MD00G1144500.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015487
Physical Location & Seq
Forward (+)
31661709 .. 31662833
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1144500.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGCTCTCCCCAACGCCTTTATCTCTCATCTGCGCCGTTCTTCTGTGTCTCCCTCTCACCGTAATCTTCACCATCAACAGCCCAACGGCCACAAAAACCACCGCCACCATTTCCCCCTCCCAAATCACAAAACCCTCCAAACCCCTAACAGACTTTAACCCAATTTCACCGCCAAAGTCCCTCCCTTTAGATGAAGACGCTCTCTTCCGCCTCGCCGCTCGGGTCAACTCGCGCCCCCCACGATCCGCCCGCCCCAAAATCGCCTTCCTCTTCCTCACCACCACCCCTCTCCCCTTCTCCCCTCTCTGGGAGCTCTTCTTCAACAAAATCCCCAAAACCCATTTCTCCATTTACGTCCACGCCGACCCGCGATTCCCCTACGACCCGCCGTTCTCCGGCGTGTTCGCGCACCGCGTTATCCCCTCCAAACCTGCCCAGCGGTTCACCTCCACGCTCATTTCGGCCGCGCGCCGCTTGCTCTCCCACGCGCTTCTCGGCGACAAAACGAACGCCATGTTTGCGCTCATTTCTCCCTCGTGCATCCCCCTCCACTCCTTCAACTTCACGTACCGGACGCTCGCCCGATCGAAGAAGAGTTTCATCGAGGTTCTGGATAACGAGATCGGGGCGTACGATAGATGGGCAGCGCGTGGGGCGGACGCGATGTTGCCGCAGGTGAAGCTGGAGGAGTTTCGGATCGGGTCCCAGTTTTGGATTCTGAAGCGGAAGCACGCGAGGATGGTGGTGGGCGACCACCGGCTTTGGTCCAAGTTCAAGCTACCGTGCGAGCGTTGGTACACGTGTTACCCCGAGGAAAATTACTTTCCTACCCTCCTCAACATGCGAGACCCCGGCGGGCTAGTGCCTGCCACGCTGACGCACGTGGACTGGCGGGGGAGGTTCGACGGCCACCCCCGCACGTACAACGCCTCCGAGGTGGGGCCCGAGTTGATACAATCGCTGAGGAACGACAGGCCGAGGTACGGCGACGAGGAGGAGAGCTGGAACGACACTGTTTTGACGGTGAGGGAACGGCGGGACCCGTTTCTGTTCGCGAGGAAGTTCCCGCCGGATGCGATCGGGTCGTTGATGAGTATGGCCAGTGACGTCATCTTCAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

375

Amino Acids

42.56

Weight (kDa)

9.61

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 87 - 322 1.6e-63 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 664
AatII GACGTC 1 cut(s) 1110
Acc36I ACCTGC 2 cut(s) 439, 664
AccBSI CCGCTC 1 cut(s) 218
AclWI GGATC 2 cut(s) 237, 704
AcoI YGGCCR 4 cut(s) 87, 462, 907, 1098
AcuI CTGAAG 1 cut(s) 740
AcvI CACGTG 2 cut(s) 801, 883
AcyI GRCGYC 1 cut(s) 1107
AfaI GTAC 5 cut(s) 569, 632, 797, 923, 983
AfiI CCNNNNNNNGG 6 cut(s) 306, 307, 395, 711, 939, 983
AflIII ACRYGT 2 cut(s) 798, 800
AgsI TTSAA 4 cut(s) 322, 559, 775, 1117
AjuI GAANNNNNNNTTGG 4 cut(s) 248, 280, 326, 358
AluBI AGCT 4 cut(s) 313, 682, 778, 1002
AluI AGCT 4 cut(s) 313, 682, 778, 1002
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 53, 840
AlwI GGATC 2 cut(s) 237, 704
Ama87I CYCGRG 3 cut(s) 219, 809, 944
AoxI GGCC 6 cut(s) 87, 462, 907, 941, 974, 1098
ApaI GGGCCC 1 cut(s) 945
ApeKI GCWGC 1 cut(s) 644
Asp700I GAANNNNTTC 1 cut(s) 596
AspLEI GCGC 8 cut(s) 35, 234, 409, 469, 471, 490, 523, 649
AspS9I GGNCC 5 cut(s) 702, 765, 941, 942, 1039
AsuC2I CCSGG 1 cut(s) 852
AsuHPI GGTGA 7 cut(s) 49, 61, 159, 268, 436, 688, 1036
AvaI CYCGRG 3 cut(s) 219, 809, 944
AvaII GGWCC 3 cut(s) 702, 765, 1039
BaeGI GKGCMC 1 cut(s) 945
BaeI ACNNNNGTAYC 2 cut(s) 787, 820
BalI TGGCCA 1 cut(s) 1100
BanII GRGCYC 2 cut(s) 315, 945
BauI CACGAG 1 cut(s) 535
BbrPI CACGTG 2 cut(s) 801, 883
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 962
BbvI GCAGC 1 cut(s) 656
BccI CCATC 3 cut(s) 80, 633, 733
BceAI ACGGC 6 cut(s) 20, 102, 373, 922, 1000, 1049
BcnI CCSGG 1 cut(s) 852
BcoDI GTCTC 2 cut(s) 53, 840
BfaI CTAG 1 cut(s) 860
BfuAI ACCTGC 2 cut(s) 439, 664
BisI GCNGC 5 cut(s) 216, 465, 472, 645, 671
BlsI GCNGC 5 cut(s) 217, 466, 473, 646, 672
Bme1390I CCNGG 1 cut(s) 852
Bme18I GGWCC 3 cut(s) 702, 765, 1039
BmeT110I CYCGRG 3 cut(s) 219, 809, 944
BmgT120I GGNCC 5 cut(s) 702, 765, 941, 942, 1039
BmiI GGNNCC 6 cut(s) 703, 704, 942, 943, 1040, 1041
BmrFI CCNGG 1 cut(s) 852
BmrI ACTGGG 1 cut(s) 700
BmsI GCATC 2 cut(s) 549, 1063
BmuI ACTGGG 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 852
BsaAI YACGTR 4 cut(s) 567, 801, 883, 921
BsaHI GRCGYC 1 cut(s) 1107
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 4 cut(s) 810, 850, 933, 977
BsaWI WCCGGW 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 6 cut(s) 306, 307, 395, 711, 939, 983
Bse118I RCCGGY 1 cut(s) 756
Bse1I ACTGG 3 cut(s) 706, 893, 1101
BseDI CCNNGG 4 cut(s) 810, 850, 933, 977
BseGI GGATG 3 cut(s) 540, 744, 1078
BseLI CCNNNNNNNGG 6 cut(s) 306, 307, 395, 711, 939, 983
BseMII CTCAG 1 cut(s) 953
BseNI ACTGG 3 cut(s) 706, 893, 1101
BsePI GCGCGC 1 cut(s) 467
BseRI GAGGAG 4 cut(s) 701, 824, 1007, 1010
BseSI GKGCMC 1 cut(s) 945
BseX3I CGGCCG 1 cut(s) 462
BseXI GCAGC 1 cut(s) 656
BseYI CCCAGC 1 cut(s) 435
Bsh1285I CGRYCG 3 cut(s) 465, 587, 1080
BshFI GGCC 6 cut(s) 89, 464, 909, 943, 976, 1100
BsiEI CGRYCG 3 cut(s) 465, 587, 1080
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 3 cut(s) 219, 809, 944
BsiSI CCGG 5 cut(s) 396, 571, 757, 852, 1070
BsiWI CGTACG 1 cut(s) 630
BslFI GGGAC 3 cut(s) 163, 688, 1052
BslI CCNNNNNNNGG 6 cut(s) 306, 307, 395, 711, 939, 983
BsmAI GTCTC 2 cut(s) 53, 840
BsmFI GGGAC 3 cut(s) 163, 688, 1052
BsnI GGCC 6 cut(s) 89, 464, 909, 943, 976, 1100
Bso31I GGTCTC 1 cut(s) 840
BsoBI CYCGRG 3 cut(s) 219, 809, 944
Bsp120I GGGCCC 1 cut(s) 941
Bsp1286I GDGCHC 2 cut(s) 315, 945
Bsp143I GATC 5 cut(s) 242, 584, 621, 696, 1077
Bsp68I TCGCGA 1 cut(s) 1055
BspANI GGCC 6 cut(s) 89, 464, 909, 943, 976, 1100
BspCNI CTCAG 1 cut(s) 954
BspLI GGNNCC 6 cut(s) 703, 704, 942, 943, 1040, 1041
BspMI ACCTGC 2 cut(s) 439, 664
BspPI GGATC 2 cut(s) 237, 704
BspQI GCTCTTC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 840
BsrBI CCGCTC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 756
BsrI ACTGG 3 cut(s) 706, 893, 1101
BssAI RCCGGY 1 cut(s) 756
BssECI CCNNGG 4 cut(s) 810, 850, 933, 977
BssHII GCGCGC 1 cut(s) 467
BssMI GATC 5 cut(s) 242, 584, 621, 696, 1077
BssNI GRCGYC 1 cut(s) 1107
BssSI CACGAG 1 cut(s) 535
Bst2BI CACGAG 1 cut(s) 535
Bst4CI ACNGT 4 cut(s) 61, 783, 1015, 1024
Bst6I CTCTTC 4 cut(s) 209, 275, 320, 587
BstACI GRCGYC 1 cut(s) 1107
BstBAI YACGTR 4 cut(s) 567, 801, 883, 921
BstC8I GCNNGC 8 cut(s) 250, 469, 476, 579, 732, 788, 857, 867
BstDEI CTNAG 1 cut(s) 962
BstF5I GGATG 3 cut(s) 540, 744, 1078
BstHHI GCGC 8 cut(s) 35, 234, 409, 469, 471, 490, 523, 649
BstKTI GATC 5 cut(s) 245, 587, 624, 699, 1080
BstMAI GTCTC 2 cut(s) 53, 840
BstMBI GATC 5 cut(s) 242, 584, 621, 696, 1077
BstMCI CGRYCG 3 cut(s) 465, 587, 1080
BstMWI GCNNNNNNNGC 6 cut(s) 475, 518, 653, 679, 871, 915
BstNSI RCATGY 1 cut(s) 844
BstSCI CCNGG 1 cut(s) 850
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 1 cut(s) 656
BstV2I GAAGAC 1 cut(s) 201
BstZI CGGCCG 1 cut(s) 462
BsuRI GGCC 6 cut(s) 89, 464, 909, 943, 976, 1100
BtgZI GCGATG 1 cut(s) 677
BtsCI GGATG 3 cut(s) 540, 744, 1078
BtsIMutI CAGTG 2 cut(s) 1011, 1108
BtuMI TCGCGA 1 cut(s) 1055
BveI ACCTGC 2 cut(s) 439, 664
Cac8I GCNNGC 8 cut(s) 250, 469, 476, 579, 732, 788, 857, 867
CfoI GCGC 8 cut(s) 35, 234, 409, 469, 471, 490, 523, 649
Cfr10I RCCGGY 1 cut(s) 756
Cfr13I GGNCC 5 cut(s) 702, 765, 941, 942, 1039
CseI GACGC 4 cut(s) 206, 583, 668, 886
Csp6I GTAC 5 cut(s) 568, 631, 796, 922, 982
CspCI CAANNNNNGTGG 2 cut(s) 743, 778
CviAII CATG 2 cut(s) 514, 841
CviQI GTAC 5 cut(s) 568, 631, 796, 922, 982
DdeI CTNAG 1 cut(s) 962
DpnI GATC 5 cut(s) 244, 586, 623, 698, 1079
DpnII GATC 5 cut(s) 242, 584, 621, 696, 1077
EaeI YGGCCR 4 cut(s) 87, 462, 907, 1098
EagI CGGCCG 1 cut(s) 462
Eam1104I CTCTTC 4 cut(s) 209, 275, 320, 587
EarI CTCTTC 4 cut(s) 209, 275, 320, 587
EciI GGCGGA 3 cut(s) 197, 235, 671
Ecl136II GAGCTC 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 462
Eco24I GRGCYC 2 cut(s) 315, 945
Eco31I GGTCTC 1 cut(s) 840
Eco47I GGWCC 3 cut(s) 702, 765, 1039
Eco52I CGGCCG 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 740
Eco72I CACGTG 2 cut(s) 801, 883
Eco88I CYCGRG 3 cut(s) 219, 809, 944
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 3 cut(s) 702, 941, 1039
EcoT38I GRGCYC 2 cut(s) 315, 945
FaeI CATG 2 cut(s) 517, 844
FaiI YATR 3 cut(s) 515, 842, 1097
FaqI GGGAC 3 cut(s) 163, 688, 1052
FatI CATG 2 cut(s) 513, 840
FauI CCCGC 8 cut(s) 257, 375, 393, 848, 885, 923, 1029, 1074
Fnu4HI GCNGC 5 cut(s) 216, 465, 472, 645, 671
FokI GGATG 3 cut(s) 527, 751, 1085
FriOI GRGCYC 2 cut(s) 315, 945
Fsp4HI GCNGC 5 cut(s) 216, 465, 472, 645, 671
FspBI CTAG 1 cut(s) 860
GlaI GCGC 8 cut(s) 34, 233, 408, 468, 470, 489, 522, 648
GluI GCNGC 5 cut(s) 216, 465, 472, 645, 671
GsaI CCCAGC 1 cut(s) 439
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 6 cut(s) 89, 464, 909, 943, 976, 1100
HapII CCGG 5 cut(s) 396, 571, 757, 852, 1070
HgaI GACGC 4 cut(s) 206, 583, 668, 886
HhaI GCGC 8 cut(s) 35, 234, 409, 469, 471, 490, 523, 649
Hin1I GRCGYC 1 cut(s) 1107
Hin1II CATG 2 cut(s) 517, 844
Hin6I GCGC 8 cut(s) 33, 232, 407, 467, 469, 488, 521, 647
HinP1I GCGC 8 cut(s) 33, 232, 407, 467, 469, 488, 521, 647
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 2 cut(s) 372, 715
HpaII CCGG 5 cut(s) 396, 571, 757, 852, 1070
HphI GGTGA 7 cut(s) 49, 61, 159, 268, 436, 688, 1036
Hpy166II GTNNAC 5 cut(s) 226, 358, 444, 798, 886
Hpy188I TCNGA 3 cut(s) 696, 720, 934
Hpy188III TCNNGA 2 cut(s) 611, 1054
Hpy8I GTNNAC 5 cut(s) 226, 358, 444, 798, 886
Hpy99I CGWCG 2 cut(s) 908, 992
HpyAV CCTTC 3 cut(s) 274, 304, 565
HpyCH4III ACNGT 4 cut(s) 61, 783, 1015, 1024
HpyCH4IV ACGT 6 cut(s) 354, 566, 800, 882, 920, 1107
HpyCH4V TGCA 1 cut(s) 540
HpyF10VI GCNNNNNNNGC 6 cut(s) 475, 518, 653, 679, 871, 915
HpyF3I CTNAG 1 cut(s) 962
HpySE526I ACGT 6 cut(s) 354, 566, 800, 882, 920, 1107
Hsp92I GRCGYC 1 cut(s) 1107
Hsp92II CATG 2 cut(s) 517, 844
HspAI GCGC 8 cut(s) 33, 232, 407, 467, 469, 488, 521, 647
KflI GGGWCCC 2 cut(s) 702, 1039
Kzo9I GATC 5 cut(s) 242, 584, 621, 696, 1077
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 656
LweI GCATC 2 cut(s) 549, 1063
MaeI CTAG 1 cut(s) 860
MaeII ACGT 6 cut(s) 354, 566, 800, 882, 920, 1107
MaeIII GTNAC 2 cut(s) 803, 1103
MalI GATC 5 cut(s) 244, 586, 623, 698, 1079
MbiI CCGCTC 1 cut(s) 218
MboI GATC 5 cut(s) 242, 584, 621, 696, 1077
MhlI GDGCHC 2 cut(s) 315, 945
MlsI TGGCCA 1 cut(s) 1100
MluCI AATT 2 cut(s) 162, 817
MluNI TGGCCA 1 cut(s) 1100
Mox20I TGGCCA 1 cut(s) 1100
MroXI GAANNNNTTC 1 cut(s) 596
MscI TGGCCA 1 cut(s) 1100
MseI TTAA 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 1100
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 5 cut(s) 396, 571, 757, 852, 1070
MspR9I CCNGG 1 cut(s) 852
MwoI GCNNNNNNNGC 6 cut(s) 475, 518, 653, 679, 871, 915
NciI CCSGG 1 cut(s) 852
NdeII GATC 5 cut(s) 242, 584, 621, 696, 1077
NlaIII CATG 2 cut(s) 517, 844
NlaIV GGNNCC 6 cut(s) 703, 704, 942, 943, 1040, 1041
NmeAIII GCCGAG 2 cut(s) 474, 1002
NmuCI GTSAC 1 cut(s) 1103
NruI TCGCGA 1 cut(s) 1055
NspI RCATGY 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 664
PauI GCGCGC 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 3 cut(s) 360, 411, 630
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 372, 715
Pfl23II CGTACG 1 cut(s) 630
PkrI GCNGC 5 cut(s) 217, 466, 473, 646, 672
Ple19I CGATCG 2 cut(s) 587, 1080
PmaCI CACGTG 2 cut(s) 801, 883
PmlI CACGTG 2 cut(s) 801, 883
Ppu21I YACGTR 4 cut(s) 567, 801, 883, 921
PpuMI RGGWCCY 2 cut(s) 702, 1039
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 2 cut(s) 702, 1039
PspCI CACGTG 2 cut(s) 801, 883
PspFI CCCAGC 1 cut(s) 435
PspLI CGTACG 1 cut(s) 630
PspN4I GGNNCC 6 cut(s) 703, 704, 942, 943, 1040, 1041
PspOMI GGGCCC 1 cut(s) 941
PspPI GGNCC 5 cut(s) 702, 765, 941, 942, 1039
PspPPI RGGWCCY 2 cut(s) 702, 1039
PteI GCGCGC 1 cut(s) 467
PvuI CGATCG 2 cut(s) 587, 1080
RruI TCGCGA 1 cut(s) 1055
RsaI GTAC 5 cut(s) 569, 632, 797, 923, 983
RsaNI GTAC 5 cut(s) 568, 631, 796, 922, 982
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 5 cut(s) 216, 465, 472, 645, 671
Sau3AI GATC 5 cut(s) 242, 584, 621, 696, 1077
Sau96I GGNCC 5 cut(s) 702, 765, 941, 942, 1039
ScrFI CCNGG 1 cut(s) 852
SduI GDGCHC 2 cut(s) 315, 945
SfaNI GCATC 2 cut(s) 549, 1063
SinI GGWCC 3 cut(s) 702, 765, 1039
Sse9I AATT 2 cut(s) 162, 817
SspMI CTAG 1 cut(s) 860
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 850
TaaI ACNGT 4 cut(s) 61, 783, 1015, 1024
TaiI ACGT 6 cut(s) 357, 569, 803, 885, 923, 1110
TaqI TCGA 3 cut(s) 587, 603, 903
TasI AATT 2 cut(s) 162, 817
TauI GCSGC 4 cut(s) 218, 467, 474, 673
TfiI GAWTC 2 cut(s) 372, 715
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 1018, 1108
TseFI GTSAC 1 cut(s) 1103
TseI GCWGC 1 cut(s) 644
Tsp45I GTSAC 1 cut(s) 1103
TspDTI ATGAA 2 cut(s) 207, 589
TspRI CASTG 2 cut(s) 1018, 1108
VpaK11BI GGWCC 3 cut(s) 702, 765, 1039
XceI RCATGY 1 cut(s) 844
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 1 cut(s) 860
ZraI GACGTC 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.