 742, 1132
AjiI CACGTC 1 cut(s) 804
AleI CACNNNNGTG 1 cut(s) 756
AluBI AGCT 4 cut(s) 284, 618, 703, 970
AluI AGCT 4 cut(s) 284, 618, 703, 970
Alw26I GTCTC 5 cut(s) 454, 607, 670, 729, 742
AlwI GGATC 8 cut(s) 380, 393, 515, 725, 863, 876, 1049, 1062
AlwNI CAGNNNCTG 1 cut(s) 59
Ama87I CYCGRG 4 cut(s) 240, 393, 704, 934
Aor13HI TCCGGA 1 cut(s) 691
AspLEI GCGC 7 cut(s) 35, 322, 490, 511, 612, 757, 769
AsuC2I CCSGG 3 cut(s) 264, 274, 434
AsuHPI GGTGA 7 cut(s) 79, 543, 709, 799, 923, 1019, 1074
AvaI CYCGRG 4 cut(s) 240, 393, 704, 934
BamHI GGATCC 3 cut(s) 385, 868, 1054
BauI CACGAG 1 cut(s) 820
BbrPI CACGTG 2 cut(s) 875, 904
BbsI GAAGAC 4 cut(s) 41, 124, 219, 822
BccI CCATC 2 cut(s) 1007, 1045
BceAI ACGGC 4 cut(s) 394, 628, 681, 1084
BcnI CCSGG 3 cut(s) 264, 274, 434
BcoDI GTCTC 5 cut(s) 454, 607, 670, 729, 742
BfaI CTAG 3 cut(s) 500, 993, 1071
BfuAI ACCTGC 1 cut(s) 98
BisI GCNGC 3 cut(s) 483, 486, 980
BlsI GCNGC 3 cut(s) 484, 487, 981
Bme1390I CCNGG 3 cut(s) 264, 274, 434
BmeT110I CYCGRG 4 cut(s) 240, 393, 704, 934
BmgBI CACGTC 1 cut(s) 804
BmiI GGNNCC 3 cut(s) 387, 870, 1056
BmrFI CCNGG 3 cut(s) 264, 274, 434
BmsI GCATC 1 cut(s) 675
BoxI GACNNNNGTC 2 cut(s) 599, 927
BpiI GAAGAC 4 cut(s) 41, 124, 219, 822
BplI GAGNNNNNCTC 2 cut(s) 451, 483
Bpu10I CCTNAGC 1 cut(s) 966
BpuEI CTTGAG 1 cut(s) 1115
BpuMI CCSGG 3 cut(s) 264, 274, 434
BsaAI YACGTR 2 cut(s) 875, 904
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 644, 935
BsaWI WCCGGW 1 cut(s) 691
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 416, 1004, 1106
Bse118I RCCGGY 1 cut(s) 1142
Bse1I ACTGG 2 cut(s) 53, 914
BseAI TCCGGA 1 cut(s) 691
BseDI CCNNGG 2 cut(s) 644, 935
BseGI GGATG 4 cut(s) 884, 945, 1018, 1056
BseLI CCNNNNNNNGG 4 cut(s) 110, 416, 1004, 1106
BseMII CTCAG 4 cut(s) 449, 740, 957, 1152
BseNI ACTGG 2 cut(s) 53, 914
BseRI GAGGAG 2 cut(s) 17, 722
BseYI CCCAGC 1 cut(s) 1105
Bsh1236I CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
BsiHKCI CYCGRG 4 cut(s) 240, 393, 704, 934
BsiSI CCGG 9 cut(s) 104, 179, 264, 274, 417, 433, 692, 950, 1143
BslFI GGGAC 2 cut(s) 140, 869
BslI CCNNNNNNNGG 4 cut(s) 110, 416, 1004, 1106
BsmAI GTCTC 5 cut(s) 454, 607, 670, 729, 742
BsmFI GGGAC 2 cut(s) 140, 869
Bso31I GGTCTC 1 cut(s) 729
BsoBI CYCGRG 4 cut(s) 240, 393, 704, 934
Bsp13I TCCGGA 1 cut(s) 691
Bsp143I GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
BspCNI CTCAG 4 cut(s) 450, 739, 958, 1151
BspEI TCCGGA 1 cut(s) 691
BspFNI CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
BspLI GGNNCC 3 cut(s) 387, 870, 1056
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 8 cut(s) 380, 393, 515, 725, 863, 876, 1049, 1062
BspTNI GGTCTC 1 cut(s) 729
BsrBI CCGCTC 2 cut(s) 120, 1048
BsrFI RCCGGY 1 cut(s) 1142
BsrI ACTGG 2 cut(s) 53, 914
BssAI RCCGGY 1 cut(s) 1142
BssECI CCNNGG 2 cut(s) 644, 935
BssMI GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
BssNI GRCGYC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 820
Bst2BI CACGAG 1 cut(s) 820
Bst4CI ACNGT 5 cut(s) 73, 916, 926, 1025, 1123
Bst6I CTCTTC 3 cut(s) 169, 228, 315
BstACI GRCGYC 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 875, 904
BstC8I GCNNGC 2 cut(s) 809, 1089
BstDEI CTNAG 6 cut(s) 458, 726, 966, 983, 1038, 1138
BstDSI CCRYGG 1 cut(s) 644
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 4 cut(s) 884, 945, 1018, 1056
BstFNI CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
BstHHI GCGC 7 cut(s) 35, 322, 490, 511, 612, 757, 769
BstKTI GATC 6 cut(s) 388, 523, 720, 775, 871, 1057
BstMAI GTCTC 5 cut(s) 454, 607, 670, 729, 742
BstMBI GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
BstMWI GCNNNNNNNGC 2 cut(s) 775, 976
BstNSI RCATGY 1 cut(s) 538
BstPAI GACNNNNGTC 2 cut(s) 599, 927
BstPI GGTNACC 1 cut(s) 929
BstSCI CCNGG 3 cut(s) 262, 272, 432
BstUI CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
BstV2I GAAGAC 4 cut(s) 41, 124, 219, 822
BstX2I RGATCY 3 cut(s) 385, 868, 1054
BstYI RGATCY 3 cut(s) 385, 868, 1054
BtgI CCRYGG 1 cut(s) 644
BtgZI GCGATG 1 cut(s) 698
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 4 cut(s) 884, 945, 1018, 1056
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 2 cut(s) 809, 1089
CaiI CAGNNNCTG 1 cut(s) 59
CfoI GCGC 7 cut(s) 35, 322, 490, 511, 612, 757, 769
Cfr10I RCCGGY 1 cut(s) 1142
Csp6I GTAC 3 cut(s) 292, 563, 943
CviAII CATG 2 cut(s) 535, 558
CviQI GTAC 3 cut(s) 292, 563, 943
DdeI CTNAG 6 cut(s) 458, 726, 966, 983, 1038, 1138
DpnI GATC 6 cut(s) 387, 522, 719, 774, 870, 1056
DpnII GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
Eam1104I CTCTTC 3 cut(s) 169, 228, 315
EarI CTCTTC 3 cut(s) 169, 228, 315
Eco31I GGTCTC 1 cut(s) 729
Eco32I GATATC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 852
Eco72I CACGTG 2 cut(s) 875, 904
Eco88I CYCGRG 4 cut(s) 240, 393, 704, 934
Eco91I GGTNACC 1 cut(s) 929
EcoO65I GGTNACC 1 cut(s) 929
EcoRV GATATC 1 cut(s) 6
FaeI CATG 2 cut(s) 538, 561
FaqI GGGAC 2 cut(s) 140, 869
FatI CATG 2 cut(s) 534, 557
FauI CCCGC 4 cut(s) 414, 804, 858, 895
FblI GTMKAC 2 cut(s) 372, 493
Fnu4HI GCNGC 3 cut(s) 483, 486, 980
FokI GGATG 4 cut(s) 891, 952, 1025, 1063
Fsp4HI GCNGC 3 cut(s) 483, 486, 980
FspBI CTAG 3 cut(s) 500, 993, 1071
GlaI GCGC 7 cut(s) 34, 321, 489, 510, 611, 756, 768
GluI GCNGC 3 cut(s) 483, 486, 980
GsaI CCCAGC 1 cut(s) 1109
HapII CCGG 9 cut(s) 104, 179, 264, 274, 417, 433, 692, 950, 1143
HhaI GCGC 7 cut(s) 35, 322, 490, 511, 612, 757, 769
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 2 cut(s) 538, 561
Hin6I GCGC 7 cut(s) 33, 320, 488, 509, 610, 755, 767
HinP1I GCGC 7 cut(s) 33, 320, 488, 509, 610, 755, 767
HincII GTYRAC 2 cut(s) 247, 919
HindII GTYRAC 2 cut(s) 247, 919
HinfI GANTC 9 cut(s) 161, 190, 202, 253, 348, 358, 818, 1017, 1136
HpaI GTTAAC 2 cut(s) 247, 919
HpaII CCGG 9 cut(s) 104, 179, 264, 274, 417, 433, 692, 950, 1143
HphI GGTGA 7 cut(s) 79, 543, 709, 799, 923, 1019, 1074
Hpy166II GTNNAC 7 cut(s) 247, 373, 379, 494, 663, 907, 919
Hpy188I TCNGA 5 cut(s) 12, 189, 390, 717, 1030
Hpy188III TCNNGA 7 cut(s) 147, 427, 515, 625, 692, 830, 1094
Hpy8I GTNNAC 7 cut(s) 247, 373, 379, 494, 663, 907, 919
Hpy99I CGWCG 7 cut(s) 16, 304, 307, 347, 521, 610, 926
HpyAV CCTTC 2 cut(s) 564, 863
HpyCH4III ACNGT 5 cut(s) 73, 916, 926, 1025, 1123
HpyCH4IV ACGT 6 cut(s) 14, 302, 375, 803, 874, 903
HpyF10VI GCNNNNNNNGC 2 cut(s) 775, 976
HpyF3I CTNAG 6 cut(s) 458, 726, 966, 983, 1038, 1138
HpySE526I ACGT 6 cut(s) 14, 302, 375, 803, 874, 903
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 2 cut(s) 538, 561
HspAI GCGC 7 cut(s) 33, 320, 488, 509, 610, 755, 767
Kpn2I TCCGGA 1 cut(s) 691
KspAI GTTAAC 2 cut(s) 247, 919
Kzo9I GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
LmnI GCTCC 1 cut(s) 578
LweI GCATC 1 cut(s) 675
MaeI CTAG 3 cut(s) 500, 993, 1071
MaeII ACGT 6 cut(s) 14, 302, 375, 803, 874, 903
MaeIII GTNAC 2 cut(s) 839, 929
MalI GATC 6 cut(s) 387, 522, 719, 774, 870, 1056
MbiI CCGCTC 2 cut(s) 120, 1048
MboI GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
MflI RGATCY 3 cut(s) 385, 868, 1054
MluCI AATT 2 cut(s) 529, 567
MlyI GAGTC 3 cut(s) 155, 812, 1130
MmeI TCCRAC 2 cut(s) 35, 413
MroI TCCGGA 1 cut(s) 691
MseI TTAA 6 cut(s) 246, 296, 633, 795, 858, 918
MslI CAYNNNNRTG 3 cut(s) 556, 756, 877
MspI CCGG 9 cut(s) 104, 179, 264, 274, 417, 433, 692, 950, 1143
MspR9I CCNGG 3 cut(s) 264, 274, 434
MvnI CGCG 9 cut(s) 320, 383, 490, 509, 610, 683, 755, 757, 769
MwoI GCNNNNNNNGC 2 cut(s) 775, 976
NciI CCSGG 3 cut(s) 264, 274, 434
NdeII GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
NlaIII CATG 2 cut(s) 538, 561
NlaIV GGNNCC 3 cut(s) 387, 870, 1056
NmuCI GTSAC 1 cut(s) 929
NspI RCATGY 1 cut(s) 538
OliI CACNNNNGTG 1 cut(s) 756
PaeR7I CTCGAG 1 cut(s) 704
PaqCI CACCTGC 1 cut(s) 98
PciI ACATGT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 2 cut(s) 311, 513
PfeI GAWTC 6 cut(s) 190, 202, 253, 348, 358, 1017
PflMI CCANNNNNTGG 1 cut(s) 1004
PkrI GCNGC 3 cut(s) 484, 487, 981
PleI GAGTC 3 cut(s) 155, 812, 1130
PmaCI CACGTG 2 cut(s) 875, 904
PmlI CACGTG 2 cut(s) 875, 904
PpsI GAGTC 3 cut(s) 155, 812, 1130
Ppu21I YACGTR 2 cut(s) 875, 904
PscI ACATGT 1 cut(s) 534
PshAI GACNNNNGTC 2 cut(s) 599, 927
PspCI CACGTG 2 cut(s) 875, 904
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 1105
PspN4I GGNNCC 3 cut(s) 387, 870, 1056
PspXI VCTCGAGB 1 cut(s) 704
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 3 cut(s) 385, 868, 1054
RsaI GTAC 3 cut(s) 293, 564, 944
RsaNI GTAC 3 cut(s) 292, 563, 943
RseI CAYNNNNRTG 3 cut(s) 556, 756, 877
SaqAI TTAA 6 cut(s) 246, 296, 633, 795, 858, 918
SatI GCNGC 3 cut(s) 483, 486, 980
Sau3AI GATC 6 cut(s) 385, 520, 717, 772, 868, 1054
SchI GAGTC 3 cut(s) 155, 812, 1130
ScrFI CCNGG 3 cut(s) 264, 274, 434
SfaNI GCATC 1 cut(s) 675
Sfr274I CTCGAG 1 cut(s) 704
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 3 cut(s) 556, 756, 877
SmlI CTYRAG 2 cut(s) 704, 1094
SmoI CTYRAG 2 cut(s) 704, 1094
Sse9I AATT 2 cut(s) 529, 567
SspMI CTAG 3 cut(s) 500, 993, 1071
StyD4I CCNGG 3 cut(s) 262, 272, 432
TaaI ACNGT 5 cut(s) 73, 916, 926, 1025, 1123
TaiI ACGT 6 cut(s) 17, 305, 378, 806, 877, 906
TasI AATT 2 cut(s) 529, 567
TatI WGTACW 1 cut(s) 291
TauI GCSGC 3 cut(s) 485, 488, 982
TfiI GAWTC 6 cut(s) 190, 202, 253, 348, 358, 1017
Tru1I TTAA 6 cut(s) 246, 296, 633, 795, 858, 918
Tru9I TTAA 6 cut(s) 246, 296, 633, 795, 858, 918
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 1 cut(s) 929
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 2 cut(s) 546, 610
TspGWI ACGGA 5 cut(s) 118, 303, 360, 760, 1047
TspRI CASTG 1 cut(s) 60
Van91I CCANNNNNTGG 1 cut(s) 1004
XceI RCATGY 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 704
XmiI GTMKAC 2 cut(s) 372, 493
XspI CTAG 3 cut(s) 500, 993, 1071
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.