Rh6CG468000

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013097
Physical Location & Seq
Forward (+)
63972758 .. 63990195
17438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG468000.1

Sequence Viewer

Length: 6540 bp
ATGGCGTCTGGTTCGCCTCCTCCGTCGCGCATCAATCAGTTTTTTGCTTCGAAGAAACGGAAGCCTGTATCACCTGCTATGAAGTCGGGGAGAAATGAAAAGGATGTAAAAACCGTAGAAGGTTCACCTAGTGCTAAGGCCACATTGGACGGTTATTTGCGGACATCGCCGGAGAGTTATAATGTGGCGAAACCTTTGTGCAAATTTGGTGATTCAGCGGCTTGGCAAGATCAAGTTAGAAGGAATTTGGCATCCGAGATGGATAATTCTTCGAAAAATGAATGTATGCAGCACCGTTTCTCTTCTCAACTGCACACTTATGCAAGTGAGGCTTCTGGAGCAAATCAGAAGGAAATAGCTAAGGGATTAACCAAGGAGGGTGATGTTGCAGCTGGAGGTTCTTCCAAGGATTACTCAGATTTTGCAGGAGGCGTGGAGAATGTAGAGCTGAAGAAGTTTGCAGCCGACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAGTTCTATGTCAGAGCTGAAAGTGAATGGTCATAAGAGACACCCTAGCCCATCTTTGCTTGATGAGGAGAGTAAGACATTTAAAAAGAGACATTTAGATAAATCCTATGAAGAACGTGAAACAATTGGTTCTACTGAGAAGAGCTATGAAGCAATGCAATCTGCTTGTGTTGATAAAAATAGGGTTACCATTCCTAATGAATTGTTCGAGCTTCAACAAACCTTGAAAACGTGCAGTAATACACCTAAATTGTCTCTAGACGTAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAATACGTGGTAGCTCATTTTCTTCACCTGGTGAAGCTTTTTGGAACGATGCAATTCAACTTGCTGATGGTCTATGTGCCCAGGCAGCAGAAGCAACTACAGCTTCAGATAGCCAATATCATGGTATAAACTCTTGCAATTTGAGAAATGCACACTGTGACGAAAAGTCAAAGGAAATGATGGATGAAGGTGAGCGTATGGGTAATGGTGCTGATTCAGAGCCAATGGTAAAGCATAGGAAGGACTTGCGTAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGATTTCTCTCGTGAGGACAAGATTTCGAATGAAAGTATGCCACATCATATAGATTCTTACAATTTGAAAAGCACGGCTCATGGAGGTGGCGAGCAATCTGAATCCAGTCTTATAGATCCTAGAGGTCTAAGACATGGTATTACTATGGTCTGTTGCAGCAAATCTCAAGCAAATCAAGGGCAAGATGTTGATTCTGTTACTGCAGTGACCAAAATGAAAGTGGATTTGTCTAGCAAGGATAATGGTGGCCTGACATTTTCTTCACCAGGTGATGATGTCGTTAAGTTGAATGGTAAAGGTGAATCTAGCGAAGCAAGTACTCCTTCAAGTTTTCTGCCACTGAAGGATTGTCTGGATCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCCAAGCTTTATCCTTGGCAGGTTGAGTGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACGAGTGCAGGTAAAAGTTTTGTTGCAGAAATTCTGATGCTGCGGCAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCAGAACATCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGGAACCAAGGTGGTGGTACACTTCCTAAAGACACTTCTGTAGCTGTCTGCACAATAGAAAAGGCAAACTTTTTAGTAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCTGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGACCCTGCTCATGGTTTGCAAATTGTTGGTATGAGTGCCACTATGCCAAATGTGGCAGCAGTTGCTGATTGGCTTCAAGCTGCCCTTTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATCAAAGTGGGGAACACCATTTATAACAAGAAAATGGAGATTATTAGAACAATCCCAAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCATGTTGTGGAACTGTGCAATGAGGTTGTACAAGAGGGTCTTTCCGTGTTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTGCAGTCAACACTCGCACTGATGACAGTGATTTTAATGATGTGACATTGGCTATTGATTCCTTACGAAGGTGTCCTGCTGGTTTGGATCCAGTATTAGAAGAAACCCTACCTTCTGGAGTTGCCTACCACCATGCTGGGCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAAGGCCTTGTACGCGTCTTAACTGCCACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGAGTCATTTTTCGTCAGCCCCGGATTGGTCGCGATTTTATCGATGGGACAAGGTACAGGCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTAATTTGCAAGCCAGAAGAGATCAAAAGGATCATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTTGTAGCAGGGGGAATTGTTCAAACTGCTAATGACATTCATCGCTATGTTAGGTGCACACTTTTAAACTCCACTAAACCTTTTCAGGATGTGGTGAAATCAGCACAGGAATCTATCCGCTGGTTGTGCCACAAGAAATTTCTAGAATGGAATGACGACACTAAGTTATACAATACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTGTGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGCTTTGTGCTTGCTTCTGATTTGCATCTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGCACAGTTCATGGAATTGTCTGCTCTTGACCAGTCCGTTGGCAATCGCGTTGGAGTGACAGAACAATTTTTGATGCGAATGGCACATGGTGCACCTATGCGGTCATCAAACAGGATGAGAGAAAGTACAAAAGCGTTGCATGGCAATTATGAAAGCCGACTTGGGACTACAAAGAGTACTGTGCTTCCGGATGATCAAACAGTTCGAGTTTGTAAACGGTTCTATGTTGCTCTCATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTCTGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGCCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACTATGTTTTGTGAAAGGCTCGGATGGCATGATCTGGAAGGCTTGGTTGGAAAATTTCAAAACCGTGTTTCATTTGGAGTGAGAGCAGAGATTGTAGAGCTGACTACAATTCCATTTGTCAAGGGTTCTAGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACGCCTTTAGCTATTGCTGAAGCATCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCATCGTGGGCAGCACAAGAAGGTTCAGCTCAAAGACGCGTCCAAGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATTGTTCTTGAAAAGGCTGAAGAGGCCAGAGTTGCTGCCTTCTCGGCTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCAACCATTATTTCCAACTGCTGGTGGAAGTCCCACTATGCAAGGAACAACAAATTCCTCTGGTGATGAAAATACTAGTAGCTTTGTTAAGGCCCATGTGGAGGATGCTCCTAAGCCATCTTTGGAAGGAAGGGTTCCTTCTGATAAGGTTGTATCAGGTGCGAAGTTGACAAAAACATCAGATATTGGAACGGTGGTGGCTTCAGGAGAAGTAAATTCAAGTGCTGTCATGCAAATTAAGTTTGGGGCTGAAAATTCTGTAGTACCAATTGATGGGTCTGGTGCCCTTGCTGATGAGCTAAATGCTACTGTTCATCAAACTAAAATTGCTGACTTGACCAACTCGATTACCGTAGACAGCTTTGGTGATAGAAATAGGATATCCAGCGAGTCTTTAGATCCTAAAAAGCAGGGAAGCTGTGAAGGGGAAAATTTGTCTGTTGGTGTTAAGGGCAATGCTTGTGAAAAAGGCCCTATTCATGCAATTTATACTCCTGGTGGAATAGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTTCATTTTGATATCCACTACAACAAAAGATCAGAATTGAACTCTGTTGCCCCCTTTGAAATACATGGAATGGCCATCTGTTGGGAAGATTCTCCTGTGTACTATGTTAACATTCCTAAGGATCTGCTGTGTTCTGGCAGCAGCAGTAAAGATGAAGGGTTGCGTTTGACTGCATCAGGTGACAGAACTAATGTGTTACCTCTTGATGATATGTTGGAGCTGGCTAAACGTAGATGGAGCAGGATTGGTGAAATCATGGGGAAAAGAGGTGTCAGGAAATTTACTTGGATGTTGAAAAGTCAGATTCAGGCACTGAAGTGCCCTGCAGTTCAAATACAGAGATTTGGTTGCCAGAGTCTTGCAGGGAAAAGTATTTGTTTTGAAATTATAGACACCTCGTTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTTTGGCCTGATGAGGAGAAAAATTCTAATCCAAACTTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGTGACACTGCAGCAGCTGCAAATCGAAACGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCTCAAACACGGGCCTTATGTTCTGTTCTGTGGAAGTTGCTTGTTTCGGAAGACCTTGCTGAGTCACTTATAAATATAGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGACATGGAGGGATGTCTGCAAGCACGTAATGTGCTGGGGAAAAAGCTAAGACATCTTGAGAAAGAAGCTTATAAACTGGCTGGCATGACATTTTCATTATATACAGCAGCAGATATTGCTAATGTGCTGTATGGACACTTAAAGCTGCCCATACCAGAGGTGCGCAACAAGGGAAAACAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGACGAGCATCCTATTATTCCCATCATCAAGGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGTTGGGATCAATTTGCTCACTTGCTAGGCTATCTGTGAAGACACAGAAGTACACATTACACGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGGATGAAAATGGAGGCAAAAGTGATGCTGATCATTACAAAATTAATGCTCGAGAATACTTTATACCTACTCAGGACAACTGGATACTGTTGACCGCAGATTATTCTCAGATAGAACTGCGGCTGATGGCCCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGTGATGTCTTCACCATGATTGCAGCTAGATGGACTGGAATGTCAGAAGATTGTATTAGTTCTGATGTACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTACGGAATGGGTGCTAATAGCCTTGCAGAACAACTGGATTGCAGTCCCCAGGCAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTCCCCGGAGTTGCTTCTTGGCTCAATGAAGCAGTTGGATATTGCCGCAAGAAAGGATATGTGGAAACCATTAAGGGAAGAAAACGATTTTTGTCTAAAATAAAATTTGGGAATAGCAAGGAAAAGTCAAAGGCGCAAAGACAGGCTGTAAATTCGATATGTCAGGGATCCGCTGCTGACATAATTAAGATTGCAATGATAAATATTTACTCCGTAATAGTTGATGGAGCTGAAAGGCCTGATTCGACTTCTCCACTTGCAACAAAGTTTCACAAACTGAAGGGCCGCTGCCGAATTCTATTGCAGGTACATGATGAATTGGTACTTGAAGTTGATCCTTCTGTGATAAAGGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAGTGCTGTTTCGCTTCTTGTTCCTATGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCTGACCAATATGAAGATCTGCCTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2179

Amino Acids

239.91

Weight (kDa)

7.25

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 523 - 699 1.1e-18 DEAD/DEAH box helicase
Helicase_C PF00271 829 - 904 5.7e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 909 - 1000 1.3e-24 Helix-turn-helix domain
POLQ_helical PF21099 1024 - 1208 1e-52 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1214 - 1288 5.3e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1751 - 2163 3e-110 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 180, 2190, 5171, 5304
AarI CACCTGC 2 cut(s) 82, 5037
AasI GACNNNNNNGTC 1 cut(s) 5893
Acc16I TGCGCA 1 cut(s) 5396
Acc36I ACCTGC 7 cut(s) 82, 1534, 1604, 1642, 2555, 5037, 6340
Acc65I GGTACC 1 cut(s) 2149
AccB1I GGYRCC 2 cut(s) 2149, 4226
AccB7I CCANNNNNTGG 4 cut(s) 3965, 4217, 4566, 5644
AccI GTMKAC 1 cut(s) 4299
AccII CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
AccIII TCCGGA 1 cut(s) 3391
AcoI YGGCCR 1 cut(s) 4557
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 6 cut(s) 131, 860, 986, 1382, 1706, 3293
AfiI CCNNNNNNNGG 9 cut(s) 1934, 2051, 2442, 2648, 3965, 4045, 4217, 4566, 5644
AflII CTTAAG 1 cut(s) 2366
AflIII ACRYGT 3 cut(s) 2569, 3814, 4926
AgeI ACCGGT 1 cut(s) 2146
AhdI GACNNNNNGTC 2 cut(s) 472, 994
AhlI ACTAGT 2 cut(s) 3451, 4019
AjiI CACGTC 1 cut(s) 3044
AjnI CCWGG 8 cut(s) 787, 856, 909, 1378, 3513, 4438, 4940, 6006
AjuI GAANNNNNNNTTGG 4 cut(s) 3348, 3380, 3585, 3617
AleI CACNNNNGTG 1 cut(s) 4801
AloI GAACNNNNNNTCC 2 cut(s) 5619, 5651
Alw21I GWGCWC 3 cut(s) 2912, 3298, 3856
Alw26I GTCTC 3 cut(s) 538, 589, 765
Alw44I GTGCAC 3 cut(s) 2908, 3294, 3852
AlwNI CAGNNNCTG 5 cut(s) 2105, 4798, 5030, 5606, 6017
Ama87I CYCGRG 1 cut(s) 5724
Aor13HI TCCGGA 1 cut(s) 3391
ApaLI GTGCAC 3 cut(s) 2908, 3294, 3852
ArsI GACNNNNNNTTYG 2 cut(s) 2677, 2709
AseI ATTAAT 1 cut(s) 5718
AsiGI ACCGGT 1 cut(s) 2146
Asp700I GAANNNNTTC 1 cut(s) 4450
Asp718I GGTACC 1 cut(s) 2149
AspLEI GCGC 4 cut(s) 30, 2805, 5397, 6181
AspS9I GGNCC 8 cut(s) 1870, 3511, 3931, 4036, 4415, 5112, 5803, 6328
AsuC2I CCSGG 2 cut(s) 2644, 6051
AsuII TTCGAA 3 cut(s) 50, 272, 1139
AvaI CYCGRG 1 cut(s) 5724
AvaII GGWCC 2 cut(s) 3511, 3931
AxyI CCTNAGG 1 cut(s) 4602
BaeGI GKGCMC 7 cut(s) 910, 2912, 3298, 3697, 3856, 4231, 4808
BalI TGGCCA 1 cut(s) 4559
BamHI GGATCC 2 cut(s) 2461, 6212
BanI GGYRCC 2 cut(s) 2149, 4226
BanII GRGCYC 1 cut(s) 2517
BarI GAAGNNNNNNTAC 2 cut(s) 2466, 2498
BauI CACGAG 1 cut(s) 1122
BbsI GAAGAC 3 cut(s) 5157, 5561, 5854
Bbv12I GWGCWC 3 cut(s) 2912, 3298, 3856
BceAI ACGGC 4 cut(s) 1203, 1857, 5059, 5076
BciT130I CCWGG 8 cut(s) 789, 858, 911, 1380, 3515, 4440, 4942, 6008
BciVI GTATCC 2 cut(s) 1099, 5751
BclI TGATCA 2 cut(s) 3397, 5704
BcnI CCSGG 2 cut(s) 2644, 6051
BcoDI GTCTC 3 cut(s) 538, 589, 765
BcuI ACTAGT 2 cut(s) 3451, 4019
BfmI CTRYAG 6 cut(s) 927, 1314, 1812, 4203, 4809, 5022
BfrI CTTAAG 1 cut(s) 2366
BfuAI ACCTGC 7 cut(s) 82, 1534, 1604, 1642, 2555, 5037, 6340
BfuI GTATCC 2 cut(s) 1099, 5751
BglI GCCNNNNNGGC 1 cut(s) 3905
BglII AGATCT 1 cut(s) 6523
BmcAI AGTACT 3 cut(s) 1432, 3382, 5422
Bme18I GGWCC 2 cut(s) 3511, 3931
BmeRI GACNNNNNGTC 2 cut(s) 472, 994
BmeT110I CYCGRG 1 cut(s) 5724
BmgBI CACGTC 1 cut(s) 3044
BmgT120I GGNCC 8 cut(s) 1870, 3511, 3931, 4036, 4415, 5112, 5803, 6328
BmiI GGNNCC 9 cut(s) 1778, 2151, 2463, 3933, 4080, 4228, 5588, 5628, 6214
BpiI GAAGAC 3 cut(s) 5157, 5561, 5854
BplI GAGNNNNNCTC 2 cut(s) 5807, 5839
BpmI CTGGAG 5 cut(s) 357, 414, 1748, 2511, 5621
Bpu10I CCTNAGC 4 cut(s) 135, 360, 2593, 4056
Bpu14I TTCGAA 3 cut(s) 50, 272, 1139
BpuEI CTTGAG 2 cut(s) 1263, 5309
BpuMI CCSGG 2 cut(s) 2644, 6051
Bsa29I ATCGAT 1 cut(s) 2664
BsaAI YACGTR 2 cut(s) 836, 5258
BsaBI GATNNNNATC 4 cut(s) 2253, 2335, 3141, 5703
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 2 cut(s) 2146, 3391
BsaXI ACNNNNNCTCC 4 cut(s) 2718, 2748, 5619, 5649
Bsc4I CCNNNNNNNGG 9 cut(s) 1934, 2051, 2442, 2648, 3965, 4045, 4217, 4566, 5644
Bse118I RCCGGY 1 cut(s) 2146
Bse21I CCTNAGG 1 cut(s) 4602
Bse3DI GCAATG 6 cut(s) 666, 2281, 2798, 4405, 5431, 6246
Bse8I GATNNNNATC 4 cut(s) 2253, 2335, 3141, 5703
BseAI TCCGGA 1 cut(s) 3391
BseBI CCWGG 8 cut(s) 789, 858, 911, 1380, 3515, 4440, 4942, 6008
BseCI ATCGAT 1 cut(s) 2664
BseJI GATNNNNATC 4 cut(s) 2253, 2335, 3141, 5703
BseLI CCNNNNNNNGG 9 cut(s) 1934, 2051, 2442, 2648, 3965, 4045, 4217, 4566, 5644
BseMI GCAATG 6 cut(s) 666, 2281, 2798, 4405, 5431, 6246
BseMII CTCAG 8 cut(s) 429, 633, 4494, 5151, 5759, 5795, 5805, 6009
BseRI GAGGAG 3 cut(s) 9, 587, 4976
BseSI GKGCMC 7 cut(s) 910, 2912, 3298, 3697, 3856, 4231, 4808
BseYI CCCAGC 2 cut(s) 2510, 5266
BsgI GTGCAG 8 cut(s) 296, 760, 1632, 1728, 1807, 1895, 3066, 5058
Bsh1236I CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
Bsh1285I CGRYCG 1 cut(s) 2147
BshNI GGYRCC 2 cut(s) 2149, 4226
BshTI ACCGGT 1 cut(s) 2146
BshVI ATCGAT 1 cut(s) 2664
BsiEI CGRYCG 1 cut(s) 2147
BsiHKAI GWGCWC 3 cut(s) 2912, 3298, 3856
BsiHKCI CYCGRG 1 cut(s) 5724
BsiSI CCGG 5 cut(s) 170, 2147, 2644, 3392, 6051
BsiWI CGTACG 1 cut(s) 3716
BslFI GGGAC 6 cut(s) 2683, 3382, 3917, 3960, 5465, 5988
BslI CCNNNNNNNGG 9 cut(s) 1934, 2051, 2442, 2648, 3965, 4045, 4217, 4566, 5644
BsmAI GTCTC 3 cut(s) 538, 589, 765
BsmFI GGGAC 6 cut(s) 2683, 3382, 3917, 3960, 5465, 5988
BsmI GAATGC 1 cut(s) 3535
BsoBI CYCGRG 1 cut(s) 5724
Bsp119I TTCGAA 3 cut(s) 50, 272, 1139
Bsp1286I GDGCHC 8 cut(s) 910, 2517, 2912, 3298, 3697, 3856, 4231, 4808
Bsp13I TCCGGA 1 cut(s) 3391
Bsp1407I TGTACA 1 cut(s) 2284
Bsp19I CCATGG 1 cut(s) 5851
Bsp68I TCGCGA 1 cut(s) 2655
BspCNI CTCAG 8 cut(s) 428, 634, 4493, 5152, 5758, 5794, 5806, 6010
BspDI ATCGAT 1 cut(s) 2664
BspEI TCCGGA 1 cut(s) 3391
BspFNI CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
BspLI GGNNCC 9 cut(s) 1778, 2151, 2463, 3933, 4080, 4228, 5588, 5628, 6214
BspMAI CTGCAG 3 cut(s) 1318, 4813, 5026
BspMI ACCTGC 7 cut(s) 82, 1534, 1604, 1642, 2555, 5037, 6340
BspQI GCTCTTC 2 cut(s) 641, 5062
BspT104I TTCGAA 3 cut(s) 50, 272, 1139
BspT107I GGYRCC 2 cut(s) 2149, 4226
BspTI CTTAAG 1 cut(s) 2366
BsrDI GCAATG 6 cut(s) 666, 2281, 2798, 4405, 5431, 6246
BsrFI RCCGGY 1 cut(s) 2146
BsrGI TGTACA 1 cut(s) 2284
BssAI RCCGGY 1 cut(s) 2146
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1122
BssT1I CCWWGG 7 cut(s) 372, 405, 1538, 1780, 2236, 3037, 5851
Bst2BI CACGAG 1 cut(s) 1122
Bst2UI CCWGG 8 cut(s) 789, 858, 911, 1380, 3515, 4440, 4942, 6008
Bst6I CTCTTC 6 cut(s) 307, 641, 1860, 2748, 3876, 5062
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2366
BstAPI GCANNNNNTGC 4 cut(s) 2102, 3293, 5030, 5348
BstAUI TGTACA 1 cut(s) 2284
BstBAI YACGTR 2 cut(s) 836, 5258
BstBI TTCGAA 3 cut(s) 50, 272, 1139
BstDSI CCRYGG 1 cut(s) 5851
BstEII GGTNACC 1 cut(s) 691
BstENI CCTNNNNNAGG 1 cut(s) 2440
BstFNI CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
BstHHI GCGC 4 cut(s) 30, 2805, 5397, 6181
BstMAI GTCTC 3 cut(s) 538, 589, 765
BstMCI CGRYCG 1 cut(s) 2147
BstNI CCWGG 8 cut(s) 789, 858, 911, 1380, 3515, 4440, 4942, 6008
BstNSI RCATGY 3 cut(s) 2354, 4930, 6461
BstPI GGTNACC 1 cut(s) 691
BstSFI CTRYAG 6 cut(s) 927, 1314, 1812, 4203, 4809, 5022
BstSLI GKGCMC 7 cut(s) 910, 2912, 3298, 3697, 3856, 4231, 4808
BstUI CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
BstV2I GAAGAC 3 cut(s) 5157, 5561, 5854
BstX2I RGATCY 8 cut(s) 1228, 1468, 2248, 2461, 4342, 4606, 6212, 6523
BstXI CCANNNNNNTGG 5 cut(s) 950, 1787, 2510, 3242, 5941
BstYI RGATCY 8 cut(s) 1228, 1468, 2248, 2461, 4342, 4606, 6212, 6523
Bsu15I ATCGAT 1 cut(s) 2664
Bsu36I CCTNAGG 1 cut(s) 4602
BsuI GTATCC 2 cut(s) 1099, 5751
BsuTUI ATCGAT 1 cut(s) 2664
BtgI CCRYGG 1 cut(s) 5851
BtgZI GCGATG 2 cut(s) 150, 2879
BtrI CACGTC 1 cut(s) 3044
BtsI GCAGTG 4 cut(s) 493, 1094, 1323, 5019
BtuMI TCGCGA 1 cut(s) 2655
BveI ACCTGC 7 cut(s) 82, 1534, 1604, 1642, 2555, 5037, 6340
CaiI CAGNNNCTG 5 cut(s) 2105, 4798, 5030, 5606, 6017
CfoI GCGC 4 cut(s) 30, 2805, 5397, 6181
Cfr10I RCCGGY 1 cut(s) 2146
Cfr13I GGNCC 8 cut(s) 1870, 3511, 3931, 4036, 4415, 5112, 5803, 6328
ClaI ATCGAT 1 cut(s) 2664
CseI GACGC 3 cut(s) 2560, 3805, 3822
CsiI ACCWGGT 2 cut(s) 856, 1378
CspAI ACCGGT 1 cut(s) 2146
CspCI CAANNNNNGTGG 2 cut(s) 3019, 3054
DraI TTTAAA 4 cut(s) 589, 2919, 3493, 3913
DraIII CACNNNGTG 6 cut(s) 131, 860, 986, 1382, 1706, 3293
DrdI GACNNNNNNGTC 1 cut(s) 5893
DriI GACNNNNNGTC 2 cut(s) 472, 994
DseDI GACNNNNNNGTC 1 cut(s) 5893
EaeI YGGCCR 1 cut(s) 4557
Eam1104I CTCTTC 6 cut(s) 307, 641, 1860, 2748, 3876, 5062
Eam1105I GACNNNNNGTC 2 cut(s) 472, 994
EarI CTCTTC 6 cut(s) 307, 641, 1860, 2748, 3876, 5062
Eco130I CCWWGG 7 cut(s) 372, 405, 1538, 1780, 2236, 3037, 5851
Eco147I AGGCCT 3 cut(s) 2562, 3518, 6283
Eco24I GRGCYC 1 cut(s) 2517
Eco32I GATATC 2 cut(s) 4326, 4498
Eco47I GGWCC 2 cut(s) 3511, 3931
Eco81I CCTNAGG 1 cut(s) 4602
Eco88I CYCGRG 1 cut(s) 5724
Eco91I GGTNACC 1 cut(s) 691
EcoNI CCTNNNNNAGG 1 cut(s) 2440
EcoO109I RGGNCCY 2 cut(s) 3931, 4415
EcoO65I GGTNACC 1 cut(s) 691
EcoRI GAATTC 2 cut(s) 5952, 6339
EcoRII CCWGG 8 cut(s) 787, 856, 909, 1378, 3513, 4438, 4940, 6006
EcoRV GATATC 2 cut(s) 4326, 4498
EcoT14I CCWWGG 7 cut(s) 372, 405, 1538, 1780, 2236, 3037, 5851
EcoT22I ATGCAT 1 cut(s) 6459
EcoT38I GRGCYC 1 cut(s) 2517
ErhI CCWWGG 7 cut(s) 372, 405, 1538, 1780, 2236, 3037, 5851
FaqI GGGAC 6 cut(s) 2683, 3382, 3917, 3960, 5465, 5988
FauNDI CATATG 1 cut(s) 5652
FbaI TGATCA 2 cut(s) 3397, 5704
FblI GTMKAC 1 cut(s) 4299
FriOI GRGCYC 1 cut(s) 2517
FspI TGCGCA 1 cut(s) 5396
GlaI GCGC 4 cut(s) 29, 2804, 5396, 6180
GsaI CCCAGC 2 cut(s) 2514, 5270
GsuI CTGGAG 5 cut(s) 357, 414, 1748, 2511, 5621
HapII CCGG 5 cut(s) 170, 2147, 2644, 3392, 6051
HgaI GACGC 3 cut(s) 2560, 3805, 3822
HhaI GCGC 4 cut(s) 30, 2805, 5397, 6181
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 4 cut(s) 28, 2803, 5395, 6179
HinP1I GCGC 4 cut(s) 28, 2803, 5395, 6179
HincII GTYRAC 7 cut(s) 1959, 2233, 2383, 4113, 4594, 5659, 5766
HindII GTYRAC 7 cut(s) 1959, 2233, 2383, 4113, 4594, 5659, 5766
HindIII AAGCTT 4 cut(s) 864, 1529, 3486, 5298
HpaI GTTAAC 1 cut(s) 4594
HpaII CCGG 5 cut(s) 170, 2147, 2644, 3392, 6051
Hpy99I CGWCG 2 cut(s) 28, 5097
HpyCH4IV ACGT 8 cut(s) 622, 737, 768, 835, 3043, 4714, 5257, 5516
HpySE526I ACGT 8 cut(s) 622, 737, 768, 835, 3043, 4714, 5257, 5516
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 4 cut(s) 28, 2803, 5395, 6179
Kpn2I TCCGGA 1 cut(s) 3391
KpnI GGTACC 1 cut(s) 2153
Ksp22I TGATCA 2 cut(s) 3397, 5704
KspAI GTTAAC 1 cut(s) 4594
LguI GCTCTTC 2 cut(s) 641, 5062
LmnI GCTCC 9 cut(s) 338, 1729, 4057, 4702, 4722, 5212, 5482, 5592, 6272
MabI ACCWGGT 2 cut(s) 856, 1378
MaeII ACGT 8 cut(s) 622, 737, 768, 835, 3043, 4714, 5257, 5516
MfeI CAATTG 2 cut(s) 630, 4212
MflI RGATCY 8 cut(s) 1228, 1468, 2248, 2461, 4342, 4606, 6212, 6523
MhlI GDGCHC 8 cut(s) 910, 2517, 2912, 3298, 3697, 3856, 4231, 4808
MlsI TGGCCA 1 cut(s) 4559
MluI ACGCGT 2 cut(s) 2569, 3814
MluNI TGGCCA 1 cut(s) 4559
MlyI GAGTC 5 cut(s) 2634, 2830, 4343, 4849, 5171
MmeI TCCRAC 6 cut(s) 3235, 3583, 3983, 4680, 6061, 6454
Mox20I TGGCCA 1 cut(s) 4559
Mph1103I ATGCAT 1 cut(s) 6459
MroI TCCGGA 1 cut(s) 3391
MroXI GAANNNNTTC 1 cut(s) 4450
MscI TGGCCA 1 cut(s) 4559
MslI CAYNNNNRTG 7 cut(s) 318, 1197, 2171, 2898, 4801, 4925, 5856
Msp20I TGGCCA 1 cut(s) 4559
MspA1I CMGCKG 9 cut(s) 218, 392, 1979, 2031, 2600, 2973, 5030, 6218, 6333
MspCI CTTAAG 1 cut(s) 2366
MspI CCGG 5 cut(s) 170, 2147, 2644, 3392, 6051
MunI CAATTG 2 cut(s) 630, 4212
Mva1269I GAATGC 1 cut(s) 3535
MvaI CCWGG 8 cut(s) 789, 858, 911, 1380, 3515, 4440, 4942, 6008
MvnI CGCG 5 cut(s) 28, 2571, 2655, 3252, 3816
NciI CCSGG 2 cut(s) 2644, 6051
NcoI CCATGG 1 cut(s) 5851
NdeI CATATG 1 cut(s) 5652
NlaIV GGNNCC 9 cut(s) 1778, 2151, 2463, 3933, 4080, 4228, 5588, 5628, 6214
NmeAIII GCCGAG 1 cut(s) 3884
NmuCI GTSAC 7 cut(s) 986, 1318, 2416, 3259, 4664, 5015, 5163
NruI TCGCGA 1 cut(s) 2655
NsbI TGCGCA 1 cut(s) 5396
NsiI ATGCAT 1 cut(s) 6459
NspI RCATGY 3 cut(s) 2354, 4930, 6461
NspV TTCGAA 3 cut(s) 50, 272, 1139
OliI CACNNNNGTG 1 cut(s) 4801
PaeR7I CTCGAG 1 cut(s) 5724
PaqCI CACCTGC 2 cut(s) 82, 5037
PceI AGGCCT 3 cut(s) 2562, 3518, 6283
PciI ACATGT 1 cut(s) 4926
PciSI GCTCTTC 2 cut(s) 641, 5062
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 2701
PctI GAATGC 1 cut(s) 3535
PdmI GAANNNNTTC 1 cut(s) 4450
Pfl23II CGTACG 1 cut(s) 3716
PflMI CCANNNNNTGG 4 cut(s) 3965, 4217, 4566, 5644
PinAI ACCGGT 1 cut(s) 2146
PleI GAGTC 5 cut(s) 2633, 2830, 4342, 4848, 5170
PpsI GAGTC 5 cut(s) 2633, 2830, 4342, 4848, 5170
Ppu21I YACGTR 2 cut(s) 836, 5258
PpuMI RGGWCCY 1 cut(s) 3931
PscI ACATGT 1 cut(s) 4926
PshBI ATTAAT 1 cut(s) 5718
PsiI TTATAA 4 cut(s) 180, 2190, 5171, 5304
Psp5II RGGWCCY 1 cut(s) 3931
Psp6I CCWGG 8 cut(s) 787, 856, 909, 1378, 3513, 4438, 4940, 6006
PspEI GGTNACC 1 cut(s) 691
PspFI CCCAGC 2 cut(s) 2510, 5266
PspGI CCWGG 8 cut(s) 787, 856, 909, 1378, 3513, 4438, 4940, 6006
PspLI CGTACG 1 cut(s) 3716
PspN4I GGNNCC 9 cut(s) 1778, 2151, 2463, 3933, 4080, 4228, 5588, 5628, 6214
PspPI GGNCC 8 cut(s) 1870, 3511, 3931, 4036, 4415, 5112, 5803, 6328
PspPPI RGGWCCY 1 cut(s) 3931
PstI CTGCAG 3 cut(s) 1318, 4813, 5026
PstNI CAGNNNCTG 5 cut(s) 2105, 4798, 5030, 5606, 6017
PsuI RGATCY 8 cut(s) 1228, 1468, 2248, 2461, 4342, 4606, 6212, 6523
PvuII CAGCTG 4 cut(s) 392, 1979, 2600, 5030
RruI TCGCGA 1 cut(s) 2655
RseI CAYNNNNRTG 7 cut(s) 318, 1197, 2171, 2898, 4801, 4925, 5856
SapI GCTCTTC 2 cut(s) 641, 5062
Sau96I GGNCC 8 cut(s) 1870, 3511, 3931, 4036, 4415, 5112, 5803, 6328
ScaI AGTACT 3 cut(s) 1432, 3382, 5422
SchI GAGTC 5 cut(s) 2634, 2830, 4343, 4849, 5171
SduI GDGCHC 8 cut(s) 910, 2517, 2912, 3298, 3697, 3856, 4231, 4808
SexAI ACCWGGT 2 cut(s) 856, 1378
SfcI CTRYAG 6 cut(s) 927, 1314, 1812, 4203, 4809, 5022
Sfr274I CTCGAG 1 cut(s) 5724
SfuI TTCGAA 3 cut(s) 50, 272, 1139
SinI GGWCC 2 cut(s) 3511, 3931
SlaI CTCGAG 1 cut(s) 5724
SmiMI CAYNNNNRTG 7 cut(s) 318, 1197, 2171, 2898, 4801, 4925, 5856
SmlI CTYRAG 4 cut(s) 1278, 2366, 5288, 5724
SmoI CTYRAG 4 cut(s) 1278, 2366, 5288, 5724
SpeI ACTAGT 2 cut(s) 3451, 4019
SseBI AGGCCT 3 cut(s) 2562, 3518, 6283
SspI AATATT 4 cut(s) 2310, 4465, 5197, 6250
StuI AGGCCT 3 cut(s) 2562, 3518, 6283
StyI CCWWGG 7 cut(s) 372, 405, 1538, 1780, 2236, 3037, 5851
TaiI ACGT 8 cut(s) 625, 740, 771, 838, 3046, 4717, 5260, 5519
TatI WGTACW 9 cut(s) 1430, 1684, 2284, 2734, 3329, 3380, 4584, 5420, 5565
TauI GCSGC 5 cut(s) 221, 1651, 5797, 6093, 6333
TseFI GTSAC 7 cut(s) 986, 1318, 2416, 3259, 4664, 5015, 5163
Tsp45I GTSAC 7 cut(s) 986, 1318, 2416, 3259, 4664, 5015, 5163
TspGWI ACGGA 6 cut(s) 12, 73, 2290, 3229, 5976, 6247
Van91I CCANNNNNTGG 4 cut(s) 3965, 4217, 4566, 5644
Vha464I CTTAAG 1 cut(s) 2366
VneI GTGCAC 3 cut(s) 2908, 3294, 3852
VpaK11BI GGWCC 2 cut(s) 3511, 3931
VspI ATTAAT 1 cut(s) 5718
XagI CCTNNNNNAGG 1 cut(s) 2440
XbaI TCTAGA 4 cut(s) 763, 1471, 2995, 3683
XceI RCATGY 3 cut(s) 2354, 4930, 6461
XcmI CCANNNNNNNNNTGG 1 cut(s) 1330
XhoI CTCGAG 1 cut(s) 5724
XmiI GTMKAC 1 cut(s) 4299
XmnI GAANNNNTTC 1 cut(s) 4450
ZrmI AGTACT 3 cut(s) 1432, 3382, 5422
Zsp2I ATGCAT 1 cut(s) 6459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.