Prupe.7G026200_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015487
Physical Location & Seq
Reverse (-)
4270850 .. 4271977
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G026200.1

Sequence Viewer

Length: 1128 bp
ATGCTCTCCCCAACGCCATTTTCTCTCATTTGCGCCGTTCTTCTCTGTTTGCCTCTCACCATAATCTTCACCATCACAAGCCCAACCACCACCATAACCACCACCATCCCCACCCAAATCTCCCAATCCCTAAAACTCACCAAAACCTACCAAAAAATCAACCTAATTTCACTGCCAAAATCCCTCCCATTAGATGACGACTCGCTCTTCCACCTCGCCGCCCGTGTTAAGTCCCGCCCGCCTCGCTCCGATCATCCCAAAATCGCCTTCCTCTTCCTAACCACCACCCCTCTTCCCTTCTCACCTCTCTGGGAATGCTTCTTCAACCAAACCCCCAAAACCCATTTCTCTATATATGTCCACGCTGACCCGAGATTCCCCTACGACCCGCCGTTTTCTGGCATCTTCGCCCACCGGGTCATCCCCTCCCAACCCGCCCAGCGATTCACCTCCACTCTCATCTCCGCGACTCGTCGTTTGCTCGCCCACGCCCTCCTTGATGACCCCAAGAATGCCATGTTCGCGCTTCTCTCTCCTTCTTGTATCCCCATCCGGTCCTTCAACTTCACGTACCAAACCCTCGCTCGATCGAAGAAGAGCTTCATCGAGATTCTAGACAACGAGATCGGGGCGTACGACAGGTGGGCGGCGCGTGGGGAGGACGCAATGTTGCCACAGGTGAAGCTGGAGGAGTTTCGGATCGGGTCCCAATTCTGGATCCTGAAGCGGAAGCATGCGAGGGTGGTTGTGGGTGACCACCGGCTCTGGTCCAAGTTCAAGCTACCGTGCCAGCGTTGGGACACGTGTTACCCGGAGGAAAATTACTTTCCTACCCTCCTGAACATGAGAGAACCAGGCGGGTGCGTGCCCGCTACCCTCACGCATGTGGATTGGCGCGGGAGATTCGATGGCCACCCCCGAACGTATAAGGCCTCCGACTTGGGGCCCCATTTGATCACCACCTTGAGAAATGATAGGCCAAAATACGGTGACGAGAAGGAGAGCGGCAACGGTTCTGCTTGGTCCTTGACAGAACGGCGAGACCCATTTCTATTCGCAAGGAAGTTCCCGCCGGATGCGATTGGACCGTTGATGAGTATGGCCAATGACGTCATCTTCAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

376

Amino Acids

42.78

Weight (kDa)

9.41

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1113
AccBSI CCGCTC 1 cut(s) 1005
AccII CGCG 4 cut(s) 467, 524, 652, 897
AclWI GGATC 3 cut(s) 707, 712, 725
AcoI YGGCCR 2 cut(s) 910, 1101
AcuI CTGAAG 1 cut(s) 743
AcvI CACGTG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 1110
AfaI GTAC 2 cut(s) 572, 635
AfiI CCNNNNNNNGG 5 cut(s) 398, 714, 796, 942, 986
AflIII ACRYGT 2 cut(s) 801, 803
AgsI TTSAA 4 cut(s) 325, 562, 778, 1120
AjnI CCWGG 1 cut(s) 853
AjuI GAANNNNNNNTTGG 5 cut(s) 36, 251, 283, 329, 361
AleI CACNNNNGTG 1 cut(s) 884
AluBI AGCT 3 cut(s) 600, 685, 781
AluI AGCT 3 cut(s) 600, 685, 781
Alw26I GTCTC 1 cut(s) 1035
AlwI GGATC 3 cut(s) 707, 712, 725
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 5 cut(s) 910, 930, 944, 977, 1101
ApaI GGGCCC 1 cut(s) 948
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
Asp700I GAANNNNTTC 1 cut(s) 599
AspLEI GCGC 4 cut(s) 35, 526, 652, 897
AspS9I GGNCC 7 cut(s) 555, 705, 768, 944, 945, 1023, 1085
AsuC2I CCSGG 2 cut(s) 416, 812
AsuHPI GGTGA 9 cut(s) 49, 61, 130, 294, 439, 691, 764, 949, 1001
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 5 cut(s) 555, 705, 768, 1023, 1085
BaeGI GKGCMC 2 cut(s) 870, 948
BalI TGGCCA 2 cut(s) 912, 1103
BamHI GGATCC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 948
BbrPI CACGTG 1 cut(s) 804
BccI CCATC 4 cut(s) 80, 113, 557, 902
BceAI ACGGC 3 cut(s) 20, 376, 1052
BciT130I CCWGG 1 cut(s) 855
BciVI GTATCC 1 cut(s) 554
BclI TGATCA 1 cut(s) 954
BcnI CCSGG 2 cut(s) 416, 812
BcoDI GTCTC 1 cut(s) 1035
BfaI CTAG 1 cut(s) 614
BfuI GTATCC 1 cut(s) 554
BisI GCNGC 3 cut(s) 219, 648, 1006
BlsI GCNGC 3 cut(s) 220, 649, 1007
Bme1390I CCNGG 3 cut(s) 416, 812, 855
Bme18I GGWCC 5 cut(s) 555, 705, 768, 1023, 1085
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 7 cut(s) 555, 705, 768, 944, 945, 1023, 1085
BmiI GGNNCC 6 cut(s) 706, 707, 719, 945, 946, 947
BmrFI CCNGG 3 cut(s) 416, 812, 855
BmsI GCATC 2 cut(s) 411, 1066
BpmI CTGGAG 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 985
BpuMI CCSGG 2 cut(s) 416, 812
BsaAI YACGTR 2 cut(s) 570, 804
BsaHI GRCGYC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 1035
BsaWI WCCGGW 1 cut(s) 552
Bsc4I CCNNNNNNNGG 5 cut(s) 398, 714, 796, 942, 986
Bse118I RCCGGY 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 672
BseBI CCWGG 1 cut(s) 855
BseGI GGATG 5 cut(s) 105, 253, 420, 549, 1081
BseLI CCNNNNNNNGG 5 cut(s) 398, 714, 796, 942, 986
BseMI GCAATG 1 cut(s) 672
BseRI GAGGAG 1 cut(s) 704
BseSI GKGCMC 2 cut(s) 870, 948
BseYI CCCAGC 1 cut(s) 438
Bsh1236I CGCG 4 cut(s) 467, 524, 652, 897
Bsh1285I CGRYCG 1 cut(s) 590
BshFI GGCC 5 cut(s) 912, 932, 946, 979, 1103
BsiEI CGRYCG 1 cut(s) 590
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 5 cut(s) 415, 553, 760, 812, 1073
BsiWI CGTACG 1 cut(s) 633
BslFI GGGAC 3 cut(s) 217, 691, 812
BslI CCNNNNNNNGG 5 cut(s) 398, 714, 796, 942, 986
BsmAI GTCTC 1 cut(s) 1035
BsmFI GGGAC 3 cut(s) 217, 691, 812
BsmI GAATGC 2 cut(s) 320, 517
BsnI GGCC 5 cut(s) 912, 932, 946, 979, 1103
Bso31I GGTCTC 1 cut(s) 1035
BsoBI CYCGRG 1 cut(s) 370
Bsp120I GGGCCC 1 cut(s) 944
Bsp1286I GDGCHC 2 cut(s) 870, 948
Bsp143I GATC 6 cut(s) 250, 587, 624, 699, 717, 954
BspANI GGCC 5 cut(s) 912, 932, 946, 979, 1103
BspFNI CGCG 4 cut(s) 467, 524, 652, 897
BspLI GGNNCC 6 cut(s) 706, 707, 719, 945, 946, 947
BspPI GGATC 3 cut(s) 707, 712, 725
BspQI GCTCTTC 2 cut(s) 212, 590
BspTNI GGTCTC 1 cut(s) 1035
BsrBI CCGCTC 1 cut(s) 1005
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 759
BssAI RCCGGY 1 cut(s) 759
BssMI GATC 6 cut(s) 250, 587, 624, 699, 717, 954
BssNI GRCGYC 1 cut(s) 1110
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 4 cut(s) 786, 989, 1013, 1089
Bst6I CTCTTC 4 cut(s) 212, 278, 297, 590
BstACI GRCGYC 1 cut(s) 1110
BstBAI YACGTR 2 cut(s) 570, 804
BstC8I GCNNGC 6 cut(s) 239, 483, 735, 791, 866, 870
BstEII GGTNACC 1 cut(s) 752
BstF5I GGATG 5 cut(s) 105, 253, 420, 549, 1081
BstFNI CGCG 4 cut(s) 467, 524, 652, 897
BstHHI GCGC 4 cut(s) 35, 526, 652, 897
BstKTI GATC 6 cut(s) 253, 590, 627, 702, 720, 957
BstMAI GTCTC 1 cut(s) 1035
BstMBI GATC 6 cut(s) 250, 587, 624, 699, 717, 954
BstMCI CGRYCG 1 cut(s) 590
BstMWI GCNNNNNNNGC 2 cut(s) 243, 521
BstNI CCWGG 1 cut(s) 855
BstNSI RCATGY 2 cut(s) 737, 887
BstPI GGTNACC 1 cut(s) 752
BstSCI CCNGG 3 cut(s) 414, 810, 853
BstSLI GKGCMC 2 cut(s) 870, 948
BstUI CGCG 4 cut(s) 467, 524, 652, 897
BstX2I RGATCY 1 cut(s) 717
BstYI RGATCY 1 cut(s) 717
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 5 cut(s) 912, 932, 946, 979, 1103
BtsCI GGATG 5 cut(s) 105, 253, 420, 549, 1081
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 6 cut(s) 239, 483, 735, 791, 866, 870
CfoI GCGC 4 cut(s) 35, 526, 652, 897
Cfr10I RCCGGY 1 cut(s) 759
Cfr13I GGNCC 7 cut(s) 555, 705, 768, 944, 945, 1023, 1085
CseI GACGC 1 cut(s) 671
Csp6I GTAC 2 cut(s) 571, 634
CviAII CATG 4 cut(s) 517, 734, 844, 884
CviQI GTAC 2 cut(s) 571, 634
DpnI GATC 6 cut(s) 252, 589, 626, 701, 719, 956
DpnII GATC 6 cut(s) 250, 587, 624, 699, 717, 954
EaeI YGGCCR 2 cut(s) 910, 1101
Eam1104I CTCTTC 4 cut(s) 212, 278, 297, 590
EarI CTCTTC 4 cut(s) 212, 278, 297, 590
Eco147I AGGCCT 1 cut(s) 932
Eco24I GRGCYC 1 cut(s) 948
Eco31I GGTCTC 1 cut(s) 1035
Eco47I GGWCC 5 cut(s) 555, 705, 768, 1023, 1085
Eco57I CTGAAG 1 cut(s) 743
Eco72I CACGTG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 370
Eco91I GGTNACC 1 cut(s) 752
EcoO109I RGGNCCY 3 cut(s) 705, 944, 945
EcoO65I GGTNACC 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 948
FaeI CATG 4 cut(s) 520, 737, 847, 887
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 3 cut(s) 217, 691, 812
FatI CATG 4 cut(s) 516, 733, 843, 883
FauI CCCGC 8 cut(s) 242, 246, 396, 442, 851, 877, 890, 1077
FbaI TGATCA 1 cut(s) 954
Fnu4HI GCNGC 3 cut(s) 219, 648, 1006
FokI GGATG 5 cut(s) 92, 240, 407, 536, 1088
FriOI GRGCYC 1 cut(s) 948
Fsp4HI GCNGC 3 cut(s) 219, 648, 1006
FspBI CTAG 1 cut(s) 614
GlaI GCGC 4 cut(s) 34, 525, 651, 896
GluI GCNGC 3 cut(s) 219, 648, 1006
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 1 cut(s) 707
HaeIII GGCC 5 cut(s) 912, 932, 946, 979, 1103
HapII CCGG 5 cut(s) 415, 553, 760, 812, 1073
HgaI GACGC 1 cut(s) 671
HhaI GCGC 4 cut(s) 35, 526, 652, 897
Hin1I GRCGYC 1 cut(s) 1110
Hin1II CATG 4 cut(s) 520, 737, 847, 887
Hin6I GCGC 4 cut(s) 33, 524, 650, 895
HinP1I GCGC 4 cut(s) 33, 524, 650, 895
HinfI GANTC 6 cut(s) 200, 375, 444, 469, 610, 903
HpaII CCGG 5 cut(s) 415, 553, 760, 812, 1073
HphI GGTGA 9 cut(s) 49, 61, 130, 294, 439, 691, 764, 949, 1001
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 250, 699, 937
Hpy188III TCNNGA 5 cut(s) 607, 614, 715, 721, 838
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 1 cut(s) 477
HpyAV CCTTC 5 cut(s) 277, 307, 546, 568, 991
HpyCH4III ACNGT 4 cut(s) 786, 989, 1013, 1089
HpyCH4IV ACGT 4 cut(s) 569, 803, 923, 1110
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 521
HpySE526I ACGT 4 cut(s) 569, 803, 923, 1110
Hsp92I GRCGYC 1 cut(s) 1110
Hsp92II CATG 4 cut(s) 520, 737, 847, 887
HspAI GCGC 4 cut(s) 33, 524, 650, 895
KflI GGGWCCC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 954
Kzo9I GATC 6 cut(s) 250, 587, 624, 699, 717, 954
LguI GCTCTTC 2 cut(s) 212, 590
LmnI GCTCC 1 cut(s) 251
LweI GCATC 2 cut(s) 411, 1066
MaeI CTAG 1 cut(s) 614
MaeII ACGT 4 cut(s) 569, 803, 923, 1110
MaeIII GTNAC 3 cut(s) 752, 806, 989
MalI GATC 6 cut(s) 252, 589, 626, 701, 719, 956
MbiI CCGCTC 1 cut(s) 1005
MboI GATC 6 cut(s) 250, 587, 624, 699, 717, 954
MflI RGATCY 1 cut(s) 717
MhlI GDGCHC 2 cut(s) 870, 948
MlsI TGGCCA 2 cut(s) 912, 1103
MluCI AATT 3 cut(s) 165, 710, 820
MluNI TGGCCA 2 cut(s) 912, 1103
MlyI GAGTC 2 cut(s) 194, 463
MmeI TCCRAC 1 cut(s) 960
Mox20I TGGCCA 2 cut(s) 912, 1103
MroXI GAANNNNTTC 1 cut(s) 599
MscI TGGCCA 2 cut(s) 912, 1103
MseI TTAA 2 cut(s) 228, 1126
MslI CAYNNNNRTG 1 cut(s) 884
Msp20I TGGCCA 2 cut(s) 912, 1103
MspI CCGG 5 cut(s) 415, 553, 760, 812, 1073
MspR9I CCNGG 3 cut(s) 416, 812, 855
Mva1269I GAATGC 2 cut(s) 320, 517
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 4 cut(s) 467, 524, 652, 897
MwoI GCNNNNNNNGC 2 cut(s) 243, 521
NciI CCSGG 2 cut(s) 416, 812
NdeII GATC 6 cut(s) 250, 587, 624, 699, 717, 954
NlaIII CATG 4 cut(s) 520, 737, 847, 887
NlaIV GGNNCC 6 cut(s) 706, 707, 719, 945, 946, 947
NmuCI GTSAC 2 cut(s) 752, 989
NspI RCATGY 2 cut(s) 737, 887
OliI CACNNNNGTG 1 cut(s) 884
PaeI GCATGC 1 cut(s) 737
PceI AGGCCT 1 cut(s) 932
PciSI GCTCTTC 2 cut(s) 212, 590
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 2 cut(s) 320, 517
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 4 cut(s) 375, 444, 610, 903
Pfl23II CGTACG 1 cut(s) 633
PkrI GCNGC 3 cut(s) 220, 649, 1007
Ple19I CGATCG 1 cut(s) 590
PleI GAGTC 2 cut(s) 194, 463
PmaCI CACGTG 1 cut(s) 804
PmlI CACGTG 1 cut(s) 804
PpsI GAGTC 2 cut(s) 194, 463
Ppu21I YACGTR 2 cut(s) 570, 804
PpuMI RGGWCCY 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 853
PspCI CACGTG 1 cut(s) 804
PspEI GGTNACC 1 cut(s) 752
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 853
PspLI CGTACG 1 cut(s) 633
PspN4I GGNNCC 6 cut(s) 706, 707, 719, 945, 946, 947
PspOMI GGGCCC 1 cut(s) 944
PspPI GGNCC 7 cut(s) 555, 705, 768, 944, 945, 1023, 1085
PspPPI RGGWCCY 1 cut(s) 705
PsuI RGATCY 1 cut(s) 717
PvuI CGATCG 1 cut(s) 590
RsaI GTAC 2 cut(s) 572, 635
RsaNI GTAC 2 cut(s) 571, 634
RseI CAYNNNNRTG 1 cut(s) 884
SapI GCTCTTC 2 cut(s) 212, 590
SaqAI TTAA 2 cut(s) 228, 1126
SatI GCNGC 3 cut(s) 219, 648, 1006
Sau3AI GATC 6 cut(s) 250, 587, 624, 699, 717, 954
Sau96I GGNCC 7 cut(s) 555, 705, 768, 944, 945, 1023, 1085
SchI GAGTC 2 cut(s) 194, 463
ScrFI CCNGG 3 cut(s) 416, 812, 855
SduI GDGCHC 2 cut(s) 870, 948
SfaNI GCATC 2 cut(s) 411, 1066
SinI GGWCC 5 cut(s) 555, 705, 768, 1023, 1085
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 1 cut(s) 964
SmoI CTYRAG 1 cut(s) 964
SphI GCATGC 1 cut(s) 737
Sse9I AATT 3 cut(s) 165, 710, 820
SseBI AGGCCT 1 cut(s) 932
SspMI CTAG 1 cut(s) 614
StuI AGGCCT 1 cut(s) 932
StyD4I CCNGG 3 cut(s) 414, 810, 853
TaaI ACNGT 4 cut(s) 786, 989, 1013, 1089
TaiI ACGT 4 cut(s) 572, 806, 926, 1113
TaqI TCGA 4 cut(s) 586, 590, 606, 906
TasI AATT 3 cut(s) 165, 710, 820
TauI GCSGC 3 cut(s) 221, 650, 1008
TfiI GAWTC 4 cut(s) 375, 444, 610, 903
Tru1I TTAA 2 cut(s) 228, 1126
Tru9I TTAA 2 cut(s) 228, 1126
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 2 cut(s) 752, 989
Tsp45I GTSAC 2 cut(s) 752, 989
TspDTI ATGAA 1 cut(s) 592
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 5 cut(s) 555, 705, 768, 1023, 1085
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 2 cut(s) 737, 887
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 1 cut(s) 614
ZraI GACGTC 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.