FvH4_2g29000

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015487
Physical Location & Seq
Reverse (-)
22601439 .. 22602509
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29000.t1

Sequence Viewer

Length: 1071 bp
ATGCCCCTCGCCGTCGTCTTCACCGTCACCAGCCCCACCACCGTTTCTATCCAATCCCCCAAATCGTCGACCATAACCCACCGCAAACTAAACCTGACGCCACTCCTGTCCCCGCCTCCGCCGCCTCCGCTGCCGTTGGATGATGTATCTCTTTTACGAATCGCGGCGAGGGTTAACTCCAGGCCGCCGCGGTTCAAGAAGCCGAAAATCGCGTTCATGTTTCTCACCACCACGCCGCTGCCGTTCGCTCCGCTTTGGGAGGAGTTCTTTAGAGACGCCCCGAAATCGCTGTACAGAGTATACGTCCACGCCGATCCCAGATTCCCATACGAGCCTCCGTTCTCCGGCGTGTTCGCCGGGAGAGTGATTCATTCCAAGCCGGCGCAGCGGTTCACCCCGACGCTCGTCTCCGCCACGCGCCGCCTCCTCGCGCACGCGCTGCTTGACGATCCGATGAACTCGATGTTCGCTCTGTTGACACCGTCGTGCATCCCCCTCCACTCGTTCAACTTCACGTACCGGACGCTGGCCGCGTCGAGGAGGAGCTTCATCGAGATCCTCGAGAACGAGGTCGGGGCGTACGACAGGTGGGCGGCGCGTGGGGAGGACGCGATGCTGCCGCAGGTGAGCCTGGAGGAGTTCCGGATCGGGTCGCAGTTTTGGGTGCTGAAGCGGAAGCACGCCCGGCTGGTGGTGAGCGACCAGCGGCTTTGGTCCAAGTTCAAGCTGCCCTGCGAGCGCTGGGACACGTGTTACCCGGAGGAAAATTACTTCCCTACCCTCCTCCACATGCGCGACCCTGGCGGGTGCGTGCCCGCCACGCTCACGCACGTGGACTGGAGCGGCAGGTTCGACGGCCACCCGGTGACTTACAACGCCACCGACGTGGGGCCCGACCTGATATGGTCGCTGAGGGAGGAGAGGCCCAGGTACGGCGACGAGGTGAACGTCAACGCCACCGAGTGGTCCGGCAGCGAACGCCACGATCCGTTCCTTTTCGCGAGGAAGTTTCCGCCGGAAGCAATCGAGCCGTTGATGAGTATGGCGAGTGACGTCATTTTCCGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

357

Amino Acids

40.68

Weight (kDa)

8.84

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 70 - 303 4.4e-65 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32290
fragaria_vesca FvH4_2g29000
malus_domestica MD00G1144500.v1.1
prunus_persica Prupe.7G026200_v2.0.a1
pyrus_communis pycom15g11340
rosa_chinensis RchiOBHm_Chr6g0297881
rosa_laevigata RLG00000011554
rosa_multiflora Rmu_co8427971.1_g000001 Rmu_sc0019614.1_g000002
rosa_roxburghii Rroxscaffold_7G00170120
rosa_rugosa Rorug06G0276500
rosa_samantha Rh6AG387000 Rh6BG395500 Rh6CG401300 Rh6DG387800
rosa_wichuraiana Rw6G033720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
AatII GACGTC 1 cut(s) 1056
Acc36I ACCTGC 2 cut(s) 613, 837
AccB7I CCANNNNNTGG 1 cut(s) 963
AccBSI CCGCTC 1 cut(s) 843
AccI GTMKAC 2 cut(s) 68, 300
AccIII TCCGGA 1 cut(s) 642
AclWI GGATC 5 cut(s) 308, 443, 550, 653, 980
AcoI YGGCCR 2 cut(s) 528, 856
AcuI CTGAAG 1 cut(s) 689
AcvI CACGTG 2 cut(s) 750, 832
AcyI GRCGYC 3 cut(s) 98, 276, 1053
AdeI CACNNNGTG 2 cut(s) 865, 963
AfaI GTAC 4 cut(s) 293, 518, 581, 932
AfeI AGCGCT 1 cut(s) 740
AfiI CCNNNNNNNGG 7 cut(s) 344, 526, 537, 691, 888, 932, 963
AflIII ACRYGT 2 cut(s) 747, 749
AgsI TTSAA 3 cut(s) 196, 508, 724
AjiI CACGTC 1 cut(s) 886
AjnI CCWGG 4 cut(s) 179, 630, 799, 926
AleI CACNNNNGTG 3 cut(s) 484, 830, 884
AluBI AGCT 2 cut(s) 546, 727
AluI AGCT 2 cut(s) 546, 727
Alw26I GTCTC 2 cut(s) 267, 412
AlwI GGATC 5 cut(s) 308, 443, 550, 653, 980
Ama87I CYCGRG 1 cut(s) 560
Aor13HI TCCGGA 1 cut(s) 642
Aor51HI AGCGCT 1 cut(s) 740
AoxI GGCC 5 cut(s) 182, 528, 856, 890, 923
ApaI GGGCCC 1 cut(s) 894
ApeKI GCWGC 7 cut(s) 130, 238, 385, 439, 616, 727, 972
AspLEI GCGC 7 cut(s) 385, 420, 433, 439, 598, 741, 795
AspS9I GGNCC 5 cut(s) 714, 890, 891, 924, 966
AsuC2I CCSGG 4 cut(s) 358, 685, 758, 863
AsuHPI GGTGA 8 cut(s) 13, 19, 217, 385, 637, 706, 877, 955
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 2 cut(s) 714, 966
BaeGI GKGCMC 2 cut(s) 816, 894
BanII GRGCYC 1 cut(s) 894
BbrPI CACGTG 2 cut(s) 750, 832
BbsI GAAGAC 1 cut(s) 10
BbvCI CCTCAGC 1 cut(s) 911
BbvI GCAGC 7 cut(s) 117, 225, 397, 426, 603, 714, 984
BceAI ACGGC 5 cut(s) 118, 226, 871, 949, 1015
BciT130I CCWGG 4 cut(s) 181, 632, 801, 928
BcnI CCSGG 4 cut(s) 358, 685, 758, 863
BcoDI GTCTC 2 cut(s) 267, 412
BfoI RGCGCY 1 cut(s) 742
BfuAI ACCTGC 2 cut(s) 613, 837
Bme1390I CCNGG 8 cut(s) 181, 358, 632, 685, 758, 801, 863, 928
Bme18I GGWCC 2 cut(s) 714, 966
BmeT110I CYCGRG 1 cut(s) 560
BmgBI CACGTC 1 cut(s) 886
BmgT120I GGNCC 5 cut(s) 714, 890, 891, 924, 966
BmiI GGNNCC 2 cut(s) 891, 892
BmrFI CCNGG 8 cut(s) 181, 358, 632, 685, 758, 801, 863, 928
BmsI GCATC 2 cut(s) 498, 603
BoxI GACNNNNGTC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 10
BpmI CTGGAG 3 cut(s) 163, 653, 859
Bpu10I CCTNAGC 1 cut(s) 911
BpuMI CCSGG 4 cut(s) 358, 685, 758, 863
BsaAI YACGTR 3 cut(s) 516, 750, 832
BsaHI GRCGYC 3 cut(s) 98, 276, 1053
BsaJI CCNNGG 3 cut(s) 188, 799, 926
BsaWI WCCGGW 2 cut(s) 519, 642
Bsc4I CCNNNNNNNGG 7 cut(s) 344, 526, 537, 691, 888, 932, 963
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 842
BseAI TCCGGA 1 cut(s) 642
BseBI CCWGG 4 cut(s) 181, 632, 801, 928
BseDI CCNNGG 3 cut(s) 188, 799, 926
BseGI GGATG 2 cut(s) 145, 489
BseLI CCNNNNNNNGG 7 cut(s) 344, 526, 537, 691, 888, 932, 963
BseMII CTCAG 1 cut(s) 902
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 7 cut(s) 275, 416, 553, 556, 650, 773, 932
BseSI GKGCMC 2 cut(s) 816, 894
BseXI GCAGC 7 cut(s) 117, 225, 397, 426, 603, 714, 984
BseYI CCCAGC 1 cut(s) 741
BshFI GGCC 5 cut(s) 184, 530, 858, 892, 925
BsiHKCI CYCGRG 1 cut(s) 560
BsiWI CGTACG 1 cut(s) 579
BslFI GGGAC 2 cut(s) 94, 758
BslI CCNNNNNNNGG 7 cut(s) 344, 526, 537, 691, 888, 932, 963
BsmAI GTCTC 2 cut(s) 267, 412
BsmBI CGTCTC 2 cut(s) 267, 412
BsmFI GGGAC 2 cut(s) 94, 758
BsnI GGCC 5 cut(s) 184, 530, 858, 892, 925
BsoBI CYCGRG 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 890
Bsp1286I GDGCHC 2 cut(s) 816, 894
Bsp13I TCCGGA 1 cut(s) 642
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 5 cut(s) 313, 448, 555, 645, 985
Bsp68I TCGCGA 1 cut(s) 1001
BspANI GGCC 5 cut(s) 184, 530, 858, 892, 925
BspCNI CTCAG 1 cut(s) 903
BspEI TCCGGA 1 cut(s) 642
BspLI GGNNCC 2 cut(s) 891, 892
BspMI ACCTGC 2 cut(s) 613, 837
BspPI GGATC 5 cut(s) 308, 443, 550, 653, 980
BsrBI CCGCTC 1 cut(s) 843
BsrFI RCCGGY 1 cut(s) 379
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 1 cut(s) 842
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 3 cut(s) 188, 799, 926
BssMI GATC 5 cut(s) 313, 448, 555, 645, 985
BssNAI GTATAC 1 cut(s) 301
BssNI GRCGYC 3 cut(s) 98, 276, 1053
Bst1107I GTATAC 1 cut(s) 301
Bst2UI CCWGG 4 cut(s) 181, 632, 801, 928
Bst4CI ACNGT 3 cut(s) 25, 43, 483
BstACI GRCGYC 3 cut(s) 98, 276, 1053
BstAPI GCANNNNNTGC 1 cut(s) 439
BstAUI TGTACA 1 cut(s) 291
BstBAI YACGTR 3 cut(s) 516, 750, 832
BstC8I GCNNGC 7 cut(s) 381, 435, 528, 681, 737, 812, 816
BstDEI CTNAG 1 cut(s) 911
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 2 cut(s) 145, 489
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 7 cut(s) 385, 420, 433, 439, 598, 741, 795
BstKTI GATC 5 cut(s) 316, 451, 558, 648, 988
BstMAI GTCTC 2 cut(s) 267, 412
BstMBI GATC 5 cut(s) 313, 448, 555, 645, 985
BstNI CCWGG 4 cut(s) 181, 632, 801, 928
BstNSI RCATGY 1 cut(s) 793
BstPAI GACNNNNGTC 1 cut(s) 404
BstSCI CCNGG 8 cut(s) 179, 356, 630, 683, 756, 799, 861, 926
BstSLI GKGCMC 2 cut(s) 816, 894
BstV1I GCAGC 7 cut(s) 117, 225, 397, 426, 603, 714, 984
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 555
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 1 cut(s) 555
BstZ17I GTATAC 1 cut(s) 301
BsuRI GGCC 5 cut(s) 184, 530, 858, 892, 925
BtgI CCRYGG 1 cut(s) 188
BtgZI GCGATG 1 cut(s) 626
BtrI CACGTC 1 cut(s) 886
BtsCI GGATG 2 cut(s) 145, 489
BtuMI TCGCGA 1 cut(s) 1001
BveI ACCTGC 2 cut(s) 613, 837
Cac8I GCNNGC 7 cut(s) 381, 435, 528, 681, 737, 812, 816
CfoI GCGC 7 cut(s) 385, 420, 433, 439, 598, 741, 795
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 5 cut(s) 714, 890, 891, 924, 966
Cfr42I CCGCGG 1 cut(s) 191
CseI GACGC 6 cut(s) 106, 284, 409, 522, 532, 617
Csp6I GTAC 4 cut(s) 292, 517, 580, 931
CviAII CATG 2 cut(s) 217, 790
CviQI GTAC 4 cut(s) 292, 517, 580, 931
DdeI CTNAG 1 cut(s) 911
DpnI GATC 5 cut(s) 315, 450, 557, 647, 987
DpnII GATC 5 cut(s) 313, 448, 555, 645, 985
DraIII CACNNNGTG 2 cut(s) 865, 963
EaeI YGGCCR 2 cut(s) 528, 856
EciI GGCGGA 3 cut(s) 108, 400, 1002
Eco24I GRGCYC 1 cut(s) 894
Eco47I GGWCC 2 cut(s) 714, 966
Eco47III AGCGCT 1 cut(s) 740
Eco57I CTGAAG 1 cut(s) 689
Eco72I CACGTG 2 cut(s) 750, 832
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 890
EcoRII CCWGG 4 cut(s) 179, 630, 799, 926
EcoT38I GRGCYC 1 cut(s) 894
Esp3I CGTCTC 2 cut(s) 267, 412
FaeI CATG 2 cut(s) 220, 793
FaiI YATR 7 cut(s) 74, 218, 301, 328, 791, 904, 1043
FaqI GGGAC 2 cut(s) 94, 758
FatI CATG 2 cut(s) 216, 789
FauI CCCGC 3 cut(s) 120, 797, 823
FblI GTMKAC 2 cut(s) 68, 300
FokI GGATG 2 cut(s) 152, 476
FriOI GRGCYC 1 cut(s) 894
GlaI GCGC 7 cut(s) 384, 419, 432, 438, 597, 740, 794
GsaI CCCAGC 1 cut(s) 745
GsuI CTGGAG 3 cut(s) 163, 653, 859
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 5 cut(s) 184, 530, 858, 892, 925
HgaI GACGC 6 cut(s) 106, 284, 409, 522, 532, 617
HhaI GCGC 7 cut(s) 385, 420, 433, 439, 598, 741, 795
Hin1I GRCGYC 3 cut(s) 98, 276, 1053
Hin1II CATG 2 cut(s) 220, 793
Hin6I GCGC 7 cut(s) 383, 418, 431, 437, 596, 739, 793
HinP1I GCGC 7 cut(s) 383, 418, 431, 437, 596, 739, 793
HincII GTYRAC 4 cut(s) 69, 175, 477, 952
HindII GTYRAC 4 cut(s) 69, 175, 477, 952
HinfI GANTC 3 cut(s) 159, 321, 367
HpaI GTTAAC 1 cut(s) 175
HphI GGTGA 8 cut(s) 13, 19, 217, 385, 637, 706, 877, 955
Hpy166II GTNNAC 9 cut(s) 69, 175, 301, 307, 393, 477, 835, 946, 952
Hpy188I TCNGA 2 cut(s) 453, 1064
Hpy188III TCNNGA 5 cut(s) 196, 553, 562, 643, 1000
Hpy8I GTNNAC 9 cut(s) 69, 175, 301, 307, 393, 477, 835, 946, 952
Hpy99I CGWCG 8 cut(s) 17, 70, 403, 487, 538, 857, 887, 941
HpyCH4III ACNGT 3 cut(s) 25, 43, 483
HpyCH4IV ACGT 7 cut(s) 303, 515, 749, 831, 885, 948, 1053
HpyCH4V TGCA 1 cut(s) 489
HpyF3I CTNAG 1 cut(s) 911
HpySE526I ACGT 7 cut(s) 303, 515, 749, 831, 885, 948, 1053
Hsp92I GRCGYC 3 cut(s) 98, 276, 1053
Hsp92II CATG 2 cut(s) 220, 793
HspAI GCGC 7 cut(s) 383, 418, 431, 437, 596, 739, 793
Kpn2I TCCGGA 1 cut(s) 642
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 175
KspI CCGCGG 1 cut(s) 191
Kzo9I GATC 5 cut(s) 313, 448, 555, 645, 985
LmnI GCTCC 3 cut(s) 253, 543, 840
Lsp1109I GCAGC 7 cut(s) 117, 225, 397, 426, 603, 714, 984
LweI GCATC 2 cut(s) 498, 603
MaeII ACGT 7 cut(s) 303, 515, 749, 831, 885, 948, 1053
MaeIII GTNAC 4 cut(s) 25, 752, 865, 1049
MalI GATC 5 cut(s) 315, 450, 557, 647, 987
MbiI CCGCTC 1 cut(s) 843
MboI GATC 5 cut(s) 313, 448, 555, 645, 985
MboII GAAGA 1 cut(s) 10
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 2 cut(s) 816, 894
MluCI AATT 1 cut(s) 766
MmeI TCCRAC 1 cut(s) 117
MroI TCCGGA 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 379
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 3 cut(s) 484, 830, 884
MspA1I CMGCKG 5 cut(s) 130, 190, 238, 388, 706
MspR9I CCNGG 8 cut(s) 181, 358, 632, 685, 758, 801, 863, 928
MvaI CCWGG 4 cut(s) 181, 632, 801, 928
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 4 cut(s) 358, 685, 758, 863
NdeII GATC 5 cut(s) 313, 448, 555, 645, 985
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 2 cut(s) 220, 793
NlaIV GGNNCC 2 cut(s) 891, 892
NmuCI GTSAC 3 cut(s) 25, 865, 1049
NruI TCGCGA 1 cut(s) 1001
NspI RCATGY 1 cut(s) 793
OliI CACNNNNGTG 3 cut(s) 484, 830, 884
PaeR7I CTCGAG 1 cut(s) 560
PaqCI CACCTGC 1 cut(s) 613
PcsI WCGNNNNNNNCGW 9 cut(s) 21, 239, 309, 530, 558, 579, 882, 891, 945
PdiI GCCGGC 1 cut(s) 381
PfeI GAWTC 3 cut(s) 159, 321, 367
Pfl23II CGTACG 1 cut(s) 579
PflFI GACNNNGTC 1 cut(s) 481
PflMI CCANNNNNTGG 1 cut(s) 963
PmaCI CACGTG 2 cut(s) 750, 832
PmlI CACGTG 2 cut(s) 750, 832
Ppu21I YACGTR 3 cut(s) 516, 750, 832
PshAI GACNNNNGTC 1 cut(s) 404
Psp6I CCWGG 4 cut(s) 179, 630, 799, 926
PspCI CACGTG 2 cut(s) 750, 832
PspFI CCCAGC 1 cut(s) 741
PspGI CCWGG 4 cut(s) 179, 630, 799, 926
PspLI CGTACG 1 cut(s) 579
PspN4I GGNNCC 2 cut(s) 891, 892
PspOMI GGGCCC 1 cut(s) 890
PspPI GGNCC 5 cut(s) 714, 890, 891, 924, 966
PsuI RGATCY 1 cut(s) 555
PsyI GACNNNGTC 1 cut(s) 481
RruI TCGCGA 1 cut(s) 1001
RsaI GTAC 4 cut(s) 293, 518, 581, 932
RsaNI GTAC 4 cut(s) 292, 517, 580, 931
RseI CAYNNNNRTG 3 cut(s) 484, 830, 884
SacII CCGCGG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 67
SaqAI TTAA 1 cut(s) 174
Sau3AI GATC 5 cut(s) 313, 448, 555, 645, 985
Sau96I GGNCC 5 cut(s) 714, 890, 891, 924, 966
ScrFI CCNGG 8 cut(s) 181, 358, 632, 685, 758, 801, 863, 928
SduI GDGCHC 2 cut(s) 816, 894
SfaNI GCATC 2 cut(s) 498, 603
Sfr274I CTCGAG 1 cut(s) 560
Sfr303I CCGCGG 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 191
SinI GGWCC 2 cut(s) 714, 966
SlaI CTCGAG 1 cut(s) 560
SmiMI CAYNNNNRTG 3 cut(s) 484, 830, 884
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 1 cut(s) 766
StyD4I CCNGG 8 cut(s) 179, 356, 630, 683, 756, 799, 861, 926
TaaI ACNGT 3 cut(s) 25, 43, 483
TaiI ACGT 7 cut(s) 306, 518, 752, 834, 888, 951, 1056
TaqI TCGA 7 cut(s) 68, 461, 536, 552, 561, 852, 1026
TasI AATT 1 cut(s) 766
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 3 cut(s) 159, 321, 367
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TseFI GTSAC 3 cut(s) 25, 865, 1049
TseI GCWGC 7 cut(s) 130, 238, 385, 439, 616, 727, 972
Tsp45I GTSAC 3 cut(s) 25, 865, 1049
TspDTI ATGAA 4 cut(s) 205, 359, 470, 538
TspGWI ACGGA 2 cut(s) 327, 978
Tth111I GACNNNGTC 1 cut(s) 481
Van91I CCANNNNNTGG 1 cut(s) 963
VpaK11BI GGWCC 2 cut(s) 714, 966
XceI RCATGY 1 cut(s) 793
XhoI CTCGAG 1 cut(s) 560
XmiI GTMKAC 2 cut(s) 68, 300
ZraI GACGTC 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.