RchiOBHm_Chr6g0297881

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015487
Physical Location & Seq
Reverse (-)
59505353 .. 59506868
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26738

Sequence Viewer

Length: 1071 bp
ATGCTGATCTCGCCCACCCCACTCTCTCTCATCTGTGCCCTCATACTCTGCATGCCCCTCGCCGTCGTCTTCACCGTCACCAGCCCCACCAACATTCCTCTCCAAATCCCCAAATCCTCAACCAAAGCCCACCGGAAACTAAACCTGACGCCTCTTCCTCCGCCTGCTCCGCTGCCTTTAGATGACGTATCTCTTTTACGAATCGCCGCCCGGGTTAACTCGAGGCCGCCCAGGTTCAAGAAGCCGAAAATCGCCTTCATGTTCCTCACCACCACCCCGCTGCCCTTCGCTCCTCTAATCTACGTCCACGCCGACCCCAGATTCCCTTACGAGCCTCCTTTCTCCGGCGTCTTCGCCGGCCGGGTTATTCATTCGAAACCCGCCCAGCGGTTCACCCCCACTCTCATTTCCGCCACGCGCCGCCTACTCGCCCACGCGCTGCTCGACGATCCCCGGAACTCGATGTTCGCGCTGCTCACTCCCTCGTGCATCCCCCTCCACTCCTTCAACTTCACGTACCGGACCCTGGCCGCGTCGAAGAAGAGCTTCATCGAAATCCTCGACAACGAGATCGGGGCGTACGACAGGTGGGCGGCGCGTGGGCCGGACGCGATGCTCCCGCATGTGAGCCTGGAGGAGTTCCGGATCGGGTCGCAGTTTTGGGTCCTGAAGCGGAAGCACGCTCGGCTTGTGGTGAGTGACCAGCGGCTTTGGTCCAAGTTCAAGCTCCCGTGCGAGCGGTGGGACACGTGTTACCCTGAGGAAAATTACTTCCCTACCCTCCTCAACATGCGCGACCCAGACGGGTGCGTGCCTGCCACGCTCACGCACGTGGACTGGACCGGGAGGGTCGACGGCCACCCGGTGACGTACAACGCCTCCGACGTGGGCCCAGACCTGATCTGGTCGCTGAGGGAGGACAGGCCCAGGTACGGCGACGAGGTGAACGTCAACGCCACCGATTGGTCCGGCAGCGAACGCCACGATCCGTTTCTTTTCGCGAGGAAGTTCCCGCCGGACGCAATCGAGCCGTTGATGAGTATGGCGAGTGACGTCATTTTCAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

356

Amino Acids

40.28

Weight (kDa)

9.0

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 99 - 303 1.4e-54 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1056
AccB7I CCANNNNNTGG 1 cut(s) 963
AccBSI CCGCTC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 852
AccII CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
AccIII TCCGGA 1 cut(s) 642
AclWI GGATC 3 cut(s) 443, 653, 980
AcoI YGGCCR 3 cut(s) 358, 528, 856
AcuI CTGAAG 1 cut(s) 689
AcvI CACGTG 2 cut(s) 750, 832
AcyI GRCGYC 3 cut(s) 149, 348, 1053
AdeI CACNNNGTG 1 cut(s) 865
AfaI GTAC 4 cut(s) 518, 581, 872, 932
AfiI CCNNNNNNNGG 5 cut(s) 344, 387, 526, 932, 963
AflIII ACRYGT 2 cut(s) 747, 749
AgsI TTSAA 3 cut(s) 238, 508, 724
AjiI CACGTC 1 cut(s) 886
AjnI CCWGG 4 cut(s) 230, 525, 630, 926
AleI CACNNNNGTG 1 cut(s) 830
AluBI AGCT 2 cut(s) 546, 727
AluI AGCT 2 cut(s) 546, 727
Alw26I GTCTC 1 cut(s) 1059
AlwI GGATC 3 cut(s) 443, 653, 980
Ama87I CYCGRG 2 cut(s) 210, 220
Aor13HI TCCGGA 1 cut(s) 642
AoxI GGCC 7 cut(s) 224, 358, 528, 602, 856, 889, 923
ApaI GGGCCC 1 cut(s) 893
ApeKI GCWGC 5 cut(s) 172, 280, 439, 472, 972
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 5 cut(s) 420, 439, 472, 598, 795
AspS9I GGNCC 9 cut(s) 522, 602, 664, 714, 840, 889, 890, 924, 966
AsuC2I CCSGG 6 cut(s) 211, 212, 362, 454, 844, 863
AsuHPI GGTGA 7 cut(s) 64, 70, 259, 385, 706, 877, 955
AsuII TTCGAA 1 cut(s) 374
AvaI CYCGRG 2 cut(s) 210, 220
AvaII GGWCC 5 cut(s) 522, 664, 714, 840, 966
AxyI CCTNAGG 1 cut(s) 759
BaeGI GKGCMC 2 cut(s) 40, 893
BanII GRGCYC 1 cut(s) 893
BauI CACGAG 1 cut(s) 484
BbrPI CACGTG 2 cut(s) 750, 832
BbsI GAAGAC 2 cut(s) 61, 343
BbvCI CCTCAGC 1 cut(s) 911
BbvI GCAGC 5 cut(s) 159, 267, 426, 459, 984
BceAI ACGGC 4 cut(s) 47, 871, 949, 1015
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 4 cut(s) 232, 527, 632, 928
BcnI CCSGG 6 cut(s) 211, 212, 362, 454, 844, 863
BcoDI GTCTC 1 cut(s) 1059
Bme18I GGWCC 5 cut(s) 522, 664, 714, 840, 966
BmeT110I CYCGRG 2 cut(s) 210, 220
BmgBI CACGTC 1 cut(s) 886
BmgT120I GGNCC 9 cut(s) 522, 602, 664, 714, 840, 889, 890, 924, 966
BmiI GGNNCC 3 cut(s) 524, 665, 891
BmsI GCATC 2 cut(s) 498, 603
BpiI GAAGAC 2 cut(s) 61, 343
BpmI CTGGAG 1 cut(s) 653
Bpu10I CCTNAGC 1 cut(s) 911
Bpu14I TTCGAA 1 cut(s) 374
BpuMI CCSGG 6 cut(s) 211, 212, 362, 454, 844, 863
BsaAI YACGTR 3 cut(s) 516, 750, 832
BsaHI GRCGYC 3 cut(s) 149, 348, 1053
BsaJI CCNNGG 5 cut(s) 210, 230, 452, 525, 926
BsaWI WCCGGW 3 cut(s) 132, 519, 642
BsaXI ACNNNNNCTCC 2 cut(s) 863, 893
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 387, 526, 932, 963
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 842
Bse21I CCTNAGG 1 cut(s) 759
BseAI TCCGGA 1 cut(s) 642
BseBI CCWGG 4 cut(s) 232, 527, 632, 928
BseDI CCNNGG 5 cut(s) 210, 230, 452, 525, 926
BseGI GGATG 1 cut(s) 489
BseLI CCNNNNNNNGG 5 cut(s) 344, 387, 526, 932, 963
BseMII CTCAG 2 cut(s) 750, 902
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 3 cut(s) 282, 650, 773
BseSI GKGCMC 2 cut(s) 40, 893
BseX3I CGGCCG 1 cut(s) 358
BseXI GCAGC 5 cut(s) 159, 267, 426, 459, 984
BseYI CCCAGC 1 cut(s) 384
Bsh1236I CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 7 cut(s) 226, 360, 530, 604, 858, 891, 925
BsiEI CGRYCG 1 cut(s) 361
BsiHKCI CYCGRG 2 cut(s) 210, 220
BsiWI CGTACG 1 cut(s) 579
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 5 cut(s) 344, 387, 526, 932, 963
BsmAI GTCTC 1 cut(s) 1059
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 7 cut(s) 226, 360, 530, 604, 858, 891, 925
BsoBI CYCGRG 2 cut(s) 210, 220
Bsp119I TTCGAA 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 889
Bsp1286I GDGCHC 2 cut(s) 40, 893
Bsp13I TCCGGA 1 cut(s) 642
Bsp143I GATC 6 cut(s) 6, 448, 570, 645, 900, 985
Bsp68I TCGCGA 1 cut(s) 1001
BspANI GGCC 7 cut(s) 226, 360, 530, 604, 858, 891, 925
BspCNI CTCAG 2 cut(s) 751, 903
BspEI TCCGGA 1 cut(s) 642
BspFNI CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
BspLI GGNNCC 3 cut(s) 524, 665, 891
BspPI GGATC 3 cut(s) 443, 653, 980
BspQI GCTCTTC 1 cut(s) 536
BspT104I TTCGAA 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 739
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 1 cut(s) 842
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 5 cut(s) 210, 230, 452, 525, 926
BssMI GATC 6 cut(s) 6, 448, 570, 645, 900, 985
BssNI GRCGYC 3 cut(s) 149, 348, 1053
BssSI CACGAG 1 cut(s) 484
Bst2BI CACGAG 1 cut(s) 484
Bst2UI CCWGG 4 cut(s) 232, 527, 632, 928
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 2 cut(s) 159, 536
BstACI GRCGYC 3 cut(s) 149, 348, 1053
BstBAI YACGTR 3 cut(s) 516, 750, 832
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 7 cut(s) 53, 165, 358, 681, 737, 812, 816
BstDEI CTNAG 2 cut(s) 759, 911
BstF5I GGATG 1 cut(s) 489
BstFNI CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
BstHHI GCGC 5 cut(s) 420, 439, 472, 598, 795
BstKTI GATC 6 cut(s) 9, 451, 573, 648, 903, 988
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 6 cut(s) 6, 448, 570, 645, 900, 985
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 5 cut(s) 10, 169, 685, 820, 978
BstNI CCWGG 4 cut(s) 232, 527, 632, 928
BstNSI RCATGY 3 cut(s) 55, 626, 793
BstSLI GKGCMC 2 cut(s) 40, 893
BstUI CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
BstV1I GCAGC 5 cut(s) 159, 267, 426, 459, 984
BstV2I GAAGAC 2 cut(s) 61, 343
BstZI CGGCCG 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 759
BsuRI GGCC 7 cut(s) 226, 360, 530, 604, 858, 891, 925
BtgZI GCGATG 1 cut(s) 626
BtrI CACGTC 1 cut(s) 886
BtsCI GGATG 1 cut(s) 489
BtuMI TCGCGA 1 cut(s) 1001
Cac8I GCNNGC 7 cut(s) 53, 165, 358, 681, 737, 812, 816
CfoI GCGC 5 cut(s) 420, 439, 472, 598, 795
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 9 cut(s) 522, 602, 664, 714, 840, 889, 890, 924, 966
Cfr9I CCCGGG 1 cut(s) 210
CseI GACGC 5 cut(s) 157, 337, 522, 617, 1028
Csp6I GTAC 4 cut(s) 517, 580, 871, 931
CviAII CATG 4 cut(s) 52, 259, 623, 790
CviQI GTAC 4 cut(s) 517, 580, 871, 931
DdeI CTNAG 2 cut(s) 759, 911
DpnI GATC 6 cut(s) 8, 450, 572, 647, 902, 987
DpnII GATC 6 cut(s) 6, 448, 570, 645, 900, 985
DraIII CACNNNGTG 1 cut(s) 865
EaeI YGGCCR 3 cut(s) 358, 528, 856
EagI CGGCCG 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 159, 536
EarI CTCTTC 2 cut(s) 159, 536
EciI GGCGGA 2 cut(s) 150, 400
EclXI CGGCCG 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 893
Eco47I GGWCC 5 cut(s) 522, 664, 714, 840, 966
Eco52I CGGCCG 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 689
Eco72I CACGTG 2 cut(s) 750, 832
Eco81I CCTNAGG 1 cut(s) 759
Eco88I CYCGRG 2 cut(s) 210, 220
EcoO109I RGGNCCY 1 cut(s) 664
EcoRII CCWGG 4 cut(s) 230, 525, 630, 926
EcoT38I GRGCYC 1 cut(s) 893
FaeI CATG 4 cut(s) 55, 262, 626, 793
FaiI YATR 6 cut(s) 44, 53, 260, 624, 791, 1043
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 1 cut(s) 758
FatI CATG 4 cut(s) 51, 258, 622, 789
FauI CCCGC 4 cut(s) 285, 388, 627, 1020
FblI GTMKAC 1 cut(s) 852
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 893
GlaI GCGC 5 cut(s) 419, 438, 471, 597, 794
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 653
HaeIII GGCC 7 cut(s) 226, 360, 530, 604, 858, 891, 925
HgaI GACGC 5 cut(s) 157, 337, 522, 617, 1028
HhaI GCGC 5 cut(s) 420, 439, 472, 598, 795
Hin1I GRCGYC 3 cut(s) 149, 348, 1053
Hin1II CATG 4 cut(s) 55, 262, 626, 793
Hin6I GCGC 5 cut(s) 418, 437, 470, 596, 793
HinP1I GCGC 5 cut(s) 418, 437, 470, 596, 793
HincII GTYRAC 3 cut(s) 217, 853, 952
HindII GTYRAC 3 cut(s) 217, 853, 952
HinfI GANTC 2 cut(s) 201, 321
HpaI GTTAAC 1 cut(s) 217
HphI GGTGA 7 cut(s) 64, 70, 259, 385, 706, 877, 955
Hpy166II GTNNAC 7 cut(s) 217, 307, 393, 835, 853, 946, 952
Hpy188I TCNGA 2 cut(s) 883, 1064
Hpy188III TCNNGA 4 cut(s) 238, 643, 667, 1000
Hpy8I GTNNAC 7 cut(s) 217, 307, 393, 835, 853, 946, 952
Hpy99I CGWCG 6 cut(s) 68, 449, 538, 857, 887, 941
HpyAV CCTTC 3 cut(s) 265, 295, 514
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 9 cut(s) 186, 303, 515, 749, 831, 869, 885, 948, 1053
HpyCH4V TGCA 2 cut(s) 51, 489
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 169, 685, 820, 978
HpyF3I CTNAG 2 cut(s) 759, 911
HpySE526I ACGT 9 cut(s) 186, 303, 515, 749, 831, 869, 885, 948, 1053
Hsp92I GRCGYC 3 cut(s) 149, 348, 1053
Hsp92II CATG 4 cut(s) 55, 262, 626, 793
HspAI GCGC 5 cut(s) 418, 437, 470, 596, 793
Kpn2I TCCGGA 1 cut(s) 642
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 6 cut(s) 6, 448, 570, 645, 900, 985
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 4 cut(s) 172, 295, 621, 732
Lsp1109I GCAGC 5 cut(s) 159, 267, 426, 459, 984
LweI GCATC 2 cut(s) 498, 603
MaeII ACGT 9 cut(s) 186, 303, 515, 749, 831, 869, 885, 948, 1053
MaeIII GTNAC 5 cut(s) 76, 698, 752, 865, 1049
MalI GATC 6 cut(s) 8, 450, 572, 647, 902, 987
MbiI CCGCTC 1 cut(s) 739
MboI GATC 6 cut(s) 6, 448, 570, 645, 900, 985
MboII GAAGA 5 cut(s) 61, 146, 343, 550, 553
MhlI GDGCHC 2 cut(s) 40, 893
MluCI AATT 1 cut(s) 766
MmeI TCCRAC 1 cut(s) 906
MroI TCCGGA 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 4 cut(s) 172, 280, 388, 706
MvaI CCWGG 4 cut(s) 232, 527, 632, 928
MvnI CGCG 8 cut(s) 418, 437, 470, 533, 598, 611, 795, 1001
MwoI GCNNNNNNNGC 5 cut(s) 10, 169, 685, 820, 978
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 6 cut(s) 211, 212, 362, 454, 844, 863
NdeII GATC 6 cut(s) 6, 448, 570, 645, 900, 985
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 4 cut(s) 55, 262, 626, 793
NlaIV GGNNCC 3 cut(s) 524, 665, 891
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 4 cut(s) 76, 698, 865, 1049
NruI TCGCGA 1 cut(s) 1001
NspI RCATGY 3 cut(s) 55, 626, 793
NspV TTCGAA 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 830
PaeI GCATGC 1 cut(s) 55
PaeR7I CTCGAG 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 7 cut(s) 72, 309, 441, 558, 579, 882, 945
PdiI GCCGGC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 201, 321
Pfl23II CGTACG 1 cut(s) 579
PflMI CCANNNNNTGG 1 cut(s) 963
PmaCI CACGTG 2 cut(s) 750, 832
PmlI CACGTG 2 cut(s) 750, 832
Ppu21I YACGTR 3 cut(s) 516, 750, 832
PpuMI RGGWCCY 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 4 cut(s) 230, 525, 630, 926
PspCI CACGTG 2 cut(s) 750, 832
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 4 cut(s) 230, 525, 630, 926
PspLI CGTACG 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 524, 665, 891
PspOMI GGGCCC 1 cut(s) 889
PspPI GGNCC 9 cut(s) 522, 602, 664, 714, 840, 889, 890, 924, 966
PspPPI RGGWCCY 1 cut(s) 664
PspXI VCTCGAGB 1 cut(s) 220
RruI TCGCGA 1 cut(s) 1001
RsaI GTAC 4 cut(s) 518, 581, 872, 932
RsaNI GTAC 4 cut(s) 517, 580, 871, 931
RseI CAYNNNNRTG 1 cut(s) 830
SalI GTCGAC 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 6 cut(s) 6, 448, 570, 645, 900, 985
Sau96I GGNCC 9 cut(s) 522, 602, 664, 714, 840, 889, 890, 924, 966
SduI GDGCHC 2 cut(s) 40, 893
SfaNI GCATC 2 cut(s) 498, 603
Sfr274I CTCGAG 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 5 cut(s) 522, 664, 714, 840, 966
SlaI CTCGAG 1 cut(s) 220
SmaI CCCGGG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
SphI GCATGC 1 cut(s) 55
Sse9I AATT 1 cut(s) 766
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 9 cut(s) 189, 306, 518, 752, 834, 872, 888, 951, 1056
TaqI TCGA 9 cut(s) 221, 374, 444, 461, 536, 552, 561, 852, 1026
TasI AATT 1 cut(s) 766
TauI GCSGC 6 cut(s) 209, 229, 423, 533, 596, 709
TfiI GAWTC 2 cut(s) 201, 321
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 4 cut(s) 76, 698, 865, 1049
TseI GCWGC 5 cut(s) 172, 280, 439, 472, 972
Tsp45I GTSAC 4 cut(s) 76, 698, 865, 1049
TspDTI ATGAA 3 cut(s) 247, 359, 538
TspGWI ACGGA 1 cut(s) 978
TspMI CCCGGG 1 cut(s) 210
Van91I CCANNNNNTGG 1 cut(s) 963
VpaK11BI GGWCC 5 cut(s) 522, 664, 714, 840, 966
XceI RCATGY 3 cut(s) 55, 626, 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 900
XhoI CTCGAG 1 cut(s) 220
XmaI CCCGGG 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 852
XmnI GAANNNNTTC 1 cut(s) 545
ZraI GACGTC 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.