AT4G32700

Helicase and polymerase-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013097
Physical Location & Seq
Forward (+)
15767185 .. 15779697
12513 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32700.2

Sequence Viewer

Length: 6465 bp
ATGGACTCAGATTCTTCTAAATCGCGGATTGATCAGTTTTATGTATCGAAGAAGAGGAAACACCAGTCACCAAATTTAAAGTCTGGGAGGAATGAGAAAAATGTGAAGGTGACAGGGGAAAGATCTCCTGGAGACAAAGGCACTTTGGACAGTTACTTGAAAGCCTCTCTGGATGACAAGAGTACTACCAATAGTGGGTTGCAGGCAAGGCAGGAAGCGTTTACAAGAAAATTGGATTTGGAGGTTTCTGCTTCGTCTGTTGGTCAAAATATACACCCTTGCCTGCCTAAACCTGTAAGTTTTGCAACTTTTAAGGAGTGTTTGGGCCAGAACGGATCTCAAGATTTGCATAAAGAAGGTGTTGCAGCTGAAACTCATGCTACAGATGGTTTACTCTGTGCTAACCAGAAAGATAATTCAGAACTACGAGACTTTGCCACTAGTTTCTTGTCCTTGTATTGTAGTGGAGTGCAGTCTGTTGTTGGCTCACCTCCACATCAGAAGGAAAATGAATTGAAGCGTCGAAGCAGTTCATCATCACTTGCCCAAGATATACAAATTTCTCACAAGAGGCGTTGTGAGTCCGAAAATATACCATCACTAGATGATCTTACAAATCCTCTCGGGAGTAAGCCTGAATCACTCGCTAGGAATGGTAATAATAGAGATAAACCTGTTTCAGACCCTACAAAGAAGATGCCGAGCAATGAATCTGTTGAAATTCCAATGGGCTTGAGGAAGTGTAGTAAGGCGCCAGAATCATCTGCTCATCTAACTGAATTCCACACGCCTGGATCAGCCATTAAATCATGTCCTGTTGGAACCCCTAAGTCAGGATGTGGTAGCTCAATGTTCTCACCTGGTGAGGCTTTCTGGAATGAAGCAATTCAAGTTGCTGATGGTTTGACAATTCCAATTGAAAATTTTGGTTCTGTAGAAGCTAAAGTTAGAGATCAACATGTAACTATTCTTTCATGCAGCAAGAAAACAGATAAATGCACTGAAAAGTTAGAGAGGAGTTTAGATTTAGATGAAATCAGAGTAAAGGATAAAGATGCCATTGGTTTTTCAAAGGTAGTGGAGAAGCATGGGAGAGATTTTAATAAAGAAGTATATCAGCTTCCTGTCAAGAATCTTGAACTTTTGTTTCAAGATAAGAACATTAATGGAGGTATTCAAGAGCGATGTGCTTCATTTGATCAAAATAACATTACTCTAGGAAGCAGTAGGATCTCTGAATCTGCTTTCGTCGGTAATAAAGGATGTGAAAATCTTGATATTGCAAATAATGCTCAGGCAGATAAAGGTTTGATAGGCAAGATGTATCCAGAACCCGAAGGAAAGAAAGTTTTACTGTGTGAGGAAAATCGTGGTGTAAGATCTGTTTCTATGATAAGCAACATGAGGAAACCAGTTGGTTCTAGTGAATCCGAAGAAAGTCATACTCCTTCAAGCTCTCATCGTAATTACGATGGCTTGAGTCTCAGCACTTGGCTTCCCTCAGAAGTTTGCAGTGTATACAATAAGAAAGGAATATCTAAACTCTATCCTTGGCAGGTCGAGTGTCTTCAGGTAGATGGTGTTTTGCAGAAAAGGAATCTAGTATATTGTGCTTCTACAAGTGCTGGTAAAAGTTTTGTTGCAGAAGTCTTGATGCTGCGCCGGGTCATAAGAACTGGAAAAATGGCATTGCTTGTACTACCTTATGTATCCATTTGTGCCGAGAAGGCGGAACACCTTGAGGTCCTTCTTGAACCACTTGGTAAGCATGTTCGTAGTTACTATGGAAATCAGGGTGGTGGAACACTTCCTAAAGACACTTCTGTGGCTGTCTGCACGATTGAGAAGGCAAACTCTTTGATAAATCGCTTGCTTGAAGAGGGTCGTTTGTCAGAACTTGGAATAATAGTTATAGATGAGCTACATATGGTGGGTGACCAACATAGGGGTTATCTTCTGGAACTTATGTTGACGAAACTTCGTTATGCTGCTGGTGAAGGCAGTTCAGAATCAAGTAGTGGTGAGAGTTCAGGGACTAGCAGTGGCAAAGCTGATCCTGCTCATGGCCTACAAATTGTGGGTATGAGTGCTACAATGCCAAATGTTGGCGCAGTTGCTGACTGGCTTCAAGCAGCTTTATATCAGACAGAGTTTCGACCAGTTCCCCTGGAGGAGTATATTAAAGTAGGTAGCACCATTTACAATAAAAAGATGGAAGTTGTGAGGACTATACCAAAAGCTGCTGATATGGGAGGGAAAGACCCGGACCATATAGTTGAGCTTTGTAACGAGGTTGTTCAAGAGGGCAATTCAGTGCTGATATTTTGCTCGAGTCGAAAAGGATGTGAATCAACTGCTAGGCATATTTCAAAGCTCATTAAAAATGTTCCCGTAAACGTTGATGGTGAAAATAGTGAGTTTATGGATATCAGATCTGCTATTGATGCCCTGCGAAGGTCTCCGTCTGGAGTAGATCCTGTACTAGAAGAAACTCTTCCCTCTGGGGTTGCCTATCACCATGCTGGTCTTACAGTAGAAGAAAGAGAGATAGTTGAGACCTGTTACCGTAAGGGTCTTGTACGTGTCTTAACAGCTACATCCACTTTGGCGGCTGGGGTTAACCTGCCTGCAAGGAGGGTTATTTTTCGACAACCAATGATTGGCCGTGATTTTATTGATGGAACAAGGTACAAGCAAATGTCTGGTCGAGCTGGGCGAACTGGGATTGATACAAAAGGAGATAGTGTTTTGATTTGCAAACCTGGAGAACTCAAGCGAATAATGGCACTTCTTAATGAAACCTGCCCACCATTGCAGTCTTGTTTATCTGAGGATAAAAATGGAATGACTCATGCAATACTAGAAGTTGTGGCAGGTGGAATTGTTCAGACTGCCAAAGATATTCATCGCTATGTTAGGTGTACACTTCTGAATTCTACAAAGCCCTTTCAAGATGTGGTCAAATCAGCTCAGGATTCTCTTCGGTGGCTGTGCCACAGGAAATTTCTTGAGTGGAATGAAGAGACAAAGTTATACACTACAACACCCCTTGGACGTGGATCTTTTGGCAGTTCACTCTGCCCAGAGGAGTCACTTATAGTGCTGGATGATCTTCTACGAGCACGTGAAGGACTAGTTATGGCTTCTGATTTGCATCTGGTCTACTTAGTCACACCAATTAATGTTGGTGTTGAGCCAAACTGGGAATTGTATTATGAACGGTTCATGGAACTGTCTCCTTTAGAGCAGTCTGTTGGCAATAGAGTTGGAGTGGTCGAACCTTTCCTGATGCGCATGGCACATGGTGCAACAGTTCGCACTCTAAACAGACCACAGGATGTAAAAAAAAATTTGCGTGGAGAATATGACAGTCGGCATGGTTCGACAAGCATGAAGATGCTTTCAGATGAACAAATGCTTCGAGTGTGCAAAAGATTTTTTGTTGCTCTCATCTTGTCAAAATTAGTTCAGGAAGCTTCTGTGACTGAGGTCTGTGAAGCCTTTAAAGTGGCTAGAGGTATGGTTCAAGCGCTACAGGAGAACGCTGGAAGGTTTTCTTCCATGGTTTCGGTATTCTGTGAGAGGCTTGGATGGCATGATTTGGAAGGATTAGTTGCCAAGTTCCAGAACCGTGTTTCATTTGGTGTCAGGGCTGAGATTGTTGAACTTACTAGCATTCCATATATAAAGGGTTCAAGAGCTAGAGCGCTGTATAAAGCTGGCTTGCGTACATCTCAGGCGATAGCTGAAGCGTCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCAGCGTGGGCTGCAGAAGGTACTGGACAAAGAAGGATACACTTGGGATTAGCCAAGAAAATCAAGAATGGGGCTCGCAAAATTGTTCTTGAGAAAGCAGAAGAGGCGAGGGCTGCTGCATTTTCTGCTTTTAAGTCACTTGGTTTGGATGTTAATGAGCTTTCAAAGCCCTTGCCGTTGGCTCCTGCTAGTAGTCTCAATGGACAAGAAACTACTGAAAGAGACATTTCCAGAGGCTCTGTTGGTCCCGATGGATTACAACAAAGCATAGAGGGGCACATGGAATGTGAAAACTTTGATATGGATAATCACAGAGAAAAGCCAAGTGAAGTATTAGGTGATGCTACCTTAGGGGTTTCATCAGAAATTAACTTGACCAGTCGTCTTCCTAATTTTAGACCTATAGGAACTGCAGTCGGCACTAATGGGCCTAGTGCTGTTAGTATTCTTTCCAGTGATACTTTCCCTATCCCTGTTTACGATAACAGAGAGATAAAACCTAAGGACAATGTTGAGCAGCATTTGACTAGGAATGACCATATTCCTCTGAGTAGTAATAAGGATGGTACAGGTGAAAAGGGTCCAGTAACTGCAGGAAATATTAGTGGCGGATTTGATTCTTTCTTGGAACTTTGGGGATCTGCTGGCGAATTTTTCTTCGATCTTCACTATAATAAACTACAAGATTTGAATTCACGCATCTCTTATGAGATACATGGAATTGCTATCTGCTGGAATTGCTCCCCTGTATACTATGTCAATCTTAATAAGGATCTTCCCAACCTGGAATGTGTAGAAAAACAAAAGCTTATCGAGGATGCAGTTATTGGCAAGAGTGAGGTTCTGGCTTCTCACAACATGTTGGATGTTATAAAGTCTAGGTGGAACAAAATCAGTAAAATTATGGGGAATGTAAATACGAGAAAGTTCACGTGGAATTTGAAAGTCCAGATTCAGGTGCTTAAGAGTCCCGCCATTTCAATCCAAAGATGCACTCGCTTGAATCTTCCAGAAGGGATCAGGGATGAGTTGGTCGATGGATCTTGGTTAATGATGCCTCCTCTTCACACAAGCCACACCATTGACATGAGCATTGTGATATGGATTCTTTGGCCAGATGAGGAAAGACACTCAAATCCAAACATCGATAAGGAAGTAAAGAAAAGGCTATCCCCAGAGGCAGCTGAAGCTGCTAATCGCAGTGGCAGGTGGAGGAATCAGATCCGAAGGGTTGCCCACAATGGTTGCTGCAGAAGGGTTGCTCAAACGAGAGCCCTTTGTTCAGCACTTTGGAAGATCCTTGTTTCCGAAGAACTTCTTCAAGCACTGACAACCATTGAAATGCCGCTGGTTAATGTCCTTGCAGACATGGAGCTTTGGGGTATAGGTATCGATATAGAAGGATGCCTTAGGGCACGGAATATATTGCGGGATAAACTGCGGTCCCTTGAGAAGAAAGCATTTGAATTGGCTGGCATGACATTTTCATTGCACAACCCAGCTGATATTGCAAATGTGTTGTTTGGACAGTTGAAGTTGCCCATACCTGAAAACCAATCTAAAGGGAAACTTCATCCAAGTACTGATAAGCATTGCTTGGATCTTTTAAGGAATGAGCATCCTGTTGTCCCAATTATCAAAGAACACCGCACTTTGGCAAAGCTCTTAAATTGTACACTTGGATCAATTTGTTCGCTTGCTAAACTACGCTTGAGCACGCAAAGGTACACTTTGCATGGCCGCTGGCTTCAAACATCAACAGCAACTGGGCGGCTTTCCATAGAAGAGCCTAATCTCCAATCTGTTGAGCATGAAGTAGAGTTCAAATTGGATAAAAATGGAAGGGACGTAAGTTCAGATGCTGATCGTTACAAAATAAATGCACGTGATTTCTTTGTTCCTACTCAGGAAAATTGGTTACTCTTGACGGCAGATTATTCACAGATAGAGTTGCGACTTATGGCACACTTTTCTAGAGATTCCTCGTTGATTTCAAAGCTTAGTCAGCCAGAAGGCGATGTCTTTACGATGATAGCTGCAAAATGGACTGGAAAAGCCGAGGATTCTGTTAGTCCACATGATAGAGATCAGACCAAAAGATTGATCTACGGAATTCTTTATGGAATGGGTGCAAACAGACTCGCAGAACAGCTTGAGTGCACCTCAGATGAAGCTAAGGAGAAAATTCGAAGCTTTAAAAGTTCTTTCCCTGCAGTCACCTCTTGGCTAAACGAAACGATTTCATTCTGCCAGGAAAAAGGATACATTCAGACTCTCAAGGGAAGAAGGCGGTTCTTGTCGAAAATAAAATTTGGGAATGCCAAAGAGAAATCGAAGGCTCAAAGGCAAGCTGTAAATTCCATGTGCCAGGGATCTGCTGCTGATATTATCAAGATTGCGATGATAAACATATATTCTGCAATTGCTGAAGATGTTGATACCGCAGCATCTAGTTCTTCAAGTGAAACTAGATTTCACATGTTGAAGGGACGATGCCGAATTCTTCTACAAGTACATGATGAACTTGTGCTGGAAGTTGATCCTTCTTACGTAAAACTAGCTGCAATGTTGCTACAAACCAGCATGGAAAATGCGGTGTCACTTCTTGTCCCTCTTCATGTGAAACTGAAAGTTGGGAAAACATGGGGTTCTTTAGAGCCGTTTCAGACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2154

Amino Acids

238.52

Weight (kDa)

8.15

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 527 - 701 1.3e-18 DEAD/DEAH box helicase
Helicase_C PF00271 833 - 908 4e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 913 - 1004 5.8e-26 Helix-turn-helix domain
POLQ_helical PF21099 1028 - 1212 9.2e-50 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1218 - 1289 5.1e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1736 - 2146 2.2e-112 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4661
AarI CACCTGC 2 cut(s) 2864, 4986
Acc16I TGCGCA 1 cut(s) 3293
Acc36I ACCTGC 5 cut(s) 1546, 2631, 2810, 2864, 4986
AccB1I GGYRCC 1 cut(s) 751
AccB7I CCANNNNNTGG 2 cut(s) 2105, 2660
AccI GTMKAC 3 cut(s) 1518, 3162, 4539
AccII CGCG 1 cut(s) 25
AclI AACGTT 1 cut(s) 2397
AcoI YGGCCR 3 cut(s) 2662, 4901, 5527
AcuI CTGAAG 5 cut(s) 1553, 3768, 3774, 4995, 6237
AcvI CACGTG 3 cut(s) 3125, 4722, 5675
AcyI GRCGYC 1 cut(s) 752
AdeI CACNNNGTG 2 cut(s) 863, 3305
AfeI AGCGCT 2 cut(s) 3531, 3708
AfiI CCNNNNNNNGG 9 cut(s) 195, 833, 1945, 2063, 2105, 2454, 2660, 4744, 5443
AflII CTTAAG 1 cut(s) 4751
AflIII ACRYGT 4 cut(s) 958, 2581, 4647, 6267
AhdI GACNNNNNGTC 1 cut(s) 4170
AhlI ACTAGT 2 cut(s) 440, 3133
AjiI CACGTC 1 cut(s) 3056
AjnI CCWGG 8 cut(s) 127, 790, 859, 2166, 2761, 4572, 6039, 6156
AleI CACNNNNGTG 1 cut(s) 1823
Alw21I GWGCWC 3 cut(s) 3124, 5507, 5951
Alw26I GTCTC 9 cut(s) 126, 423, 1487, 2463, 2549, 3017, 3240, 3989, 4005
Alw44I GTGCAC 1 cut(s) 5947
AlwNI CAGNNNCTG 4 cut(s) 2117, 4379, 5555, 5651
Ama87I CYCGRG 2 cut(s) 623, 2329
Aor51HI AGCGCT 2 cut(s) 3531, 3708
AoxI GGCC 6 cut(s) 325, 2065, 2662, 4217, 4901, 5527
ApaLI GTGCAC 1 cut(s) 5947
ArsI GACNNNNNNTTYG 4 cut(s) 65, 97, 2689, 2721
AseI ATTAAT 2 cut(s) 1164, 3180
Asp700I GAANNNNTTC 6 cut(s) 529, 885, 2493, 3553, 5103, 5106
AspLEI GCGC 6 cut(s) 754, 1662, 2111, 3294, 3532, 3709
AspS9I GGNCC 7 cut(s) 325, 1744, 2266, 4034, 4217, 4370, 5232
AsuC2I CCSGG 2 cut(s) 1664, 2264
AsuII TTCGAA 1 cut(s) 5977
AvaI CYCGRG 2 cut(s) 623, 2329
AvaII GGWCC 5 cut(s) 1744, 2266, 4034, 4370, 5232
AxyI CCTNAGG 3 cut(s) 4138, 4290, 5198
BaeGI GKGCMC 3 cut(s) 4068, 5206, 5951
BalI TGGCCA 1 cut(s) 4903
BanI GGYRCC 1 cut(s) 751
BanII GRGCYC 2 cut(s) 3865, 5065
BarI GAAGNNNNNNTAC 2 cut(s) 1104, 1136
BbrPI CACGTG 3 cut(s) 3125, 4722, 5675
BbsI GAAGAC 2 cut(s) 1559, 4166
Bbv12I GWGCWC 3 cut(s) 3124, 5507, 5951
BceAI ACGGC 4 cut(s) 2649, 3949, 5733, 6433
BciT130I CCWGG 8 cut(s) 129, 792, 861, 2168, 2763, 4574, 6041, 6158
BciVI GTATCC 4 cut(s) 1335, 1720, 3819, 6044
BclI TGATCA 2 cut(s) 31, 1198
BcnI CCSGG 2 cut(s) 1664, 2264
BcoDI GTCTC 9 cut(s) 126, 423, 1487, 2463, 2549, 3017, 3240, 3989, 4005
BcuI ACTAGT 2 cut(s) 440, 3133
BfmI CTRYAG 9 cut(s) 381, 933, 3533, 3801, 4191, 4200, 4380, 5038, 6000
BfoI RGCGCY 3 cut(s) 755, 3533, 3710
BfrI CTTAAG 1 cut(s) 4751
BfuAI ACCTGC 5 cut(s) 1546, 2631, 2810, 2864, 4986
BfuI GTATCC 4 cut(s) 1335, 1720, 3819, 6044
BglI GCCNNNNNGGC 1 cut(s) 1727
BglII AGATCT 3 cut(s) 122, 1379, 2432
BmcAI AGTACT 2 cut(s) 184, 5371
Bme18I GGWCC 5 cut(s) 1744, 2266, 4034, 4370, 5232
BmeRI GACNNNNNGTC 1 cut(s) 4170
BmeT110I CYCGRG 2 cut(s) 623, 2329
BmgBI CACGTC 1 cut(s) 3056
BmgT120I GGNCC 7 cut(s) 325, 1744, 2266, 4034, 4217, 4370, 5232
BmiI GGNNCC 6 cut(s) 753, 823, 3972, 4036, 4371, 5234
BmrI ACTGGG 3 cut(s) 2730, 3211, 5565
BmuI ACTGGG 3 cut(s) 2730, 3211, 5565
BoxI GACNNNNGTC 1 cut(s) 3488
BpiI GAAGAC 2 cut(s) 1559, 4166
BplI GAGNNNNNCTC 2 cut(s) 5936, 5968
BpmI CTGGAG 4 cut(s) 150, 2189, 2487, 2784
Bpu10I CCTNAGC 3 cut(s) 1293, 2970, 5964
Bpu14I TTCGAA 1 cut(s) 5977
BpuMI CCSGG 2 cut(s) 1664, 2264
Bsa29I ATCGAT 2 cut(s) 4935, 5181
BsaAI YACGTR 5 cut(s) 2582, 3125, 4722, 5675, 6340
BsaBI GATNNNNATC 5 cut(s) 2347, 2904, 3153, 5652, 5874
BsaHI GRCGYC 1 cut(s) 752
BsaI GGTCTC 2 cut(s) 2463, 2549
BsaJI CCNNGG 6 cut(s) 1550, 2166, 3049, 3561, 5847, 6157
BsaXI ACNNNNNCTCC 2 cut(s) 2730, 2760
Bsc4I CCNNNNNNNGG 9 cut(s) 195, 833, 1945, 2063, 2105, 2454, 2660, 4744, 5443
Bse21I CCTNAGG 3 cut(s) 4138, 4290, 5198
Bse3DI GCAATG 6 cut(s) 712, 1688, 2810, 5276, 5380, 6360
Bse8I GATNNNNATC 5 cut(s) 2347, 2904, 3153, 5652, 5874
BseBI CCWGG 8 cut(s) 129, 792, 861, 2168, 2763, 4574, 6041, 6158
BseCI ATCGAT 2 cut(s) 4935, 5181
BseDI CCNNGG 6 cut(s) 1550, 2166, 3049, 3561, 5847, 6157
BseJI GATNNNNATC 5 cut(s) 2347, 2904, 3153, 5652, 5874
BseLI CCNNNNNNNGG 9 cut(s) 195, 833, 1945, 2063, 2105, 2454, 2660, 4744, 5443
BseMI GCAATG 6 cut(s) 712, 1688, 2810, 5276, 5380, 6360
BseRI GAGGAG 4 cut(s) 1030, 2186, 3101, 4839
BseSI GKGCMC 3 cut(s) 4068, 5206, 5951
BseYI CCCAGC 3 cut(s) 2612, 2711, 5287
BsgI GTGCAG 2 cut(s) 491, 1819
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 6 cut(s) 327, 2067, 2664, 4219, 4903, 5529
BshNI GGYRCC 1 cut(s) 751
BshVI ATCGAT 2 cut(s) 4935, 5181
BsiHKAI GWGCWC 3 cut(s) 3124, 5507, 5951
BsiHKCI CYCGRG 2 cut(s) 623, 2329
BsiSI CCGG 2 cut(s) 1663, 2264
BslFI GGGAC 8 cut(s) 2047, 4020, 4743, 5218, 5402, 5648, 6291, 6383
BslI CCNNNNNNNGG 9 cut(s) 195, 833, 1945, 2063, 2105, 2454, 2660, 4744, 5443
BsmAI GTCTC 9 cut(s) 126, 423, 1487, 2463, 2549, 3017, 3240, 3989, 4005
BsmFI GGGAC 8 cut(s) 2047, 4020, 4743, 5218, 5402, 5648, 6291, 6383
BsmI GAATGC 2 cut(s) 3675, 6112
BsnI GGCC 6 cut(s) 327, 2067, 2664, 4219, 4903, 5529
Bso31I GGTCTC 2 cut(s) 2463, 2549
BsoBI CYCGRG 2 cut(s) 623, 2329
Bsp119I TTCGAA 1 cut(s) 5977
Bsp1286I GDGCHC 7 cut(s) 3124, 3865, 4068, 5065, 5206, 5507, 5951
Bsp1407I TGTACA 2 cut(s) 2921, 5462
Bsp19I CCATGG 1 cut(s) 3561
BspANI GGCC 6 cut(s) 327, 2067, 2664, 4219, 4903, 5529
BspDI ATCGAT 2 cut(s) 4935, 5181
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 6 cut(s) 753, 823, 3972, 4036, 4371, 5234
BspMAI CTGCAG 5 cut(s) 3805, 4204, 4384, 5042, 6004
BspMI ACCTGC 5 cut(s) 1546, 2631, 2810, 2864, 4986
BspQI GCTCTTC 1 cut(s) 5568
BspT104I TTCGAA 1 cut(s) 5977
BspT107I GGYRCC 1 cut(s) 751
BspTI CTTAAG 1 cut(s) 4751
BspTNI GGTCTC 2 cut(s) 2463, 2549
BsrDI GCAATG 6 cut(s) 712, 1688, 2810, 5276, 5380, 6360
BsrGI TGTACA 2 cut(s) 2921, 5462
BssECI CCNNGG 6 cut(s) 1550, 2166, 3049, 3561, 5847, 6157
BssNAI GTATAC 2 cut(s) 1519, 4540
BssNI GRCGYC 1 cut(s) 752
BssT1I CCWWGG 3 cut(s) 1550, 3049, 3561
Bst1107I GTATAC 2 cut(s) 1519, 4540
Bst2UI CCWGG 8 cut(s) 129, 792, 861, 2168, 2763, 4574, 6041, 6158
Bst6I CTCTTC 9 cut(s) 47, 1872, 2499, 2985, 3015, 3885, 4857, 5568, 6408
BstACI GRCGYC 1 cut(s) 752
BstAFI CTTAAG 1 cut(s) 4751
BstAPI GCANNNNNTGC 3 cut(s) 1289, 3305, 3914
BstAUI TGTACA 2 cut(s) 2921, 5462
BstBAI YACGTR 5 cut(s) 2582, 3125, 4722, 5675, 6340
BstBI TTCGAA 1 cut(s) 5977
BstDSI CCRYGG 1 cut(s) 3561
BstEII GGTNACC 1 cut(s) 1934
BstENI CCTNNNNNAGG 1 cut(s) 831
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 3 cut(s) 755, 3533, 3710
BstHHI GCGC 6 cut(s) 754, 1662, 2111, 3294, 3532, 3709
BstMAI GTCTC 9 cut(s) 126, 423, 1487, 2463, 2549, 3017, 3240, 3989, 4005
BstNI CCWGG 8 cut(s) 129, 792, 861, 2168, 2763, 4574, 6041, 6158
BstNSI RCATGY 4 cut(s) 962, 1772, 4651, 6271
BstPAI GACNNNNGTC 1 cut(s) 3488
BstPI GGTNACC 1 cut(s) 1934
BstSFI CTRYAG 9 cut(s) 381, 933, 3533, 3801, 4191, 4200, 4380, 5038, 6000
BstSLI GKGCMC 3 cut(s) 4068, 5206, 5951
BstSNI TACGTA 1 cut(s) 6340
BstUI CGCG 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 1559, 4166
BstXI CCANNNNNNTGG 1 cut(s) 791
BstZ17I GTATAC 2 cut(s) 1519, 4540
Bsu15I ATCGAT 2 cut(s) 4935, 5181
Bsu36I CCTNAGG 3 cut(s) 4138, 4290, 5198
BsuI GTATCC 4 cut(s) 1335, 1720, 3819, 6044
BsuRI GGCC 6 cut(s) 327, 2067, 2664, 4219, 4903, 5529
BsuTUI ATCGAT 2 cut(s) 4935, 5181
BtgI CCRYGG 1 cut(s) 3561
BtgZI GCGATG 4 cut(s) 1198, 2891, 5820, 6203
BtrI CACGTC 1 cut(s) 3056
BtsI GCAGTG 3 cut(s) 1519, 2047, 4996
BtsIMutI CAGTG 7 cut(s) 999, 1519, 2047, 2319, 4249, 4996, 5114
BveI ACCTGC 5 cut(s) 1546, 2631, 2810, 2864, 4986
CaiI CAGNNNCTG 4 cut(s) 2117, 4379, 5555, 5651
CfoI GCGC 6 cut(s) 754, 1662, 2111, 3294, 3532, 3709
Cfr13I GGNCC 7 cut(s) 325, 1744, 2266, 4034, 4217, 4370, 5232
ClaI ATCGAT 2 cut(s) 4935, 5181
CseI GACGC 2 cut(s) 509, 3741
CsiI ACCWGGT 1 cut(s) 859
CspCI CAANNNNNGTGG 4 cut(s) 1059, 1094, 5015, 5050
DinI GGCGCC 1 cut(s) 753
DraI TTTAAA 3 cut(s) 78, 3505, 5986
DraIII CACNNNGTG 2 cut(s) 863, 3305
DriI GACNNNNNGTC 1 cut(s) 4170
EaeI YGGCCR 3 cut(s) 2662, 4901, 5527
Eam1104I CTCTTC 9 cut(s) 47, 1872, 2499, 2985, 3015, 3885, 4857, 5568, 6408
Eam1105I GACNNNNNGTC 1 cut(s) 4170
EarI CTCTTC 9 cut(s) 47, 1872, 2499, 2985, 3015, 3885, 4857, 5568, 6408
EciI GGCGGA 2 cut(s) 1745, 4413
Eco105I TACGTA 1 cut(s) 6340
Eco130I CCWWGG 3 cut(s) 1550, 3049, 3561
Eco24I GRGCYC 2 cut(s) 3865, 5065
Eco31I GGTCTC 2 cut(s) 2463, 2549
Eco32I GATATC 1 cut(s) 2428
Eco47I GGWCC 5 cut(s) 1744, 2266, 4034, 4370, 5232
Eco47III AGCGCT 2 cut(s) 3531, 3708
Eco57I CTGAAG 5 cut(s) 1553, 3768, 3774, 4995, 6237
Eco72I CACGTG 3 cut(s) 3125, 4722, 5675
Eco81I CCTNAGG 3 cut(s) 4138, 4290, 5198
Eco88I CYCGRG 2 cut(s) 623, 2329
Eco91I GGTNACC 1 cut(s) 1934
EcoNI CCTNNNNNAGG 1 cut(s) 831
EcoO109I RGGNCCY 1 cut(s) 1744
EcoO65I GGTNACC 1 cut(s) 1934
EcoRI GAATTC 5 cut(s) 779, 2932, 4480, 5901, 6288
EcoRII CCWGG 8 cut(s) 127, 790, 859, 2166, 2761, 4572, 6039, 6156
EcoRV GATATC 1 cut(s) 2428
EcoT14I CCWWGG 3 cut(s) 1550, 3049, 3561
EcoT38I GRGCYC 2 cut(s) 3865, 5065
EgeI GGCGCC 1 cut(s) 753
EheI GGCGCC 1 cut(s) 753
ErhI CCWWGG 3 cut(s) 1550, 3049, 3561
FaqI GGGAC 8 cut(s) 2047, 4020, 4743, 5218, 5402, 5648, 6291, 6383
FauI CCCGC 2 cut(s) 4768, 5211
FauNDI CATATG 1 cut(s) 1926
FbaI TGATCA 2 cut(s) 31, 1198
FblI GTMKAC 3 cut(s) 1518, 3162, 4539
FriOI GRGCYC 2 cut(s) 3865, 5065
FspAI RTGCGCAY 1 cut(s) 3293
FspI TGCGCA 1 cut(s) 3293
GlaI GCGC 6 cut(s) 753, 1661, 2110, 3293, 3531, 3708
GsaI CCCAGC 3 cut(s) 2616, 2715, 5291
GsuI CTGGAG 4 cut(s) 150, 2189, 2487, 2784
HaeII RGCGCY 3 cut(s) 755, 3533, 3710
HaeIII GGCC 6 cut(s) 327, 2067, 2664, 4219, 4903, 5529
HapII CCGG 2 cut(s) 1663, 2264
HgaI GACGC 2 cut(s) 509, 3741
HhaI GCGC 6 cut(s) 754, 1662, 2111, 3294, 3532, 3709
Hin1I GRCGYC 1 cut(s) 752
Hin6I GCGC 6 cut(s) 752, 1660, 2109, 3292, 3530, 3707
HinP1I GCGC 6 cut(s) 752, 1660, 2109, 3292, 3530, 3707
HincII GTYRAC 2 cut(s) 1971, 2620
HindII GTYRAC 2 cut(s) 1971, 2620
HindIII AAGCTT 4 cut(s) 3474, 4595, 5786, 5980
HpaI GTTAAC 1 cut(s) 2620
HpaII CCGG 2 cut(s) 1663, 2264
Hpy99I CGWCG 2 cut(s) 525, 1253
HpyCH4IV ACGT 8 cut(s) 2397, 2581, 3055, 3124, 4721, 5637, 5674, 6339
HpySE526I ACGT 8 cut(s) 2397, 2581, 3055, 3124, 4721, 5637, 5674, 6339
Hsp92I GRCGYC 1 cut(s) 752
HspAI GCGC 6 cut(s) 752, 1660, 2109, 3292, 3530, 3707
KasI GGCGCC 1 cut(s) 751
Ksp22I TGATCA 2 cut(s) 31, 1198
KspAI GTTAAC 1 cut(s) 2620
LguI GCTCTTC 1 cut(s) 5568
LmnI GCTCC 3 cut(s) 3976, 4535, 5161
MabI ACCWGGT 1 cut(s) 859
MaeII ACGT 8 cut(s) 2397, 2581, 3055, 3124, 4721, 5637, 5674, 6339
MfeI CAATTG 2 cut(s) 915, 6210
MhlI GDGCHC 7 cut(s) 3124, 3865, 4068, 5065, 5206, 5507, 5951
MlsI TGGCCA 1 cut(s) 4903
MluNI TGGCCA 1 cut(s) 4903
Mly113I GGCGCC 1 cut(s) 752
MlyI GAGTC 8 cut(s) 590, 1489, 2341, 2842, 3098, 4765, 5922, 6055
MmeI TCCRAC 3 cut(s) 799, 3247, 4632
Mox20I TGGCCA 1 cut(s) 4903
MroXI GAANNNNTTC 6 cut(s) 529, 885, 2493, 3553, 5103, 5106
MscI TGGCCA 1 cut(s) 4903
MslI CAYNNNNRTG 5 cut(s) 1823, 2910, 3395, 4874, 5129
Msp20I TGGCCA 1 cut(s) 4903
MspA1I CMGCKG 5 cut(s) 368, 4973, 5137, 5291, 5532
MspCI CTTAAG 1 cut(s) 4751
MspI CCGG 2 cut(s) 1663, 2264
MunI CAATTG 2 cut(s) 915, 6210
Mva1269I GAATGC 2 cut(s) 3675, 6112
MvaI CCWGG 8 cut(s) 129, 792, 861, 2168, 2763, 4574, 6041, 6158
MvnI CGCG 1 cut(s) 25
NarI GGCGCC 1 cut(s) 752
NciI CCSGG 2 cut(s) 1664, 2264
NcoI CCATGG 1 cut(s) 3561
NdeI CATATG 1 cut(s) 1926
NlaIV GGNNCC 6 cut(s) 753, 823, 3972, 4036, 4371, 5234
NmeAIII GCCGAG 3 cut(s) 726, 1747, 5872
NmuCI GTSAC 9 cut(s) 66, 109, 1934, 3090, 3169, 3481, 3924, 6004, 6387
NsbI TGCGCA 1 cut(s) 3293
NspI RCATGY 4 cut(s) 962, 1772, 4651, 6271
NspV TTCGAA 1 cut(s) 5977
OliI CACNNNNGTG 1 cut(s) 1823
PaeR7I CTCGAG 1 cut(s) 2329
PaqCI CACCTGC 2 cut(s) 2864, 4986
PciI ACATGT 3 cut(s) 958, 4647, 6267
PciSI GCTCTTC 1 cut(s) 5568
PcsI WCGNNNNNNNCGW 1 cut(s) 2713
PctI GAATGC 2 cut(s) 3675, 6112
PdmI GAANNNNTTC 6 cut(s) 529, 885, 2493, 3553, 5103, 5106
PflMI CCANNNNNTGG 2 cut(s) 2105, 2660
PfoI TCCNGGA 1 cut(s) 127
PleI GAGTC 8 cut(s) 589, 1488, 2340, 2842, 3097, 4764, 5922, 6055
PluTI GGCGCC 1 cut(s) 755
PmaCI CACGTG 3 cut(s) 3125, 4722, 5675
PmlI CACGTG 3 cut(s) 3125, 4722, 5675
PpsI GAGTC 8 cut(s) 589, 1488, 2340, 2842, 3097, 4764, 5922, 6055
Ppu21I YACGTR 5 cut(s) 2582, 3125, 4722, 5675, 6340
PpuMI RGGWCCY 1 cut(s) 1744
PscI ACATGT 3 cut(s) 958, 4647, 6267
PshAI GACNNNNGTC 1 cut(s) 3488
PshBI ATTAAT 2 cut(s) 1164, 3180
PsiI TTATAA 1 cut(s) 4661
Psp1406I AACGTT 1 cut(s) 2397
Psp5II RGGWCCY 1 cut(s) 1744
Psp6I CCWGG 8 cut(s) 127, 790, 859, 2166, 2761, 4572, 6039, 6156
PspCI CACGTG 3 cut(s) 3125, 4722, 5675
PspEI GGTNACC 1 cut(s) 1934
PspFI CCCAGC 3 cut(s) 2612, 2711, 5287
PspGI CCWGG 8 cut(s) 127, 790, 859, 2166, 2761, 4572, 6039, 6156
PspN4I GGNNCC 6 cut(s) 753, 823, 3972, 4036, 4371, 5234
PspPI GGNCC 7 cut(s) 325, 1744, 2266, 4034, 4217, 4370, 5232
PspPPI RGGWCCY 1 cut(s) 1744
PspXI VCTCGAGB 1 cut(s) 2329
PstI CTGCAG 5 cut(s) 3805, 4204, 4384, 5042, 6004
PstNI CAGNNNCTG 4 cut(s) 2117, 4379, 5555, 5651
PvuII CAGCTG 3 cut(s) 368, 4973, 5291
RseI CAYNNNNRTG 5 cut(s) 1823, 2910, 3395, 4874, 5129
SapI GCTCTTC 1 cut(s) 5568
Sau96I GGNCC 7 cut(s) 325, 1744, 2266, 4034, 4217, 4370, 5232
ScaI AGTACT 2 cut(s) 184, 5371
SchI GAGTC 8 cut(s) 590, 1489, 2341, 2842, 3098, 4765, 5922, 6055
SduI GDGCHC 7 cut(s) 3124, 3865, 4068, 5065, 5206, 5507, 5951
SexAI ACCWGGT 1 cut(s) 859
SfcI CTRYAG 9 cut(s) 381, 933, 3533, 3801, 4191, 4200, 4380, 5038, 6000
SfoI GGCGCC 1 cut(s) 753
Sfr274I CTCGAG 1 cut(s) 2329
SfuI TTCGAA 1 cut(s) 5977
SinI GGWCC 5 cut(s) 1744, 2266, 4034, 4370, 5232
SlaI CTCGAG 1 cut(s) 2329
SmiMI CAYNNNNRTG 5 cut(s) 1823, 2910, 3395, 4874, 5129
SnaBI TACGTA 1 cut(s) 6340
SpeI ACTAGT 2 cut(s) 440, 3133
SspDI GGCGCC 1 cut(s) 751
SspI AATATT 1 cut(s) 4390
StyI CCWWGG 3 cut(s) 1550, 3049, 3561
TaiI ACGT 8 cut(s) 2400, 2584, 3058, 3127, 4724, 5640, 5677, 6342
TatI WGTACW 7 cut(s) 182, 1696, 2479, 2921, 5369, 5462, 6301
TauI GCSGC 4 cut(s) 2612, 5137, 5532, 5563
TscAI CASTG 7 cut(s) 1006, 1519, 2047, 2319, 4249, 4996, 5121
TseFI GTSAC 9 cut(s) 66, 109, 1934, 3090, 3169, 3481, 3924, 6004, 6387
Tsp45I GTSAC 9 cut(s) 66, 109, 1934, 3090, 3169, 3481, 3924, 6004, 6387
TspGWI ACGGA 4 cut(s) 348, 2451, 5221, 5913
TspRI CASTG 7 cut(s) 1006, 1519, 2047, 2319, 4249, 4996, 5121
Van91I CCANNNNNTGG 2 cut(s) 2105, 2660
Vha464I CTTAAG 1 cut(s) 4751
VneI GTGCAC 1 cut(s) 5947
VpaK11BI GGWCC 5 cut(s) 1744, 2266, 4034, 4370, 5232
VspI ATTAAT 2 cut(s) 1164, 3180
XagI CCTNNNNNAGG 1 cut(s) 831
XbaI TCTAGA 1 cut(s) 5762
XceI RCATGY 4 cut(s) 962, 1772, 4651, 6271
XhoI CTCGAG 1 cut(s) 2329
XmiI GTMKAC 3 cut(s) 1518, 3162, 4539
XmnI GAANNNNTTC 6 cut(s) 529, 885, 2493, 3553, 5103, 5106
ZrmI AGTACT 2 cut(s) 184, 5371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.