Rw6G039610

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013097
Physical Location & Seq
Forward (+)
63576318 .. 63589613
13296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039610.1

Sequence Viewer

Length: 6309 bp
ATGGCGTCTGGTTCGCCTCCTCCGTCGCGCATCAATCAGTTTTTTGCTTCGAAGAAACGGAAGCCTGTATCACCTGCTTTGAAGTCGGGGAGAAATGAAAAGGATGTACAAACTGTAGAAGGTTCACCTAGTGCTAAGGCCACATTGGACAGTTATTTGCGGACATCGCCGGAGAGTTATAATGTGGCGAAACCTTTGTGCAAATTTGGTGATTCAGCGGCTCGGCAAGATCAAGTTAGAAGGAATTTGGAATCTGAGATGGATAATTCTTCAAAAAATGAATGTATGCAGCACCGTTTCTCTTCTCAACTGCACACTCATGCAAGTGAGGCTTCTGGAGCAAATCAGAAGGAAATAGCTAAGGGATTAACCGAGGCGGGTGATGTTGTAGCTGGAGGTTCTTCCAAGGATTACTCAGATTTTGCAGGAGGTGTGGAGAATATAGAGCTGAAGAAGTTTGCAGCCAACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAGTTCTGTGTCAGAGCTGAAAGTGAATGGTCATAAGAGATACCCTAGCCCATCTTTGCTTGATGGGGAGAGTAAGACATTTAAAAAGAGGCATTGCGGTAGTGATAAATCCTATGAAGAGCTTACCATTCCTAATGAATTGCTCGAGCTTCAACAAACCTTGAAAACATGCAGTAATACACCCAAACTGTCTCTAGACGTAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAATACGTGGTAGCTCATTTTCTTCACCTGGTGAAGCTTTTTGGAACGATGCAATTCAACTTGCTGATGGTCTATGTGCCCAGGCAGCAGAAGCAACTACAGCTTCAGATAGCCAATATCATGGTATAAACTCTTGCAATTTGAGAAATGCACACTGTGATGAAAAGTCAAAGGAAATGATGGATGAAGGTGAGCGTATGGGTAATGGTGCTGTATCAGAGCCAATGGTAAAGCATAGGAAGGACTTGCATAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGATTTCTCTCTTGAGGACAAGATTTCGAATGAAAGTATGCCACATCATATAGATTCTTACAATTTGAAAAGCACGGCTCATGGAGGTGGCGAGCAATCTGAATCCAGTCTTATAGATCCTAGAGGTCTAAGACATGGTATTACTATGGTCCGTTGCAGCAAATCTCAGGCAAATCAAGGGCAAGATGTTGATTCTGTTACTGCAATGACCAAAATGAAAGTGGATTTGTCTAGCAAGGATAATGGTGGCCCGACATTTTCTTCACCAGGTGATGATGTCGTTAAGTTGAATGGTAAAGGTGAATCTAGCGAAGAAAGTACTCCTTCAAGCTTTCTGCCACTGAAGGATTGTCTGGATCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCCAAGCTTTATCCTTGGCAGGTTGAATGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACGAGTGCAGGTAAAAGTTTCGTTGCAGAAATTCTGATGCTGCGGCAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCTGAACATCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGGAACCAAGGTGCTGGTACACTTCCTAAAGACACTACTGTAGCTGTCTGCACAATAGAAAAGGCAAACTTTTTAGTAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCTGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGACCCTCCCATGGTTTGCAAATTGTTGGCTGCCCTTTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATTAAAGTGGGGAACACCATTTACAGCAAGAAAATGGAGATTATTAGAACAATCCCAAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCATGTTGTGGAACTGTGCAATGAGGTTGTACAAGAAGGTCTTTCCGTGTTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTGCAGTCAACATTCGCACTGATGACAGTGAATTTAATGATGTGACATTGGCTATTGATTCCTTACGAAGGTGTCCTGCTGGTTTGGATCCAGTGTTAGAAGAAACCCTACCTTCTGGAGTTGCCTACCACCATGCTGGGCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAAGGCCATGTACGCGTCTTAACTGCAACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGAGTCATTTTTCGTCAGCCCCGGATTGGTCGCGATTTTATCGATGGGACAAGGTACAGGCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTAATTTGCAAGCCAGAAGAGATCAAAAGGATTATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTTGTAGCAGGGGGAATTGTTCAAACTGCTAATGACATTCATCGCTATGTTAGGTGCACACTTTTAAACTCCACTAAACCTTTTCAGGATGTGGTGAAATCAGCACAGGAATCTCTCCGTTGGTTGTGCCACAAGAAATTTCTAGAATGGAATGACGACACTAAGTTATACAATACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTATGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGCTTTGTGCTTGCTTCTGATTTGCATCTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGAACAGTTCATGGAATTGTCTGCTCTTGACCAGTCCGTAGGCAATCGCGTTGGAGTGACAGAACCATTTTTGATGCGAATGGCACATGGTGCACCTATGCGGTCATCAAACAGGATGAGAGAAAATACGAAAGCATTGCATGGCAATCATGAAAGCCGACTTGGGACTACAAAGAGTACTGTGCTTCCGGATGATCAAACAGTTCGAGTTTGTAAACGGTTCTATGTTGCTCTGATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTCTGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGCCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACTATGTTTTGTGAAAGGCTCGGATGGCATGATCTGGAAGGCTTGGTTGGAAAATTTCAAAACCGTGTTTCATTTGGAGTGAGAGCAGAGATTGTAGAGCTGACTGCAATTCCATTTGTCAAGGGTTCTCGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACGCCTTTAGCTATTGCTGAAGCATCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCATCGTGGGCAGCACAAGAAGGTTCAGCTCAAAGACGCATCCAAGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATTGTTCTTGAAAAGGCGGAAGAGGCCAGAGTTGCTGCCTTCTCTGCTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCGACCATTATTTCCAACTGCTGGTGGAAGTCCCACTATGCAAGGAACAACAAATTCCTCTGGTGATGAAAATACTACTGGCTTTGTTAATGCCCATGTGGAGGATGCTCTTAAGCCATCTTTGGAAGGAAGGGTTCCTTCTGGTAAGGTTGTATCAGGTGCAAAGTTGACAAAAACATCAGATATTGGTACGTTGGTGGCTTCAGGAGAAGTAGCTTCAATTGGTGTCATACAAATTAAGTTTGGGGCTGAAAATTCTGTAGTACCAATTGATGGGTCTGGTGCCCTTGCTGATGAGCTAAATGCTACTGTTCATCAAACTAAAATTGCTGACTTGACCAACTCGATTAAGGTAGACAGCTTAGGTGATAGAAATAGGATATCCAGCAAGTCTTTAGATCCTAAAAAGCAGGGAAGCTGTGAAGGGGAAAATTTGTCTGTTGGTGTTAAGGGCAATGCTTGTGAAAAAGGCCCTATTCATGCAATTTATACTCCTGGTGGAATCGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTTCATTTTGATATCCACTACAACAAAAGATCAGAATTGAACTCTGTTGCCCCCTTTGAAATTCATGGAATGGCCATCTGTTGGGAAGATTCTCCTGTGTACTATGTTAACATTCCTAAGGATCTGCTGTGGTCTGGCAGCAGCAGTAAAGACGAAGGGCTGCGTTTGACTGCATCAGGTGATAGAACTAATGTGTTACCTCTTGATGGTATGTTGGATCTGGCTAAACGTAGATGGAGCAGGATTGGTGAAATCATGGGAAAAGAGATTCAGGCATTGAAGTGCCCTGCAGTTCAAATACAGAGATTTGGTTCCCAGAGTCTTGCAGGGAAAAGTATTTGTTTTGAAATTATAGACACCTCGTTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTTTGGCCTGATGAGGAGAAAAATTCTAATCCAAACTTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGTGACGCTGCAGCAGCTGCAAATCGAAACGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCTCAAACACGGGCCTTATATTCTGTTCTGTGGAAGTTGCTTGTTTCTGAAGACCTTGCTGAGTCACTTATAAATATAGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGATATGGAGGGATGTATGCAAGCACGTAATGTGCTGGGGAAGAAGCTAAGACATCTTGAGAAAGAAGCTTATAAACTGGCTGGCATGACATTTTCATTATATACAGCAGCAGATATTGCTAATGTAGGTGTGCAACAAGGAAAACAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGACGAGCATCCTATCATTCCCATCATCAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGTTGGGATCAATTTGCTCACTTGCTAGGCTATCTGTGAAGACACAGAAGTACACATTACACGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGTATGAAAATGGAGGCAAAAGTGATGTTGATCATTACAAAATTAATGCTCGAGAATACTTTATACCTACTCAGGACAACTGGATACTGTTGACCGCAGATTATTCTCAGATAGAACTGCGGCTGATGGCCCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGTGATGTCTTCACCATGATTGCAGCTAGATGGACTGGAATGTCAGAAGATTGTATTAGTTCTGATGTACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTACGGAATGGGTGCTAATAGCCTTGCAGAACAACTGGATTGCAGTCCCCAGGCAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTCCCCGGAGTTGCTTCTTGGCTCAATGAAGCAGTTGGATGTTGCCGCAAGAAAGGATATGTGGAAACCCTTAAGGGAAGAAAACGATTTTTGTCAAAAATAAAATTTGGGAACAGCAAGGAAAAGTCAAAGGCGCAGAGACAGGCTGTAAATTCGATATGTCAGGGATCAGCTGCTGACATAATCAAGATTGCAATGATAAATATTTACTCCGTAATAGTTGATGGAGCTGAAAAGCCTGATTCGACTTCTCCATTTGTACATGATGAATTGGTACTTGAAGCTGATCCTTCTGTGATAAAGGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAGTGCTGTTTCGCTTCTTGTTCCTATGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCTGACCAATATGAAGATCTGCCTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2102

Amino Acids

231.01

Weight (kDa)

6.78

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 502 - 636 4.4e-16 DEAD/DEAH box helicase
Helicase_C PF00271 791 - 866 1.3e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 871 - 962 9.3e-25 Helix-turn-helix domain
POLQ_helical PF21099 986 - 1170 9.3e-53 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1176 - 1250 8.6e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1699 - 2086 5.6e-102 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 180, 5021, 5154
AarI CACCTGC 2 cut(s) 82, 4887
AasI GACNNNNNNGTC 1 cut(s) 5710
Acc36I ACCTGC 6 cut(s) 82, 1471, 1541, 1579, 2441, 4887
Acc65I GGTACC 1 cut(s) 2035
AccB1I GGYRCC 2 cut(s) 2035, 4112
AccB7I CCANNNNNTGG 4 cut(s) 3851, 4103, 4452, 5461
AccI GTMKAC 1 cut(s) 4185
AccII CGCG 4 cut(s) 28, 2457, 2541, 3138
AccIII TCCGGA 1 cut(s) 3277
AcoI YGGCCR 1 cut(s) 4443
AcuI CTGAAG 9 cut(s) 470, 855, 1412, 1478, 2724, 2979, 3642, 4017, 5019
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 6 cut(s) 131, 797, 923, 1319, 1643, 3179
AflII CTTAAG 3 cut(s) 2252, 3941, 5933
AflIII ACRYGT 2 cut(s) 2455, 4776
AgeI ACCGGT 1 cut(s) 2032
AhlI ACTAGT 1 cut(s) 3337
AjiI CACGTC 1 cut(s) 2930
AjnI CCWGG 8 cut(s) 724, 793, 846, 1315, 3399, 4324, 4790, 5823
AjuI GAANNNNNNNTTGG 4 cut(s) 3234, 3266, 3471, 3503
AloI GAACNNNNNNTCC 2 cut(s) 5436, 5468
Alw21I GWGCWC 3 cut(s) 2798, 3184, 3742
Alw26I GTCTC 2 cut(s) 702, 5995
Alw44I GTGCAC 3 cut(s) 2794, 3180, 3738
AlwNI CAGNNNCTG 4 cut(s) 4880, 5423, 5834, 6038
Ama87I CYCGRG 3 cut(s) 650, 3570, 5541
Aor13HI TCCGGA 1 cut(s) 3277
ApaLI GTGCAC 3 cut(s) 2794, 3180, 3738
ArsI GACNNNNNNTTYG 2 cut(s) 2563, 2595
AseI ATTAAT 1 cut(s) 5535
AsiGI ACCGGT 1 cut(s) 2032
Asp700I GAANNNNTTC 2 cut(s) 1491, 4336
Asp718I GGTACC 1 cut(s) 2035
AspLEI GCGC 3 cut(s) 30, 2691, 5998
AspS9I GGNCC 8 cut(s) 1198, 1298, 1807, 3397, 3817, 4301, 4962, 5620
AsuC2I CCSGG 2 cut(s) 2530, 5868
AsuII TTCGAA 2 cut(s) 50, 1076
AvaI CYCGRG 3 cut(s) 650, 3570, 5541
AvaII GGWCC 3 cut(s) 1198, 3397, 3817
AxyI CCTNAGG 1 cut(s) 4488
BaeGI GKGCMC 7 cut(s) 847, 2798, 3184, 3583, 3742, 4117, 4658
BaeI ACNNNNGTAYC 2 cut(s) 4011, 4044
BalI TGGCCA 1 cut(s) 4445
BamHI GGATCC 1 cut(s) 2347
BanI GGYRCC 2 cut(s) 2035, 4112
BanII GRGCYC 1 cut(s) 2403
BarI GAAGNNNNNNTAC 2 cut(s) 2352, 2384
BbsI GAAGAC 3 cut(s) 5007, 5378, 5671
Bbv12I GWGCWC 3 cut(s) 2798, 3184, 3742
BceAI ACGGC 4 cut(s) 1140, 1794, 4909, 4926
BcgI CGANNNNNNTGC 2 cut(s) 3208, 3242
BciT130I CCWGG 8 cut(s) 726, 795, 848, 1317, 3401, 4326, 4792, 5825
BciVI GTATCC 2 cut(s) 1036, 5568
BclI TGATCA 2 cut(s) 3283, 5521
BcnI CCSGG 2 cut(s) 2530, 5868
BcoDI GTCTC 2 cut(s) 702, 5995
BcuI ACTAGT 1 cut(s) 3337
BfmI CTRYAG 6 cut(s) 114, 864, 1749, 4089, 4659, 4872
BfrI CTTAAG 3 cut(s) 2252, 3941, 5933
BfuAI ACCTGC 6 cut(s) 82, 1471, 1541, 1579, 2441, 4887
BfuI GTATCC 2 cut(s) 1036, 5568
BglII AGATCT 1 cut(s) 6292
BmcAI AGTACT 3 cut(s) 1369, 3268, 5239
Bme18I GGWCC 3 cut(s) 1198, 3397, 3817
BmeT110I CYCGRG 3 cut(s) 650, 3570, 5541
BmgBI CACGTC 1 cut(s) 2930
BmgT120I GGNCC 8 cut(s) 1198, 1298, 1807, 3397, 3817, 4301, 4962, 5620
BmiI GGNNCC 9 cut(s) 1715, 2037, 2349, 3819, 3966, 4114, 4684, 5405, 5445
BpiI GAAGAC 3 cut(s) 5007, 5378, 5671
BplI GAGNNNNNCTC 2 cut(s) 5624, 5656
BpmI CTGGAG 5 cut(s) 357, 414, 1685, 2397, 5438
Bpu10I CCTNAGC 4 cut(s) 135, 360, 2479, 4192
Bpu14I TTCGAA 2 cut(s) 50, 1076
BpuEI CTTGAG 2 cut(s) 1082, 5159
BpuMI CCSGG 2 cut(s) 2530, 5868
Bsa29I ATCGAT 2 cut(s) 2550, 4335
BsaAI YACGTR 2 cut(s) 773, 5108
BsaBI GATNNNNATC 4 cut(s) 2139, 2221, 3027, 5520
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 2 cut(s) 2032, 3277
BsaXI ACNNNNNCTCC 4 cut(s) 2604, 2634, 5436, 5466
Bse118I RCCGGY 1 cut(s) 2032
Bse21I CCTNAGG 1 cut(s) 4488
Bse3DI GCAATG 8 cut(s) 598, 1260, 2167, 2684, 3224, 4291, 5248, 6063
Bse8I GATNNNNATC 4 cut(s) 2139, 2221, 3027, 5520
BseAI TCCGGA 1 cut(s) 3277
BseBI CCWGG 8 cut(s) 726, 795, 848, 1317, 3401, 4326, 4792, 5825
BseCI ATCGAT 2 cut(s) 2550, 4335
BseJI GATNNNNATC 4 cut(s) 2139, 2221, 3027, 5520
BseMI GCAATG 8 cut(s) 598, 1260, 2167, 2684, 3224, 4291, 5248, 6063
BseMII CTCAG 9 cut(s) 246, 429, 1229, 4380, 5001, 5576, 5612, 5622, 5826
BseRI GAGGAG 2 cut(s) 9, 4826
BseSI GKGCMC 7 cut(s) 847, 2798, 3184, 3583, 3742, 4117, 4658
BseYI CCCAGC 2 cut(s) 2396, 5116
BsgI GTGCAG 7 cut(s) 296, 1569, 1665, 1744, 1832, 2952, 4908
Bsh1236I CGCG 4 cut(s) 28, 2457, 2541, 3138
Bsh1285I CGRYCG 1 cut(s) 2033
BshNI GGYRCC 2 cut(s) 2035, 4112
BshTI ACCGGT 1 cut(s) 2032
BshVI ATCGAT 2 cut(s) 2550, 4335
BsiEI CGRYCG 1 cut(s) 2033
BsiHKAI GWGCWC 3 cut(s) 2798, 3184, 3742
BsiHKCI CYCGRG 3 cut(s) 650, 3570, 5541
BsiSI CCGG 5 cut(s) 170, 2033, 2530, 3278, 5868
BsiWI CGTACG 1 cut(s) 3602
BslFI GGGAC 6 cut(s) 2569, 3268, 3803, 3846, 5282, 5805
BsmAI GTCTC 2 cut(s) 702, 5995
BsmFI GGGAC 6 cut(s) 2569, 3268, 3803, 3846, 5282, 5805
BsmI GAATGC 2 cut(s) 1493, 3421
BsoBI CYCGRG 3 cut(s) 650, 3570, 5541
Bsp119I TTCGAA 2 cut(s) 50, 1076
Bsp1286I GDGCHC 8 cut(s) 847, 2403, 2798, 3184, 3583, 3742, 4117, 4658
Bsp13I TCCGGA 1 cut(s) 3277
Bsp1407I TGTACA 3 cut(s) 106, 2170, 6121
Bsp19I CCATGG 2 cut(s) 1986, 5668
Bsp68I TCGCGA 1 cut(s) 2541
BspCNI CTCAG 9 cut(s) 247, 428, 1228, 4379, 5002, 5575, 5611, 5623, 5827
BspDI ATCGAT 2 cut(s) 2550, 4335
BspEI TCCGGA 1 cut(s) 3277
BspFNI CGCG 4 cut(s) 28, 2457, 2541, 3138
BspHI TCATGA 1 cut(s) 3238
BspLI GGNNCC 9 cut(s) 1715, 2037, 2349, 3819, 3966, 4114, 4684, 5405, 5445
BspMAI CTGCAG 2 cut(s) 4663, 4876
BspMI ACCTGC 6 cut(s) 82, 1471, 1541, 1579, 2441, 4887
BspQI GCTCTTC 2 cut(s) 618, 4912
BspT104I TTCGAA 2 cut(s) 50, 1076
BspT107I GGYRCC 2 cut(s) 2035, 4112
BspTI CTTAAG 3 cut(s) 2252, 3941, 5933
BsrDI GCAATG 8 cut(s) 598, 1260, 2167, 2684, 3224, 4291, 5248, 6063
BsrFI RCCGGY 1 cut(s) 2032
BsrGI TGTACA 3 cut(s) 106, 2170, 6121
BssAI RCCGGY 1 cut(s) 2032
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 7 cut(s) 405, 1475, 1717, 1986, 2122, 2923, 5668
Bst2UI CCWGG 8 cut(s) 726, 795, 848, 1317, 3401, 4326, 4792, 5825
Bst6I CTCTTC 6 cut(s) 307, 618, 1797, 2634, 3762, 4912
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 3 cut(s) 2252, 3941, 5933
BstAPI GCANNNNNTGC 3 cut(s) 3179, 4880, 5198
BstAUI TGTACA 3 cut(s) 106, 2170, 6121
BstBAI YACGTR 2 cut(s) 773, 5108
BstBI TTCGAA 2 cut(s) 50, 1076
BstC8I GCNNGC 9 cut(s) 1142, 1422, 2502, 2633, 3015, 3595, 3599, 5103, 5164
BstDSI CCRYGG 2 cut(s) 1986, 5668
BstENI CCTNNNNNAGG 1 cut(s) 2326
BstFNI CGCG 4 cut(s) 28, 2457, 2541, 3138
BstHHI GCGC 3 cut(s) 30, 2691, 5998
BstMAI GTCTC 2 cut(s) 702, 5995
BstMCI CGRYCG 1 cut(s) 2033
BstNI CCWGG 8 cut(s) 726, 795, 848, 1317, 3401, 4326, 4792, 5825
BstNSI RCATGY 4 cut(s) 678, 2240, 4780, 6230
BstSFI CTRYAG 6 cut(s) 114, 864, 1749, 4089, 4659, 4872
BstSLI GKGCMC 7 cut(s) 847, 2798, 3184, 3583, 3742, 4117, 4658
BstUI CGCG 4 cut(s) 28, 2457, 2541, 3138
BstV2I GAAGAC 3 cut(s) 5007, 5378, 5671
BstX2I RGATCY 8 cut(s) 1165, 1405, 2134, 2347, 4228, 4492, 4588, 6292
BstXI CCANNNNNNTGG 4 cut(s) 887, 1724, 2396, 5758
BstYI RGATCY 8 cut(s) 1165, 1405, 2134, 2347, 4228, 4492, 4588, 6292
Bsu15I ATCGAT 2 cut(s) 2550, 4335
Bsu36I CCTNAGG 1 cut(s) 4488
BsuI GTATCC 2 cut(s) 1036, 5568
BsuTUI ATCGAT 2 cut(s) 2550, 4335
BtgI CCRYGG 2 cut(s) 1986, 5668
BtgZI GCGATG 2 cut(s) 150, 2765
BtrI CACGTC 1 cut(s) 2930
BtsI GCAGTG 2 cut(s) 493, 1031
BtsIMutI CAGTG 9 cut(s) 493, 919, 1031, 1388, 2277, 2293, 2358, 4870, 5462
BtuMI TCGCGA 1 cut(s) 2541
BveI ACCTGC 6 cut(s) 82, 1471, 1541, 1579, 2441, 4887
Cac8I GCNNGC 9 cut(s) 1142, 1422, 2502, 2633, 3015, 3595, 3599, 5103, 5164
CaiI CAGNNNCTG 4 cut(s) 4880, 5423, 5834, 6038
CciI TCATGA 1 cut(s) 3238
CfoI GCGC 3 cut(s) 30, 2691, 5998
Cfr10I RCCGGY 1 cut(s) 2032
Cfr13I GGNCC 8 cut(s) 1198, 1298, 1807, 3397, 3817, 4301, 4962, 5620
ClaI ATCGAT 2 cut(s) 2550, 4335
CseI GACGC 3 cut(s) 2446, 3708, 4877
CsiI ACCWGGT 2 cut(s) 793, 1315
CspAI ACCGGT 1 cut(s) 2032
CspCI CAANNNNNGTGG 2 cut(s) 2905, 2940
DraI TTTAAA 4 cut(s) 589, 2805, 3379, 3799
DraIII CACNNNGTG 6 cut(s) 131, 797, 923, 1319, 1643, 3179
DrdI GACNNNNNNGTC 1 cut(s) 5710
DseDI GACNNNNNNGTC 1 cut(s) 5710
EaeI YGGCCR 1 cut(s) 4443
Eam1104I CTCTTC 6 cut(s) 307, 618, 1797, 2634, 3762, 4912
EarI CTCTTC 6 cut(s) 307, 618, 1797, 2634, 3762, 4912
EciI GGCGGA 1 cut(s) 3779
Eco130I CCWWGG 7 cut(s) 405, 1475, 1717, 1986, 2122, 2923, 5668
Eco147I AGGCCT 1 cut(s) 3404
Eco24I GRGCYC 1 cut(s) 2403
Eco32I GATATC 2 cut(s) 4212, 4384
Eco47I GGWCC 3 cut(s) 1198, 3397, 3817
Eco57I CTGAAG 9 cut(s) 470, 855, 1412, 1478, 2724, 2979, 3642, 4017, 5019
Eco81I CCTNAGG 1 cut(s) 4488
Eco88I CYCGRG 3 cut(s) 650, 3570, 5541
EcoNI CCTNNNNNAGG 1 cut(s) 2326
EcoO109I RGGNCCY 2 cut(s) 3817, 4301
EcoRI GAATTC 1 cut(s) 5769
EcoRII CCWGG 8 cut(s) 724, 793, 846, 1315, 3399, 4324, 4790, 5823
EcoRV GATATC 2 cut(s) 4212, 4384
EcoT14I CCWWGG 7 cut(s) 405, 1475, 1717, 1986, 2122, 2923, 5668
EcoT22I ATGCAT 1 cut(s) 6228
EcoT38I GRGCYC 1 cut(s) 2403
ErhI CCWWGG 7 cut(s) 405, 1475, 1717, 1986, 2122, 2923, 5668
FaqI GGGAC 6 cut(s) 2569, 3268, 3803, 3846, 5282, 5805
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 5469
FbaI TGATCA 2 cut(s) 3283, 5521
FblI GTMKAC 1 cut(s) 4185
FriOI GRGCYC 1 cut(s) 2403
GlaI GCGC 3 cut(s) 29, 2690, 5997
GsaI CCCAGC 2 cut(s) 2400, 5120
GsuI CTGGAG 5 cut(s) 357, 414, 1685, 2397, 5438
HapII CCGG 5 cut(s) 170, 2033, 2530, 3278, 5868
HgaI GACGC 3 cut(s) 2446, 3708, 4877
HhaI GCGC 3 cut(s) 30, 2691, 5998
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 28, 2689, 5996
HinP1I GCGC 3 cut(s) 28, 2689, 5996
HincII GTYRAC 7 cut(s) 1896, 2119, 2269, 3999, 4480, 5476, 5583
HindII GTYRAC 7 cut(s) 1896, 2119, 2269, 3999, 4480, 5476, 5583
HindIII AAGCTT 5 cut(s) 801, 1378, 1466, 3372, 5148
HpaI GTTAAC 1 cut(s) 4480
HpaII CCGG 5 cut(s) 170, 2033, 2530, 3278, 5868
Hpy99I CGWCG 2 cut(s) 28, 4947
HpyCH4IV ACGT 7 cut(s) 705, 772, 2929, 4022, 4600, 5107, 5333
HpySE526I ACGT 7 cut(s) 705, 772, 2929, 4022, 4600, 5107, 5333
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 28, 2689, 5996
Kpn2I TCCGGA 1 cut(s) 3277
KpnI GGTACC 1 cut(s) 2039
Ksp22I TGATCA 2 cut(s) 3283, 5521
KspAI GTTAAC 1 cut(s) 4480
LguI GCTCTTC 2 cut(s) 618, 4912
LmnI GCTCC 6 cut(s) 338, 1666, 4608, 5062, 5409, 6089
MabI ACCWGGT 2 cut(s) 793, 1315
MaeII ACGT 7 cut(s) 705, 772, 2929, 4022, 4600, 5107, 5333
MaeIII GTNAC 9 cut(s) 1246, 2302, 3043, 3145, 3439, 4566, 4865, 5013, 6180
MfeI CAATTG 2 cut(s) 4050, 4098
MflI RGATCY 8 cut(s) 1165, 1405, 2134, 2347, 4228, 4492, 4588, 6292
MhlI GDGCHC 8 cut(s) 847, 2403, 2798, 3184, 3583, 3742, 4117, 4658
MlsI TGGCCA 1 cut(s) 4445
MluI ACGCGT 1 cut(s) 2455
MluNI TGGCCA 1 cut(s) 4445
MlyI GAGTC 4 cut(s) 2520, 2716, 4699, 5021
MmeI TCCRAC 6 cut(s) 3121, 3469, 3869, 4566, 5878, 6223
Mox20I TGGCCA 1 cut(s) 4445
Mph1103I ATGCAT 1 cut(s) 6228
MroI TCCGGA 1 cut(s) 3277
MroXI GAANNNNTTC 2 cut(s) 1491, 4336
MscI TGGCCA 1 cut(s) 4445
MslI CAYNNNNRTG 9 cut(s) 318, 324, 924, 1134, 2057, 2784, 4651, 4775, 5673
Msp20I TGGCCA 1 cut(s) 4445
MspA1I CMGCKG 6 cut(s) 218, 1916, 1968, 2486, 4880, 6035
MspCI CTTAAG 3 cut(s) 2252, 3941, 5933
MspI CCGG 5 cut(s) 170, 2033, 2530, 3278, 5868
MunI CAATTG 2 cut(s) 4050, 4098
Mva1269I GAATGC 2 cut(s) 1493, 3421
MvaI CCWGG 8 cut(s) 726, 795, 848, 1317, 3401, 4326, 4792, 5825
MvnI CGCG 4 cut(s) 28, 2457, 2541, 3138
NciI CCSGG 2 cut(s) 2530, 5868
NcoI CCATGG 2 cut(s) 1986, 5668
NdeI CATATG 1 cut(s) 5469
NlaIV GGNNCC 9 cut(s) 1715, 2037, 2349, 3819, 3966, 4114, 4684, 5405, 5445
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 4 cut(s) 2302, 3145, 4865, 5013
NruI TCGCGA 1 cut(s) 2541
NsiI ATGCAT 1 cut(s) 6228
NspI RCATGY 4 cut(s) 678, 2240, 4780, 6230
NspV TTCGAA 2 cut(s) 50, 1076
PaeR7I CTCGAG 3 cut(s) 650, 3570, 5541
PagI TCATGA 1 cut(s) 3238
PaqCI CACCTGC 2 cut(s) 82, 4887
PceI AGGCCT 1 cut(s) 3404
PciI ACATGT 1 cut(s) 4776
PciSI GCTCTTC 2 cut(s) 618, 4912
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 2587
PctI GAATGC 2 cut(s) 1493, 3421
PdmI GAANNNNTTC 2 cut(s) 1491, 4336
Pfl23II CGTACG 1 cut(s) 3602
PflMI CCANNNNNTGG 4 cut(s) 3851, 4103, 4452, 5461
PinAI ACCGGT 1 cut(s) 2032
PleI GAGTC 4 cut(s) 2519, 2716, 4698, 5020
PpsI GAGTC 4 cut(s) 2519, 2716, 4698, 5020
Ppu21I YACGTR 2 cut(s) 773, 5108
PpuMI RGGWCCY 1 cut(s) 3817
PscI ACATGT 1 cut(s) 4776
PshBI ATTAAT 1 cut(s) 5535
PsiI TTATAA 3 cut(s) 180, 5021, 5154
Psp5II RGGWCCY 1 cut(s) 3817
Psp6I CCWGG 8 cut(s) 724, 793, 846, 1315, 3399, 4324, 4790, 5823
PspFI CCCAGC 2 cut(s) 2396, 5116
PspGI CCWGG 8 cut(s) 724, 793, 846, 1315, 3399, 4324, 4790, 5823
PspLI CGTACG 1 cut(s) 3602
PspN4I GGNNCC 9 cut(s) 1715, 2037, 2349, 3819, 3966, 4114, 4684, 5405, 5445
PspPI GGNCC 8 cut(s) 1198, 1298, 1807, 3397, 3817, 4301, 4962, 5620
PspPPI RGGWCCY 1 cut(s) 3817
PspXI VCTCGAGB 1 cut(s) 650
PsrI GAACNNNNNNTAC 2 cut(s) 2057, 2089
PstI CTGCAG 2 cut(s) 4663, 4876
PstNI CAGNNNCTG 4 cut(s) 4880, 5423, 5834, 6038
PsuI RGATCY 8 cut(s) 1165, 1405, 2134, 2347, 4228, 4492, 4588, 6292
PvuII CAGCTG 4 cut(s) 1916, 2486, 4880, 6035
RruI TCGCGA 1 cut(s) 2541
RseI CAYNNNNRTG 9 cut(s) 318, 324, 924, 1134, 2057, 2784, 4651, 4775, 5673
SapI GCTCTTC 2 cut(s) 618, 4912
Sau96I GGNCC 8 cut(s) 1198, 1298, 1807, 3397, 3817, 4301, 4962, 5620
ScaI AGTACT 3 cut(s) 1369, 3268, 5239
SchI GAGTC 4 cut(s) 2520, 2716, 4699, 5021
SduI GDGCHC 8 cut(s) 847, 2403, 2798, 3184, 3583, 3742, 4117, 4658
SexAI ACCWGGT 2 cut(s) 793, 1315
SfcI CTRYAG 6 cut(s) 114, 864, 1749, 4089, 4659, 4872
Sfr274I CTCGAG 3 cut(s) 650, 3570, 5541
SfuI TTCGAA 2 cut(s) 50, 1076
SinI GGWCC 3 cut(s) 1198, 3397, 3817
SlaI CTCGAG 3 cut(s) 650, 3570, 5541
SmiMI CAYNNNNRTG 9 cut(s) 318, 324, 924, 1134, 2057, 2784, 4651, 4775, 5673
SmlI CTYRAG 8 cut(s) 650, 1061, 2252, 3570, 3941, 5138, 5541, 5933
SmoI CTYRAG 8 cut(s) 650, 1061, 2252, 3570, 3941, 5138, 5541, 5933
SpeI ACTAGT 1 cut(s) 3337
SseBI AGGCCT 1 cut(s) 3404
SspI AATATT 4 cut(s) 2196, 4351, 5047, 6067
StuI AGGCCT 1 cut(s) 3404
StyI CCWWGG 7 cut(s) 405, 1475, 1717, 1986, 2122, 2923, 5668
TaiI ACGT 7 cut(s) 708, 775, 2932, 4025, 4603, 5110, 5336
TauI GCSGC 4 cut(s) 221, 1588, 5614, 5910
TscAI CASTG 9 cut(s) 493, 926, 1038, 1395, 2284, 2293, 2358, 4870, 5462
TseFI GTSAC 4 cut(s) 2302, 3145, 4865, 5013
Tsp45I GTSAC 4 cut(s) 2302, 3145, 4865, 5013
TspGWI ACGGA 8 cut(s) 12, 73, 1190, 2176, 2846, 3115, 5793, 6064
TspRI CASTG 9 cut(s) 493, 926, 1038, 1395, 2284, 2293, 2358, 4870, 5462
Van91I CCANNNNNTGG 4 cut(s) 3851, 4103, 4452, 5461
Vha464I CTTAAG 3 cut(s) 2252, 3941, 5933
VneI GTGCAC 3 cut(s) 2794, 3180, 3738
VpaK11BI GGWCC 3 cut(s) 1198, 3397, 3817
VspI ATTAAT 1 cut(s) 5535
XagI CCTNNNNNAGG 1 cut(s) 2326
XbaI TCTAGA 3 cut(s) 700, 1408, 2881
XceI RCATGY 4 cut(s) 678, 2240, 4780, 6230
XcmI CCANNNNNNNNNTGG 1 cut(s) 1267
XhoI CTCGAG 3 cut(s) 650, 3570, 5541
XmiI GTMKAC 1 cut(s) 4185
XmnI GAANNNNTTC 2 cut(s) 1491, 4336
ZrmI AGTACT 3 cut(s) 1369, 3268, 5239
Zsp2I ATGCAT 1 cut(s) 6228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.