Rmu_co8427971.1_g000001

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8427971.1
OG0015487
Physical Location & Seq
Forward (+)
31 .. 1092
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8427971.1_g000001.1.cds

Sequence Viewer

Length: 1062 bp
atgcccctcgccgtcgtcttcaccgtcaccagccccaccaacattcctctccaaatccccaaatcctcaacgaaagcccaccggaaactaaacctgacgccccttcctccgcctgctccgctgcctttagatgacgcatctcttttacgaatcgccgcccgggttaactcgaggccgcccaggttcaagaagccgaaaatcgccttcatgttcctcaccaccaccccgctgccgttcgctcctctgtgggagcgattcttcagtaaggcccccaagtcgctgtacagaatctacgtccacgccgaccccagattcccttacgagcctcctttctccggcgtcttcgccggccgggttattcattcgaaacccgcccagcggttcacccccactctcatttccgccacgcgccgcctactcgcccacgcgctgctcgacgatccccggaactcgatgttcgcgcttctcactccctcgtgcatccccctccactccttcaacttcacgtaccggacgctggccgcgtcgaagaagagcttcatcgaaatcctcgacaacgagatcggggcgtacgacaggtgggcggcgcgtgggccggacgctatgctcccgcaggtgagcctggaggagttccggatcgggtcgcagttttgggtcctgaagcggaagcacgctcggcttgtggtgagtgaccagcggctttggtccaagttcaagctcccgtgcgagcggtgggacacgtgttaccctgaggaaaattacttccctaccctcgtcaacatgcgcgaccctgacgggtgcgtgcctgccacgctcacgcacgtggactggaccgggagggtcgacggccacccggtgacgtacaacgcctccgacgtggggccggacctgatctggtcgctgagggaggacaggcccaggtacggcgacgaggtgaacgtcaacgccaccgattggtccggcagcgaacgccacgatccgtttcttttcgcgaggaagttcccgcctgacgcaatcgagccgttgatgagtatggcgagtgacgtcattttcagagactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

353

Amino Acids

40.24

Weight (kDa)

9.4

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
AatII GACGTC 1 cut(s) 1047
Acc36I ACCTGC 1 cut(s) 604
AccB7I CCANNNNNTGG 1 cut(s) 954
AccBSI CCGCTC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 843
AccII CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
AccIII TCCGGA 1 cut(s) 633
AclWI GGATC 3 cut(s) 434, 644, 971
AcoI YGGCCR 3 cut(s) 349, 519, 847
AcuI CTGAAG 2 cut(s) 244, 680
AcvI CACGTG 2 cut(s) 741, 823
AcyI GRCGYC 3 cut(s) 98, 339, 1044
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 5 cut(s) 284, 509, 572, 863, 923
AfiI CCNNNNNNNGG 6 cut(s) 335, 378, 517, 879, 923, 954
AflIII ACRYGT 2 cut(s) 738, 740
AgsI TTSAA 3 cut(s) 187, 499, 715
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 3 cut(s) 179, 621, 917
AleI CACNNNNGTG 1 cut(s) 821
AluBI AGCT 2 cut(s) 537, 718
AluI AGCT 2 cut(s) 537, 718
Alw26I GTCTC 1 cut(s) 1050
AlwI GGATC 3 cut(s) 434, 644, 971
Ama87I CYCGRG 2 cut(s) 159, 169
Aor13HI TCCGGA 1 cut(s) 633
AoxI GGCC 8 cut(s) 173, 267, 349, 519, 593, 847, 881, 914
ApeKI GCWGC 4 cut(s) 121, 229, 430, 963
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 5 cut(s) 411, 430, 463, 589, 786
AspS9I GGNCC 9 cut(s) 268, 593, 655, 705, 831, 881, 886, 915, 957
AsuC2I CCSGG 6 cut(s) 160, 161, 353, 445, 835, 854
AsuHPI GGTGA 8 cut(s) 13, 19, 208, 376, 628, 697, 868, 946
AsuII TTCGAA 1 cut(s) 365
AvaI CYCGRG 2 cut(s) 159, 169
AvaII GGWCC 5 cut(s) 655, 705, 831, 886, 957
AxyI CCTNAGG 1 cut(s) 750
BauI CACGAG 1 cut(s) 475
BbrPI CACGTG 2 cut(s) 741, 823
BbsI GAAGAC 2 cut(s) 10, 334
BbvCI CCTCAGC 1 cut(s) 902
BbvI GCAGC 4 cut(s) 108, 216, 417, 975
BceAI ACGGC 4 cut(s) 217, 862, 940, 1006
BciT130I CCWGG 3 cut(s) 181, 623, 919
BcnI CCSGG 6 cut(s) 160, 161, 353, 445, 835, 854
BcoDI GTCTC 1 cut(s) 1050
BfuAI ACCTGC 1 cut(s) 604
Bme1390I CCNGG 9 cut(s) 160, 161, 181, 353, 445, 623, 835, 854, 919
Bme18I GGWCC 5 cut(s) 655, 705, 831, 886, 957
BmeT110I CYCGRG 2 cut(s) 159, 169
BmgBI CACGTC 1 cut(s) 877
BmgT120I GGNCC 9 cut(s) 268, 593, 655, 705, 831, 881, 886, 915, 957
BmiI GGNNCC 3 cut(s) 270, 656, 882
BmrFI CCNGG 9 cut(s) 160, 161, 181, 353, 445, 623, 835, 854, 919
BmsI GCATC 2 cut(s) 146, 489
BpiI GAAGAC 2 cut(s) 10, 334
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 1 cut(s) 902
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 6 cut(s) 160, 161, 353, 445, 835, 854
BsaAI YACGTR 3 cut(s) 507, 741, 823
BsaHI GRCGYC 3 cut(s) 98, 339, 1044
BsaJI CCNNGG 4 cut(s) 159, 179, 443, 917
BsaWI WCCGGW 3 cut(s) 81, 510, 633
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 6 cut(s) 335, 378, 517, 879, 923, 954
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 833
Bse21I CCTNAGG 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 633
BseBI CCWGG 3 cut(s) 181, 623, 919
BseDI CCNNGG 4 cut(s) 159, 179, 443, 917
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 6 cut(s) 335, 378, 517, 879, 923, 954
BseMII CTCAG 2 cut(s) 741, 893
BseNI ACTGG 1 cut(s) 833
BseRI GAGGAG 2 cut(s) 231, 641
BseX3I CGGCCG 1 cut(s) 349
BseXI GCAGC 4 cut(s) 108, 216, 417, 975
BseYI CCCAGC 1 cut(s) 375
Bsh1236I CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
Bsh1285I CGRYCG 1 cut(s) 352
BshFI GGCC 8 cut(s) 175, 269, 351, 521, 595, 849, 883, 916
BsiEI CGRYCG 1 cut(s) 352
BsiHKCI CYCGRG 2 cut(s) 159, 169
BsiWI CGTACG 1 cut(s) 570
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 6 cut(s) 335, 378, 517, 879, 923, 954
BsmAI GTCTC 1 cut(s) 1050
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 8 cut(s) 175, 269, 351, 521, 595, 849, 883, 916
BsoBI CYCGRG 2 cut(s) 159, 169
Bsp119I TTCGAA 1 cut(s) 365
Bsp13I TCCGGA 1 cut(s) 633
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 5 cut(s) 439, 561, 636, 891, 976
Bsp68I TCGCGA 1 cut(s) 992
BspANI GGCC 8 cut(s) 175, 269, 351, 521, 595, 849, 883, 916
BspCNI CTCAG 2 cut(s) 742, 894
BspEI TCCGGA 1 cut(s) 633
BspFNI CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
BspLI GGNNCC 3 cut(s) 270, 656, 882
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 3 cut(s) 434, 644, 971
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 1 cut(s) 833
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 4 cut(s) 159, 179, 443, 917
BssMI GATC 5 cut(s) 439, 561, 636, 891, 976
BssNI GRCGYC 3 cut(s) 98, 339, 1044
BssSI CACGAG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 181, 623, 919
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 527
BstACI GRCGYC 3 cut(s) 98, 339, 1044
BstAUI TGTACA 1 cut(s) 282
BstBAI YACGTR 3 cut(s) 507, 741, 823
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 7 cut(s) 114, 349, 519, 672, 728, 803, 807
BstDEI CTNAG 2 cut(s) 750, 902
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
BstHHI GCGC 5 cut(s) 411, 430, 463, 589, 786
BstKTI GATC 5 cut(s) 442, 564, 639, 894, 979
BstMAI GTCTC 1 cut(s) 1050
BstMBI GATC 5 cut(s) 439, 561, 636, 891, 976
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 4 cut(s) 118, 676, 811, 969
BstNI CCWGG 3 cut(s) 181, 623, 919
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 9 cut(s) 158, 159, 179, 351, 443, 621, 833, 852, 917
BstUI CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
BstV1I GCAGC 4 cut(s) 108, 216, 417, 975
BstV2I GAAGAC 2 cut(s) 10, 334
BstZI CGGCCG 1 cut(s) 349
Bsu36I CCTNAGG 1 cut(s) 750
BsuRI GGCC 8 cut(s) 175, 269, 351, 521, 595, 849, 883, 916
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 1 cut(s) 480
BtuMI TCGCGA 1 cut(s) 992
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 7 cut(s) 114, 349, 519, 672, 728, 803, 807
CfoI GCGC 5 cut(s) 411, 430, 463, 589, 786
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 9 cut(s) 268, 593, 655, 705, 831, 881, 886, 915, 957
Cfr9I CCCGGG 1 cut(s) 159
CseI GACGC 7 cut(s) 106, 143, 328, 513, 523, 608, 1019
Csp6I GTAC 5 cut(s) 283, 508, 571, 862, 922
CviAII CATG 2 cut(s) 208, 781
CviQI GTAC 5 cut(s) 283, 508, 571, 862, 922
DdeI CTNAG 2 cut(s) 750, 902
DpnI GATC 5 cut(s) 441, 563, 638, 893, 978
DpnII GATC 5 cut(s) 439, 561, 636, 891, 976
DraIII CACNNNGTG 1 cut(s) 856
EaeI YGGCCR 3 cut(s) 349, 519, 847
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
EciI GGCGGA 2 cut(s) 99, 391
EclXI CGGCCG 1 cut(s) 349
Eco47I GGWCC 5 cut(s) 655, 705, 831, 886, 957
Eco52I CGGCCG 1 cut(s) 349
Eco57I CTGAAG 2 cut(s) 244, 680
Eco72I CACGTG 2 cut(s) 741, 823
Eco81I CCTNAGG 1 cut(s) 750
Eco88I CYCGRG 2 cut(s) 159, 169
EcoO109I RGGNCCY 2 cut(s) 268, 655
EcoRII CCWGG 3 cut(s) 179, 621, 917
FaeI CATG 2 cut(s) 211, 784
FaiI YATR 4 cut(s) 209, 605, 782, 1034
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 1 cut(s) 749
FatI CATG 2 cut(s) 207, 780
FauI CCCGC 4 cut(s) 234, 379, 618, 1011
FblI GTMKAC 1 cut(s) 843
FokI GGATG 1 cut(s) 467
GlaI GCGC 5 cut(s) 410, 429, 462, 588, 785
GsaI CCCAGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 8 cut(s) 175, 269, 351, 521, 595, 849, 883, 916
HgaI GACGC 7 cut(s) 106, 143, 328, 513, 523, 608, 1019
HhaI GCGC 5 cut(s) 411, 430, 463, 589, 786
Hin1I GRCGYC 3 cut(s) 98, 339, 1044
Hin1II CATG 2 cut(s) 211, 784
Hin6I GCGC 5 cut(s) 409, 428, 461, 587, 784
HinP1I GCGC 5 cut(s) 409, 428, 461, 587, 784
HincII GTYRAC 4 cut(s) 166, 778, 844, 943
HindII GTYRAC 4 cut(s) 166, 778, 844, 943
HinfI GANTC 4 cut(s) 150, 255, 288, 312
HpaI GTTAAC 1 cut(s) 166
HphI GGTGA 8 cut(s) 13, 19, 208, 376, 628, 697, 868, 946
Hpy166II GTNNAC 8 cut(s) 166, 298, 384, 778, 826, 844, 937, 943
Hpy188I TCNGA 2 cut(s) 874, 1055
Hpy188III TCNNGA 4 cut(s) 187, 634, 658, 991
Hpy8I GTNNAC 8 cut(s) 166, 298, 384, 778, 826, 844, 937, 943
Hpy99I CGWCG 6 cut(s) 17, 440, 529, 848, 878, 932
HpyAV CCTTC 3 cut(s) 113, 214, 505
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 8 cut(s) 294, 506, 740, 822, 860, 876, 939, 1044
HpyCH4V TGCA 1 cut(s) 480
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 676, 811, 969
HpyF3I CTNAG 2 cut(s) 750, 902
HpySE526I ACGT 8 cut(s) 294, 506, 740, 822, 860, 876, 939, 1044
Hsp92I GRCGYC 3 cut(s) 98, 339, 1044
Hsp92II CATG 2 cut(s) 211, 784
HspAI GCGC 5 cut(s) 409, 428, 461, 587, 784
Kpn2I TCCGGA 1 cut(s) 633
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 5 cut(s) 439, 561, 636, 891, 976
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 5 cut(s) 121, 244, 250, 612, 723
Lsp1109I GCAGC 4 cut(s) 108, 216, 417, 975
LweI GCATC 2 cut(s) 146, 489
MaeII ACGT 8 cut(s) 294, 506, 740, 822, 860, 876, 939, 1044
MaeIII GTNAC 5 cut(s) 25, 689, 743, 856, 1040
MalI GATC 5 cut(s) 441, 563, 638, 893, 978
MbiI CCGCTC 1 cut(s) 730
MboI GATC 5 cut(s) 439, 561, 636, 891, 976
MboII GAAGA 5 cut(s) 10, 250, 334, 541, 544
MluCI AATT 1 cut(s) 757
MmeI TCCRAC 1 cut(s) 897
MroI TCCGGA 1 cut(s) 633
MroNI GCCGGC 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 821
MspA1I CMGCKG 4 cut(s) 121, 229, 379, 697
MspR9I CCNGG 9 cut(s) 160, 161, 181, 353, 445, 623, 835, 854, 919
MvaI CCWGG 3 cut(s) 181, 623, 919
MvnI CGCG 7 cut(s) 409, 428, 461, 524, 589, 786, 992
MwoI GCNNNNNNNGC 4 cut(s) 118, 676, 811, 969
NaeI GCCGGC 1 cut(s) 349
NciI CCSGG 6 cut(s) 160, 161, 353, 445, 835, 854
NdeII GATC 5 cut(s) 439, 561, 636, 891, 976
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 2 cut(s) 211, 784
NlaIV GGNNCC 3 cut(s) 270, 656, 882
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 4 cut(s) 25, 689, 856, 1040
NruI TCGCGA 1 cut(s) 992
NspI RCATGY 1 cut(s) 784
NspV TTCGAA 1 cut(s) 365
OliI CACNNNNGTG 1 cut(s) 821
PaeR7I CTCGAG 1 cut(s) 169
PaqCI CACCTGC 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 8 cut(s) 21, 300, 432, 521, 549, 570, 873, 936
PdiI GCCGGC 1 cut(s) 349
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 4 cut(s) 150, 255, 288, 312
Pfl23II CGTACG 1 cut(s) 570
PflMI CCANNNNNTGG 1 cut(s) 954
PmaCI CACGTG 2 cut(s) 741, 823
PmlI CACGTG 2 cut(s) 741, 823
Ppu21I YACGTR 3 cut(s) 507, 741, 823
PpuMI RGGWCCY 1 cut(s) 655
Psp5II RGGWCCY 1 cut(s) 655
Psp6I CCWGG 3 cut(s) 179, 621, 917
PspCI CACGTG 2 cut(s) 741, 823
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 3 cut(s) 179, 621, 917
PspLI CGTACG 1 cut(s) 570
PspN4I GGNNCC 3 cut(s) 270, 656, 882
PspPI GGNCC 9 cut(s) 268, 593, 655, 705, 831, 881, 886, 915, 957
PspPPI RGGWCCY 1 cut(s) 655
PspXI VCTCGAGB 1 cut(s) 169
RruI TCGCGA 1 cut(s) 992
RsaI GTAC 5 cut(s) 284, 509, 572, 863, 923
RsaNI GTAC 5 cut(s) 283, 508, 571, 862, 922
RseI CAYNNNNRTG 1 cut(s) 821
SalI GTCGAC 1 cut(s) 842
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 1 cut(s) 165
Sau3AI GATC 5 cut(s) 439, 561, 636, 891, 976
Sau96I GGNCC 9 cut(s) 268, 593, 655, 705, 831, 881, 886, 915, 957
ScrFI CCNGG 9 cut(s) 160, 161, 181, 353, 445, 623, 835, 854, 919
SfaNI GCATC 2 cut(s) 146, 489
Sfr274I CTCGAG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 5 cut(s) 655, 705, 831, 886, 957
SlaI CTCGAG 1 cut(s) 169
SmaI CCCGGG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 1 cut(s) 757
StyD4I CCNGG 9 cut(s) 158, 159, 179, 351, 443, 621, 833, 852, 917
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 8 cut(s) 297, 509, 743, 825, 863, 879, 942, 1047
TaqI TCGA 9 cut(s) 170, 365, 435, 452, 527, 543, 552, 843, 1017
TasI AATT 1 cut(s) 757
TatI WGTACW 1 cut(s) 282
TauI GCSGC 6 cut(s) 158, 178, 414, 524, 587, 700
TfiI GAWTC 4 cut(s) 150, 255, 288, 312
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseFI GTSAC 4 cut(s) 25, 689, 856, 1040
TseI GCWGC 4 cut(s) 121, 229, 430, 963
Tsp45I GTSAC 4 cut(s) 25, 689, 856, 1040
TspDTI ATGAA 3 cut(s) 196, 350, 529
TspGWI ACGGA 1 cut(s) 969
TspMI CCCGGG 1 cut(s) 159
Van91I CCANNNNNTGG 1 cut(s) 954
VpaK11BI GGWCC 5 cut(s) 655, 705, 831, 886, 957
XceI RCATGY 1 cut(s) 784
XhoI CTCGAG 1 cut(s) 169
XmaI CCCGGG 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 843
XmnI GAANNNNTTC 1 cut(s) 536
ZraI GACGTC 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.